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本研究为了探讨恶性黑色素瘤的临床诊断及鉴别诊断要点,通过组织病理学检查和免疫组织化学染色方法对两例犬恶性黑色素瘤进行诊断。组织病理学检查发现瘤细胞排列紊乱,细胞主要呈梭形和上皮样两种细胞形态,可见瘤巨细胞、核分裂相,肿瘤细胞胞质中可见色素颗粒,细胞异型性明显,核仁较大,有丝分裂活跃,属于恶性黑色素瘤典型的组织学表现。免疫组织化学染色发现六种肿瘤标记物结果显示为CK（-）、Vimentin（+）、S-100（+）、CD34（-）、SMA-α（-）、KI-67（+）。通过组织病理学检查和免疫组化染色结果可以诊断为恶性黑色素瘤。 相似文献
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动物上皮细胞容易被多种病毒感染,上皮细胞表面标记物的检测可以作为表征上皮细胞状态的一种手段,但是大多数上皮细胞表面标记物也会在上皮来源的肿瘤细胞中表达。为了选择高效、独特的上皮标记物,采用蛋白质印迹法和间接免疫荧光技术,在牛肾上皮细胞(KE)、舌上皮细胞(TE)、甲状腺上皮细胞(TYE)以及A549细胞和SW480细胞中,检测了AE3(basic cytokeratin)、AE5(CK3/2p)、CK7、LH1(CK1/10)、EMA(epithelial membrane antigen)、DesmoplakinⅠ/Ⅱ(DSPⅠ/Ⅱ)、E-cadherin和ZO1(zona occludens-1)等8种上皮标记物的表达。结果显示,与阴性对照相比,AE3、AE5、CK7、EMA及DSPⅠ/Ⅱ在上皮细胞和上皮细胞来源的A549、SW480肿瘤细胞中表达良好;E-cadherin仅在上皮细胞中表达;LH1(CK1/10)和ZO1在所有被检测的细胞中都没有表达。结果表明,AE3、AE5、CK7、EMA及DSPⅠ/Ⅱ可以作为有效的上皮细胞及上皮来源的肿瘤细胞标记物,E-cadherin可以作为特异性的上皮细胞标记物。 相似文献
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肝胆管细胞癌是来源于肝胆管上皮细胞的恶性肿瘤,在多物种中都有发生。笔者运用组织病理学和免疫组织化学的方法,对2例犬肝肿瘤进行了诊断。结果显示：肝肿瘤眼观均为圆球形白色肿块,组织病理学检查肿瘤组织由呈腺管状排列的瘤细胞构成,有的腺管中央有坏死脱落的细胞,有的有黏液样物质,腺管间以薄的纤维结缔组织分隔,瘤组织有大片的坏死。瘤细胞多呈卵圆形,细胞核大、卵圆形,核分裂象多见,瘤细胞的CK19阳性表达,而CK18和AFP呈阴性表达。根据组织病理学和免疫组织化学检查结果判断,2例犬肝肿瘤为肝胆管细胞癌,本研究为犬肝肿瘤诊断积累了病理学资料。 相似文献
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对犬的皮脂腺瘤、肛周腺腺瘤、肛周腺癌、毛基质瘤、间叶性软骨肉瘤、腺样型基底细胞上皮瘤、囊样型基底细胞上皮瘤、水母样型基底细胞上皮瘤、混合型基底细胞上皮瘤、基底鳞状细胞上皮瘤、甲状腺腺瘤、肥大细胞瘤、组织细胞瘤、乳腺良性混合瘤(软骨腺瘤)、乳腺良性混合瘤(肌上皮腺瘤)、恶性肌上皮细胞瘤、乳腺恶性混合瘤(癌肉瘤)、汗腺囊性增生、汗腺腺瘤、汗腺癌等20种24例肿瘤(良性肿瘤14种,恶性肿瘤6种)作了详细的病理组织学描述。 相似文献
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试验旨在分析肽能神经递质蛋白基因产物9.5（protein gene product 9.5,PGP9.5）和神经肽Y（neuropeptide Y,NPY）在犬隐睾及睾丸肿瘤中的分布和表达,并与同年龄正常睾丸组织进行比较,为认识犬睾丸肿瘤恶变临床诊断提供参考。应用HE染色、Masson三色染色、Gomori银浸染、甲苯胺蓝染色观察各组织中网状纤维、胶原纤维及肥大细胞等组织特征,采用免疫组织化学SP法及免疫荧光法结合IPP统计分析PGP9.5和NPY在组织中的表达及定位。结果显示,正常犬睾丸生精上皮由4~7层生精细胞及Sertoli细胞构成,间质组织胶原纤维和网状纤维分布稀疏。隐睾生精小管基底膜胶原纤维厚度增加,Sertoli细胞核浓缩位于生精小管基底,间质网状纤维增多。睾丸肿瘤组织结构不清晰,胶原纤维和网状纤维无规则分布,肥大细胞较正常组及隐睾组显著增多。免疫荧光定位表明,PGP9.5在正常睾丸Leydig细胞中呈中等阳性表达,生精细胞中无明显表达;隐睾Leydig细胞及生精细胞中呈强阳性表达;睾丸肿瘤中偶有表达。NPY在正常睾丸Leydig细胞中偶见阳性表达,生精细胞中无表达;隐睾Leydig细胞及生精上皮中无表达,间质小血管管壁呈高密度强阳性表达;睾丸肿瘤组织中无明显表达。免疫组化统计表明,睾丸肿瘤组织中PGP9.5和NPY较正常组极显著降低（P<0.01）,隐睾组PGP9.5和NPY表达显著或极显著增加（P<0.05;P<0.01）。因此,犬隐睾时PGP9.5及NPY的表达增高,提示犬隐睾时已有发展为肿瘤的趋势,且与肿瘤恶变程度相关。 相似文献
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采用联合消化液对妊娠8～10周龄的胎儿胎盘组织进行了消化,获得单细胞悬液,然后将滋养层细胞分离纯化,探索其培养的最适FBS浓度及最适pH,并观察其大体形态、胞核的特点、细胞角蛋白7(Cytokeratin,CK7)和波形蛋白(Vimentin,Vim)表达等。结果显示,牦牛滋养层细胞在pH6.8～7.0、20%FBS的DMEM/F12培养基条件下适宜传代培养;该细胞为上皮样细胞形态,呈片状铺展生长;细胞角蛋白染色阳性,波形蛋白染色阴性;阳性细胞率达90%,台盼蓝排斥试验显示活细胞率超过95%。 相似文献
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针对目前山东省出现的某些商品蛋雏鸡皮肤增生、结痂为特征的致死性皮肤病,进行了流行病学调查、剖检、病理组织学及免疫组织化学检测.结果显示:该病仪在某场家、某批次的海兰褐商品蛋雏鸡中发现,多在5日龄左右发现病变,病程1月左右,病鸡最终衰竭死亡,发病率1%～10%不等;病初可见皮肤多发性黄色结节,随后结节融合,导致皮肤增厚、结痂,内脏器官无肉眼可见肿瘤病变;病理组织学观察,真皮层存在不同分化状态的毛囊样瘤细胞集团,瘤细胞有明显的异型性,病灶呈浸润性生长,侵犯神经及真皮下肌肉组织,伴有肌样纤维结缔组织大量增生;病变部皮肤表皮细胞显著增生,增生的基底细胞也有一定的异型性;免疫组化检测,肿瘤细胞呈广谱细胞角蛋白(CK)阳性;波形蛋白(Vim)、高分子量细胞角蛋白(CKHMW)、低分子量细胞角蛋白(CKLMW)、P53、Ki-67阴性;真皮层增生的肌样纤维结缔组织主要为平滑肌.该皮肤肿瘤初步诊断为毛源性上皮细胞恶性肿瘤. 相似文献
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为了探讨犬的乳腺肿瘤预后情况,试验结合犬自发性乳腺肿瘤临床症状进行细胞异型筛查、穿刺切片免疫组化或流式细胞仪细胞免疫分析检测犬外周血细胞异型后乳腺肿瘤的预后情况,对可疑病例犬采血、涂片、穿刺,用流式细胞仪进行检测。结果表明:犬的外周血中循环细胞异型数量超过5%;CK19阳性;流式细胞仪检测循环肿瘤细胞数(CTC)≥5,犬的肿瘤即有预后不良;细胞异型检查数量与肿瘤的预后不良有一定相关性;CK19阳性与肿瘤的大小相关;流式细胞仪检测的阳性率与预后不良相关。 相似文献
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Chiara Brachelente Ilaria Porcellato Monica Sforna Elvio Lepri Luca Mechelli Laura Bongiovanni 《Veterinary dermatology》2013,24(1):188-e41
Background – Although cutaneous stem cells have been implicated in skin tumourigenesis in humans, no studies have been conducted to elucidate the presence and the possible role of stem cells in hair follicle tumours in the dog. Hypothesis – Stem cell markers are expressed in canine epidermal and follicular tumours and can be used to better understand the biology and origin of these tumours. Animals and Methods – In the present study, normal skin sections and 44 follicular tumours were retrospectively investigated for the immunohistochemical expression of keratin 15 (K15) and nestin. In addition, 30 squamous cell carcinomas were evaluated for K15 expression. Results – In normal skin, K15 and nestin were expressed in the outer root sheath cells of the isthmic portion of the hair follicle (bulge region), and K15 expression was also scattered in the basal cell layer of the epidermis. Infundibular keratinizing acanthomas, pilomatricomas and squamous cell carcinomas were mostly negative for K15, trichoblastomas were moderately to strongly positive, tricholemmomas were either negative or strongly positive, and trichoepitheliomas had heterogeneous staining. Nestin expression was generally faint in all follicular tumours. Conclusions and clinical importance – Our results show that K15 can be a reliable marker for investigating the role of stem cells in hair follicle tumours of the dog, while nestin was judged to be a nonoptimal marker. Furthermore, our study suggests that hair follicle stem cells are present in the bulge region of hair follicles and could possibly play a role in tumourigenesis of canine tumours originating from this portion of the follicle, namely trichoblastomas, tricholemmomas and trichoepitheliomas. The loss of K15 expression in squamous cell carcinomas compared with normal skin suggests that this event could be important in the malignant transformation. 相似文献
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Hair follicle neoplasms occur in many different species, including humans. In domestic animals, they are most common in dogs. Most hair follicle tumors are benign, but malignant neoplasms can also occur. To diagnose hair follicle neoplasms, a thorough knowledge of follicular anatomy is important, given that follicular tumors are classified according to the differentiation pattern seen in the corresponding part of the normal hair follicle. This review focuses on the key diagnostic features of hair follicle tumors and follicular cysts in dogs and cats. 相似文献
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Pascucci L Mercati F Gargiulo AM Pedini V Sorbolini S Ceccarelli P 《Veterinary dermatology》2006,17(4):244-251
It is widely documented that a pool of multipotent stem cells located in humans and mice hair follicle outer root sheath (bulge region) is involved in the restoration of the whole follicular unit during each anagen phase. To the authors' knowledge, data regarding the location and characterization of hair follicle stem compartment in dogs have not been reported in the recent relevant literature. In this study, we investigated the haematopoietic stem and progenitor cell antigen CD34 as a marker of putative stem cells located in a bulge-like region of canine hair follicles. The presence of CD34 mRNA and glycoprotein was assessed on formalin-fixed, paraffin-embedded canine skin samples by in situ hybridization technique and by standard immunohistochemistry, respectively. A strong expression of CD34 mRNA and glycoprotein was observed in a well-defined area of the hair follicle isthmic region and appeared uniformly concentrated at the level of the basal layer of the outer root sheath. These findings provide compelling support to the hypothesis that in dogs, a subpopulation of basal keratinocytes located in the hair follicle isthmic region and characterized by the selective expression of CD34 is potentially associated with the stem cell compartment of this skin appendage. 相似文献
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Kato K Uchida K Nibe K Tateyama S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(3):233-239
The expressions of cytokeratin 8 and 18 (CK8 and CK18) in the normal canine skin (2 cases) and cutaneous adnexal tumors (127 cases) were investigated immunohistochemically. In the normal skin, co-expression of CK8/18 was found in the glandular epithelium of apocrine sweat glands, and single CK8-immunoreactivity was detected occasionally in the external root sheath at the isthmus and suprabulbar regions of the hair follicles. Neoplastic glandular epithelial cells in all apocrine gland tumors (21/21 cases, 100%) had co-expression of CK8/18. In trichoblastomas (27/28 cases, 96%), most neoplastic cells were diffusely positive for CK8, but those were negative for CK18. Single CK8-expression was also observed in basaloid neoplastic cells in several cases of trichoepitheliomas (7/19 cases, 37%) and pilomatricoma (1/7 cases, 14%). In several cases of trichoblastomas (4/28 cases, 14%) and trichoepitheliomas (2/19 cases, 11%), tumor cells forming glandular structures had co-expression of CK8/18. There were no positive reactions for both CK8 and 18 in infundibular keratinizing acanthomas, and sebaceous and hepatoid gland tumors. The present findings indicate that co-expression of CK8/18 is a specific feature of apocrine sweat glands and single CK8-expression represents the natures of external root sheath or pluripotential stem cells. Thus, the combination of CK8- and 18-immunostainings may have the utility to confirm the directions of differentiation in canine cutaneous adnexal tumors providing a reliable hallmark for histopathological diagnoses. 相似文献
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Diagnosis of persistent bovine viral diarrhea virus infection by immunohistochemical staining of formalin-fixed skin biopsy specimens. 总被引：5，自引：0，他引：5
B L Njaa E G Clark E Janzen J A Ellis D M Haines 《Journal of veterinary diagnostic investigation》2000,12(5):393-399
The objective of this study was to evaluate the efficacy of immunohistochemical (IHC) staining for diagnosis of persistent bovine viral diarrhea virus (BVDV) infection using formalin-fixed, paraffin-embedded skin biopsy specimens. Skin from 41 of 42 calves shown to be persistently infected (PI) with BVDV by repeated virus isolation more than 3 weeks apart were immunohistochemically positive for BVDV antigen. Positive IHC staining was most pronounced in the keratinocytes and in hair follicle epithelium, hair matrix cells of the hair bulb, and the dermal papilla. All of the skin sections from 10 calves experimentally infected postnatally with BVDV (10(5) median tissue culture infective doses [TCID50]) and biopsied on days 0, 5, 7, and 9 postinfection were negative for viral antigen. Ten calves from a second group experimentally infected with a higher dose of BVDV (10(8) TCID50) were biopsied when viremic between 10 and 14 days postinfection and 4 calves exhibited positive IHC staining for BVDV; however, staining in these skin biopsies was confined to small foci in the nonfollicular epidermis and follicular ostia. This staining was distinct from that observed in skin obtained from PI cattle. Skin biopsy represents an effective method for identifying animals PI with BVDV. 相似文献
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Alopecia areata in C3H/HeJ mice involves leukocyte-mediated root sheath disruption in advance of overt hair loss 总被引：2，自引：0，他引：2
McElwee KJ Silva K Boggess D Bechtold L King LE Sundberg JP 《Veterinary pathology》2003,40(6):643-650
Alopecia areata (AA) can be induced in C3H/HeJ mice by grafting full-thickness AA-affected skin. An 8- to 12-week delay between surgery and overt hair loss onset provides an opportunity to examine disease pathogenesis. Normal haired C3H/HeJ mice were sham-grafted or grafted with AA-affected skin. Mice were euthanatized 2, 4, 6, 8, 10, and 12 weeks after surgery along with chronic AA-affected mice as a positive control. Until 6 weeks after grafting, inflammation was only evident around anagen-stage hair follicles in host skin adjacent to but not distant from the AA-affected graft. From 8 weeks on, AA-grafted but not sham-grafted mice exhibited a diffuse dermal inflammation at distant sites that progressively focused on anagen-stage hair follicles at 10 and 12 weeks. Perifollicular inflammation was primarily composed of CD4+ and CD8+ cells associated with follicular epithelium intercellular adhesion molecule -1 expression. Only CD8+ cells penetrated intrafollicularly by 12 weeks after surgery, although both CD4+ and CD8+ intrafollicular cells were observed in chronic AA-affected mice. Under electron microscopy, intrafollicular lymphocyte and macrophage infiltration associated with hair follicle dystrophy was prominent 10 weeks after surgery, primarily within the differentiating outer and inner root sheaths. This study shows that focal follicular inflammation develops some time in advance of overt hair loss and focuses on the differentiating root sheaths in C3H/HeJ mice. The severity of inflammation and the degree of hair follicle dystrophy induced by the infiltrate appear to reach a threshold level before overt hair loss occurs. 相似文献
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采用0．2％中性蛋白酶Ⅱ和0．25％胰酶：0．02％EDTA（1：1）“两步酶”消化法从羊驼背部皮肤分离得到羊驼毛囊干细胞。用无血清角质细胞培养基（K-SFM）培养进行形态学观察、细胞生长曲线克隆形成率及免疫组化染色检测。结果显示，细胞生长曲线表明，不同代次毛囊干细胞接种前3d生长缓慢，4～6d进入倍增期，有无限增殖的趋势，所分离的羊驼毛囊干细胞具备毛囊干细胞特征（体积小、立体感强），呈现未分化特征；CK19、CK15、81-integrin和CD34免疫组化染色阳性；第3、5、7、9代克隆形成率分别为（32．7±2．27）％、（47．0±3．46）％、（46．3±3．18）％和（43．3±3．76）％。结果表明，用“两步酶”消化法成功分离获得羊驼毛囊干细胞，无血清角质细胞培养基（K—SFM）可使羊驼毛囊干细胞体外维持未分化状态并传代至12代。 相似文献
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Laura Bongiovanni Alessandra D’Andrea Mariarita Romanucci Daniela Malatesta Melissa Candolini Leonardo D. Salda Luca Mechelli Monica Sforna Chiara Brachelente 《Veterinary dermatology》2013,24(1):195-e43
Background – Epithelial‐to‐mesenchymal transition (EMT) is a multistep process, important in tumour invasion and metastasis, characterized by loss of epithelial markers, redistribution of β‐catenin and gain of mesenchymal markers. Hyposthesis/Objectives – Our aim was to investigate the immunohistochemical aberrant expression of cytokeratin, vimentin, survivin and heat shock protein 72 (Hsp72) in canine cutaneous epithelial tumours, to understand the association of expression of these molecules with features of malignancy and their role in the EMT phenotype. Methods – Ten canine squamous cell carcinomas (SCCs; one with lymph node metastasis), 30 canine hair follicle tumours (six pilomatricomas, eight infundibular keratinizing acanthomas, six trichoepitheliomas and 10 trichoblastomas) and five normal skin samples were investigated by immunohistochemistry using specific anti‐vimentin, ‐cytokeratin, ‐survivin and ‐Hsp72 antibodies. A semi‐quantitative method was used to analyse the results, as follows: 0 to <5%; ≥5 to <10%; ≥10 to <25%; and ≥25% of positive cells. Immunofluorescence was performed to investigate survivin–vimentin and survivin–Hsp72 colocalization in selected SCCs. Results – In malignant hair follicle tumours and SCCs, a reduced intensity of cytokeratin and increased survivin and Hsp72 expression were observed. In SCCs, loss of cytokeratin expression and vimentin immunolabelling, suggestive of the EMT phenotype, were evident in <5% of neoplastic cells in the front of tumour invasion. In the same areas, strong nuclear survivin and cytoplasmic Hsp72 staining was evident, often colocalizing. Only a few neoplastic cells in the front of tumour invasion showed vimentin–survivin colocalization. Conclusions and clinical importance – A possible simultaneous involvement of survivin and Hsp72 in tumour invasion and the multistep process of EMT of cutaneous epithelial tumours of dogs is suggested. 相似文献
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Chi-Ho Yu Du-Na Hwang Ji-Young Yhee Jong-Hyuk Kim Keum-Soon Im Whan-Gook Nho Young-Soo Lyoo Jung-Hyang Sur 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(1):1-7
Primary testicular tumors are the most common causes of cancer in male dogs. Overall, the majority of canine patients should be cured by testicular surgery. However, tumor markers are not well-known in veterinary medicine. We sought to determine using immunohistochemistry whether the combined human testicular tumor markers (placental alkaline phosphatase, OCT3/4, CD30, alpha-fetoprotein, inhibin-alpha, vimentin, c-KIT, and desmin) are expressed in canine seminomas and Sertoli cell tumors (SCTs). We examined 35 canine testicular tumors, 20 seminomas and 15 SCTs. c-KIT was expressed markedly in canine seminomas. Both inhibin-alpha and vimentin were expressed significantly in canine SCTs. The results of this study demonstrate differences and similarities between tumor marker expression of testicular tumors in dogs and humans. All the main markers in current routine use are discussed as well as potential useful markers for benign and malignant tumors, and tumor progression. 相似文献