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1.
《畜牧与兽医》2016,(4):76-78
采用免疫组织化学SABC-AP法探究波形蛋白在黄羽肉鸡肠道的分布规律。结果:免疫组织化学显示,波形蛋白在鸡小肠黏膜上皮、小肠腺细胞腔面、黏膜下神经丛和肌间神经丛均呈强阳性表达;在黏膜的固有膜呈阳性表达,肌间神经丛的血管壁周围呈强阳性表达;波形蛋白在鸡大肠黏膜上皮、大肠腺呈阳性表达,在固有膜、黏膜下神经丛和肌间神经丛均呈强阳性表达。波形蛋白是神经胶质细胞的标记物之一,其在鸡肠道的分布特征表明肠神经胶质细胞(EGCs)不仅对肠神经系统(ENS)的肠神经节细胞起支持、营养和保护作用,而且可能参与肠黏膜屏障、肠腺分泌、肠道物质的分泌与吸收、肠蠕动及血液的供应调节等诸多功能。 相似文献
2.
为了探究胶质纤维酸性蛋白(GFAP)在新疆哈萨克羔羊肠道中的分布表达,试验采用免疫组织化学SABC-DAB法对GFAP的表达进行了研究.结果显示,GFAP在羔羊肠道的黏膜上皮层以及黏膜固层中强表达,在固有肌层弱表达;在各个肠段均有阳性反应,且小肠段GFAP的OD值显著高于大肠段(P<0.05).GFAP是肠神经胶质细胞... 相似文献
3.
为了探索HSP70在牦牛肠道组织中的分布与变化规律,选择6头份健康成年(3~6岁)牦牛的肠道组织,采用免疫组织化学(IHC)、蛋白免疫印迹(WB)和实时荧光定量PCR(RT-qPCR)检测HSP70在牦牛十二指肠、空肠、回肠、盲肠、结肠和直肠组织中的分布与变化规律。免疫组织化学结果显示,HSP70在牦牛小肠的黏膜层、黏膜下层和肌层中均有表达,其中小肠黏膜上皮的柱状细胞呈强阳性表达,杯状细胞未见表达;小肠腺的肠腺细胞和肌层的平滑肌细胞也呈阳性表达,阳性反应主要分布于阳性细胞的细胞质和细胞核。HSP70在大肠中的定位表达与小肠相似,在大肠壁黏膜层、黏膜下层和肌层三层结构中也均有表达,其阳性反应主要位于黏膜上皮和固有层内散在分布的结缔组织细胞中,而固有层内大肠腺细胞仅在细胞核有表达,细胞质呈阴性。蛋白免疫印迹和免疫组化积分光密度值分析得出,从小肠的前端到大肠末端,HSP70的表达呈先增高后降低的趋势,其中在盲肠中的表达量达到峰值,且盲肠和结肠中HSP70的相对蛋白表达量极显著高于其他肠段(P0.01)。实时荧光定量PCR结果显示,HSP70mRNA在盲肠中的相对转录量最高,分别是十二指肠、空肠、回肠、结肠和直肠的2、1.68、1.06、1.43和1.52倍。本试验显示牦牛肠道中HSP70呈广泛性阳性反应,HSP70基因和HSP70蛋白在牦牛小肠和大肠中具有不同的表达模式,揭示了HSP70在牦牛小肠和大肠中所发挥生物学作用的异同,为HSP70在反刍动物组织中的表达和功能研究提供了资料。 相似文献
4.
通过石蜡切片和HE染色方法观察了宁都黄鸡小肠的显微结构,同时通过改良甲苯胺蓝染色的方法鉴定小肠中的甲苯胺蓝染色阳性细胞.结果显示,宁都黄鸡小肠与一般禽类小肠显微结构类似,黏膜下层缺乏十二指肠腺,黏膜固有层缺乏淋巴小结.小肠从前到后,杯状细胞逐渐增多.3段小肠的肠腺和回肠杯状细胞呈甲苯胺蓝染色阳性.小肠壁四层结构中都有肥大细胞的分布,主要分布于黏膜上皮细胞下、黏膜下层、肌层和外膜结缔组织中.肥大细胞体积较大,多数呈卵圆形,有的细胞形态不规则,甚至有突起.胞核染色呈深蓝色,胞质中含有紫红色颗粒.结果表明,宁都黄鸡小肠腺细胞和杯状细胞甲苯胺蓝染色呈阳性,说明其能够分泌有助于吸收的活性物质;小肠中具有丰富的肥大细胞,说明宁都黄鸡有较完善的肠道免疫屏障. 相似文献
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6.
斑马鱼肠道显微和超微结构的研究 总被引：2，自引：0，他引：2
应用光镜和电镜对斑马鱼肠道的组织形态和超微结构观察分析。斑马鱼肠道可分为前、中、后3段,肠壁由内向外分为黏膜层、肌层和浆膜层。测量肠道各段的绒毛高度、绒毛纵截面积、柱状上皮高度和肌层厚度,各指标从前肠到中肠和后肠呈逐渐降低趋势,前肠显著高于中肠和后肠(P<0.05),中肠和后肠间无显著差异(P>0.05)。黏膜层向肠腔内折叠形成肠绒毛(多呈分枝状、指状),分为黏膜上皮和固有层,柱状上皮细胞之间的连接复合体发达,上皮细胞间分布着杯状细胞,上皮内淋巴细胞广泛分布于肠上皮下部,而肥大细胞主要分布在后肠上皮基底膜附近。细胞水平结果表明:斑马鱼肠道执行消化吸收的主要场所在前肠,肠道黏膜免疫机能与免疫细胞位置的分布相关。 相似文献
7.
本试验应用免疫组织化学方法和显微图像分析技术,研究了2、16、30、44、58日龄岭南黄鸡外周器官中Ghrelin免疫反应阳性细胞的定位分布与发育性变化。结果表明,在鸡腺胃、肠、心、肝、脾、肺、肾、胰、脑垂体、肾上腺、胸腺、法氏囊中均可观察到Ghrelin免疫阳性反应。其阳性细胞类型包括:腺胃腺小管的内分泌细胞,肠道黏膜上皮和肠腺内的内分泌细胞,肠道黏膜下层和肌层间的神经丛,心内膜下层的蒲肯野纤维,肝血窦中的枯否氏细胞,脾的巨噬细胞和网状细胞,肺的巨噬细胞,肾脏肾小囊壁层的扁平上皮细胞和脏层的足细胞、球内系膜细胞和球旁复合体,胰腺的胰岛细胞,腺垂体的部分嗜酸性细胞和嗜碱性细胞,肾上腺的嗜铬细胞,胸腺上皮细胞、胸腺小体和巨噬细胞,法氏囊黏膜上皮和小结相关上皮内的内分泌细胞、囊小结的网状细胞、巨噬细胞、皮质髓质交界处的上皮细胞等。2~30日龄鸡,随着日龄的增长,各器官中的Ghrelin表达量增加,44、58日龄则有所下降。 相似文献
8.
家禽肠道黏膜的作用及保护 总被引：1，自引：0，他引：1
家禽肠道黏膜由黏膜上层、固有层和黏膜肌层构成。黏膜上皮为单层柱状上皮,上皮细胞之间分布有许多杯状细胞,还有银亲合细胞,特别是十二指肠前段,这种细胞更多。固有膜由含有较多细胞成分的结缔组织构成,其中有血管、神经和肠腺,有时还有弥散性淋巴组织,在局部 相似文献
9.
《中国兽医学报》2014,(12):1989-1994
为了揭示成年牦牛大肠结构和黏膜免疫相关细胞的分布规律,本研究采用光镜及透射电镜等技术,对牦牛盲肠、结肠和直肠组织结构及黏膜免疫相关细胞的分布与数量变化进行研究。结果显示,牦牛大肠壁由黏膜层、黏膜下层、肌层和浆膜构成。大肠黏膜上皮为单层柱状细胞及散在的杯状细胞,在直肠黏膜上皮中则有大量杯状细胞,黏膜上皮纹状缘不明显。固有层内含有大量肠腺和孤立淋巴小结,尤其在盲肠、结肠固有层孤立淋巴小结丰富,且生发中心明显,淋巴小结常伸入黏膜下层。大肠黏膜层平均厚度为(864.18±88.46)μm,其中结肠最厚,盲肠最薄,结肠与盲肠之间差异显著(P<0.01)。肌层分内环与外纵2层,结肠与直肠肌层发达,盲肠肌层最薄,盲肠肌层内环肌形成特殊的肌小结。上皮内淋巴细胞较多,以结肠最丰富,盲肠与结肠差异不明显,从结肠到直肠逐渐减少。盲肠有丰富的浆细胞,从盲肠、结肠至直肠依次减小。肥大细胞在结肠和盲肠均较多,在直肠则显著减少。超微结构显示,大肠黏膜上皮细胞之间有连接复合体,细胞游离面有许多排列整齐的微绒毛。上皮内淋巴细胞胞质少,胞核较大。肥大细胞胞质内有大小不等的高电子密度圆形颗粒聚集。研究表明,牦牛大肠组织结构和其他反刍动物基本相似,但肠壁肌层厚,盲肠有特殊的肌小结,盲肠和结肠黏膜层分布着丰富的孤立淋巴小结和黏膜免疫相关细胞。 相似文献
10.
动物肠道生理活动受到各级神经系统及体液的调控,特别是依赖于肠神经系统的调控作用.肠神经包括肌间神经丛和粘膜下神经丛,其各类神经元可通过多种神经递质的相互作用来调节肠道的分泌和运动.论文就脊椎动物肠道中几种主要的神经递质的分布及功能作一简要论述,以期为进一步探讨肠神经系统调节机制及脊椎动物肠道疾病的研究提供基础性资料. 相似文献
11.
ZHANG Yu-yu ZHAO Xin-lin QI Feng-hua LU Yan SHI Xiao-yu HAN Xiao-li XU Chun-sheng 《中国畜牧兽医》2017,44(5):1315-1320
The experiment was conducted to explore the distribution of glial fibrillary acidic protein (GFAP) in Yellow feather broiler,and to investigate the morphological characteristics of glial cells of chicken. The distribution of GFAP was studied by immunohistochemistry SABC-AP method. The results showed that GFAP were expressed strong positively in chicken small intestinal mucosa epithelium, intestinal gland cell cavity surface, submucosal plexus and myenteric plexus; The expressions of GFAP were positive in the mucosal lamina propria and myenteric nerve plexus around the blood vessel; In avian escherichia sticky epithelial membrane, colorectal adenocarcinoma,GFAP were expressed positively, and the expressions were strong positive in mucosa epithelium, submucosal plexus and myenteric plexus. GFAP was one of the specific marker of enteric glial cells, and the observation of distribution of GFAP in chicken intestinal tract was help for elucidating the enteric glial cells in the distribution of the intestine and providing the morphological basis for the study of chicken glial cell function. 相似文献
12.
Ganglioneuromas are complex tumors that arise in peripheral ganglia and are composed of well-differentiated neurons, nerve processes, Schwann cells, and enteric glial cells. The term ganglioneuromatosis (GN) denotes a regional or segmental proliferation of ganglioneuromatous tissue. This report describes an 8-year-old mixed breed horse with GN in a 25-cm segment of small colon. Grossly, the lesion consisted of numerous sessile to pedunculated nodules extending from the serosal surface. Histologic examination revealed the nodules to consist of fascicles of spindle-shaped cells consistent with Schwann cells, clusters of neurons, supporting enteric glial cells, and thick bands of perineurial collagen. Most of the nodules coincided with the location of the myenteric plexus and extended through the outer layer of the tunica muscularis to the serosal surface. Neuronal processes were demonstrated within the lesion with electron microscopy. With immunohistochemistry neurons were positive for neuron specific enolase (NSE) and S-100 and the Schwann cells and enteric glial cells were positive for S-100 and glial fibrillary acidic protein (GFAP). The pathogenesis of GN is poorly understood. GN, although rare, should be included in the differential diagnosis of gastrointestinal tumors in the horse. 相似文献
13.
Neurohistological and immunohistochemical examinations of the feline enteric nervous system (ENS) were performed by using antibodies against neuron-specific enolase (NSE), phosphorylated neurofilaments (PN), non-phosphorylated neurofilaments (NPN) and vasoactive intestinal peptide (VIP), whereas glial cells were investigated by using antibodies against glial fibrillary acidic protein (GFAP). The study included full-thickness biopsies of the stomach, duodenum, jejunum, ileum and colon of 11 healthy cats. In this study, immunohistochemical staining of feline ENS with antibodies to NSE, PN and NPN revealed the presence of different ganglionated and aganglionated plexus. The two ganglionated plexus were arranged in a plexus submucosus internus & externus and a plexus myentericus. Furthermore, plexus mucosus and subserosal plexus represented two aganglionated plexus. GFAP-stained cellular elements were smaller than and in close contact to enteric neurons possibly resembling astrocytes of the central nervous system. VIP is one of the major neurotransmitters of enteric inhibitory neurons, and immunoreactivity was present in all layers of the gut, especially in ganglionated plexus. This is the first report, describing feline ENS by using immunohistochemical methods. 相似文献
14.
Tissue Culture of the Enteric Nervous System from Equine Ileum 总被引：2，自引：0，他引：2
Ileal samples were harvested fresh from euthanized adult horses. The tissues were microdissected to prepare wholemount preparations for immunohistochemistry and for either explant or dissociated culture systems of the enteric nervous system. Explant culture systems were established using wholemounts of either the submucous plexus or the muscularis externa (including the myenteric plexus). Dissociated cell cultures could only be obtained from the submucous plexus. Culture systems were maintained for up to 5 days. Immunoreactivity for a neuronal marker (Pan-N) and for glial cell markers (GFAP and S100) indicated the presence of both neurons and enteric glia in the tissue culture preparations.This is the first report of equine enteric neurons being grown in tissue culture. Further refinements to the techniques will be required before this in vitro model can be used for quantitative analysis. 相似文献
15.
The equine enteric nervous system — Neuron characterization and distribution in adults and juveniles
D. L. Doxey G. T. Pearson E. M. Milne J. S. Gilmour H. K. Chisholm 《Veterinary research communications》1995,19(6):433-449
A study of myenteric and submucosal plexuses was undertaken in the jejunum and ileum of horses and ponies in which no clinical or pathological evidence of intestinal abnormality was apparent. Complete transverse sections of the intestine, stained by a modified haematoxylin and eosin method, were examined using up to 20 sequential sections per animal. Information was gathered from adult, juvenile and fetal equidae. In adults, the longitudinal muscle layers were thinner than the circular muscle layers and the ileum had thicker layers compared to the jejunum. In adults, the submucosal plexus had more neurons per section than the myenteric plexus by mean ratios of 1:3 in the jejunum and 1:1.9 in the ileum. In juveniles, the ratios were respectively 1:1.8 and 1:1.5 and in the fetus 1:2.5 and 1:1.3. The three-dimensional distribution of neurons in both plexuses varied from animal to animal and no consistent pattern was observed. Groups of neurons contained between one and 42 cells per section examined and their length in a cranio-caudal direction varied from 10 to over, 100 µm. There were few statistical differences observed between the cranial, middle and caudal portions of either the jejunum or the ileum when neuron groups or neuron numbers per section were examined in 10 adult animals.Abbreviations H&E
haematoxylin and eosin
Deceased; formerly of the Moredun Research Institute, 408, Gilmerton Road, Edinburgh, EH17 7JH, UK 相似文献
16.
Eloy Redondo ngela García Cristina Ortega Fernando J. Pea Antonio Gzquez Javier Masot 《Animal Science Journal》2020,91(1)
The histomorphological changes occurring in the Dama dama reticulum during prenatal development have been investigated. Twenty‐five Dama dama embryos were used, from the first stages of prenatal life until birth. Differentiation of the reticulum was observed at 23% gestation. By 25% gestation the reticular wall comprised three layers: an internal epithelial layer, a middle layer of pluripotential blastemic tissue and an external layer or serosa. Primary reticular crests were visible at 38% gestation. Secondary reticular crests were observed at 61% gestation. Neuroendocrine cells were detected by synaptophysin (SYP) at 35% gestation, in the lamina propria‐submucosa, tunica muscularis, and serosa. Epithelial Cytokeratin‐18 (CK‐18) cells were observed at 35% gestation extended throughout the epithelial layers. The glial cells (vimentin –VIM‐ and glial fibrillary acidic protein‐GFAP‐markers) were discerned at 25% and 43% gestation, respectively, in myenteric and submucosal plexuses, lamina propria, muscularis mucosae, tunica muscularis, and perivascular connective tissue. The neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) markers were immunodetected at 75% and 80 gestation, respectively, in the lamina propria‐submucosa, muscularis mucosae, tunica muscularis, serosa, and myenteric plexuses. The prenatal development of the fallow deer reticular mucosa evidenced a considerable precocity similar to that previously reported in goat and red deer. 相似文献
17.
The distribution of the putative motor excitatory neurotransmitter, substance P, was studied immunocytochemically in the left dorsal colon of four normal control ponies and three ponies with amitraz-induced impaction colic. Substance P-like immunoreactivity in the control ponies was observed in nerve fibres in all layers of the bowel wall and in the nerve cell bodies of the enteric ganglia. The substance P-like immunoreactivity was clearly more intense in the cell bodies of submucosal ganglia than in those of the myenteric ganglia. The internodal nerve strands of the myenteric plexus were very rich in substance P-like immunoreactivity and within the ganglia they formed dense varicose networks around the neuronal cell bodies. Nerve bundles rich in substance P-like immunoreactivity diverged inward from the myenteric plexus to contribute an abundance of varicose immunoreactive fibres to the circular muscle of the tunica muscularis. Nerve fascicles with substance P-like immunoreactivity were sparse in the longitudinal muscle except in the thickened taenial band. In the submucosa many of the nerve fibres with substance P-like immunoreactivity appeared to arise from ganglionic cell bodies. Immunoreactive fibres commonly condensed around arterial vessels in the submucosa. Fine immunoreactive nerve fascicles from the submucosal plexus also projected internally to supply the muscularis mucosae and form periglandular arrays in the lamina propria. The distribution of substance P-like immunoreactivity in the normal equine colon differed in some respects from patterns observed in large intestines of other mammals. When the colons of normal and amitraz-treated ponies were compared no differences were discerned in the distribution or intensity of substance P-like reactivity. 相似文献
18.
《Research in veterinary science》2014,96(3):837-845
The activity of the gastrointestinal tract is regulated through the activation of adrenergic receptors (ARs). Since data concerning the distribution of ARs in the horse intestine is virtually absent, we investigated the distribution of β2-AR in the horse ileum using double-immunofluorescence. The β2-AR-immunoreactivity (IR) was observed in most (95%) neurons located in submucosal plexus (SMP) and in few (8%) neurons of the myenteric plexus (MP). Tyrosine hydroxylase (TH)-IR fibers were observed close to neurons expressing β2-AR-IR. Since β2-AR is virtually expressed in most neurons located in the horse SMP and in a lower percentage of neurons in the MP, it is reasonable to retain that this adrenergic receptor could regulate the activity of both secretomotor neurons and motor neurons innervating muscle layers and blood vessels. The high density of TH-IR fibers near β2-AR-IR enteric neurons indicates that the excitability of these cells could be directly modulated by the sympathetic system. 相似文献
19.
Angela Garcia Javier Masot Antonio Franco Antonio Gazquez Eloy Redondo 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(1):35-43
Here we report the detection and distribution of synaptophysin (SPY), non-neuronal enolase (NNE), glial fibrillary acidic protein (GFAP), vimentin (VIM), neuropeptide Y (NPY), and vasoactive intestinal peptide (VIP) expression in the goat forestomach during prenatal development. A total of 140 embryos and fetuses were examined to evaluate protein expression from the first stage of prenatal life until birth. In all cases, SPY immunoreactivity was detected at 53 days gestation in the lamina propria-submucosa, tunica muscularis, serosa, and myenteric plexuses. Immunoreactivity to NNE was observed at 64 days gestation in the same locations as well as the epithelial layer. Glial cells were found at 64 days as indicated by signals corresponding to GFAP and VIM at 39 days. Positive staining for NPY and VIP was observed at 113, 75, and 95 days in the rumen, reticulum, and omasum, respectively, in the lamina propria-submucosa, tunica muscularis, and myenteric plexuses of each of these gastric compartments. These findings indicate possible preparation of the fetal goat forestomach for postnatal function. Compared to other ruminant species, neuroendocrine cells, glial cells and peptidergic innervations markers were detected earlier compared to sheep but at around the same stage as in deer. 相似文献