共查询到18条相似文献,搜索用时 171 毫秒
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为检测黑色素受体1(melanocortin receptor 1,MC1R)基因型在不同毛色猪种中的分布,研究该基因在猪毛色决定中的地位和作用,本试验使用PCR-SSCP和PCR-RFLP方法对军牧1号白猪、杜洛克猪、西藏小型猪和大白猪的MC1R基因型进行了检测。结果显示,长白猪、大白猪存在nt894insCC和G1197A突变;杜洛克猪存在G668C、C1318T和G1554A突变;西藏小型猪存在C1318T和G1554A突变,而在nt894insCC位点则表现出其他基因型。通过对4个猪种的MC1R基因型的检测,为研究MC1R基因对毛色的作用机理奠定了基础。 相似文献
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MCR蛋白在不同毛色太行山羊皮肤组织中的表达定位 《畜牧与饲料科学》2016,37(8):5-5
MC1R基因是调节哺乳动物毛色形成的重要候选基因之一,编码黑色素皮质素受体1(MC1R),在哺乳动物黑色素的代谢中发挥重要作用。分别选取纯黑色、纯白色、黄褐色和黑斑白色的太行山羊皮肤组织,通过HE染色技术,观察太行山羊的皮肤组织结构;利用免疫组化技术,对MC1R蛋白在4种毛色太行山羊皮肤组织中的表达情况进行研究。结果表明,太行山羊皮肤由表皮、真皮、皮下结缔组织3部分组成;MC1R蛋白在4种毛色太行山羊的表皮、真皮中均没有表达,但在纯黑、黄褐和黑斑白色太行山羊毛囊中有表达,且主要集中在毛囊外根鞘处。研究结果为太行山羊进一步的提纯选育工作提供了一定的依据。 相似文献
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为了将毛色遗传标记作为湘西黄牛杂交组合选择、纯种鉴别、保种选育的科学理论依据,应用PCR-RFLP技术,研究了湘西黄牛黑素皮质素受体1(Melanocortin 1 Receptor,MC1R)基因对其黑毛色和红毛色表型的影响机制.结果表明:湘西黄牛中MC1R基因有三个等位基因 (ED 、E+、e );隐性的e基因在310处缺失一个碱基G 造成移码突变,在468处提前终止翻译,翻译出一个缩短了的失去功能的MC1R蛋白,表现或强或弱的红毛色;E+所对应的是野生型毛色,如黄色、灰色等;湘西黄牛中黄色个体优势等位基因为E,而红色个体优势等位基因为e,e相对E是隐性基因,只有在e等位基因纯合状态下,才表现为红色毛色; E+/e也表现为红色,可能湘西黄牛红色和黄色表型除了有MC1R基因控制外,还有其它基因参与作用. 相似文献
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ASIP、MC1R和TYR是与动物毛色性状相关的重要候选基因,为了探究ASIP、MC1R、TYR基因多态性及其与贵州白水牛白色被毛之间的关系。本研究以贵州白水牛(20头)和其他毛色水牛(60头)为试验材料,针对3个基因的外显子和部分非编码序列设计引物,利用DNA池作为模板进行扩增测序,对ASIP、MC1R和TYR基因多态性与贵州白水牛被毛之间的关系做了初步探讨。结果发现,在这3个基因中均没有发现特异性的插入/缺失突变,在贵州白水牛ASIP基因的启动子中发现了突变位点:Promoter-A147T和Promoter-A235G;在MC1R基因中筛选到1个错义突变exon1-T843C,TYR基因中筛选出2个错义突变exon1-G826A、exon5-T70C,以及1个同义突变exon3-A83G。这3个基因的SNP位点在扩大样本验证时发现不具有毛色特异性,研究初步表明,ASIP、MC1R、TYR基因的多态性位点不能解释贵州白水牛毛色的特殊变异,为以后对贵州白水牛的毛色的进一步研究奠定了基础。 相似文献
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为了探讨蒙古马的毛色与黑素细胞激素受体1(MC1R)基因的相关性,试验采用PCR-RFLP技术对蒙古马5个类群及三河马和纯血马的MC1R基因进行了多态性检测,并对多态位点与毛色性状之间的相关性进行分析.结果表明:蒙古马MC1R基因在901 bp处发生碱基错义突变(C→T),该突变导致出现EE、Ee及ee 3种基因型.群... 相似文献
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Research Advances on MC1R Gene 总被引:1,自引:0,他引:1
MC1R gene, as one of the important candidate genes for controlling coat color, not only plays an important role in the process of melanin synthesis, but also participates in the formation of coat color and also has different effects on human and animal diseases, growth traits and behaviors. The study of MC1R gene has attracted the attention of scholars.In order to deepen the understanding of MC1R gene, the structure, function, mechanism, gene locus, polymorphism analysis and research progress of human and animal of MC1R gene were reviewed and new ideas were put forward in this review. 相似文献
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在欧洲马品种中,黑色素皮质激素受体1(melanocortin receptor 1,MC1R)基因第248位碱基有C/T多态性,纯合的T248位点决定欧洲马的栗毛色。针对MC1R基因的248位点设计了2对特异性引物,采用等位基因特异性PCR技术,研究3个中国马品种MC1R基因型与栗色毛之间的关系。经扩增获得两种DNA片段,克隆测序后证明,扩增片段确为MC1R基因,两种DNA片段序列在基因的248位点的确呈现C/T多态性,但检测126份贵州矮马、西南马和新疆伊犁马血液样本,全部为杂合基因型(Ee),其中包括栗毛、黑毛、骝毛3种单毛色及3种复毛色。这些研究结果提示,MC1R基因中248位点的多态性与国内3个马品种的栗色毛之间没有直接的相关性。 相似文献
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牛是重要的家畜之一,在役用、肉用和乳用等方面对社会有着重要的贡献。通过鉴定牛经济性状相关基因及其变异有助于提高育种效率,增加经济效益。随着现代生物技术的发展,对牛经济性状主效基因的挖掘变得更加准确。已鉴定与牛生长发育相关的PLAG1基因,与繁殖相关的GDF9基因,与肉质相关的CAST基因,与胴体相关的MSTN基因,与毛色相关的MC1R基因,与泌乳相关的DGAT1基因等。针对牛不同性状进行合理的分子选育对牛业发展有着重要的经济价值和意义。本文对近年来黄牛生长发育性状、繁殖性状、肉质性状、胴体性状、毛色性状和泌乳性状主效基因研究进展进行综述,为今后黄牛遗传育种研究提供参考依据。 相似文献
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MC1R基因与朝鲜鹌鹑羽色关系的研究 总被引:1,自引:0,他引:1
黑素皮质素受体1(MC1R)又称促黑素细胞激素受体(MSH-R),由毛色扩展位点编码而成,对动物体色的形成起重要作用,本研究分析了MC1R基因突变与朝鲜鹌鹑羽色之间的关系。通过混合样品DNA测序方法寻找MC1R基因的突变位点,采用PCR-SSCP的方法对突变位点在4种羽色鹌鹑(栗羽、黄羽、白羽、黑羽)群体中的分布情况进行了研究,以揭示MC1R基因与鹌鹑羽色性状之间的关系。结果表明,在鹌鹑MC1R基因上存在一个A/G突变位点,导致编码蛋白发生Ile58Val突变,通过PCR-SSCP分析发现,该突变位点在4种羽色鹌鹑群体间没有显著差异(P>0.05)。本研究没有发现与日本鹌鹑报道相同的Glu92Lys突变位点,表明朝鲜鹌鹑的黑羽突变与报道的日本鹌鹑黑羽突变的机制不同,而且朝鲜鹌鹑的黑羽突变可能还与其它基因突变有关。 相似文献
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Oguro-Okano M Honda M Yamazaki K Okano K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(7):853-858
To identify factors that control coat color in Akita-inu dogs, we sequenced all the exons of the melanocortin 1 receptor (MC1R), β-defensin103 (CBD103) and agouti signaling protein (ASIP) genes of dogs with four distinct coat colors, namely, brindle, sesame, red and white. Then we examined correlations among specific alleles and coat color. In the case of the MC1R gene, all white dogs were homozygous for a nonsense mutation, R306ter, while brindle, sesame, and red dogs had at least one R306 allele. In the case of the CBD103 gene, all brindle dogs were heterozygous for the G23del mutation (deletion of codon 23, encoding glycine), while all sesame and red dogs were homozygous for G23. In the case of the ASIP gene, all dogs, regardless of coat color, had at least one S82 H83 allele. A missense mutation in the ASIP gene, P87L, was identified for the first time in some Akita-inu dogs but was not associated with any specific coloration. Our results indicate that the 2 key mutations, R306ter in the MC1R gene and G23del in the CBD103 gene, are associated with the phenotypic discriminations among brindle, red/sesame, and white coats, while no mutation that might potentially be associated with the discrimination of a sesame coat from a red coat is present in the coding sequences of these three genes. 相似文献
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QIN Ke HUANG Xiang ZENG Jian-hua WANG Chen SUN Guan-jie SONG De-qing CHEN Yao-sheng LIU Xiao-hong HE Zu-yong 《中国畜牧兽医》2017,44(3):773-781
In order to assess the purity of Luchuan pig populations, four South Chinese local pig breeds including Putian Black pig, Yuedong Black pig, Dahuabai pig, Bama miniature pig and three foreign pig breeds including Duroc pig, Yorkshire pig and Landrace pig were studied as controls by sequencing of PCR products, MC1R and KIT genotypes in 56 Luchuan pigs were analyzed in this study. Sequencing results indicated that a splicing mutation (G>A) was presented in the first base in intron 17 of KIT gene in both Yorkshire pig and Landrace pig, in contrast, the wildtype GG of KIT gene was presented in Luchuan pig, four south Chinese local pig breeds and Duroc pig.Compared with Hainan wild boar, South Chinese local pig breeds had two missense mutations 95Val > Met and 102Leu > Pro in the coding region of MC1R gene;Compared with Yorkshire pig, Landrace pig and Duroc pig, South Chinese local pig breeds had 5 to 6 SNPs in MC1R gene 5'UTR, and in addtion, an A base deletion in MC1R gene 3'UTR. Furthermore, we found one litter of Luchuan pig with abnormal coat color.The results showed that the presentation of two distinct MC1R genotypes ED1 and Ep in both litters and the sow,but only ED1 in the boar. Considering Ep was derived from Pietrain pig, we preliminarily considered that the genome of the sow might be infiltrated with foreign pig breeds. In summary, we detected the genotypes of the coat color genes KIT and MC1R in eight pig breeds, confirmed the molecular differences of coat color between Chinese local pig breeds and foreign pig breeds, which could be useful for the further investigation of the molecular mechanism of pig coat color. 相似文献
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为了检测陆川猪种群的纯度,本研究以4个华南地方猪种(莆田黑猪、粤东黑猪、大花白猪、巴马香猪)和3个外来猪种(杜洛克猪、大白猪、长白猪)作为对照,利用PCR测序法对陆川猪群体56个样本的MC1R和KIT基因进行基因型鉴定。测序结果表明,大白猪和长白猪KIT基因内含子17的第1个碱基发生G>A剪接突变,而陆川猪和4个华南地方猪种,以及杜洛克猪中KIT基因型一样,均为野生型的GG基因型;MC1R基因编码区分析表明,以海南野猪MC1R基因型作为参考序列,陆川猪和4个华南地方猪种在MC1R基因编码区存在95Val > Met和102Leu > Pro两个错义突变;MC1R基因非编码区分析表明,与大白猪、长白猪和杜洛克猪相比,陆川猪和4个华南地方猪种MC1R基因的5'UTR分别存在5~6个SNPs,3'UTR存在1个A碱基的缺失。另外,试验对一窝毛色异常的陆川猪仔猪及其亲本的KIT和MC1R基因进行测序分析,结果表明,仔猪及其亲本的KIT基因型均为GG野生型,仔猪和母本的MC1R基因均存在ED1和Ep两种不同的基因型,父本的MC1R基因型为ED1,因Ep对应于皮特兰猪的斑块表型,所以初步判定母本渗入了外来猪种的血统。本研究通过检测陆川猪等8个猪种的毛色相关基因KIT和MC1R基因的基因型,证实了中外猪种间在毛色遗传上的分子差异,为今后深入研究猪毛色遗传的分子调控机理提供参考。 相似文献