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1.
对菊黄东方鲀、红鳍东方鲀及其杂交后代的早期形态特征和生长速度进行了比较。在全长5 cm和10 cm时,形态上的主要差异是:(1) 菊黄东方鲀的尾鳍为浅黄色,无黑杂色;红鳍东方鲀的尾鳍为黑色,其杂交后代与菊黄东方鲀相似,尾鳍浅黄色,略带黑色;(2) 菊黄东方鲀背部皮刺无或极少超出侧线,超出部分与体背黑斑不相交;红鳍东方鲀背部皮刺超出侧线,超出部分与黑斑相交,向下延伸至腹部,其杂交后代背部皮刺超出侧线,与体背黑斑部分相交,向下未能延伸至腹部;(3) 菊黄东方鲀背部皮刺向前延伸至两眼连接线,红鳍东方鲀向前延伸至两鼻孔连接线,其杂交后代超过两眼连接线而未达鼻孔连接线。以尾鳍颜色和皮刺的分布特征可以区分幼鱼阶段的菊黄东方鲀、红鳍东方鲀及其杂交后代。在体长为5 cm及10 cm时分别选取了2个和4个框架参数建立判别函数,判别菊黄东方鲀、红鳍东方鲀及其杂交种的准确率达到96.8%和100%。经过110 d的饲养,红鳍东方鲀、杂交东方鲀以及菊黄东方鲀的平均体长分别达到(110.24±3.78)、(101.16±6.56)和(82.92±4.29) mm,体质量分别为(35.68±5.04)、(33.00±6.24)和(20.99±3.00) g,无论体长还是体质量,都是红鳍东方鲀>杂交东方鲀>菊黄东方鲀,差异极显著。研究表明,菊黄东方鲀♀与红鳍东方鲀♂杂交,其后代早期形态与母本菊黄东方鲀相似,生长比红鳍东方鲀慢,而比菊黄东方鲀快,具有显著的经济杂交价值。 相似文献
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利用封闭呼吸室对同一日龄、不同规格的红鳍东方鲀、菊黄东方鲀和菊黄东方鲀(♀)×红鳍东方鲀(♂)杂交F1代幼鱼进行耗氧率和临界窒息点的研究。试验结果表明,水温14.8～15.6℃时,体质量(37.24±3.64)g的红鳍东方鲀幼鱼耗氧率为(0.3385±0.0161)mg/(g.h),体质量(14.45±1.08)g的菊黄东方鲀幼鱼耗氧率为(0.2327±0.0241)mg/(g.h),体质量(27.96±1.38)g的杂交F1代东方鲀幼鱼耗氧率为(0.2282±0.0219)mg/(g.h);同一日龄不同规格红鳍东方鲀、菊黄东方鲀和杂交东方鲀幼鱼的耗氧量分别为(12.5243±0.6720)、(3.3544±0.2975)、(5.8469±0.9537)mg/(h.尾);3种东方鲀的耗氧率呈明显的昼夜节律,白天平均耗氧率显著高于夜晚。水温为14.8～15.6℃时,红鳍东方鲀、菊黄东方鲀及杂交东方鲀幼鱼的临界窒息点分别为0.665、0.882mg/L和0.774mg/L。 相似文献
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1 发生 在我国,红鳍东方鲀盾纤虫病最早于2000年初春由笔者确认于大连1家企业水槽越冬养殖的1龄红鳍东方鲀(杜佳垠,2000)。尔后,笔者在大连4家企业先后确认10例红鳍东方鲀盾纤虫病。其中,包括2002年初夏1家企业水槽培育1月龄红鳍东方鲀稚鱼盾纤虫病、链球菌病并发症;2002年晚春1家企业水槽越冬养殖1龄和2龄红鳍东方鲀盾纤虫病、冈本异沟吸虫病、链球菌病并发症;2002年初冬1家企业水槽越冬养殖当年红鳍东方鲀盾纤虫病、链球菌病并发症和2年红鳍东方鲀盾纤虫 相似文献
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红鳍东方鲀(Takifugu rubripes)为底栖肉食性的沿岸种类。因其性凶猛、个体大、生长陕、味鲜美,素享"鱼类之王"之美誉。1990年黄海水产研究所与烟台海带育苗场和烟台市水产研究所合作,进行红鳍东方鲀工厂化育苗首次在国内获得成功以后,我国北方和南方相继进行红鳍东方鲀的工厂化养殖,红鳍东方鲀的人工育苗技术不断改 相似文献
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为了丰富海参池塘养殖的混养种类,实验对刺参和红鳍东方鲀的生态混养效果进行了研究。结果显示,经过100 d的混养实验,红鳍东方鲀平均日增重率为1.07 g/d,特定生长率为4.06%/d,混养组与单养组红鳍东方鲀没有显著差异;但混养条件下刺参的生长状况显著优于单养条件下刺参的生长状况。与红鳍东方鲀混养组刺参平均日增重率为(0.11±0.04)g/d,特定生长率为(0.67±0.20)%/d。单养组刺参平均日增重率为(0.04±0.02)g/d,特定生长率为(0.35±0.19)%/d。研究表明,刺参池塘混养红鳍东方鲀模式下,红鳍东方鲀在正常快速生长的同时,可以有效促进刺参的生长,研究结果可以为刺参池塘的生态复合养殖提供参考依据。 相似文献
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1生产现状 在日本,近20年来,作为名贵鱼类,红鳍东方鲀一直列为重要养殖对象.日本红鳍东方鲀养殖产量已于1993年达达4 427 t,与近年一直维持在5000t左右.日本红鳍东方鲀养殖主要集中于九州和四国地区,养殖红鳍东方鲀主要产地为熊本县、长崎县、鹿儿岛县和爱媛县.在日本,红鳍东方鲀养殖一向为海面网箱养殖. 相似文献
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根据红鳍东方鲀生长激素基因全序列,设计13对引物,结合单链构象多态性(SSCP)技术和克隆测序技术对野生红鳍东方鲀、养殖红鳍东方鲀和养殖暗纹东方鲀群体进行了遗传多样性分析.结果表明:东方鲀整个生长激素基因的6个外显子中没有多态性,在其内含子2、内含子5以及3′侧翼区发现有多处序列变异位点,包括碱基的缺失、插入和一些单核苷酸突变.这些遗传多态性的存在为从分子水平上对东方鲀的鉴定提供了一定的依据.群体遗传结构分析结果表明,红鳍东方鲀养殖群体的一些等位基因丧失,造成其遗传多样性显著下降,应及早加强对其种质的保护. 相似文献
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ABSTRACT: Rearing experiments were conducted to investigate the essential fatty acid requirements in the early developmental stages of river puffer Takifugu obscurus and tiger puffer T. rubripes using two n-3 series unsaturated fatty acids, α-linolenic acid (18:3n-3, α-LNA) and docosahexaenoic acid (22:6n-3, DHA), under salinity of 30 and 18.5–20.3°C. River and tiger puffer larvae used in this study were 15 and 14 days old after hatching, and their average body weights were 30.1 and 20.8 mg, respectively. The results on fatty acid requirements of these two species were evaluated from fish growth, survival, fatty acid composition of the fish body and activity test results. The DHA groups of both river and tiger puffer exhibited better survival and weight gain. However, there was no difference in the mean final body weights of river puffer between two dietary groups. Also, the DHA group of tiger puffer showed better results in the recovery test from anesthetic condition than that obtained in the LNA group. In an examination of the fatty acid compositions of the whole body, the LNA group containing no dietary DHA resulted in 0.5% DHA in tiger puffer and 1.1% DHA in river puffer . These results suggest that α-LNA from Artemia converted to eicosapentaenoic acid (20:5n-3, EPA) and to DHA successively by their fatty acid metabolism. Symptoms following essential fatty acid deficiency were not observed in any experimental groups. As river puffer did not represent a significant difference in the dietary effects between α-LNA and DHA treatment groups, its essential fatty acid requirement was assumed to be somewhat closer to that of the freshwater fishes in comparison with that of marine fishes, including tiger puffer. 相似文献
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异沟虫是红鳍东方鲀常见寄生虫之一,对红鳍东方鲀养殖业危害极大。为有效防治异沟虫病,在观察异沟虫虫卵孵化规律的基础上,研究评价福尔马林药浴对异沟虫的杀灭效果及其对红鳍东方鲀的安全性,形成红鳍东方鲀异沟虫病的防治方案。试验结果发现,异沟虫虫卵的孵化过程大致可分为孵化初期、孵化中期、孵化后期和破卵4个阶段,在17~19 ℃条件下,异沟虫虫卵5.5 d孵化出幼虫,8 d孵化结束;不同大小的异沟虫对福尔马林的耐受性有明显差异,当福尔马林剂量为600 mL/m 3 时,浸浴1 h可杀灭全部异沟虫幼虫(体长≤1 cm),但对异沟虫成虫(体长>1 cm)几乎无效;在600 mL/m 3 福尔马林浸浴条件下,红鳍东方鲀在浸浴后第5 h开始出现游动异常、呼吸困难等现象,在浸浴后第6 h出现死亡;而在650、700 mL/m 3 福尔马林浸浴条件下,红鳍东方鲀分别在浸浴后第5.5 h和4 h出现死亡。综上,体质量约为20 g的红鳍东方鲀异沟虫病的建议防治方案为600 mL/m 3 福尔马林溶液浸浴1 h,8 d后重复药浴1次。 相似文献
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双斑东方鲀全人工繁殖试验 总被引：2，自引：0，他引：2
对野生双斑东方鲀(Takifugu bimaculatus)人工繁殖的子一代进行人工培育,雌鱼须经三年性腺成熟,成熟系数为17.55%,雄鱼性腺成熟为两年。产卵前一个月,雌亲鱼经强化培育、催熟后,成熟系数可达到25.00%。对7组亲鱼注射LRH-A_2激素,共获鱼苗1.2048×10~6尾,其中全人工繁殖鱼苗9.592×10~5尾,对照组获鱼苗2.456×10~5尾。并就双斑东方鲀性成熟年龄等进行讨论。 相似文献
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Masashi Ando Yoko Nakagishi Keiko Yoshida Masashi Nakao Takayuki Nakagawa Yasuo Makinodan Yasuyuki Tsukamasa Ken-Ichi Kawasaki 《Fisheries Science》2006,72(5):1104-1108
Pyridinoline (Pyr), one of the mature crosslinks of collagen, was determined in muscular collagen of three species of fish.
The amounts of muscular Pyr in red sea bream, yellowtail, and tiger puffer were 3.4, 8.8, and 50.3 mmol/mol collagen, respectively,
indicating that the Pyr concentration in muscular collagen differs greatly among fish species. The Pyr concentration in tiger
puffer muscular collagen was the greatest, but it was only one-fourth that in rabbit muscle. As in mammalian skin collagen,
Pyr was not detected in skin collagen of red sea bream and yellowtail. However, tiger puffer skin contained Pyr (3.75 mmol/mol
collagen). The presence of Pyr would have a relationship to some features of tiger puffer skin, such as mechanical strength
and thickness. Pyr concentrations in acid-soluble collagen (ASC), pepsin-solubilized collagen (PSC), and insoluble collagen
(ISC) in muscles of three species of fish were determined. Pyr was found in ISC > PSC > ASC, from the highest to the lowest
concentration, and the concentration in ISC was 45–200 times that in ASC. Therefore, Pyr crosslinks that are formed between
collagen molecules would have a close relationship to collagen solubility. 相似文献
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Calcium Requirement of Tiger Puffer Fed a Semi-Purified Diet 总被引：1，自引：0，他引：1
Juvenile tiger puffer (Takifugu rubripes) were fed semi-purified diets with graded levels (0–0.40%) of calcium (Ca) supplements for 8 weeks at a temperature of 23.0–25.5 °C. At the end of the feeding experiment, average final body weight increased with increasing dietary Ca levels. A diet without Ca supplement resulted in poor average final body weight and 0.05% dietary Ca supplement could not significantly improve the growth. However, supplemental Ca levels of 0.10–0.40% significantly increased growth. Dietary Ca supplements of 0.10% or more also improved the feed efficiency in tiger puffer. Ca and phosphorus contents of the bone were independent of dietary Ca supplements. However, 0.40% Ca in the diet decreased the zinc and manganese contents of bone. Results indicated that supplemental Ca levels of 0.10–0.20% in a semi-purified diet were required to maintain normal growth, feed utilization and bone mineralization of tiger puffer. 相似文献
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N.J. Duncan N. Garcia-Aguilar G. Rodríguez-M. de O. M. Bernadet C. Martinez-Chavez C. Komar P. Estañol A. Garcia-Gasca 《Fish physiology and biochemistry》2003,28(1-4):505-506
Few mature female bullseye puffer have been observed to spawn in captivity. The ovary appears to develop to late vitellogenesis, but not to complete final oocyte maturation (FOM). Luteinizing hormone releasing hormone analogue (LHRHa) treatment induced the spawning of good quality eggs in both wild and captive brood fish. 相似文献
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Tiger puffer, Takifugu rubripes, juveniles were fed with four semi-purified experimental diets containing 0.2% Ca from Ca-lactate (diet 1), no supplemental Ca (diet 2) and 0.2% and 2.5% Ca from tricalcium phosphate, TCP (diets 3 and 4), respectively. After a 10 week rearing period, growth and feed utilization were significantly lower in the fish group fed on diet 2 than in the fish group fed on diet 1. Fish groups fed on diets 3 and 4 also showed poor growth performances compared with group 1. It appears that Ca intake from seawater is not sufficient for the normal growth of tiger puffer. Furthermore, Ca in dietary TCP appeared to be unavailable to this species. Dietary TCP strongly inhibited the bone mineralization of Zn and Mn. The findings indicate that easily digestible Ca supplementation is indispensable in a diet of tiger puffer for normal growth, feed utilization and bone mineralization. © Rapid Science Ltd. 1998 相似文献
19.
Dietary astaxanthin differentially affected the lipid accumulation in the liver and muscle of the marine teleost,tiger puffer Takifugu rubripes 
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下载免费PDF全文 Zhangbin Liao Houguo Xu Yuliang Wei Qinggong Zhang Mengqing Liang 《Aquaculture Research》2018,49(10):3421-3433
The lipid‐regulating effects of astaxanthin (ASTX) have been widely reported in terrestrial animals. However, little relevant information has been available in fish although ASTX has been used as exogenous pigment and antioxidant in fish feed. A 74‐day feeding study was conducted to investigate the effects of dietary ASTX on lipid accumulation in the marine teleost tiger puffer. Four experimental diets differing only in ASTX supplementation, that is, 0 (control), 50 (ASTX50), 100 (ASTX100) and 500 (ASTX500) mg kg‐1, were randomly assigned to 12 tanks of juvenile tiger puffer. Compared to control, the liver lipid content in group ASTX50 was significantly higher, while those in groups ASTX100 and ASTX500 were lower. The muscle lipid contents in group ASTX500 were significantly higher compared to control. Group ASTX50 had the best growth performances, while diet ASTX500 seemed to have adverse effects. In the liver, compared to control, groups ASTX50 and ASTX100 showed significantly lower mRNA expressions of genes related to triglycerol synthesis and fatty acid synthesis, transport and uptake, but higher expressions of genes related to β‐oxidation and monoglycerol hydrolysis. In the muscle, compared to control, ASTX100 showed higher expressions of genes related to β‐oxidation. ASTX50 resulted in higher contents of saturated and monounsaturated fatty acids but lower contents of n‐3 polyunsaturated fatty acids in fish. In conclusion, astaxanthin in diets for tiger puffer differentially regulated the lipid accumulation in the liver and muscle, both in dose‐dependent manners. Excess dietary astaxanthin (500 mg/kg) had adverse effects on tiger puffer. 相似文献
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Yong‐Hai Shi Gen‐Yu Zhang Ya‐Zhu Zhu Jian‐Zhong Liu Wei‐Ling Zang 《Aquaculture Research》2010,41(12):1741-1747
Tawny puffer Takifugu flavidus is a species found in China considered to have potential for aquaculture. Experiments were conducted to determine the optimal temperature for its incubation and larval culture. Fertilized eggs collected from cultured broodstocks that were induced to ovulate with a [d ‐Ala6‐Pro9‐Net]‐luteinizing hormone‐releasing hormone analogue were inseminated. The effect of temperature (19, 20, 23, 26 and 29 °C) on the hatch rate, incubation period, viability of 24 h post‐hatch larvae and total mortality rate was assessed. The effect of temperature (20, 23, 26 and 29 °C) on the growth and survival of larvae from 3 to 19 days after hatching (dah) was also assessed. The results showed that the optimal temperature for successful development of fertilized eggs ranged from 23 to 26 °C, and the highest hatch rate, the optimal viability of 24 h post‐hatch larvae and the lowest total mortality rate were all predicted using quadratic equations. The relationship between temperature and the incubation period of tawny puffer eggs was determined using the effective degree‐day model. The temperature at developmental zero (t0) was 11.34 °C, and the sum of effective degree‐days (k) was 52.356. The survival rate of tawny puffer larvae at 20 °C was significantly lower than among 23, 26 and 29 °C, whereas the survival rate was not significantly different from that at 23, 26 and 29 °C. The larval growth rate increased rapidly as the temperature increased, showing a linear relationship in the range of temperatures investigated. The optimal temperature for larval culture ranged from 23 to 29 °C. 相似文献