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1.
Zhuangbiao Zhang Xiaoyun He Qiuyue Liu Jishun Tang Ran Di Mingxing Chu 《Reproduction in domestic animals》2020,55(9):1145-1153
TGF-β induced factor homeobox 1 (TGIF1) and splicing factor 1 (SF1) are important for mammalian reproduction; however, the effects of these genes on litter size in sheep remain unexplored. In this study, we genotyped 768 ewes from seven sheep breeds at two loci: g.37871539C>T, a synonymous mutation of TGIF1; and g.42314637T>C, a 3′UTR variant of SF1. Our analysis of polymorphism revealed only two genotypes at locus g.37871539C>T in TGIF1, with most sheep populations being moderately polymorphic (0.25 < PIC < 0.5) at this site. In contrast, most breeds exhibited low polymorphism (PIC ≤0.25) at the SF1 locus g.42314637T>C. The association analysis revealed that a synonymous mutation at g.37871539C>T in TGIF1 was highly associated with litter size in Small Tail Han sheep, in which it causes a significant decrease in litter size. Conversely, while the SF1 3′UTR variant g.42314637T>C was also highly associated with litter size in sheep, it causes a significant increase in the number of litter size. Combined, these data provide valuable information regarding candidate genetic markers for sheep breeding programs. 相似文献
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Yuanyuan Gao Qi Hao Ming Cang Jianguo Wang Haiquan Yu Yongbin Liu Wenguang Zhang Bin Tong 《Reproduction in domestic animals》2021,56(12):1562-1571
Prolificacy is an important trait of animals, specifically for sheep. The Bone morphogenetic protein receptor 1B (BMPR1B) is a major gene affecting the litter size of many sheep breeds. The well-known FecB mutation (Q249R) was associated fully with the hyper prolific phenotype of Booroola Merino. However, the identification of variation in all exonic regions of BMPR1B was rare. In this study, we sequenced all exonic regions of BMPR1B gene of Mongolia sheep breed, and ten novel variants were detected by direct sequencing. Among them, the litter size of the Mongolia ewes with the CC genotype was significantly higher (0.34 additional lambs, p < .05) than those with the TT genotype of the g.29346567C>T single nucleotide polymorphism (SNP). The litter size of the Mongolia ewes with the TT genotype was significantly higher (0.19 additional lambs, p < .05 and .31 additional lambs, p < .01, respectively) than those with the GT and GG genotypes of the c.1470G>T SNP. The silent c.1470G>T mutation is predicted to increase the stability of the mRNA secondary structure through reducing minimum free energy and is predicted to change the mRNA secondary structure of BMPR1B. Our findings may give potentially useful genetic markers for increasing litter size in sheep. 相似文献
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选择小尾寒羊等5个绵羊群体为研究对象,采用PCR-SSCP和克隆测序等分析方法,检测促卵泡素β(FSHβ)基因CDS区的单核苷酸多态性,并分析其对小尾寒羊产羔数的遗传效应。结果表明:FSHβ基因CDS区第2外显子存在多态性,在5个绵羊群体中都表现出AA、AB和BB3种基因型。BB型第147位发生T→C的单碱基同义突变。高产羔数的小尾寒羊与甘肃高山细毛羊、特克塞尔羊、陶蒙杂种羊之间的基因型分布差异明显(P0.01)。5个绵羊群体均处于中度多态(0.25PIC0.5)。固定效应分析结果表明,场-年-季对小尾寒羊产羔数无影响(P0.05),胎次和基因型均影响产羔数(P0.05)。AA型小尾寒羊产羔数最小二乘均值比AB型的多0.77只(P0.05),比BB型的多0.74只(P0.05)。研究表明,FSHβ基因可能是控制小尾寒羊产羔数的一个主效基因或与之存在紧密连锁的一个分子遗传标记。 相似文献
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试验旨在研究绵羊催乳素受体(Prolactin receptor,PRLR)基因g.38976954C>A位点多态性和可卡因-苯丙胺调控转录肽(CART prepropeptide,CARTPT)基因g.9856267C>A、g.9854141G>C位点多态性与绵羊产羔数之间的关系,为绵羊多羔新品系的选育和高繁殖力机理研究提供参考。采用重测序和Sequenom MassARRAY?誖SNP技术对常年发情(小尾寒羊、湖羊、策勒黑羊)和季节性发情(草原型藏羊、苏尼特羊、滩羊、萨福克羊)的不同绵羊品种PRLR、CARTPT基因进行检测分型,然后与小尾寒羊产羔数关联分析。结果表明:g.38976954C>A位点有CC、CA和AA三种基因型,该位点基因型频率和等位基因频率在常年发情、季节性发情绵羊品种间差异不显著(P>0.05);g.9856267C>A位点存在CC、CA和AA三种基因型,g.9854141G>C位点存在GG、GC和CC三种基因型,这2个位点的基因型频率和等位基因频率在常年发情、季节性发情绵羊品种间差异均极显著(P<0.01)。群体遗传学分析结果表明,g.38976954C>A位点在小尾寒羊、草原型藏羊、滩羊和策勒黑羊中均表现为低度多态(PIC<0.25),在苏尼特羊、湖羊和萨福克羊中均表现为中度多态(0.25A位点在7个绵羊品种中均表现为低度多态(PIC<0.25);g.9854141G>C位点在小尾寒羊、苏尼特羊、草原型藏羊、滩羊、湖羊和策勒黑羊中均表现为低度多态(PIC<0.25),在萨福克羊中表现为中度多态(0.25A位点在7个绵羊品种中均处于哈代-温伯格不平衡状态(P<0.05);g.9856267C>A位点在草原型藏羊和湖羊中处于哈代-温伯格不平衡状态(P<0.05);g.9854141G>C位点在7个绵羊品种中均处于哈代-温伯格平衡状态(P>0.05)。关联分析表明,PRLR基因g.38976954C>A位点不同基因型与小尾寒羊各胎产羔数之间无显著关联(P>0.05),该位点不适用于小尾寒羊多羔性状选育;CARTPT基因g.9856267C>A和g.9854141G>C位点不同基因型与小尾寒羊各胎产羔数之间无显著关联(P>0.05),但这2个位点不同基因型各胎产羔数相差较大,推测该位点可能对产羔有影响。 相似文献
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Hamed Amirpour Najafabadi Majid Khansefid Ghassan Ghaith Mahmoud Huitong Zhou Jon G. H. Hickford 《Reproduction in domestic animals》2020,55(11):1585-1591
Having the ability to control litter size is important for sheep farmers and breeders worldwide. However, making genetic gain in key livestock traits like reproductive performance needs typically a lot of time, and both the fecundity and fertility traits have a great economic importance. Attention has therefore turned to better understanding the genes that control reproductive performance. Of these genes, research has focussed on the growth differentiation growth factor 9 (GDF9) gene (GDF9). In this study, a PCR-single strand conformation polymorphism (PCR-SSCP) approach was used to investigate variation in this gene in separate groups of purebred Finnish Landrace sheep, Finnish Landrace × Texel-cross sheep and composite sheep of undefined breed background, but based on New Zealand Romney-type genetics. Three GDF9 variants (named A, B and C) were found, and upon DNA sequencing, the nucleotide substitutions c.978A>G, c.994G>A and c.1111G>A were revealed. The frequency of variant A (containing nucleotides c.978A, c.994G and c.1111G) in the Finnish Landrace, Finnish Landrace × Texel-cross and composite sheep was 0.86, 0.78 and 0.76, respectively. In these three sheep groups, the frequency of B (defined by the presence of nucleotides c.978G and c.994A) was 0.01, 0.03 and 0.23 and for C (containing c.1111A) was 0.13, 0.18 and 0.01, respectively. An animal model was used to estimate the additive effect of fertility data for Finnish Landrace × Texel-cross sheep and revealed an association between litter size and the c.1111G>A variation (p = .036), but this was not observed for the Finnish Landrace sheep (p = .27) or the composite sheep (p = .17). When all the sheep were analysed together, the presence of c.1111A was associated (p < .05) with increased litter size, when compared to ewes that had c.1111G. Litter size did not differ between sheep with and without c.994A in all three groups of sheep investigated. This study suggests that c.1111A could be a useful genetic marker for improving fecundity in New Zealand sheep breeds and that it could be introgressed into other breeds, but analysis of more sheep will be required to confirm the associations that have been observed here. 相似文献
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The effects of parity and litter size on maternal behavior of Small Tail Han sheep was investigated at Linyi University, China. Sixty‐eight ewes were observed from parturition to weaning. Continuous focal animal sampling was used to quantify the duration of maternal behaviors. Ewe feces were collected every 2 days and estradiol concentration was measured with an enzyme immunoassay kit. All lambs were weighed 24 h after parturition and again at 35 days of age. Parity increased sucking, following, grooming, low‐pitched bleat, head‐up and udder‐refusal behavior and decreased aggressive behavior (P < 0.01, P < 0.01, P < 0.05, P < 0.05, P < 0.05, P < 0.05, P < 0.01, respectively), and litter size showed significant effect on sucking, following and low‐pitched bleat behavior (P < 0.05, P < 0.01, P < 0.05, respectively). The lambs of multiparous ewes were significantly heavier than primiparous ewes at birth (P < 0.01) and were significantly heavier at weaning age (P < 0.01). Similar results were founded for birth weight and weaning weight gain in litter size (P < 0.01, P < 0.01, respectively). Estradiol concentration in feces was higher in multiparous ewes than primiparous ewes. Parity and litter size may have effects on maternal behavior during lactation. Ewes that have 2–3 lambs may be more suitable for production of Small Tail Han sheep in China. 相似文献
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神经肽Y基因多态性及其与小尾寒羊产羔数的关系 总被引：1，自引：0，他引：1
本研究旨在阐明神经肽Y(neuropeptide Y,NPY)基因的多态性及其与小尾寒羊产羔数的关系,为绵羊多羔性的标记辅助选择提供科学依据.采用PCR-SSCP技术检测NPY基因全部3个外显子在小尾寒羊、湖羊、特克塞尔和多赛特羊中的单核苷酸多态性,分析该基因对小尾寒羊多羔性的影响.仅引物P1扩增片段存在多态性,在小尾寒羊中检测到5种基因型,在湖羊和多赛特羊中检测到3种基因型,而在特克塞尔羊中仅枪测到1种基因型;测序分析显示,在小尾寒羊中存在CR、TR和TW3种等位基因,在湖羊和多赛特羊中存在CR和TR 2种等位基因,而在特克塞尔中仅存在CR 1种等位基因.TR与CR相比在绵羊NPY基因编码区第93 bp处发生了1个C→T的单碱基突变;TW与CR相比除发生C93T的单碱基突变外,还在130和131 bp发生了GA塞AT的双碱基突变,该突变引起绵羊NPY成熟肽第16位氨基酸由天冬氨酸变为异亮氨酸.对于多态位点C/T,小尾寒羊3种基因型之间产羔数差异均不显著(P＞0.05).对于多态位点GA/AT,RW型小尾寒羊产羔数平均比RR型的多0.56只(P＜0.05).在小尾寒羊5种复合基因型中,TTRW和CTRW型小尾寒羊产羔数差异不显著(P＞0.05);TTRR、CTRR和CCRR型小尾寒羊产羔数差异也不显著(P＞0.05);TTRW和CTRW型小尾寒羊产羔数均显著高于其余3种基因型(P＜0.05).本研究结果初步表明NPY基因GA/AT突变位点的W等位基因是提高绵羊产羔数的1个潜在有效的DNA标记. 相似文献
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本研究采用PCR-SSCP技术对绵羊催乳素受体（PRLR）基因内含子9和外显子10部分核苷酸多态性及其与小尾寒羊、中国美利奴（新疆型）绵羊多胎品系、肉用品系、体大品系、萨福克、无角陶赛特、中国美利奴（新疆型）和德国肉用美利奴产羔数间的关系进行了分析。结果发现：①在绵羊PRLR基因内含子9的第259 bp处,存在C→T转换,BB基因型的小尾寒羊平均产羔数分别较AA和AB基因型提高0.81和0.87只（P<0.05）;②在绵羊PRLR基因外显子10的第304 bp处存在一个G→A转换,该突变导致PRLR基因第387位氨基酸残基由Glu突变为Lys,并使中国美利奴（新疆型）多胎品系中AB基因型的平均产羔数较AA和BB基因型增加0.58和0.80只（P<0.05）;③在绵羊PRLR基因外显子10第571、585和606 bp处分别存在G→A、C→G和C→T突变;其中前2处突变分别导致PRLR基因的第476位氨基酸由Ala突变为Thr,第480位氨基酸由Ser突变为Arg。其中第571 bp处突变导致小尾寒羊AB基因型的平均窝产羔数显著高于AA基因型的窝产羔数,增加0.8 只（P<0.05）。以上结果提示,PRLR基因可能是控制小尾寒羊和中国美利奴（新疆型）绵羊多胎品系产羔数的主效基因或与之存在紧密的遗传连锁。 相似文献
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《中国畜牧杂志》2019,(3)
为探讨TSHR基因多态性与绵羊季节性繁殖和产羔数的关系,本实验采用Sequenom Mass-ARRAY?SNP分型技术检测常年发情的小尾寒羊(407只)、湖羊(101只)、策勒黑羊(48只)和季节性发情的苏尼特羊(21只)、滩羊(22只)、草原型藏羊(161只)TSHR基因的单核苷酸多态性(SNP)位点,并进行群体遗传学分析及其与小尾寒羊产羔数的关联分析。结果表明:TSHR基因g.89430525G>A、g.89363881T>C和g.89431097C>G的基因型频率和等位基因频率在常年发情绵羊品种和季节性发情绵羊品种间差异极显著(P<0.01);这3个SNP位点在大多数绵羊品种中表现为中度多态,且在各个绵羊品种中处于哈代温伯格平衡状态(P>0.05);TSHR基因g.89431097C>G位点的多态性与小尾寒羊第1～3胎的产羔数显著相关(P<0.05),其中CC型母羊的产羔数显著低于CG和GG型母羊(P<0.05)。本研究表明,TSHR基因与绵羊季节性繁殖性状具有较强的相关性,同时参与绵羊季节性繁殖和多羔性状的调控。 相似文献
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Melatonin is thought to be the main molecule that transmits the signal of seasonal change to the neuroendocrine system in seasonal breeding species. Melatonin exerts its effects through specific melatonin receptors, MTNR1A and MTNR1B. In the present study, six native goat breeds in China and one introduced goat breed were analysed to investigate the relationship between the genetic polymorphism of receptor genes and seasonal reproduction. Sequencing results showed that there were five polymorphic mutations in the MTNR1A gene and two in the MTNR1B gene. In the MTNR1A gene, genotypes AA, AB and BB for 424C>T and genotypes CC, CD and DD for 589C>A were observed in these goat breeds. In all six native goat breeds, only genotype AA was detected. In the MTNR1B gene, genotypes EE, EF and FF for 1179G>A and genotypes GG, GH and HH for 1529A>G were detected. However, in Gulin Ma goats, the genotypes EE and HH were not found. Moreover, the base of G at position 1179 and A at position 1529 were linked (By Arlequin ver 3.1, Zoological Institute, Berne, Switzerland, http://cmpg.unibe.ch/software/arlequin3 ,D′ = 0.7496, r2 = 0.4421, χ2 = 489.8679, p = 0.000). Among these mutations, no amino acid change was found in MTNR1A, while both of the mutations in MTNR1B gene caused amino acid changes of R222H and S339G, respectively. The structural analysis showed that the R222H mutation occurred in the first amino acid residue of the third cytoplasmic loop, and the S339G mutation was located in the carboxyl terminus of the protein. In terms of seasonal breeding, all the genotypes we detected showed a similar kidding frequency distribution trend with a higher frequency in May–August than in January–April and in September–December. This suggests that the relationship between the polymorphisms in the MTNR1A and MTNR1B genes and seasonal breeding could not be established. 相似文献
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文章旨在探究绵羊DUSP6基因g.125589716G>A、g.125587728C>T、g.125589714C>T、g.125589006G>A四个位点多态性及其与产羔数之间的关系,以期找到与绵羊高繁殖力相关的分子标记。利用全基因组重测序结合Sequenom MassARRAY誖SNP技术对多羔绵羊品种(小尾寒羊、湖羊、策勒黑羊)和单羔绵羊品种(滩羊、苏尼特羊、萨福克羊、草原型藏羊)DUSP6基因上述4个多态位点进行检测,并与小尾寒羊产羔数进行关联分析。结果表明:绵羊g.125589716G>A位点在多羔绵羊品种中存在AA、GA、GG三种基因型,在单羔绵羊品种中存在GA、GG两种基因型;g.125587728C>T、g.125589714C>T位点在单、多羔绵羊品种中均存在CC、CT、TT三种基因型;g.125589006G>A位点在单、多羔绵羊群体中均只存在GA、GG两种基因型。绵羊DUSP6基因g.125589716G>A位点基因型频率在单、多羔绵羊品种之间差异显著(P<0.05),等位基因频率在单、多羔绵羊品种间差异极显著(P<0.01);g.125587728C>T、g.125589714C>T位点基因型频率和等位基因频率在单、多羔绵羊品种间差异均达极显著水平(P<0.01);g.125589006G>A位点基因型频率和等位基因频率在单、多羔绵羊品种间差异不显著(P>0.05)。关联分析表明,DUSP6基因各SNPs多态性与小尾寒羊不同胎次产羔数之间无显著关联(P>0.05)。综上说明,绵羊DUSP6基因g.125589716G>A、g.125587728C>T、g.125589714C>T以及g.125589006G>A等4个位点的多态性与小尾寒羊各胎次产羔数之间均无显著关联(P>0.05),不适用于小尾寒羊多羔性状选育。 相似文献
13.
《中国畜牧杂志》2019,(6)
为研究KISS1基因在常年发情的小尾寒羊和季节性发情的草地型藏羊中的表达模式,以及KISS1基因多态性与绵羊繁殖之间的关系,实验采用qPCR技术对比分析KISS1基因在2个品种绵羊的10种繁殖相关组织中的表达差异,同时利用Sequenom MassARRAY~?SNP技术对常年发情绵羊(小尾寒羊、策勒黑羊和湖羊)和季节性发情绵羊(滩羊、苏尼特羊和草地型藏羊)KISS1基因2个SNPs位点多态性进行检测,并与小尾寒羊产羔数进行关联分析。qPCR结果显示:KISS1基因在小尾寒羊下丘脑、大脑、垂体和甲状腺中的表达量高于草原型藏羊(P<0.05);分型结果表明,g.1317523C>T位点基因型频率和等位基因频率在常年发情和季节性发情绵羊品种间差异均达到极显著水平(P<0.01);群体遗传学分析表明,g.1317523C>T位点在6个绵羊群体中均表现为中度多态(0.25T位点在小尾寒羊、滩羊、苏尼特羊和湖羊中均处于哈代温伯格平衡状态(P>0.05),g.1311578G>T位点在苏尼特羊和湖羊中处于哈代温伯格平衡状态(P>0.05);关联分析表明,这2个SNPs位点与小尾寒羊前3胎产羔数均无显著关联(P>0.05),但g.1311578G>T位点TT型各胎产羔数均大于GT和GG型。综上,KISS1基因与绵羊的季节性繁殖密切相关,并且g.1311578G>T位点对绵羊产羔性状有潜在调控作用。 相似文献
14.
为揭示FSHβ和LHβ基因在小尾寒羊下丘脑-垂体-卵巢轴(HPOA)中的表达规律,深入了解其对小尾寒羊多羔的作用,本研究采用实时荧光定量PCR技术对6只小尾寒羊(FecB++型单、多羔羊各3只)的生殖组织及脑组织中FSHβ和LHβ基因的表达差异进行分析。结果表明:FSHβ和LHβ基因在大脑、小脑、下丘脑、卵巢、子宫、输卵管和垂体7种组织中均有表达,FSHβ主要在小尾寒羊下丘脑和卵巢高表达,LHβ在垂体高表达;FSHβ基因在小尾寒羊多羔群体下丘脑、卵巢、子宫、输卵管、垂体的表达极显著高于单羔群体(P<0.01),LHβ基因在小尾寒羊多羔群体下丘脑、卵巢、子宫、输卵管、垂体、小脑、大脑表达量均极显著高于单羔群体(P<0.01)。研究结果提示,FSHβ和LHβ基因可能参与小尾寒羊多羔性状调控。 相似文献
15.
《中国畜牧杂志》2019,(4)
本实验旨在探究绵羊MKRN3基因g.275981C>T与g.276999C>T位点多态性与绵羊产羔数之间的关系,以期为绵羊高繁殖力分子育种提供新的遗传标记。利用全基因组重测序结合Sequenom MassARRAY~?SNP技术对常年发情绵羊品种(小尾寒羊、策勒黑羊和湖羊)和季节性发情绵羊品种(滩羊、苏尼特羊和草原型藏羊)MKRN3基因2个多态位点多态性进行检测,并与小尾寒羊产羔数进行关联分析。结果表明:MKRN3基因g.275981C>T位点与g.276999C>T位点均存在3种基因型;g.276999C>T位点基因型频率和等位基因频率在2种发情模式绵羊品种间差异均达到极显著水平(P<0.01);g.275981C>T位点在6个绵羊品种中均表现为低度多态(PIC<0.25),g.276999C>T位点在6个绵羊品种中均表现为中度多态(0.25T位点在苏尼特羊和策勒黑羊中均处于哈代温伯格平衡状态(P>0.05),g.276999C>T位点在6个绵羊品种中均处于哈代温伯格平衡状态;关联分析表明,g.275981C>T和g.276999C>T位点不同基因型与小尾寒羊第1、2、3胎产羔数均无显著关联。可见,MKRN3基因2个多态位点均不适合用于小尾寒羊产羔数选育。 相似文献
16.
FecB基因对杜寒杂交母羊产羔性能及后代生产性能的影响研究 《畜牧与饲料科学》2023,44(1):76-79
[目的] 探究FecB基因对杜寒杂交母羊产羔性能及后代生产性能的影响。[方法] 以杜泊羊（♂）和小尾寒羊（♀）杂交羊横交固定母羊群体为研究对象,检测不同横交固定代次母羊的FecB基因,测定并比较不同FecB基因型母羊的产羔性能及后代生产性能指标。[结果] 在杜寒横交固定一代、二代、三代、四代群体中均检测出BB型、B+型、++型3种基因型,横交固定代数越高,群体中纯合型BB基因型频率越高。杜寒横交固定一代、二代、三代BB型母羊的平均产羔数显著（P<0.05）高于相应横交固定代次的B+型母羊,并且随着群体中BB基因型频率的增加,母羊平均产羔数呈升高趋势。杜寒横交固定一代、二代母羊的平均产羔率低于小尾寒羊母羊,而杜寒横交固定三代母羊的平均产羔率高于小尾寒羊母羊以及杜寒横交固定一代、二代母羊。后代初生生产性能方面,BB型杜寒横交固定三代母羊后代的初生重显著（P<0.05）低于B+型杜寒横交固定三代母羊后代,二者的初生体高、体长、胸围差异不显著（P>0.05）;后代断奶生产性能方面,BB型杜寒横交固定三代母羊后代的断奶重、体长、胸围与B+型杜寒横交固定三代母羊后代差异不显著（P>0.05）,体高显著（P<0.05）低于B+型杜寒横交固定三代母羊后代。[结论] FecB基因对杜寒杂交母羊产羔性能影响显著,在多羔母羊选择中具有很大的应用价值。 相似文献
17.
《中国畜牧杂志》2019,(12)
为探究绵羊Cry1基因g.175355119T>C、g.175357583C>T位点与Cry2基因g.74126865C>T位点多态性与绵羊产羔数之间的关系,本实验利用全基因组重测序结合Sequenom MassARRAY?SNP技术对常年发情绵羊品种(小尾寒羊、湖羊和策勒黑羊)和季节性发情绵羊品种(滩羊、苏尼特羊和草原型藏羊)Cry1基因与Cry2基因共3个多态位点多态性进行检测,并与小尾寒羊产羔数进行关联分析。结果表明:Cry1基因g.175355119T>C位点存在CC、CT和TT 3种基因型,g.175357583C>T位点存在TT、TC和CC 3种基因型;Cry2基因g.74126865C>T位点存在CC、CT和TT 3种基因型。群体遗传学分析表明,Cry1基因g.175355119T>C位点与Cry2基因g.74126865C>T位点的基因型频率和等位基因频率在2种发情模式绵羊品种间的差异均达到极显著水平。在6个绵羊品种中,Cry1基因g.175355119T>C位点与g.175357583C>T位点均表现为中度多态(0.25T位点均表现为低度多态(PIC<0.25)。卡方适合性检验表明,Cry1基因g.175355119T>C位点在6个绵羊品种中均处于哈代温伯格平衡状态(P>0.05),g.175357583C>T位点在苏尼特羊中处于哈代温伯格不平衡状态(P<0.05),Cry2基因g.74126865C>T位点在滩羊和草原型藏羊中处于哈代温伯格不平衡状态(P<0.05)。关联分析表明,3个多态位点与小尾寒羊第1、第2以及第3胎产羔数均无显著关联(P>0.05)。综上,Cry1基因g.175355119T>C、g.175357583C>T位点与Cry2基因g.74126865C>T位点均不适用于小尾寒羊产羔数选育。 相似文献
18.
19.
Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries) 总被引：2，自引：0，他引：2
Chu MX Liu ZH Jiao CL He YQ Fang L Ye SC Chen GH Wang JY 《Journal of animal science》2007,85(3):598-603
The Small Tailed Han is a prolific local sheep breed in China. The bone morphogenetic protein receptor IB (BMPR-IB) gene, which affects the fecundity of Booroola Merino sheep, and the bone morphogenetic protein 15 (BMP-15) gene, which affects the fecundity of Inverdale, Hanna, Belclare, Cambridge, and Lacaune sheep, were studied as candidate genes associated with the prolificacy of Small Tailed Han sheep. Single nucleotide polymorphisms of BMPR-IB and BMP-15 genes were detected in Small Tailed Han ewes (n = 188) by PCR-RFLP. The combined effect of the 2 genes on the prolificacy of Small Tailed Han sheep was studied. The results indicated that the same FecB mutation (Q249R) occurred in the BMPR-IB gene in Small Tailed Han ewes as found in Booroola Merino ewes. The Small Tailed Han ewes with genotypes FecB(B)/FecB(B) and FecB(B)/FecB(+) had 1.40 (P < 0.01) and 1.11 (P < 0.01) more lambs, respectively, than those with genotype FecB(+)/FecB(+). The same FecX(G) mutation (Q239Ter) of the BMP-15 gene was found in Small Tailed Han ewes as in Belclare and Cambridge ewes. The Small Tailed Han ewes with the heterozygous mutant FecX(G)/FecX(+) had 0.55 (P < 0.01) more lambs than those with the wild-type FecX(+)/FecX(+). The Small Tailed Han ewes carrying mutations in both BMPR-IB and BMP-15 genes had greater litter size than those with either mutation alone. In view of our results, marker-assisted selection using both BMPR-IB and BMP-15 genes is warranted to increase litter size in sheep and will be of considerable economic value to sheep producers. 相似文献
20.
M.X. Chu X.C. Wang M. Jin R. Di H.Q. Chen G.Q. Zhu L. Fang Y.H. Ma & K. Li 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2009,126(1):63-68
A single nucleotide polymorphism of 5' flanking region of the prolactin gene was investigated in both high prolificacy breeds (Small Tail Han and Hu sheep) and low prolificacy breeds (Dorset and Suffolk sheep) using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP). The results indicated that two genotypes (AA and AB) were detected in Small Tail Han sheep (n = 239), only one genotype (AA) was detected in Hu (n = 40), Dorset (n = 50) and Suffolk sheep (n = 39). The mutant homozygous genotype (BB) was not detected in four sheep breeds. In Small Tail Han sheep (n = 239), the frequency of genotypes AA and AB was 0.91 and 0.09, the frequency of the A and B alleles was 0.95 and 0.05, respectively. The fitness tests showed that the Small Tail Han sheep population was in Hardy–Weinberg equilibrium. Sequencing revealed a mutation (G→T) at the position 63 bp of the 5' flanking region of prolactin gene in AB genotype compared with AA genotype in Small Tail Han sheep. The Small Tail Han ewes with AB genotype had 0.83 (p < 0.05) lambs more than those with AA genotype. These results preliminarily showed that the prolactin locus is either a major gene that influences the high prolificacy in Small Tail Han sheep or is in close linkage with such a gene. 相似文献