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1.
The aim of this study was to investigate the effects of different cage stocking densities on egg production parameters, as well as related stress and immunity indices in Japanese quails under subtropical Egyptian conditions. Two hundred and sixteen birds of Japanese quail at 14th week of age were used in this experiment. The birds were divided randomly into three groups: 60, 72 and 84. Each group subdivided into 4 replicates, where the cages' floor spaces were 200 (S1), 167 (S2) and 143 (S3) cm2/bird, respectively. Birds housed at 200 cm2/bird (S1) had superior fertility (fertility % (p = 0.013) and hatchability % (p = 0.041)), egg production (egg weight (p = 0.034) and egg mass (p = 0.001)) and immunity parameters (higher geometric mean of antibody titres against Newcastle disease virus, p = 0.024). Furthermore, they had higher internal egg quality score: albumen height (p = 0.003), yolk height (p = 0.023), yolk index (p = 0.006) and Haugh unit (p = 0.035). Birds housed at 143 cm2/bird (S3) had the lowest total leucocytic count and lymphocyte % (p = 0.022), but the highest H/L ratio (p = 0.001). Corticosterone concentration was lower in S1 group (p = 0.031) than that in groups housed at higher densities. Japanese quail housed at high densities revealed drop in fertility, hatchability, production and immunity parameters, indicating a detrimental effects on both welfare and economic income.  相似文献   

2.
An experiment was conducted to evaluate the effects of B. subtilis RX7 and B. subtilis B2A on growth performance, blood profiles, intestinal Salmonella population, noxious gas emission, organ weight and breast meat quality of broilers under S. typhimurium challenge. A total of 120, one‐day‐old Ross 308 male broiler chicks were assigned to four dietary treatments, composed of six replications, with five birds per replication, for 10 day. The dietary treatment groups were negative control (NC; no antibiotic, no B. subtilis), positive control (PC; NC + 0.1% virginiamycin), B. subtilis RX7 (NC + 0.1% B. subtilis RX7 1.0 × 109 cfu/g) and B. subtilis B2A (NC + 0.1% B. subtilis 1.0 × 109 cfu/g). All birds were orally challenged with 2 ml suspension, containing 104 cfu/ml of S. typhimurium KCCM 40253. Results indicated that the body weight gain, feed intake and feed conversion did not differ, among all comparative treatments. Serum haptoglobin concentration was lower in Bacillus treatments (RX7 + B2A) than the NC treatment (p < 0.05). Intestinal and excreta Salmonella number, and excreta ammonia gas emission in the PC treatment or Bacillus treatments, was lower than the NC treatment (p < 0.05). Breast pH, colour and water‐holding capacity were not affected by supplementation of B. subtilis RX7 and B2A. However, drip loss at 1 day post‐slaughter from birds fed with B. subtilis RX7 and B2A decreased, compared with the positive control birds (p < 0.05). Relative gizzard weights of birds fed B. subtilis RX7 and B2A were significantly higher than the NC birds under S. typhimurium challenge. It is concluded from the results that B. subtilis RX7 and B2A increased the gizzard weight and decreased the intestinal and excreta Salmonella population and excreta ammonia gas, and drip loss of breast meat after being stored for 1 day, under stress caused by the S. typhimurium challenge.  相似文献   

3.
The purpose of this study was to evaluate the effect of dietary red yeast (Sporidiobolus pararoseus) on production performance and egg quality of laying hens. A total of 200 Esa Brown laying hens (23 weeks of age) were allocated equally to negative control group (no yeast supplement); positive control group (2 g/kg of Saccharomyces cerevisiae); 0.5, 1, 2 g/kg red yeast respectively. The experiment was lasted for 12 weeks. Feed intake, hen‐day egg production and egg weight were not different between control and supplemented groups. However, yeast‐supplemented groups were significantly improved feed efficiency (p < .05). Incremental levels of red yeast increased the colour score of egg yolk (p < .05). The cholesterol and triglyceride of serum and yolk were significantly (p < .05) lower in the laying hens fed dietary administration red yeast compared to the control diet; however, no significant (p > .05) differences among yeast‐supplemented groups were observed. The hepatic hydroxymethylglutaryl‐coenzymeA (HMG‐CoA) reductase activity was significantly lower (p < .05) in the 2 g/kg red yeast‐supplemented group compared to the control and other red yeast‐supplemented groups. Concentrations of caecal short‐chain fatty acids was increased (p < .05) in laying hens fed 1 and 2 g/kg red yeast as compared to the control group. Dietary administration of 2 g/kg red yeast (S. pararoceus) significantly improved egg yolk colour, decrease serum and egg yolk cholesterol levels.  相似文献   

4.
This study investigated the influence of Bacillus‐based probiotics on performance and intestinal health in broiler challenged with Clostridium perfringens‐induced necrotic enteritis. One‐day‐old Arbor Acre (n = 480) were randomly assigned to four treatments with 10 cages of 12 birds: (a) basal diet negative control (NC), with no probiotics nor antibiotics formulated to contain 2,930 and 3,060 kcal/kg with 24.07 and 15.98% CP, for starter and finisher diet, respectively, (b) basal diet + enramycin (5 mg/kg), an antibiotic growth promoter (AGP); (c) basal diet + Bacillus subtilis B21 at 2 × 109 CFU per g (BS); (d) basal diet + Bacillus licheniformis B26 at 2 × 109 CFU per g (BL); growth performance, intestinal morphology, intestinal lesion scores, short‐chain fatty acids (SCFAs) and mucosal barrier tight junction's (TJ) mRNA expression were assessed. NC‐ and BL‐fed groups showed higher (p = 0.005) average daily feed intake from d1 to d21 than AGP and BS, whereas BS‐ and AGP‐fed groups showed higher average daily weight gain from d22 to d42 and d1 to d42 of age. Higher mortality rate of (12.5%) and lower of (5.5%) were recorded in AGP and NC fed‐groups respectively, lesion score was higher in BS and BL than in AGP, while no lesion was observed in NC group, results revealed higher duodenum and jejunum villus height to crypt depth (VH:CD) compared with NC and BS. Probiotics‐fed groups showed higher total (SCFAs), acetic and butyric acid concentrations at d21 post‐challenge (PC) than other groups. The expression of claudin‐1 was upregulated in duodenum (d7) PC and in jejunum (d7) and (d21) PC in BL group, while at d21 PC, the expression of occludens was higher in jejunum and ileum by AGP and BL. The present study indicated both BS and BL have some similarity with AGP in preventing or partially preventing NE effect in broilers.  相似文献   

5.
ABSTRACT

1. This study was conducted to investigate the effect of multi-strain probiotic (containing Lactobacillus acidophilus 2.5 × 107 cfu/g, Lactobacillus casei 2.5 × 107 cfu/g, Bifidobacterium thermophilum 2.5 × 107 cfu/g and Enterococcus faecium 2.5 × 107 cfu/g) and single-strain probiotic (Pediococcus acidilactici 1 × 1010 cfu/g) on broiler breeder performance and gastrointestinal health.

2. A completely randomised trial was conducted using 300 broiler breeder hens (Ross 308) aged 51 weeks old which were randomly allocated to 1 of 5 dietary treatments with 6 replicates per treatment in a 10 week trial. Treatments included (1) the basal diet a negative control, (2) basal diet supplemented with 0.1 g/kg multi-strain probiotic (MS), (3) basal diet supplemented with 0.1 g/kg single-strain probiotic (SS), (4) basal diet supplemented with 0.1 g/kg of both of probiotics (MS+ SS) and (5) positive control basal diet supplemented with 0.5 g/kg oxytetracycline antibiotic (OX).

3. Body weight, egg production, yolk weight, eggshell thickness and weight, Haugh unit, fertility and hatchability were determined. Results showed that dietary treatments had no significant effect on total hen house or total hatching egg production, egg weight, yolk colour index, shell weight, mortality, body weight, fertility, hatchability, oviduct and stroma weight or number of large and small yellow follicles (P > 0.05). None of the jejunum morphological parameters, apparent ileal digestibility of protein and ileal Lactobacillus population were influenced by supplemental probiotics (P > 0.05), although ileum Escherichia coli count was reduced by inclusion of dietary probiotics (P < 0.05).

4. It was concluded that although both probiotic treatments reduced coliforms, they did not improve broiler breeder performance or gastrointestinal tract (GIT) function.  相似文献   

6.
The aim of this study was to evaluate dietary supplementation of different levels of dehydrated bocaiuva pulp (DBP) on the productive performance of laying quails, and on lipid oxidation, and quality in their eggs. A total of 120 quails were distributed in a completely randomized design with six treatments (0%, 0.1%, 0.2%, 0.3%, 0.4% and 0.5% of DBP), five replicates and four birds per experimental unit. To evaluate lipid oxidation, the eggs were collected on five consecutive days at the end of the trial and distributed in a completely randomized experimental design in a 6 × 5 factorial scheme (eggs from quails fed different levels of DBP × evaluated fresh and after 7, 14, 21 and 28 days). The antioxidant potential of diets was improved (p < 0.05) when the DBP was added estimated as 2,2'‐azinobis‐3‐ethylbenzothiazolin‐6‐sulfonic acid (ABTS) free radical scavenging capacity. No effect (p > 0.05) was observed on feed intake, hen‐day egg production and feed conversion (kg/kg and kg/dz). There were no changes (p > 0.05) in the egg weight, Haugh unit, yolk index or eggshell percentage. Although no difference was observed (p > 0.05), when comparing each level with the control, a negative linear effect (p < 0.05) on specific gravity was observed. In addition, only the treatment with 0.5% pulp exhibited lower (p < 0.05) eggshell thickness compared with the control (0%). Thiobarbituric acid reactive substances (TBARS) analysis showed that as the dietary pulp level was increased, malondialdehyde content decreased linearly (p < 0.05) in the egg yolks, regardless of the storage period. Inclusion of DBP in the diets had no effect (p > 0.05) on the concentration of conjugated dienes in the eggs. The diets containing DBP showed a potential antioxidant effect, reducing the degree of lipid oxidation in egg yolk. However, no beneficial effects were observed on performance and egg quality.  相似文献   

7.
The study was designed to evaluate AndroMed® for the freezability and fertility of Nili‐Ravi buffalo semen. Semen was collected from four adult Nili‐Ravi buffalo (Bubalus bubalis) bulls for 3 weeks (replicate). Semen ejaculates from each buffalo bull were divided into three aliquots. One aliquot was used for evaluation of motility, plasma membrane integrity, livability, viability, DNA integrity and normal apical ridge. Remaining two aliquots were diluted (37°C; 50 × 106 spermatozoa/ml) in tris‐citric egg yolk or AndroMed® extender and cryopreserved in 0.5 ml French straws. After thawing, per cent post‐thaw motility (47.9 ± 0.8, 49.2 ± 1.7), plasma membrane integrity (44.4 ± 1.2, 46.8 ± 1.8) and normal apical ridge (81.4 ± 0.3, 83.2 ± 0.3) were recorded similar (p > .05) in tris‐citric egg yolk and AndroMed® extender. Higher (p < .05) percentage of sperm livability (70.5 ± 1.4 and 64.4 ± 1.0), viability (67.5 ± 1.5 and 61.5 ± 0.6) and DNA integrity (97.0 ± 0.3 and 93.4 ± 0.21) were recorded in AndroMed® compared to tris‐citric egg yolk post‐thaw. Values for all the aforementioned spermatozoal quality parameters were observed lower (p < .05) in frozen‐thawed compared to fresh semen irrespective of the experimental extenders. Fertility rates of buffalo semen did not differ (p > .05) either cryopreserved in tris‐citric egg yolk or AndroMed® extender (45.5% vs. 49%). It is concluded that AndroMed® is capable in protecting the buffalo bull sperm during freeze‐thawing process and can be adopted safely for routine use replacing the tris‐citric egg yolk extender in artificial insemination programme.  相似文献   

8.
The aim was to elucidate the impact of Royal Jelly (RJ) on behavioural patterns, feather cover, egg quality and some blood haematological indices in laying hens (58–64 weeks of age). A total of 108 Tetra Brown laying hens were used in the current trial. The birds were divided into three equal groups (36 birds each). The pure RJ was prepared for immediate injection subcutaneously, as follows: the first treated group (RJ1:100 mg/kg); the second treated group (RJ2:200 mg/kg); the control group. The eating and drinking activities in the RJ2 group were significantly (p = .009 and .015 respectively) higher than the control and RJ1 groups. Furthermore, the aggressive pecks, feather pecks and threating behaviour in the RJ2 group were significantly (p = .005, .001 and .039 respectively) lower than the control and RJ1 groups. Both RJ‐treated groups had the best feather cover on the neck and abdomen regions (p = .010 and .001 respectively; Figure  1 ). Both RJ‐treated groups had a significantly higher eggshell ratio (p = .019) and shell thickness (p = .001) in comparison with the control group. The albumen height, Haugh units and yolk index in both RJ‐treated groups were significantly greater than those recorded in the control group (p = .026, .001 and .022 respectively). The erythrocyte and total leucocyte counts in the RJ2 group were significantly higher than those reported in the control and RJ1 groups (p = .029 and .013 respectively); however, the heterophil/lymphocyte (H/L) ratio and heterophil % in both RJ‐treated groups were significantly the lowest (p = .001 and .039). In conclusion, birds in the RJ2 group had superior feather cover, welfare and behavioural indices, probably due to the impact of active flavonoids components of RJ on laying hen performance. Furthermore, the RJ‐treated groups had significantly improved egg quality parameters and some blood haematological indices.  相似文献   

9.
An experiment was conducted to evaluate the effects of in ovo injection of chrysin, quercetin and ascorbic acid on hatchability, somatic attributes, hepatic antioxidant status and early post‐hatch growth performance of broiler chicks. Four hundred and eighty embryonated broiler breeder eggs containing live 18‐day‐old embryos were divided into six groups of 80 eggs each. One group remained intact and served as a control group (i), whereas the other five groups were injected with the prepared injection solutions as follows: (ii) 0.05 ml distilled water; (iii) 0.05 ml distilled water containing 6 mg ascorbic acid; (iv) 0.05 ml dimethyl sulfoxide (DMSO); (v) 0.05 ml DMSO containing 4.5 mg quercetin; and (vi) 0.05 ml DMSO containing 4.5 mg chrysin. The hatchability rate, hatching weight, residual yolk sac weight, yolk sac‐free body weight, liver weight, hepatic glutathione peroxidase and total superoxide dismutase activities, as well as malondialdehyde concentrations, were not affected by the injected solutions. There were no differences between chicks hatched from the control and in ovo injected eggs in weight gain, feed intake and feed conversion ratio from 0 to 11 days of age. However, the specific contrast performed between the in ovo injected groups and intact eggs revealed that in ovo injection significantly increased hatchability rate (p = .0493). This finding also implies that our injection procedure was harmless. In conclusion, the intra‐egg injection of chrysin, quercetin or ascorbic acid at the injection rates used in this study did not have a significant effect on hatchability, somatic characteristics, early growth performance and hepatic antioxidant status of broiler chicks. However, the overall hatchability was higher in the in ovo injected eggs as compared to non‐injected ones. These findings also confirmed the harmlessness of the procedure developed for in ovo injection in this study.  相似文献   

10.
This research aimed to determine whether the astragalus polysaccharide (AP) can improve the production performance and gut microbiota in Chongren hens.120 Chongren hens (240-d old) were randomly allocated into 4 treatments with 30 hens and fed with a control basal diet (CON) or CON supplemented with the different levels of AP (100, 200, and 400 mg/kg) for 56 d. The egg production and feed conversion ratio were decreased (p < .05) with the levels of AP. The yolk weight, yolk color, eggshell thickness, eggshell redness index and egg shell yellowness were increased (p < .05). AP supplementation increased CAT and T-AOC and SOD, and decreased MDA (p < .05). Supplementation of AP decreased IL-2, IL-6 and TNF-α levels (p < .05), but increased the IL-4 level in the liver (p < .05). The villus heights of duodenum, jejunum ileum, the crypt depth and V/C in the jejunum were increased (p < .05). Dietary supplementation of 200 mg/kg AP increased (P relative abundances of Firmicutes and Lactobacteriaceae in the cecum of Chongren hens. In conclusion, addition of AP improved the production performance, egg quality, antioxidant function, and intestinal morphology in hens, which might be associated with the gut microbiota.  相似文献   

11.
Today, several strategies are being used to decrease the serious effects of antibiotics abuse on broilers industry and public health, among which synbiotics are one of the most promising antibiotic alternative. This study was undertaken to assess the effects of synbiotics, which composed of probiotics (Bacillus subtilis) and prebiotics (xylooligosaccharide and mannanoligosaccharide), on growth performance, intestinal morphology, sIgA content and antioxidant parameters of broilers. Four hundred and fifty one‐day‐old commercial Cobb48 broilers were assigned to five treatments consisting of six replicates of 15 birds each pen. Five dietary treatments include basal diets (control), basal diets plus antibiotics (4 mg/kg Xanthomycin), basal diets plus 1 g of probiotics B. subtilis product/kg of diets (4 × 108 cfu/kg), basal diets plus 150 mg/kg xylooligosaccharide (35%) and 1 g/kg mannanoligosaccharide (75%), and basal diets plus synbiotics (1 g of probiotics B. subtilis product/kg of diets (4 × 108 cfu/kg), 150 mg/kg xylooligosaccharide (35%) and 1 g/kg mannanoligosaccharide (75%). The results demonstrated that on 21 and 42 days, dietary supplementation of the synbiotics significantly increased daily weight gain (p < 0.05), feed efficiency (p < 0.05), the villus height and villus:crypt ratio in the duodenum, jejunum and ileum (p < 0.05), as well as intestinal mucosa sIgA content (p < 0.05), serum T‐SOD activity (p < 0.05) and lysozyme content (p < 0.05), comparing with control group. In conclusion, synbiotics (B. subtilis and xylooligosaccharide and mannanoligosaccharide) is one of the safe and ideal dietary supplementations to increase broilers' growth performance by improving small intestinal morphology, sIgA content and antioxidant capabilities.  相似文献   

12.
The aim of the study was to evaluate the effect of Chinese herbal mixture (CHM) in laying hen diets on performance, egg quality and egg nutrition components, as well as selected blood biochemical parameters. A total of 270 Lohmann Brown laying hens, 24 week of age, were randomly distributed into three dietary treatments and fed for 6 week. The CHM was used at the levels of 0%, 0.5% and 1.0% in the diets of the control and two treatment groups respectively. Each dietary treatment was divided into five replicates, comprising of 18 hens each. Hens were housed in a 3‐tier battery system. Feed and water were provided ad libitum. Blood samples and eggs were collected at the end of the experiment. The results showed that dietary treatments did not affect egg weight, mortality, eggshell thickness, albumen height, Haugh units, eggshell‐breaking strength and egg‐shape index. Dietary supplementation of CHM significantly improved (p < 0.05) laying rate and the lowest feed conversion ratio were observed with 1.0% CHM supplementation diets. CHM supplementation decreased (p < 0.05) cracked‐egg rate, egg yolk cholesterol, blood serum levels of cholesterol, triglyceride, low‐density lipoprotein cholesterol (LDL‐C) and alanine aminotransferase (ALT) and increased blood serum levels of high‐density lipoprotein cholesterol (HDL‐C). No differences in serum total protein, serum albumen, serum glucose and aspartate amino transferase (AST) were observed in hens fed CHM. In conclusion, dietary supplementation with CHM improved egg production and egg quality and reduced serum cholesterol concentrations in laying hens. Dietary supplementation of 1.0% CHM in layer diets may be a feasible means of producing eggs with lower cholesterol and higher egg yolk phospholipid contents for health conscious consumers.  相似文献   

13.
1. A study was conducted to determine the effects of dried broccoli floret (DBF) on egg production parameters, egg quality and apparent nutrient digestibility of laying hens. It was hypothesised that feeding DBF can improve egg quality without adversely affecting layer performance.2. Seventy-two White Leghorn layers aged 62 weeks were randomly allotted to 4 dietary treatments (6 cage replicates with 3 hens each) and fed diets containing 0, 40, 80 and 120 g/kg DBF in feed for 56 d.3. Inclusion of DBF had no effect on feed intake, egg production, feed conversion ratio or egg components. However, egg weight increased (linear effect, P < 0.05) as the level of DBF in the diet increased. Egg breakage strength, eggshell strength and Haugh unit were not influenced by dietary treatments. Egg yolk colour (quadratic effect, P = 0.002) and α-tocopherol concentration (quadratic effect, P < 0.0001) improved as a result of DBF inclusion. Egg yolk cholesterol concentration was not altered by DBF inclusion. Apparent total tract nutrient (dry matter, organic matter and crude protein) digestibility coefficients decreased (linear effect, P < 0.05) as the level of DBF increased.4. Feeding DBF to layers up to 120 g/kg of the diet had no negative effects on feed intake, egg production and feed efficiency and improved egg yolk colour and α-tocopherol concentration. However, total tract nutrient digestibility coefficients were negatively decreased as a result of DBF inclusion.  相似文献   

14.
The current research was conducted to determine the comparison of hatchability and some egg quality characteristics in spotted and unspotted partridge (Alectoris chukar) eggs. The research was carried out on 51 male and 102 female chukar partridges (Alectoris chukar) aged 50 wk, which were raised at the Research and Application Farm of the Faculty of Veterinary Medicine of Selcuk University. In total, 244 spotted and 261 unspotted eggs were used in the research. The hatching eggs were stored at 75% RH and 13°C for 14 d. Thirty spotted and 30 unspotted eggs laid on the same day were used for the evaluation of egg quality characteristics. Egg weight, chick weight, egg weight loss during the hatching period, shape index, hatchability, and some egg quality characteristics were evaluated at the end of the research. Spotted eggs exhibited a higher fertility and hatchability, as well as a lower percentage egg weight loss and embryonic mortality when compared with unspotted eggs. No significant differences were observed between spotted and unspotted eggs for egg weight, chick weight, shape index, specific gravity, albumen height, Haugh unit, yolk color green-red chromaticity (a*), albumen pH, and yolk pH. Spotted eggs had higher eggshell color a*, eggshell color blue-yellow chromaticity (b*), and eggshell strength, as well as a lower eggshell color light-dark chromaticity (L*), yolk color L*, and yolk color b* scores when compared with unspotted eggs. It was demonstrated that spots on the eggshells of chukar partridges (Alectoris chukar) improved their hatchability by affecting various quality characteristics of their eggs. It was concluded that spotted eggs are more suitable for hatching.  相似文献   

15.
The objectives of this study were to investigate the effects of flaxseeds and/or fenugreek seeds as a source of phytoestrogens in laying feed on productive performance, egg quality, yolk fatty acids, plasma profile and antioxidative status in aged laying hens. A total of 120 Bovans Brown hens at 65 weeks of age were housed individually in laying cages in an open‐sided building under a 16‐hr light: 8‐hr dark lighting schedule. Hens were randomly divided into four experimental treatments (n = 30 each): (a) control (basal diet without any supplementation); (b) 1 g/kg of flaxseeds; (c) 1 g/kg of fenugreek seeds; and (d) combination of 1 g/kg flaxseeds and 1 g/kg fenugreek seeds from 65 to 71 weeks of age. Hen‐day egg production and egg mass were significantly increased by dietary supplementation of flaxseeds and/or fenugreek seeds, while feed intake was not affected; thus, feed conversion ratio was improved (p < 0.05). Weights of egg, albumen, yolk and shell were increased by the dietary treatments. Shell thickness and yolk colour were improved by supplementation of flaxseeds and/or fenugreek seeds. Yolk crude protein was not significantly affected by treatments; however, crude fat and calcium contents in yolk were increased and egg yolk cholesterol content was decreased. Interestingly, dietary supplementation of flaxseeds, fenugreek seeds and their combination had a significant positive effect on plasma luteinizing hormone, follicle‐stimulating hormone, oestradiol‐17β and triglycerides. Inclusion of flaxseeds and/or fenugreek seeds in the diets enhanced the serum glutathione peroxidase and superoxide dismutase activities and reduced the malondialdehyde and total cholesterol concentrations in plasma. Dietary supplementation of flaxseeds and/or fenugreek seeds increased α‐linolenic and linoleic acids in egg yolk. It could be concluded that inclusion of mixed sources of phytoestrogens in diets improved laying performance, egg quality, the antioxidative status, hormonal profile and steroidogenesis in aged laying hens.  相似文献   

16.
The aim of the study was to identify beta‐haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty‐one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta‐haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2. The bacteriological cultures, serological tests (Streptex®) and PCR assay allowed identification of Streptococcus canis and Streptococcus dysgalactiae in G1 and G2. Ultramicroscopic studies allowed the observation of M Protein and capsules in strains of S. dysgalactiae and S. canis in G1 and G2. The S. canis strains isolated from G2 showed thicker capsules than S. canis strains isolated from G1 (234 ± 24.2 vs 151.23 ± 28.93 nm; p < .001.). No differences were observed in capsule thickness between strains of S. dysgalactiae isolated from G1 and G2 (210 ± 13.54 vs 211.66 ± 19.67 nm; p > .70). All strains of beta‐haemolytic Streptococcus isolated were penicillin sensitive. Penicillin was administered from EG to 5 days post‐partum in 10 G2 females with isolation of BS (G2A). Saline solution was administered in eleven G2 females with isolation of BS (G2B). Ninety per cent of the puppies survived in G2A and 25% survived in G2B. Our results suggest BS is involved in canine neonatal deaths.  相似文献   

17.
Bacillus species are commonly used as probiotics in the poultry feed industry for preventing infectious diseases and improving productivity by altering gastrointestinal microbiota. The growth parameters of Bacillus subtilis for surfactin production in fermentation and the benefits of surfactin on broiler chickens remain unclear. In this study, we examined the growth parameters of B. subtilis in fermentation and evaluated the effects of surfactin from B. subtilis‐fermented products on Clostridium perfringens‐induced necrotic enteritis and growth performance in broilers. Results showed that the highest viable biomass of B. subtilis was observed at 10% molasses and 2% yeast supplementation during fermentation. The 4‐ and 6‐day fermented B. subtilis products were heat‐, acid‐ and bile‐resistant. Furthermore, the 4‐day fermented B. subtilis products with the highest surfactin concentration showed the maximal antimicrobial activity against pathogens, including Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and C. perfringens. Dietary B. subtilis‐fermented product supplementation in broilers significantly improved intestinal morphology and necrotic lesions under C. perfringens challenge. Bacillus subtilis treatments could enhance broiler productivity, as well as promote bone quality and intestinal morphology. These results together indicate that B. subtilis‐fermented products containing surfactin have potential for the development as feed additives and use as possible substitutes for antibiotics to treat C. perfringens in the poultry industry.  相似文献   

18.
This study aimed to investigate the effect of dietary supplementation with Lactobacillus plantarum and Bacillus subtilis on growth performance, apparent nutrient digestibility and stress‐related indicators in dairy calves. Twenty‐four neonatal Holstein calves were randomly allocated to three treatments: a basal diet with no supplementation (control), the basal diet supplemented with 1.7 × 1010 CFU per head per day (CFU/h.d) of L. plantarum GF103 (LB group) or the basal diet supplemented with a mixture of L. plantarum GF103 (1.7 × 1010 CFU/h.d) and B. subtilis B27 (1.7 × 108 CFU/h.d) (LBS group). Dry matter intake (DMI), average daily gain (ADG), feed conversation ratio (FCR), apparent digestibility of nutrients and stress‐related indicators were measured in this trail. The result indicated that no significant differences were observed in DMI or ADG (p > 0.05), but the FCR was improved in the LB group over the first 12 weeks (p > 0.05). The apparent digestibility of nutrients was not altered by probiotics in week 6 (p > 0.05), but the apparent digestibility of total phosphorus was significantly greater in the LB and LBS groups in week 8 (p > 0.05); additionally, an increase in the apparent digestibility of crude protein was detected in the LBS group (p > 0.05). Oral administration of L. plantarum alone improved the T‐lymphocyte transformation rate on days 58 and 62 (p > 0.05), while adding the mixture of L. plantarum and B. subtilis increased the T‐lymphocyte transformation rate (p > 0.05) but decreased the content of cortisol on day 58 (p > 0.05). No significant differences were detected between the LB and LBS groups in growth performance, apparent digestibility of nutrients and stress‐related indicators (p > 0.05). The results suggested that oral administration of L. plantarum improved growth performance, nutrient digestibility and relieved weaning stress in calves, but no additional effect was obtained by supplementation with B. subtilis.  相似文献   

19.
Effect of extended storage on egg quality, embryo mortality and hatchability in FUNAAB-ɑ chickens was determined. Hatchable eggs (n = 288; weighing 53.2 ± 4.67 g) collected from a flock of FUNAAB-ɑ layer breeder hens aged 32 weeks were stored in egg tray with broad end up under 16 ± 1.5°C for either 0, 4, 8, 12, 16 or 20 d. Before incubation, eight eggs from each group were evaluated for internal and external quality traits. Remaining eggs were set in an incubator and transferred into hatcher on embryonic day 18. Data collected were subjected to one-way analysis of variance. Egg weight loss (EWL; p < .001), surface area (p < .001), yolk diameter (p < .001), inner and outer blastoderm diameters (p < .05) and dead in germ (DIG; p < .001) increased with storage duration while yolk height (p < .001), yolk index (p < .001), albumen weight (p < .05), albumen height (p < .05), albumen index (p < .01), Haugh's unit (HU; p < .05), fertility (p < .001), hatchability of set (HATCHS; p < .001) and fertile eggs (p < .05) decreased. Weight losses of 0, 1.2, 2.2, 3.4, 4.6 and 6.1% were recorded in egg stored for 0, 4, 8, 12, 16 and 20 days respectively. Eggs stored beyond 8 days exhibited higher DIG and lower HATCHS. Shell percentage in 4 days storage (11.4%) was lower (p < .05) than in 16 days storage (13.4%). Shell thickness was similar in eggs stored for 0 to 12 days, but 8 days storage (0.60 mm) had thinner (p < .01) shell than day 16 (0.71 mm) and day 20 (0.73 mm) storage. Internal quality unit (IQU) was higher (p < .05) in fresh eggs (180.4) than in 12 days (167.8) and 20 days (167.8) stored eggs. Extended storage of FUNAAB-ɑ eggs caused EWL, surface area shrinkage, lowered HU and IQU, loss of yolk and albumen quality, increased blastoderm diameters and DIG, and decreased egg fertility and HATCHS from day 8 forward. Storing FUNAAB-ɑ eggs beyond 8 days reduced quality parameters; therefore, other mitigating factors are recommended when storing beyond 8 days.  相似文献   

20.
This study was carried out to evaluate the effects of probiotic Bacillus subtilis C-3102 feed additive on quality characteristics including strength, thickness, and weight of eggshells of Boris Brown laying hens. The control group (n=64) was fed a basal diet comprised of maize and feed rice, whereas the experimental group (n=64) was fed a basal diet supplemented with B. subtilis C-3102 (3×105 CFU/g) starting at 49 weeks of age. From 67 to 69 weeks, all hens were induced to molt using an anorexic program; then, the birds in both groups returned to their respective diets (from 69 to 82 weeks). Eggshell strength, measured six times with 60 eggs selected before the molting treatment, was significantly greater in the C-3102 group than in the control group at 51, 59, 63, and 66 weeks (3.45, 3.44, 3.28, and 3.13 kg/cm2; P<0.05, 0.05, 0.01, and 0.01, respectively). Moreover, eggshell strength—measured three times after the molting treatment—was significantly greater in the C-3102 group than in the control group at 73 and 77 weeks (3.79 and 3.65 kg/cm2; P<0.01 and 0.01, respectively). Eggshell thickness was also significantly greater in the C-3102 group than in the control group at 73 and 77 weeks (0.400 and 0.390 mm; P<0.01 and 0.01, respectively). Fecal samples collected from eight hens of each group at 70 weeks of age after forced molting, showed a significantly higher proportion of Lactobacillus spp. in the C-3102 group (8.94 log CFU/g) (P<0.05) than in the control group (8.63 log CFU/g). Clostridium spp. abundance was significantly lower in the C-3102 group (2.92 log CFU/g) than in the control group (4.3 log CFU/g). These results suggest that C-3102 supplementation improves eggshell quality in aged laying hens, particularly after forced molting.  相似文献   

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