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Our objective was to study the differentially expressed proteins (DEP) in various Malus spectabilis (crabapple) varieties (M. ‘Snowdrift’, M. ‘Hongling’ and M. ‘Hongjin’) in relation to Malus domestica (‘Gala’) and their role in pollination. Our method used a two-dimensional electrophoresis (2-DE) to analyse the differential proteins in the pollen of several crabapples. The 2-DE apples combined with the tandem mass spectrometry (MS-MS) and protein database retrieval helped us to identify the nature and function of DEPs in ‘Gala’ apples and crabapples. We identified 1195 proteins through 2-DE. Among these, six DEPs, namely chloroplast ferritin, Actin, Beta-fructofuranosidase, vacuolarH+-ATPase catalytic subunit, Full = Phosphoglucomutase, and Cytochrome b were identified by MS-MS. This study identified six DEPs among the pollen from the ‘Snowdrift’ crabapple, ‘Hongling’ crabapple, ‘Hongjin’ crabapple, and ‘Gala’ apples. The DEPs included metabolism related proteins, stress/regulatory proteins, and proteins involved in signal transduction. 相似文献
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Plants of six strawberry cultivars were raised under controlled conditions and tested for flowering and yield potential. Short days (SD) at intermediate temperatures for 4 weeks in August induced profuse flowering in subsequent long days (LD) in all cultivars except the late-flowering ‘Malwina’. LD conditions induced flowering only in ‘Nobel’, which has an everbearing parent. ‘Nobel’ and ‘Saga’ exhibited broad temperature adaptation for SD floral induction, which was generally reduced or suppressed at 9 and 27°C. After autumn planting, all cultivars flowered most abundantly in plants raised in SD and intermediate temperatures. Flowering was earliest in ‘Nobel’ and ‘Rumba’. Plants that did not reach floral commitment after 4 weeks in SD continued and completed induction under subsequent natural SD conditions after planting in the field, demonstrating the capability of fractional induction. Berry yield varied in parallel with flowering in the field and was always higher in plants raised under SD conditions. The traditional cultivars ‘Florence’ and ‘Sonata’ out-yielded the more recent cultivars. Some cultivars lost more than two thirds of their initiated flowers during the winter with obvious consequences for their yields. With proper raising management, acceptable yields were obtained after autumn planting even in a cool Nordic climate. 相似文献
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<正>南瓜属葫芦科南瓜属,广泛栽培的有中国南瓜(Cucurbitamoschata)、笋瓜(C.maxima)、西葫芦(C.pope)、黑籽南瓜(C.icifolia)和灰籽南瓜(C.mixta)5个种。我国作为蔬用栽培的是中国南瓜、笋瓜、西葫芦,其中以中国南瓜分布最广,嫩果、老果兼食;其次以食用嫩果的西葫芦,北方早春广为栽培;笋瓜因其含水量高,味淡,主要作饲料或籽瓜栽培,但从 相似文献
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西瓜(Citrulluslanatus)基因目录(下) 总被引：1，自引：0，他引：1
<正>接上期)基因符号特征特性参考文献67.Mdh-l”苹果酸脱氢酶-l”。2个共显性等位基因之一,每基因控制 1条酶带。在普通 28,54西瓜中发现。68.Mdh丫苹果酸脱氢酶.l‘;;2个共显性等位基因之一,每基因 相似文献
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<正>4 小(洋)南瓜的栽培方式和季节安排小(洋)南瓜具有耐寒和低温伸长性较强等特性、可进行冬春早熟栽培、春季早熟栽培、一般露地栽培和秋季栽培。冬春栽培应用大棚覆盖及多层保温设施,早春栽培可用小拱棚半覆盖栽培,秋季栽培后期应覆盖薄膜保温。不同栽培方 相似文献
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We conducted two studies on how highways affect their adjacent habitats by sampling carabid beetles (Coleoptera, Carabidae)
in patches of formerly continuous forest next to highways. (1) We sampled carabids at 14 highway intersections near Helsinki,
Finland. Each intersection (constructed 2–40 years ago) had two forested patches to study: a remnant (0.5–37.4 ha) and, isolated
from the remnant by an intersection lane, an islet (size 0.2–1.8 ha). Pitfall trap catch data (2301 carabids, 25 species)
showed that remnants hosted higher catches of three carabid species, and slightly higher species richness, than islets (patch-size
effect). Time since intersection construction had no apparent effect on carabids. Traffic volume along the intersection lane
determined the assemblage structure of carabids in dry patches, and the abundance of a forest carabid Calathus micropterus. Compared to moist patches, drier patches hosted lower catches of four generalist species; they also had different assemblages
of carabids (habitat-type effect). An interaction between patch size and habitat type for a forest generalist Pterostichus oblongopunctatus indicated that the patch-size effect was dependent on habitat type. (2) We examined possible dispersal of carabids among
forested patches that were separated by highway lanes in Drenthe, the Netherlands. We released 2696 marked individuals of
10 species, and recaptured 376 using dry pitfall traps. We found no evidence for inter-patch movement for nine forest species,
but 22 of 225 recaptured individuals of Poecilus versicolor, an eurytopic open-habitat species, had crossed the highway. Catches of seven forest species were also significantly lower
in the road verges, compared to the adjacent forests. These two studies suggest that (i) decreasing patch size negatively
affects forest-carabid catch and overall species richness, (ii) habitat type can affect the intensity of the patch-size effect,
(iii) carabid assemblages of forest fragments vary with traffic volume (which may be linked with urbanization), (iv) forest
carabids rarely cross highways, and (v) open habitats associated with road margins are dispersal barriers for forest carabids. 相似文献
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对杨属黑杨组(Aigeiros) 的15个种(品种) 核型进行了分析, 结果如下: 箭杆杨( Populusnigra var. thevestina) , 2n = 38 = 3M + 29m + 5 sm + 1 st; 盖杨( P. del.cv. ×Lux ×P.×canadensis cv. Shanhaiguanensis) , 2n = 38 = 2M + 27m + 4 sm + 5 st (4SAT) ; 欧美杨107 (P1 ×euram ericana cv. ‘74 /76’) , 2n = 38 = 1M + 29m (1SAT ) + 5 sm + 3 st (3SAT) ; 欧美杨13 ( P. ×euramericana CL 13) , 2n = 38 = 3M + 23m + 6 sm + 3 st (1SAT ) + 3 t (3SAT) ; 加杨( P. ×canadensis Moench) , 2n = 38 = 1M + 27m + 6 sm + 4 st ( 4SAT) ; 辽杨×美洲黑杨(P.maximowiczii Henry ×P.deltoids Bartr. ) , 2n = 38 = 1M + 24m + 9 sm (2SAT ) + 4 st; 中尚8号( P. ×Zhongshangnensis) , 2n = 38 = 4M + 27m (1SAT ) + 2 sm + 3 st (2SAT ) + 2 t; 北京杨( P. ×beijingensis) , 2n = 38 = 3M + 28m + 3 sm + 4 st (2SAT) ; 鲁山杨( P. ×Liaoningensis) , 2n = 38 = 1M + 27m + 3 sm + 6 st (1SAT ) + 1 t (1SAT) ; 廊坊1号( P. CL ‘Langfang 1’) , 2n = 38 = 3M + 28m + 3 sm + 4 st (2SAT) ; 圣山杨(盖杨类) (P. ×gaixianesis) , 2n = 38 = 2M + 27m + 4 sm (1SAT ) + 4 st (3SAT ) + 1 t; 抗- 2 〔P. deltoids CL 2 (GMO) 〕, 2n = 38 = 26m + 7 sm (2SAT ) + 4 st (1SAT ) + 1 t (1SAT) ; 群改3号( P. ×Qungainensis 3) , 2n = 38 = 1M + 27m + 8 st (2SAT ) + 2 t (2SAT) ; 中金2号( P. ×Zhongjinnensis 2) , 2n = 38 = 1M + 18m + 13 sm (1SAT ) + 5 st (2SAT) + 1 t (1SAT) ; 辽宁杨( P. ×eur. cv. San Martino ×P. ×canadensis cv. Shanhaiguanensis) , 2n = 38 = 2M + 26m + 6 sm + 4 st。黑杨各种(品种) 核型均为2B型, 种(品种) 间核型差异主要表现在中部着丝点(M、m) 染色体数目(25～32) 和端部着丝点( st、t) 染色体数目变化(1～6) 上, 核型特征为种(品种) 鉴定提供了一定参考。 相似文献
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西瓜(Citrullus lanatus)基因目录(上) 总被引：1，自引：0，他引：1
１９９９ 年在国际葫芦科遗传协会年报（ＣＧＣ Ｒｅｐｏｒｔ）第 ２２期发表了最新的 １份西瓜基因目录，它包括 １５５个基因，特全文译出，供读者参考。 相似文献
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十、糖醋柴把菜(土从) 此烹调方法与上面椒盐柴把鸡(土从)的烹调方法相似,不同的是去掉椒盐和龙虾片、咸酱油,而改用白糖1.5公两,麸醋1.5公两,蚕豆水粉1公钱加水1公两,用炒锅置中火上熬煮搅均匀后制得糖醋汁水浇入盘中,即得糖醋柴把鸡(土从)。 相似文献
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ZHANG Na-na LI Mao-lian BIAN Yun-fei GAO Fen YANG Zhi-ming YANG Hui-yu XIAO Chuan-shi 《园艺学报》2011,27(8):1485-1489
AIM: To observe the effects of angiotensinⅡ (AngⅡ) and angiotensin-(1-7) on the expression of (pro)renin receptor in rat vascular smooth muscle cells.METHODS: The cultured VMSCs were randomly divided into control group, AngⅡ group, Ang-(1-7) group, AngⅡ+losartan (an AT1 receptor antagonist) group, AngⅡ+PD123319(an AT2 receptor antagonist) group and CGP42112A (an AT2 receptor agonist)group. The expression of (P)RR at protein and mRNA levels was detected by Western blotting and real-time PCR,respectively.RESULTS: Compared with control group, AngⅡ distinctly increased and Ang-(1-7) decreased the expression of (P)RR mRNA and protein in VMSCs in a dose-dependent manner (P<0.01). Compared with AngⅡ group, losartan did not inhibit the expression of (P)RR mRNA and protein in VMSCs induced by AngⅡ (P>0.05), but PD123319 did (P<0.01). CGP42112A also induced the expression of (P)RR protein and mRNA in VMSCs (P<0.01).CONCLUSION: AngⅡ induces the expression of (P)RR in VMSCs by AT2. However, Ang-(1-7) inhibits the expression of (P)RR in VMSCs. 相似文献
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