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1.
This research is to examine the reducing power of bacterial single strain and the mixture of strains. Four strains of Dietzia sp. KDB1 (KC433534), Nesterenkonia sp. KDB2 (KC433535), Nesterenkonia sp. KDB3 (KC433536), and Nesterenkonia sp. KDB4 (KC433537) separated from the fermented indigo vat were cultivated in aerobic condition. Natural indigo of the niram powder obtained from the water extract of Polygonum tinctorium and synthetic indigo purchased was used after sterilization. Natural and synthetic indigo samples were reduced with the strains in an incubator. Oxidation/reduction potential and pH of the mixture of indigo and strain(s) solutions were measured at the same intervals of elapsed time. Dyeing test was applied to evaluate the reduction power of bacterial strains isolated. Ramie fabric was used for the indigo dyeing and its color strength was determined from the reflectance at 660 nm and expressed as K/S values by using a spectroscope. CIELAB coordinates were measured with the same spectroscope and H V/C values were obtained by using the conversion program. All the strains showed reducing power onto natural and synthetic indigo samples. With the elapsed time, dye-uptake was increased up to maximum dye-uptake, and then decreased. The potential was dropped rapidly around ?500 mV and then changed very slightly maintaining lower than ?400 mV. The pH measured was decreased with the elapsed time. All the hue values obtained were of bluish purple (PB) shade. On the reducing power represented as dye-uptake, KDB1 strain was the highest among the strains selected for natural indigo and KDB3 strain for synthetic indigo. KDB2 showed the lowest for both of natural and synthetic indigo. Mixture of strains shortened the start time of reduction and that of maximum dye-uptake than any single strain.  相似文献   

2.
Using banana peel extract as an antioxidant in freshly squeezed orange juices and juices from concentrate was evaluated. Free radical scavenging capacity increased by adding banana peel extracts to both types of orange juice. In addition, remarkable increases in antioxidant capacity using 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical were observed when equal or greater than 5 mg of banana peel extract per ml of freshly squeezed juice was added. No clear effects were observed in the capacity to inhibit lipid peroxidation. Adding 5 mg banana peel extract per ml of orange juice did not substantially modify the physicochemical and sensory characteristics of either type of juice. However, undesirable changes in the sensory characteristics (in-mouth sensations and colour) were detected when equal or greater than 10 mg banana peel extract per ml of orange juice was added. These results confirm that banana peel is a promising natural additive that increases the capacity to scavenge free radicals of orange juice with acceptable sensory and physicochemical characteristics for the consumer.  相似文献   

3.
This study compares the antioxidant activity of ten different pomegranate cultivars grown in Iran using the ferric reducing power assay (FRAP assay), which is based on the reduction of a ferric-tripyridyl triazine complex to its ferrous, colored form in the presence of antioxidants. Aqueous solutions of known Fe(+2) concentration, in the range of 100-1000 micromol L(-1) were used for calibration. The results showed that among pulp and peel fractions the sour alac and sweet white peel cultivars had more FRAP value respectively. The pomegranate peel extract had markedly higher antioxidant capacity than the pulp extract. The peel extract of sweet white peel cultivar appeared to have more potential as a health supplement rich in natural antioxidants compared to the pulp and peel extracts of other pomegranate cultivars.  相似文献   

4.
This paper explores the potential uses of the aqueous extract of Vitis vinifera L. leaves in dyeing linen and silk fabrics without a metal mordant. The focus will be on investigating the tinctorial potential of Vitis vinifera L. leaves and its dyeing properties. The anthocyanins amounts of leaves from different Vitis vinifera L. varieties were determined by the spectrophotometry method. The effect of the main dyeing parameters (pH of the dye bath, temperature, and dyeing duration) on the dyeing quality results has been studied. Tannic acid and pomegranate peel extract have been used as a natural mordant. Experimental results have shown that fastness properties of dyed fabrics ranged from average to very good.  相似文献   

5.
本文以闽侯野生蕉(阿宽蕉,Musa itinerans)果皮为研究对象,对不同颜色(绿色和紫色)果皮进行转录组测序,旨在探讨miRNA在野生蕉不同颜色果皮转录后水平中发挥的作用及其可能参与的调控通路,为芭蕉属植物果皮颜色分子调控机制的研究奠定基础。取野生蕉同一果梳上不同颜色(紫色和绿色)的果皮为材料,利用高通量测序和生物信息学分析,获得野生蕉不同颜色果皮组织的miRNA表达谱,筛选不同颜色果皮差异表达的miRNA并预测相关靶基因,通过靶基因分析,获得与调控野生蕉果皮颜色可能相关的信号通路。结果从不同颜色野生蕉果皮中共获得34.11兆条原始读长,经过滤后得到28.73兆条clean reads,从中鉴定出132个已知的miRNA序列和122个新的miRNA;筛选出36个差异表达miRNA,其中10个在紫色果皮中上调表达,26个在绿色果皮中上调表达。表达量最高的miRNA为mit-miR167,而差异倍数最大的为mit-miR172a-3p-2。差异表达miRNA共预测出1164个靶基因可能参与野生蕉果皮颜色调控,并主要在DNA结合、离子跨膜运输活动等功能和植物病原互作等途径富集。其中,有7个基因直接与苯丙烷生物合成途径相关,主要受到miR156和miR482家族的调控。同时,选取6个差异表达miRNA进行定量验证,结果表明,3个miRNA在绿色果皮中上调表达,3个miRNA在紫色果皮中上调表达,定量结果与高通量测序的结果一致。本文通过高通量测序获得了野生蕉果皮miRNA表达谱,并通过生物信息学分析得到可能和调控野生蕉果皮颜色性状相关的miRNA和代谢通路。综上所述,野生蕉果皮颜色可能受到多种miRNA的调控,并涉及多个代谢通路,这有助于进一步了解果皮花青素转录后水平的调控过程,并对进一步的功能验证奠定基础。  相似文献   

6.
研究老挝琅勃拉邦与广西在同一施肥条件香蕉在果指特征、品质指标以及果皮、果肉中氮、磷、钾元素含量的差异,结果发现:外观性状与内在品质无显著性差异,老挝香蕉瓤皮比高于广西香蕉,且果皮色泽比广西香蕉好;老挝香蕉与广西香蕉果皮中氮、磷、钾元素含量存在显著性差异,果肉中只有氮含量存在显著性差异,果肉中磷、钾含量吾显著性差异。本研究结果为香蕉的施肥调控管理以及市场定位提供了一定的参考价值。  相似文献   

7.
Development of an innovative biotechnological method for potato peeling, closer to the ‘ideal’ peeling conditions of the product, was the main objective of the present research. Studies on enzymatic hydrolysis of potato peel were conducted. The efficacy of enzymatic peel hydrolysis was expressed in terms of reducing sugar content of the enzyme solution in which potato peel was incubated. Enzyme screening revealed that an enzyme solution containing a cellulase-xylanase mixture and amylase in a ratio of 1:1 showed good peel hydrolyzing efficiency of peeled peels. To further maximise the peel hydrolysis, condition parameters were optimised using response surface methodology (RSM). At the optimised conditions of 60 °C, pH 6 and 4 h, the reducing sugar yield in the solution was maximum. Characterization of the potato peel using microscopic techniques (scanning electron microscopy (SEM), atomic force microscopy (AFM)) further illustrated degradation of cell wall and reduction in the surface roughness of the peel after enzymatic treatment, which could enhance peel loosening from the intact potato. To further ascertain the efficiency of the process, studies were conducted with the selected enzyme on intact potato tubers under optimised conditions. Easy removal of peel was observed in enzyme-treated potato tubers, which showed 0.52% peel loss by abrasive peeling. The present process employing enzymes could be applied for peeling of intact potato as an alternative to conventional peeling process.  相似文献   

8.
香蕉果实采收后果肉中腐胺(PUT)含量随贮放时间的延长而逐渐下降,在果实出现乙烯高峰后又明显增加。果实采后初期亚精胺(SPD)、精胺(SPM)含量逐渐增加,果实出现乙烯高峰后,精胺明显积累,但亚精胺的变化较小。果皮中精胺的变化与果肉中的相似,但腐胺、亚精胺的变化则不同。精氨酸脱羧酶(ADC)和鸟氨酸脱羧酶(ODC)在果实采后初期活性较高,果实明显衰老时活性迅速降低。果肉中S—腺苷甲硫氨酸脱羧酶(SAMDC)活性先有增加,然后下降;而果皮中SAMDC则呈下降趋势。1——氨基环丙烷—1—羧酸合成酶(ACCS)变化与SAMDC不同,酶活性最高值较乙烯高峰提前二天到达。S—腺苷甲硫氨酸(SAM)在乙烯高峰出现时含量明显下降。结果认为,多胺对果实后熟、衰老的调节机理不但与竞争SAM有关,还与代谢平衡有关。  相似文献   

9.
以3个香蕉品种‘巴西蕉’‘粉蕉’‘皇帝蕉’为供试材料,利用福林酚法测定其整个生育及成熟期果皮、果肉的多酚含量,探索不同香蕉品种在发育成熟过程中的多酚含量变化规律,及其与果实发育成熟的关系。结果显示,在香蕉果实整个生育期,多酚物质含量果皮均大于果肉。多酚物质在香蕉果皮、果肉生长发育期迅速积累,到采收期下降,采后果皮多酚物质含量随着成熟度的增加而增加,而果肉多酚物质基本维持不变。用外源乙烯和1-MCP处理后发现,与对照果实相比,乙烯促进其多酚物质的积累,而1-MCP抑制其积累。  相似文献   

10.
采用室内与田间试验相结合的方法研究了枯草芽孢杆菌T122F菌剂对香蕉生物效应的影响。结果表明:菌剂能明显促进香蕉生长,主要表现为香蕉株高、假茎周长、叶片数以及叶绿素含量的增加。菌剂的施用还能显著提高香蕉内生细菌数量、叶片耐菌能力和根际土壤细菌数量。在300倍液处理下,从香蕉苗根部分离到的菌量为299.75×103 cfu/g FW,是对照的7.16倍,而从香蕉根际土壤中分离到的菌量则是对照的2.20倍。此外,经菌剂处理的香蕉根际土壤水浸液对香蕉枯萎病菌孢子萌发也表现出明显的抑制作用。  相似文献   

11.
Water extract of banana (Musa sapientum) infructescence stalks has been used in folk medicine in the treatment of diabetes mellitus. This work aims at verifying the claimed effect and elucidating its possible mode of action. The extract was given in replacement of drinking water to diabetic rats, and its mechanism of action was studied by investigating its involvement in glucose transport in Caco-2 monolayers, and in rat jejuna using an in situ perfusion technique. Its effect on the Na+/K+ ATPase was studied by measuring the amount of inorganic phosphate liberated. The extract reduced significantly blood glucose levels in diabetic rats and glucose transport across rat jejuna and Caco-2 monolayers, and induced a 50 % decrease in their Na+/K+ ATPase activity. The extract did not induce any further decrease in jejunal glucose uptake in the simultaneous presence of phloridzin and phloretin, respective inhibitors of SGLT1 and GLUT2 transporters nor did it induce a change in the protein expression of SGLT1 and GLUT2. It was concluded that the extract acts by reducing the Na+/K+ ATPase activity of enterocytes and consequently the sodium gradient required for sugar transport by SGLT1, which leads to down-regulation of GLUT2 and contributes to the observed anti-hyperglycemic effect.  相似文献   

12.
Quantitative adsorption kinetic and equilibrium parameters for indigo carmine dyeing of silk were studied using UV-visible absorption spectroscopy. The effect of initial dye concentration, contact time, pH, material to liquor ratio (MLR), and temperature were determined to find the optimal conditions for adsorption. The mechanism of adsorption of indigo carmine dyeing onto silk was investigated using the pseudo first-order and pseudo second-order kinetic models. The adsorption kinetics was found to follow a pseudo-second-order kinetic model with an activation energy (E a) of 51.06 kJ/mol. The equilibrium adsorption data of indigo carmine dye on silk were analyzed by the Langmuir and Freundlich models. The results indicate that the Langmuir model provides the best correlation. Adsorption isotherms were also used to obtain thermodynamic parameters such as free energy (ΔG o), enthalpy (ΔH o), and entropy (ΔS o) of adsorption. The negative values of ΔG o and ΔH o indicate the overall adsorption process is a spontaneous and exothermic one.  相似文献   

13.
本研究采用田间试验方法,对化肥减量模式下山地香蕉的经济产量、经济系数、碳排放量、碳固定量进行测量与估算。结果表明:与常规施肥相比,化肥减量20%时香蕉经济产量无显著性变化,化肥减量达到30%时香蕉产量显著降低24.1%。化肥分别减量10%、20%、30%后,香蕉植株碳固定依次减小2.15%、3.51%、11.33%;化肥产生碳排放依次减小10.01%、10%、10%;土壤碳排放除化肥减量10%处理增加12.67%,其余依次减小16.94%、3.41%。综合香蕉碳固定与碳排放二者的碳收支,并用每株香蕉的碳净排放量来估量,常规施肥、减量10%、减量20%、减量30%的处理每株香蕉的碳净排放量分别为4731.29、5529.03、4418.47、4415.30 g/株,在保证产量前提下化肥减量20%固炭减排潜力最大。本研究结果显示合理的化肥减量能获得山地香蕉种植经济效益与生态效益的共赢,且试验条件下化肥减量20%效果最佳。  相似文献   

14.
研究茶树油(TTO)处理对香蕉果实采后病害控制和贮藏期果实品质的影响。结果表明:利用1.25 g/L TTO熏蒸处理能够有效控制香蕉果实的发病,推迟了果实果皮的褪绿变黄,延缓了果肉硬度和淀粉含量的下降,维持了较高的可溶性糖含量,有利于保持果实的外观和品质;同时随着果皮表面残留TTO的迅速挥发,果实保持了自身较好的风味。TTO作为一种天然的防腐剂,在果蔬采后防腐保鲜方面具有应用前景。  相似文献   

15.
在特定温度条件下,用胡椒、假药、糯米香等植物叶片处理香蕉果实后熟,试验结果表明参试植物叶片处理均有催化香蕉后熟作用,当温度25~30℃,叶片与香蕉质量比约1:5时,后熟香蕉果皮颜色较好,其中,假药叶处理香蕉后熟时间短,果皮颜色好;糯米香叶能延长香蕉果皮褪绿时间。  相似文献   

16.
Kim  Sam Soo  Leem  Su Gyung  Ghim  Han Do  Kim  Joon Ho  Lyoo  Won Seok 《Fibers and Polymers》2003,4(4):204-209
The effect of padding solution on the microwave heat dyeing of polyester fabric was studied extensively. Polyester fabrics were impregnated in aqueous urea solution and aqueous sodium chloride solution for 10 min and then dyed for 7 min by microwave apparatus (2 450 MHz, 700 W) under optimum conditions which provide good exhaustion. Aqueous solutions of urea and sodium chloride showed more effective than water as a padding solution for microwave heat dyeing. The type of solvent added in padding media and its concentration significantly affects the K/S values of dyed polyester fabric. Added solvents such as n-hexane, acetone, and dimethyl formamide were also more effective than 100% water as padding media for the microwave heat dyeing. It is supposed that the effect of used solvents on dyeing property of polyester fabrics depends on the solubility parameter difference between solvent and polyester fabric.  相似文献   

17.
以芦丁与Vc为对照,测定云南普洱茶不同溶剂提取物清除DPPH自由基、羟自由基、超氧阴离子自由基、亚硝酸盐的能力及其还原力.结果表明:普洱茶不同溶剂提取物的抗氧化活性与其浓度成正相关,对DPPH自由基、羟自由基与超氧阴离子有较强的清除作用,对亚硝酸盐的清除作用不明显,具有一定的还原力.其中,乙醇沉析物、正丁醇提取物、乙醇提取物、乙醇溶解物对DPPH.自由基清除效果较好,相同浓度下优于芦丁;水提取物、乙醇沉析物及乙酸乙酯提取物对羟自由基清除效果较好,但效果不如Vc;乙酸乙酯提取物对超氧阴离子自由基和亚硝酸盐的清除作用较好,还原力也很强.  相似文献   

18.
Among extracts of 33 plant species screened against Mycosphaerella eumusae, the causal agent of Eumusae leaf spot disease of banana, water extract of Cassia senna, Zimmu (Interspecific hybrid between Allium cepa × Allium sativum) and Rhincanthus nasutus provided 100% inhibition of spore germination and 1.7–2.0 cm zone of inhibition of mycelial growth under in vitro conditions. The maximum efficacy of mycelial inhibition was observed with Zimmu leaf extract. When Zimmu leaf extract was tested at different concentrations (5, 10, 25, 50 and 100% w/v), all tested concentrations provided complete inhibition of mycelial growth of the pathogen. The field evaluation of Zimmu leaf extract at different concentrations in cv. Grand Naine showed that the application of the water extract of Zimmu leaf at 50% concentration (w/v) provided 55% reduction of disease severity compared to the unsprayed control. Besides, the application of Zimmu leaf extract increased the value of youngest leaf spotted-0 (up to 60.5%) as well as increased the yield of banana (up to 46.8%) as compared to control. The effect of Zimmu in increasing the value of YLS-0 and the bunch yield was comparable with the chemical fungicide Propiconazole 25% EC (0.1%). Thin layer chromatography (TLC) analysis showed that among different major compounds, two lipid compounds (LP-B1 and LP-B2) extracted using methanol had the ability to inhibit M. eumusae growth (0.7–1.5 cm zone of inhibition). The gas chromatography–mass spectrometry (GC–MS) analysis of lipid bands revealed the presence of six different lipid compounds, which may be responsible for the growth inhibition of the pathogen. Since the application of water extract of Zimmu was found to be not only effective in controlling the leaf spot disease severity but also increased the number of green leaves and yield of banana fruits, the Zimmu extract can be used effectively in integrated disease management of Eumusae leaf spot disease for enhancing banana production in an ecologically sustainable manner.  相似文献   

19.
In this paper, the effect of water hardness, expressed in CaCO3 equivalent, on the dyeing silk with acid dye under acid, alkaline and isoelectric point dyeing conditions was studied by zeta potential method. Under acid condition and in the presence of calcium ion, the positive zeta potential of silk was found to decrease with a reduction in the dye adsorption. Such a phenomenon might be due to the presence of cation which increased the dyeing potential barrier at the interface between fibre and dye solution. This would result in a higher resistance of dye anions passing through the interface. Under alkaline condition, the zeta potential on silk was negative and resulted in a strong potential barrier for the dye anions. The presence of calcium ion would result in a decrease in the absolute value of zeta potential of silk fibre with an overall increase in dye absorption. Under isoelectric point, the zeta potential of silk fibre was found to be near zero and dye adsorption was not influenced by the cations. When studying the effect of calcium ion on the silk dyeing with acid dye under acid, alkaline and isoelectric point dyeing conditions, it was found that calcium ions influenced dye exhaustion and fixation greatly under acidic and alkaline dyeing conditions, which such influence became much smaller when the pH of dyebath was at the isoelectric point of the fibre. These results showed that calcium ion could produce strong electrolytic effect on dyeing even under very low concentrations.  相似文献   

20.
This study was carried out to optimize dyeing conditions of unripe Citrus Unshiu extract on silk fabric and to evaluate antimicrobial activity of the dyed fabric for its potential use as a functional natural dye. Unripe fruits of Citrus Unshiu in Jeju Island, Korea, extracted in 80 % Ethanol solution to final solid dye powder were dyed on silk fabric under a variety of conditions such as dye bath concentrations, temperature, and dyeing duration together with mordanting. Dyeing fastness properties to washing, rubbing, perspiration, and light were tested and the antimicrobial activities of the dyed fabric against Staphylococcus aureus and Klebsiella pneumoniae were investigated quantitatively. As results, the fabric showed the maximum dye uptake (K/S) under the conditions of 80 °C for temperature, 30 min for duration, and 600 % (o.w.f.) for dye concentration. Both pre- and post-mordanting seemed not to be effective on increasing K/S values of the dyed silk fabrics under the optimum dyeing conditions. Fastness ratings to washing, rubbing, and perspiration were all very good (4–5 grades) for both 300 and 600 % dyed silk fabrics. Excellent antimicrobial activities over 99 % reduction rate against two both bacteria were exhibited for all of dyed fabrics undergone more than 300 % of dye concentration. From these results, it was concluded that the dye concentration of 300 % of unripe Citrus Unshiu could be employed to produce antimicrobial silk fabric. Furthermore, to get more saturated shades on the fabric by the citrus, higher dye concentration such as 600 % was available as well.  相似文献   

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