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桦褐孔菌多糖对感染弓形虫小鼠治疗作用及其对血清中细胞因子含量的影响 总被引：3，自引：1，他引：2
建立小鼠弓形虫病模型后,将小鼠随机分成6组,统计小鼠存活率及时间,再应用ELISA检测血清中IFN-γ、IL-2等细胞因子水平,以检测桦褐孔菌多糖对感染弓形虫小鼠的治疗效果及对小鼠血清中IFN-γ、IL-2细胞因子水平的影响。结果表明,桦褐孔菌多糖中剂量组小鼠存活率最高;桦褐孔菌多糖的高、中剂量组IFN-γ水平极显著高于其他各组(P<0.01),高剂量组IL-2水平与其他各组比较差异极显著(P<0.01);除感染组外,其余各组IFN-γ、IL-2均在第6天出现峰值。说明桦褐孔菌多糖中剂量治疗弓形虫感染小鼠效果最好,并且中剂量可使小鼠血清中IFN-γ、IL-2值升高到适当水平,有效改善弓形虫感染对小鼠的伤害。 相似文献
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桦褐孔菌多糖对弓形虫感染小鼠基因表达谱的影响 总被引：1，自引：0，他引：1
探讨桦褐孔菌多糖对感染弓形虫小鼠基因表达谱的影响,并对其抗虫机理进行分析。BABL/C小鼠腹腔接种RH株弓形虫速殖子103个,建立小鼠弓形虫感染模型。将小鼠随机分成3组:空白对照组、感染对照组、桦褐孔菌多糖治疗组。采集动物肝脏迅速冻存,提取总RNA,检测RNA质量,扩增RNA,进行芯片杂交,扫描后进行数据分析。结果:经SOM分析筛选出桦褐孔菌多糖治疗组的有效差异基因,并对其进行功能注释,分析其治疗弓形虫的主要原因。结果表明:桦褐孔菌多糖可有效治疗弓形虫感染引起的病理损伤,其抗弓形虫机理是通过调节宿主细胞因子水平,促进Th1/Th2平衡,控制Toll样受体信号通路以及对机体整体调控达到抗弓形虫目的。 相似文献
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《畜牧与兽医》2015,(1):94-97
为了探讨桦褐孔菌多糖对感染不同毒株弓形虫小鼠的早期作用机制,将健康鼠随机分成3组,建立感染不同毒株弓形虫小鼠模型后,应用ELISA方法检测血清中IL-6、TNF-α细胞因子水平。结果:感染RH株与感染PLK株的弓形虫小鼠血清中IL-6、TNF-α的结果相比较,2种毒株的模型组于感染后的值均呈现上升趋势,分别在48和24 h达到峰值,而后呈现下降趋势,但感染RH株的模型组表达量在12~60 h比感染PLK株更高;2种毒株的试验组(桦褐孔菌多糖治疗)于12~60 h期间IL-6、TNF-α的值均一直高于阴性组,低于模型组。说明感染RH株弓形虫小白鼠与感染PLK株弓形虫小白鼠相比较更能刺激其血清中IL-6、TNF-α的过度表达,桦褐孔菌多糖能调节感染不同毒株弓形虫小鼠IL-6、TNF-α的表达。研究表明,桦褐孔菌多糖通过提高机体的免疫功能达到抗弓形虫的目的。 相似文献
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为了探讨桦褐孔菌多糖对急性感染弓形虫小鼠核转录因子c-Jun、IL-1β和IL-12的影响,本试验在建立急性感染弓形虫小鼠模型后,随机将其分成3组:阴性组、急性感染弓形虫小鼠模型组和桦褐孔菌多糖试验组。采用RT-PCR法检测肝组织中c-Jun及脾组织中IL-1β和IL-12的mRNA表达;Western blot法检测c-Jun蛋白的表达。结果显示:c-Jun、IL-1β和IL-12的mRNA分别在360、736和978 bp处扩增出单一电泳条带,且c-Jun蛋白在39 ku处表达,均呈现出弓形虫感染组表达量最高,桦褐孔菌多糖试验组次之,对照组最弱。说明桦褐孔菌多糖可能通过下调c-Jun过度表达,抑制IL-1β、IL-12的过度表达,部分阻抑了AP-1信号通路转导,从而减轻炎症反应,达到抗弓形虫感染的目的。 相似文献
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《黑龙江畜牧兽医》2016,(24)
为了研究桦褐孔菌多糖对链脲佐菌素(STZ)诱导后的C57BL/6糖尿病模型小鼠的糖脂和免疫功能的影响,试验将小鼠随机分为正常组、模型组、桦褐孔菌多糖高剂量组、桦褐孔菌多糖中剂量组、桦褐孔菌多糖低剂量组共5个组,除正常组外,其余4组小鼠均是通过腹腔注射链脲佐菌素(STZ),1周后禁食不禁水12 h空腹血糖值≥7.8 mmol/L的成模小鼠,桦褐孔菌多糖高、中、低剂量组,分别按体重灌胃给予600,400,200 mg/kg的桦褐孔菌多糖,正常组和模型组灌胃给予等量的生理盐水,4周后禁食不禁水12 h,眼球后静脉取血测空腹血糖和三酰甘油含量;取脾脏计算脾指数;同时用流式细胞仪检测脾脏的CD_4~+/CD_8~+比值。结果表明:桦褐孔菌多糖能够在不同程度上改善糖尿病小鼠的体重和脾指数,降低其空腹血糖和三酰甘油的含量,提高脾脏的CD_4~+/CD_8~+比值。说明桦褐孔菌多糖能够降低糖尿病小鼠糖、脂水平,提高糖尿病小鼠的免疫功能。 相似文献
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《中国兽医学报》2015,(7):1103-1111
首先,测定感染小鼠最小致死量;然后,通过体内安全性试验确定各种药物治疗剂量;最后,评价5种中药对急性感染弓形虫小鼠的治疗效果,即:各药物组每只小鼠经腹腔感染25个弓形虫速殖子后4h,小鼠逐只经胃灌服剂量为25g/kg的白牛胆、垂花香薷、黄皮叶或桦褐孔菌,或10.4mg/kg蟾酥、200mg/kg磺胺间甲氧嘧啶钠,每日2次,连用5d,同时设感染不用药组和空白对照组,以小鼠存活率与平均存活时间、腹腔液中虫体数、组织病变程度以及SOD活性等指标评价药物效果。结果显示,磺胺间甲氧嘧啶钠、蟾酥、桦褐孔菌、黄皮叶、白牛胆、垂花香薷组的小鼠存活率分别为66.67%、61.11%、50.00%、5.56%、0%和0%。蟾酥、桦褐孔菌与磺胺间甲氧嘧啶钠组的小鼠平均存活时间、腹腔液中虫体数和血清SOD活性相近,三者间无显著差异(P0.05),均显著好于黄皮叶、白牛胆、垂花香薷和感染不用药组(P0.05)。在磺胺间甲氧嘧啶钠组和蟾酥组在肺脏仅出现肺泡隔毛细血管轻微充血,在肝脏和脾脏无明显病变或病变不明显;桦褐孔菌组的肝脏也无明显病变;其他治疗组的脾脏均有不同程度坏死。结果表明,蟾酥与桦褐孔菌的抗弓形虫效果与磺胺间甲氧嘧啶钠相近,白牛胆、垂花香薷和黄皮叶的抗弓形虫效果不明显。 相似文献
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《黑龙江畜牧兽医》2016,(16)
为了研究桦褐孔菌多糖对环磷酰胺(CTX)化疗小鼠免疫损伤的影响,试验将小鼠随机分为正常对照组、环磷酰胺组和桦褐孔菌多糖低、中、高剂量5个组。除正常对照组外,其余各组均皮下注射CTX 100 mg/kg;桦褐孔菌多糖低、中、高剂量组分别给予桦褐孔菌多糖溶液100,200,400 mg/kg,灌胃,正常对照组和环磷酰胺组给予等量生理盐水,1次/d,连用6 d。第7天检测各组小鼠腹腔巨噬细胞吞噬功能及血清Ig G、Ig M含量,计算免疫器官指数。结果表明:桦褐孔菌多糖可以不同程度地恢复CTX化疗小鼠的胸腺指数和脾脏指数;显著提高小鼠血清Ig G和Ig M水平(P0.05);降低CTX化疗小鼠腹腔巨噬细胞的吞噬率和吞噬指数。说明桦褐孔菌多糖能提高机体的体液免疫水平,对巨噬细胞的吞噬功能有调节作用,对CTX化疗免疫功能的损伤有一定的保护作用。 相似文献
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《现代畜牧兽医》2021,(6)
为研究黄丝藻粉对大鼠的繁殖毒性和致畸作用,研究将Wistar大鼠随机分为对照组和黄丝藻粉高、中、低(10%、5%和2%) 3个剂量组。大鼠给予黄丝藻粉10 w。结果显示,各剂量组F0、F1代孕鼠受孕率、妊娠率、产仔总数与对照组相比无显著差异(P0.05);各剂量组胎鼠窝重、胎盘重、体长、体重、死胎、吸收胎、畸形率、畸胎率等与对照组相比均无显著差异(P0.05);新生鼠出生活仔率、出生存活率、哺乳存活率、体重、饲料消耗与对照组相比无显著差异(P0.05)。除中剂量组F2鼠肾系数显著低于对照组(P0.05),其余剂量组F0、F1和F2大鼠肝、肾、脾、子宫、卵巢及睾丸等脏器系数与对照组相比均无显著差异(P0.05)。研究表明,黄丝藻粉对大鼠无明显的繁殖毒性和致畸作用。 相似文献
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为探讨桦褐孔菌粗多糖(IOCP)对急性感染弓形虫小鼠的存活时间及血清、心、肝和肺中超氧化物歧化酶(SOD)含量的影响。采用昆明系小鼠80只,随机分为IOCP组、阳性对照组(蒿甲醚)、空白对照组和阴性对照组,每组20只。用RH株弓形虫建立小鼠病理模型,观察小鼠治疗后的存活时间,以及血清、心、肝和肺中SOD含量。结果:IOCP及阳性药物均可延长感染弓形虫小鼠的存活时间;可明显提高小鼠脏器SOD活性;与阳性对照组比较差异显著(P<0.05)。结论:IOCP能延长感染弓形虫小鼠的存活时间,并具有抗氧化损伤作用。 相似文献
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Ofri R Shub N Galin Z Shemesh M Shore LS 《American journal of veterinary research》2002,63(2):159-162
OBJECTIVE: To measure intraocular pressure (IOP) and progesterone concentrations in cats and to examine their reproductive organs to determine whether reproductive status affects IOP in cats. ANIMALS:75 sexually intact domestic shorthair cats scheduled to be neutered, including 28 males, 21 females not in estrus, 13 females in estrus, and 13 pregnant females. PROCEDURES: Applanation tonometry was conducted to measure IOP and radioimmunoassay was used to determine progesterone concentrations. Reproductive organs were examined at time of surgery. RESULTS: The IOP in female cats that were in estrus was significantly higher than IOP in female cats that were not in estrus. Progesterone concentrations significantly affected IOP in pregnant cats. CONCLUSIONS AND CLINICAL RELEVANCE: In cats, IOP is affected by changes in reproductive status. Such changes should be considered when interpreting tonometry results in this species. 相似文献
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Mice were infected intraperitoneally with 10 000 tachyzoites of Toxoplasma gondii (RH) strain and, 24 h later, were treated orally for 10 days with atovaquone and azithromycin, either alone or in combination. Evaluation of the efficacy of the drugs was performed by microscopic examination of smears prepared from the organs of the mice, and by subinoculation of visceral and brain suspensions from surviving mice into healthy mice at the end of the experiments. It was found that 58%, 83% and 100% of the mice survived after administration of 75, 150 or 200 mg/kg per day of azithromycin, respectively. Moreover, 8%, 17% and 25% of the mice survived after treatment with atovaquone at 20, 50 or 100 mg/kg per day, respectively. No synergistic or additive effects of combinations of atovaquone and azithromycin were observed. However, azithromycin did not eradicate the parasite from the brain and viscera of the infected mice, whereas atovaquone at 20, 50 and 100 mg/kg per day removed the parasite from viscera and at 100 mg/kg per day eradicated the parasite from the brain of infected mice. The combinations of atovaquone and azithromycin failed to completely eradicate the parasite from the brain and viscera of infected mice. 相似文献
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本试验旨在研究酵母甘露聚糖肽（MTY）对致病性大肠杆菌（PEC）感染小鼠的保护作用。选取无特定病原体（SPF级）昆明小鼠40只,随机分为4组,分别为空白组、模型组、阳性药物组、MTY组（每组10个重复,每个重复1只小鼠）,其中,空白组和模型组灌服生理盐水,阳性药物组灌服抗生素阿莫西林,MTY组灌服酵母甘露聚糖肽。4周后,对模型组、阳性药物组、MTY组昆明小鼠进行致病性大肠杆菌（PEC）攻毒试验,通过对攻毒试验前小鼠肠道微生物分析以及PEC感染后小鼠死亡率、脏器指数、肠道组织以及各器官切片组织病理分析,同时观察体内试验过程小鼠体质变化,综合评价MTY对PEC感染小鼠的保护效果。结果表明,MTY对PEC有抑制作用。与空白组相比,MTY组小鼠肠道微生物菌群有显著性差异（P<0.05）,主要表现为有益菌丰度增加,菌群多样性增加;与模型组相比,MTY处理能有效降低小鼠感染PEC后的死亡率;MTY显著提高了小鼠胸腺指数（P<0.05）,降低脾指数（P>0.05）,并极显著降低肝指数（P<0.01）;MTY抑制了小鼠空肠杯状细胞的减少（P>0.05）;MTY抑制了小鼠肠道中MUC2及ZO-1表达下调（P<0.05）;MTY对感染PEC后的小鼠肠道、肝、脾和肺等器官均起显著的保护作用。由此可见,灌服酵母甘露聚糖肽能减轻PEC对机体的损害,进而对小鼠起保护作用。 相似文献
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中西药结合治疗弓形虫RH株感染小白鼠疗效的研究 总被引：1，自引：0，他引：1
目的观察中西药结合治疗弓形虫RH株感染小白鼠的疗效。方法用弓形虫Rh株感染昆明小鼠,设计复方中药单独治疗组,磺胺嘧啶钠单独治疗组,复方中药与磺胺嘧啶钠联合治疗组及对照组,观察对比各组小鼠存活率和存活天数。结果复方中药与磺胺嘧啶钠联合治疗组小鼠存活率93%、平均存活天数35 d,与其它各组都有显著性差异。结论中西药结合治疗感染弓形虫小鼠的疗效很好。 相似文献
15.
Mukaratirwa S Magwedere K Matenga E Foggin CM 《The Onderstepoort journal of veterinary research》2001,68(1):21-25
Forty-four Balb C mice, aged 18 weeks were infected with crocodile (Crocodylus niloticus)-derived Trichinella species. Of the infected mice, 32 were randomly divided into two groups each containing equal numbers of males and females; levamisole treated group and fenbendazole treated group. Each group was randomly subdivided into two subgroups as follows: levamisole group (subgroup 1: treated with levamisole on day 35 post infection, and subgroup 2: treated with levamisole on days 35 and 42 post infection) and fenbendazole group (subgroup 1: treated with fenbendazole on day 35 post infection and subgroup 2: treated with fenbendazole on days 35 and 42 post infection). The first subgroups treated on day 35 post infection were slaughtered on day 42 post infection and the second subgroups were treated on day 35 and day 42 post infection and slaughtered on day 49 post infection. Two female mice were infected a day after mating and were slaughtered together with the offspring on day 64 post-infection. Ten infected control mice were given 1 ml distilled water orally as placebo, and five of these were slaughtered on day 42 post infection. The results showed that the mean reproductive capacity index of this strain (RCI) in Balb C mice was 110. There was a significant reduction (P < 0.01) in larval counts in the single treatment groups (day 35) and in the double treatment groups (days 35 and 42) for both anthelmintics when compared the number of parasites in the control groups. After a single treatment, levamisole reduced the infection by 79.9% and fenbendazole by 76.7%. Following double treatments, levamisole reduced the infection by 95.5% and fenbendazole by 99.1%.There was evidence that the infected pregnant mice transmitted the parasite to their offspring. It is not certain whether the parasite was transmitted congenitally or transmammary Alternative ways of controlling the parasite in crocodile farms in Zimbabwe are discussed. 相似文献
16.
Many normally fertile stallions harbour bacteria in and on the genital organs. Many mares served by such stallions are unaffected by the bacteria to which they are thus exposed; however, some mares so exposed will become infected and diseased. Presumably, the genital defenses of such mares had been compromised. Strain differences in pathogenicity of bacteria do exist. Some mares affected with pyometra had irregular ovarian activity and some had normal ovarian cycles. In the former group, destruction of the endometrium many have prevented the production of endogenous luteolysin. The leukopenia which occurs in both groups is due to neurtropenia. 相似文献
17.
Miyamae T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(4):369-377
Pneumotropic virus invasion of parenchymal organs in mice with immunosuppressants is worth studying from an etiopathogenetic viewpoint as an animal model of a compromised host. This study analyzed the invasion by Sendai virus (SeV) of mouse organs in immunosuppressive states induced by cortisone (CO) and cyclophosphamide (CY). After subcutaneous inoculation of CO or CY, or both, in mice infected intra-nasally with SeV, the SeV invasion was investigated by detecting viremia and viral antigen in organs. SeV Nagoya strain and one treatment of CO or CY caused viremia at 18 hr or 3 d, respectively, with infection in tracheal lymph nodes, but not in untreated mice. SeV invaded hepatocytes and splenolymphocytes on days 10 and 5-10, respectively, after infection. CO or CY treatment, three times, induced viral invasion of brain tissues or serious respiratory infection, respectively, but progressive invasion of abdominal parenchymal organs was not different between treated groups. One CO and two CY treatments intensified viral invasion into many organs, maintaining seronegativity. Cerebral blood vessels had the highest incidence of viral antigen in the brain. Astroglial sheets, choroid plexuses, pia maters, and ventricular epithelia tested positive; test-positive neurons were few. SeV MN strain caused progressive invasion of the brain with gliosis and neuronophagy. Blood-brain barrier disruption was caused by virulence of the MN strain. Half the infected mice in two groups treated with CO once and CY twice succumbed to delayed hypersensitivity, suggested by cerebro-microvascular nodulation. 相似文献
18.
Swine from two herds with different histories of antibiotic exposure were fed diets containing 0 or 55 mg of chlortetracycline (CTC)/kg. One of five pigs in each herd-diet treatment group was infected orally with Escherichia coli strain BEL15R that was resistant to nalidixic acid (NA), chloramphenicol (C), streptomycin (S), sulfamethizole (TH) and tetracycline (TE). Effects of CTC on the quantity and duration of fecal shedding of E. coli BEL15R and on the transmission of strain BEL15R and its R-100 plasmid from infected pigs to uninfected pigs and chicks were determined. Quantity and duration of shedding were greater in infected antibiotic-herd pigs than in infected nonantibiotic-herd pigs. Feeding on CTC increased the duration of shedding in infected pigs from both herds. Strain BEL15R colonized and was shed in one uninfected antibiotic pig in each treatment group, but it did not colonize in any of the uninfected nonantibiotic-herd pigs or in the uninfected chicks. In vivo transfer of resistance to C, S, TH and TE occurred in the infected antibiotic-herd pigs but not in the infected nonantibiotic-herd pigs. Transfer of the R-100 plasmid occurred from the infected to the uninfected antibiotic-herd pigs and to the uninfected chicks housed near the antibiotic-herd pigs fed CTC, but not to the chicks housed with the antibiotic-herd pigs fed the control diet. No transfer of resistance occurred from the infected nonantibiotic-herd pigs fed either CTC or control diet. 相似文献
19.
为建立牛源犬新孢子虫孕鼠感染模型,深入研究牛源犬新孢子虫对孕鼠的致病作用,本试验以雌性BALB/c小鼠为试验动物,分离Vero细胞中培养的牛源犬新孢子虫速殖子,分不同剂量组腹腔接种雌性BALB/c小鼠后,与雄性BALB/c小鼠合笼,每天观察小鼠临床症状和发病情况,观察主要脏器组织的病理变化,应用PCR方法检测孕鼠脑、肝脏、脾脏等脏器组织及胎盘中犬新孢子虫Nc5基因,并测定孕鼠胎盘湿重和胎盘系数。结果显示,感染模型小鼠的最佳攻虫剂量为105个虫体;感染犬新孢子虫孕鼠先后出现精神不振、共济失调等临床症状,并有不同程度死亡;病理学观察模型小鼠脑、肝脏、脾脏等脏器组织出现充血、出血、肿大等病理变化;在模型小鼠脑、肝脏、脾脏等脏器组织及胎盘中检测到犬新孢子虫Nc5基因;随攻虫天数的增加,模型小鼠胎盘重量和胎鼠重量均不断增加,胎盘系数逐步降低,在第12、14、16天时,模型组与对照组相比,胎盘重量和胎盘系数均差异显著（P < 0.05）。本试验成功建立了牛源犬新孢子虫孕鼠感染模型,为犬新孢子虫致病机制研究奠定了基础。 相似文献