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扶芳藤内生真菌HX003抑菌活性的初步研究 总被引：1，自引：1，他引：0
[目的]探讨扶芳藤内生真菌的抑菌作用。[方法]对从扶芳藤植株中分离的内生真菌HX003进行液体培养,采用生长速率法测定获得发酵液和菌丝体的丙酮粗提物对小麦赤霉病病菌、苹果炭疽病病菌、油菜菌核病病菌、苹果轮纹病病菌、橘子胶孢炭疽病病菌的抑菌活性。[结果]将发酵液浓缩至1/10时,其对几种病原真菌的抑制率均在30%以下,对苹果轮纹病病菌的抑制率最低,而对小麦赤霉病病菌的抑制率相对较高,达27.9%。获得菌丝体的丙酮提取液对5种供试病原真菌的抑制作用较为明显,相对抑制率液体培养3 d后均达60%以上。[结论]扶芳藤内生真菌HX003的发酵液及菌丝体粗提物中具体抑菌物质有待深入研究。 相似文献
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[目的]为研制高效、低毒、低残留的新型杀菌剂提供理论依据。[方法]采用生长速率法和抑菌圈法,分别以水稻纹枯病菌等6种植物病原真菌和大肠杆菌等3种病原细菌为供试菌种,对苍耳各器官(根、茎、叶、果)提取液、植物内生真菌MG4-23的发酵液及菌丝体提取液进行抑菌活性测定。[结果]苍耳各器官提取液、植物内生真菌发酵液及菌丝体提取液均有抑菌作用。苍耳的丙酮提取液抑菌活性要好于乙醇提取液。苍耳中抑菌活性最高的器官是叶,对6种供试病原真菌的抑制率均在47.8%以上,对3种病原细菌的抑制直径均在0.5 cm以上。植物内生真菌发酵液的抑菌活性也很明显,对6种供试病原真菌的抑制率均在68.1%以上,对3种病原细菌也具有抑制性。苍耳根和植物内生真菌菌丝体提取液的抑菌活性也较明显。[结论]苍耳和植物内生真菌MG4-23均具有较大的开发利用价值。 相似文献
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3株蒲公英内生真菌的分离鉴定及抗禽类致病菌活性的初步研究 总被引：1，自引：0，他引：1
[目的]从蒲公英中筛选对禽类致病菌有较强抑制作用的植物内生真菌。[方法]采用形态学方法对分离菌株进行鉴定,以鸡致病性大肠杆菌、沙门氏菌为指示菌对分离得到内生真菌株发酵产物进行抑菌试验。[结果]从药用植物蒲公英的根和叶中分离得到3株内生真菌,分别记为PG1、PG2和PG4。初步鉴定PG1属于镰孢霉属,PG2和PG4属于卵形孢霉属。它们的发酵产物对鸡致病性大肠杆菌均有较强的抑制效果,但对沙门氏菌无抑制作用。其中,PG1的发酵液与PG2菌丝体丙酮提取液抑菌效果与2种常用禽用抗生素抑菌效果相当。[结论]蒲公英内生真菌在禽类细菌性疾病的防治中具有一定的研究价值。 相似文献
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[目的]从大叶黄杨中找到具有抗菌活性的内生真菌。[方法]采用PDA双抗培养基从大叶黄杨茎和叶中分离内生真菌,对其分别进行液体培养,将发酵后所得的发酵液用乙酸乙酯萃取处理,以大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌为试验菌株,用纸片法对分离的真菌及其次生代谢产物进行了抑菌试验。[结果]从大叶黄杨茎和叶中分离得到28株内生真菌,其中有21株内生真菌的发酵液粗提物对指示菌株有一定的抑制作用,占总分离菌株的75%。[结论]大叶黄杨内生真菌的次生代谢产物中存在着丰富的天然活性抑菌物质,可为寻找新型抗菌物质提供资源。 相似文献
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采用常规方法从南蛇藤的叶与果实中分离得到21株内生真菌,以PDB培养基对其进行液体培养,用高压蒸汽灭菌过的发酵处理液和丙酮粗提物对小麦赤霉菌、棉花枯萎菌、番茄叶霉菌等8种病原菌进行抑菌活性测定.结果表明,抑菌活性成分主要存在于丙酮粗提物中.筛选出1株抗菌谱较广的内生真菌Y9,其对番茄灰霉病菌、番茄早疫病菌、苹果炭疽病菌、西瓜枯萎病菌、苹果褐腐病菌、小麦赤霉病菌 6种病原菌有较好抑菌活性. 相似文献
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为控制猕猴桃真菌病害,本研究使用平板对峙法,从34株青蒿内生真菌中筛选出对5种常见猕猴桃病原真菌(Botryosphaeria dothidea、Collectotrichum sp.、Monilinia laxa、phoma sp.、Alternaria alternata)具有广谱高效拮抗作用的菌株,并采用菌丝生长速率法检测了拮抗菌株发酵液及其发酵液粗提物对猕猴桃病原真菌的抑菌活性。结果表明:在平板对峙试验中,21号青蒿内生真菌具有较好的抑菌活性,对猕猴桃软腐病病原菌的抑制率高达81.16%,对其他几种猕猴桃病原真菌的抑制率也均在50%以上;21号菌株发酵液在浓度为20%时,对Botryoshaeria dothidea,Collectotrichum sp.,Alternaria alternate三种病害菌的抑制率分别达到了80.26%、79.12%、45.84%;在1 mg/mL浓度下,21号菌株发酵液乙酸乙酯粗提物对Monilinia laxa、phoma sp.的抑菌活性分别达到了100%、60.45%。研究表明21号菌株发酵液乙酸乙酯粗提物能有效抑制猕猴桃病害菌株,值得进一步研究。 相似文献
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以核桃叶部分离获得的20株内生真菌为研究对象,采用抑制菌丝生长速率法测定发酵产物对8种常见植物病原真菌的抑菌效果,并对活性菌株发酵产物的乙酸乙酯萃取相进行气质联用分析(GC-MS)鉴定主要化学成分,用面积归一化法计算各成分相对含量。结果表明:15株核桃叶部内生真菌的发酵滤液对8种供试病原菌均有一定抑制作用,占内生真菌总数的75.0%。其中黑孢霉属(Nigrospora)Y4菌株对8种病原菌的抑制作用最强,抑制率均大于60%,抑菌谱广泛。Y4菌株对番茄灰霉病菌的抑制作用最大,抑制率达95.95%,其次是苹果果腐病菌(84.81%)。Y4菌株发酵产物乙酸乙酯萃取相Fr4组分经GC-MS分析共鉴定出14种化合物,占出峰物质总量的89.58%。其中哌草丹相对含量最高,达到50.46%,其他物质主要是有机酸类化合物。由此可知,核桃内生真菌具有广泛的抑菌谱,活性菌株Y4发酵产物可望研发出新型微生物源除草剂。 相似文献
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《（《农业科学与技术》）编辑部》2008,(4)
[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the antifungal activity of extracts of every organ of Xanthium sibiricum,the fermentation liquid of endophytic fungi MC4-23,and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively.[Result] The extract of every organ of Xanthium sibiricum,the fermentation liquid of endophyic fungi MC4-23,and the extract of mycelium all had antifungal activities.The acetone extracts of Xanthium sibiricum had stronger antifungal activities than the ethanol extracts of Xanthium sibiricum.The extracts of leaf have the strongest antifungal activities against fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 47.8%,and the inhibition diameters of the three pathogenic bacteria were all above 0.5 centimeter.The fermentation liquid of endophytic fungi also had strong activities against the fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 68.1%.The extracts of root of Xanthium sibiricum and the mycelium of endophytic fungi were all had antifungal activities.[Conclusion] Xanthium sibiricum and endophyic fungi MC4-23 had great value of development and utilization. 相似文献
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Preliminary Studies on Antifungal Activity of Xanthium sibiricum and the Endophytic Fungi 总被引：4，自引：0，他引：4
QI Li-yan LIU Li-li YU Ping-ru CHEN Feng-mei .Forest station of Wuxi Agroforestry Bureau Wuxi .Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Xuzhou Normal University Xuzhou 《（《农业科学与技术》）编辑部》2008,(4)
[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the antifungal activity of extracts of every organ of Xanthium sibiricum,the fermentation liquid of endophytic fungi MC4-23,and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively.[Result] The extract of every organ of Xanthium sibiricum,the fermentation liquid of endophyic fungi MC4-23,and the extract of mycelium all had antifungal activities.The acetone extracts of Xanthium sibiricum had stronger antifungal activities than the ethanol extracts of Xanthium sibiricum.The extracts of leaf have the strongest antifungal activities against fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 47.8%,and the inhibition diameters of the three pathogenic bacteria were all above 0.5 centimeter.The fermentation liquid of endophytic fungi also had strong activities against the fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 68.1%.The extracts of root of Xanthium sibiricum and the mycelium of endophytic fungi were all had antifungal activities.[Conclusion] Xanthium sibiricum and endophyic fungi MC4-23 had great value of development and utilization. 相似文献
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苍耳提取液与植物内生真菌抗菌活性研究(英文) 总被引：14，自引：2，他引：12
[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the antifungal activity of extracts of every organ of Xanthium sibiricum,the fermentation liquid of endophytic fungi MC4-23,and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively.[Result] The extract of every organ of Xanthium sibiricum,the fermentation liquid of endophyic fungi MC4-23,and the extract of mycelium all had antifungal activities.The acetone extracts of Xanthium sibiricum had stronger antifungal activities than the ethanol extracts of Xanthium sibiricum.The extracts of leaf have the strongest antifungal activities against fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 47.8%,and the inhibition diameters of the three pathogenic bacteria were all above 0.5 centimeter.The fermentation liquid of endophytic fungi also had strong activities against the fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 68.1%.The extracts of root of Xanthium sibiricum and the mycelium of endophytic fungi were all had antifungal activities.[Conclusion] Xanthium sibiricum and endophyic fungi MC4-23 had great value of development and utilization. 相似文献
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研究从广西地不容块根中分离出的DBR-12和DBR-23两株内生真菌的抑菌活性,为其在抗菌剂领域的开发利用提供依据。首先用液体培养基对内生真菌进行发酵培养,采用两相溶剂液液萃取法对发酵产物进行初步分离,然后用菌丝生长速率法和带毒平板法分别测定发酵产物对10种植物病原真菌和15种动物致病菌的抑菌活性。结果表明,DBR-12和DBR-23发酵产物乙酸乙酯萃取物对植物病原真菌均有较好的抑菌活性。DBR-12对玉米小斑病菌等6种病原真菌的抑制效果较好,有效中浓度(EC50)为0.010 4~0.079 7 g/L,其中对玉米大斑病菌的毒力最高;DBR-23对除玉米小斑病菌外的9种病原真菌抑制效果较好,EC50为0.020 6~0.174 6 g/L,其中对甘蔗凤梨病菌的毒力最高。DBR-12发酵产物正丁醇萃取物对玉米大斑病菌等5种植物病原真菌的抑制效果较好,EC50为0.107 8~0.646 9 g/L,其中对金橘砂皮病菌的毒力最高。DBR-23发酵产物乙酸乙酯萃取物对痢疾志贺氏菌等12种动物致病菌有抑菌活性,最低抑菌浓度(MIC)为1~2 g/L;DBR-12发酵产物对动物病原菌无抑菌活性。综上,广西地不容内生真菌DBR-12和DBR-23有较好的抑菌活性,在抗菌剂领域有较好的开发应用前景。 相似文献
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八角茴香抑菌活性的初步研究 总被引：4，自引：1，他引：3
采用生长速率法,测定了八角茴香不同器官的提取液对玉米小斑病菌(Helminthosporium maydis)、小麦纹枯病菌(Rizoctonia cerealis)、番茄褐斑病菌(Helminthosporium carposaprum)、棉花黄萎病菌(Verticillium dahliae)的抑菌作用,结果表明,当质量浓度为0.1 g/mL时,八角茴香不同器官的提取液对4种病原菌菌丝生长都有一定的抑制作用,但不同器官的抑菌活性不同,其中八角茴香根和枝提取液的抑菌活性较低,抑菌率均低于50%,果皮和叶提取液的抑菌活性较高,抑菌率均高于50%。 相似文献
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以花椒4种不同溶剂的提取物为供试药剂,采用生长速率法测定其对水稻纹枯病菌(Rhi-zoctonia solani)、番茄早疫病菌(Alternaria solani)、大葱紫斑病菌(Alternaria porri)和棉花枯萎病菌(Fusarium oxysporumf.sp.vasinfectum)的抑制作用,以期探索提取花椒抑菌活性物质的最佳溶剂。结果表明,花椒4种不同溶剂的提取物对4种植物病原菌菌丝生长都有一定的抑制作用,其中二甲苯提取物的抑菌活性相对较强,当用量为0.5mL时,其对水稻纹枯病菌、番茄早疫病菌、棉花枯萎病菌和大葱紫斑病菌的抑菌率分别为80.0%、78.7%、73.3%和23.2%;花椒乙醇和丙酮提取物的抑菌活性次之;甲醇提取物的抑菌活性相对较弱,当用量为0.5mL时,其对水稻纹枯病菌、番茄早疫病菌、大葱紫斑病菌和棉花枯萎病菌的抑菌率分别为56.1%、33.0%、23.4%和24.9%。因此,提取花椒抑菌活性物质的最佳溶剂是二甲苯。 相似文献