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1.
Inhibition of nitrification as a mitigation tool to abate nitrogen (N) losses and improve N use efficiency (NUE) is a promising technology. Nitrification inhibitor (dicyandiamide, DCD) was evaluated in two consecutive wheat-maize rotations (2015–2017), with two different N fertilizer levels applied in wheat (160, 220 kg N ha?1) and maize (180, 280 kg N ha?1). More NH4+-N contents (101% and 102% in wheat and 74% and 73% in maize) and less NO3-N contents (37% and 43% in wheat and 46% and 57% in maize) were observed at both N levels treated with DCD compared to without DCD. Higher pH, lower EC and reduced NO3-N accumulation were the other benefits of DCD. The NO3-N accumulation within the 0–200 cm soil profile was significantly less at both N levels with DCD (66 mg kg?1 and 121 mg kg?1) compared to without DCD (96 mg kg?1 and 169 mg kg?1). Application of DCD also improved the growth and yield in both crops. Increase in NUE from 38% to 49% in wheat and 27% to 33% in maize with DCD at higher N level was also observed. Overall, the effectiveness of DCD in retarding the nitrification process was higher in wheat than maize.  相似文献   

2.
Abstract

To determine the relationships between microbial biomass nitrogen (N), nitrate–nitrogen leaching (NO3-N leaching) and N uptake by plants, a field experiment and a soil column experiment were conducted. In the field experiment, microbial biomass N, 0.5 mol L?1 K2SO4 extractable N (extractable N), NO3-N leaching and N uptake by corn were monitored in sawdust compost (SDC: 20 Mg ha?1 containing 158 kg N ha?1 of total N [approximately 50% is easily decomposable organic N]), chemical fertilizer (CF) and no fertilizer (NF) treatments from May 2000 to September 2002. In the soil column experiment, microbial biomass N, extractable N and NO3-N leaching were monitored in soil treated with SDC (20 Mg ha?1) + rice straw (RS) at five different application rates (0, 2.5, 5, 7.5 and 10 Mg ha?1 containing 0, 15, 29, 44 and 59 kg N ha?1) and in soil treated with CF in 2001. Nitrogen was applied as (NH4)2SO4 at rates of 220 kg N ha?1 for SDC and SDC + RS treatments and at a rate of 300 kg N ha?1 for the CF treatment in both experiments. In the field experiment, microbial biomass N in the SDC treatment increased to 147 kg N ha?1 at 7 days after treatment (DAT) and was maintained at 60–70 kg N ha?1 after 30 days. Conversely, microbial biomass N in the CF treatment did not increase significantly. Extractable N in the surface soil increased immediately after treatment, but was found at lower levels in the SDC treatment compared to the CF treatment until 7 DAT. A small amount of NO3-N leaching was observed until 21 DAT and increased markedly from 27 to 42 DAT in the SDC and CF treatments. Cumulative NO3-N leaching in the CF treatment was 146 kg N ha?1, which was equal to half of the applied N, but only 53 kg N ha?1 in the SDC treatment. In contrast, there was no significant difference between N uptake by corn in the SDC and CF treatments. In the soil column experiment, microbial biomass N in the SDC + RS treatment at 7 DAT increased with increased RS application. Conversely, extractable N at 7 DAT and cumulative NO3-N leaching until 42 DAT decreased with increased RS application. In both experiments, microbial biomass N was negatively correlated with extractable N at 7 DAT and cumulative NO3-N leaching until 42 DAT, and extractable N was positively correlated with cumulative NO3-N leaching. We concluded that microbial biomass N formation in the surface soil decreased extractable N and, consequently, contributed to decreasing NO3-N leaching without impacting negatively on N uptake by plants.  相似文献   

3.
The effects of nitrogen (N) forms (ammonium- or nitrate-N) on plant growth under salinity stress [150 mmol sodium chloride (NaCl)] were studied in hydroponically cultured cotton. Net fluxes of sodium (Na+), ammonium (NH4+), and nitrate (NO3?) were also determined using the Non-Invasive Micro-Test Technology. Plant growth was impaired under salinity stress, but nitrate-fed plants were less sensitive to salinity than ammonium-fed plants due mainly to superior root growth by the nitrate-fed plants. The root length, root surface area, root volume, and root viability of seedlings treated with NO3-N were greater than those treated with NH4-N with or without salinity stress. Under salinity stress, the Na+ content of seedlings treated with NO3-N was lower than that in seedlings treated with NH4-N owing to higher root Na+ efflux. A lower net NO3? efflux was observed in roots of nitrate-fed plants relative to the net NH4+ efflux from roots of ammonium-fed plants. This resulted in much more nitrogen accumulation in different tissues, especially in leaves, thereby enhancing photosynthesis in nitrate-fed plants under salinity stress. Nitrate-N is superior to ammonium-N based on nitrogen uptake and cotton growth under salinity stress.  相似文献   

4.
Plant nitrogen (N) uptake, growth, and N use efficiency may be affected by N form (NO3 or NH4 +) available to the root. The objectives of this study were to determine the effect of mixed N form on dry matter production and partitioning, N uptake, and biomass N use efficiency defined as total dry matter produced per unit plant N (NUE1) in U.S. and tropical grain sorghums [Sorghum bicolor (L.) Moench]. The U.S. derived genotype CK 60 and three tropical genotypes, Malisor‐7, M 35–1, and S 34, were evaluated in a greenhouse trial using three nutrient solutions differing in their NO3 /NH4 + ratio (100/0, 75/25, 50/50). Shoot and root biomass, N accumulation, and NUE, were determined at 10‐leaf and boot stages. Averaged over all genotypes, shoot and root biomass decreased when NH4 + concentration was increased in the solution. Shoot biomass was reduced by 11% for 75/25 and 26% for 50/50 ratios, as compared to 100/0 NO3 /NH4 +. Similarly, root biomass reduction was about 34% and 45% for the same ratios, respectively. Increasing NH4 + concentration also altered biomass partitioning between shoot and root as indicated by decreasing root/shoot ratio. Total plant N content and NUE1 were also reduced by mixed N source. Marked genotypic variability was found for tolerance to higher rates of NH4 +. The tropical line M 35–1 was well adapted to either NO3 as a sole source, or to an N source containing high amounts of NH4 +. Such a characteristic may exist in some exotic lines and may be used to improve genotypes which do not do well in excessively wet soil conditions where N uptake can be reduced.  相似文献   

5.
ABSTRACT

Understanding how plants use of various nitrogen (N) sources is important for improving plant N use efficiency in organic farming systems. This study investigated the effects of farming management practices (organic and conventional) on pakchoi short-term uptake of glycine (Gly), nitrate (NO3 ?) and ammonium (NH4 +) under two N level conditions. Results showed that plant N uptake rates and N contributions from the three N forms in the low N (0.15 μg N g?1 dry soil) treatment did not significantly differ between the organic and conventional soils, except the significantly greater Gly contribution in organic soil at 24 h after tracer addition. Under high N (15 μg N g?1 dry soil) conditions, the N uptake rates, uptake efficiencies, and N contributions of Gly and NH4 +-N were significantly greater in pakchoi cultivated in the organic soil compared to conventional soil, whereas the N uptake rates and N contributions from NO3 -N decreased in pakchoi cultivated in the organic soil. The greater Gly-N uptake in plants grown in high-N treated organic soil may be related to the greater gross N transformation, Gly turnover rate and the increased expression of an amino acid transporter gene BcLHT1. Intact Gly contributed at most 6% to Gly-derived N at 24 h after tracer additions, which accounting for about 1.24% of the total N uptake in organic soil. Our study suggested that Gly-N and other organic source N might serve as a more important compensatory N source for plants in organic farming.  相似文献   

6.
Excessive nitrogen (N) fertilizer application is common in the central Zhejiang Province area, China. A three-year (2009–11) experiment was conducted to determine the optimum N application rate for this area by studying the effects of various N rates on rice (Oryza sativa L.) yield, N-use efficiency (NUE), and quality of paddy field water. Results showed that no significant yield differences were observed under N rates from 180 to 315 kg ha?1. The NUE could be improved by reducing N application rates without significantly decreasing yield. Due to high ammonia (NH4+-N) and nitrate (NO3N) concentrations, 5–7 days after N application was a critical stage for reducing N pollution. The N rate for the greatest yield was 176 kg ha?1, accounting for 65 percent of the conventional N rate (270 kg ha?1). The N-rate reduction in this area may be necessary for maintaining high yield, improving NUE, and reducing environmental pollution.  相似文献   

7.
Herbage growth and nitrogen (N) use efficiency in grazed or mown grasslands are generally low, associated mostly with poor response to fertilizer N. The aim of the present investigation was to examine the short-term response of grass to fertilizer N with respect to herbage yield and nitrogen use efficiency (NUE) in order to provide a better basis for improving the efficient use of fertilizer N in grassland ecosystems. Both NO3 ? and NH4 + sources of N were applied to an established grass sward with three moisture levels, i.e., natural conditions (63% water-filled pore space, WFPS), near field capacity level (71% WFPS), and slightly wetter than field capacity (84% WFPS). Herbage yield, i.e., dry matter (DM), N uptake, N recovery efficiency, yield efficiency, and physiological efficiency were determined over a 7–28 d period. Addition of N fertilizers significantly increased the herbage yield and N uptake of grass sward over that of the control. In the plots where NO3 ??N was added as the N source, DM yield was between 1760–1870 kg ha?1, N recovery efficiency was between 24%–43%, and yield and physiological efficiency were in the range of 2.1–3.2 and 6.4–8.8 kg DM kg? 1 N, respectively. In NH4 +?N added plots, the DM yield was between 3190–3700 kg ha? 1, N recovery efficiency was between 39%–48% while yield and physiological efficiency were in the range of 3.5–5.6 and 9.0–11.6 kg DM kg? 1 N, respectively. Results indicated that total DM yield, N uptake, and NUE depend on the source of N and the level of moisture in the field. Assimilation of N is also affected by the stage of plant development after N fertilization. About 50%–54% of applied N was recovered in the initial 14 and 21 d after fertilizer application and thereafter translocation of N slowed. A fall in herbage production and minimal response to N fertilizer has been observed at 84% WFPS, while the maximum herbage yield and N recovery efficiency was recorded in soil near or below field capacity. The grass sward with added NH4 +?N produced a larger yield and had higher NUE relative to the sward with NO3 ??N. Results confirm that applied N was not utilized efficiently by grass sward and a decrease in N uptake and its utilization seem to be the key factors responsible for the poor herbage productivity often observed in pastoral agriculture. These results suggest that both moisture and N source have a substantial effect on herbage yield and N utilization by plants and therefore should be considered for efficient management of N fertilization and recommendations for grass sward.  相似文献   

8.
In this paper, the uptake kinetics of various nitrogens (nitrate (NO3?), ammonium (NH4+), urea, amino acid) by Chinese kale (Brassica oleracea L. var. Bailey) were studied under hydroponic condition. The results indicated that the uptake kinetics of organic and inorganic nitrogen (N) by Chinese kale conform to the Michaelis–Menten equation, and the maximum uptake rate (Vmax) and affinity index (1/Km) showed nitrate (NO3N) > ammonium (NH4+-N) > urea-N > Gly-N, with significant differences between treatments (p < 0.05). Adding different types of N to NO3? nutrient solution had little impact on its affinity, but significantly decreased the NO3? Vmax, which showed NO3N > NO3? + NH4+ > NO3? + urea > NO3? + Gly. Chinese kale preferred inorganic N to organic N, with NO3? preceding NH4+. Adding organic and NH4+ N to nutrient solution reduced the NO3? uptake capacity by the plant.  相似文献   

9.
ABSTRACT

Plant nitrogen (N)-acquisition strategy affects soil N availability, community structure, and vegetation productivity. Cultivated grasslands are widely established to improve degraded pastures, but little information is available to evaluate the link between N uptake preference and forage crop biomass. Here an in-situ 15N labeling experiment was conducted in the four cultivated grasslands of Inner Mongolia, including two dicots (Medicago sativa and Brassica campestris) and two monocots (Bromus inermis and Leymus chinensis). Plant N uptake rate, shoot- and root biomass, and concentrations of soil inorganic-N and microbial biomass-N were measured. The results showed that the root/shoot ratios of the dicots were 2.6 to 16.4 fold those of the monocots. The shoot N concentrations of the dicots or legumes were 40.6% to 165% higher than those of the monocots or non-legumes. The four forage crops in the cultivated grassland preferred to uptake more NO3?-N than NH4+-N regardless of growth stages, and the NH4+/NO3? uptake ratios were significantly lower in the non-legumes than in the legumes (p < 0.05). Significant differences in the NH4+-N rather than NO3?-N uptake rate were observed among the four forages, related to plant functional types and growth stages. The NH4+ uptake rate in the perennial forages exponentially decreased with the increases in shoot-, root biomass, and root/shoot ratio. Also, the plant NH4+/NO3? uptake ratio was positively correlated with soil NH4+/NO3? ratio. Our results suggest that the major forage crops prefer to absorb soil NO3?-N, depending on soil inorganic N composition and belowground C allocation. The preferential uptake of NO3?-N by forages indicates that nitrate-N fertilizer could have a higher promotion on productivity than ammonium-N fertilizer in the semi-arid cultivated grassland.  相似文献   

10.
In pursuit of highyield, blind application of a large number of nitrogen fertilizer in tea plantation has not only seriously affected the quality of tea, but also resulted in environmental degradation. This study aimed to screenvarieties of tea cultivars with nitrogen efficiency. Twenty five different tea cultivars were collected and transferred into pot planting experiments to study their changes of total nitrogen and nitrogen utilization efficiency under absence nitrogen supply [0 mmol L?1 ammonium nitrate (NH4NO3)] and normal nitrogen (6 mmol L?1NH4NO3). Comparison with normal nitrogen supply, the biomass, total uptake of nitrogenand nitrogen utilization efficiencyof most tea cultivars under nitrogen deficiency tended to decrease. The cluster analysis of relative indices (RI) of biomass, total nitrogen contents, and nitrogen utilization efficiencyindicates that the Fudingdahao (#3), Zimudan (#23), and Huangdan (#24) tea cultivars are not sensitive to nitrogen deficiency and it can greatly resist with low nitrogen supply.  相似文献   

11.
Ammonium(NH+4) is the main nitrogen(N) form for rice crops, while NH+4near the root surface can be oxidized to nitrate(NO-3)by NH+4-oxidizing bacteria. Nitrate can be accumulated within rice tissues and reused when N supply is insufficient. We compared the remobilization of NO-3stored in the tissue and vacuolar between two rice(Oryza sativa L.) cultivars, Yangdao 6(YD6, indica)with a high N use efficiency(NUE) and Wuyujing 3(WYJ3, japonica) with a low NUE and measured the uptake of NO-3, expression of nitrate reductase(NR), NO-3transporter genes(NRTs), and NR activity after 4 d of N starvation following 7-d cultivation in a solution containing 2.86 mmol L-1NO-3. The results showed that both tissue NO-3concentration and vacuolar NO-3activity were higher in YD6 than WYJ3 under N starvation. YD6 showed a 2- to 3-fold higher expression of OsNRT2.1 in roots on the 1st and 4th day of N starvation and had significantly higher values of NO-3uptake(maximum uptake velocity, Vmax) than the cultivar WYJ3.Furthermore, YD6 had significantly higher leaf and root maximum NR activity(NRAmax) and actual NR activity(NRAact) as well as stronger root expression of the two NR genes after the 1st day of N starvation. There were no significant differences in NRAmax and NRAact between the two rice cultivars on the 4th day of N starvation. The results suggested that YD6 had stronger NRA under N starvation, which might result in better NO-3re-utilization from the vacuole, and higher capacity for NO-3uptake and use, potentially explaining the higher NUE of YD6 compared with WYJ3.  相似文献   

12.
在河北衡水潮土上进行田间试验,以当地习惯高氮用量(小麦季施N 300 kg/hm2,玉米季施N 240 kg/hm2)为对照,研究冬小麦-夏玉米轮作体系中减少氮肥用量对玉米季植株生长、氮素吸收及根际土壤中无机氮与微生物量氮的影响。结果表明,两季作物氮肥施用量减少25%和40%,对玉米产量、生物量及植株体内氮累积量未产生明显影响,氮肥利用率提高。不同氮肥施用量对根际和非根际土壤铵态氮含量的影响不显著;减少氮肥施用量,对玉米根际土壤硝态氮含量也没有明显影响。在玉米苗期、抽雄期和成熟期,习惯高施氮量处理的非根际土壤硝态氮含量较高,其中抽雄期,非根际土壤硝态氮含量较氮肥减施40%用量处理高出近一倍,但非根际土壤微生物量氮水平含量明显降低。氮肥减施未影响根际土壤微生物量碳、氮含量,反而增加了非根际土壤微生物量碳、氮水平。在高肥力的潮土上,冬小麦/夏玉米轮作体系中适当减施氮肥并未影响玉米根际土壤氮素水平,可保证玉米稳产,实现减氮增效。  相似文献   

13.
Ammonium and nitrate are the major forms of nitrogen (N) present in tropical soils. An experiment was conducted to assess the influence of nitrate and ammonium forms (NO3?, NH4+, and mix of NO3? + NH4+), and levels (1.5–12.0 mM) of N on the growth and nutrition of cacao (Theobroma cacao L). Growth parameters were significantly influenced by N forms, and nitrogen supplied as NH4+ proved better for the growth of cacao compared with NO3? form and mixtures of these two forms. Irrespective of the forms of N, levels of N had no significant effect on plant growth parameters. Nutrient efficiency ratios (NERs) (shoot dry matter produced per unit of nutrient uptake) for macronutrients were sulfur>phosphorus>calcium>magnesium>nitrogen>potassium (S>P>Ca>Mg>N>K) and for micronutrients NERs were in the order of copper>boron>zinc>iron>manganese (Cu>B>Zn>Fe>Mn).  相似文献   

14.
为解决区域土壤质地类型针对性氮肥施用问题,在轻壤土和黏壤土上分别设置不施氮肥,氮肥基追比3∶7,4∶6,5∶5,6∶4和7∶3处理,研究小麦产量、水氮利用效率以及土壤含水量、贮水量、NH_4~+-N、NO_3~--N动态变化规律。结果表明:轻壤质土壤氮肥基追比4∶6的处理小麦产量、水分利用效率、氮肥生产效率最高分别为8 265.3 kg/hm~2,27.6 kg/(hm~2·mm),34.4 kg/kg。黏壤质土壤氮肥基追比5∶5的处理小麦产量、水分利用效率、氮肥生产效率最高分别为8 363.2 kg/hm~2,28.3 kg/(hm~2·mm),34.8 kg/kg。小麦不同生育期各土层含水量垂直分布变化较大,轻壤质土壤含水量在9.3%~26.2%,而黏壤质为9.7%~27.6%;小麦全生育期内土壤贮水量呈先升高后降低趋势,黏壤质土壤贮水量高于轻壤质。氮素追施量越多土壤表层NH_4~+-N与NO_3~--N含量越高,且随土层加深土壤NH_4~+-N与NO_3~--N含量降低,受降水影响轻壤质土壤NH_4~+-N与NO_3~--N更易于向土层深处淋溶,成熟期黏壤质各土层的NH_4~+-N和NO_3~--N含量均多于轻壤质。说明黏壤质土壤保水保氮肥能力强于轻壤质,氮肥基追比可以适当增加。  相似文献   

15.
Maximizing nitrogen use efficiency (NUE) involves synchronizing the interplay between nitrogen preferential crops and the nitrogen transformation pathways of soil. Biochar may benefit specific N-preference crops in relatively unsuitable soil environments; however, experimental data are lacking. This study tested eight treatments, consisting of four nitrogen treatments (N0 = control; N1 = NH4Cl; N2 = NaNO3; and N3 = 1:1 ratio of NH4+ and NO3) each with biochar applied at 0% or 2% (w/w). The results show that biochar and/or nitrogen application enhanced maize seedling biomass and NO3-based fertilizer resulted in higher seedling biomass than NH4+-based fertilizer. With the application of biochar and NH4+-based fertilizer, maize seedling biomass increased and soil NH4+-N content was significantly reduced compared with NH4Cl sole application. Correlation analysis and redundancy analysis revealed that SOC content and inorganic nitrogen content were the main factors influencing maize growth and N absorption. Biochar with or without nitrogen fertilizer (except N1 treatment) significantly increased β-1,4-glucosidase (BG) activity. Co-application treatments also resulted in higher vector length, an indicator of C limitation—the increment might add to the risk of microbial C limitation. The activity of ammonia monooxygenase (AMO), a key enzyme in nitrification, decreased with the co-application of biochar and nitrogen, suggesting the alteration of nitrogen transformation.  相似文献   

16.
利用膜进样质谱法测定不同氮肥用量下反硝化氮素损失   总被引:5,自引:2,他引:3  
王书伟  颜晓元  单军  夏永秋  汤权  林静慧 《土壤》2018,50(4):664-673
利用膜进样质谱仪(MIMS)测定了太湖流域典型稻田不同氮肥施用梯度下,土壤反硝化氮素损失量,同时也对氨挥发通量进行了观测。根据两年的田间试验结果得到:在常规施氮处理(N300)下,每年平均有54.8 kg/hm~2 N通过反硝化损失,有约54.0 kg/hm~2 N通过氨挥发损失,分别占肥料施用量的18.3%和18.0%,两者损失量相当。通过反硝化和氨挥发损失的氮素量随着氮肥用量增加而增加,田面水的NH_4~+-N、NO_3~–-N、DOC和pH浓度影响稻田土壤反硝化速率。在保产增效施氮处理(N_270)下,氮肥施用量比常规减少10%,水稻产量增加了5.5%,而通过反硝化和氨挥发损失的氮素量分别下降了1.1%和3.1%,氮肥利用率提高了约5.5%。在增施氮肥处理(N375)下,因作物产量增加使得氮肥利用率比N300增加,但通过氨挥发和反硝化的氮素损失量也最大。因此,通过综合集约优化田间管理措施,降低氮肥用量,可实现增产增效的目的。  相似文献   

17.
选取闽江福州下游段水体及河口短叶茳芏湿地土壤水作为研究对象,采用SAN++连续流动分析仪测试样品中NO-3—N,NO-2—N和NH+4—N含量,以揭示河流水体N含量的季节差异和对土壤水N含量的影响。研究结果表明:(1)闽江福州下游段秋季河流水NH+4—N和NO-3—N含量高于春季,NO-2—N含量低于春季;秋季短叶茳芏湿地土壤水NH+4—N和NO-2—N含量也明显高于春季,土壤水NO-3—N含量低于春季;春、秋季土壤水NH+4—N含量皆高于河流水,而NO-3—N和NO-2—N含量皆明显低于河流水。(2)河流水的浸淹对土壤N含量影响较大,说明河流水是湿地土壤的主要N源。(3)闽江福州下游段河流水3种形态的N含量表现为秋季大于春季,存在较明显的季节差异。(4)与2007—2008年的观测值相比较,闽江河口河流水体N含量呈大幅上升趋势,水体富营养化加剧。  相似文献   

18.
Controlled-release urea (CRU) and its placement method in rice production were investigated during 2007 and 2008 seasons. Controlled-release urea was applied at 62.5, 125, and 187.5 kg nitrogen (N) ha?1, and the urea was 187.5 kg N ha?1. All the CRU treatments were applied to the nursery beds once, and they were brought into the paddy field during transplanting, while the urea treatment was split into three applications from the plowing to the harvest. The results showed that rice seedlings with CRUs germinated and grow well and there was no salt damage at the nursery stage. The CRU treatment with 125 kg N ha?1 had 33% less N than urea treatment (187.50 kg N ha?1), but it produced significantly higher grain and straw yields, higher total N uptake and total apparent N uptake efficiency. In addition, all the CRU treatments effectively decreased floodwater ammonium (NH4 +)-N and nitrate (NO3 ?)-N concentrations, pH, and N runoff.  相似文献   

19.
Aerobic incubations to estimate net nitrogen (N) mineralization typically involve periodic leaching of soil with 0.01 M calcium chloride (CaCl2), so as to remove mineral N that would otherwise be subject to immobilization. A study was conducted to evaluate the accuracy of leaching for analysis of exchangeable ammonium (NH4+)-N and nitrate + nitrite (NO3?+ NO2)-N, relative to conventional extractions using 2 M potassium chloride (KCl). Ten air-dried soils were used, five each from Illinois and Brazil, that had been amended with NH4+-N (1 g kg?1) and NO3-N (0.6 g kg?1). Both methods were in good agreement for inorganic N analysis of the Brazilian Oxisols, whereas leaching was significantly lower by 12–48% in recovering exchangeable NH4+-N from Illinois Alfisols, Mollisols, and Histosols. The potential for underestimating net N mineralization was confirmed by a 12-wk incubation experiment showing 9–86% of mineral N recoveries from three temperate soils as exchangeable NH4+.  相似文献   

20.
Abstract

The aim of this study was to assess the mitigating effects of lime nitrogen (calcium cyanamide) and dicyandiamide (DCD) application on nitrous oxide (N2O) emissions from fields of green tea [Camellia sinensis (L.) Kuntze]. The study was conducted in experimental tea fields in which the fertilizer application rate was 544 kg nitrogen (N) ha?1 yr?1 for 2 years. The mean cumulative N2O flux from the soil between the canopies of tea plants for 2 years was 7.1 ± 0.9 kg N ha?1 yr?1 in control plots. The cumulative N2O flux in the plots supplemented with lime nitrogen was 3.5 ± 0.1 kgN ha?1, approximately 51% lower than that in control plots. This reduction was due to the inhibition of nitrification by DCD, which was produced from the lime nitrogen. In addition, the increase in soil pH by lime in the lime nitrogen may also be another reason for the decreased N2O emissions from soil in LN plots. Meanwhile, the cumulative N2O flux in DCD plots was not significantly different from that in control plots. The seasonal variability in N2O emissions in DCD plots differed from that in control plots and application of DCD sometimes increased N2O emissions from tea field soil. The nitrification inhibition effect of lime nitrogen and DCD helped to delay nitrification of ammonium-nitrogen (NH4+-N), leading to high NH4+-N concentrations and a high ratio of NH4+-N /nitrate-nitrogen (NO3-N) in the soil. The inhibitors delayed the formation of NO3-N in soil. N uptake by tea plants was almost the same among all three treatments.  相似文献   

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