共查询到18条相似文献，搜索用时 234 毫秒
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抗冻蛋白是一类能控制冰晶生长和抑制冰晶之间发生重结晶的蛋白质,并具有两种明显不同的活性--热滞活性和重结晶抑制活性.植物抗冻蛋白的抗冻特性是低热滞活性和高重结晶抑制活性.植物抗冻蛋白虽然在DNA和氨基酸水平上具有较大的差异,几乎没有同源性,但却拥有相似的高级结构,冰晶结合位点也有一定的相似性,而且都可以通过"表面互补"模型较好地解释它们与冰晶之间的相互作用.全面介绍了植物抗冻蛋白的种类及特性,以及它们的高级结构和冰晶结合位点. 相似文献
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以油茶优良无性系湘林1号和湘林4号近成熟种子为材料构建cDNA文库,随机挑取2327个克隆进行3′端测序,并将所得序列与核酸数据库进行同源性比较.发现16种58条与油茶种子胚胎发育及种子成熟相关的基因序列,这些序列与核酸数据库中其他物种相关序列有很高或较高的同源性.其中,脱落酸应答蛋白、成熟控制蛋白、翻译控制肿瘤蛋白、胚胎特化蛋白、乙烯应答因子包含域蛋白等基因序列为高丰度表达;种子成熟蛋白、细胞分化蛋白、乙烯应答因子、脱水素等编码基因属中等丰度表达;而成熟相关蛋白、胚珠发育蛋白、伸展蛋白、生长控制因子、休眠相关蛋白、衰老相关蛋白和脱水应答蛋白等基因只检测到1条序列,属低丰度表达.这些结果为揭示油茶种子油脂转化过程中的基因表达和生理过程提供了科学依据. 相似文献
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以油茶优良无性系湘林1号和湘林4号近成熟种子为材料构建cDNA文库,随机挑取2327个克隆进行3′端测序,并将所得序列与核酸数据库进行同源性比较.结果显示,有88条油脂储藏蛋白相关基因和3种61条储藏蛋白基因表达,并具有典型的“时、空”特点.这些结果为揭示近成熟时期油茶种子中储藏蛋白特异表达的丰度和生理情况提供了科学依据. 相似文献
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将胡萝卜抗冻蛋白在大肠杆菌中进行表达后,对获得的包涵体进行了初步的洗涤纯化.分别采用HPLC和N ativePAGE法对其进行最终的纯化.结果表明,两种方法都能获得高度纯化的目的蛋白,而且耗时都很少.但N ative PAGE法更为经济,且可以同时进行多个样品的纯化,更适用于一般实验室. 相似文献
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以葡萄桐近成熟种子为材料,根据油桐转录组数据库中已知部分烯脂酰CoA还原酶(enoyl-CoA reductase,ECR)序列,利用RACE克隆技术,克隆得油桐烯脂酰CoA还原酶的全长c DNA序列,命名为VfECR,并对该基因的核苷酸序列及其相应氨基酸序列的理化性质、疏水性/亲水性、跨膜结构域、蛋白质的二、三级结构、亲缘关系进行分析。结果表明:该基因全长1 154 bp,含有一个933 bp的开放阅读框,编码310个氨基酸,属于不稳定的亲水性蛋白,含有6个明显的跨膜结构域,且与陆地棉ECR蛋白的同源性最高,亲缘关系最为接近。同时利用RT-qPCR对VfECR基因的组织特异性和种子发育过程中的表达模式进行了分析,发现油桐Vf ECR基因主要在种仁中表达,在根、茎、叶等组织中表达量较少;在种子发育过程中,近成熟(8月、9月)到成熟种子(10月)中的表达量要明显高于未成熟种子(6月、7月)。 相似文献
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《西部林业科学》2016,(2)
牻牛儿基牻牛儿基焦磷酸合成酶(GGPPS)是萜烯类物质合成的关键酶之一,本研究通过同源序列检索分析设计引物,从马尾松幼苗针叶组织中克隆到其GGPPS基因,命名为Pma GGPPS,该基因c DNA长1 143bp,开放阅读区编码380个氨基酸。Pma GGPPS编码的蛋白质序列分析显示具有Trans IPPS结构域及FARM、SARM两个富含天冬氨酸区域,序列特点与GGPPS蛋白的酶学功能相符。同源性分析结果显示Pma GGPPS核酸序列与其他两种松属植物GGPPS基因同源性达到99%以上,Pma GGPPS编码蛋白与挪威云杉等植物中GGPPS蛋白同源性也在69%以上。对Pma GGPPS编码蛋白进行理化性质及结构的生物信息学分析,结果显示该蛋白为一种无信号肽、无跨膜区的碱性蛋白质,二级结构由16段α-螺旋组成,三级结构同源建模显示与欧薄荷的异聚牻牛儿基焦磷酸合酶大亚基同源性最高。系统进化树分析显示该蛋白与红豆杉、银杏亲缘关系较近。本研究中克隆得到Pma GGPPS基因为深入研究其在松脂合成的影响奠定了基础,同时根据GGPPS基因设计荧光定量引物并优化反应条件,为下一步研究基因表达水平与产脂力关系提供了理论依据和技术参考。 相似文献
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《绿色科技》2017,(20)
指出了蓝藻水华聚集引起水体环境恶化,人们可利用生物浮床来净化此类水体,黑麦草是供选择的浮床生物之一。在实验室中模拟生态浮床,探索了5种不同初始浓度的铜绿微囊藻接种后,生长于浮床上的黑麦草的生长情况及其生理响应,并比较了水体净化效果。结果表明:接种处理组的黑麦草的根长、鲜重与空白相比,都呈现增加趋势,但当蓝藻初始接种量达0.278g/L时,生长情况较其他接种组变差;黑麦草叶片可溶性蛋白、MDA含量在大部分接种组都增加。此外,不接种蓝藻时,黑麦草能十分有效吸收水中的磷;初始接种量低于0.248g/L时,随着接种量的增加,水体的TP降低了78%~92%,CODCr却不断升高;超过0.248g/L的接种处理组中,实验期间TP减少率超过90%,但CODCr达到最大值。因此,在利用黑麦草净化富营养水体时,为更好地发挥其水体净化功能,应将种植时间提前,避免蓝藻水华的严重聚集。 相似文献
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Dai Huanqin Lu Cunfu* Zhang Hui Zhang Xujia College of Biological Sciences Biotechnology Beijing Forestry University Beijing P. R. China Institute of Biophysics Chinese Academy of Sciences Beijing P. R. China 《中国林学(英文版)》2003,5(4)
Populus euphratica Olive is the only tree species that can grow in the saline land and also survive cold winters in northwest China, and it plays a very important role in stabilizing the vulnerable ecosystem there. A cell suspension culture was initiated from callus derived from plantlets of Populus euphratica. Cold acclimation was induced (LT50 of-17.5 ℃) in cell suspension at 4-5 ℃ in the dark for 30 days and the freezing tolerance increased from LT50 of-12.5 ℃ in nonacclimated cells to LT50 of-17.5 ℃ in cold-acclimated cells. Microvacuolation, cytoplasmic augmentation and accumulation of starch granules were observed in cells that were cold-acclimated by exposure to low temperatures. Several qualitative and quantitative changes in proteins were noted during cold acclimation. Antibodies to carrot extracellular (apoplastic) 36 kD antifreeze protein did not cross react on immunoelectroblots with extracellular proteins in cell suspension culture medium of Populus euphratica, indicating no common epitopes in the carrot 36 kD antifreeze protein and P euphratica extracellular proteins. The relationship of these changes to cold acclimation in Populus euphratica cell cultures was discussed. 相似文献
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Xu Wen-li Liu Mei-qin Shen Xin Lu Cun-fu 《中国林学(英文版)》2005,7(4):11-15
Antifreeze proteins （AFPs） enable organisms to survive under cold conditions, and have great potential in improving cold tolerance of cold-sensitive plants, In order to determine whether expression of the carrot 36 kD antifreeze protein gene confers improved cold-resistant properties to plant tissues, we tried to obtain transgenic tobacco plants which expressed the antifreeze protein. Cold, salt, and drought induced promoter Prd29A was cloned using PCR from Arabidopsis. Two plant expression vectors based on pBI121 were constructed with CaMV35S：AFP and Prd29A：AFP. Tobacco plantlets were transformed by Agrobacterium-medicated transformation. PCR and Southern blotting demonstrated that the carrot 36 kD afp gene was successfully integrated into the genomes of transformed plantlets. The expression of the afp gene in transgenic plants led to improved tolerance to cold stress. However, the use of the strong constitutive 35S cauliflower mosaic virus （CaMV） promoter to drive expression of afp also resulted in growth retardation under normal growing conditions. In contrast, the expression of afp driven by the stress-inducible Prd29A promoter from Arabidopsis gave rise to minimal effects on plant growth while providing an increased tolerance to cold stress condition （2℃）. The results demonstrated the prospect of using Prd29A-AFP transgenic plants in cold-stressed conditions that will in turn benefit agriculture. 相似文献
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Ma Xinyu Shen Xin Lu Cunfu* College of Biological Sciences Biotechnology Beijing Forestry University Beijing P. R. China 《中国林学(英文版)》2003,5(4)
The recombinant expression vectorpET43. lb-AFP, which contains full encoding region of a carrot 36 kD antifreeze protein (AFP) gene was constructed. The recombinant was transformed into expression host carrying T7 RNA polymerase gene (DE3 lysogen) and induced by 1 mmol. L-1 IPTG (isopropyl-β-D-thiogalactoside) to express 110 kD polypeptide of AFP fusion protein.The analysis of product solubility revealed that pET43. 1b-AFP was predominately soluble, and the expressed amount reached the maximum after the IPTG treatment for 3 h. 相似文献
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本研究的目的在揭示低温对紫叶矮樱生理活动的影响机理,找出最佳防寒技术措施,解决其在北方冬季栽培的技术难题,促进其在我国的生产和利用。室内实验在-25℃下,规定时间梯度,利用褐变观察法和恢复生长法测定紫叶矮樱抗寒性。室外实验分别对紫叶矮樱进行根颈培土树干裹草处理、喷防冻液处理、树干涂白处理、风障处理、未做防护处理,观察其生长萌动状况和冻伤情况。文中最终得出紫叶矮樱越冬防寒技术序列以防寒效果排序为根颈培土树干裹草技术、风障处理技术、喷防冻液技术、树干涂白技术。
相似文献
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Alleviation of dormancy in walnut kernels by moist chilling is independent from storage protein mobilization 总被引：1，自引：0，他引：1
We studied the effects of moist chilling and warming on storage protein mobilization in walnut (Juglans regia L.) kernels to assess the metabolic inhibition theory, which states that dormant seeds are unable to utilize their own food reserves and that cold conditions allow germination by activating hydrolases involved in reserve mobilization. Stratifying kernels at 5 degrees C for 40 days enhanced their germination. During both cold stratification and warm incubation of kernels, storage protein mobilization occurred in cotyledons rather than axes. Kernel amino acid concentration increased during protein mobilization, with axes of warm-incubated kernels having particularly high amino acid concentrations. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) of the soluble protein fractions from both cold-stratified and warm-incubated cotyledons revealed increased band intensities of putative glutelins (19-22 and 32-35 kDa). A very high molecular weight protease was detected by gelatin SDS-PAGE that was most active at acid to neutral pHs in imbibed, cold stratified and germinated kernels suggesting the protease(s) was synthesized earlier in the mature seeds. Thus, in dormant walnut kernels there is no block to protein mobilization, and imbibition alone is sufficient to initiate proteolysis. Catalase activity was higher in warm-incubated kernels than in cold-stratified kernels, suggesting that seed aging is hastened under warm conditions and that cold stratification in walnut kernels might involve activation of cellular repair mechanisms. 相似文献
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Low temperature is one of the major limiting environmental factors which constitutes the growth, development,productivity and distribution of plants. Over the past several years, the proteins and genes associated with freezing resistance of plants have been widely studied. The recent progress of domestic and foreign research on plant antifreeze proteins and the identification and characterization of plant antifreeze protein genes, especially on expression regulatory mechanism of plant antifreeze proteins are reviewed in this paper. Finally, some unsolved problems and the trend of research in physiological functions and gene expression regulatory mechanism of plant antifreeze proteins are discussed. 相似文献
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目前仁用杏生产中存在的最大问题是开花早,花期和幼果期易遭受晚霜、寒流的危害,引起冻花冻果现象的发生,导致减产甚至绝收。为了解决仁用杏生产上易受霜冻危害的问题,以仁用杏主栽品种‘优一’为试验材料,研究了喷施抗冻剂1,2-丙二醇、1,2-丙二醇+葡萄糖的混合液、金硅谷和灵护防冻液和水杨酸、施肥、柴堆熏烟处理对仁用杏抗抵御晚霜能力的影响情况,从中筛选出了能有效增强仁用杏抵御晚霜能力的处理方法。结果表明:在其盛果期的采果之后,每株施有机肥10~15 kg或者复合肥1.0~1.5 kg,不仅能促进仁用杏树势的生长,提高其来年的花果质量,而且能有效减轻晚霜的危害,使其在幼果期的冻果率减少50%左右;在霜冻来临之前,喷施2~3次20%~30%的1,2-丙二醇溶液与10%的葡萄糖溶液的混合液,可使其在花期或幼果期能抵抗5~8 h以上、-5℃以下的低温,能使其在幼果期的抗冻率提高80%以上,且对仁用杏花朵(花瓣、雌蕊、子房、雄蕊)和幼果均不会造成伤害,也不影响仁用杏的坐果率。试验结果表明,这两种方法操作简单,低成本,农民容易掌握,易于生产中大范围内推广。 相似文献
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【目的】 TATA框结合蛋白相关因子TAF10作为基本转录因子之一,在生长发育和胁迫响应过程中发挥着广泛的、重要的生物学作用。对蜡梅中TAF10同源基因 CpTAF10的克隆与功能分析,有利于丰富对植物TAFs基因功能的认识,并为解析蜡梅抗逆形成的转录调节机理提供新的理论依据。【方法】以转录组数据库中获得的蜡梅TAFs家族基因序列,克隆得到 CpTAF10基因的cDNA序列,并对其编码蛋白进行序列特征和进化树分析。采用实时荧光定量PCR 技术分析 CpTAF10基因在蜡梅不同组织及花期中的表达特性,以及高温、低温、盐胁迫及ABA处理后的表达变化。同时,构建 CpTAF10基因的过表达载体,采用花序侵染法进行拟南芥遗传转化,对拟南芥转基因纯合株系进行表型观察和胁迫耐性分析。【结果】获得的 CpTAF10基因 cDNA序列为712 bp,包含405 bp的开放阅读框(ORF),编码134个氨基酸,蛋白理论分子量为15.21 kDa,预测的等电点pI值为5.19。CpTAF10蛋白序列与其他植物同源序列具有较高的同源性,蛋白多序列比对显示CpTAF10蛋白属于TAF10同源蛋白,并含有组蛋白折叠结构域。表达特性分析结果发现,CpTAF10基因在蜡梅的根、茎、子叶、幼叶、成熟叶和花6个不同组织中均有不同程度的表达,其中,在成熟叶中的表达量最高。 CpTAF10在蜡梅花朵的不同花期中,呈现出波动的表达模式,在衰老期表达量最高。在低温、盐胁迫和ABA处理的蜡梅叶片中均能被诱导表达,但其表达变化各不相同。在拟南芥中过表达 CpTAF10基因可提高盐胁迫下拟南芥种子的萌发率,相对于野生型植株,转基因植株的主根和侧根在盐胁迫下均表现出一定的生长优势。【结论】 CpTAF10基因能在低温、盐胁迫和ABA处理后诱导表达,可能参与蜡梅逆境胁迫耐性的分子调控。在拟南芥中过表达 CpTAF10基因显著提高了转基因拟南芥的萌芽率及主根和侧根的生长优势,在一定程度上可增强植物的盐胁迫耐性。 相似文献