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《黑龙江畜牧兽医》2019,(24)
为了对从临床患病犬皮肤采集的样品进行分离菌鉴定,并了解犬源伪中间型葡萄球菌(Staphylococcus pseudintermedius)的生物被膜形成能力,试验对采集的临床患犬的皮肤样品通过染色镜检、触酶试验、血浆凝固酶试验、耐热核酸酶试验进行鉴定,并对分离菌进行药敏试验,对16S rRNA基因、凝固酶(Coagulase)基因、MecA基因及生物被膜主要基因(ica、sarA)进行PCR扩增,同时对Coagulase、MecA基因的PCR扩增产物测序并进行BLAST比对分析。结晶紫染色定量法检测分离菌的生物被膜形成能力,扫描电镜(SEM)观察生物被膜的形态结构。结果表明:染色镜检、触酶试验、血浆凝固酶试验初步确定分离菌为葡萄球菌,耐热核酸酶试验初步确定为伪中间型葡萄球菌。分离菌对红霉素、头孢哌酮、哌拉西林等抗菌药物表现为中介或耐药,对多西环素、四环素、米诺环素等敏感。16S rRNA基因、Coagulase基因、MecA基因及生物被膜主要基因(ica、sarA)PCR扩增结果均为阳性,16S rRNA基因、MecA基因扩增产物测序结果表明,分离菌为耐甲氧西林伪中间型葡萄球菌。结晶紫染色定量法检测结果表明,该分离菌可形成中等黏附。电镜检测可见菌体呈球形,菌体聚集成簇,细菌间有黏液样物质,且菌体表面被大量丝状黏性物质包裹,即菌体分泌的大量胞外物质。说明本试验分离得到耐甲氧西林伪中间型葡萄球菌,该分离菌具有生物被膜形成能力。 相似文献
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为确定水貂黏质沙雷氏菌的耐药状况、致病性及其遗传特征,本研究从黑龙江省患病死亡水貂中分离得到3株细菌,根据其形态特征、培养特性、生化试验及16S rRNA序列测定对分离菌进行鉴定;随后用K-B法测试分离株对常见的24种抗菌药的敏感性,并进行了致病性试验和遗传进化分析。结果显示,分离菌为黏质沙雷氏菌,对多种常用抗菌药物具有高度耐药性;可引起试验组小鼠100%死亡;分离株与来自活性污泥与火山堆积物环境的菌株遗传亲缘性最近。表明黑龙江省水貂中存在黏质沙雷氏菌的感染,分离株耐药现象较严重并具有很强的致病性,同时提示该菌株可能来源于环境中。本试验从水貂中分离得到致病性黏质沙雷氏菌为首次报道,研究结果可作为该菌感染水貂导致相关疾病的检测、鉴别和预防依据。 相似文献
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2013~2016年山东省、河北省、黑龙江省、吉林省的部分水貂养殖场发生了严重的出血性肺炎,导致水貂大量死亡,为了客观评估水貂出血性肺炎的危害及为综合防控提供参考依据,试验对423个水貂病例样本进行分离鉴定并且对所分离的细菌进行了形态特征观察、16SrRNA基因测序、血清型鉴定、小鼠毒力试验测定、生化试验鉴定及药敏试验测定。结果表明:423个样本中分离到68株肺炎克雷伯氏菌,其中最为流行的血清型为K2型(35株);并筛选出4株菌株对小鼠有很强毒力性;生化试验结果表明同一血清型的不同菌株之间也存在明显差异;药敏试验结果表明,4株K2型肺炎克雷伯氏菌强毒菌株对氧氟沙星、阿米卡星2种抗生素均为敏感或介于中间;对强力霉素、环丙沙星等8种抗生素均耐药;对诺氟沙星、庆大霉素等4种抗生素均为耐药或介于中间。 相似文献
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猪源缓慢葡萄球菌的分离鉴定及致病性研究 总被引：1，自引：0，他引：1
某猪场发生一种高发病率、高死亡率的传染病。本研究从该场濒死猪只的心脏血液及肺脏中分离出1株细菌,经镜检、菌落形态观察、培养特性观察及法国生物梅里埃API鉴定系统鉴定,确定该分离菌株为缓慢葡萄球菌。药敏试验表明该菌对多种药物具耐药性,但对氟哌酸、红霉素和强力霉素等敏感;致病性试验表明,分离菌可以引起实验小鼠的慢性死亡,猪只腹腔注射可复制病例,表明缓慢葡萄球菌具有致病性并很可能会对公共卫生安全具有潜在威胁。 相似文献
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《中国家禽》2021,(7)
2020年6月,潍坊临朐某养鹅场饲养的2月龄朗德鹅不断出现发病和死亡,剖检可见内脏器官、脂肪、肌肉出血。为确定发病原因,进行病原的分离鉴定。结果显示:从发病鹅内脏器官中分离到一株革兰氏阳性球菌,该分离菌对头孢拉定、米诺环素高敏;显微镜检、生化试验、微生物质谱仪检测、16 S rRNA基因测序和同源性分析确定该分离菌为凝固酶阴性的缓慢葡萄球菌。致病性试验表明,该分离菌可以引起雏鹅发病死亡,发病率为100%,死亡率为89%,死亡雏鹅以肝脏肿大出血、肺脏出血等内脏器官出血为主要病理变化,与自然发病一致。研究首次在鹅上分离出缓慢葡萄球菌,为鹅的缓慢葡萄球菌病的防治和合理用药提供了依据。 相似文献
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野生牧草金荞麦与贵州省推广牧草栽培效益比较试验初报 总被引：4，自引：0，他引：4
金荞麦Poyigonum cymosum是一种药饲兼用的贵州野生植物资源,通过金荞麦与贵州省目前主要推广牧草的田间试验比较,研究金荞麦和推广牧草资源的产量、品质和饲用的不同特点。试验初步认为:金荞麦是一种高产优质的牧草资源,其产量与菊苣Cichorium intybus相似,比一般推广牧草如苜蓿Medicagosativa等高出2~3倍。其粗蛋白含量与菊苣相似,接近苜蓿。金荞麦的适口性好,耐瘠抗劣性强,易繁殖成活,有较大的推广潜力。 相似文献
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It is often difficult to control parasite loads in captive animals in zoological institutions and there is the concern of rising drug resistance. Therefore, alternatives to parasite control should be investigated. Neem fruit (Azadirachta indica) is a herb native to India and has long been used in ancient Ayurvedic medicine. It has also been noted to have anti-parasitic properties and has been used as an alternative method of combatting endoparasitism in various animals successfully. In this randomized controlled trial, the effectiveness of neem oil as a dewormer was compared to the chemical dewormer wormout gel (Vetafarm, 20 g/L Praziquantel, 20 g/L Oxfendazole) and was tested in a group of 18 eared doves (Geopelia striata). These 18 doves were randomized equally into 3 groups where one group was given neem oil daily, the second group was given wormout gel (Vetafarm, 20 g/L Praziquantel, 20 g/L Oxfendazole) once and the last group was the control group and given water daily. Faecal tests (wet mount and McMaster egg counting technique) were done by a veterinary lab technician that was blinded to the group assignment. The faecal tests were done and the weight of each bird was recorded on admission, daily during the treatment period and 1 day after the treatment was finished. The daily weights and faecal test results were recorded down and the mean parasite ova per gram of feces and mean number of days where the parasite ova count was negative were calculated for each dove. The means were analyzed and the results showed that there were no significant benefits of using neem oil as a dewormer as compared to the wormout gel. Even though there was no significant benefits of using neem oil as a dewormer as compared to wormout gel in this study, further studies should be performed on neem as a natural antiparasitic alternative as not much knowledge about neem use in birds is available. 相似文献
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Zygomatic mucocoele is reported as a postoperative complication occurring secondary to a caudal hemimaxillectomy in a two-year-old Labrador retriever. The dog was presented with a history of a rapidly growing oral mass, identified as a soft tissue sarcoma. A caudal hemimaxillectomy via an intraoral approach was performed as treatment for local control of the oral mass. Fifteen days postoperatively, periorbital swelling and exophthalmos developed on the ipsilateral side. The degree of swelling progressed and was identified by computed tomography, ultrasound and cytology as a salivary mucocoele. Zygomatic sialoadenectomy was performed via a modified lateral approach with zygomatic osteotomy. A small amount of discharge persisted from the surgical site but gradually resolved. Recurrence of the periorbital swelling and exophthalmos was noted 25 days later and further surgery was performed to excise residual salivary tissue. Adjuvant radiotherapy was performed, however local recurrence of the oral mass was identified 5 months postoperatively and the patient subsequently euthanased. Salivary mucocoele has been cited as a possible postoperative complication following maxillectomy and mandibulectomy procedures; however to the authors’ knowledge, only one previous case report exists in the literature. The current case documents a zygomatic salivary mucocoele occurring subsequent to caudal hemimaxillectomy. 相似文献
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Interleukin-8, a member of the chemokine family of cytokines, is a potent neutrophil chemoattractant in many non-rodent species. In this study, recombinant bovine interleukin-8 (rbIL-8) was expressed in bacteria as a glutathione-S-transferase fusion protein. The fusion protein was purified by glutathione-Sepharose affinity chromatography and recombinant rbIL-8 was eluted by cleaving with thrombin. The purified rbIL-8 molecule was approximately 8 kDa and was confirmed as authentic IL-8 by Western analysis. Recombinant bovine IL-8 induced specific dose-dependent in vitro chemotaxis of neutrophils at doses as low as 1.0 ng/ml, and this activity was inhibited by pre-treatment of rbIL-8 with a monoclonal antibody to ovine IL-8. Neutrophils exposed to rbIL-8 developed pseudopodia and became elongated as determined by microscopic analysis and flow cytometry. Injection of 3.3 ng to 3.3 microg of rbIL-8 into the skin of a normal calf induced dose-dependent recruitment of neutrophils but not eosinophils. Intravascular margination of neutrophils was obvious at the injection sites from 15 to 60 min after administration of rbIL-8, and extravascular neutrophil numbers increased steadily from 1 to 18 h after injection. Neutrophils with morphologic features of apoptosis were detected in these lesions at 18 and 30 h after injection, and this correlated with reduction in the number of dermal neutrophils. These results confirm unequivocally that bovine IL-8 functions as a neutrophil, but not an eosinophil, chemoattractant in vitro and in vivo. 相似文献
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Williams JH Van Wilpe E Momberg M 《Journal of the South African Veterinary Association》2005,76(2):90-98
A 14-year-old lioness, originating from Etosha in Namibia, and a member of a pride in Pilanesberg National Park since translocation in 1994, was euthanased due to fight-related vertebral fracture and spinal injury, incurred approximately 6-8 weeks previously. Blood specimens collected at the time of death showed mild anaemia and a leukogram reflecting stress and chronic infection. Necropsy conducted within 2 hours of death was on a dehydrated, emaciated animal with hindquarter wasting and chronic traumatic friction injuries from dragging her hindlegs. There was cellulitis in the region of bite-wounds adjacent to the thoraco-lumbar vertebral fracture, at which site there was spinal cord compression, and there was marked intestinal helminthiasis. The outer renal medullae appeared pale and waxy and the liver was macroscopically unremarkable. Histopathology and electron microscopy of the kidneys revealed multifocal to coalescing deposits of proximal medullary interstitial amyloid, which fluoresced strongly with thioflavine T, and was sensitive to potassium permanganate treatment prior to Congo Red staining, thus indicating inflammatory (AA) origin. There was diffuse hepatocyte dissociation, as well as numerous binucleated and scattered multinucleated (up to 8 nuclei/cell) hepatocytes, with swollen hepatocyte mitochondria, in liver examined light microscopically. Ultrastructurally, the mono-, bi- and multinucleated hepatocytes contained multifocal irregular membrane-bound accumulations of finely-granular, amorphous material both intra-cytoplasmically and intra-nuclearly, as well as evidence of irreversible mitochondrial injury. The incidence and relevance in cats and other species of amyloidosis, particularly with renal medullary distribution, as well as of hepatocyte dissociation and multinucleation, as reported in selected literature, is briefly overviewed and their occurrence in this lioness is discussed. 相似文献
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Chisato Hayakawa Masayuki Kimura Yusuke Kuroda Seigo Hayashi Kazuya Takeuchi Satoshi Furukawa 《Journal of toxicologic pathology》2021,34(3):251
It is extremely rare to have multiple spontaneous proliferative lesions in young adult rats. Here, we report the occurrence of different proliferative lesions in multiple tissues of a 7-week-old female rat in a 1-week repeated toxicity study. Grossly, multiple white patches and nodules in the bilateral kidneys, femoral and subcutaneous masses, and a nodule in the liver were observed. Renal lesions were diagnosed as renal mesenchymal tumors. One of the femoral subcutaneous masses was diagnosed as an adenolipoma consisting of mammary epithelial cells and mature adipocytes. The other femoral and abdominal subcutaneous masses were diagnosed as lipomas consisting of mature adipocytes. The liver nodule was diagnosed as non-regenerative hepatocellular hyperplasia, which was characterized by the proliferation of slightly hypertrophic hepatocytes. In the cauda equina, the growth of enlarged Schwann cells around the axon was observed, and this lesion was diagnosed as a neuroma. 相似文献
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J C Seely S F Cosenza C A Montgomery 《Journal of the American Veterinary Medical Association》1978,172(12):1427-1429
An enlarged nodular mass, assumed to be the prostate gland because of location and consistency, was palpated in the pelvic region of a 10-year-old German Shepherd Dog. Castration was performed as a therapeutic procedure after an estrogenic hormone failed to reduce the size of the mass. A tumor identified histologically as a seminoma was in the right testicle. The dog died 1 month after castration, and generalized metastatic neoplasia was observed at necropsy. The mass that involved the neck of the bladder and the metastases were identified as leiomyosarcoma. 相似文献
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Duncan McNab McEachran's early history and involvement in the formation of the Montreal Veterinary College as well as in livestock inspection were reviewed. His contribution to the beginning of the ranching industry in western Canada was explored in detail. In 1881, McEachran helped to establish the Cochrane Ranche, which was the first great ranch to be started in southern Alberta. He was employed as the ranch's resident general manager until 1883, in which year the Waldrond Ranch was established. McEachran was this ranch's president and general manager until approximately 1909. During this time, he came under considerable criticism from both The MacLeod Gazette and The Calgary Herald. As Dr. McEachran maintained his obligations to the ranches while he was directing the veterinary college in Montreal as well as chief inspector of livestock for Canada, it was concluded that this feat alone would rank him as a remarkable historical figure. 相似文献
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2019年1月,A县畜牧服务中心依法取缔一处生猪屠宰窝点。经立案调查,根据《动物防疫法》《生猪屠宰管理条例》等法律法规相关规定,对涉嫌未经定点从事生猪屠宰活动的当事人郭×给予没收5头生猪及猪肉产品,并处货值4倍罚款的行政处罚。郭×对处罚不服而提起行政诉讼,法院判决撤销行政处罚行为,畜牧中心重新作出处罚决定后,案件结案。本文对案件查办中的主体适格、货值计算、价格认定、逃避检疫责任追究、自宰自食认定等相关环节进行了分析,以期与广大同行探讨。 相似文献