共查询到20条相似文献，搜索用时 31 毫秒
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Moore MJ 《Science (New York, N.Y.)》2005,309(5740):1514-1518
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MicroRNA-directed cleavage of HOXB8 mRNA 总被引：1，自引：0，他引：1
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Pillai RS Bhattacharyya SN Artus CG Zoller T Cougot N Basyuk E Bertrand E Filipowicz W 《Science (New York, N.Y.)》2005,309(5740):1573-1576
MicroRNAs (miRNAs) are approximately 21-nucleotide-long RNA molecules regulating gene expression in multicellular eukaryotes. In metazoa, miRNAs act by imperfectly base-pairing with the 3' untranslated region of target messenger RNAs (mRNAs) and repressing protein accumulation by an unknown mechanism. We demonstrate that endogenous let-7 microribonucleoproteins (miRNPs) or the tethering of Argonaute (Ago) proteins to reporter mRNAs in human cells inhibit translation initiation. M(7)G-cap-independent translation is not subject to repression, suggesting that miRNPs interfere with recognition of the cap. Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression. 相似文献
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MicroRNA expression in zebrafish embryonic development 总被引：5，自引：0，他引：5
Wienholds E Kloosterman WP Miska E Alvarez-Saavedra E Berezikov E de Bruijn E Horvitz HR Kauppinen S Plasterk RH 《Science (New York, N.Y.)》2005,309(5732):310-311
MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid-modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity. 相似文献
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MicroRNAs modulate hematopoietic lineage differentiation 总被引：8，自引：0，他引：8
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Down-regulation of LFA-1 adhesion receptors by C-myc oncogene in human B lymphoblastoid cells 总被引：16，自引：0，他引：16
G Inghirami F Grignani L Sternas L Lombardi D M Knowles R Dalla-Favera 《Science (New York, N.Y.)》1990,250(4981):682-686
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RNA: guiding gene silencing 总被引：1，自引：0，他引：1
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Giraldez AJ Mishima Y Rihel J Grocock RJ Van Dongen S Inoue K Enright AJ Schier AF 《Science (New York, N.Y.)》2006,312(5770):75-79
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新疆主要棉区棉花黄萎病菌致病力分化及其遗传多样性分析 总被引：2，自引：1，他引：1
测定了源于新疆棉区的22个棉花黄萎病菌单孢菌株和3个落叶型棉黄萎病菌菌株T9、VD8和V151对4个棉花品种上的致病力.采用随机引物聚合酶链反应(RAPD)技术分析了这些菌株的遗传多样性.结果表明:供试的25个棉花黄萎病菌之间的致病力存在显著差异(P<0.05).在各棉花品种上,22个新疆菌株中都有与T9、VD8或V151的致病力差异不显著的菌株,说明新疆存在较强致病力的棉花黄萎病菌菌系.RAPD分析表明,供试的8个随机引物中6个可以从25个菌株的基因组DNA中稳定地扩增出多态性DNA片段.对扩增片段统计结果表明,供试菌株间遗传相似系数变化幅度为0.57～1.00.聚类分析表明,在阈值0.625处可将25个菌株分为4个RAPD群(命名为RG1、RG2、RG3和RG4),RG1包括2个菌株,即B、BL-19菌株; RG2 包括5个菌株,即BL-17、BL-13、N-5、H、N-16;RG3为最大一个亚群,包括T9、SL等16个菌株;RG4包括2个菌株,即VD8和V151.综合分析表明新疆棉花黄萎病菌的致病力强弱和菌株的采集地等遗传背景存在一定的差异,这可能与各主产棉区的频繁引种有着直接的联系. 相似文献
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Hall N Karras M Raine JD Carlton JM Kooij TW Berriman M Florens L Janssen CS Pain A Christophides GK James K Rutherford K Harris B Harris D Churcher C Quail MA Ormond D Doggett J Trueman HE Mendoza J Bidwell SL Rajandream MA Carucci DJ Yates JR Kafatos FC Janse CJ Barrell B Turner CM Waters AP Sinden RE 《Science (New York, N.Y.)》2005,307(5706):82-86