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1.
In order to identify possible peripheral markers of illegal treatments with growth-promoting agents in veal calves, beta-adrenergic receptor (beta-AR) and glucocorticoid receptor (GR) concentrations were measured in lymphocytes of 12 male Friesian crossbred calves (six controls and six treated). The animals received a cocktail of anabolic and re-partitioning agents [17beta-oestradiol: 3 x 10 mg intramuscular (i.m.) doses at 17-day intervals; dexamethasone sodium phosphate: 4 mg/day for 6 days and 5 mg/day for six further days dissolved in milk; and clenbuterol: 20 microg/kg/day dissolved in milk for the last 40 days before slaughter]. Blood samples were collected by venipuncture at different time points and lymphocytes were isolated by density gradient centrifugation. Lymphocyte beta-AR and GR levels were measured by binding assays. Treatment with re-partitioning agents caused a significant down-regulation of lymphocyte beta-ARs 19 days after the beginning of clenbuterol administration and at day 55 (after dexamethasone withdrawal, just before slaughter). This phenomenon was partially reversed at day 50, after dexamethasone administration, at which time a significant decrease in GR concentrations also occurred. For both types of receptors, no significant changes in the dissociation constant values were observed at any time point. Lymphocytes express measurable concentrations of beta-ARs and GRs and the measurement of receptor levels highlights the fluctuation of receptor expression due to the dynamic interaction of the drugs used in combination. Lymphocyte receptor determination could therefore be included in a battery of biological assays to detect illegal treatments with anabolic agents in veal calves in the light of a multivariate approach.  相似文献   

2.
Biochemical modifications induced by a combination of anabolic compounds in target organs of male veal calves have been evaluated. Six male Friesian crossbred calves were treated with of 17beta-estradiol, dexamethasone sodium phosphate and clenbuterol or served as controls. beta-Adrenoceptors (beta-ARs) were measured in myocardium, lung, spleen, cerebral cortex, hippocampus, thalamus, hypothalamus, and hypophysis, glucocorticoid receptors (GRs) in the spleen and androgen receptors (AnRs) in the testis, by binding assay. A significant decrease in beta-ARs was observed in all tissue samples from treated animals. In the spleen the two GR subtypes found, low (LA) and high (HA) affinity GRs, were down-regulated by the treatment. A significant (P<0.05) decrease of testis weight and a significant (P<0.05) up-regulation of AnRs was also observed. Our data demonstrate that long-term treatment with anabolic compounds markedly affects receptor concentrations in target organs of male veal calves. Thus, studies investigating biological assays as screening methods to detect such compounds should be encouraged.  相似文献   

3.
The effect of transportation on peripheral blood lymphocyte subsets in 24 calves was investigated by flow cytometry. Blood was collected before departure, on arrival, at 24h and 1 week after arrival. Highest leucocyte and neutrophil counts, associated with increased concentrations of cortisol and catecholamines, indicated that stress was maximal upon arrival. At this time, a decrease in the percentages of all T lymphocyte subsets was evident, while they did not decrease as absolute counts. The proportion of CD21(+) cells did not change, indicating that the relative reduction of T lymphocyte subsets was not related to an increase in B lymphocytes. These variations may be due to the increase of a natural killer (NK) cell subset. NK cell expansion, together with increasing lymphocyte count and increasing major histocompatibility complex class II expression, may indicate stress-induced stimulation of the immune system.  相似文献   

4.
The experiment was designed to evaluate the effects of housing system and short-term transportation on the pituitary and adrenal response and on blood progesterone concentrations of beef cattle. Since the use of steroid hormones in farm animals has been banned in the EU (Council Directive 96/22/EC), it seems important to study the possible modifications in serum progesterone concentrations induced by stress in cattle. Thirty-two, 6 months old male Piedmontese beef cattle (16 reared in a littered loose house, Group A, and 16 housed in a littered tying stall barn, Group B) were blood sampled at T1 (6 months old), T2 (12 months old), T3 (18 months old, before transportation to the slaughterhouse) and T4 (after transportation to the slaughterhouse) in order to measure hormonal concentrations and lymphocyte glucocorticoid (GR) and β-adrenergic (β-AR) receptor concentrations. Circulating hormone concentrations were measured using commercial radioimmunoassay kits, whereas lymphocyte receptor density was determined through binding assays. In beef cattle housed in tie stall barn a significant increase in serum cortisol concentration was observed at T3, whereas there was no effect of the housing system on blood progesterone concentrations. Short-term transportation caused a significant increase in blood cortisol and catecholamine concentrations in both groups, whereas lymphocyte GR and β-AR significantly decreased in Group A. Our data confirm the activation of the hypothalamic-pituitary-adrenal axis and the catecholaminergic system in short-term transportation and suggest that the stress-induced increase in circulating progesterone concentrations does not exceed the limit established by pending legislation.  相似文献   

5.
The objective of this study was to determine the effect of two transport journeys (3 and 16 h) and four lairage times at the abattoir (3, 6, 12 and 24 h) on some stress indicators in steers to validate the current Chilean legislation of a minimum lairage period of 12 h for cattle arriving at abattoirs.The study was carried out twice, 80 steers in the winter and 80 in the summer of 1999. Forty steers were transported for 3 h and 40 for 16 h. At arrival in the slaughterhouse, the 40 transported steers were randomly divided in four lairage groups. Blood samples were collected before loading at arrival at the slaughterhouse and at slaughter during exsanguination.Mixed models were used to determine the associations between the stress indicators and the independent variables while correcting for the repeated observations on animals. Lairage increased plasma cortisol and PCV values independent of the transport duration. Glucose concentrations in steers transported for 16 h further increased after 3 and 6 h of lairage but decreased after 12 and 24 h of lairage. In steers transported for 3 h, glucose concentrations only increased after 24 h of lairage. After 24 h of lairage, the steers transported for 16 h had increased βHB concentrations compared with those transported for 3 h. Plasma CK activity was increased in steers after transport but was not affected by lairage. The study shows that there is no beneficial effect on the welfare of the animals by a long lairage time at the abattoir.  相似文献   

6.
Comparisons were made of the behaviour, physiology and meat quality of 24-week-old male veal calves (mean live weight 234 kg) that were slaughtered either at the veal unit (the control group) or following 3 h transportation at space allowances of either 0.7 or 0.95 m2 calf−1 and 1.5 h of lairage. Compared with nontransported calves, transported calves had a greater heart rate during the journey and during lairage; greater plasma cortisol concentration immediately after the journey, but not after 1.5 h of lairage; and greater plasma creatine kinase activity immediately after the journey and after 1.5 h of lairage. The transported calves did not lie down during the journey. There was no effect of space allowance on either the heart rate or frequency of potentially traumatic events during the journey or the plasma cortisol concentration and plasma creatine kinase activity sampled after the journey. There was no effect of transport on either carcase bruising or muscle pH 24 h after slaughter. Although compared with ‘on-farm’ slaughter, the transport and handling associated with the journey to the slaughterhouse were stressful to the calves; there were no adverse effects of transport on either carcase bruising or meat quality.  相似文献   

7.
This study examined the effects of road transportation on metabolic and immunological responses in dairy heifers. Twenty Holstein heifers in early pregnancy were divided into non‐transported (NT; n = 7) and transported (T; n = 13) groups. Blood was collected before transportation (BT), immediately after transportation for 100 km (T1) and 200 km (T2), and 24 h after transportation (AT). The T heifers had higher (P < 0.05) blood cortisol and non‐esterified fatty acid concentrations after T1 and T2 than did NT heifers. By contrast, the T heifers had lower (P < 0.05) serum triglyceride concentrations after T1 and T2 than had the NT heifers. The serum cortisol and triglyceride concentrations returned (P > 0.05) to the BT concentrations at 24 h AT in the T heifers. The granulocyte‐to‐lymphocyte ratio and the percentage of monocytes were higher (P < 0.05) after T2 in the T heifers than in the NT heifers, suggesting that transportation stress increased the numbers of innate immune cells. T heifers had higher (P < 0.01) plasma haptoglobin concentrations than NT heifers 24 h AT. In conclusion, transportation increased cortisol secretion and was correlated with increased metabolic responses and up‐regulation of peripheral innate immune cells in dairy heifers.  相似文献   

8.
Plasma sodium concentrations, packed cell volume, total plasma protein concentration and dermal skinfold thickness were used to assess the state of hydration of 113 transported calves before and after lairage and in 30 control calves on the farm of origin. Skin thickness increased significantly (P less than 0.05) and total protein increased slightly during transport and decreased during lairage. These changes suggest that transport may cause dehydration and lairage may help in recovery. Plasma potassium concentration decreased during transport, but the effect was inversely related to the distance travelled, and the concentration increased during lairage. These changes are consistent with recovery from initially high cortisol levels at loading. The resting behaviour of 150 transported calves was recorded hourly for six hours. More time was spent resting and sleeping by the transported calves than has been reported for non-transported calves and more still by small transported calves, suggesting that transport is exhausting, that lairage helps recovery and that small calves are more adversely affected. During lairage the numbers of calves asleep decreased to values reported for normal calves, suggesting that 10 hours lairage was adequate. However, small calves did not return to their normal rest patterns within the observation period.  相似文献   

9.
The objective of this study was to examine the effect of abrupt weaning (inclusive of social group disruption and maternal separation) on the physiological mediators of stress and measures of immune function. Thirty-six male and 36 female calves (Limousin and Charolais crosses), habituated to handling, were blocked by sex, weight, and breed of dam and randomly assigned, within block, to either a control or abruptly weaned group. Animals were separated into the respective treatment groups at 0 h. Calves were bled at -168, 6 (males only), 24, 48, and 168 h after weaning, and the behavioral reaction of calves to handling was scored. Cortisol, catecholamine (not sampled at -168 h), acute-phase protein concentrations, and in vitro interferon-gamma production and neutrophil:lymphocyte ratio were measured. The effects of weaning, calf sex, time, and their respective interactions were described. Disruption of the established social group at 0 h increased (P < 0.001) the plasma cortisol concentration and neutrophil:lymphocyte ratio and decreased the leukocyte concentration (P < 0.001) and the in vitro interferon-gamma response to the mitogen concanavalin-A (P < 0.001) and keyhole limpet hemocyanin (P < 0.001) for weaned and control animals compared with -168 h. There was no effect of weaning or sex on the behavioral reaction of calves to handling. Plasma cortisol and adrenaline concentrations were not affected by weaning or sex. Plasma noradrenaline concentration was influenced by weaning x sex (P < 0.05) and time x sex (P < 0.05). The response increased for male calves with weaning and increased with each sampling time after weaning. For heifers, the response was not affected by weaning and plasma concentrations decreased at 168 h after weaning. There was no effect of weaning or sex on leukocyte concentration. The neutrophil:lymphocyte ratio increased after weaning (P < 0.01) and was affected by sex (P < 0.05). Weaning decreased (P < 0.05) the in vitro interferon-gamma response to the antigen keyhole limpet hemocyanin. There was a time x weaning x sex (P < 0.05) interaction for fibrinogen concentration but no effect of treatment on haptoglobin concentration. Abrupt weaning increased plasma cortisol and noradrenaline concentrations that were accompanied by attenuation of in vitro interferon-gamma production to novel mitogen and antigen complexes up to 7 d after weaning.  相似文献   

10.
The objective herein was to estimate heterosis and breed effects in purebred and crossbred Romosinuano, Brahman, and Angus calves on acute phase protein response to weaning and transportation. Calves (n = 1,032) were weaned in September of 2002, 2003, and 2004 at approximately 7 mo of age. Approximately 28 d after weaning, steer calves (n = 482) were transported 1,800 km (20 h) to Oklahoma. Concentrations of 3 acute phase proteins (ceruloplasmin, fibrinogen, and haptoglobin) were measured in blood samples. Calves (steers and heifers) were sampled at weaning, and 24 and 72 h postweaning. For separate analyses, steers sent to Oklahoma were sampled before shipment, upon arrival, and 24 and 72 h after arrival. Combinations of the following fixed effects were investigated: sire breed, dam breed, sampling time, birth location, calf sex (weaning only), year, cow age, and interactions. Effects of special interest were sire breed x dam breed as an indication of breed group of calf, and the interaction of sire and dam breeds with sampling time. Weaning age and BW were investigated as linear and quadratic covariates. Sire of calf within sire breed was a random term. The correlation structure of repeated measures was determined by comparison of information criterion values for different structures within each analysis. In general, plasma acute phase protein concentrations in weaned calves increased with sampling time. Concentrations in the transported steers increased through sampling at 24 h after arrival, and were lower at 72 h. Significant estimates of heterosis were detected for Brahman-Angus haptoglobin concentrations at weaning (0.38 +/- 0.14 mg/dL x 100; 44%), and for Romosinuano-Angus fibrinogen concentrations at weaning (11.4 +/- 5.5 mg/dL; 10%) and in transported steers (22.5 +/- 8.4 mg/dL; 20%). The direct effect of Romosinuano was to increase (P <0.004) ceruloplasmin concentrations of weaned calves (4.1 +/- 0.9 mg/dL) and of transported steers (3.9 +/- 1.3 mg/dL). The direct effect of Angus was to lower ceruloplasmin concentrations in weaned calves (-3.9 +/- 1.2; P = 0.001). Significant maternal effects were detected at weaning for ceruloplasmin concentrations in Romosinuano (-1.4 +/- 0.5 mg/ dL) and Angus (1.6 +/- 0.7 mg/dL) and fibrinogen concentrations in Brahman calves (-17.7 +/- 8.8 mg/dL). These data imply that acute phase protein concentrations in response to weaning and transportation are impacted by cattle breed.  相似文献   

11.
Effects of stress on concentrations of cortisol and corticosteroid-binding globulin (CBG) in blood plasma and on glucocorticoid receptor concentrations in muscle cytosol were studied in pigs representing three Halothane (Hal) genotypes (NN, Nn, nn). At 12 wk of age, animals were divided into four groups: pigs subjected to transport (5 h in a truck), pigs treated with amperozide prior to transport, pigs not transported but treated with amperozide and pigs neither transported nor given amperozide. Animals were slaughtered the week they reached 100 kg live weight (3 mo later). The Hal gene showed no major influence on the variables studied except for cortisol concentrations (P = .06) measured directly after transport at 12 wk of age (NN = 66.8 nM, Nn = 61.4 nM, nn = 69.4 nM). However, the response in each Hal genotype differed, depending on whether or not the pigs had been exposed to transport. Two weeks after transport, NN pigs developed higher cortisol concentrations than untransported animals, whereas the response was reversed in nn animals; Nn pigs showed no difference in this regard. At slaughter, the effect of transport (12 wk of age) on cortisol and CBG was still apparent. In NN pigs cortisol and CBG concentrations were elevated (P less than or equal to .05, P = .08, respectively), whereas concentrations tended to be lower in nn pigs (P = .17, P = .07, respectively) when compared with untransported pigs. Transported pigs had lower receptor concentrations at slaughter (P less than or equal to .01) than untransported pigs. However, pigs given amperozide in connection with transport had a receptor concentration comparable to that in untransported pigs. Our study shows conclusively that transport stress had long-term effects on cortisol, CBG and glucocorticoid receptor concentrations. In addition, amperozide had long-term effects on cortisol and receptor concentrations.  相似文献   

12.
Thirty-eight mature horses were assigned to one of two equal groups to evaluate two treatments consisting of either 24 hours of continuous road transport (24T) or two 12-hour periods of transport separated by off-loading, resting and feeding the horses for 12 hours (12/12T). A subset of six horses from each group served as controls for the other group. The horses were loaded into a commercial straight-deck trailer and travelled loose in one of two standard-sized compartments. After the journeys the horses were put back into their paddocks for a 24-hour recovery period. Venous blood samples were collected before loading, after unloading and after the 24-hour recovery period. Transport significantly increased the horses' cortisol concentrations, neutrophil counts and neutrophil:lymphocyte (nl) ratios, and decreased the numbers of all the lymphocyte subpopulation cell types. Collectively, no significant differences were observed between the two treatments in the horses' cortisol concentrations, total leucocyte counts, neutrophil and lymphocyte counts, nl ratios, and the cd8a+ and cd21+ lymphocyte subpopulations, but there were differences in the numbers of cd3+, cd4+, and cd8b+ subpopulations. The inclusion of a 12-hour rest-stop interrupted the transport-related decline in the lymphocyte subpopulations and allowed them to recover towards their resting levels.  相似文献   

13.
Objectives of these studies were to determine the effects of flunixin meglumine (FM) administration on early embryonic mortality and circulating PG and cortisol concentrations in transported and non-transported cows. Cows (n = 483) from 3 locations were used to evaluate the effects of transportation and FM approximately 14 d after AI on the establishment of pregnancy and serum concentrations of progesterone, PGF metabolite (PGFM), and cortisol. Treatments were transport (n = 129), transport + FM (n = 128), no transport (n = 130), and no transport + FM (n = 96). Multiparous cows (n = 224) were used at 2 locations, and nulliparous cows (n = 259) were used at 1 location. The no transport + FM treatment was used at only 2 locations. Flunixin meglumine (approximately 1.1 mg/kg of BW; i.m.) was administered before the cows were separated into transportation groups. Transportation included 4 to 6 h of transportation, without calves, via semitractor trailer. Nontransported cows remained penned, with their calves in adjacent pens, during the same period as the transported cows. Blood samples were collected from all cows before and after treatment and, at 2 locations, approximately 3 h after the onset of treatment. Location affected AI pregnancy rate (P < 0.01). Treatment effects, although not significant (P = 0.16), were of a magnitude to be considered practically important. Cows that received transportation + FM tended (P = 0.07) to have greater AI pregnancy rates (74%) than those that did not receive FM (66%), irrespective of transportation. Cortisol concentration was greater (P < 0.05) for transported cows than for nontransported cows. Cows receiving FM had greater (P < 0.05) AI pregnancy rates than non-FM cows (71 vs. 61%, respectively). Cows receiving transportation had lower (P < 0.01) mean PGFM concentrations than nontransported cows (45.4 vs. 54.6 pg/mL, respectively), and cows receiving FM had lower (P < 0.01) mean PGFM concentrations than non-FM cows (39.4 vs. 60.6, respectively). We conclude that transportation of cows approximately 14 d after AI increased serum cortisol concentrations but did not affect AI pregnancy rates. However, treatment of cows with FM increased AI pregnancy rates, irrespective of whether they were transported.  相似文献   

14.
OBJECTIVE: To compare efficacy of flunixin meglumine versus carprofen in controlling pain under field conditions following castration by use of an external clamping technique in calves that received epidural anesthesia. ANIMALS: 40 male 5- to 6-month-old calves. PROCEDURES: Calves were allocated to 4 groups: castrated only (control calves; n=8); castrated 5 minutes after epidural injection of 2% lidocaine (epidural-alone treated calves; 8), castrated after epidural anesthesia and s.c. administration of flunixin meglumine (epidural-flunixin treated calves; 12), and castrated after epidural anesthesia and s.c. administration of carprofen (epidural-carprofen-treated calves; 11 [1 calf not included]). Plasma cortisol concentration was measured before and 6, 24, and 48 hours after castration. Time of arrival at the feed trough at 24 and 48 hours was observed. Calves were observed at 24 and 48 hours for 4 pain-related behaviors. RESULTS: At 6 hours, control calves had significantly higher plasma cortisol concentrations, compared with baseline values and those of epidural-flunixin- and epidural-carprofen-treated calves. At 24 hours, epidural-carprofen-treated calves had significantly lower plasma cortisol concentrations, compared with control calves. At 48 hours, epidural-carprofen-treated calves had plasma cortisol concentrations that were similar to baseline values and significantly lower than epidural-flunixin- and epidural-alone-treated calves. At 24 and 48 hours, epidural-carprofen-treated calves were first to arrive at the feed trough and had fewer pain-related behaviors. CONCLUSIONS AND CLINICAL RELEVANCE: s.c. administration of carprofen in combination with epidural injection of lidocaine may improve the welfare of calves castrated by use of an external clamping technique for up to 48 hours.  相似文献   

15.
Eight healthy Holstein-Friesian calves and 8 Massese lambs of either sex (10-15-days old) were used to evaluate the pharmacokinetics of thiamphenicol after intravenous (i.v.) and oral (p.o.) administration (30 mg/kg). Plasma concentrations of thiamphenicol were determined by high-performance liquid chromatography on blood samples collected over 24h following treatment. Pharmacokinetic variables of the drug were calculated for both species and after both administration routes. After intravenous administration of thiamphenicol, a rapid distribution phase was followed by a slower elimination phase and, when thiamphenicol was administered p.o., the bioavailability was about 60% in both species. The higher volume of distribution and the longer biological elimination half-lives in pre-ruminant compared with adult animals indicate that thiamphenicol distributes widely into the extravascular compartment of pre-ruminants. Interspecies differences were observed in the kinetic behaviour of thiamphenicol with respect to peak plasma concentration (C(max)), time of peak plasma concentration (T(max)), elimination half-life (T(1/2)) and total clearance (Cl(B)). In conclusion intravenous or oral administration of 30 mg/kg of thiamphenicol provides plasma concentrations higher than minimum effective concentrations inhibiting bacterial growth (MICs) against most pathogens in pre-ruminant lambs and calves.  相似文献   

16.
Brahman calves (n = 28) were used to evaluate the effect of environmental temperature during the 1st or 2nd d after birth. Calves were removed from their dams within 30 min of birth (newborn; D0) before suckling or at 20 h of age and fasted for 4 h before treatment (day-old; D1). Calves were placed in either a warm (W; 25 degrees C) or a cold (C; 5 degrees C) environment for 2 h and either maintained in or transferred to, respectively, W for 22 h. Blood samples were collected via jugular catheters at 15-min intervals beginning at initial placement in W or C through 3 h and at 4, 5, 6, 8, 10, 12, 14, 18, and 24 h. Rectal temperature (Tr) was recorded with each sample. Following the 60-min and 12-h samples, each calf was administered 1 liter of colostrum from its dam. Serum or plasma was analyzed for glucose, lactate, plasma urea nitrogen, triglycerides, nonesterified fatty acids, insulin, cortisol, triiodothyronine (T3), and thyroxine (T4). Rectal temperature of D0C calves was lower (P less than .05) than that of other calves from 75 min through 3 h. Insulin, lactate, T3, and plasma urea nitrogen concentrations were not different among all calves. Higher (P less than .01) cortisol and T4 concentrations were observed in D0 than in D1 calves. Cortisol (P less than .008) and nonesterified fatty acid (P less than .05) levels were greater in C than in W calves. All D0 calves had lower (P less than .0001) glucose concentrations than D1 calves until the 12-h feeding.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

17.
The objective of this study was to investigate the effect of transportation and commingling on measures of the acute-phase protein response in newly weaned beef calves. Thirty-two (Exp. 1; average BW = 266 +/- 20.8 kg) and thirty-six (Exp. 2; average BW = 222 +/- 34.6 kg) Brahman-crossbred calves were randomly allotted to one of four treatments (2 x 2 factorial arrangement [transportation x commingling] in a completely randomized design). Body weight and jugular blood were collected at weaning, after shipment, and 1, 3, and 7 d after transport for Exp. 1, and at weaning and 1, 5, 9, 13, 17, and 21 d after transport for Exp. 2. Feed intake within pen was recorded daily for Exp. 2. Plasma fibrinogen, ceruloplasmin, haptoglobin, and cortisol concentrations were determined for all collection times. Additionally, serum amyloid-A and alpha-acid glycoprotein concentrations were determined in Exp. 1 and 2, respectively. In Exp. 2, commingled calves tended (P = 0.13) to have a higher DMI than noncommingled calves (5.3 and 4.8 kg/d, respectively). Transported calves lost more BW than nontransported calves from the time of weaning to d 1 (2.0 and 3.1% more BW loss for Exp. 1 and 2, respectively). With the exception of haptoglobin in Exp. 1, each of the acute-phase proteins measured in these studies increased over each sampling day. In Exp. 1, transported calves had higher (P < 0.05) mean serum amyloid-A concentrations than nontransported calves (48.9 vs. 33.4 microg/mL). There was a significant sampling day x transportation interaction (P < 0.01) for fibrinogen, ceruloplasmin, and haptoglobin in Exp. 1; transported calves had higher concentrations of fibrinogen following transport and on d 2 and 3, and ceruloplasmin on d 3. Haptoglobin concentrations were higher (P = 0.04) in nontransported calves on d 1 and 2 of Exp. 1. In Exp. 2, overall mean haptoglobin concentrations were higher in nontransported vs. transported calves. The results of these studies indicate that stressors associated with transportation affect the acute-phase protein response in newly weaned beef calves. More research is needed to determine whether these proteins might be valuable indicators of stress following the weaning process.  相似文献   

18.
The objective of this study was to determine the influence of transportation on the serum concentrations of the cardiac biomarkers troponin I (cTnI) and creatine kinase-myocardial band (CK-MB) and on cortisol and lactate in horses. For this purpose, 10 horses were transported for 300 km. Blood samples were collected 24 hours before transport (T0), just before transport (T1), during transport at 50 km (T2), 100 km (T3), 200 km (T4), and 300 km (T5). An additional blood sample (T6) was collected 24 hours after transport. The median resting basal cTnI values in the horses were at T0, 0.000 ± 0.007 ng/mL and at T1, 0.01 ± 0.007 ng/mL. The median resting basal CK-MB values in the horses were at T0, 0.19 ± 0.05 ng/mL and at T1, 0.16 ± 0.05 ng/mL. Statistical analyses showed no significant differences of cTnI and CK-MB among the measured values (T0–T6). On the other side, the cortisol and lactate concentrations increased significantly (P < .01) at T2, T3, T3, and T4 compared with the resting values at T0. At T6, cortisol and lactate concentrations had returned to pretransport values, with no statistically significant differences compared with pretransport concentrations. In conclusion, the 300-km transportation of the horses did not influence the levels of the cardiac biomarkers, cTnI and CK-MB. The serum concentrations of cortisol and lactate, on the other side, increased significantly. The possible influence of transportation for longer distances or under more stressful conditions (higher temperature or in horses not used for transportation) on cTnI and CK-MB concentrations needs to be further investigated.  相似文献   

19.
Blood samples were collected from unstressed cattle and from cattle undergoing handling stress, transport stress and slaughter. The blood was analysed for ACTH, cortisol, thyroxine stimulating hormone, tri-iodothyronine (T3) and catecholamine concentrations, and for haematocrit, total plasma protein, plasma lipid, lactate and glucose concentrations. Compared to control values handling significantly increased T3, cortisol, lipid and lactate concentrations. Compared to handling, transport stress was associated with increased catecholamines and lactate concentrations, a decreased cortisol concentration and similar concentrations of T3, lipid and glucose. Compared to transport, slaughter resulted in high catecholamines, lactate and glucose, and low T3, cortisol and lipid levels. It is concluded that the response to stress has two phases, a hypothalamic-adrenal cortex phase which is associated with perceived environmental stress such as noise, and a sympathetic-adrenal-medulla phase which is associated with neurogenic stress such as transport or specifically the massive sympathetic discharge caused by stunning. Combinations of stresses produce a mixed response.  相似文献   

20.
The study was conducted to evaluate the effects of different lairage time after 8 h road transport on some blood indicators of welfare and meat quality traits in sheep. A total of 84 Ujimqin male sheep (average body weight 27.5 kg, 6 months old) were randomly allotted to one of seven groups: one control group (untransported) and six lairage groups (8 h road transport with 0, 2, 6, 12, 24 and 48 h lairage times respectively). No significant lairage time effects were observed on weight loss. Sheep in 48 h group showed lower hot carcass weight, dressing percentage and higher pH24 h than that in other groups. The total haem pigment contents in sheep meat rose and were higher in 24 and 48 h groups than that in the control group. After transport, sheep in 0, 2, 24 and 48 h groups showed higher serum creatine kinase activities, cortisol and glucose concentrations than that in control group. Sheep in lairage groups had higher serum triiodothyronine and thyroxine levels compared with the control sheep. Sheep in 48 h group showed significant higher packed cell volume, total protein and blood urea nitrogen than that in other groups. Compared with the control group, the white blood cell counts were higher in 0 and 48 h groups. The neutrophil counts in 24 or 48 h groups were higher than that in the control group. The opposite was true for lymphocyte counts. A 6–12 h lairage is recommended in terms of the present transported pattern.  相似文献   

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