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1.
Abstract. We studied the effects of red deer grazing and fence-line pacing on soil losses of contaminants (suspended sediment, Escherichia coli , phosphorus) and nitrogen species (ammonia, nitrate) via overland flow and soil physical properties (macroporosity, bulk density, saturated hydraulic conductivity, K sat) soon after (1 day) and 6 weeks after grazing on a Pallic pastoral soil in southern New Zealand. Fence-line pacing decreased the soil volume occupied by water, macroporosity and K sat, while increasing suspended sediment (to 0.226 g 100 mL−1), total P (to 2.0 mg L−1), mainly as particulate P (up to 90% of total P), and E. coli (to 3.52 log10 c.f.u. 100 mL−1) concentrations in overland flow at 1 day after grazing compared with soils from the rest of the paddock (0.148 g 100 mL−1, 0.86 mg L−1 and 2.86 log10 c.f.u. 100 mL−1, respectively). Although concentrations in overland flow were less at 6 weeks after grazing than at 1 day after grazing, losses of P, especially in fence-line soils, were still above recommended limits for surface water quality. Compared to P, losses of N species would be unlikely to have a significant impact on downstream water quality. Management strategies should be directed towards minimizing the occurrence of fence-line pacing to prevent contaminant loss and maintain water and soil quality.  相似文献   

2.
Contamination of agricultural soil by fecal pathogenic bacteria poses a potential risk of infection to humans. For the biosafety control of field soil, soil solarization in an upland field was examined to determine the efficiency of solarization on the inactivation of Escherichia coli inoculated into soil as a model microorganism for human pathogenic bacteria. Soil solarization, carried out by sprinkling water and covering the soil surface with thin plastic sheets, greatly increased the soil temperature. The daily average temperature of the solarized soil was 4–10°C higher than that of the non-solarized soil and fluctuated between 31 and 38°C. The daily highest temperature reached more than 40°C for 8 days in total in the solarized soil during the second and third weeks of the experiment. Escherichia coli in the solarized soil became undetectable (< 0.08 c.f.u. g−1 dry soil) within 4 weeks as a result, whereas E. coli survived for more than 6 weeks in the non-solarized soil. Soil solarization, however, had little influence on the total direct count and total viable count of bacteria in the soil. These results indicate that soil solarization would be useful for the biosafety control of soil contaminated by human pathogens via immature compost or animal feces.  相似文献   

3.
pp. 865–874
Occurrence and survival of fecal-contamination indicator bacteria (coliform bacteria, Escherichia coli and Salmonella ) in various manure and compost samples collected from 23 composting facilities mostly in Kyushu were investigated by using selective media. Coliform bacteria were detected on desoxycholate agar from 11 (38%) of 29 product samples (15 cow dung manure, 4 poultry manure, 2 biosolid compost and 8 food waste compost) at a range of 102 to 106 cfu g1 dry matter. From positive samples, 21 isolates of possible coliform bacteria were purified. Among them, species of coliform bacteria ( E. coli , E. vulneria , Pantoea sp. and Buttiauxella agrestis ) were identified whereas isolates of Serratia marcescens , not coliform bacteria, were also obtained, suggesting that careful observation was necessary to avoid false positive counting due to the presence of a red colony of S. marcescens that resembled coliform bacteria. Isolates of E. coli were tested for slide aggregation with a set of antiserum against pathogenic E. coli serotypes and negative reaction was obtained for all the isolates tested. Direct detection of E. coli on Chromocult coliform agar and Salmonella on MLCB agar resulted in none and 2 (17%) of 12 samples tested, respectively. The fate of fecal-contamination indicator bacteria as above was followed during compost production on 7 cases at 6 compost facilities and 4 patterns were observed: fecal-contamination indicator bacteria 1) decreased and finally disappeared, 2) decreased once but re-growth was occurred on products, 3) decreased to some extent but remained in products, 4) was not detected throughout production. These results suggest that some fecal-contamination indicator bacteria may survive compost production and appropriate temperature control would be significant for hygiene control of manure and compost.  相似文献   

4.
Two field experiments commencing in winter (December) and spring (April) were conducted to determine the fate of nitrogen (N) in cattle slurry following application to grassland. In each experiment three methods of application were used: surface application, and injection ± the nitrification inhibitor, nitrapyrin. Slurry was applied at 80t ha−1, (≡248 kg total N ha−1 in the winter experiment, and 262 kg N ha−1 in the spring experiment). From slurry applied to the surface, total losses of N through NH3 volatilization, measured using a system of wind tunnels, were 77 and 53 kg N ha−1 respectively for the winter and spring experiments. Injection reduced the total NH3 volatilization loss to ∼2 kg N ha −1. Following surface application, loss by denitrification, measured using an adaptation of the acetylene-inhibition technique, was 30 and 5 kg N ha−1 for the two experiments. Larger denitrification losses were observed for the injected treatments; in the winter experiment the loss from the injected slurry without nitrapyrin was 53 kgN ha −1, and with nitrapyrin 23 kgN ha−1. Total denitrification losses for the corresponding injected treatments in the spring experiment were 18 and 14 kg N ha −1. Apparent recoveries of N in grass herbage in both experiments broadly reflected the differences between treatments in total gaseous loss.  相似文献   

5.
Abstract. Escherichia coli serotype O157 is a virulent human pathogen the global incidence of which has increased. It has been demonstrated that cattle are the primary reservoir of this pathogen. This has serious implications for the land-based disposal of organic wastes such as cattle manure, cattle slurry and abattoir waste. Further, it also has serious ramifications for the protection of surface and groundwater drinking supplies and public access to pasture land. However, while soil and vegetation can be expected to directly influence the survival of this pathogen, there is a paucity of information concerning the behaviour and survival of E. coli O157 in agricultural environments. It appears that E. coli O157 presently contaminates between 1 to 15% of UK cattle herds, depending on region, and that faecal excretion of the bacterium shows a distinct seasonality which also reflects the incidence of human infections. E. coli O157 can remain viable in soil for greater than 4 months and appears to be a highly resilient pathogen possessing the capability to adapt easily to environmental stresses. While most human cases of E. coli O157 related food poisoning have been associated with the consumption of contaminated meat and dairy products, there is also evidence that human infection has occurred through the ingestion of contaminated soil, fruit and vegetables and drinking water. In this review the potential threat to human health posed by the application of contaminated organic wastes to soil and possible strategies for reducing the amount of pathogen entering the food chain are highlighted.  相似文献   

6.
Abstract. The effect of eight years of applications of five rates (0, 134, 269, 538 and 1075 m3 ha−1 a−1) of pig slurry on the soil strength two years later were studied in a field experiment. Soil strength in the 0–150 mm depth was measured on five occasions in winter using a hand-held recording cone penetrometer. On one occasion the penetration resistance at some depths greater than 100 mm was significantly ( P < 0.001) decreased by adding more than 269 m3 of slurry ha−1 a−1. On three occasions different amounts of slurry caused significant differences in the rate of increase of penetration resistance with depth. Large applications of slurry may decrease penetration resistance because they increase organic matter, thereby increasing the water retention of the soil.  相似文献   

7.
Abstract. Experiments on the effects of stockpiling soil on an opencast coal mine in Derbyshire showed that there were significant changes in the microbial community. Numbers of aerobic bacteria in stored soils ranged from 0.5 to 12.8 ± 107 colony-forming-units (CFU)g-1 with the smallest values being in the deepest parts of the oldest stores, whereas an adjacent undisturbed soil contained 6.6 ± 107 CFU g-1. There was a greater effect on the numbers of fungal spores, which ranged from 0.1 to 6.7 ± 105 CFU g-1 soil, all less than the 10 ± 105 CFU g-1 recorded for the undisturbed control soil. The number of fungal spores in the deepest part of the older soil stores was only 1/100 of the number in the undisturbed soil. This was mirrored by the biomass values, as determined by adenosine triphosphate (ATP) assay. Values of ATP ranged from 0.38 to 13.13 nmol g-1 as compared to 5.8 nmol g-1 in the undisturbed soil. All three of these microbiological properties decreased in value with both age and depth of storage. Neither anaerobic nor spore-forming bacterial numbers were greatly affected by storage.
The pH values tended toward neutrality in the deeper parts of the soil stores, and there was less organic matter in the older stores.  相似文献   

8.
At Onne in South-east Nigeria, drainage water was collected from four monolith lysimeters and analysed for nitrate. The lysimeters contained an acid sandy loam. At the start of the first rainy season two lysimeters received urea labelled with 15NO3– and two received no nitrogen fertilizer; all four were uncropped in the first year.
The peak concentrations of 15NO3– and of unlabelled (soil) NO3– were found after 2.5 pore volumes of water had passed through the lysimeters. Using the same soil in the laboratory after fine sieving, the peak concentration of tritiated water was found at 1 pore volume whereas nitrate leaching was retarded. The pattern of nitrate leaching was well described by miscible and immiscible models which included an adsorption coefficient for nitrate. Over the 2 years 81.4% of the 15N added at the start of the first rainy season was recovered in the drainage water.  相似文献   

9.
Previous trials have revealed variable responses of sago palm ( Metroxylon sagu Rottb.) to fertilizer application, particularly nitrogen (N). In the present study, we quantified the fertilizer use efficiency (FUE) of sago palm for the first time using 15N-labeled fertilizer in pot and field experiments. The pot experiment was conducted in Japan using a 2:1 mixture of sand to Philippine soil. The field experiment was conducted in Leyte in the Philippines. Both experiments consisted of three replicates in each of three treatments: control, 15N urea at 50 kg N ha−1 and 15N urea at 100 kg N ha−1. The N uptake of sago palm increased significantly, but inconsistently with increasing N application. The few instances of a significant increase in N uptake did not translate into significant improvements in growth parameters, except for the number of leaflets in the pot experiment. The FUE values for sago seedlings (< 6 months) in the pot experiment treated with 50 and 100 kg N ha−1 were 10.5 and 13.2%, respectively, whereas for the 2-year-old sago palms in the field, the corresponding FUE values were 14.8 and 12.0%. The FUE values were similar at the two levels of N application in both experiments. Sago growth parameters appeared to be insensitive to N application, suggesting that the form of N and the timing of N fertilization are important factors for sago palms. Therefore, the use of N fertilizer in sago production can only be justified after determining and fully understanding the response of sago palm to N application.  相似文献   

10.
Peat land has been considered as an alternative type of land for agricultural development especially in the tropics. In the present study, the N-supplying capacity, one of the most important soil properties in terms of crop production, of peat soils was examined. Ten peat soil samples were collected from Indonesia, Malaysia, and Japan. Gross N mineralization in the soil samples was estimated using a zero-order model, and kinetic parameters of mineralization were determined using a simple type model. Soil organic matter composition was investigated using 13C CPMAS NMR. Mineralization potential ( N 0), apparent activation energy ( E a), and mineralization rate constant ( k ) ranged between 571–2,445 mg kg−1, 281–8,181 J mol−1, and 0.009–0.020 d−1, respectively. Although none of the parameters showed a significant correlation with the soil C/N ratio, a negative correlation was observed between the k value and the ratio of the proportion of alkyl C in total C to that of O -alkyl C estimated by 13C CPMAS NMR. The latter suggested that the k values were higher in the peat soils relatively rich in readily decomposable organic matter including carbohydrates.  相似文献   

11.
Abstract. Nitrate nitrogen (NO3-N) leaching from animal production systems in the northeast USA is a major non-point source of pollution in the Chesapeake Bay. We conducted a study to measure NO3-N leaching from dairy slurry applied to orchardgrass ( Dactylis glomerata L., cv. Pennlate) using large drainage lysimeters to measure the direct impact of four rates of slurry (urine and faeces) N application (0, 168, 336, 672 kg N ha−1 yr−1) on NO3-N leaching on three soil types. We then used experimentally-based relationships developed earlier between stocking density and NO3-N leaching loss and leachate NO3-N concentration to estimate the added impact of animal grazing. Nitrate N leaching losses from only dairy slurry applied at the 0, 158, 336, and 672 kg N ha−1 yr−1 rates were 5.85, 8.26, 8.83, and 12.1 kg N ha−1 yr−1, respectively with corresponding NO3-N concentrations of 1.60, 2.30, 2.46, and 3.48 mg l−1. These NO3-N concentrations met the 10 mg l−1 US EPA drinking water standard. However, when a scenario was constructed to include the effect of NO3-N leaching caused by animal grazing, the NO3-N drinking water standard was calculated to be exceeded.  相似文献   

12.
We investigated the denitrification activity and the distribution of the denitrifying bacteria of a boring survey site located on a volcanic plateau, where the geological profiles from surface to deep subsurface soil at the groundwater level had been examined. There were differences between the water quality in the Ito pyroclastic flow deposit (Shirasu) layer (44.2 to 54.5 m) and that in the Osumi pumice fall deposit (Pumice) layer (below 54.5 m) corresponding to the impermeable layer of unconfirmed groundwater: The nitrate concentration was less than 1 mg kg−1 in the Shirasu layer and more than 10 mg kg−1 in the Pumice layer (Kubota et al. 2005). Denitrification activity decreased from the surface to the loam layer and was enhanced in the Shirasu layer and the Haraigawa clay impermeable layer at a depth of 65 m. It was observed that the highest potential denitrification activity (103 ng-N2O d−1 g−1) in the impermeable layer was almost equal to that of a Kuroboku surface soil with slurry application. Viable counts of the sonic-samples, which indicated the presence of bacterial group with soil particles attached, increased in the impermeable layer. The ratios of viable or denitrifying bacterial counts in the sonic-samples to those in the wash-samples were significantly higher in the impermeable layer than those in the surface layer. These results suggest that the hydrogeological conditions enhanced the denitrification activity in the impermeable layer, the niches of which might be relatively anaerobic and have a sufficient supply of substrates to enable the denitrifying bacterial populations to multiply.  相似文献   

13.
(pp. 17–24)
A trial calculation was performed of the environmental nitrogen-assimilation capacity and the amount of nitrogen input based on various statistical data, which were compiled from each city, town and village in Hokkaido prefecture. The relationship between the excess quantity of nitrogen, after nitrogen input, and the environmental nitrogen-assimilation capacity and the nitrate-nitrogen concentration of the groundwater was considered.
Environmental nitrogen-assimilation capacity = nitrogen output by the crops + acceptable level of residual nitrate in the soil profile.*
*It is calculated by the amount of nitrate precipitation evapotranspiration ×10 mg L−1.
  • 1) 

    The average value of the environmental nitrogen-assimilation capacity in Hokkaido Prefecture was observed to be 183 kg ha−1. The maximum and minimum values of the environmental nitrogen-assimilation capacity were 308 kg ha−1 and 94 kg ha−1, respectively. When the average value of the environmental nitrogen-assimilation capacity with respect to main agricultural land use was compared across municipalities, it was largely in the following order · grassland (218 kg ha−1), upland (169 kg ha−1), and paddy land (157 kg ha−1).

      相似文献   

14.
Abstract. Soil samples from a 32-year grassland field experiment were taken from 0–5, 5–10, and 10–15 cm soil depths in February 2002. Plots received annual treatments of unamended control, mineral fertilizer, three rates of pig slurry and three rates of cow slurry, each with six replicates. Samples were analysed for cation exchange capacity (CEC), exchangeable cations (Na+, K+, Ca2+, Mg2+), pH and Olsen P. Exchangeable sodium percentage (ESP) was calculated as a sodicity indicator. Mean ESP was generally greater for slurry treatments than the control, with a trend of increasing ESP with application rate. This was particularly marked for cow slurry. At 0–5 cm depth ESP increased from 1.18 in the control to 1.75 at the highest rate of pig slurry and 5.60 at the highest rate of cow slurry. Similar trends were shown for CEC, exchangeable Na+, K+ and Mg2+, Ca2+ and Olsen P. The build-up of soil P due to slurry applications, together with this combination of physical and chemical factors, may increase the risk of P loss to surface waters, particularly from soils receiving high rates of cow slurry.  相似文献   

15.
The β-ketoadipate pathway is the major route for degradation of aromatic compounds by various soil microorganisms. Protocatechuate 3,4-dioxygenase, a key enzyme of this pathway and which is encoded by pcaGH genes, catalyses the ring cleavage of protocatechuate. Microorganisms harbouring pcaGH genes are widely distributed in the environment but little is known about their relative abundance within the total microflora. Hence, this paper reports the development of a real-time PCR assay to quantify the bacterial pcaH sequence that encodes the β sub-unit of the protocatechuate 3,4-dioxygenase. This real-time PCR assay was linear over seven orders of magnitude with a calculated efficiency of 99% and sensitive up to 102 copies of the pcaH sequence per assay. Real-time PCR analyses performed on six soils with different physico-chemical properties, revealed pcaH densities ranging from 103 to 104 copies of pcaH  ng−1 of soil DNA, which corresponded to approximately 0.2–10.9% of the total bacterial community. The sequencing of real-time PCR amplicons yielded 48 deduced amino acid sequences that exhibited 44–100% identity to known bacterial PcaH sequences, thereby confirming the accuracy of this real-time PCR assay.  相似文献   

16.
Methanogenic archaeal communities inhabiting the paddy field soils in the Kojima Bay polder were investigated using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), real-time PCR and sequencing analyses. Soil samples of the plow and subsoil layers were collected in 2006 from four paddy fields that were reclaimed between 1692 and 1954. The DGGE band patterns of the targeted 16S rRNA genes amplified from the extracted DNA from the samples were different from the patterns from the paddy field soils in diluvial and alluvial areas. The numbers of targeted 16S rRNA genes, which were involved with methanogenic archaeal and other archaeal sequences, were approximately 107–108 and 106 g−1 dry soil in the plow and subsoil layers, respectively. Sequences of methanogenic archaeal 16S rRNA genes belonging to Methanocellales (Rice cluster I), Methanosarcinales and Methanobacteriales were obtained from the major DGGE bands. Whereas sequences in Methanomicrobiales, which were predominant methanogens in the diluvial and alluvial paddy fields, were not recovered. Known halophilic and methylotrophic methanogens, which are characteristic of saline and marine environments, were not detected. These results indicate that distinctive methanogenic archaeal communities have developed in the paddy field soils in the Kojima Bay polder.  相似文献   

17.
Abstract. The saline–sodic soils of the dryland Songnen Plain in northeast China are only slowly permeable to fresh water because of their large content of montmorillinite clay and sodium bicarbonate. Use of slightly saline groundwater containing adequate dissolved calcium and magnesium for leaching and reclamation can potentially prevent dispersion of the clay soil particles during treatment. Amelioration was evaluated using shallow, mildly saline groundwater to irrigate sorghum–corn rotations in a two-year field experiment. After two growing seasons during which a total of 400 mm of leaching water was applied, in addition to some supplemental irrigation water, the average electrical conductivity (ECe) of the top 1.2 m of the soil profile decreased from 14.5±3.5 to 2.7±0.2 dS m−1, and the sodium absorption ratio (SARe) decreased from 35.3±4.1 to 10.1±2.5 (meq L−1)0.5. The soil physical properties were improved: infiltration rate with mildly saline groundwater increased from 12.1 to 42 mm h−1. Salinity changes in the top 1.2 m of soil layers after 700 mm of leaching produced no further improvement. Crop yields produced on plots undergoing amelioration increased by 64–562% compared with the rainfed control. The improved soil conditions after leaching resulted in 59–548% greater crop yields.  相似文献   

18.
Carbon and nitrogen stocks in the soils of Central and Eastern Europe   总被引：6，自引：1，他引：6  
Abstract. Soil organic carbon and total nitrogen stocks are presented for Central and Eastern Europe. The study uses the soil geographic and attribute data held in a 1:2 500 000 scale Soil and Terrain (SOTER) database, covering Belarus, Bulgaria, Czech Republic, Estonia, Hungary, Latvia, Lithuania, Moldova, Poland, Romania, the Russian Federation (west of the Urals), Slovakia, and Ukraine. Means and coefficients of variation for soil organic carbon and total nitrogen are presented for each major FAO soil grouping. The mean content of organic carbon, to a depth of 1 m, ranges from 3.9 kg C m–2 for coarse textured Arenosols to 72.9 kg C m–2 for poorly drained Histosols. Mean carbon content for the mineral soils, excluding Arenosols, is 15.8 kg C m–2. The top 1 m of soil holds 110 Pg C (Pg=1015 g), which corresponds to about 7% of the global stock of soil organic carbon. About 44% of this carbon pool is held in the top 0.3 m of the soil, the layer that is most prone to be changed by changes in soil use and management. About 166 million ha in Central and Eastern Europe have been degraded by compaction, erosion of topsoil, fertility decline and crusting. The achievable level of carbon sequestration for these soils, upon adoption of 'best' management practices or restorative measures, is estimated.  相似文献   

19.
We investigate the effect of Al on K+ uptake by rice roots. Potassium-38 (38K), a positron emitting nuclide (the half-life: 7.61 min), was used to trace K+ behavior. When a rice root was treated with 10μM Al for 24 h, the uptake of 38K in the root was increased in the range of 1 to 2 cm from the root tip compared with that of the control sample. Because the root continued to grow without showing any damage of plasma membrane during the Al treatment, it was suggested that the 38K uptake was not occurred through diffusion into the cells. The uptake of 38K in both treatments, with/without Al, was decreased by VO43- (inhibitor of H+-ATPase on plasma membrane) and DNP (H+ ionophore) treatment, which suggested that the K+ uptake was performed through an active transport, such as H+:K+ transport or H+ gradient promoted by an Al treatment.  相似文献   

20.
Ovomucin glycopeptide (OGP) was prepared by size exclusion chromatography after Pronase digestion of hen egg ovomucin, and the binding of OGP to foodborne pathogens (Bacillus cereus,Clostridium perfringens, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, and Staphylococcus aureus) was investigaed. Binding assays with biotinylated bacteria as probes in microtiter plates showed that OGP bound to only E. coli O157:H7 among these foodborne pathogens. Periodate treatment markedly reduced the binding ability, indicating that E. coli O157:H7 bound to carbohydrate moieties of OGP. Lectin blot analysis with Maackia amurensis (MAA) and Sambucus nigra (SNA), which are specific for oligosaccharides containing sialic acid, revealed their binding sites in OGP were similar to the E. coli O157:H7 binding sites that were probed with biotinylated E. coli O157:H7 after Western blotting of OGP. Sialydase treatment of OGP abolished its ability to bind E. coli O157:H7, demonstrating that sialic acid played an important role in the binding. These results suggest that OGP has E. coli O157:H7-specific binding sites that consist of sialic acid. On the basis of these properties, OGP has the potential to be an ingredient with a protective effect against E. coli O157:H7 infection and to be a novel probe for the detection of E. coli O157:H7 in the food hygiene field.  相似文献   

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