铁皮石斛EST-SSR分子标记的开发     

付涛  胡仲义  何月秋  李文  林立 《核农学报》2017,31(4)

为了开发铁皮石斛EST-SSR分子标记,利用SSRIT软件对铁皮石斛近缘物种-金钗石斛的15 383条EST序列进行SSR位点查找,利用Primer 5和Oligo 7软件进行引物设计和评价,之后经PCR扩增和琼脂糖凝胶电泳对引物进行初步筛选,并通过聚丙烯酰氨凝胶电泳对所筛选的引物进行有效性检测。结果表明,SSRIT软件共查找到370条SSR位点序列,共有379个SSR位点,候选SSR位点出现的频率为2.46%。二核苷酸、三核苷酸和六核苷酸重复是最主要的重复类型,分别占41.42%、26.91%和27.44%,其中二核苷酸重复中AG/CT(20.84%)和GA/TC(18.73%)最丰富;三核苷酸重复中TCT/AGA(4.22%)和GA/TC(3.96%)最丰富;而六核苷酸重复基元较多。EST-SSR平均长度为24 bp,平均每8.5条EST序列包含1个SSR位点。设计的106对EST-SSR引物中有50对能扩增出理想的PCR产物,引物有效扩增率为47.17%,其中有43对能够扩增出多态性条带,占可扩增引物的86%。本研究开发的43对引物为铁皮石斛遗传多样性分析、种质鉴定、遗传图谱构建和功能基因研究等提供了重要的参考价值。  相似文献   

三种人参属植物的EST-SSR信息分析及其在三七中的应用     

杨维泽  金航  崔秀明  沈涛  杨美权  张金渝 《广西农业生物科学》2011,30(1):62-71

为了解人参属植物EST中SSR分布特点及其在三七SSR标记中的应用,利用生物信息学方法,用primer3软件对dbEST数据库中人参属植物人参、西洋参和三七的EST序列进行搜索,发现人参属植物EST-SSR出现频率为11.54%,平均每4.39kb出现1个SSR。人参属植物单核苷酸重复基元占主导地位,其次为三核苷酸重复基元和二核苷酸重复基元,分别占总SSR的54.48%、17.31%和16.36%。人参属EST-SSR的优势类型为A/T和AT/TA,分别占54.73%和8.21%。根据人参属植物EST中的SSR设计48对引物,在合适的PCR扩增体系下,用5个三七样品的DNA为模板进行PCR扩增,引物有效扩增率85.42%,其中多态性引物占可扩增引物的70.73%。结果表明,利用人参属EST序列开发三七EST-SSR标记是可行的。  相似文献   

菠菜性别相关EST-SSR标记的开发及应用     

邓传良  高俊  曹莹  秦瑞云  高武军  卢龙斗 《广西农业生物科学》2012,(5):467-472

为了明确菠菜EST序列中SSR的总体特点,开发菠菜EST-SSR引物;为利用EST-SSR引物进行菠菜性别相关特异序列的克隆奠定基础,本文从NCBI上获得1093条EST,利用在线软件SSRIT检测所含SSR序列,并进行分析。共检索出68条SSR序列,分布于64条EST中,检出率为6.22%,包括22种重复基元。其中二核苷酸重复基元的EST-SSR占主导地位,占总SSR数目的32.3%。利用在线引物设计软件Primer3.0设计了7对EST-SSR引物,在适合的PCR反应体系下,分别以雌、雄菠菜DNA基因组为模板,对设计的EST-SSR引物进行筛选,结果显示以EST序列HS097148设计的一对引物从菠菜雌雄基因组中扩增出一条雄性特异的条带,表明通过菠菜EST-SSR引物获得菠菜性别相关特异序列是可行的。  相似文献   

银杏EST序列中微卫星的分布特征     

樊洪泓  李廷春  李正鹏  林毅  蔡永萍 《广西农业生物科学》2009,(5):869-873

本文利用从NCBI下载的21590条银杏EST序列,分析了银杏（表达序列标签微卫星）EST-SSR在银杏EST序列的分布和比较了在不同长度EST序列中的SSR特性。在剔除冗余和低质量序列后,得到总长为5708.385kb的无冗余EST序列7961条,发现了405个EST序列（5.09%）含有475个SSR,长度400~1000bp的EST序列含SSR位点数为445个,占SSR总数的93.68%。二核苷酸和三核苷酸基元类型是银杏EST-SSR的主要类型,分别占SSR总数的73.89%和24.00%,最常见的SSR基元是：（AT）n、（AG）n、（AC）n、（AAG）n和（AAT）n。通过对银杏EST序列中SSR位点信息的发掘分析,为有针对性地设计EST-SSR引物,开发银杏EST-SSR分子标记奠定基础。  相似文献   

菊花EST-SSR标记开发及其对万寿菊的可转移性研究     

张华丽  王涛  宋利娜  董爱香  辛海波  义鸣放  赵金华 《核农学报》2016,(1):42-49

为开发菊花EST-SSR标记并用于万春菊资源分析,研究了菊花的EST-SSR特征、标记开发及其在万寿菊上的可转移性。结果表明,菊花EST-SSR出现频率为5.35%,平均每10.64 kb存在1个SSR位点。在这些SSR位点中,共有83种重复基元,2-6核苷酸重复类型分别为10、40、14、5和14种。以14个菊花品种DNA为模板,22对菊花EST-SSR引物中14对可以扩增到清晰且稳定的PCR产物,共检出49个位点,平均每对引物可扩增出3.5条多态性片段,平均多态性信息含量(PIC)为0.751。在14对有效引物中,8对可以在待检万寿菊样品中扩增到清晰稳定的条带,可转移率为57.14%。综上所述,菊花以三核苷酸重复类型占主导,六核苷酸重复类型高于其它植物种类,菊花EST-SSR引物多态性信息含量处于较高水平。本研究可为万寿菊分子标记辅助育种奠定科学基础。  相似文献   

大白菜表达序列标签SSR标记分析*   总被引：18，自引：2，他引：18  

葛佳  谢华  崔崇士  洪剑明  马荣才 《农业生物技术学报》2005,13(4):423-428

对大白菜（Brassica campestris L．ssppekinensis）13007个EST序列进行拼接共得到7714个片段重叠群（contig），其中10．4％（890个）非冗余EST含有SSR标记（EST-SSR），标记间平均间隔距离为3．9kb，单、双和三核苷酸重复序列大约各占1／3左右。CDS所包含的三核苷酸重复序列最多，而且AAG／CTT重复序列的含量最高。通过同源性比较分析，获得功能已知的SSR-ESTs774个，共计含有846个SSR，其中50．2％位于5＇-UTR，31．4％位于3＇-UTR，18．4％位于CDS。实验选取123个SSR座位设计引物，利用芜菁花叶病毒（Turnip mosaic virus，TuMV）的高抗和高感大白菜F6代自交系材料各2份，分析EST-SSR标记多态性。结果表明，其中37个标记可进行有效扩增，但是没有表现出多态性，23个标记（18．7％）至少在1份材料中具有多态性。对这些EST-SSR标记在大白菜和其它芸薹属植物的系统发育关系分析、遗传作图和基因定位的应用进行了讨论。  相似文献   

香菇表达序列标签(EST)的SSR分析及EST-SSR引物的筛选     

林范学程水明  李安政林方灿 《农业生物技术学报》2007,15(2)

从真菌基因组计划网站(FGP)和NCBI网站数据库下载了总长度为8.1Mb的11150条香菇的EST(包括香菇的10条cDNA)序列，通过SSRhunter 1.3软件结合手工查找，从中发现2.83％即316条EST含有一共469个SSR.，平均每17.3kb出现一个EST-SSR。在所有EST-SSR中，三碱基和六碱基SSR出现最多，分别占EST-SSR总数的38.00％和20.00%，出现较多的基元为(A)n、(T)n、(GA)n、(AG)n、(TGA)n、(GAT)n和(TCTTT)n，占所有EST-SSR的35.39％。利用Oligo 6.0软件设计了51对EST-SSR引物，并选用其它物种的24对引物，对香菇菌株进行了PCR扩增。实验结果表明，设计的51对引物有39对具有明显的扩增产物，选用的其它物种的24对引物均没有清晰可见的特异性扩增产物。部分有扩增产物的EST-SSR引物具有多态性。  相似文献   

人参EST-SSR标记的建立   总被引：3，自引：0，他引：3  

杨成君  王军  穆立蔷  李绍臣  刘关君  胡长群 《农业生物技术学报》2008,16(1):114-120

从7055条人参EST序列中共搜索出791个SSR。根据引物设计标准,设计了68对EST-SSR引物。在合适的PCR反应体系下,分别以人参品种集安长脖、抚松二马牙的DNA为模板,对设计的EST-SSR引物进行筛选,发现有43对EST-SSR引物能扩增出产物。在9个人参品种、2个西洋参品种和2个刺五加品种中进一步对这些可扩增的引物对进行多态性检测,发现有26对引物显示多态性,占可扩增引物的60.47%,占设计引物总数的38.23%。本研究结果表明,根据人参EST建立EST-SSR标记是一种行而有效的的方法。  相似文献   

大白菜硫代葡萄糖甙合成基因SSR标记的开发     

巩红影  罗双霞  顾爱侠  王彦华  赵建军  申书兴 《农业生物技术学报》2014,22(10)

硫代葡萄糖甙(glucosinolates,GS)是一种含氮、硫的重要植物次生代谢产物,是十字花科蔬菜风味的主要来源。本研究根据拟南芥(Arabidopsis thaliana)和大白菜(Brassica rapa ssp.pekinensis)基因组序列信息,比对GS生物合成相关基因的序列信息,发现大白菜中与GS含量相关的候选基因102个,分布于10条染色体上。拟南芥GS合成基因在大白菜中的同源基因个数分别为:零拷贝基因8个、单拷贝基因13个、2拷贝基因17个、2个以上多拷贝基因14个。通过FASTPCR6.0软件分析目标基因上下游序列各5kb,共开发出237个简单重复序列(SSR)位点,16个基因没有发现SSR位点,72个基因存在1~4个SSR位点,5个基因存在5个SSR位点,4个基因存在6个SSR位点,4个基因存在7个SSR位点,1个基因存在8个SSR位点。所开发的SSR位点中,共发现4种重复类型:单核苷酸重复最多,共122个,占SSR总数的51.4%;其次是二核苷酸重复类型,共48个,占SSR总数的20.3%;三核苷酸重复类型最少,共7个,占SSR总数的3.0%,另外60个SSR无明显的重复类型。具有SSR位点的86个基因中,77个基因可以设计SSR特异引物,其中49对特异引物在供试的75份大白菜高代自交系中得到有效扩增。其中16对引物具有多态性,占全部引物的20.8%;18对引物无多态性,占全部引物的23.4%;15对引物杂带多,占全部引物的19.5%;另外28对引物无扩增产物,占全部引物的36.4%。最终11对获得了清晰多态性扩增产物,共检测出26个等位基因。本研究为分子标记辅助选择培育高有益GS成分的大白菜新品种提供了基础资料,进而加快大白菜营养品质育种的进程。  相似文献   

大白菜简单序列重复(SSR)和插入/缺失(InDel)标记的开发及通用性分析     

仪泽会  卢有飞  郭晓芹  惠麦侠  张鲁刚  张明科 《农业生物技术学报》2012,20(12):1398-1406

为探讨大白菜基因组序列中SSR位点的分布规律并开发SSR引物,利用SSRHunter软件对大白菜A10(16899818～17299817)的DNA序列进行简单序列重复(SSR)位点查找,共得到394个SSRs,平均每1.02kb出现1个SSR。二核苷酸和三核苷酸重复是最主要的SSR类型,分别占79.44%和18.78%。为了提高SSR标记开发的准确性和通用性,对检索得到的含SSR位点的序列进行了同源比对,选取符合条件的15条SSR序列并设计引物;依据Blast过程中发现的在SSR位点不存在差异而在其侧翼序列中存在插入/缺失(InDel)差异的序列,设计了19条InDel引物。用34对SSR及InDel引物在6个大白菜(Brassica rapassp.pekinesis)材料中进行多态性研究,发现28对引物能扩增出理想的PCR产物,有效扩增率为82.35%,其中27对引物具有多态性,多态性比率为79.41%。为验证SSR引物的真实性,随机对4对SSR引物的部分白菜扩增片段进行了测序,发现100%的片段具有相应的SSR位点。28对SSR和InDel引物在甘蓝(B.oleracea)、油菜(B.napus)和萝卜(Raphanus sativus)品种的有效扩增率分别为85.71%、100%和77.78%,说明新开发的SSR和InDel标记具有较好的多态性和通用性。利用6对引物分析了48份十字花科种质的遗传多样性,结果表明48份材料被明显地区分成白菜和甘蓝组、萝卜组、油菜组3大类群,与传统分类一致。大白菜SSR和InDel标记的开发对于十字花科种质亲缘关系及遗传多样性分析具有重要的应用价值。  相似文献   

毛竹PheDof2转录因子克隆及表达分析     

刘俊  程占超  郑慧芳  蔡苗苗  彭立鑫  白羽聪  宋华建  高健 《核农学报》2021,35(3):586-594

Dof蛋白是单锌指结构蛋白家族之一,在植物生长发育和非生物胁迫中发挥重要作用。为了探索Dof转录因子在毛竹中的功能及表达特征,本研究以毛竹实生苗为材料,采取反转录PCR(RT-PCR)技术克隆得到PheDof2基因的cDNA 序列,对其进行生物信息学初步分析,并检测其在毛竹不同高度笋、花发育,及不同胁迫处理下的表达情况。序列分析结果表明,PheDof2开放阅读框(ORF)为1 614 bp,编码527个氨基酸,分子量和等电点分别为53.20 kDa和5.37,具有典型的zf-dof结构域;生物信息学分析结果显示,PheDof2含有两个外显子,一个内含子,启动子序列中存在多个非生物胁迫和光响应元件。蛋白进化结果表明,PheDof2与二穗短柄草亲缘关系最近,相似性为79.05%。荧光定量PCR检测结果显示,PheDof2在毛竹叶片中表达量最高,其次是根,在茎中表达量最低,参与毛竹笋的快速生长及花的发育,说明该基因具有组织表达特异性。在干旱处理的根中,处理6 h 时,PheDof2的表达水平出现峰值;在幼茎中,处理24 h时PheDof2的表达量降为最低值。在干旱和脱落酸(ABA)处理的叶片中,PheDof2均在处理6 h时降为最低值。结果表明,PheDof2参与干旱胁迫及ABA介导的信号转导途径。本研究结果为毛竹抗逆育种及PheDof2基因的功能研究提供了理论基础。  相似文献   

毛竹入侵森林对固氮微生物群落结构和丰度的影响     

周燕  刘彩霞  徐秋芳  梁辰飞  陈俊辉  秦华  李永春 《植物营养与肥料学报》2018,24(4):1047-1057

【目的】 毛竹入侵能够对生态系统的各个方面产生影响,包括森林生态系统氮循环。生物固氮是森林生态系统氮循环的重要一环,研究毛竹入侵过程中土壤固氮微生物的变化,可为毛竹入侵对生态功能影响的评价提供理论依据。 【方法】 选取天目山自然保护区的三个毛竹入侵带 (青龙山、石门洞和进山门),分别在三个入侵带中沿毛竹入侵方向采集毛竹纯林、毛竹与原林混交林以及原林地的表层土壤,分析pH、有机碳、碱解氮、有效磷和速效钾等土壤化学性质,应用基于nifH功能基因的末端限制性片段长度多态性 (T-RFLP) 和荧光定量PCR (qPCR) 技术,分析土壤固氮微生物群落结构和丰度的变化。 【结果】 毛竹入侵后土壤化学性质呈现三种不同情况,在三个入侵带中总体上升的有pH、有机碳、碱解氮、有效磷,下降的为速效钾,而硝态氮的变化均不显著,总体上,土壤养分含量在毛竹入侵后有所上升。土壤固氮微生物的丰度随毛竹入侵过程降低,其中进山门入侵带的变化显著 (P < 0.05)。土壤固氮微生物T-RFLP的结果显示,48 bp在三个入侵带中均为优势片段;不同的T-RFs在毛竹入侵过程中变化各异,有的片段在毛竹入侵后消失,有的片段在毛竹入侵后出现,有的片段变化不显著。土壤固氮微生物群落的Shannon指数和均匀度指数随毛竹入侵降低,Simpson指数相反,这些指标只在石门洞入侵带的不同林分之间存在显著差异 ( P < 0.05);多响应置换过程分析 (MRPP) 显示,石门洞和进山门入侵带毛竹入侵前后土壤固氮微生物群落结构有显著变化 ( P < 0.05);冗余分析 (RDA) 结果表明,土壤性质对固氮微生物群落变化的解释率普遍较低 (低于30%),三个入侵带显著影响土壤固氮微生物群落变化的土壤化学性质各不相同,并且三个入侵带的样点在RDA坐标图中分布格局并不统一。 【结论】 即使同一自然保护区的三个毛竹入侵带,土壤固氮微生物群落随着入侵过程的变化并不一致,母岩和原有植被产生的综合作用导致土壤固氮微生物群落发生变化,但需结合更多的因素进行进一步地探讨。   相似文献   

长期种植毛竹林土壤丛枝菌根真菌群落演变趋势     

牛利敏  秦华  徐秋芳  邬奇峰  苗倞婧  彭定聪 《土壤学报》2017,(3):722-734

为揭示长期种植毛竹林土壤丛枝菌根(Arbuscular mycorrhizae,AM)真菌群落演变特征,通过磷脂脂肪酸(Phospholipid fatty acid,PLFA)和Illumina Miseq测序平台研究了AM真菌生物量及群落结构的演变趋势。结果表明,长期种植毛竹林土壤养分含量总体呈下降趋势,球囊霉素相关土壤蛋白(Glomalin-related soil protein,GRSP)含量及AM真菌生物量也显著降低(p0.05),其中易提取态球囊霉素相关土壤蛋白(Easily extractable glomalin-related soil protein,EE-GRSP)含量与有机质、碱解氮、速效钾显著正相关(p0.05),而AM真菌菌丝生物量(16:1ω5 PLFA)与碱解氮极显著正相关(p0.01)。长期种植毛竹林显著降低了土壤2~0.25 mm大团聚体比例(p0.05),且与AM真菌菌丝生物量极显著正相关(p0.01)。测序结果表明,毛竹林土壤AM真菌以球囊霉属(Glomus)为优势种群,其次为无梗囊霉属(Acaulospora),长期种植毛竹后土壤球囊霉属相对丰度显著增加而无梗囊霉属显著降低(p0.05)。非度量多维尺度转换排序(Non-metric multidimensional scaling,NMDS)分析显示,对照马尾松林与不同种植年限毛竹林土壤AM真菌群落显著区分(p=0.001),土壤含水量(p=0.005)、碱解氮(p=0.001)、有效磷(p=0.014)对AM真菌群落结构变异具有重要贡献。长期种植毛竹显著降低了AM真菌生物量、球囊霉素相关土壤蛋白含量以及2~0.25 mm大团聚体比例,并改变了AM真菌群落结构,不利于土壤碳固存和维持生态系统稳定。  相似文献   

毛竹竹鞭内生细菌的特征及16S rDNA多样性分析     

袁宗胜  ;刘芳  ;张国防 《广西农业生物科学》2014,(5):1019-1024

本研究利用传统的细菌分离方法,结合16S rDNA序列分析对毛竹竹鞭内生细菌的特征和多样性进行了分析。从福建省武夷山、将乐、长汀毛竹竹鞭中分离到34株内生细菌,初步归属于14属,20种。来源于不同地区的毛竹竹鞭内生细菌组成存在较大差异,其优势菌群为产碱杆菌属（Alcaligenes）和葡萄球菌属（Staphylococcus）。  相似文献   

The carbon storage in moso bamboo plantation and its spatial variation in Anji County of southeastern China   总被引：1，自引：0，他引：1  

Weijun Fu  Peikun Jiang  Keli Zhao  Guomo Zhou  Yongfu Li  Jiasen Wu  Huaqiang Du 《Journal of Soils and Sediments》2014,14(2):320-329

Purpose

Forest ecosystems play an important role in sequestering carbon in their biomass and soils. Moso bamboo plantations, as a special forest type, are mainly distributed in southern China. There is little information about the carbon storage in moso bamboo plantations, it is now important to better understand the moso bamboo’s carbon sequestration. The main objectives of this study were to investigate the spatial variation of aboveground biomass carbon (AGC) and soil organic carbon (SOC) in moso bamboo plantations and to compare carbon storage in moso bamboo plantations under different management options.

Materials and methods

A total of 73 moso bamboo plots were selected in Anji County, which is a famous “bamboo town” in northwest Zhejiang province, China. The diameter at breast height and the age of each moso bamboo in the selected plots were measured in order to calculate the AGC. SOC was analyzed using sulfuric acid–potassium dichromate solution. One-way ANOVA was applied to analyze the significant difference of AGC and SOC under different management options. Geostatistics and geographical information were used to study the spatial dependence characteristics of AGC and SOC.

Results and discussion

The AGC values were very variable, ranging from 9.92 to 38.70 Mg?ha^?1, with an average of 20.85 Mg?ha^?1. The SOC values were from 34.8 to 176.17 Mg?ha^?1. Both the AGC and SOC values were followed normal distributions. Moso bamboo plantations contributed about 16.5 % of total forest biomass carbon in Zhejiang Province, indicating its important influence on regional carbon budget. Geostatistical analysis revealed that the AGC had moderate spatial autocorrelation. A nested model (a spherical model with a Gaussian model) was chosen to describe the variogram. Spatial patterns for AGC were found in Anji County, with relatively high AGC values were found in the southwestern part of Anji County, and low values were located in the eastern and central parts of the county. While no clear spatial autocorrelation trend was observed in the semivariogram of SOC, indicating a random distribution pattern for SOC in the study area. Meanwhile, the Pearson’s correlation between AGC and SOC in bamboo plantation was weak (r?=?0.064, p?=?0.496), due to moso bamboo’s special growth process and different management options by human beings.

Conclusions

In this study, moderate spatial dependency was found for AGC, while the spatial autocorrelation of SOC was poor. In moso bamboo forest ecosystem, SOC was mainly stored at the top 40 cm layer. Management options were proved to be an important factor for carbon sequestration. Extensive management is an efficient way to increase carbon stock, compared to moderate and intensive management. With the rapid increase of plantation area, moso bamboo ecosystem will continue to play an important role in regional carbon budget.  相似文献   

毛竹CesA基因鉴定及其表达调控研究     

王新悦  朱成磊  杨克彬  李广柱  李真  袁婷婷  高志民 《核农学报》2022,36(4):706-715

纤维素合成酶(CesA)是纤维素合成的关键酶。为探究毛竹(Phyllostachys edulis)中CesA基因的分子特征、表达模式及其调控关系,本研究采用生物信息学方法对毛竹CesA基因家族成员进行系统分析,基于毛竹RNA-Seq数据和实时荧光定量PCR(qRT-PCR)技术分析CesA在毛竹组织中的表达模式,借助酵母单杂交技术验证转录因子MYB对CesA的调控作用。结果表明,在毛竹中共鉴定21个CesA(PeCesA1~PeCesA21),分别含有8~14个内含子;共线性分析发现19个PeCesAs存在片段复制,且Ka/Ks值均小于1.0。PeCesAs编码蛋白长度为904~1 093 aa,分子量在101.10~123.63 kDa之间,理论等电点为5.95~8.60;亚细胞定位预测所有PeCesAs都定位在质膜上;保守基序分析发现,PeCesAs共含有20个保守基序,均含有CesA家族的特征基序D、D、D和QxxRW。系统发育分析结果表明,毛竹等4个物种的CesA家族52个成员聚类成7个分支。RNA-Seq数据分析结果表明,PeCesAs(PeCesA1/5/10/11/17)在根以及不同高度笋的中部和基部的表达量存在明显差异,而且PeMYB35和PeMYB37均与PeCesA1/5/10/11/17存在较强的共表达关系。qRT-PCR结果进一步证实,随着毛竹笋高度的增加,PeMYB35与PeCesA1/5/10/11/17呈现相似的表达趋势。此外,PeCesA1/5/10/11/17的启动子序列中均包含MYB转录因子的结合位点GAMYB,酵母单杂结果证实PeMYB35能够与PeCesA1启动子中GAMYB元件相结合,可能会进一步调控基因的表达。该结果为研究毛竹中纤维素的生物合成机制提供了参考依据。  相似文献   

Phyllostachys edulis (moso bamboo) rhizosphere increasing soil microbial activity rather than biomass     

Zhang  Wenyuan  Liu  Shun  Zhang  Manyun  Li  Yinan  Sheng  Keyin  Xu  Zhihong 《Journal of Soils and Sediments》2019,19(7):2913-2926

Purpose

Rhizosphere and fertilization might affect soil microbial activities, biomass, and community. This study aimed to evaluate the impacts of Phyllostachys edulis (moso bamboo) rhizospheres on soil nutrient contents and microbial properties in a moso bamboo forest with different fertilizer applications and to link soil microbial activities with abiotic and biotic factors.

Materials and methods

The experiment included three treatments: (1) application of 45% slag fertilizer (45%-SF); (2) application of special compound fertilizer for bamboos (SCF); and (3) the control without any fertilizer application (CK). Simultaneously, bulk soils and 0.5, 2.5, 4.5, and 6.5-year-old (y) bamboo rhizosphere soils were selected. Soil nutrient contents were analyzed. Microbial activities were evaluated based on the activities of soil enzymes including β-glucosidase, urease, protease, phosphatase, and catalase. The total microbial biomass and community were assessed with the phospholipid fatty acids (PLFAs) method.

Results and discussion

In the CK and SCF treatments, organic matter contents of rhizosphere soils were significantly higher than those of bulk soils. Soil β-glucosidase, urease, protease, phosphatase, and catalase activities in rhizosphere soils were higher than those of bulk soils, with the sole exception of β-glucosidase of 0.5 y rhizosphere soil in the 45%-SF treatment. Compared with the CK treatment, fertilizer applications tended to increase soil total PLFAs contents and changed soil microbial community. Moso bamboo rhizospheres did not significantly increase the total microbial biomass. In the SCF treatment, the Shannon index of bulk soil was significantly lower than those of rhizosphere soils.

Conclusions

Our results suggested that both rhizospheres and fertilizer applications could change the soil microbial community structures and that moso bamboo rhizosphere could increase microbial activity rather than biomass in the forest soils with different fertilizer applications.

  相似文献   

Analysis of genetic diversity of Ruthenia Medic (Medicago ruthenica (L.) Trautv.) in Inner Mongolia using ISSR and SSR markers     

Hong-yan Li  Zhi-yong Li  Li-yan Cai  Wen-gui Shi  Fu-gui Mi  Feng-ling Shi 《Genetic Resources and Crop Evolution》2013,60(5):1687-1694

Ruthenia Medic is tolerant to drought, cold, high salinity, resistance to trampling and high quality features. Inter-simple sequence repeat (ISSR) and simple sequence repeat (SSR) molecular markers were employed for the first time to access the genetic diversity and relationships of 30 wild Ruthenia Medic accessions obtained from Inner Mongolia in the present study. A total of 94 bands were amplified by ten ISSR primers, of which 83 (88.5 %) were polymorphic, and 57 polymorphic bands (80.4 %) were observed in 69 bands amplified by ten SSR primers. Shannon’s information index (I = 0.487), and average expected heterozygosis (He = 0.329) generated by ISSR primer were higher than that of SSR analysis (I = 0.372, He = 0.231). The study indicated that ISSR were more effective than SSR markers for assessing the degree of genetic variation of Ruthenia Medic. UPGMA cluster analysis revealed inconsistencies in the clustering patterns, as the Mantel’s test between the dendrograms for ISSR and SSR data indicated a poor fit for the ISSR and SSR data types (r = 0.0970). Whereas the pattern of clustering of the genotypes remained relatively the same in ISSR and combined data of ISSR and SSR. The results of principal components analysis also supports their UPGMA clustering. These results have an important implication for Ruthenia Medic germplasm characterization, improvement, and conservation.  相似文献   

Study on phosphorus loss and influencing factors in the water source area   总被引：2，自引：1，他引：1  

《国际水土保持研究(英文)》2022,10(2):324-334

Maintaining drinking water security is a global issue, and phosphorus is a limiting factor affecting drinking water quality. Hence, this study took Fushi Reservoir as a test area, and set up field runoff observation plots around the reservoir catchment, which is covered by moso bamboo stands. Through field observation, the vertical variation of phosphorus loss in different stands of moso bamboo was initially studied. The results showed that: (1) For the vertical dimensions (atmospheric rainfall, stemflow, throughfall, surface runoff) from high to low, the loss of total phosphorus (TP) increased, and the proportion of dissolved phosphorus increased from 29.29% (atmospheric rainfall) to 62.76% (surface runoff). (2) Different rainfall factors had various impacts on phosphorus loss at the different vertical levels. The accumulation of rainfall had the greatest impact on surface runoff TP loss, with the correlation coefficient reaching 0.994 (P < 0.01), while surface runoff particulate phosphorus loss was mostly affected by the average rainfall intensity. (3) Modifying the forest structure in water source areas can reduce the loss of TP via stemflow and throughfall, but the effect on surface runoff TP loss is variable. Thus, it is expected that this novel study can serve as a reference for improving the environmental quality of water source areas, and help in reducing phosphorus loss and controlling non-point source pollution.  相似文献   

均匀设计优化毛竹EST-SSR PCR反应体系     

管雨  杨丽  张智俊 《广西农业生物科学》2010,(3):598-602

本研究以毛竹叶片DNA为模板,利用均匀设计U10（54）表,对毛竹EST-SSRPCR反应体系的TaqDNA聚合酶、Mg2＋、dNTPs及引物浓度4因素5个水平进行优化筛选。优化实验结果表明,毛竹EST-SSRPCR的25μL优化反应体系为：10×PCRBuffer（含Mg2＋）2.5μL、TaqDNA聚合酶1.5U,Mg2＋、dNTPs及引物的最适浓度分别是1.0mmol/L、0.1mmol/L、0.48μmol/L;通过梯度PCR试验筛选得到相应引物的最佳退火温度为52.4℃。对优化出的EST-SSRPCR反应体系进行稳定性检测,结果均能获得丰富、稳定、清晰可辨的DNA谱带。该优化体系为利用EST-SSR分子标记对毛竹进行遗传多样性等相关研究工作提供了基础条件。  相似文献