共查询到19条相似文献，搜索用时 187 毫秒
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三种DNA提取方法对检测草莓镶脉病毒稳定性的研究 总被引：2，自引：0，他引：2
草莓成熟叶片中含有大量的多糖、蛋白质、多酚和色素等物质,影响高质量DNA的提取和下游PCR反应的进行。为确定适用于PCR技术检测草莓镶脉病毒(Strawberry vein banding virus,SVBV)的DNA提取方法,从草莓叶片中提取DNA后,对样品中的SVBV进行特异性的PCR检测,比较确定不同DNA提取方法对PCR检测SVBV稳定性的影响。以感染SVBV的草莓叶片为材料,分别采用CTAB试剂盒法、高盐低pH法、改良SDS法提取草莓叶片的总DNA,将不同方法提取的DNA通过紫外分光光度法和琼脂糖凝胶电泳法进行测定,对DNA产量和质量进行分析。结果表明,3种方法均能获得较为完整的DNA片段,用CTAB试剂盒提取的DNA质量最高,改良SDS法提取的DNA质量较低。将不同方法提取的DNA经过PCR反应特异性检测SVBV后,将与目的片段大小一致的PCR扩增产物经序列测定及分析,证明与SVBV的序列相吻合,证明了方法的可靠性,其质量能够满足PCR特异性检测需要。 相似文献
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豆粉基因组DNA不同提取方法的比较 总被引：1，自引：1，他引：0
以市售豆粉为材料,分别采用热解法、异丙醇沉淀法、CTAB法、SDS法、高盐低pH法等以及它们的改良方法提取基因组DNA,并对提取的DNA进行光密度、琼脂糖凝胶电泳和PCR检测。结果表明除改进高盐低pH法外,其它所有方法提取的基因组DNA均可满足PCR检测要求。同时,综合考虑基因组DNA的纯度和浓度,此研究认为豆粉基因组DNA提取方法的优劣依次为：改进热解法、改进异丙醇沉淀法、热解法、异丙醇沉淀法、高盐低pH法、改进CTAB法、CTAB法和改进SDS法。这几种豆粉基因组DNA提取方法都具有操作简单、耗时短、利于快速检测的优点。 相似文献
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一种快速提取小麦基因组DNA的改良CTAB方法 总被引：6，自引：2，他引：4
旨在寻求一种微量、快速提取小麦DNA的方法。以小麦幼叶为材料,用改良CTAB法、改良SDS法、沸水浴法提取小麦叶片总DNA,通过琼脂糖凝胶电泳法、紫外吸收法和PCR检测DNA完整性和纯度。改良CTAB法提取小麦基因组DNA质量和纯度高、无降解,每人每天可以轻松提取200个DNA样品,对转基因植株的PCR检测显示扩增目标条带清晰一致,无假阳性,试验结果理想。改良SDS法所提DNA纯度和浓度虽略不如改良CTAB法,但仍然符合实验要求。沸水浴法提取量少且杂质多,PCR无有效扩增,结果不理想。改良CTAB法提供了一种简便、快速微量小麦DNA提取方法,适用于PCR和其他分子生物学研究。 相似文献
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为满足水稻SSR分析需要大规模DNA样品的要求,以水稻幼苗叶片为材料,采用改良的CTAB法提取水稻基因组DNA.经质量和纯度检测结果显示：改进的方法程序简单,药品用量少,省时省力,且提取DNA的OD260/OD280均在1.8～1.9之间,琼脂糖凝胶电泳和PCR扩增条带清晰、无污染、无降解.表明该方法适用于SSR分析的水稻基因组DNA的提取,所得DNA的质量和纯度完全满足试验要求. 相似文献
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Effect of location, N-fertilization, variety and harvest date on the yield of fermentable sugars of Jerusalem artichoke tops and tubers
In the past, the production of fermentable sugar with Jerusalem artichoke ( Helianthus tuberosus L.) resulted nearly exclusively from tubers.
It should be investigated to what extent the tops can be used beside tubers as a source of fermentable sugars, connected with studies about diverse components of yield and morphological characteristics.
In field trials at three locations in the south-west of Germany in 1983 and 1984 yields of "Total Fermentable Sugars" (TFZ) with Jerusalem artichoke tops up to 6.7 t/ha were achieved according to variety, N-fertilization and harvest date. While harvesting Jerusalem artichoke tubers yields of TFZ up to 8.3 t/ha had been feasible.
The presented correlations between components of yield and morphological plant characteristics refer to interesting mechanisms of reaction and dependence on the formation of yield with this crop. The high yield level of Jerusalem artichoke and its adaptability to different locations also present this crop as an interesting renewable biomass crop for the south-west of Germany. 相似文献
In the past, the production of fermentable sugar with Jerusalem artichoke ( Helianthus tuberosus L.) resulted nearly exclusively from tubers.
It should be investigated to what extent the tops can be used beside tubers as a source of fermentable sugars, connected with studies about diverse components of yield and morphological characteristics.
In field trials at three locations in the south-west of Germany in 1983 and 1984 yields of "Total Fermentable Sugars" (TFZ) with Jerusalem artichoke tops up to 6.7 t/ha were achieved according to variety, N-fertilization and harvest date. While harvesting Jerusalem artichoke tubers yields of TFZ up to 8.3 t/ha had been feasible.
The presented correlations between components of yield and morphological plant characteristics refer to interesting mechanisms of reaction and dependence on the formation of yield with this crop. The high yield level of Jerusalem artichoke and its adaptability to different locations also present this crop as an interesting renewable biomass crop for the south-west of Germany. 相似文献
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S. SCHITTENHELM 《Journal of Agronomy and Crop Science》1996,176(2):103-110
A major production constraint in Jerusalem artichoke ( Helianthus tuberosus L.) is caused by tubers which are not recovered at harvest. Such lost tubers raise a serious weed problem the following season. Winter wheat, oat, spring oilseed rape, sugarbeet, maize and ryegrass were grown in a field which had Jerusalem artichoke as the preceding crop in order to obtain information about their competitive ability and the efficacy of various control measurements against Jerusalem artichoke infestation. The Jerusalem artichoke treatments in these crops were: total control by regular hand weeding (TOC), mechanical/chemical control (MCC), and no control (NOC). Under the NOC treatment, Jerusalem artichoke infestation at harvest was variable among crops, with the number of shoots ranging from 9 to 25 m−2 in oat and maize stands respectively. The number of Jerusalem artichoke shoots in the MCC plots was reduced by 50 to 99 % in oat and maize, respectively. The highest crop yields in each of the six species were realized under the TOC treatment. Insignificant yield reductions were observed in the NOC treatment of wheat, oat, rape and ryegrass. However, under this management yield reductions of 91 and 81 % occurred in sugarbeet and maize respectively. Depending on the preceding crop, 1–9 shoots m−2 of Jerusalem artichoke were still recorded under the MCC plots in the following season. Consequently, for complete elimination of infestation, volunteers must be controlled in the second and probably in the third year following a Jerusalem artichoke crop. 相似文献
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为了探讨不同检测方法对甜菜ISSR引物扩增效果的影响以及各方法的优缺点,笔者应用非变性聚丙烯酰胺凝胶电泳和琼脂糖凝胶电泳两种检测方法对甜菜ISSR 引物扩增效果进行了检测。利用12个不同的甜菜品种,对10 个ISSR引物进行扩增,分别采用6%的非变性聚丙烯酰胺凝胶电泳、1%的琼脂糖凝胶电泳以及2%的琼脂糖凝胶电泳对ISSR-PCR的扩增产物进行分离。结果表明：6%的聚丙烯酰胺凝胶电泳无论是检测的总条带数还是多态性条带数均远高于琼脂糖凝胶电泳,且条带清晰,易于识别,而2%的琼脂糖凝胶电泳检测的条带数高于或等于1%的琼脂糖凝胶电泳,且条带更易于识别,因此,对于利用ISSR 引物进行QTL定位、指纹图谱构建以及遗传多样性分析时,推荐使用6%的非变性聚丙烯酰胺凝胶电泳,而对于种子纯度鉴定以及需要对ISSR扩增产物进行测序,则推荐使用2%的琼脂糖凝胶电泳。 相似文献
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枳壳基因组DNA提取方法的比较研究 总被引：3，自引：1，他引：2
以枳壳（酸橙）叶片为试验材料,分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA,并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。结果表明,5种方法中SDS法提取的DNA浓度最高（平均为900 ng/ul）,纯度最好（OD260/OD280平均值为1.90）,且SSR-PCR扩增结果与紫外分光光度计和凝胶电泳检测结果基本一致。因此,SDS法是枳壳DNA最佳提取方法,可用于分子检测试验。 相似文献
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大豆不同生长时期基因组DNA提取方法的优化 总被引：3，自引：1，他引：2
以优质大豆品种冀豆7号、冀豆16号、五星1号、五星2号和MK（Maverick）不同生长时期的叶片为材料,分别采用不同方法提取大豆基因组,并通过对提取的DNA的含量测定、琼脂糖凝胶电泳分析和内参基因的PCR扩增,对不同方法所提取的DNA质量进行综合评价。结果表明：在嫩叶期,改进的CTAB法能够 分离出纯度高、质量好的DNA,而在开花期,采用本试验的新改良CTAB法可以有效去除多糖、酚类等物质得到完整性和纯度较好的大豆基因组,为大豆的分子生物学研究奠定了基础。 相似文献
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为了探讨菊芋适应干旱环境的解剖特征,分析菊芋植株营养器官解剖结构与抗旱性的关系,本研究采用石蜡切片和光学显微技术,对2个菊芋品种的根、地上茎、匍匐茎、叶的解剖结构进行了观察比较与参数测定。结果表明:‘青芋1号’与‘青芋2号’的根、地上茎、匍匐茎及叶的解剖结构均存在显著差异。与‘青芋2号’相比,‘青芋1号’根的表皮、皮层厚度分别显著减小35.21%、49.06%,内皮层、韧皮层厚度、韧皮部和木质部所占的比例分别是‘青芋2号’的1.23倍、1.33倍、1.34倍、1.57倍;‘青芋1号’地上茎和匍匐茎表皮的厚度分别是‘青芋2号’的1.18倍和1.38倍;在叶片的解剖结构上,‘青芋1号’的叶片厚度、上表皮、下表皮、栅栏组织厚度分别显著高于‘青芋2号’71.63μm、1.57μm、2.48μm、19.25μm。综合分析说明,‘青芋1号’通过根部表皮、皮层厚度的减小,内皮层、韧皮部的增厚及韧皮部和木质部所占比例的增加来响应干旱胁迫;通过地上茎和匍匐茎表皮厚度的增加,抵御水分流失;通过叶片、上表皮、下表皮的增厚及其内部栅栏组织的增多,利于水分的储存,从而适应干旱环境。本研究结果为菊芋抗旱性品种的鉴定提供解剖学依据。 相似文献
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257份菊芋种质资源表型性状的遗传多样性 总被引：1，自引：0，他引：1
Meng-Liang ZHAO Li-Hui WANG Yan-Jing REN Xue-Mei SUN Zhi-Qiang HOU Shi-Peng YANG Li LI Qi-Wen ZHONG 《作物学报》2020,46(5):712-724
为有效利用我国菊芋种质资源的遗传多样性,分析了257份菊芋种质资源,表明12个数量性状的变异系数(CV)在6%～50%之间,平均为24.75%,单株块茎重的变异系数最大(50%),生育期的变异系数最小(6%),多样性指数(H')分布在1.24～1.53之间,平均为1.44,单株块茎数的多样性指数最高(1.53),叶宽的多样性指数最低(1.24);8个质量性状的多样性指数在0.85～1.08之间,平均为0.98,以块茎习性最大,块茎整齐度最小,大部分性状表现出丰富的遗传多样性; 257份菊芋资源的隶属函数均值介于0.12～0.58之间,其中JA1095材料最高(0.58),其花数和单株块茎重具有明显优势。菊芋资源12个数量性状的相关性分析表明,茎粗、叶长、花和花盘大小可作为今后选育高产菊芋品种的指导目标性状;主成分分析结果表明,7个主成分因子的累计贡献率达66.794%,其中花数量、单株块茎数量、块茎毛根量、块茎表皮光滑程度4个性状是构成菊芋种植表型差异的主要因素;以20个性状为基础的聚类分析将257份种质材料分为5类,其中,第I类和第II类占总资源量的85%。本研究结果为菊芋种质资源的利用及品种选育等提供了重要参考。 相似文献
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