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1.
水稻种子休眠性是关系到稻米品质和稻种质量的一个重要农艺性状。研究水稻种子休眠性遗传及分子机制对培育具有适度休眠性的优良水稻品种具有重要意义。本研究以籼稻品种9311为受体、普通野生稻为供体的染色体片段置换系群体为材料,在后熟不同时间检测群体种子休眠性,对控制种子休眠性的QTL进行定位分析,共定位到14个QTL,分布在第3、第4、第5、第6、第7、第10、第11、第12染色体上。筛选休眠性显著强于背景亲本9311的家系,分析这些家系携带的QTL数目,表明携带的位点越多,休眠性越强。进一步利用家系Q14与9311的F2群体验证了第7染色体标记RM180和RM21323之间存在一个效应较大的QTL qSD-7-2,该位点LOD值为18.49,可解释的表型变异率为33.53%,表明该位点是一个控制普通野生稻种子休眠性的主效QTL,且能稳定遗传。本研究为野生稻种子休眠基因的精细定位及克隆奠定了基础,且为培育强休眠性籼稻品种提供了育种材料。 相似文献
2.
Genetic analysis and QTL mapping of oil content and seed index using two recombinant inbred lines and two backcross populations in Upland cotton 
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下载免费PDF全文 Cottonseed is one of the main by‐products of cotton. To explore the genetic composition of oil content (OC) and seed index (SI) is helpful for utilizing the cottonseed. Under multiple environmental conditions, the genetic structures of OC and SI were explored using two recombinant inbred lines (RILs) and corresponding backcross (BC) populations in Upland cotton. Twenty‐four and 31 quantitative trait loci (QTLs) for OC and SI, respectively, were detected using composite interval mapping, in which 9 QTLs for OC and 18 QTLs controlling SI were simultaneously identified in more than two environments or two populations. Forty‐seven and 37 QTLs with main effects (M‐QTLs) for OC and SI and 114 and 74 QTLs involved in digenic interactions (E‐QTLs), respectively, identified by inclusive composite interval mapping. On average, the E‐QTLs explained a larger portion of the phenotypic variation than the M‐QTLs did. It was concluded that additive effects of single‐locus and epistasis derived from complementary loci with few detectable single‐locus effects played an important role in oil content and seed index in Upland cotton. 相似文献
3.
不同生长环境下水稻结实率数量性状位点的检测 总被引：4，自引：0，他引：4
以籼稻密阳23与粳稻吉冷1号配制所获得的F2:3群体200个家系作为作图群体，在北京、昆明、三亚、公主岭和韩国春川等5个点进行水稻结实率的鉴定，并利用SSR标记对水稻结实率数量性状位点进行检测。结果表明，水稻结实率表型值及其在F3家系群中的分布以及所检测到的QTL数目因生长环境不同而有较大差异，说明QTL与环境有明显的互作效应。水稻结实率在F3家系群中呈接近正态或偏态的连续分布，是多个基因所控制的数量性状。共检测到与水稻结实率相关的QTL 14个，分布于第1、2、3、4、6、7、8、10和12染色体上，对表型变异的贡献率为4.9%～15.3%。分别位于第1、2、6和12染色体RM1~RM259、RM263~RM6、RM340~RM30、RM270~RM17区间的qSSR1、qSSR2、qSSR6和qSSR12至少在2种生长环境下均检测到，对表型变异的贡献率分别为4.9%～8.4%、4.8%～7.2%、7.6%～10.7%和7.4%～10.4%。以上多数QTL增效等位基因均来自吉冷1号，基因作用方式主要为部分显性或显性或超显性。 相似文献
4.
Y. Xue S. Q. Zhang Q. H. Yao R. H. Peng A. S. Xiong X. Li W. M. Zhu Y. Y. Zhu D. S. Zha 《Euphytica》2008,164(3):739-744
In the present study, quantitative trait loci (QTLs) controlling seed storability based on relative germination rate (%) were
dissected using a saturated linkage map and a recombinant inbred lines (RILs) derived from a cross of japonica cultivar Asominori (Oryza sativa L.) and indica cultivar IR24 (Oryza sativa L.). A total of three QTLs (qRGR-1, qRGR-3 and qRGR-9) were detected on chromosomes 1, 3 and 9 with LOD score ranging from 3.45 to 6.95 and the phenotypic variance explained from
16.72% to 28.63%. The IR24 alleles were all associated with seed storability at all the three QTLs. The existence of these
QTLs was confirmed using IR24 chromosome segment substitution lines (CSSLs) in Asominori genetic background (AIS). By QTL
comparative analysis, the QTL, qRGR-9 on chromosomes 9 appeared to be consistent with another rice population, this region may provide an important region for
isolating this responsible gene. These results also provide the possibilities of enhancing Seed storability in rice breeding
program by marker-assisted selection (MAS) and pyramiding QTLs.
Y. Xue and S. Q. Zhang—joint first authors. 相似文献
5.
B. Fofana D. G. Humphreys G. Rasul S. Cloutier A. Brûlé-Babel S. Woods O. M. Lukow D. J. Somers 《Euphytica》2009,165(3):509-521
Hard white wheat (Triticum aestivum L.) is a value-added product because of its processing advantages over red wheat; however, white wheat tends to be more susceptible
to pre-harvest sprouting (PHS). To identify quantitative trait loci (QTLs) associated with PHS tolerance, we developed a doubled
haploid (DH) mapping population from the cross AC Domain (red seeded) × White-RL4137 (white seeded). A genetic map was constructed
using microsatellite markers located on chromosome groups 3, 4, 5 and 6. A population of 174 DH lines was characterized for
important aspects of PHS including sprouting index, germination index, Hagberg falling number and seed coat colour. A total
of 11 QTLs were identified on group 3 chromosomes and on chromosome 5D. Seven QTLs associated with the PHS traits were found
to be co-incident with seed coat colour on chromosomes 3A, 3B and 3D. The 5D PHS QTL was notable because it is independent
of seed coat colour. 相似文献
6.
Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.) 总被引：13，自引：0，他引：13
Summary Grain yield in the maize (Zea mays L) plant is sensitive to drought in the period three weeks either side of flowering. Maize is well-adapted to the use of restriction fragment length polymorphisms (RFLPs) to identify a tight linkage between gene(s) controlling the quantitative trait and a molecular marker. We have determined the chromosomal locations of quantitative trait loci (QTLs) affecting grain yield under drought, anthesis-silking interval, and number of ears per plant. The F3 families derived from the cross SD34(tolerant) × SD35 (intolerant) were evaluated for these traits in a two replicated experiment. RFLP analysis of the maize genome included non-radioactive DNA-DNA hybridization detection using chemiluminescence. To identify QTLs underlying tolerance to drought, the mean phenotypic performances of F3 families were compared based on genotypic classification at each of 70 RFLP marker loci. The genetic linkage map assembled from these markers was in good agreement with previously published maps. The phenotypic correlations between yield and other traits were highly significant. In the combined analyses, genomic regions significantly affecting tolerance to drought were found on chromosomes 1,3,5,6, and 8. For yield, a total of 50% of the phenotypic variance could be explained by five putative QTLs. Different types of gene action were found for the putative QTLs for the three traits. 相似文献
7.
8.
A. Blanco C. de Giovanni B. Laddomada A. Sciancalepore R. Simeone K. M. Devos M. D. Gale 《Plant Breeding》1996,115(5):310-316
Seed storage protein content of durum wheat (Triticum turgidum var. durum) has an important effect on nutritional value and pasta-making characteristics. The objective of this study was to determine by association with genetic markers the number, chromosomal location, and magnitude of effect of quantitative trait loci (QTLs) controlling protein concentration in kernels. A set of 65 recombinant inbred lines (RIs) was developed by single seed descent from a cross between cultivated durum wheat cv. ‘Messapia’ (low protein content) and accession MG4343 of the wild tetraploid wheat var. dicoccoides (high protein content). This population was characterized for eight morphological, six storage protein, one isozyme and 124 RFLP loci. Field trials were conducted in one location in 1993 and two locations in 1994. QTLs were mapped by regression analysis on each marker locus for each location and for the average across environments. A total of six putative QTLs were located on chromosome arms 4BS, SAL, 6AS, 6BS and 7BS. The number and size of QTLs detected varied across environments. The marker with the highest r2 value per QTL in each environment and across environments was chosen for a multiple linear regression analysis, which explained 49.2- 56.4% of the phenotypic variation for protein content. Only some of the markers were found to be negatively associated with plant grain yield and/or seed weight in one or two of the environments. 相似文献
9.
水稻种子耐贮藏性QTL主效应和上位性效应分析 总被引：2，自引：0，他引：2
利用珍汕97B／密阳46构建的RIL群体及其相应分子遗传图谱,采用种子加速老化鉴定法,以处理后的相对发芽率(％)作为该材料耐贮藏性的考察指标,分别对老化处理7d和14d后测得的数据进行QTL定位和上位性分析。共检测到2个主效应QTL与控制种子耐贮藏性有关。在老化程度相对较轻时,qSS9-1起忍耐作用：而在老化程度相对较重时,则有qSS4基因在起作用。试验还检测到5对耐贮藏性的上位性互作OTL,其贡献率为8．14％～13．51％,涉及第1、2、3、4、5、6、9、12等8条染色体。其中在7d老化处理试验中,检测到2对效应值相对较小的上位性互作;而在14d老化处理试验中,检测到3对效应值相对较大的上位性互作。 相似文献
10.
以东乡普通野生稻和日本晴为亲本构建的染色体片段置换系为研究材料,2019年分别在北京、山东临沂和江西南昌对分蘖数、穗粒数和粒形等11个产量相关性状进行多环境鉴定,结合染色体片段置换系基因型数据定位水稻产量相关性状QTL。3个环境共检测到68个QTL,包括株高4个、穗长5个、分蘖数2个、一次枝梗数7个、一次枝梗粒数8个、二次枝梗数8个、二次枝梗粒数10个、每穗粒数6个、千粒重7个、粒长8个和粒宽3个;LOD值介于2.50~12.66之间,贡献率变幅为4.67%~27.79%,15个QTL的贡献率大于15%;24个QTL与已报道位点/基因位置重叠,44个QTL为新发现位点;6个QTL在2个环境能被检测到,1个QTL qTGW2能在3个环境检测到,且是还未报道的新位点。最后,利用BSA法验证了qPH7、qPBPP8-2和qGW10三个QTL的可靠性。本研究将为后续产量相关性状基因克隆以及进一步解析其遗传基础和分子调控机制奠定基础。 相似文献
11.
冷水胁迫下水稻幼苗期根系性状的QTL分析 总被引：4，自引：1，他引：4
本研究以籼粳交“密阳23/吉冷1号”的F2∶3 代200个家系作为作图群体，在自然和12℃冷水胁迫下，进行水稻幼苗期根系性状的鉴定，并以利用SSR标记构建的分子连锁图谱为基础，对水稻幼苗期的根数、最大的根长、最大根的根径、根干重、根/苗比等根系性状进行了数量性状位点（QTLs）分析。结果表明，上述根系性状在F3代家系群均表现为连续分布，认为是由多基因所控制的数量性状。冷水胁迫下，在第1、2、6、11和12染色体上共检测到与根系性状相关的QTL 17个，对表型变异的解释率为5.8%～15.2%，其中与最大根的根径相关，位于第2染色体RM263-RM6区间的qCRD2和位于第11染色体RM21-RM206区间的qCRD11，以及与根干重相关，位于第2染色体RM262-RM263区间的qCRWT2和位于第11染色体RM229-RM21区间的qCRWT11贡献率较大，分别为15.0%、15.2%、10.6%和12.2%。这些基因的作用方式为部分显性或显性或超显性。 相似文献
12.
Identification of QTLs for rice grain size and shape of Iranian cultivars using SSR markers 总被引：6，自引：0，他引：6
Grain size is a main component of rice appearance quality. In this study, we performed the SSR mapping of quantitative trait loci (QTLs) controlling grain size (grain length and breadth) and shape (length/breadth ratio) using an F2 population of a cross between two Iranian cultivars, Domsephid and Gerdeh, comprising of 192 individuals. A linkage map with 88 markers was constructed, which covered 1367.9 cM of the rice genome with an average distance of 18 cM between markers. Interval mapping procedure was used to identify the QTLs controlling three grain traits, and QTLs detected were further confirmed using composite interval mapping. A total of 11 intervals carrying 18 QTLs for three traits were identifed, that included five QTLs for grain length, seven QTLs for grain breadth, and six QTLs for grain shape. A major QTL for grain length was detected on chromosome 3, that explained 19.3% of the phenotypic variation. Two major QTLs for grain breadth were mapped on chromosomes 3 and 8, which explained 34.1% and 20% of the phenotypic variation, respectively. Another two major QTLs were identified for grain shape on chromosomes 3 and 8, which accounted for 27.1% and 20.5% of the phenotypic variance, respectively. The two QTLs that were mapped for grain shape coincided with the major QTLs detected for grain length and grain breadth. Intrestingly, gs2 QTL specific to grain shape was detected on chromosome 2 that explained 15% of the phenotypic variation. 相似文献
13.
多环境下稻米粒重的QTL定位 总被引：2，自引：0，他引：2
以粳稻Asominori为遗传背景的染色体片段置换系（CSSLs）群体为材料,利用基于性状-标记多元回归分析方法对稻谷粒重和精米粒重进行多环境的QTL定位。结果在5个环境共检测到6个粒重相关QTL,分布于第1、6、7和8染色体上,对表型变异的贡献率介于13%~35%;其中控制精米粒重的qMRW-1a和稻谷粒重的qPRW-1在不同环境中均能稳定表达,且均位于第1染色体RFLP标记XNpb113附近,该基因座还同时控制着粒宽。qMRW-1a和qPRW-1共同对应的置换系AIS8和AIS11与Asominori 的粒重差异在不同环境中均显著(P < 0.05),表明该QTL的等位基因在不同环境中效应显著。比较发现该QTL在不同遗传群体中均能被重复检测到,且与蔗糖磷酸合酶基因（SPS）位置一致,推测该QTL与淀粉合成代谢有关。qMRW-1a 和qPRW-1在不同环境条件和遗传背景中表达,因此可用于进一步的精细定位研究。 相似文献
14.
利用DH群体动态检测水稻抗褐飞虱数量性状基因位点 总被引：9，自引：0，他引：9
利用籼粳交珍汕97／武育粳2号F1花培获得的190个双单倍体群体(doubled—haploid population,DH系)及其构建的179个SSR分子标记遗传图谱,通过对DH系群体苗期重复接虫试验和2个不同时期对褐飞虱危害程度进行动态调查,并应用Mapmaker／exp Version3．0和Windows QTL Cartographer V2．0对水稻抗褐飞虱数量性状基因位点(quantative trait locus,QTL)进行动态检测和遗传效应分析。结果表明,在苗期对褐飞虱抗性的检测中,共检测到6个抗性QTL,分别位于第2、3、4、8和10染色体上,各QTL的LOD值分别为2．22-4．64,贡献率为5．04％～13．73％,第3染色体和第4染色体上各有1个OTL的加性效应为正值,表明来自于亲本武育粳2号的这2个位点的等位基因可以提高水稻对褐飞虱的抗性,其余4个QTL的加性效应均为负值,表明来自于亲本珍汕97的这些位点的等位基因可以提高水稻对褐飞虱的抗性。 相似文献
15.
棉花叶绿素含量和光合速率的QTL定位 总被引：10，自引：2，他引：8
为了探讨棉花光合作用及相关生理性状的遗传规律, 利用四交分离作图群体泗棉3号/苏12//中4133/8891的273个F2：3家系为材料,用MAPQTL5.0软件及区间作图方法(IM), 对棉花叶绿素含量及光合速率进行了QTL分析。检测到3个与叶绿素含量相关的QTL, 分别位于染色体D6、D8和A10, 解释性状表型变异的4.3%, 4.5% 和5.2%。检测到3个与光合速率相关的QTL, 位于D5、D6和A11染色体, 解释性状表型变异的3.8%,7.4% 和8.4%。两个性状所有QTL的遗传效应均以加性效应为主。本研究定位的棉花叶绿素含量和光合速率QTL均是首次报道,可尝试应用于高光效育种的分子标记辅助选择。 相似文献
16.
以普通小麦品种川农16与人工合成小麦衍生品种川麦42为亲本构建了含有127个株系的重组自交系(RIL)群体,将2008和2009年收获的种子用玉米象(Sitophilus zeamais)虫卵进行接种,并于2009年11月开始储藏,次年8月和10月进行虫害调查。利用复合区间作图法(CIM)对RIL群体的抗虫性进行QTL定位分析。利用2008年收获群体检测到位于3B、2D和3D染色体的3个QTL,贡献率依次为10.2%、8.5%和8.3%;利用2009年收获群体检测到位于3D和4B的2个QTL,贡献率分别为8.5%和11.3%。位于3D染色体Xbarc6–Xgwm112标记区间内的QTL在年度间重复检测到,贡献率为8.3%~8.5%,抗性位点来自川农16。 相似文献
17.
稻米直链淀粉含量基因座位的分子标记定位 总被引：29，自引：0，他引：29
以直链淀粉含量(AC)中等的CT9993和泰国优质的香软米KDML105杂交产生的152个重组近交系 (RIL)为材料, 构建了含83个RFLP、 69个AFLP和15个微卫星(SSLP)标记的分子标记连锁图 , 标记间平均距离为12.98 cM。 应用该连锁图对控制稻米AC的基因座位(QTL)进行了分析 。 结果表明： 稻米AC主要受两个主效QTL和5个微效QTL的共同控制 相似文献
18.
Leonardo Corrêa da Silva Thiago Lívio Pessoa Oliveira de Souza Cosme Damião Cruz Pedro Crescêncio Souza Carneiro Fabyano Fonseca e Silva Everaldo Gonçalves de Barros Rosana Pereira Vianello Carlos Eduardo Lazarini da Fonseca Qijian Song Perry B. Cregan José Eustáquio de Souza Carneiro 《Euphytica》2018,214(12):221
This paper proposes the construction of a genetic linkage map with 376 recombinant inbred lines (RILs) derived from a cross between Mesoamerican?×?Andean common bean (Phaseolus vulgaris L) parents based on single nucleotide polymorphism (SNP) markers; and to detect quantitative trait loci (QTLs) associated with seven morpho-agronomic traits: number of days to flowering (DF), number of days to maturity (DM) or crop cycle; plant architecture (ARC); seed yield (YLD); degree of seed flatness (SF); seed shape (SS); and 100-seed weight (SW). A total of 3060 polymorphic SNP markers were used and 2041 segregated at a 1:1 ratio in the RIL population, as expected. These markers were subjected to linkage analysis in each chromosome. The genetic linkage analysis resulted in linkage maps with a total of 1962 markers spanning 1079.21 cM. A total of 29 QTLs associated with seven morpho-agronomic traits were detected on the 11 chromosomes, which explained between 3.83 and 32.92% of the phenotypic variation in DF. A total of 18 candidate genes associated with the detected QTLs were identified and related with biological processes, molecular functions and cellular components. 相似文献
19.
Qun Wan Zhengsheng Zhang Meichun Hu Li Chen Dajun Liu Xiao Chen Wei Wang Jing Zheng 《Euphytica》2007,158(1-2):241-247
A genetic linkage map of chromosome 6 was constructed by using 270 recombinant inbred lines originated from an upland cotton
cross (Yumian 1 × T586) F2 population. The genetic map included one morphological (T1) and 18 SSR loci, covering 96.2 cM with an average distance of 5.34 cM between two markers. Based on composite interval mapping
(CIM), QTL(s) affecting lint percentage, fiber length, fiber length uniformity, fiber strength and spiny bollworm resistance
(Earias spp.) were identified in the t1 locus region on chromosome 6. The allele(s) originating from T586 of QTLs controlling lint percentage increased the trait
phenotypic value while the alleles originating from Yumian 1 of QTLs affecting fiber length, fiber length uniformity, fiber
strength and spiny bollworm resistance increased the trait phenotypic value. 相似文献
20.
Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L.) 总被引：1，自引：0，他引：1
Zheng-Sheng Zhang Yue-Hua Xiao Ming Luo Xian-Bi Li Xiao-Ying Luo Lei Hou De-Mou Li Yan Pei 《Euphytica》2005,144(1-2):91-99
A genetic linkage map with 70 loci (55 SSR, 12 AFLP and 3 morphological loci) was constructed using 117 F2 plants obtained from a cross between two upland cotton cultivars Yumian 1 and T586, which have relatively high levels of
DNA marker polymorphism and differ remarkably in fiber-related traits. The linkage map comprised of 20 linkage groups, covering
525 cM with an average distance of 7.5 cM between two markers, or approximately 11.8% of the recombination length of the cotton
genome. The present genetic linkage map was used to identify and map the quantitative trait loci (QTLs) affecting lint percentage
and fiber quality traits in 117 F2:3 family lines. Sixteen QTLs for lint percentage and fiber quality traits were identified in six linkage groups by multiple
interval mapping: four QTLs for lint percentage, two QTLs for fiber 2.5% span length, three QTLs for fiber length uniformity,
three QTLs for fiber strength, two QTLs for fiber elongation and two QTLs for micronaire reading. The QTL controlling fiber-related
traits were mainly additive, and meanwhile including dominant and overdominant. Several QTLs affecting different fiber-related
traits were detected within the same chromosome region, suggesting that genes controlling fiber traits may be linked or the
result of pleiotropy. 相似文献