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1.
YU Hao SONG Yuefen LI Li LIU Di 《东北农业大学学报(英文版)》2007,14(1):27-30
Glutamate decarboxylase (GAD) has been found in animal and higher plant tissues as well as in yeasts and microorganisms. In animals the enzyme plays an important role in central nervous system activity because the enzyme substrate glutamic acid is a mediator of excitation process and the product, gamma-aminobutyric acid, is the most important mediator of inhibition process in the central nervous system. GAD65 is one form of the glutamate decarboxylases (GAD), GAD65 has been identified as a major autoantigen in type 1 diabetes, so the GAD65 gene of porcine was cloned by RT-PCR method to construct phylogenetic tree, the homology of 13 glutamate decarboxylases (GAD) of different origin was analyzed by multiple alignment. 相似文献
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In the case of recently established populations of Solenopsis invicta in Taiwan,analysis of genetic variation may provide various dimensions regarding the historical demographic events of this invasive species,which represents a novel opportunity to study the genetic consequences of invasiveness over an ex- tremely short time period.Here we describe genetic structure of two introduced S.invicta populations, Taoyuan and Chiayi,in Taiwan using two classes of markers,nuclear(microsatellites)and mitochondrial (mtDNA)genomes.Pronounced regional differentiation inferred from either AMOVA or Bayesian clustering approach at both genomes suggests that the two populations most likely originate from separate introductions. Furthermore,significant mtDNA but not nuclear differentiation between sympatric social forms suggests two scenarios that interform gene flow is mainly mediated by males,and queens of each form commonly fail to become egg-layers of alternate form.Isolation by distance(IBD)obtained from microsatellites is absent in monogynes,indicating sexuals of this form retain the superior dispersal ability to homogenize the nuclear sig- nature among spatially isolated areas;however,lack of IBD in less vagile polygyne may result from frequent human mediated jump dispersal that erodes the geographical restrictions of genetic exchange.The patterns observed here not only provide insights into how social organization influences the interform gene flow but also reinforce different breeding strategies pursued by two forms in the character of shaping the genetic varia- tion at two levels of genomes. 相似文献
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ZHOU Zhong-kai ;HUA Ze-tian ;YANG Yan ;ZHENG Pai-yun ;ZHANG Yan ;CHEN Xiao-shan 《中国农业科学(英文版)》2014,(5):1146-1153
In order to understand the effect of starch molecular characteristics on the gel structure, which subsequently influence the gel digestion behaviours, three wheat starches, control （conventional wheat starch）, two new wheat cultivars with different genetic backgrounds （by knocking out SBE IIb and SBE IIa, respectively） were used in this study. In comparison with control, slight differences in the morphology of the starch granules of new wheat 1 were observed, whereas the starch granules of new wheat 2 had irregular shapes both for A-type granules and B-type granules. Starch molecular weight size was determined by SE-HPLC, and the results indicate that there was a subtle increase in the amylose content in the starch of new wheat 1 compared to that of control. The starch of new wheat 2 had the highest amylose content, and the molecular weight （MW） of its amylopectin was the lowest among the three starches. Fourier transform infrared spectroscopy （FTIR） was employed to investigate starch gel structure and the results suggest that the molecules of starch gel from new wheat 2 are more likely to re-associate to form an organized conformation. The digestion behaviours of the three starch gels were measured using a mixture of pancreatin a-amylase and amyloglucosidase. The results indicated that the starch gels of control and new wheat 1 had very high digestibility of 91.7 and 91.9%, respectively, whereas the digestibility of wheat 2 starch gel was only 36.2%. In comparison with the digestion curve pattems of control and new wheat 1 starch gels, the new wheat 2 exhibited a much lower initial velocity. These results indicated that the molecules in the starch of new wheat 2 are more readily to re-associate to form an organized structure during gel formation because of its unique molecular characteristics. 相似文献
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ZHAO Hong-yan LI Jie LIU Jing-jing Lü Yu-cai WANG Xiao-fen CUI Zong-jun 《中国农业科学(英文版)》2013,(6):1087-1097
This study evaluated the microbial community dynamics and maturation time of two compost systems: biogas slurry compost and cow manure compost, with the aim of evaluating the potential utility of a biogas slurry compost system. Denaturing gradient gel electrophoresis (DGGE), gene clone library, temperature, C/N ratio, and the germination index were employed for the investigation, cow manure compost was used as the control. Results showed that the basic strip and dominant strips of the DGGE bands for biogas slurry compost were similar to those of cow manure compost, but the brightness of the respective strips for each system were different. Shannon-Weaver indices of the two compost systems differed, possessing only 22% similarity in the primary and maturity stages of the compost process. Using bacterial 16S rRNA gene clone library analysis, 88 bacterial clones were detected. Further, 18 and 13 operational taxonomic units (OTUs) were present in biogas slurry and cow manure compost, respectively. The 18 OTUs of the biogas slurry compost belonged to nine bacterial genera, of which the dominant strains were Bacillus sp. and Carnobacterium sp.; the 13 OTUs of the cow manure compost belonged to eight bacterial genera, of which the dominant strains were Psychrobacter sp., Pseudomonas sp., and Clostridium sp. Results demonstrated that the duration of the thermophilic phase (more than 50°C) for biogas slurry compost was 8 d less than the according duration for cow manure compost, and the maturation times for biogas slurry and cow manure compost were 45 and 60 d, respectively. It is an effective biogas slurry assimilate technology by application of biogas slurry as nitrogen additives in the manufacture of organic fertilizer. 相似文献
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LIU Kai-qi XIANG Mei-mei LIU Ren ZENG Yong-san YANG Yong YU Jin-feng JIANG Xin-yin ZHANG Yue-li 《浙江大学学报(农业与生命科学版)》2004,30(4):429-429
The pectin is a backbone of the plant cell wall, its network structure will systemicly resolve when the plant cell splits up and forms. The pectinase produced by Rhizoctonia mainly acts on the pectin of cell wall, and causes the maceration of tissue and the death of protoplast. Polygalacturonase (PG) can decompose the galacturonic acid of disease tissue. The research defined the PG activities of extracellular metabolite of the different virulence Rhizoctonia isolates, and testifid the e… 相似文献
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JinYi 《东北农业大学学报(英文版)》1995,2(1):66-74
The analysis method of the triple test cross design has been discussed carefully form the two factor experiment design and the genetic models of additive-dominant effect and of epistasis effect-Two points different from the previous reprots have been concluded:(1)both the degrees of freedom of the orthogonal terms C2 and C3 are m;(2) the denominator in the F test to C2 and C3 is the error mean of square between plots. 相似文献
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Balanced development between industrial and employment structures are important indicators of regional economic and social health. By introducing indicators such as industrial structure deviation, comparative labor productivity, international comparison of relative labor productivity as well as employment elasticity, the paper further probed into intricate causes for the imbalance between industrial and employment structures in this specific region from a new angle which based on the evolution process analyses of both industrial and employment structures in Liaoning, Jilin and Heilongjiang in Northeast China. The paper also suggested policy measures to strengthen agricultural developments and to facilitate the balance between industrial and employment structures. 相似文献
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3个杨桃品种茎次生木质部导管分子结构研究(英文) 总被引：1，自引：0，他引：1
[Objective] The aim of this study was to provide the theoretical basis for variety identification of carambola and pomiculture.[Method] The molecular structure of vessels in stem secondary xylem from three carambola varieties of Malaysia 8,Misi and Malaysia B17 were studied by cell image analysis system and microphotography.[Result] The molecular structure of vessels in stem secondary xylem from carambola was as follows:the first type had tails in two ends,the second type had tail in one end,and the third type had no tails in two ends.The average length of vessel elements was from 328.88 to 366.09 μm,while its average width was from 44.61 to 52.43 μm.Most of the end wall was simple perforation plates,while the alternate-opposite pitting was the major forms of pitting.[Conclusion] Most characteristics of vessel elements from carambola were evolutionary characters of vessel elements in the process of phylogeny,but there were more primitive characters,for example,both two ends of vessel had tails or just one end had tail,and end wall was inclined.The molecular structure of vessel from carambola was in accordance with its ecological adaptability,and the growth characteristics of different varieties were also in accordance with the difference of molecular structure of vessel. 相似文献
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《（《农业科学与技术》）编辑部》2008,(4)
[Objective] The aim of this study was to provide the theoretical basis for variety identification of carambola and pomiculture.[Method] The molecular structure of vessels in stem secondary xylem from three carambola varieties of Malaysia 8,Misi and Malaysia B17 were studied by cell image analysis system and microphotography.[Result] The molecular structure of vessels in stem secondary xylem from carambola was as follows:the first type had tails in two ends,the second type had tail in one end,and the third type had no tails in two ends.The average length of vessel elements was from 328.88 to 366.09 μm,while its average width was from 44.61 to 52.43 μm.Most of the end wall was simple perforation plates,while the alternate-opposite pitting was the major forms of pitting.[Conclusion] Most characteristics of vessel elements from carambola were evolutionary characters of vessel elements in the process of phylogeny,but there were more primitive characters,for example,both two ends of vessel had tails or just one end had tail,and end wall was inclined.The molecular structure of vessel from carambola was in accordance with its ecological adaptability,and the growth characteristics of different varieties were also in accordance with the difference of molecular structure of vessel. 相似文献
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《河北农业大学学报》创刊年代考 总被引：3，自引：0，他引：3
清光绪二十八年(1902)河北农业大学前身—直隶农务学堂诞生,经几易其名,于1958年更名为河北农业大学至今。清光绪三十一年(1905)直隶高等农业学堂时期创办了《北直农话报》,清光绪三十四年(1908)更名为《直隶农务官报》,中华民国七年(1918)改出《农学月刊》,中华民国十七年(1928)易名为《河大农刊》,中华民国二十三年(1934)更名为《河北通俗农刊》,中华民国二十四年(1935)易名为《河北农林学刊》,1948年更名为《河北农学院研究专刊》,1959年更名为《河北农业大学学报》至今。《河北农业大学学报》前身诸刊都与现时的《河北农业大学学报》有着一脉相承的历史渊源,各刊之间联系紧密,连续性、继承性强。因此,《河北农业大学学报》的创刊时间应追溯至1905年创办的《北直农话报》。 相似文献
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生态旅游潜在客源市场特征的调查研究--以湖南永州金洞森林旅游开发为例 总被引：5，自引：2，他引：3
李慧云 《湖南农业大学学报(自然科学版)》2004,30(2):165-168
为探明客源市场生态旅游消费的潜在特征,采用问卷调查的形式,就长沙市居民对湖南金洞生态旅游开发的意向等问题进行抽样调查．结果显示,生态旅游符合人们“回归自然”的旅游新时尚,有着极大的开发空间,指出生态旅游的开发要注重环境保护和可持续发展．开发的产品要以休闲度假类的大众产品为主,开发生态旅游都市客源市场还要多种渠道并用,尤其是要注重媒体的宣传． 相似文献
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任琪 《安徽农业大学学报》2008,(3):132-134
国家贫困生资助政策实施以来,对贫困生帮助很大,同时在实际运行中还存在着一些问题。本文提出贫困生认定工作仍需要进一步采取各种相关配套措施,以推动和保障贫困生资助工作更好地开展。 相似文献
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应用萄聚糖凝胶柱层析对水牛梭形住肉孢子虫包囊包溶性抗原进行了纯化。结果表明：纯化水牛梭形住肉孢子虫包囊抗原的最适条件为：选用萄聚糖凝胶Ｇ—１００柱层析系统；洗脱液为０３ＭＰＢＳ（ｐＨ７２），床体积为１０ｃｍ×１６５ｃｍ，上样体积为５００ＨＬ；流速为１２ｍＬ／ｈ。纯化抗原可使琼脂凝胶双扩散试验的阳性检出率提高３０％。 相似文献
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[目的]通过体外抗氧化体系比较黑豆不同部分馏油抗氧化性活性。[方法]通过正交试验优化索氏提取黑豆馏油的最佳工艺,提取黑豆不同部分馏油;研究黑豆不同部分馏油对羟基自由基(·OH)、DPPH自由基(DPPH·)的清除能力。[结果]在样品浓度为1.0 mg/mL时,黑豆不同部分馏油对·OH的清除率分别为全豆馏油83.9%、豆黄(黑豆去皮部分)馏油64.8%、豆皮馏油17.3%,对DPPH·的清除率分别为全豆馏油41.3%、豆黄馏油33.2%、豆皮馏油77.9%。[结论]黑豆不同部分馏油均具有较好的抗氧化性,是良好的天然抗氧化剂。黑豆不同部分馏油对·OH的清除能力从大到小依次为全豆馏油、豆黄馏油、豆皮馏油,对DPPH·的清除能力从大到小依次为豆皮馏油、全豆馏油、豆黄馏油。 相似文献
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为了预测厚胶合板弹性模量,通过简化层合板理论,该文建立了胶合板弹性模量预测模型,并以单板条、经过涂胶热压处理的单板条和相同工艺条件下的单板层积材的弹性模量,采用4种不同铺层方式的19层桉树胶合板对模型进行了验证。结果表明:3种预测值与实测值的趋势一致,相关系数R2顺纹在0.86以上,横纹在0.88以上,但是精度不同。采用单板条弹性模量预测的胶合板弹性模量比实测值偏低;采用经过涂胶热压处理后的单板条弹性模量预测的胶合板弹性模量比实测值偏高;采用相同工艺条件下的单板层积材弹性模量预测的胶合板弹性模量与实测值偏差较小,顺纹平均误差为4.64%,横纹平均误差10.94%。因此,采用相同工艺条件下的单板层积材的弹性模量来预测胶合板的弹性模量是可行的。 相似文献
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分析了入世4年来,我国水果出口在量上实现了突破,但并没有实现质变的主要原因,指出我国水果业存在的问题。论述了引进外资对发展我国水果业的意义,并提出了具体可行的对策与建议。 相似文献
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动物疫病是畜牧业生产的大敌,要发展畜牧业生产就要防治动物疫病。但在疫苗接种时,经常出现正常反应外的其他不利于机体的反应,如废食、皮疹、休克、死亡等,正确处理或避开这些问题,对推行动物疫病的计划免疫,实施强制免疫,保护人畜安全具有十分重要的现实意义和作用。 相似文献