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1.
The growth dynamics of root system and aboveground of the grafted seedlings of different Camellia oleifera cultivars are studied by skeleton method and root scanning. The results of 8-months’ observation on grafted seedlings of 5 C. oleifera cultivars showed that: the growth of aerial parts and root of the C. oleifera seedlings were not entirely in sync, during the time of low temperature from November to February, the root system still in sustained growth though the aerial parts of the 5 cultivars grew slowly. Remarkable differences were existed in root length, root surface area, root volume, root biomass and other indicators after 11-months’ growth. The mean value of total length of root-system was up to 243.3 cm and 143.4 cm for the minimum, the mean value of total root surface area was up to 50.2 cm2 and 31.7 cm2 for the minimum, the mean value of root volume of the C. oleifera seedlings was up to 0.843 cm3 and 0.578 cm3 for the minimum which lived 11 months after grafting. All the root length, root surface area, root volume of the 5 cultivars have remarkable differences. 相似文献
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In order to set up the genetic diversity system of Eucommia ulmoides Oliver based on SSR molecular markers, the establishment of SSR-PCR reaction system and screening out SSR marker primer showing high polymorphism were studied. A L9(34) orthogonal design was performed to optimize the main factors of the SSR-PCR reaction system. The results indicated that the best SSR-PCR reaction system for E.ulmoides was DNA template 1 μL (30~60 ng·μL-1), 2×Taq PCR Master Mix 10 μL, primer 1 μL with the total volume of 25 μL. The PCR reaction system had high stability and repeatability, the pairs of SSR primers with high polymorphism were gotten. The 8 E.ulmoides samples' DNA sequence was amplified with 13 pairs of SSR primers by SSR-PCR technique, 34 alleles were detected, 2.6 alleles were detected from per site on average. Each allele's effective number was 1.751 5, and the h value was 0.379 8, the average I value was 0.643 3. This study is helpful in using SSR molecular marker to analyze genetic diversity and genetic relationship in E. ulmoides. 相似文献
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The optimization of the Genomic-SSR reaction system is a basic protocol when the Genomic-SSR is used for genetic analysis in Liriodendron. The concentrations of Mg2+, dNTP, Primer and rTaq were tested by L9(34) orthogonal experiment and single factor gradient experiment to gain the optimal reaction system. The results indicated that the optimal reaction system should contain 75 ng of genomic DNA, 1 μL of 10×buffer, 0.4 μL of 10 mmol·L-1 dNTP, 0.75 μL of 2.5 mmol·L-1 MgCl2, 0.25μL of 10 μmol·L-1 Primer, 0.05 μL of 5 U rTaq and final volume of 10μL. Repeated trials and two verification tests showed that this optimal reaction system was stable, reliable, efficient and suitable for the applications of Genomic-SSR in Liriodendron population genetics and quantitative genetics research. 相似文献
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为建立稳定的红楠ISSR-PCR最佳反应体系,在探索红楠基因组DNA提取的基础上,利用单因子试验和正交试验设计对反应体系进行优化。首先采用单因子试验对Mg2+、引物、模板DNA、dNTPs的不同浓度水平进行优化,找出ISSR-PCR反应各因素的最佳浓度;同时为进一步增加结果的可靠性,采用正交试验设计方法 对Mg2+、引物、模板DNA、dNTPs 4个因素3个浓度水平进行优化和筛选。综合两种试验方法结果,最终获得了红楠ISSR-PCR最佳反应体系:反应体系为20 μL,Taq酶0.05 U·μL-1,Mg2+ 2.0 mmol·L-1,模板DNA 1 ng·L-1,dNTPs 0.3 mmol·L-1,引物(835) 0.5 μmol·L-1,1×PCR缓冲液。建立重复性好、稳定性优良的ISSR-PCR反应体系,为下一步红楠群体遗传结构和遗传变异研究提供了技术支持。 相似文献
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Optimizing SSR-PCR system of Panax ginseng by orthogonal design 总被引：1，自引：0，他引：1
An orthogonal design was used to optimize SSR-PCR amplification system using Panax ginseng genomic DNA as template. Four levels of five factors （DNA template, Taq DNA polymerase, Mg^2＋, primer, and dNTP） and annealing temperature have been tested separately in this system. The results demonstrated the reaction efficiency was affected by these factors. Based on the results, a stable, productive and reproducible PCR system and cycling program for amplifying a ginseng SSR locus were obtained： 20 μL system containing 1.0 U Taq DNA polymerase, 2.0 mmol·L^-1 Mg^2＋, 0.2 mmol·L^-1 dNTPs, 0.3 μmol·L^-1 SSR primer, 60 ng· μla^-1 DNA template, performed with a program of 94℃ for 5 min, 94℃ for 30 s, annealing at 56.3℃ for 30 s, 72℃ for 1 min, 37 cycles, finishing at 72℃ for 7 min, and storing at 4℃. 相似文献
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The nutritional components and poisonous elements in flowers of Camellia chekiangoleosa, C. polydonta, C. semiserrata and C. azalea were studied. The results indicated that the soluble sugar content of the four Camellia flowers had no difference. The maximum protein content was 82.9 g·kg-1, detected in the flower of C. semiserrata. And the minimum protein content was 56.8 g·kg-1, found in C. azalea. Vitamin C and β-carotene contents in C. azalea and C. semiserrata were higher, and in C. polydonta and C. chekiangoleosa were lower. The total amino acid contents of the four Camellia were 68.41、67.26、50.77、66.87 g·kg-1 respectively, the essential amino acid contents were 33.86、32.70、27.21、33.79 g·kg-1 respectively, and the proportion of essential amino acid in total amino acid of the four Camellia were 49.50%, 48.62%, 53.59% and 50.53% respectively. The contents of Ca, Mn and Cu in C. chekiangoleosa were the highest. Zn and Se contents were the maximum and Ca was the minimum in C. polydonta. The contents of Fe and Se were the lowest in C. semiserrata. Fe content were the maximum and Mn, Zn and Cu the minimum in C. azalea. The trace element contents in the flower of the four Camellia species followed the order of Mn, Fe, Zn, Cu, Se, but the content of Fe was higher than that of Mn in C. azalea. The contents of Pb, Cd, As and Hg of the four Camellia flower were all lower than the permissible amount. 相似文献
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The rapid propagation technology of Dendrocalamus minor var. amoenus was studied by investigating the effects of some factors such as selection of explant, phytohormone, culture method etc. The results show that: The best month for explant collection of D. minor var. amoenus is May and June. The best position for explant collection is middle-upper part knot of semi-lignification branch. The clump shoot could be induced in medium with 3/4MS+BA 4 mg·L-1+KT 1 mg·L-1+CW 100 mL·L-1. The optimal medium for subculture of D. minor var. amoenus is 3/4MS+BA 2 mg·L-1+KT 1 mg·L-1+CW 100 mL·L-1. Liquid medium is beneficial for improving growth condition and proliferation rate of clump shoot. The medium 1/5MS+IBA 8 mg·L-1+ NAA 4.5 mg·L-1 + KT 0.1mg·L-1 is a relative suitable rooting medium for D. minor var. amoenus, with the rooting method of synchronized treatment first and then rooting. The survival rate of seedlings could be higher than 90% in substrate of fine river sand∶peat soil=3∶ 1. 相似文献
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通过对山苍子幼嫩叶片、顶芽、花蕾3种组织的DNA提取效果分析和对影响酶切及选择性扩增效果的4个主要因素(酶切时间、Mg2+浓度、dNTPs浓度、引物浓度)的比较研究,建立了适合于山苍子AFLP分析的技术体系。结果表明,山苍子的顶芽是较好的DNA提取材料;山苍子基因组DNA经5 U EcoR I和5 U Mse I酶切1 h即可完全酶切;最佳的选择性扩增体系为20 μL反应体系中含有1.0 U rTaq聚合酶、2.0 μL 10×PCR缓冲液、1.8 μL 25 mmol·L-1MgCl2、1.4 μL 2.5 mmol·L-1dNTP、100 ng·μL-1引物各1.0 μL。使用该反应体系获得了清晰、稳定的DNA指纹图谱,并筛选出10对多态性较好的AFLP引物组合,为利用AFLP标记技术进一步开展山苍子种群遗传结构、遗传分化等研究奠定了基础。 相似文献
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All factors affecting the PCR system in Pinus massoniana Lamb were investigated one by one.The results show that the optimum PCR system of EST-SSR were:2μL 10×Buffer(Mg2+ Free), 30 ng template DNA,0.187 5 mmol/L dNTPs, 3.75 mmol/L Mg2+,8 pmol primer pair,1.0 U Taq DNA poly-merase in total 20μL reaction system. Finally,a total of 11 isolates of P.massoniana was used for testing the stability of the PCR amplification.The results prove that the optimum PCR system was stable,reliable,highly repetitive. 相似文献
10.
Pink Peace’rose was used to study the optimum conditions for transferring the SeNHX1 gene into the callus. The results showed that the optimal medium was MS+2,4-D 5.0 mg·L-1 + TDZ 0.5 mg·L-1. Agrobacterium tumefaciens-mediated transformation was able to take the target gene into callus and the blue spots were found. The optimum conditions for the transient expression of gusA gene are as following: bacterium density of OD was 0.5, infection time was 20 min, culture time was 3 days. Adding 100 μmol·L-1 AS, the frequency of transient expression of GUS gene was the highest, which reached about 85% in present study. 相似文献
11.
上犹县有机油茶栽培技术 总被引：1，自引：0，他引：1
近几年,素有"东方橄榄油"美誉的茶油越来越受到人们的青睐,油茶产业发展方兴未艾,有机油茶栽培也正在试点推广。文章介绍上犹县有机油茶栽培技术要点,以期为有机油茶的发展提供参考。 相似文献
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油茶是淳安的传统经济林树种,也是绿化造林的先锋树种。淳安县现有油茶10万亩,由于树龄老化,品种混杂,经营粗放,经济效益低下,制约了油茶产业的发展。近年来,淳安县通过推广油茶良种化、进一步扩大油荼规模、开展原有油茶园改造示范和油茶油品牌建设等措施,油茶产业发展有了明显的提升。目前既有领导高度重视、良好的基础条件和增长潜力、市场前景广阔、集生态观赏经济多功能于一体,尤其是生态景观效益显著等有利条件,也有良种苗木供应不上不利因素。通过广泛宣传油茶的优点和发展油荼的重要意义、科学规划油茶产业、改造现有油茶林努力提高单位面积产量、充分利用林地空间开展山核桃林地油茶生态套种绿化、加快油茶良种繁育基地建设推广油茶优良新品种、发展油茶庭院经济美化乡村环境、改进加工工艺发挥品牌效益、努力拓宽资金渠道等措施,力争到2015年全县油茶基地面积达到15万亩,油茶亩产量实现翻一番,油茶产业产值达到1亿元以上,实现油茶产业可持续发展。 相似文献
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至2012年,经过40 a发展,赣南脐橙面积11.87万hm2,产量125万t,面积世界第一,年产量世界第三,成为全国最大的脐橙主产区,2012年8月评为全国驰名商标;赣南也是全国油茶老产区,2009年9月国家发改委、国家林业局确定的全国100个油茶示范基地县中,赣南有10个基地县,分别占全国10%,全省43.5%,这是赣南发展油茶的利好政策,大好机遇。借鉴赣南脐橙的发展理念,促进赣南油茶产业发展,通过5～8a的不断努力,力争“赣南茶油”评为驰名商标,富裕赣南农村、农民。 相似文献
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宣城市油茶产业发展对策浅析 总被引：1，自引：0，他引：1
宣城市地处油茶适生区,发展油茶产业的优势与劣势并存。立足基地建设,面向长三角市场,化江浙沪皖四地交汇的交通优势为产业优势,引进油茶精深加工产业集群,是本市油茶产业的发展出路。 相似文献
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肇庆市宜林地资源充足,气候条件适宜种植油茶,油茶栽培历史悠久,具有发展油茶产业的有利条件,但目前全市油茶林面积小、产量少、多为散生、不成规模,应促进油茶产业化发展.为此,提出了肇庆市发展油茶产业要重视栽培区域与立地、油茶品种与苗木、茶油产量与品质等3大关键科技问题.油茶基地规划与建设务必考虑海拔、坡度、坡向等地形因子及... 相似文献
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江西省油茶主产区土壤理化性状调查研究 总被引：1，自引：0，他引：1
调查江西省油茶主产区近年新造林地与自然林分的土壤理化状况,结果表明：油茶林地地貌类型分布主要集中在中丘、高丘和山地;油茶林地土壤类型主要是红壤,土层较厚,层次不清、腐殖质少、块状结构、质地较粘;山地养分一般高于丘陵,丘陵高于岗地,平原人为耕作影响养分含量较高。N、K、pH值和代换性盐基随海拔增高呈升高趋势,P变化不明显;土壤养分差异比较大,大部分养分指标最大值与最小值相差约10倍。油茶林地土壤养分含量均比较低,尤其是P,其次是N、K,盐基代换量也比较低,林地的保水保肥能力差。 相似文献
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广西油茶产业中存在的问题与对策 总被引：3，自引：0，他引：3
刘频 《中南林业调查规划》2010,29(2):21-23
油茶是广西主要栽培经济树种之一,现有油茶种植面积36.7万hm2。介绍广西油茶产业建设概况,分析油茶产业中存在的主要问题,提出油茶产业发展的主要对策。 相似文献