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1.
Shihong Xiao Huiming You Weibin You Jinshan Liu Changtang Cai Jianqin Wu Zhirong Ji Shihua Zhan Zhesen Hu Zhongrui Zhang Dongjin He 《林业研究》2017,28(3):521-528
The rhizosphere, distinct from bulk soil, is defined as the volume of soil around living roots and influenced by root activities. We investigated protease, invertase, cellulase, urease, and acid phosphatase activities in rhizosphere and bulk soils of six Nothotsuga longibracteata forest communities within Tianbaoyan National Nature Reserve, including N. longibracteata + either Phyllostachys pubescens, Schima superba, Rhododendron simiarum, Cunninghamia lanceolata, or Cyclobalanopsis glauca, and N. longibracteata pure forest. Rhizosphere soils possessed higher protease, invertase, cellulase, urease, and acid phosphatase activities than bulk soils. The highest invertase, urease, and acid phosphatase activities were observed in rhizosphere samples of N. longibracteata + S. superba. Protease was highest in the N. longibracteata + R. simiarum rhizosphere, while cellulase was highest in the pure N. longibracteata forest rhizosphere. All samples exhibited obvious rhizosphere effects on enzyme activities with a significant linear correlation between acid phosphatase and cellulase activities (p < 0.05) in rhizosphere soils and between protease and acid phosphatase activities (p < 0.05) in bulk soils. A principal component analysis, correlating 13 soil chemical properties indices relevant to enzyme activities, showed that protease, invertase, acid phosphatase, total N, and cellulase were the most important variables impacting rhizosphere soil quality. 相似文献
2.
杉木根际与非根际土壤酶活性比较 总被引：19，自引：2，他引：19
杉木根际与非根际土壤酶活性比较陈ｆ竣（中国林业科学研究院林业研究所北京１０００９１）李传涵（华中农业大学土化系武汉４３００７０）关键词杉木,根际土壤,土壤酶,杉木中毒由于林木生长的影响,使得林木根际土壤ｐＨ值、养分含量、微生物数目、生化活性等性质发生... 相似文献
3.
Lianghua Qi Xudong Zhang Zhenhua Peng Jinxing Zhou 《Frontiers of Forestry in China》2009,4(4):443-449
With the aid of canonical correlation analysis, the relations among soil nutrients, soil microorganisms, and soil enzyme activities were studied in vegetation restoration areas of degraded and eroded soils in the Nverzhai watershed in northwestern Hunan. The main results were as follows: the key factors in soil nutrients, microorganisms, and enzyme activities were N and P elements, number of bacteria, carbon and nitrogen in soil microbial biomass and the activities of urease, polyphenol oxidase, phosphatase, and invertase. The activities of urease and polyphenol oxidase are related to the inversion of N and P elements that had important impact on the accumulation of carbon and nitrogen in soil microbial biomass. Moreover, the activities of urease, polyphenol oxidase, and phosphatase could promote carbon accumulation in microbial biomass; however, invertase activities inhibited the accumulation of microbial biomass nitrogen. On the other hand, urease activities were beneficial to the N element content in soils but unfavorable for P elements. There is a negative relation between polyphenol oxidase activity and N element content. For every canonical variable group, the tendencies of soil nutrients, microorganisms, and enzyme activities to accumulate in different soil layers in different vegetation restoration communities could offer some scientific basis for the diagnosis of the health of the soil and the site type division in the process of vegetation restoration. 相似文献
4.
以大鹤国有防护林场3种不同林龄(15、23、34 a)的湿地松林为研究对象,研究了不同林龄湿地松林土壤酶活性及其养分含量变化特征。结果表明:土壤pH值随着林龄增加呈先降低后升高,随着土层深度的增加逐渐升高。随着林龄的增长,土壤有机质先增加后降低;土壤水解氮、有效磷和速效钾含量逐渐降低。土壤中的脲酶、蔗糖酶、过氧化氢酶和多酚氧化酶活性随林龄的增长逐渐降低;酸性磷酸酶活性仅在表层土随着林龄的增长逐渐降低,而随着土层的加深表现为先增长后降低。土壤养分和酶活性随土层深度的变化均存在显著性变化。相关分析表明,土壤脲酶、蔗糖酶和酸性磷酸酶均与土壤有机质、水解氮、有效磷和速效钾存在极显著正相关(P<0.01),多酚氧化酶与速效钾呈显著负相关(P<0.05)。滨海地区湿地松在林龄较低阶段土壤养分较为充足,随着林龄的增长,土壤肥力逐渐下降,不利于森林可持续发展。 相似文献
5.
广西英罗港不同红树植物群落土壤理化性质与酶活性的研究 总被引：60，自引：5，他引：60
本文对广西英罗港自然保护区不同红树植物群落土壤主要性质进行了较系统的研究。结果表明 ,英罗港不同红树植物群落土壤理化性质和酶活性均存在明显差异 ,土壤粘粒粉粒含量和有机质、全氮、水解氮、全磷、速效磷及盐分含量为 :木榄群落 >红海榄群落 >秋茄群落 >桐花树群落 >白骨群落 ;土壤蔗糖酶、蛋白酶、脲酶、酸性磷酸酶 4种水解酶和过氧化氢酶的活性大小顺序为红海榄群落 >木榄群落 >秋茄群落 >桐花树群落 >白骨壤群落 ,多酚氧化酶则与此相反 ;土壤养分含量和土壤水解酶以及多酚氧化酶活性均随剖面深度而降低 ,过氧化氢酶则呈相反趋势。英罗港红树植物群落土壤酶活性与土壤肥力因素密切相关 ,各土壤酶活性之间也存在不同程度的相关性。因此 ,土壤酶活性可以作为该土壤肥力的指标之一。 相似文献
7.
筇竹不同林分类型土壤酶活性的垂直分布特征 总被引：1，自引：1，他引：0
在云南省大关县木杆镇选择4种筇竹林分类型：天然筇竹—阔叶树混交林、天然筇竹—山茶混交林、天然筇竹—人工黄皮树混交林、人工筇竹—黄皮树混交林等，研究不同筇竹林分类型、不同土层深度(0~20、20~40和40~60 cm)的土壤过氧化氢酶、蔗糖酶、酸性磷酸酶和脲酶活性。结果表明：1)天然筇竹—阔叶树混交林的土壤过氧化氢酶、酸性磷酸酶和脲酶活性高于其他3种林分类型，人工筇竹—黄皮树混交林的土壤蔗糖酶活性高于其他3种林分类型，说明人工施肥处理对提高蔗糖酶活性有一定的作用；2)除天然筇竹—阔叶树混交林的蔗糖酶外，4种林分类型的土壤酶活性在土层0~20 cm达到最大值，并且随着土壤土层深度的增加而降低；3)除天然筇竹—人工黄皮树混交林的蔗糖酶和脲酶与过氧化氢酶、人工筇竹—黄皮树混交林和天然筇竹—山茶混交林的酸性磷酸酶与过氧化氢酶、天然筇竹—阔叶树混交林的酸性磷酸酶与蔗糖酶无显著相关外，其他酶活性之间均存在显著或极显著相关(P＜0.05或P＜0.01)。研究表明林分类型和土层深度是影响土壤酶活性的重要因素。 相似文献
8.
To improve the productivity of poplar plantations, a field experiment of split-plot design with four tree spacings and three poplar clones was established, and four soil enzyme activities and microbial biomass were monitored in the trial.Soil enzyme activities, in most cases,were significantly higher in topsoil(0–10 cm) than in lower horizons(10–20 cm).Soil cellulase, catalase and protease activities during the growing season were higher than during the non-growing season, while invertase activity followed the opposite trend.Soil invertase, cellulase and catalase activities varied by poplar clone but soil protease activity did not.Cellulase and protease activities in the plantation at 5×5 m spacing were significantly higher than in the other spacings.The highest catalase activity was recorded at 6×6 m spacing.At the same planting density, invertase activity was greater in square spacings than in rectangular spacings.Soil microbial biomass was also significantly affected by seedling spacing and poplar clone.The mean soil MBC was significantly lower in topsoil than in the lower horizon, while MBN showed the opposite pattern.Significantly positive correlations were observed among soil cellulase, protease and catalase activities(p0.01), whereas soil invertase activity was negatively and significantly correlated with cellulase, protease and catalase activities(p0.01).Soil microbial biomass and enzyme activities were not correlated except for a significantly negative correlation between soil MBC and catalase activities.Variations in soil enzyme activity and microbial biomass in different poplar plantations suggest that genotype and planting spacing should be considered when modeling soil nutrient dynamics and managing for long-term site productivity. 相似文献
9.
Phosphorus dynamics and solubilizing microorganisms in acid soils under different land uses of Lesser Himalayas of India 总被引：1，自引：0，他引：1
Ranjan Paul Raj Deo Singh A. K. Patra D. R. Biswas Ranjan Bhattacharyya K. Arunkumar 《Agroforestry Systems》2018,92(2):449-461
Although chemical and some soil physical properties have been studied under different land uses of the Lesser Himalayas of India, very limited information is available on soil biochemical properties. Hence we investigated phosphorus (P) fractions [total P (TP), inorganic P (Pi), organic P (Po), available P, microbial biomass P (MBP)], enzyme activities [dehydrogenase, phosphatases, phytase], phosphate solubilizing bacteria (PSB) and fungi (PSF), and their correlations of acid soils (0–15 and 15–30 cm depths) under different land uses (viz, organic farming, maize–wheat, apple orchard, undisturbed oak forest and uncultivated land of the Indian Himalayas). All land use systems differed significantly for the P fractions, except TP. The highest values for TP, Pi, available P and MBP were found in soils under oak forest and lowest in uncultivated land. However, Po content was highest in apple orchard. The organic farming (organic manures field under garden pea-french bean cropping system for > 10 years) maintained highest activities of dehydrogenase, acid phosphatase and alkaline phosphatase. The highest phytase activity and highest numbers of PSB (99 × 103 g?1 soil) and PSF (30 × 103 g?1 soil) were observed in the rhizosphere soils of oak forest. Significant relationships between soil P fractions and enzyme activities, except alkaline phosphatase, were recorded in surface soil layer. PSB and PSF population were also correlated significantly with P fractions and enzyme activities. This would lead us to understand the level of degradation of P pools due to cultivation over forest system and the suitable management practices needed for soil quality restoration. 相似文献
10.
11.
《林业研究》2020,(5)
Soil enzymes play a vital role in biogeochemical cycling and ecosystem functions. In this study, we examined the response of six soil enzymes to changes in physicochemical properties resulting from changes in season and vegetation and geological conditions. Catalase,urease, acid phosphatase, invertase, amylase, and cellulase not only promote carbon, nitrogen, and phosphorus cycling, but also participate in the decomposition of harmful substances. Thirty-six soil samples were collected from karst and non-karst areas in two different seasons and from three different types of vegetation in Yunnanprovince, southwest China. Both vegetation types and season had significant effects on soil physicochemical properties and enzyme activities. In the same plot, soil water content, electrical conductivity, organic carbon, total nitrogen, and total phosphorus increased in the rainy season, indicating enhanced microbial metabolic activity.With the exception of urease activity, the remaining five enzymes showed higher activity in the rainy season.Changes in activities between the two seasons were significant in all samples. In the same season, activity levels of soil enzymes were higher in karst areas than in non-karst areas, and higher in natural forest than in artificial forests.The transformative abilities of soil elements are higher in karst areas than in non-karst areas, and higher in natural forests than in artificial forests. Correlation analysis showed that the activities of the six enzymes correlated significantly; however, soil physical and chemical indices,such as organic matter, p H, and moisture, which are essential for enzyme activity, differed by season. Redundancy analysis also revealed that the main factors influencing enzyme activity differed between the two seasons.The results from this study provide a theoretical basis for further research on the restoration of natural ecological systems in karst landscapes. 相似文献
12.
桉树人工林地土壤酶活性与营养元素含量关系研究 总被引：4，自引：0，他引：4
运用典范相关分析 (canonicalcorrelationanalysis)对桉树人工林地土壤酶活性和土壤营养元素含量关系的研究表明 ,土壤过氧化氢酶、脲酶和蛋白酶活性与营养元素全P和全N含量关系最大 ,即正效应较大 ,它们对N、P的转化具有重要作用。转化酶活性在一定程度上对土壤养分K、P和Mg含量有一定的负相关作用。“综合土壤酶因子”可作为土壤肥力评价的一个指标 相似文献
13.
荧光假单胞菌与红绒盖牛肝菌共接种对杨树根际土壤酶活性及微生物多样性的影响 总被引：2，自引：0，他引：2
【目的】探究荧光假单胞菌与红绒盖牛肝菌共接种条件下NL-895杨根际土壤酶活性和土壤微生物功能多样性的变化,从微生态水平揭示溶磷细菌与外生菌根真菌互作对NL-895杨的促生机制。【方法】采用盆栽试验考察荧光假单胞菌JW-JS1与红绒盖牛肝菌(Xc)共接种后NL-895杨根际土壤酶(酸性磷酸酶、脱氢酶和转化酶)的活性变化,并通过BIOLOG微孔板法分析共接种对NL-895杨根际土壤微生物群落和功能多样性的影响。【结果】从整体趋势来看,不同接种处理后NL-895杨根际土壤酶活性均有所增强,土壤酸性磷酸酶、脱氢酶和转化酶活性均大于对照,其中,共接种JW-JS1+Xc处理对土壤酸性磷酸酶和转化酶活性的促进最为明显;接种150天后,不同接种处理土壤微生物的AWCD值均高于CK(培养24～48 h除外),且随着培养时间的延长呈先上升后稳定的趋势;培养96 h后,共接种JW-JS1+Xc处理土壤微生物McIntosh优势度指数和碳源利用丰富度指数显著高于单接种Xc或JW-JS1和CK(P<0.05),而Shannon丰富度指数和Simpson优势度指数显著低于单接种Xc或JW-JS1和CK(P<0.05);不同接种处理NL-895杨根际土壤微生物对BIOLOG微孔板中6大类碳源的利用能力差异显著(P<0.05)。【结论】荧光假单胞菌JW-JS1与外生菌根真菌Xc共接种对NL-895杨根际土壤酶活性和土壤微生物功能多样性的提高具有一定的促进作用,有利于其土壤肥力的改善,进而促进NL-895杨的生长。与单接种相比,溶磷细菌与外生菌根真菌共接种可更有效地提高杨树根际土壤酶活性和土壤微生物多样性。 相似文献
14.
不同海拔毛竹土壤酶活性与土壤理化性质关系的研究 总被引：4，自引：0，他引：4
以江西省大岗山地区的毛竹林为研究对象,利用相关分析和主成分分析对不同海拔(300、400、500、600、700m)土壤纤维素酶、蔗糖酶、淀粉酶、β-葡糖苷酶和多酚氧化酶活性的变化特征进行了分析,探讨了其与土壤理化性质的关系。结果表明:土壤化学指标表现出随海拔的升高而增加的变化特征,除碱解氮外,其他各指标均处于较低水平,但物理指标的变化各不相同;随海拔升高,β-葡糖苷酶与多酚氧化酶活性呈现出增加的趋势,而纤维素酶、蔗糖酶和淀粉酶活性无明显变化规律;几种土壤酶对土壤肥力的指示效果不同,β-葡糖苷酶在评价毛竹林土壤肥力上具有重要指示效果,其后依次为蔗糖酶和多酚氧化酶、纤维素酶,但淀粉酶不适宜用来评价土壤肥力状况;通过主成分分析计算综合得分,不同海拔毛竹土壤肥力随海拔的升高而增加。 相似文献
15.
杉木人工林土壤酶活性对氮沉降的响应 总被引：1，自引：0，他引：1
对12年生杉木人工林开展N0(0kgN·hm-2a-1)、N1(60kgN·hm-2a-1)、N2(120kgN·hm-2a-1)和N3(240kgN·hm-2a-1)4种水平的模拟氮沉降试验,探讨亚热带森林土壤酶(过氧化氢酶、蔗糖酶和脲酶)活性对氮沉降增加的响应。试验采取2种施氮方式,即在缓冲区采取1年1次性施入氮,而在中心区每月施入等量氮。结果表明:缓冲区1次性施氮后30天内每10天土壤酶活性变化趋势和中心区按月施氮后酶活性动态均表现为N1始终促进3种酶活性(相对于N0处理);N1和N2处理对土壤酶活性的影响依施氮时间和土层深度不同而异,没有表现出明显的促进增加或抑制作用;随土层深度增加,3种酶活性均表现出明显降低趋势。 相似文献
16.
Ginkgo (Ginkgo biloba L.) is a traditional economic tree species in China, and often cultivated in agroforestry systems. The objective of the study
was to examine the effects of different Ginkgo and crop species combinations on soil fertility. We established two Ginkgo
and crop species systems: Ginkgo + wheat + soybean (G+W+S), Ginkgo + rape + soybean (G+R+S), and one Ginkgo + mulberry (G+M)
system, one pure mulberry plantation (M), and one crop systems of rape + soybean (R+S) in the field. Soil chemical properties
and enzymes activities were measured 4 years after planting. These soil chemical properties and enzyme activities were used
as soil fertility indicators (FI). Soil fertility of the five planting systems was finally evaluated by using improved Analytic
Hierarchy Process (AHP). The result showed that soil chemical properties (including pH, organic matter, total N, hydrolysable
N, available P and K, total K) and soil enzyme activities (including catalase, sucrase, urease, dehydrogenase, phosphatase,
polyphenol oxidase and protease) in the five planting systems were significantly different from each other (P = 0.0237). The above parameters were also different at different soil depths. The concentration of total N, P, organic matter,
available P and K of soil decreased significantly with soil depth (P = 0.0146), however, pH increased. The concentrations of organic matter, total N, hydrolysable N, available P and K of soil
under rape + soybean (R+S) was lowest among the five planting systems. The activities of sucrase, urease, dehydrogenase, phosphatase
and protease decreased with soil depth, however, the activity of catalase and polyphenol oxidase increased. Except for catalase,
the activities of these enzymes in the R+S system were the lowest among the five planting systems. The integrated evaluation
of soil fertility showed that soil fertility indicators (FI) were significantly different, and the FI values for the five
systems followed order: G+M (0.847) > G+W+S (0.446) > M (0.399) > G+R+S (0.343) > R+S (0.211). These results indicated that
adoption of a Ginkgo-crop combination could lead to increased long-term sustainability of soil fertility by improving levels
of soil organic matter, pH, available nutrient and soil enzyme activity. 相似文献
17.
胡桃楸落叶松纯林与混交林中根际土壤的养分特征(英文) 总被引：6，自引：0，他引：6
与非根际土壤相比较,研究了胡桃楸落叶松纯林与混交林中根际土壤pH值、有机质及氮 、磷、钾等养分元素的特征。结果表明：各林分中,除胡桃楸纯林外,根际土壤的pH值均不 同程度低于非根际土壤;除混交林中落叶松根际土有机质含量略低于非根际土外,根际土壤 有机质含量均高于非根际土壤;根际土壤存在氮的相对累积,磷和钾在根际则具有一定的亏 缺趋势,其程度与树种相关;各林分下,氮、磷、钾在根际中均得以活化,表现为根际土壤 速效氮、磷、钾含量偏高;混交林中落叶松根际速效氮含量明显高于其纯林,而混交林中胡 桃楸根际速效磷、钾含量明显高于其纯林。 相似文献
18.
Responses of labile soil organic carbon and enzyme activity in mineral soils to forest conversion in the subtropics 总被引：1，自引：0，他引：1
Aims
Globally, extensive areas of native forest have been almost replaced by plantations to meet the demands for timber, fuel material and other forest products. This study aimed to evaluate the effects of forest conversion on labile soil organic C (SOC), soil respiration, and enzyme activity, and to quantify their relationship in subtropical forest ecosystems.Methods
Surface mineral soil (0–20 cm) was collected from a Cunninghamia lanceolata Hook. plantation, Pinus massoniana Lamb. plantation, Michelia macclurei Dandy plantation, and an undisturbed native broadleaf forest. Soil microbial biomass C, dissolved organic C, permanganate-oxidizable C, basal respiration, and six enzyme activities were investigated.Results
Soil microbial biomass C was higher by 45.9 % in native broadleaf forest than that in M. macclurei Dandy plantation. The ratio of soil microbial biomass C to total SOC was 27.6 % higher in the M. macclurei Dandy plantation than in the native broadleaf forest. The soil respiration increased by 25.2 % and 21.7 % after conversion from native broadleaf forest to P. massoniana Lamb. and M. macclurei Dandy plantations respectively. The effects of forest conversion on the soil enzyme activities differed among the tree species. Soil microbial biomass C had higher correlation with soil respiration than with the other SOC fractions. Moreover, soil microbial biomass C was positively correlated with urease and negatively correlated with cellulase activity. Soil respiration had higher correlation with soil microbial biomass C, dissolved organic C and permanganate-oxidizable C.Conclusion
Forest conversion affected the soil microbial biomass C, soil respiration, invertase, cellulase, urease, catalase, acid phosphatase, and polyphenol oxidase activities, but their response depended on tree species. Soil respiration was mainly controlled by labile SOC, not by total SOC. 相似文献19.
20.
在江汉平原选择有代表性的5~6龄的杨树林林地11块,并以林外土壤为参照,研究林下土壤酶(蔗糖酶、脲酶、蛋白酶、中性磷酸酶、过氧化氢酶、多酚氧化酶)活性和3大类微生物(真菌、细菌、放线菌)数量的年度动态变化。结果表明,林内与林外土壤酶及微生物的时间变化格局基本一致,林内土壤不同微生物数量和酶活性的变化反映了杨树凋落物数量变化的年节律性;在3个不同月份中,林下土壤大多数肥力特征指标均高于林外;从一年的观察数据来看,栽培杨树可在一定程度上提高林地土壤肥力,不会导致林下土壤肥力的恶化;在微碱性土壤上杨树对土壤肥力的正面作用比酸性土壤更好。 相似文献