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1.
Catharanthus roseus is a seasonal to perennial garden plant and the exclusive source of the anticancer drugs vincristine and vinblastine. Its horticultural importance is due to the compound racemose inflorescence architecture of branches in which pairs of prominent flowers are subtended by one of the two leaves per node. Here is reported the construction of a molecular framework genetic linkage map and mapping on it of the LEAF-LESS INFLORESCENCE (LLI) locus. It is quantitatively shown that the adult lli mutant plants produce altered inflorescence of improved horticultural value, wherein axes are excessively branched, two flowers are formed per node that are bare of subtending leaves, and several times more open flowers are displayed each day, as compared to LLI plants.  相似文献   

2.
Catharanthus roseus L. (G.) Don. (Apocynaceae) is an important dicotyledonous medicinal plant as it is the sole source of vincristine and vinblastine that are used against a variety of cancers. Quantitative estimation of vincristine was carried out using high performance liquid chromatography (HPLC) in various in vitro grown tissues; calluses (embryogenic and non-embryogenic callus), embryogenic stages (proliferated, matured and germinated embryos), somatic embryo derived plantlets (leaf, root and whole plant) and leaves of ex vitro developed plantlets. The yield in those in vitro and ex vitro-developed tissues was monitored for 30 weeks. Except at an early lag period, vincristine production was detected up to 20–25 weeks old plant samples. Vincristine content was very high in leaf callus and germinated embryos. Leaves of in vitro raised plants showed higher amount of vincristine when compared to ex vitro-developed leaves of similar age. Vincristine production was tissue specific and age dependent that was discussed in detail in this present communication.  相似文献   

3.
An efficient indirect somatic embryogenesis and Agrobacterium-mediated transformation protocol for Limonium sinense has been established, wherein neomycin phosphotransferase II (npt II) and β-glucuronidase (GUS) genes were used as selectable and screenable markers, respectively. The efficiency of plantlet regeneration from transformed tissue was compared between direct embryogenesis from leaf and indirect embryogenesis from callus. Embryogenic callus (EC) was initiated from leaf explants on MS medium supplemented with 6.7 μM 2,4-D and 2.22 μM BA. The somatic embryos were induced, matured, and germinated when ECs were transferred onto MS medium supplemented with 4.44 μM BA and 1.07 μM NAA. Agrobacterium tumefaciens strain LBA 4404 containing the vector pBI121 was used for the transformation. Transient GUS expression frequency was evaluated and putative transgenic plants were successfully grown on culture medium in presence of kanamycin (80–100 mg L?1). PCR analysis of putative transgenic plants confirmed the presence of GUS and nptII genes. The transformation efficiency obtained through indirect embryogenesis from calluses (4%) was much higher than through direct embryogenesis from leaf explants (0.9%).  相似文献   

4.
Drought is a major abiotic stress responsible for severe crop losses worldwide. Development of new crop varieties with increased drought tolerance is one way to increase crop productivity. The aim of this study was to characterise the diversity of nine accessions belonging to Amaranthus tricolor and A. cruentus, in response to drought stress using a dry-down protocol to characterise the transpiration efficiency (TE). Plants were subjected to either a gradual dry down or well-watered conditions. Results showed that TE was significantly higher (P < 0.01) in water-deficient (WD) plants compared to water-sufficient (WS) plants, 2.40 g kg?1–7.13 g kg?1 and 2.19 g kg?1–4.84 g kg?1, respectively. There was no significant difference in the fraction of transpirable soil water (FTSW) threshold decline between the amaranth genotypes. TE was highly correlated with yield under both WS (r = 0.89, P < 0.001) and WD conditions (r = 0.662, P < 0.001), and negatively correlated with root-to-shoot ratio under both WS (r = ?0.488, P < 0.05) and WD conditions (r = ?0.460, P < 0.05). Significant genotypic differences were seen for growth rate and stress susceptibility index (SSI). The result obtained in this investigation underline the need to identify genotypic variation in water use efficiency in amaranth.

Abbreviations: FTSW: Fraction of transpirable soil water; NTR: normalised transpiration rate; SSI: Stress susceptibility index; TE: transpiration efficiency; WHC: water holding capacity; WD: water-deficient; WS: water-sufficient.  相似文献   

5.
1 品种选择及播期确定 1.1 砧木的选择 选择砧木应首先考虑与接穗要有较好的亲合力,即接穗与砧木嫁接后能够较好的愈合,发育旺盛以保证嫁接苗生产管理正常.其次要求砧木具有良好的适应能力,抗逆性强,抗病力强,且嫁接后不影响接穗果实品质.经过多年试验摸索,选用杂交白籽南瓜作为砧木即能提高抗病性又对果实品质影响小.  相似文献   

6.
Cardoon (Cynara cardunculus L.) is considered as one of the most suitable energy crops for Southern Europe. The aim of this work was to outline the effects of organic fertilisers on the productivity and the global warming potential (GWP) on cardoon production. Six fertilisers (N 100 kg ha?1, N 50 kg ha?1, Compost 30 t ha?1, Compost 15 t ha?1 + N 25 kg ha?1, 3 t ha?1 of defatted oilseed meals of sunflower, 3 t ha?1 of defatted oilseed meals of Brassica carinata), and unfertilised control, were evaluated on two cultivars (‘Gobbo di Nizza’ and ‘Altilis 41’) in a split-plot experiment. Defatted oilseed meal of sunflower recorded higher total dry weight (+10%), seed yield (+17%), nitrogen use efficiency (+14%) and better GWP (?66%) compared to the other organic fertilisers and performing as well as N 100 kg ha?1. Altilis 41 cultivar showed the highest aboveground total dry weight (10 t ha?1 y?1), seed yield (1.7 t ha?1 y?1), stalk dry weight (7 t ha?1 y?1) and head dry weight (3 t ha?1 y?1). Our results highlighted that by combining suitable cultivar and fertilisation strategies, it could be possible to increase the production sustainability of C. cardunculus.  相似文献   

7.
以温室内生长的红长春花[Catharanthus roseus(L.G.Don]和白长春花[Catharanthus roseus(L.)G.Don cv.Albus]为研究对象,通过对长春花的净光合速率日变化、叶绿素含量、光响应曲线及几项重要的光合参数等的研究,分析比较红长春花和白长春花光合生理的特性.结果表明,红长春花光合能力及利用光能的能力均强于白长春花.具体表现在:红长春花的叶绿素含量高于白长春花;红长春花的净光合速率高于白长春花;长春花在对强光的利用上表现出喜光植物的生理特性;红长春花的利用强光的能力高于白长春花;两种长春花净光合速率Pn日变化曲线趋势相同,均为单峰型.  相似文献   

8.
The influences of plug size, planting density, and mulch colour on responses to photoperiod and nitrogen conditioning of ‘Albion’ strawberry (Fragaria x ananassa Duch.) plants were evaluated in off-season field production in New Jersey, USA. Three different sized plug plants (7.6 cm square pots, 24-cell plug trays, or 50-cell plug trays) were conditioned with natural days (ND, natural daylength) or long days (LD, natural daylength supplemented with 24 h incandescent radiation) for one week followed by ND plus 100 ppm nitrogen (N) for 4 weeks or LD plus 800 ppm N for 4 weeks. Plants were then established in plasticulture on black, white, or silver mulch. In a second experiment, plug plants (439 cm3) were conditioned with LD and nitrogen then established in plasticulture on black, white, or silver mulch at 3 planting densities (0.46, 0.23, and 0.16 m2?plant?1 (2, 4, and 6 plants?m?2)). Fruit were harvested from August through October in both experiments. Conditioning enhanced off-season fall production. White plastic enhanced survival, productivity, and fruit size and larger plugs were more productive than smaller ones. If smaller plugs are desired, conditioning prevents the reduction in yield observed with non-conditioned smaller plugs.  相似文献   

9.
Fertigation has the potential to reduce extra chemical load by improving nutrient and water use efficiency of coconut (Cocos nucifera L.), but studies demonstrating the fertilizer reduction through drip irrigation in comparison to conventional ring basin method are rare in the Eastern Indo-Gangetic Plains (IGP) of South Asia. A long-term field experiment was conducted during 2007–2013 in West Bengal, India, to study the effect of fertigation on coconut var. DXT. The experiment was laid out in a randomised complete block design (RCBD) with six treatments (control – no fertilizers and water applied with drip irrigation; 25%, 50%, 75% and 100% of the recommended dose of fertilizer (RDF), each applied with drip irrigation; and 100% of the RDF and water applied with ring basin method of irrigation (i.e., conventional method)). Nuts yield was significantly higher for 75% of RDF (24.44 t ha?1 year?1) followed by 100% of RDF, each drip irrigation (23.79 t ha?1 year?1) compared to control (21.89 t ha?1 year?1). Copra yield was significantly higher for 75% of RDF (3.19 t ha?1) compared to 100% of RDF (3.12 t ha?1) and no fertilizer (1.87 t ha?1). Nitrogen (N), phosphorus (P) and potassium (K) contents of soil increased by 4.9%, 10.4% and 9.4%, respectively, with 75% of RDF applied through drip irrigation. Microbial population showed inverse relationship with amount of fertilizer application. The most water-use efficient fertigation treatment was 75% RDF (13.48 kg copra m?3) followed by 100% RDF (13.18 kg copra m?3) with drip irrigation as compared to conventional way soil application of fertilizers through ring basin method of irrigation (4.23 kg copra m?3). Role of N on yield variability was most prominent by both available soil N status (R2 = 0.49**) and leaf N concentration (R2 = 0.51**). The study indicated that there is a great scope for reducing the N, P and K fertilizers by up to 25% of the present RDFs for coconut when applied through drip irrigation compared to ring basin method of irrigation for its higher productivity and profitability through efficient use of nutrients and water in the Eastern IGP of South Asia.  相似文献   

10.
The current analysis describes an improved protocol for somatic embryogenesis and plant regeneration in oil palm (Elaeis guineensis) through liquid medium, and assesses the influence of successive subcultures during induction of calluses in three Brazilian oil palm varieties. Calluses were induced in a Murashige and Skoog (MS) medium with 450 Picloram, 0.5 g L?1 glutamine, 2.5 g L?1 activated charcoal, 30 g L?1 sucrose, and solidified with 2.5 g L?1 Phytagel. In a first experiment, the effect of continued subculture of explants every 30 days to fresh culture medium was determined. During a second experiment, part of the embryogenic calluses obtained were transferred to a liquid medium under agitation, consisting of MS with 5 µM picloram or 2,4-dichlorophenoxyacetic acid (2,4-D). After 210 days, the calluses were transferred to semi-solid media for differentiating somatic embryos. It was observed that continued subculture of explants monthly was a determinant in stimulating and improving the formation of embryogenic calluses. Embryogenic calluses in liquid medium with 2,4-D significantly improved the percentage of differentiated somatic embryos (up to 80.2%), with the largest amount of torpedo embryos (8.3 per callus). Regenerated plants with roots were individualised and transferred to a greenhouse, with close to 95% survival.  相似文献   

11.
Capsicum spp. is a commercially important crop of the Solanaceae family, well-known for its multipurpose use as a vegetable, spice, medicinal and ornamental plants. The genus Capsicum is a recalcitrant species in terms of in vitro morphogenesis and plant regeneration. An efficient method was developed for multiple shoot regeneration in 10 cultivars of Capsicum collected from diverse geographical regions of India and Mexico. Seeds germinated in vitro on a half-strength Murashige and Skoog (MS) medium supplemented with 3.0 % sucrose. Nodes of the in vitro germinated seedlings were used as explant for micropropagation. The combination of the 6-benzylaminopurine, indole-3-acetic acid, and spermidine was found to be the best for multiple shoot induction. However, the optimum responcse varied accompanied by different cultivers with maximum 8.9 ± 0.52 (Capsi-10) to 15.3 ± 0.69 (Capsi-5) multiple shoot per explant. Depending on the cultivar, multiplied shoots were successfully rooted with maximum 18.4 ± 0.20 (highest for Capsi-9) to 36.8 ± 0.29 (highest for Capsi-5) roots per shoot on half-strength MS medium supplemented with 2.0 mg l?1 indole-3-butyric acid, 1.0 mg l?1 α-naphthalene acetic acid, and 1.5 mM spermidine. Finally, the micropropagated plantlets were acclimatized with 40.0–86.7 % survival rate, depending on different cultivars.  相似文献   

12.
The research was conducted in the pummelo growing areas of Thailand. The results showed that the dropped fruits had a statistically smaller fruit size (7.25 cm) than the retained fruit (9.35 cm; P < 0.05) at 4 months after fruit set. Moreover, the dropped fruits had an abnormal fruit shape when compared to the retained fruits. Consideration of fruit and leaf carbohydrate concentrations showed that the retained fruits had statistically higher carbohydrate concentrations (262 mg g?1) than the dropped fruits (203 mg g?1; P < 0.05). Meanwhile, the dropped fruits had statistically lower leaf carbohydrate concentrations (55 mg g?1) than those in the retained fruits (85 mg g?1; P < 0.05). The retained fruits also had statistically higher N, P, and K concentrations (1.6%, 1.1%, and 1.3%, respectively) than the dropped fruits (1.1%, 0.5%, and 1.0%, respectively; P < 0.05). Therefore, the causes of early fruit drop in pummelo could be insufficient carbohydrate and plant nutrients supplied for fruit growth and development.  相似文献   

13.
Hybrid breeding of Chinese jujube (Ziziphus jujube) is limited by embryo abortion. Improving the success of embryogenic progeny and immature embryo culture techniques could increase the breeding efficiency of new jujube varieties. We optimised the current immature embryo culture techniques by using immature embryos before the globular stage of Chinese jujube variety Lengbaiyu. The optimal growth media were as follows: for 30-day-old immature embryo growth, MS + IBA 0.8 mg·L?1 + ZT 0.5 mg·L?1 + GA3 7.0 mg·L?1 + NAA 0.2 mg·L?1 + LH 0.5 mg·L?1 + 5% sucrose, with 22.22% embryonic growth rate and 0.41% plantlet formation rate; and for young embryo differentiation, MS + TDZ (0.8 mg·L?1) + IAA (0.5 mg·L?1) + sucrose (3%), with 81.12% differentiation rate. Thus, regeneration via callus induction effectively improved the efficiency of embryo rescue from young immature embryos in Lengbaiyu, which might provide new approaches for breeding Chinese jujube.  相似文献   

14.
Summary

Shoot growth attributes, leaf anatomical changes and net photosynthetic rates were determined in young coffee plants fertilized at high and low levels and subjected to shade or full sunlight. High nitrogen supply increased plant height irrespective of the light level imposed during growth, and promoted a larger leaf area in plants of the shade treatment. Specific leaf weight was 15% higher in plants grown in full sunlight than in shaded plants, at both nitrogen levels. The number of leaves developed on the orthotropic stem increased significantly under full irradiance and high nitrogen availability. Leaves were 11% thicker ih unshaded plants than in shaded ones, because of the increased size of the palisade and spongy parenchyma tissues. More thylakoids per granum and more grana per chloroplast were observed in shade-grown plants, mainly in those given high nitrogen treatment. Total nitrogen content expressed on a leaf-area basis was higher in sun plants supplemented with nitrogen, whereas chlorophyll a and b and protochlorophyll contents increased in shade-grown plants. Net photosynthetic rate in high nitrogen plants reached 7.19 µmol CO2 m?2 s?1 in the full irradiance treatment, and 5.46 µmol CO2 m?2 s?1 in shaded plants. Maximum net photosynthetic rates in the low nitrogen plants were 5.28 and 4.90 µmol CO2 m?2 s?1 in sun and shade plants, respectively. Increased photosynthetic light saturation was observed in high irradiance plants (628 µmol m?2 s?1) relative to shade plants (359 µmol m?2 s?1) in the high nitrogen treatment. The same pattern was observed in low nitrogen plants. The apparent quantum yield of shade acclimated plants was 14% higher than in those grown in full sunlight. Our results indicate that coffee may be classified as a facultative shade species, exhibiting features of sun adapted plants coupled with shade acclimation attributes, this fairly high adaptive capacity to shade:sun transition being strongly influenced by the level of nitrogen nutrition given to the plants.  相似文献   

15.
Chinese jujube (Ziziphus jujuba Mill.) is a major fruit crop in Asia. In this study, response surface methodology (RSM) was successfully employed to establish a highly efficient in vitro propagation and regeneration system for the ‘Teapot’ jujube via shoot organogenesis. Among the tested factors, gibberellic acid (GA3) concentration showed the most significant positive effect. The pre-culture darkness timing and medium were also important factors for highly efficient shoot regeneration of the ‘Teapot’ jujube. The highest regeneration (> 75%) was achieved by 1 week in darkness and culture on wood plant medium (WPM) containing 0.25 mg·L?1 GA3, 0.5 mg·L?1 6-benzylaminopurine (BAP) and 0.1 mg·L?1 3-indoleacetic acid (IAA). In vitro-derived shoots rooted very well in the modified 1/2 Murashige and Skoog (MS) medium containing 0.4 mg·L?1 3-indolebutyric acid (IBA), resulting in a 100% rooting rate. These findings suggest that the RSM can be employed to optimise the protocols needed for successful in vitro plant regeneration of jujube cultivars, with potential applications in plant genetic transformation practices, polyploidy induction and germplasm preservation.  相似文献   

16.
ABSTRACT

Effect of 2,4-D and naphthalene acetic acid (NAA) on fruit drop reduction in pummelo cv. Thong Dee was investigated in the pummelo growing areas of Nakhon Pathom province, Thailand. Five similar sized and aged of pummelo trees were selected to set up the experiment. Ten mature branches with the same size from each pummelo tree were randomly selected around the canopy for 2,4-D (20 and 40 mg L?1), NAA (20 and 40 mg L?1) application and control. All treatments were applied to selected pummelo branches 2 times at full bloom and 2 months after fruit set. The results showed that 20 mg L?1 NAA (14.84%) and 40 mg L?1 NAA (12.26%) gave significantly higher percent of fruit retention at 6 months after fruit set. However, leaf total nonstructural carbohydrate concentration analysis showed that 40 mg L?1 2,4-D (104.86 mg g?1) and 20 mg L?1 2,4-D (96.55 mg g?1) gave significantly higher total nonstructural carbohydrate than those in control (78.44 mg g?1). For fruit quality, 40 mg L?1 2,4-D and 20 mg L?1 2,4-D gave the highest peel weight with 435.55 and 358.57 g, respectively, and 40 mg L?1 2,4-D gave the highest peel thickness with 20.25 mm, while 20 mg L?1 NAA gave statistically higher total soluble solid than those in 20 mg L?1 2,4-D and 40 mg L?1 2,4-D. Therefore, 20 mg L?1 of NAA sprayed 2 times at full bloom and 2 month after fruit set effectively reduced fruit drop and increased percentage of fruit retention in pummelo cv. Thong Dee.  相似文献   

17.
To study the effect of soil application of potassium humate (KH) (0, 70 and 140 Kg ha?1) on agronomic, biochemical attributes, and yield and its components on bean (Phaseolus vulgaris L.) cultivars ‘Bronco’ grown under two sites having different level of salinity (EC = 3.00 or 5.00 ± 0.20 dS m?1). Two field trials were conducted at the Agriculture Test Station, Faculty of Agriculture, Fayoum University, in 2014 and 2015. The obtained results indicated that addition of KH increased all of the studied growth parameters, i.e. plant height, number of leaves, leaf area, stem diameter and canopy dry weight. Moreover addition of KH significantly increased membrane permeability measurements. KH significantly increased chemical composition related to salt tolerant either inorganic e.g. N, P, K, and decreased Na and Cl contents of leaves or organic components e.g. chlorophyll a + b, carotenoids, anthocyanin total phenolics, total flavonoids and proline. DPPH radical-scavenging activity and yield and its components were increased. The soil application of KH was found to be highly effective at improving the growth and yield of bean plants by alleviating the inhibitory effects of soil salinity stress.  相似文献   

18.
Amana edulis (Miq.) Honda is an important medicinal plant with a variety of anti-cancer properties, and it is of great importance to improve its reproductive rate through micropropagation technology to meet increasing demand. In the present study, in order to establish a rapid in vitro bulblet propagation protocol for A. edulis, an L16 (45) orthogonal design was used to investigate the effects of sucrose, 6-benzyladenine (6-BA), α-naphthaleneacetic acid (NAA), and macro-elements on bulblet induction. The results show that the sucrose concentration was the crucial factor for A. edulis bulblet initiation, followed by 6-BA and macro-elements, while NAA had the weakest effect. The optimal medium for in vitro bulblet induction was Murashige and Skoog medium supplemented with 0.1 mg·L?1 6-BA, 0.01 mg·L?1 NAA, and 100 g·L?1 or 80 g·L?1 sucrose (pH 5.8), in which A. edulis shoot clusters (without roots) were cultured at 5(±2)°C for 35 d or 60 d and then incubated at 23(±2)°C for 90 d. The entire cultivation process occurred in the dark. The present study demonstrates that this protocol can be used for the propagation of A. edulis.  相似文献   

19.
Paclobutrazol (PBZ) is a member of the triazole plant growth inhibitor group that is responsible for inducing tolerance to a number of biotic and abiotic stresses. An experiment was, therefore, conducted to test whether PBZ application at various concentrations (0, 25, 50, or 75 mg L?1) through foliar spray or soil drench would protect pomegranate (Punica granatum) seedlings cv. Robab, subjected to freezing at ?3°C for 7 hours. PBZ improved the growth rate of pomegranate seedlings subjected to freezing stress and increased relative leaf chlorophyll content, chlorophyll fluorescence ratio, relative water content, soluble carbohydrate content, and enzyme activity of ascorbate peroxidase and guaiacol peroxidase compared with the control at the end of experiment. PBZ ameliorated the injury caused by freezing stress by inhibiting increases in proline content and leaf electrolyte leakage, which suggested that PBZ ameliorated the negative effect of freezing stress. The best protection was obtained from plants treated with PBZ at 50 or 75 mg L?1. The most evaluated parameters were not affected by PBZ application method.  相似文献   

20.
Grafting tomato (Solanum lycopersicum L.) onto disease resistant rootstocks has grown in use in North America over the past two decades. Rootstocks have traditionally been bred and used for their resistance packages to numerous soil-borne diseases but some rootstocks appear to improve scion yield and vigour in comparison to non-grafted plants in conditions lacking disease challenge. In this study, the tomato rootstocks ‘Maxifort’ or ‘RST-106’ were used to determine if vigour improved ‘Tribute’ scion traits, especially yield, and if marketable yield could be maintained in grafted plants at reduced nitrogen fertiliser inputs. Plants were grown in an open-field plasticulture production system at five rates of nitrogen from 0 kg ha?1 to 224 kg ha?1 applied via drip irrigation. Marketable yield was significantly affected by nitrogen rate and rootstock in both years. ‘Tribute’ grafted onto ‘Maxifort’ rootstock had the greatest, most consistent impact and increased marketable yield 15% and 30% in 2013 and 2014, respectively, compared to the non-grafted ‘Tribute’. Our findings suggest that some rootstocks can increase scion growth and yield but do not differentially respond to decreased nitrogen rates.  相似文献   

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