共查询到18条相似文献，搜索用时 213 毫秒
1.
水稻短根突变体ksr1的遗传分析和基因定位 总被引：1，自引：0，他引：1
从甲基磺酸乙酯诱变的Kasalath突变体库中,在苗期筛选到一个水稻短根突变体 ksr1,6 d苗龄时该突变体的根长只有野生型的20%左右,遗传分析表明该突变性状由一对隐性核基因控制.利用突变体与粳稻日本晴杂交发展的F2群体对突变基因进行了定位分析,初步定位结果显示目的基因 KSR1 与第4染色体上SSR标记RM1223连锁.在该标记附近进一步发展了8对SSR标记和2对InDel标记,将突变基因定位于InDel标记4-24725K和SSR标记RM17182之间,该区段物理距离为155 kb. 相似文献
2.
水稻着丝粒附近一个淡绿叶突变相关基因的定位分析 总被引：6，自引：0，他引：6
在T DNA插入水稻突变体库中,发现了一个以日本晴为遗传背景的温度钝感型淡绿叶突变体pgl2（pale green leaf 2 ）。遗传学分析表明该突变性状由1对单隐性核基因控制。利用突变体与籼稻品种龙特甫杂交,构建F2群体对突变基因进行精细定位。初步定位结果显示目的基因与第8染色体上SSR标记RM331连锁,在该标记附近发展了14对INDEL标记,将突变基因进一步定位于着丝粒上2.37 Mb的区间,并对该区间候选基因进行了分析。突变体叶绿素的总量与对照相仿,但是叶绿素a/b比值趋于1,明显低于对照。推测突变基因可能与叶绿素a、b间的转化有关。还就着丝粒中基因定位的引物设计方法进行了讨论。 相似文献
3.
在粳稻品种中花11的组培苗中发现1份可以稳定遗传的矮秆多分蘖突变体,相比野生型,突变体株高明显下降、分蘖能力明显增强。为定位控制该性状的基因,以该突变体和野生型杂交构建遗传群体,与籼稻黄华占构建定位群体,再利用SSR和In Del分子标记定位该基因。遗传分析表明,矮秆多分蘖突变体受1对隐性核基因控制,暂命名为htd(t)。利用F2代定位群体将突变基因定位于第4号染色体标记RM1345和ZM4-12之间,遗传距离分别为3.9和2.6 c M。序列分析表明,htd(t)可能为HTD1的等位基因。 相似文献
4.
水稻扭曲叶突变体rtl1是利用甲基磺酸乙酯（EMS）诱变粳稻品种日本晴获得的。在苗期,该突变体的叶片就表现出皱缩和扭曲状。将该突变体分别与籼稻品种台中本地1号和浙辐802进行配组。遗传分析表明该突变体性状受1对隐性单基因控制。通过混合分离法,找到了位于第4染色体上的紧密连锁SSR标记RM1155,通过新发展的多态性STS标记,最终将该基因定位在STS标记T1591和SSR标记RM1359之间,其遗传距离分别为0.48和0.96 cM。为进一步克隆该基因打下了基础。 相似文献
5.
水稻扭曲叶突变体rtll是利用甲基磺酸乙酯(EMS)诱变梗稻品种日本晴获得的.在苗期,该突变体的叶片就表现出皱缩和扭曲状.将该突变体分别与籼稻品种台中本地l号和浙辐802进行配组.遗传分析表明该突变体性状受1对隐性单基因控制.通过混合分离法,找到了位F第4染色体上的紧密连锁SSR标记RM1155,通过新发展的多态性STS标记,最终将该基因定位在STS标记T1591和SSR标记RM1359之间,其遗传距离分别为0.48和0.96 cM.为进一步克隆该基因打下了基础. 相似文献
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利用甲基磺酸乙酯（EMS）诱变粳稻品种兰胜获得了一个能稳定遗传的矮化突变体ddu1。GA3点滴诱导水稻第2叶叶鞘伸长和α 淀粉酶诱导反应表明,ddu1并非为GA的缺陷型和信号传导阻碍矮化。将该突变体与籼稻浙辐802、明恢63和粳稻品种日本晴进行正反交配组,遗传分析表明该突变体受隐性单基因控制,而等位性检测表明ddu1与d1、d18、eui1和eui2均不等位。通过SSR和STS分子标记对F2代分离群体进行遗传定位,将该基因定位于第7染色体SSR标记RM427附近,随后又发展了多对有多态性的SSR和STS分子标记,最终将该基因定位于STS标记R5309和R3742之间,遗传距离分别为0.4和2.0 cM。 相似文献
8.
一个水稻新黄绿叶突变体基因的分子定位 总被引：17，自引：1，他引：16
在水稻品种武运粳7号中发现了一个黄绿叶自然突变体,经过多代自交形成了稳定的突变系。该突变系和武运粳7号的正反交F2代的遗传分析表明该材料的黄绿叶由1对隐性基因控制,命名为 ygl 2。利用已有的微卫星（SSR）标记和新发展的SSR标记将 ygl 2基因定位于RM1340、RM7269、RM6298、SSR6 16和RM7434、SSR6 5、SSR6 9、RM5957之间,排列位置为RM1340-RM7269-RM6298-SSR6 16 -ygl 2-RM7434-SSR6 5、SSR6 9-RM5957,它们之间的遗传距离分别为238、0.37、0.00、0.62、0.74、0.49、0.86和1.62 cM,这为 ygl 2基因的分子标记辅助选择育种和图位克隆奠定了基础。 相似文献
9.
利用甲基磺酸乙酯（EMS）诱变粳稻品种兰胜获得了一个能稳定遗传的矮化突变体ddu1。GA3点滴诱导水稻第2叶叶鞘伸长和α淀粉酶诱导反应表明,ddu1并非为GA的缺陷型和信号传导阻碍矮化。将该突变体与籼稻浙辐802、明恢63和粳稻品种日本晴进行正反交配组,遗传分析表明该突变体受隐性单基因控制,而等位性检测表明ddu1与d1、d18、eui1和eui2均不等位。通过SSR和STS分子标记对F2代分离群体进行遗传定位,将该基因定位于第7染色体SSR标记RM427附近,随后又发展了多对有多态性的SSR和STS分子标记,最终将该基因定位于STS标记R5309和R3742之间,遗传距离分别为0.4和2.0 cM。 相似文献
10.
水稻矮化突变体ddul的遗传分析和分子定位 总被引：4，自引：0，他引：4
利用甲基磺酸乙酯(EMS)诱变粳稻品种兰胜获得了一个能稳定遗传的矮化突变体ddu1.GA3点滴诱导水稻第2叶叶鞘伸长和α-淀粉酶诱导反应表明,ddu1并非为GA的缺陷型和信号传导阻碍矮化.将该突变体与籼稻浙辐802、明恢63和粳稻品种日本晴进行正反交配组,遗传分析表明该突变体受隐性单基因控制,而等位性检测表明ddu1与d1、d18、eui1和eui2均不等位.通过SSR和STS分子标记对F2代分离群体进行遗传定位,将该基因定位于第7染色体SSR标记RM427附近,随后又发展了多对有多态性的SSR和STS分子标记,最终将该基因定位于STS标记R5309和R3742之间,遗传距离分别为0.4和2.0 cM. 相似文献
11.
A short root mutant ksr1 with the Kasalath background was isolated from an EMS-mutagenized population in rice. The root length of 6-day-old ksr1 seedlings was only about 20% of the wild type. Genetic analysis indicated that the short root phenotype of ksr1 was controlled by a recessive mutation in a single nuclear-encoded gene. To map the ksr1 mutation, an F2 population was generated by crossing the ksr1 mutant with Nipponbare. The KSR1 locus was linked to the SSR marker RM1223 on rice chromosome 4. Eight n... 相似文献
12.
Identification and Fine Mapping of a Gene Related to Pale Green Leaf Phenotype near the Centromere Region in Rice （Oryza sativa） 总被引：1，自引：0，他引：1
ZHU Li LiuWenzhen WU Chao LUAN Wei jiang Fu Ya ping Hu Guo cheng SI Hua min SUN Zong xiu 《水稻科学》2007,14(3):172-180
A thermo-insensitive pale green leaf mutant (pgl2) was isolated from T-DNA inserted transgenic lines of rice (Oryza sativa L. subsp. japonica cv. Nipponbare). Genetic analysis indicated that the phenotype was caused by a recessive mutation in a single nuclear-encoded gene. To map the PGL2 gene, an F2 population was constructed by crossing the mutant with Longtefu (Oryza sativa L. subsp. indica). The PGL2 locus was roughly linked to SSR marker RM331 on chromosome 8. To finely map the gene, 14 new InDel markers were developed around the marker, and PGL2 was further mapped to a 2.37 Mb centromeric region. Analysis on chlorophyll contents of leaves showed that there was no obvious difference between the mutant and the wild type in total chlorophyll (Chl) content, while the ratio of Chl a / Chl b in the mutant was only about 1, which was distinctly lower than that in the wild type, suggesting that the PGL2 gene was related to the conversion between Chl a and Chl b. Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres. 相似文献
13.
LI Jin-bo XIA Ming-yuan WAN Bing-liang DU Xue-shu ZHA Zhong-ping YU Da-zhao QI Hua-xiong 《水稻科学》2009,16(1):79-82
A mutant with twisted hulls was found in a breeding population of rice (Oryza sativa L.). The mutant shows less grain weight and inferior grain quality in addition to twisted hulls. Genetic analysis indicated that the phenotype of mutant was controlled by a single recessive gene (temporarily designated as TWH). To map the TWH gene, an F2 population was generated by crossing the twh mutant to R725, an indica rice variety with normal hulls. For bulked segregant analysis, the bulk of mutant plants was prepared by mixing equal amount of plant tissue from 10 twisted-hull plants and the bulk of normal plants was obtained by pooling equal amount tissue of 10 normal-hull plants. Two hundred and seven pairs of simple sequence repeat (SSR) primers, which are distributed on 12 rice chromosomes, were used for polymorphism analysis of the parents and the two bulks. The TWH locus was initially mapped close to the SSR marker RM526 on chromosome 2. Therefore, further mapping was performed using 50 pairs of SSR primers around the marker RM526. The TWH was delimited between the SSR markers RM14128 and RM208 on the long arm of chromosome 2 at the genetic distances of 1.4 cM and 2.7 cM, respectively. These results provide the foundation for further fine mapping, cloning and functional analysis of the TWH gene. 相似文献
14.
Molecular Markers and Candidate Genes for Thermo-Sensitive Genic Male Sterile in Rice 总被引：1，自引：0，他引：1
Sudthana KHLAIMONGKHON Sriprapai CHAKHONKAEN Keasinee PITNGAM Khanittha DITTHAB Numphet SANGARWUT Natjaree PANYAWUT Thiwawan WASINANON Chareerat MONGKOLSIRIWATANA Julapark CHUNWONGSE Amorntip MUANGPROM 《水稻科学》2019,26(3):147-156
The discovery of thermo-sensitive genic male sterility(TGMS) has led to development of a simple and highly efficient two-line breeding system. In this study, genetic analysis was conducted using three F_2 populations derived from crosses between IR68301 S, an indica TGMS rice line, and IR14632(tropical japonica), Supanburi 91062(indica) and IR67966-188-2-2-1(tropical japonica), respectively.Approximately 1:3 ratio between sterile and normal pollen of F_2 plants from the three populations revealed that TGMS is controlled by a single recessive gene. Bulked segregant analysis using simple sequence repeat(SSR) and insertion-deletion(InDel) markers were used to identify markers linked to the tms gene. The linkage analysis based on the three populations indicated that the tms locus was located on chromosome 2 covering the same area. Using IR68301S × IR14632 F_2 population, the results showed that the tms locus was located between SSR marker RM12676 and InDel marker 2gAP0050058. The genetic distance from the tms gene to these two flanking markers were 1.10 and 0.82 cM, respectively.InDel marker 2gAP004045 located between these two markers showed complete co-segregation with the TGMS phenotype. In addition, InDel marker vf0206114052 showed 2.94 cM linked to the tms gene using F_2 populations of IR68301S × Supanburi 91062. These markers are useful tool for developing new TGMS lines by marker-assisted selection. There were ten genes located between the two flanking markers RM12676 and 2gAP0050058. Using quantitative real-time PCR for expression analysis, 7 of the 10 genes showed expression in panicles, and response to temperatures. These genes could be the candidate gene controlling TGMS in IR68301S. 相似文献
15.
水稻淡褐斑叶突变体lbsl1的遗传分析与基因定位 总被引：1，自引：0，他引：1
通过EMS诱变籼稻品种IR64获得一个稳定遗传的淡褐色斑点叶突变体lbsl1（light brown spotted leaf 1）。在自然条件下,突变体播种后10~14 d,叶片上出现淡褐色斑点,随后逐渐扩散至全叶,第1叶至剑叶上均有淡褐色斑,为全生育期性状。斑点性状的表达对株高、生育期、结实率和千粒重等农艺性状具有显著的影响。遗传分析结果表明,该淡褐色斑点叶性状受一个隐性核基因控制。将突变体lbsl1与正常叶色水稻Morobereken杂交构建F2定位群体,利用SSR标记,最终将该淡褐叶基因lbsl1(t)定位在第6染色体短臂上一个约130 kb的区段上。定位的结果和发展的群体为该基因的进一步精细定位和克隆奠定了基础。 相似文献
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在自然光条件下水培籼稻品种Nankinkodo中,发现根为红色的自然突变体,命名为HG1。水培条件下该突变体在光照强度大于29 μmol/（m2·s）的可见光下,根开始转红,在光照强度为180 μmol/（m2·s）的可见光下,呈鲜红,具有明显的光照敏感性。遗传分析表明,该突变体光照敏感性的红根性状由1对显性基因控制, 暂命名为Lsr。利用微卫星标记将Lsr基因定位在第4染色体上RM252与RM303之间,遗传距离分别为9.8 cM和6.4 cM, 这为Lsr基因的精细定位和克隆奠定了基础。 相似文献
18.
FENG Bao-hua YANG Yang SHI Yong-feng LIN Lu CHEN Jie WEI Yan-lin Hei LEUNG WU Jian-li 《水稻科学》2013,20(1):13-18
A light brown spotted-leaf mutant of rice was isolated from an ethane methyl sulfonate (EMS)- induced IR64 mutant bank. The mutant, designated as lbsl1 (light brown spotted-leaf 1), displayed light brown spot in the whole growth period from the first leaf to the flag leaf under natural summer field conditions. Agronomic traits including plant height, growth duration, number of filled grains per panicle, seed-setting rate and 1000-grain weight of the mutant were significantly affected. Genetic analysis showed that the mutation was controlled by a single recessive gene, tentatively named lbsl1(t), which was mapped to the short arm of chromosome 6. By developing simple sequence repeat (SSR) markers, the gene was finally delimited to an interval of 130 kb between markers RM586 and RM588. The lbsl1(t) gene is likely a novel rice spotted-leaf gene since no other similar genes have been identified near the chromosomal region. The genetic data and recombination populations provided will facilitate further fine-mapping and cloning of the gene. 相似文献