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木薯渣固态发酵植酸酶的条件研究 总被引：5，自引：0，他引：5
木薯渣粗蛋白及无机盐含量较低,与麸皮、米糠混合或同时添加无机氮源及无机盐,可作为发酵培养基合成植酸酶。高浓度的磷抑制植酸酶的合成。最佳发酵条件为：木薯渣为唯一碳原,添加 ２％ ＮＨ４ＮＯ３, ０．０１％ Ｋ２ＨＰＯ４, ０．０５％ ＭｇＳＯ４·７Ｈ２Ｏ, ０．０５％ ＫＣＩ, ０．０１％ ＦｅＳ－Ｏ４· ７Ｈ２Ｏ, ０．９％ ＳＤＳ,培养基水分含量 ６０％～６５％, １０ｇ木薯渣／２５０ ｍＬ三角瓶,接种量为２ｍＬ菌悬液（２．８×１０个孢子／ｍＬ）,自然 ｐＨ值,培养温度 ２８～３１℃,第８ｄ达产酶高峰,高峰期产酶量为 ６７３０ Ｕ／ｇ干曲。 相似文献
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喷族５％安福１８００ｍｌ／ｈｍ＾２、１２００ｍｌ／ｈｍ＾２悬剂液和２５％敌力脱乳油１５００倍液，防治芒果炭疽病均有明显的防治效果，其防效分别为９８。９％、９７．７％和９５．６％。 相似文献
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橡胶树炭疽病大田化学防治试验 总被引：1，自引：0，他引：1
经红明、长征等６个场、所的连续５ａ１７万ｈｍ＾２的橡胶树炭疽病大田防治结果表明：应用２８％复方多菌灵Ｆａｉ６３０ｍＬ／ｈｍ＾２次，兑水７５Ｌ喷雾和１０％百菌清Ｏａｉ１５０ｍＬ／ｈｍ＾２次喷烟雾，平地记效分别为７９．３％和７１．３％。经过防治，挽回干胶损失１９９６．６２ｔ，获得新增产值２３５９．９４万元的经济效益。 相似文献
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Ca2+对水分胁迫条件下芒果叶片膜脂过氧化及膜保护系统的影响 总被引：3，自引：0，他引：3
以芒果幼苗为材料,研究Ｃａ２＋对水分胁迫条件下,叶片膜脂过氧化和膜保护系统的效应。结果表明：与对照叶片相比,涂施Ｃａ２＋后,叶片含水量、超氧化物歧化酶（ＳＯＤ）活性及Ｖｃ含量下降明显减慢;过氧化物酶（ＰＯＤ）活性上升加快且幅度加大,而下降延迟;丙二醛（ＭＤＡ）积累显著减少。Ｃａ２＋专一性螯合剂ＥＧＴＡ可削弱Ｃａ２＋的效应。ＣａＭ 拮抗剂氯丙嗪（ＣＰＺ）对叶片ＳＯＤ活性和ＰＯＤ活性有抑制作用,推测Ｃａ２＋可能通过与ＣａＭ结合调节相关酶活性,从而减轻水分胁迫对膜的伤害。 相似文献
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50％咪鲜胺锰盐可湿粉对芒果炭疽病的防治效果 总被引：1，自引：0，他引：1
在芒果花芽期后喷施4次,第3次喷药后15d,50％咪鲜胺锰盐可湿粉1000倍、1500倍的效果较好,防治效果分别为71．9％、59．0％,显著优于50％咪鲜胺可湿粉1000倍（46．4％）;第4次喷药后15d,50％咪鲜胺锰盐可湿粉1000倍的防治效果为64．2％,显著优于50％咪鲜胺可湿粉1000倍（52．9％）。表明50％咪鲜胺锰盐可湿粉是一种可防治芒果炭疽病的安全而有效的药剂。 相似文献
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田间药效试验结果表明,草甘膦片剂的防效和耐雨性均优于水剂,在睛天或降雨（０．５Ｌ／ｍ２）时用２２５０￣３０００ｇ／ｈｍ２（有效成分）,喷后３０ｄ对白茅等杂草的防效达８８％￣９８％;喷后９０ｄ对白茅效达９５％￣９９．８（株数）和９６％￣９９．８％（鲜重）,对总禾本科杂草防效达９７％￣９９．６％（鲜重）。 相似文献
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为评价复配药剂37%多菌灵·氟硅唑可湿性粉剂防治贡柑炭疽病的效果及其对贡柑的安全性,连续两年进行了37%多菌灵·氟硅唑可湿性粉剂防治贡柑炭疽病的田间药效试验。试验结果表明,37%多菌灵·氟硅唑可湿性粉剂有效成分用量148、185和246.7 mg/L对贡柑炭疽病有较好的防治效果,施药3次后10 d调查,2014年的防治效果分别为71.90%、79.48%和90.16%,2015年分别为65.09%、76.18%和87.36%,该药剂值得在生产上推广应用。 相似文献
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笔花豆炭疽病大田防治试验研究 总被引：1，自引：0，他引：1
格拉姆笔花豆炭疽病在海南危害严重。大田化学防治试验表明,应用20％多菌灵乳剂,有效成分450g/hm~2·次,在作物生长期和花期,连续喷药3次,可获得满意的防治效果,产量增加36％～60.8％。 相似文献
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6年的研究结果表明,用苯莱特、复方多菌灵和拌种双,在芒果花期喷药6次,果实发育期喷药2～3次,可提高座果率,增加产量,并可在一定程度上减少真菌潜伏侵染数量,减轻果实采后腐烂的严重程度,提高果实贮藏效果。 相似文献
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Anthracnose disease of papaya, caused by Colletotrichum gloeosporioides Penz, can cause extensive postharvest losses. The goal of this research was to use pre-harvest calcium applications to reduce anthracnose disease. Six pre-harvest foliar calcium sprays were applied biweekly to papaya trees in experimental orchards at Universiti Putra Malaysia. Additional in vitro and in vivo tests were carried out to test the effect of calcium on fruit calcium content, spore germination, mycelial growth and disease severity. Calcium chloride at 1%, 1.5% and 2.0% concentrations significantly decreased spore germination. Calcium content of papaya fruit was significantly increased by calcium sprays at a concentration of 2.0% in 2012 and 2013. In vivo studies showed that increasing calcium content in fruit by calcium sprays at 1.5 and 2.0% concentrations significantly reduced anthracnose incidence of fruits during five weeks storage at 12 ± 2 °C, and delayed initiation of disease symptoms by four weeks. 相似文献
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A range of fungicides were evaluated for control of anthracnose (Colletotrichum lupini) in lupin. Glasshouse investigation identified fungicides that reduced disease severity with varying degrees of efficacy. When applied 1 d prior to infection, azoxystrobin, chlorothalonil, mancozeb and copper oxychloride fungicides were highly effective. Systemic fungicides such as tebuconazole, benomyl and carbendazim were less effective. Application timing was important; fungicides such as chlorothalonil and mancozeb were less effective when applied 5 d prior to infection compared with 1 d prior. Application after infection was ineffective for all fungicides. In field experiments application of azoxystrobin, mancozeb or chlorothalonil during flowering and podding reduced incidence of anthracnose infection on pods. Yield responses occurred in moderately susceptible, moderately resistant or resistant cultivars. Seed infection was reduced but not eradicated. Application of foliar fungicides for anthracnose control is potentially a viable management option for lupin production in high anthracnose risk areas of Western Australia. 相似文献
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香蕉组培苗果实的采后病害及防腐保鲜 总被引：3，自引：0，他引：3
对香蕉青果和贮藏期间的病果进行了分离培养和致病性测定,鉴定出潜伏侵染幼果的真菌有Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍｍｕｓａｅ（Ｂｅｒｋ＆Ｃｕｒｔ．）Ａｒｘ,Ｆｕｓａｒｉｕｍｓｐｐ．Ｐｅｔａｌｏｔｉａｓｐ．和Ｍａｒｃｒｏｐｈｏｍａｍｕｓａｅ等,造成贮藏病害的主要病菌有Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍｍｕｓａｅ,Ｆｕｓａｒｉｕｍｓｐｐ;Ｖｅｒｔｉｃｉｌｉｕｍｓｐ;Ｍａｃｒｏｐｈｏｍａｍｕｓａｅ;Ｂｏｔｒｙｏｄｉ 相似文献
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Anthracnose is a postharvest disease of banana caused by the fungus Colletotrichum musae that results in major economic losses during transportation and storage. For the management of banana anthracnose, antifungal effects of Arabic gum (AG) (5, 10, 15 and 20%), chitosan (CH) (1.0%), and the combination of AG with CH were investigated in vitro as well as in vivo. CH at 1.0% and 1.5% had fungicidal effects on C. musae. AG alone did not show any fungicidal effects while the combination of 1.0% CH with all tested AG concentrations had fungicidal effects. However, the potato dextrose agar (PDA) medium amended with 10% AG incorporated with 1.0% CH showed the most promising results among all treatments in suppressing the mycelial growth (100%) and conidial germination inhibition (92.5%). In vivo analysis also revealed that 10% AG incorporated with 1.0% CH was the optimal concentration in controlling decay (80%), showing a synergistic effect in the reduction of C. musae in artificially inoculated bananas. The 10% AG incorporated with 1.0% CH coatings significantly delayed ripening as in terms of percentage weight loss, fruit firmness, soluble solids concentration and titratable acidity. The results showed the possibility of using 10% Arabic gum incorporated with 1.0% chitosan as a biofungicide for controlling postharvest anthracnose in banana. 相似文献
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天然橡胶是四大工业原料中唯一的可再生资源,由炭疽病菌侵染引起的橡胶树炭疽病是当前我国橡胶生产上最为严重的两大叶部病害之一。本研究以分离自云南保山的喀斯特炭疽病菌MeCkYN1705为对照,对海南新发炭疽病菌喀斯特炭疽菌HCkHNQZ1736进行抗药性评价。结果表明,菌株HCkHNQZ1736对苯并咪唑类杀菌剂多菌灵表现出高抗药性,EC50达1107.2654 µg/mL,而MeCkYN1705的EC50仅为0.0554 µg/mL。克隆了菌株HCkHNQZ1736的tub2基因,序列分析发现其所编码的第198个氨基酸位点由谷氨酸（Glu-E）突变为丙氨酸（Ala-A）,推测该氨基酸位点突变是导致该菌株产生多菌灵抗性的原因。此外生物学特性测定发现,该菌株最适生长温度为28 ℃,致死温度为35 ℃;最适生长pH为6;光暗交替利于菌落生长;果胶、蛋白胨分别为最适碳源和氮源。 相似文献