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1.
中国明对虾C 型凝集素基因(Fclectin)的重组表达及活性分析 总被引：1，自引：1，他引：0
研究拟通过分析对虾C型凝集素的活性特点,探讨其在对虾先天免疫应答过程中的潜在功能以及在养殖生产实践中的应用。实验利用原核表达系统对中国明对虾C-型凝集素基因的两个串联的糖识别结构域(carbohydrate recognition domain,CRD)进行了重组表达,并通过纯化复性获得了重组目的蛋白(rFclectin-CRD1和rFclectin-CRD2)。活性分析结果显示,重组目的蛋白对多种病原菌有凝集和抑制生长的作用,并且具有Ca2+依赖活性;其凝集活性可被半乳糖、肽聚糖、脂多糖等多种病原相关分子模式所抑制,研究结果证实,Fclectin是一种典型的C-型凝集素,它可能作为中国明对虾先天免疫中重要的模式识别受体,在一定程度上参与了机体应答病原微生物的防御过程。 相似文献
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为有效地进行凡纳滨对虾病害防治和健康养殖,探究对虾天然免疫机制,为其免疫防控提供新思路,实验根据本课题组前期转录组结果提示,在凡纳滨对虾血细胞中克隆获得了一个新的C型凝集素基因(LvLc2, GenBank登录号KR020738)。生物信息学分析显示,LvLc2的ORF区全长465 bp,编码154个氨基酸(aa),5′-UTR为11 bp,3′-UTR为126 bp;其预测的分子量为17.16 ku,理论等电点为4.54;推断其氨基端含有一个由17个aa组成的信号肽序列,羧基端含有一个保守的糖识别结构域(CRD)。该基因在凡纳滨对虾各种组织中有不同程度的表达,其中在肠道中表达量最高,其次为胃、肝胰腺、血细胞等组织或细胞,在肌肉中表达量最低。利用脂多糖(LPS)和肽聚糖(PGN)刺激对虾后,比较分析了LvLc2的表达变化特征,不同类型的病原表面多糖可在刺激后不同时期引发LvLc2基因的应答改变,表明其具有潜在的广谱应答模式,同时针对不同病原又具有不同的表达特征,显示了其识别结合和间接免疫效应的差异。对其作用特征的探讨有助于更好地了解对虾C型凝集素在病原感染过程中的功能和作用机制。研究结果为深入探讨对虾天然免疫调控机制提供了参考,在对虾健康养殖及病害防治方面具有潜在的应用价值。 相似文献
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C型凝集素(C-type lectin)是一类能与糖类结合的非抗体的蛋白质或糖蛋白家族,为了研究C型凝集素基因在日本沼虾组织分布、细胞定位和细菌感染过程中的表达情况,本研究应用cDNA末端快速克隆(rapid-amplification of cDNA ends,RACE)技术首次克隆了日本沼虾C型凝集素结构域家族3基因(MnLec3)的全长序列,通过实时荧光定量PCR(qRT-PCR)分析MnLec3基因在不同组织、细菌感染后不同时间的表达水平,Western blot和免疫荧光分别分析蛋白的表达水平和细胞定位。结果显示,MnLec3基因cDNA全长1 357 bp,包括125 bp的5′末端非翻译区(UTR)、1 026 bp的开放阅读框(ORF)和206 bp的3′UTR,其中开放阅读框编码341个氨基酸。氨基酸序列比对显示,日本沼虾MnLec3基因含有保守钙结合点(Met 1-Glu17)和糖识别结构域(CRD)。同源性分析结果显示,MnLec3与罗氏沼虾C型凝集素3相似度较高;邻接法(Neighbor-Joining,NJ)进化树分析结果显示,MnLec3与其他甲壳动物C型凝集素聚为一支。通过构建原核表达载体获得体外重组蛋白rMnLec3,并将纯化重组蛋白免疫大鼠获得抗血清,免疫荧光结果显示,绿色荧光信号主要在肝胰腺细胞核中表达。qRT-PCR结果显示,MnLec3在日本沼虾所检测组织中均表达,其中肝胰腺中表达量最高,血细胞次之;与对照组相比,在嗜水气单胞菌刺激12～48 h时MnLec3表达量显著升高,48 h表达量最高,Western blot分析结果显示,MnLec3蛋白表达丰度与基因表达模式基本相似,提示克隆得到的MnLec3参与日本沼虾抵御细菌入侵的免疫过程。 相似文献
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本文利用原核表达系统对凡纳滨对虾C-型凝集素基因的开放阅读框进行了重组表达,并通过纯化分离获得重组目的蛋白,采用血细胞悬浮培养的方法,研究了重组C-型凝集素对凡纳滨对虾血细胞免疫作用的影响。实验梯度设置为：对照组和重组C-型凝集素蛋白（1.0 mg/mL）,分别在0、3、6、9、12、24h取培养的血细胞和培养液进行免疫相关指标的测定。结果表明：重组C-型凝集素经SDS-PAGE分析显示在39KD处有显著诱导条带,与预测的分子量大小基本一致,Western blot分析可以与anti-His抗体特异性结合,从而证明C-型凝集素重组蛋白在大肠杆菌中成功表达。重组C-型凝集素对大肠杆菌具有明显的凝集作用,对凡纳滨对虾血细胞数量、存活率和吞噬率影响显著（P<0.05）;培养基酚氧化酶活力在培养3-12h内呈峰值变化,于9h时达到最大值（P<0.05）。研究表明,C-型凝集素不仅具有凝集作用,而且还能激活酚氧化酶原系统,具有重要的免疫防御作用。 相似文献
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C型凝集素受体（C-type lectin receptor，CTLR）是可以特异性结合糖类病原体相关分子模式（pathogen-associated molecular patterns，PAMPs）的模式识别受体（pattern recognition receptors，PPRs），在先天性免疫中发挥着重要作用。为了揭示硬骨鱼CTLR的生物学功能，本研究以从大黄鱼（Larimichthys crocea）转录组数据库中筛选出的一个CTLR基因—C型凝集素结构域家族4成员E基因（C-type lectin domain family 4 member E gene，Clec4e）为研究对象，研究其分子特征、表达分布和凝集特性。结果显示，LcClec4e cDNA全长1546 bp，开放阅读框（open reading frame，ORF）771 bp，编码254个氨基酸。LcClec4e的N端有一个跨膜区，无信号肽，C端含有一个糖识别结构域（carbohydrate recognition domain，CRD），其中含有糖结合位点EPN和WFD以及6个可形成二硫键的保守半胱氨酸。系统发育分析表明，LcClec4e与多种鲈形目鱼类Clec4e具有较近的亲缘关系。荧光定量PCR结果显示，LcClec4e在所检测的10种组织中呈组成型分布，且在肝脏中表达量最高；LcClec4e在来源于大黄鱼头肾组织的原代巨噬细胞、淋巴细胞和粒细胞中均有表达，且在巨噬细胞中表达量最高；经灭活溶藻弧菌刺激后，LcClec4e在3种免疫细胞中的表达均极显著上调。原核表达的重组LcClec4e胞外段（recombinant LcClec4e-extracellular domain，rLcClec4e-ex）具有Ca2+依赖性的凝集活性，可凝集小鼠、家兔的红细胞，以及嗜水气单胞菌、变形假单胞菌、溶藻弧菌和坎氏弧菌等4种水产常见的革兰氏阴性菌。D-葡萄糖、D-果糖、D-甘露糖、D-麦芽糖、α-乳糖和脂多糖均可抑制rLcClec4e-ex对大黄鱼重要病原菌变形假单胞菌的凝集作用，说明LcClec4e可能与变形假单胞菌表面的糖类物质结合。这些研究结果提示，LcClec4e可能作为一种PPR，通过结合病原菌表面的糖类PAMPs来识别病原，参与大黄鱼抗细菌感染的免疫防御。 相似文献
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通过RT-PCR扩增三疣梭子蟹C-型凝集素（C-type lectin like-domain protein,CTLD）基因序列,将目的基因克隆入质粒pET32a,构建原核表达重组质粒pET32a-CTLD.将重组质粒转化入大肠杆菌BL21中,通过IPTG诱导CTLD的表达,对其表达产物进行SDS-PAGE和Western blot 检测.用His GraviTrap Ni亲和层析柱及超滤离心管纯化目的蛋白,并对其进行活性检验.结果表明,构建得到CTLD基因的原核表达载体,双酶切鉴定得到与预期片段相符的外源基因插入片段,经测序与目的序列完全一致;诱导表达出相对分子质量（M） 39 600的目的蛋白,经SDS-PAGE和Western blot检测,融合蛋白成功表达;重组C-型凝集素对兔的红细胞没有明显的凝集作用,但对小鼠红细胞的凝集效价是22,而对所选5种细菌的凝集作用存在差异,其中对大肠杆菌具有明显的凝集作用,而对溶藻弧菌、哈维氏弧菌、鳗弧菌和金黄色葡萄球菌均无凝集现象.本研究结果为进一步研究C-型凝集素在免疫防御中的作用与地位提供依据. 相似文献
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中国对虾血细胞凝集素的性能研究↑（*） 总被引：15，自引：0，他引：15
对中国对虾(Penaeuschinensis)的血细胞凝集素部分性能的研究结果表明,中国对虾的血淋巴中存在可凝集多种脊椎动物血细胞的因子—血细胞凝集素,凝集活力的大小同中国对虾的健康状况有密切关系,发病对虾体内的凝集素活力明显下降。该凝集素具有较强的热稳定性,100℃处理20min仍具活性;并且具有广泛的pH作用范围,可在pH3～11产生凝集作用;适当增加盐度(18～36)可以提高该凝集素的凝集活性;其活性还会被EDTA强烈抑制;但对Ca2 、Mg2 、Mn2 等离子和多种糖类不敏感。在中国对虾血细胞的细胞膜上未发现有凝集鸡红细胞的活性物质。 相似文献
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通过cDNA末端快速扩增(Rapid amplification of c DNA ends,RACE)技术克隆得到魁蚶(Scapharca broughtonii)C型凝集素(C-type lectin,Sb-Lec1)基因,该基因全长为700 bp,其中,5′-UTR为29 bp,3′-UTR为167 bp,开放阅读框长度为504 bp,编码167个氨基酸,包括长度为23个氨基酸的信号肽序列、129个氨基酸的糖识别结构域(CRD)以及参与二硫键形成的6个半胱氨酸。预测蛋白分子量为19.11 k Da,理论等电点为4.74。多序列比对结果显示,Sb-Lec1基因CRD编码的氨基酸序列与长牡蛎(Crassostrea gigas)、紫贻贝(Mytilus galloprovincialis)和海湾扇贝(Argopecten irradians)C型凝集素的同源性分别为38%~40%、34%~35%和38%~39%,Sb-Lec1基因编码的氨基酸序列与其他物种的凝集素基因具有相似的结构,均含有形成二硫键的4个保守半胱氨酸。系统进化分析结果显示,魁蚶先与贝类聚为一支,再与脊椎动物聚在一起,表明魁蚶Sb-Lec1基因在进化树上的位置与其传统分类所处位置一致。采用荧光定量PCR技术,检测了Sb-Lec1基因在组织中的表达情况,发现其在肝胰腺、血淋巴、鳃、外套膜、闭壳肌、斧足中均有表达,其中,肝胰腺表达量最高。同时,分析了Sb-Lec1基因在鳗弧菌(Vibrio anguillarum)刺激下的mRNA表达量变化情况。结果显示,与对照组相比,菌刺激组Sb-Lec1基因mRNA在各检测组织中的表达量均显著上调(P0.05),随着刺激时间的延长,表达量呈先升高后降低的趋势。本研究表明,魁蚶Sb-Lec1基因在机体免疫防御方面发挥重要功能。 相似文献
9.
relish是Rel/NF-κB家族的重要成员之一。本研究在构建的斑节对虾(Penaeus monodon)肝胰腺转录组文库中克隆得到PmRelish基因,其全长5 112 bp,包括3 561 bp的ORF,推测编码1个含1 186个氨基酸的蛋白。PmRelish的蛋白具有Rel/NF-κB蛋白家族的典型结构特征,包括N端的RHD结构域,核定位信号,C端的6个锚蛋白重复序列以及死亡结构域,表明该蛋白是NF-κB1亚型。该基因在所检测组织中呈组成型表达,并在血细胞表达量最高。使用定量PCR检测PmRelish基因在不同免疫刺激条件下在肝胰腺中的mRNA表达情况,结果显示,肝胰腺PmRelish基因是诱导型表达,并被创伤弧菌、金黄色葡萄球菌和白斑综合征病毒(WSSV)调控表达,其中被创伤弧菌上调表达最显著。进一步证明了病毒核酸类似物Poly(I:C)、R484可激活PmRelish的表达。上述研究结果说明Relish是斑节对虾的重要免疫因子,可能通过直接或间接作用参与对细菌和病毒的免疫反应。 相似文献
10.
秀丽白虾4种C型凝集素基因的克隆、组织表达与进化分析 总被引：1，自引：1，他引：0
C型凝集素是一类Ca2 依赖活性的糖蛋白,在虾蟹类的免疫防御中发挥着重要作用。采用RT-PCR及RACE技术首次克隆了秀丽白虾C型凝集素家族4个基因( EmCTL-1、EmCTL-2、EmCTL-3、EmCTL-4)的cDNA全长。这4个C型凝集素基因的cDNA全长分别为1 390、1 119、1 364和1 299 bp,分别含996、969、 1 026和1 044 bp的开放阅读框,各编码332、322、341和347个氨基酸的蛋白。其氨基酸序列与罗氏沼虾相应的C型凝集素的相似性分别为40%、37%、69%和73%, 与其他海水虾类的相似性在30%～41%之间。阳性选择模型分析显示虾类的C型凝集素基因在进化过程中经历了阳性选择。进化树分析表明,秀丽白虾的 C型凝集素基因与罗氏沼虾的亲缘关系最近,并且除EmCTL-4属于十足类C型凝集素亚群A外,其他均属于亚群C。组织表达分析表明,EmCTL-1主要在血液中表 达,EmCTL-2主要在性腺、血液和胸神经节中表达,而EmCTL-3和EmCTL-4则主要在精巢中表达。研究表明,秀丽白虾的C型凝集素基因具有较快的 进化特点,同时又具备凝集素行使免疫功能时必需的保守氨基酸残基及钙离子结合位点,精巢和血液是秀丽白虾C型凝集素基因主要的表达器官。 相似文献
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七种不同结构糖源对凡纳滨对虾三大营养物质代谢的影响 总被引：4，自引：2，他引：2
本实验研究了不同结构糖源饲料对初始体质量为(0.14±0.01)g的凡纳滨对虾生长、体组成及相关代谢的影响。实验共设7个处理,基础饲料中分别添加7种结构不同的糖源:葡萄糖、果糖、葡萄糖+果糖(1∶1)、蔗糖、麦芽糖、玉米淀粉和糊精,添加水平为20%。每处理设3个平行,每个平行放50尾虾,实验期56 d。结果表明,实验饲料对凡纳滨对虾的特定生长率和蛋白质效率有显著影响,呈现多糖蔗糖其他,其中葡萄糖组最低;实验饲料对凡纳滨对虾的全虾水分、灰分、蛋白质无显著影响,但对脂肪、肝糖原、肌糖原的影响显著。不同结构的糖源饲料对糖代谢相关酶及产物产生显著影响,对脂肪和蛋白代谢酶及产物影响不显著。 相似文献
13.
C Rosas G Cuzon G Taboada C Pascual G Gaxiola A Van Wormhoudt 《Aquaculture Research》2001,32(7):531-547
The influence of protein and energy levels on growth rate, survival, pre- and post-prandial oxygen consumption, ammonia excretion, haemolymph glucose (HG), glycogen in digestive gland and osmotic pressure (OP) in white shrimp Litopenaeus vannamei (Boone) and L. setiferus (Linne) juveniles was studied. Diets containing a high-quality protein at a protein/energy (P/E) ratio of 16, 26, 31 and 36 mg kJ−1 were fed at 20% of shrimp body weight of two sizes: < 1 g and > 1 g. Both species showed a optimum P/E ratio of 36 mg kJ−1 (33–44% protein and 6–23% carbohydrate) in juveniles < 1 g. For shrimp > 1 g, L. setiferus showed a higher growth rate in the diet with 16 mg kJ−1 (27% protein; 32% carbohydrate) and L. vannamei between 26 and 36 mg kJ−1 (33–44% protein and 6–23% carbohydrate). In both experiments, the growth rate of L. vannamei was 2–3 times that observed in L. setiferus. Routine oxygen consumption and apparent heat increment (AHI) of L. setiferus juveniles was two times higher than that observed in L. vannamei juveniles, which could indicate that L. setiferus has a higher metabolic rate. The O/N ratio varied according to protein level, with higher values (O/N = 180) with a 16-mg kJ−1 diet and lower values (O/N = 73) with a 36-mg kJ−1 diet in L. setiferus juveniles. A similar variation in O/N ratio was obtained in L. vannamei fed with all diets with an interval between 22 and 50. An inverse relation between ammonia excretion and HG, and digestive gland glycogen (DGG) in relation to an increase in the P/E ratio indicate that both shrimp species are well adapted to use carbohydrates and/or proteins from their diet. The higher values of hyper-osmotic capacity (hyper-OC) were observed in L. setiferus < 1 g fed with 36 mg kJ−1 and the lowest in L. vannamei < 1 g fed with 31 mg kJ−1. Intermediate values of hyper-OC were observed in both species fed all diets indicating that osmotic factors of juveniles < 1 g of both species are more affected by the P/E ratio than juveniles > 1 g. All results showed that juveniles > 1 g of both species are less dependent of P/E ratio than juveniles < 1 g. Litopenaeus vannamei is a most tolerant shrimp species with a high capacity to use a wide range of dietary P/E ratios for growth, which may be due to its lower energy requirements. Litopenaeus setiferus showed a lower capacity to accept different P/E ratios but the optimum P/E ratio obtained with this species shows that L. setiferus accept diets with a high carbohydrate level as well. These results demonstrate that there are nutritional and physiological differences that explain the differences that have been observed when both species were cultured in commercial ponds. 相似文献
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A 40‐day experiment was conducted to investigate the effects of different Ca2+ concentration fluctuation on the moulting, growth and energy budget of juvenile Litopenaeus vannamei with an initial wet body weight of (1.20±0.01) g. The Ca2+ concentration of the control group C385 was 385 mg L?1 throughout the experiment, while the Ca2+ concentration of the C591, C803, C1155 and C2380 groups periodically fluctuated from 385 to 591, 803, 1155 and 2380 mg L?1 respectively. The moulting frequency (MF) of the shrimp in the Ca2+ concentration fluctuating groups was significantly higher than those in the control group (P<0.05). The specific growth rates (SGRd) and feed intake of the shrimp in the C591 and C803 groups were significantly higher than those in other groups (P<0.05), but no significant differences in feed efficiency were found among all groups (P>0.05). The shrimp in C591 and C803 groups spent significantly less energy in respiration, while depositing significantly more energy for growth than those in other three groups (P<0.05). These results showed that proper Ca2+ concentration fluctuation could increase the MF and growth rate of the juvenile L. vannamei, and according to the regression formula made using SGRd and range of Ca2+ concentration fluctuation, periodically enhanced Ca2+ concentration of 295 mg L?1 in the seawater was suggested to be used in shrimp culture. 相似文献
15.
A new mannose-binding lectin was purified from plasma of freshwater fish, rohu (Labeo rohita) by ammonium sulphate precipitation followed by DEAE-cellulose ion-exchange chromatography. The hemagglutinating activity
is strong for neuraminidase-treated rabbit erythrocytes. Mannose, glucose and their derivatives inhibit hemagglutination.
The apparent molecular weight was determined to be 210 kDa in native PAGE. Analysed on SDS-PAGE under reducing and non-reducing
conditions, the protein migrated as a single band of mol.wt. 40,000. The lectin is acidic in nature showing a pI of 4.5. It
is a glycoprotein containing complex glycan part as indicated by carbohydrate analysis using high-pressure anion exchange
chromatography with a pulsed amperometric detector. The N-terminal sequence (WLNGIGTQIPMKITT) shows no significant homology
with known proteins. It appears to be a C-type lectin as Ca2+ is essential for carbohydrate-binding activity. Methyl -α- D-mannopyranoside was found to be the most potent inhibitor among
the monosaccharides and disaccharides tested. Purified lectin was found to induce intracellular superoxide production following
opsonization of E. coli by its own macrophages.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
16.
Ingestion of food pellets containing Escherichia coli overexpressing the heat‐shock protein DnaK protects Penaeus vannamei (Boone) against Vibrio harveyi (Baumann) infection 
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下载免费PDF全文 S Sinnasamy N Mat Noordin T H MacRae M Ikhwanuddin bin Abdullah P Bossier M E bin Abdul Wahid A Noriaki Y Y Sung 《Journal of fish diseases》2016,39(5):577-584
Feeding aquatic animals with bacterial encapsulated heat‐shock proteins (Hsps) is potentially a new method to combat vibriosis, an important disease affecting aquatic animals used in aquaculture. Food pellets comprised of shrimp and containing Escherichia coli overexpressing either DnaK‐DnaJ‐GrpE, the prokaryotic equivalents of Hsp70‐Hsp40‐Hsp20, or only DnaK were fed to juveniles of the white leg shrimp Penaeus vannamei, and protection against pathogenic Vibrio harveyi was determined. Maintaining pellets at different temperatures for varying lengths of time reduced the number of live adhering E. coli, as did contact with sea water, demonstrating that storage and immersion adversely affected bacterial survival and attachment to pellets. Feeding P. vannamei with E. coli did not compromise their survival, indicating that the bacteria were not pathogenic to shrimp. Feeding P. vannamei with pellets containing bacteria overproducing DnaK (approximately 60 cells g?1 pellets) boosted P. vannamei survival twofold against V. harveyi, suggesting that DnaK plays a role in Vibrio tolerance. Pellets containing DnaK were effective in providing protection to P. vannamei for up to 2 weeks before loss of viability and that DnaK encapsulated by these bacteria enhanced shrimp resistance against Vibrio infection. 相似文献
17.
David D. Kuhn Gregory D. Boardman Steven R. Craig George J. Flick Jr. Ewen McLean 《Journal of the World Aquaculture Society》2007,38(1):74-84
Reuse of fish effluent for the culture of marine shrimp, such as Pacific white shrimp, Litopenaeus vannamei, could provide an opportunity for the US shrimp farming industry to ease constraints (e.g., environmental concerns and high production costs) that have limited them in the past. In this study under laboratory‐scale conditions, the feasibility of culturing L. vannamei in effluents derived from a commercial facility raising tilapia in recirculating aquaculture systems (RAS), supplemented with various salt combinations, was compared to the shrimp’s survival and growth in well water supplemented with 17.6 (control) and 0.6 (freshwater treatment) g/L synthetic sea salt. Three independent trials were conducted in RAS in which survival and growth in the control, the freshwater treatment, and two effluent treatments were compared. Water quality during this study was within safe levels and no differences (P < 0.05) between treatments were observed for dissolved oxygen, nitrite, pH, total ammonia nitrogen, and temperature. However, average nitrate and orthophosphate levels were consistently more than an order of magnitude greater in the effluent treatments compared to the control and the freshwater treatments. Survival and growth of shrimp over 6‐wk periods did not vary significantly between the control and the freshwater treatments; however, shrimp tested in the tilapia effluents often exhibited significant effects (P < 0.05) depending on the salts added. In the low‐salinity waters, correlations (P < 0.05) were observed between Ca2+, Mg2+, Ca2+ and Mg2+, K+, Na+ : K+ and Ca2+ : K+, and shrimp survival and growth. The results of this study revealed that L. vannamei can be raised in tilapia effluent when supplemented with synthetic sea salt (0.6 g/L), CaO (50 mg/L Ca2+), and MgSO4 (30 mg/L Mg2+). 相似文献
18.
Immune responses of whiteleg shrimp,Litopenaeus vannamei (Boone, 1931), to bacterially expressed dsRNA specific to VP28 gene of white spot syndrome virus 下载免费PDF全文
下载免费PDF全文 G Taju N Madan S Abdul Majeed T Raj Kumar S Thamizhvanan S K Otta A S Sahul Hameed 《Journal of fish diseases》2015,38(5):451-465
In this study, dsRNA specific to VP28 gene of white spot syndrome virus (WSSV) of shrimp was synthesized in Escherichia coli in large scale and studied the immune response of shrimp to dsRNA‐VP28. The haematological parameters such as clotting time and total haemocytes counts, and immunological parameters such as prophenoloxidase (proPO), superoxide dismutase (SOD), superoxide anion (SOA) and malondialdehyde content, as well as the mRNA expression of ten immune‐related genes were examined to estimate the effect of dsRNA‐VP28 on the innate immunity of Litopenaeus vannamei. The activities of proPO, SOA and SOD significantly increased in haemocyte after dsRNA‐VP28 treatment, whereas MDA content did not change significantly. Among the ten immune‐related genes examined, only the mRNA expression of proPO, cMnSOD, haemocyanin, crustin, BGBP, lipopolysaccharides (LPs), lectin and lysozyme in haemocytes, gill and hepatopancreas of L. vannamei, was significantly upregulated at 12 h after dsRNA‐VP28 treatment, while no significant expression changes were observed in Toll receptor and tumour receptor genes. The increase of proPO and SOD activities, and SOA level and mRNA expression level of proPO, cMnSOD, haemocyanin, crustin, BGBP, LPs, lectin and lysozyme after dsRNA‐VP28 stimulation indicate that these immune‐related genes were involved in dsRNA‐VP28‐induced innate immunity in shrimp. 相似文献