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1.
《渔业科学进展》2015,(3)
研究了牛磺酸对半滑舌鳎(Cynoglossus semilaevis Günther)亲鱼繁殖性能、抗氧化功能、卵子及仔鱼质量的影响。在基础饲料中分别添加0、0.5%、1.5%的牛磺酸,配制3组(对照组T-0、T-0.5、T-1.5)等氮等脂的配合饲料,在室内流水养殖系统进行为期63 d的养殖实验。研究结果显示,在饲料中添加0.5%牛磺酸,不仅能够提高半滑舌鳎亲鱼的相对产卵量,还能促进亲鱼体内睾酮和雌二醇的分泌(P0.05);T-0.5组浮卵率、受精率、孵化率、卵径、油球直径、3日龄、7日龄仔鱼体长均显著高于对照组(P0.05),T-0.5组和T-1.5组仔鱼畸形率显著低于对照组(P0.05);3个实验组仔鱼生存活力指数(SAI)无显著性差异(P0.05);饲料中添加牛磺酸能够提高其肝脏和性腺中牛磺酸含量,降低肝脏中半胱胺双加氧酶活性;T-0.5组肝脏、血清、精巢、精液中超氧化物歧化酶(SOD)活性显著高于对照组(P0.05),性腺和卵子中丙二醛(MDA)含量显著低于对照组(P0.05)。研究结果表明,饲料中添加牛磺酸能够提高半滑舌鳎亲鱼繁殖性能、抗氧化功能、卵子及仔鱼质量。在本研究条件下,牛磺酸含量为0.5%的饲料组为最佳饲料组。 相似文献
2.
在饲料中分别添加0、20、100 IU/g的维生素A(视黄醇乙酸酯形式)配制成Ac、Al和Ah 3种试验饲料饲喂3龄半滑舌鳎亲鱼[雌性(1.58±0.18)kg/尾、雄性(0.20±0.05)kg/尾]70 d,探讨饲料中不同水平的VA对半滑舌鳎亲鱼繁殖性能的影响。结果显示,Al组和Ah组雌鱼性腺成熟率均要高于Ac组;相对产卵量Al组最高,Ac组最低;Al和Ah组的受精率和孵化率均显著高于Ac组(P0.05);Al组的卵上浮率、初孵仔鱼长度、仔鱼的成活率和仔鱼生存活力指数(SAI)均显著高于Ac组和Ah组(P0.05);随饲料中VA的增加仔鱼畸形率虽有增加趋势,但无显著性差异(P0.05);而卵巢、肝脏和卵子中VA的含量和血清中SOD的活性均随饲料中VA的增加而显著提高(P0.05)。此外,Al组和Ah组卵子中维生素C的含量以及卵子脂肪酸中n-3HUFA、n-6PUFA和PUFA的含量均要高于Ac组。综上所述,饲料中添加适量VA可促进半滑舌鳎亲鱼性腺的发育,提高产卵量,改进卵子和仔鱼的质量;本研究中Al组(20 IU/g)亲鱼的繁殖性能要优于Ac组(0 IU/g)和Ah组(100 IU/g)。 相似文献
3.
在饲料中分别添加0、200、1200 mg/kg的维生素E(DL-α-生育酚乙酸脂形式)配制成Ec、El和Eh三种试验饲料,饲喂3龄半滑舌鳎亲鱼(雌性1.58±0.19 kg/尾、雄性0.20±0.05 kg/尾)70 d,探讨饲料中添加不同水平的维生素E对半滑舌鳎亲鱼繁殖性能的影响。结果显示,饲料中添加1200 mg/kg的维生素E(Eh组)不仅可以促进亲鱼性腺的发育,提高亲鱼产卵量、浮卵率、受精率、孵化率以及初孵仔鱼长度,而且影响仔鱼质量,仔鱼的成活率、12 d仔鱼长度、生存活力等方面均要高于对照组。而饲料中添加200 mg/kg的维生素E(El组)只对亲鱼性腺的发育和初孵仔鱼长度有一定的促进作用,对亲鱼产卵量、浮卵率、受精率、孵化率没有显著影响(P>0.05)。随饲料中维生素E的增加,血清中SOD的活性显著升高(P<0.05),MDA含量则显著下降(P<0.05)。此外,El和Eh试验组卵中干物质、维生素C的含量,以及卵子脂肪酸中PUFA、n-3PUFA、n-3HUFA和n-6PUFA的含量均要高于Ec组,El和Eh试验组之间的没有显著性差异(P>0.05)。结果表明,在半滑舌鳎亲鱼饲料中添加适量维生素E会亲鱼促进性腺的发育,增加产卵量,提高卵子和仔鱼的质量。在本试验中Eh组(1200 mg/kg)亲鱼的繁殖性能要优于Ec组(0 mg/kg)和El组(200 mg/kg)。 相似文献
4.
《渔业科学进展》2015,(2)
在饲料中分别添加0、200、1200 mg/kg的维生素E(DL-α-生育酚乙酸脂形式)配制成Ec、El和Eh三种试验饲料,饲喂3龄半滑舌鳎亲鱼(雌性1.58±0.19 kg/尾、雄性0.20±0.05 kg/尾)70 d,探讨饲料中添加不同水平的维生素E对半滑舌鳎亲鱼繁殖性能的影响。结果显示,饲料中添加1200 mg/kg的维生素E(Eh组)不仅可以促进亲鱼性腺的发育,提高亲鱼产卵量、浮卵率、受精率、孵化率以及初孵仔鱼长度,而且影响仔鱼质量,仔鱼的成活率、12 d仔鱼长度、生存活力等方面均要高于对照组。而饲料中添加200 mg/kg的维生素E(El组)只对亲鱼性腺的发育和初孵仔鱼长度有一定的促进作用,对亲鱼产卵量、浮卵率、受精率、孵化率没有显著影响(P0.05)。随饲料中维生素E的增加,血清中SOD的活性显著升高(P0.05),MDA含量则显著下降(P0.05)。此外,El和Eh试验组卵中干物质、维生素C的含量,以及卵子脂肪酸中PUFA、n-3PUFA、n-3HUFA和n-6PUFA的含量均要高于Ec组,El和Eh试验组之间的没有显著性差异(P0.05)。结果表明,在半滑舌鳎亲鱼饲料中添加适量维生素E会亲鱼促进性腺的发育,增加产卵量,提高卵子和仔鱼的质量。在本试验中Eh组(1200 mg/kg)亲鱼的繁殖性能要优于Ec组(0 mg/kg)和El组(200 mg/kg)。 相似文献
5.
以平均体重为(8.13±0.05)g的鲈鱼(Lateolabrax japonicus)幼鱼为研究对象,探讨了在低鱼粉饲料中添加牛磺酸、蛋氨酸和半胱氨酸对鲈鱼幼鱼生长及鱼体氨基酸组成的影响.分别在基础饲料中添加0(对照组T-0)、1.0％牛磺酸(T-1)、2.0％牛磺酸(T-2)、0.5％蛋氨酸(M-0.5)和0.5％半胱氨酸(C-0.5)制成5种等氮等脂的实验饲料,在室内流水养殖系统中进行为期70 d的养殖实验.结果显示,T-1、T-2、M-0.5和C-0.5组鲈鱼幼鱼的终末体重、特定生长率(SGR)、增重率(WGR)和摄食率(FI)均显著高于T-0组(P＜0.05);饲料中添加牛磺酸、蛋氨酸和半胱氨酸均可提高鱼体粗蛋白和粗脂肪含量(P＜0.05),鱼体水分含量则呈现出相反的变化趋势;T-I、T-2组肝脏、肌肉中的牛磺酸含量显著高于T-0组(P＜0.05),但M-0.5、C-0.5组肝脏、肌肉中牛磺酸含量与T-0组无显著差异(P＞0.05);T-1、T-2和C-0.5组肝脏的必需氨基酸及总氨基酸含量均高于T-0组(P＜0.05),但M-0.5组肝脏必需氨基酸及总氨基酸含量与T-0组无显著差异(P＞0.05);T-1、T-2、M-0.5和C-0.5组肌肉的必需氨基酸含量均高于T-0组,但只有M-0.5组显著高于T-0组(P＜0.05);T-1、T-2和M-0.5组肌肉的总氨基酸含量高于T-0组(P＜0.05),C-0.5组与T-0组无显著差异(P＞0.05).研究表明,饲料中添加牛磺酸、蛋氨酸和半胱氨酸均可提高鲈鱼幼鱼的生长,同时可以改善鲈鱼肝脏和肌肉中的氨基酸沉积. 相似文献
6.
《渔业科学进展》2017,(4)
以平均体重为(8.13±0.05)g的鲈鱼(Lateolabrax japonicus)幼鱼为研究对象,探讨了在低鱼粉饲料中添加牛磺酸、蛋氨酸和半胱氨酸对鲈鱼幼鱼生长及鱼体氨基酸组成的影响。分别在基础饲料中添加0(对照组T-0)、1.0%牛磺酸(T-1)、2.0%牛磺酸(T-2)、0.5%蛋氨酸(M-0.5)和0.5%半胱氨酸(C-0.5)制成5种等氮等脂的实验饲料,在室内流水养殖系统中进行为期70 d的养殖实验。结果显示,T-1、T-2、M-0.5和C-0.5组鲈鱼幼鱼的终末体重、特定生长率(SGR)、增重率(WGR)和摄食率(FI)均显著高于T-0组(P0.05);饲料中添加牛磺酸、蛋氨酸和半胱氨酸均可提高鱼体粗蛋白和粗脂肪含量(P0.05),鱼体水分含量则呈现出相反的变化趋势;T-I、T-2组肝脏、肌肉中的牛磺酸含量显著高于T-0组(P0.05),但M-0.5、C-0.5组肝脏、肌肉中牛磺酸含量与T-0组无显著差异(P0.05);T-1、T-2和C-0.5组肝脏的必需氨基酸及总氨基酸含量均高于T-0组(P0.05),但M-0.5组肝脏必需氨基酸及总氨基酸含量与T-0组无显著差异(P0.05);T-1、T-2、M-0.5和C-0.5组肌肉的必需氨基酸含量均高于T-0组,但只有M-0.5组显著高于T-0组(P0.05);T-1、T-2和M-0.5组肌肉的总氨基酸含量高于T-0组(P0.05),C-0.5组与T-0组无显著差异(P0.05)。研究表明,饲料中添加牛磺酸、蛋氨酸和半胱氨酸均可提高鲈鱼幼鱼的生长,同时可以改善鲈鱼肝脏和肌肉中的氨基酸沉积。 相似文献
7.
以维生素A添加量为0(对照组)、10 000IU/kg(1万组)和80 000IU/kg(8万组)的3种人工配合饲料饲养大菱鲆Scophthamus maximus亲鱼8个月,通过比较各饲料组亲鱼平均相对产卵量、卵子上浮率、受精率、孵化率、卵子与仔稚鱼的大小等,研究不同添加水平的维生素A对亲鱼繁殖性能的影响。结果表明,8万组卵子上浮率和孵化率显著高于对照组(P<0.05),与1万组差异不显著(P>0.05);8万组初孵仔鱼体长2 684.1±24.91μm,显著高于对照组2 562.3±30.00μm(P<0.05),与1万组2 484.2±56.16μm差异不显著(P>0.05);卵子中n-3HUFA、n-6HUFA的含量和性腺中维生素A的含量随着饲料中维生素A添加量的增加而增加;8万组血清中SOD(超氧化物歧化酶,Superoxide dismutase)活力为91.88U/ml,显著高于对照组70.57U/ml(P<0.05)。 相似文献
8.
以精制鱼油、大豆油、橄榄油为脂肪源配制3组实验饲料,以一种商业饲料为对照组,进行连续56 d饲喂半滑舌鳎亲鱼试验,研究不同脂肪源对半滑舌鳎亲鱼繁殖性能、卵子脂肪酸组成和仔鱼质量的影响.结果显示,对照组亲鱼的相对产卵量最高,橄榄油组次之,鱼油组比橄榄油组低,豆油组最低.对照组的卵子畸形率最低为9.77％,鱼油组为14.53％,橄榄油组为19.4％,豆油组最高为35.17％(P＜0.05).同时对照组与鱼油组仔鱼畸形率分别为3.34％和3.12％,橄榄油组为8.85％,豆油组为17.35％ (P＜0.05).对照组的孵化率也最高,为95.49％,鱼油组次之,为84.88％,橄榄油组为79.57％,豆油组最低,为74.27％(P＜0.05).表明半滑舌鳎亲鱼的产卵期、卵子发育以及仔鱼的成长期都需要饲料中维持一定量n-3HUFA,特别是二十二碳六烯酸(DHA).油酸和亚油酸(LA)在半滑舌鳎卵子的发育过程被选择性保留下来,起着重要的作用.推断出用单一的植物油或者动物油作为脂肪源都不能很好地为其提供必需脂肪酸. 相似文献
9.
在基础饲料中分别添加0、800和4 800mg/kg的维生素C饲养大菱鲆亲鱼240d,通过比较各处理组亲鱼的生长、性腺指数、相对产卵量、上浮卵率、孵化率、卵径以及各组织中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量,研究维生素C对大菱鲆亲鱼繁殖性能的影响。实验结果表明,各处理组亲鱼生长无显著差异（P>0.05）,但随着饲料中维生素C添加量的增加,亲鱼的生长呈上升趋势。800和4 800mg/kg组亲鱼相对产卵量显著高于对照组（P<0.05）。随着饲料中维生素C添加量的增加,亲鱼性腺指数、产卵次数、上浮卵率、受精率和孵化率均显著提高（P<0.05）,亲鱼卵和组织中维生素C含量反映了饲料中的维生素C含量。虽然各处理组亲鱼的卵径和油球径差异均不显著（P>0.05）,但随着饲料中维生素C添加量的增加,亲鱼卵径和油球径呈上升的趋势。肝脏、卵巢和血清中SOD活性随饲料中维生素C添加量的增加而显著升高（P<0.05）,而MDA含量则显著下降（P<0.05）。综上所述,大菱鲆亲鱼饲料中添加维生素C,能有效改善大菱鲆的繁殖性能。 相似文献
10.
野生与人工养殖牙鲆亲鱼不同组织脂肪酸的比较 总被引：6，自引：1，他引：5
为研究脂肪酸对牙鲆繁育性能的影响, 采用生物化学方法, 对野生和养殖牙鲆亲鱼肌肉、肝脏及卵中脂肪酸组成分别进行测定。结果表明: (1) 牙鲆肌肉、肝脏和卵中脂肪含量大小关系为肝脏>卵>肌肉。野生亲鱼肌肉和卵中脂肪含量显著低于养殖亲鱼(P<0.05), 肝脏脂肪含量与养殖亲鱼无显著差异; (2) 牙鲆亲鱼3种组织中均检测出21种脂肪酸。野生亲鱼肌肉中饱和脂肪酸(SFA)与养殖亲鱼无显著差异, 单不饱和脂肪酸(MUFA)显著低于养殖亲鱼(P<0.05)。肝脏和卵中SFA显著高于养殖亲鱼(P<0.05), MUFA与养殖亲鱼无显著差异; (3) 野生亲鱼肌肉、肝脏和卵中高不饱和脂肪酸(PUFA)的含量, 尤其是肝脏和卵中C20:5n-3(EPA)、C22:6n-3(DHA)的含量均显著低于养殖亲鱼(P<0.05), 但肌肉和卵中的C20:4n-6(ARA)含量明显高于养殖亲鱼(P<0.05); (4) 野生牙鲆亲鱼肌肉、肝脏和卵中n-3/n-6 PUFA及EPA/ARA显著低于养殖亲鱼(P<0.05), 肝脏中DHA/EPA显著高于养殖亲鱼(P<0.05), 但野生亲鱼肌肉和卵中的DHA/EPA与养殖亲鱼无显著差异。比较结果说明, DHA、EPA和ARA等PUFA是与牙鲆繁殖性能密切相关的重要脂肪酸。在牙鲆亲鱼养殖过程中, 除了提供牙鲆亲鱼足够的脂肪酸营养外, 也应注意各种脂肪酸, 尤其是PUFA中各种脂肪酸之间的添加比例, 从而保证亲鱼的繁殖性能及卵和仔鱼的质量。 相似文献
11.
饲料中添加0、10％、20％的南极磷虾粉,配制3组(对照组K-0、K-10、K-20)等氮等脂的实验饲料,在室内流水养殖系统进行为期90 d的养殖实验,探讨南极磷虾粉对半滑舌鳎(Cynoglossus semilaevis)雄鱼繁殖性能和抗氧化能力的影响.结果显示,饲料中添加南极磷虾粉对半滑舌鳎雄鱼肝体比、性体比、精液浓度、睾酮含量均无显著性影响(P＞0.05);K-10组与K-20组精巢中超氧化物歧化酶(SOD)活性无显著性差异(P＞0.05),但均显著高于对照组(P＜0.05),K-10组与K-20组精巢、血清和肝脏中的丙二醛(MDA)含量无显著性差异(P＞0.05),但均显著低于对照组(P＜0.05).在本实验条件下,饲料中添加南极磷虾粉不能显著提高半滑舌鳎雄鱼的繁殖性能(P＞0.05),但能显著提高雄鱼的抗氧化功能(P＜0.05). 相似文献
12.
Antarctic krill (Euphausia superba) meal in the diets improved the reproductive performance of tongue sole (Cynoglossus semilaevis) broodstock 
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下载免费PDF全文 A three‐month feeding trial was conducted to investigate the effects of dietary krill meal on the reproductive performance of tongue sole broodstock. Three diets were formulated to contain different levels of krill meal, 0 (Diet K‐0), 10 (K‐100) and 200 (K‐200) g kg–1 dry matter, replacing the corresponding contents of protein and lipid from fishmeal and fish oil. Each diet was assigned to triplicate tanks. Compared with K‐0, both K‐100 and K‐200 increased the egg properties such as buoyant eggs rate, egg diameter, oil droplet diameter and SOD activity of the fertilized egg. However, the maturation rate, serum estradiol content, the relative fecundity and hatching rate were improved only by K‐100. The krill meal supplementation increased the concentrations of astaxanthin and n‐3 fatty acids in eggs, but decreased the n‐6 fatty acid concentrations. Both K‐100 and K‐200 increased the survival activity index, but the larval deformity rate was decreased only by K‐100. At 15 DPH, the larval length and the digestive enzyme activities were significantly higher in group K‐200 compared with groups K‐0 and K‐100. In conclusion, dietary supplementation of krill meal, at the level of 100 or 200 g kg–1 dry matter in exchange of fishmeal and fish oil had the considerable positive effects on C. semilaevis reproduction. 相似文献
13.
Hiroyuki Matsunari Kazuhisa Hamada Keiichi Mushiake Toshio Takeuchi 《Fisheries Science》2006,72(5):955-960
The effect of dietary taurine was investigated on reproductive performance in yellowtail Seriola quinqueradiata. Two-year-old fish of average body weight 6.1kg were fed on diets containing three levels of taurine (T-0, T-0.5 or T-1.0%)
for 5 months prior to spawning. For spawning investigations, fish were induced to maturity by human chorionic gonadotrop in
injection (600 IU/kg-fish) and artificially inseminated. Oocyte growth improved significantly (P<0.05) with the increase of
dietary taurine. The collection of eggs from females reared on the T-0% diet was not successful. The success rate of spawning
for females fed on T-0.5 and 1.0% diets was one out of six, and six out of seven, respectively. The taurine levels of the
liver and serum in the T-0% diet group were much lower than that in the T-0.5 and T-1.0% diet groups (P<0.05). Fish fed the
T-0% diet showed higher contents of serine in the liver and serum. The taurine content of the ovary was not significantly
different among the different dietary treatments. These results indicate that taurine has a positive effect on the improvement
of spawning performance of yellowtail. 相似文献
14.
Effect of graded levels of taurine on growth performance and Ptry expression in the tongue sole (Cynoglossus semilaevis) postlarvae 下载免费PDF全文
下载免费PDF全文 The effect of graded levels of dietary taurine on growth, enzyme activities and pretrypsinogen (Ptry) mRNA expression in the tongue sole postlarvae was evaluated in this study. Four microdiets supplemented with 0 (T0), 5 (T0.5), 10 (T1.0) or 20 (T2.0) g kg?1 taurine were prepared. These diets were fed to the postlarvae with an average initial body dry weight of 3.32 ± 0.06 mg for 28 days. Survival and growth performance of the tongue sole postlarvae were significantly (P < 0.05) influenced by dietary taurine. Survival rate of fish fed with 20 g kg?1 dietary taurine was significantly lower than other treatments. The growth of fish initially increased with increasing dietary taurine level and then decreased. Postlarvae fed with 10 g kg?1 dietary taurine showed the best growth performance. Trypsin activities showed a significant increase with increasing dietary taurine level and then reached a plateau, while amylase activities and alkaline phosphatase activities increased in the start with increasing dietary taurine and then decreased. Ptry mRNA expression was stimulated by dietary taurine. Excessive dietary taurine (20 g kg?1) had adverse effects on survival, growth, digestive enzyme activities of the experimental fish. The suggested dietary taurine supplementation in microbound diets was 10 g kg?1 for the tongue sole postlarvae. 相似文献
15.
The effect of vitamin A supplementation in broodstock feed on reproductive performance and larval quality in Penaeus chinensis 总被引：1，自引：0，他引：1
The effect of feeding four semi‐purified diets A1, A2, A3, A4, containing different vitamin A acetate levels 0, 20, 40, 60 mg kg?1 diet, respectively, on fecundity, egg hatching rate, larval survival rate and vitamin A content in eggs of Chinese shrimp (Penaeus chinensis) broodstock was compared with a fresh clam diet (control) in a 60‐day feeding trial. The broodstock shrimp fed the diet with 60 mg kg?1 vitamin A acetate added exhibited significantly higher fecundity (P < 0.01). Hatching rate was highest with diet A4 (P < 0.05), whereas hatching rates were similar fed diets A1, A2, A3. Increasing levels of vitamin A in broodstock diet resulted in improvement in larval quality. The vitamin A levels in shrimp eggs from broodstock fed with diet A4 were higher compared with those from broodstock fed with diet A1, A2 (P < 0.01). The fecundity and hatching percentages were positively correlated with the vitamin A content in eggs in the present study. The results of this study showed that higher level of vitamin A in broodstock diet may have positive effects on fecundity and larval quality in P. chinensis. 相似文献
16.
Mohammad Zakeri Jasem G. Marammazi Preeta Kochanian Ahmad Savari Vahid Yavari Mahsa Haghi 《Aquaculture (Amsterdam, Netherlands)》2009,295(1-2):99-105
A study was conducted to determine the effects of dietary protein and lipid concentrations on growth, spawning performance and egg quality of yellowfin sea bream, Acanthopagrus latus. Nine diets representing a combination of three protein concentrations (40, 50 and 60%) and three lipid concentrations (15, 20 and 25%) were tested with three replicates. Each replicate was stocked with 10 fish with a sex ratio of 1:1 and the average weights were 415 g and 236 g for females and males, respectively. Fish were fed to satiation twice daily. The weight gain of A. latus broodstock was not significantly (P > 0.05) different among fish fed diets with the various protein and lipid concentrations. Except for relative fecundity and survival rate of 3-day-post-hatch (3DPH) larvae, spawning performance of A. latus was not significantly affected by different concentrations of dietary protein or lipid. Relative fecundity was found to be significantly elevated by dietary lipid at 20% concentration. However, survival rate of 3DPH larvae was highest from broodstock fed diets with 40% protein and 25% lipid, 50% protein and 15% lipid and 60% protein and 15% lipid concentrations. Egg diameter generally increased with increasing dietary protein from 40 to 60% at each lipid concentration. Total length of hatchlings followed an inverse relationship with dietary protein regardless of the lipid concentration. The oil globule diameter (OGD) of hatchlings significantly increased with decreasing dietary protein from 60 to 40%. Yolk sac length (YSL) and OGD of hatchlings was generally greatest at 15% dietary lipid concentration irrespective of protein concentrations. Total length of 3DPH larvae was greatest from broodstock fed diets with 50% protein and 20% lipid, respectively. Body protein content of broodstock was not significantly affected by dietary protein and lipid concentrations. However, increasing dietary lipid generally resulted in increasing body lipid content regardless of the dietary protein concentration. Protein and lipid contents of eggs, hatchlings and 3DPH larvae were significantly affected by dietary protein and lipid concentrations of the broodstock diets. The present study revealed that the best reproductive performance of A. latus broodstock was achieved at 40% dietary protein and 20% dietary lipid concentrations based on relative fecundity, fertilization rate, hatchability and survival of 3DPH larvae. 相似文献
17.
Chatham K Callan Charles W Laidley Linda J Kling Nancy E Breen Andrew L Rhyne 《Aquaculture Research》2014,45(7):1176-1186
This study was conducted to determine the effects of dietary highly unsaturated fatty acids (HUFA) on flame angelfish (Centropyge loriculus) reproduction, and egg and larval quality. In the experiment, formulated diets containing 1.8, 2.9 or 3.6% n‐3 HUFA were fed to flame angelfish broodstock (n = 4) for 5 months. Mean fecundity (daily egg production), egg fertilization rates and embryo viability were used as indicators of egg quality. In addition, mean egg diameter, oil globule diameter, per cent hatch, larval size at hatch, per cent survival to yolk exhaustion and larval size at yolk exhaustion were recorded for each treatment. Flame angelfish fed the diet containing 3.6% n‐3 HUFA exhibited significantly increased fecundity, fertilization rates and embryo viability than fish that were fed the other two formulated diets. Egg diameter, egg oil globule diameter, larval size at hatch, larval survival to yolk exhaustion and larval size at yolk exhaustion from the 3.6% n‐3 HUFA broodstock treatment group were not significantly different from those derived from the Control broodstock treatment. These data revealed that flame angelfish egg quality could respond rapidly (within weeks) to maternal dietary changes. Results from this study further support that dietary HUFA composition can significantly affect broodstock reproductive performance as well as subsequent performance of eggs and larvae. 相似文献
18.
H. Furuita T. Yamamoto T. Shima N. Suzuki T. Takeuchi 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):725-735
This study investigated the effect of dietary arachidonic acid (AA) in broodstock of Japanese flounder on subsequent egg and larval quality. Diets with similar proximate composition and n-3 HUFA level, but with different AA levels (0.1%, 0.6% and 1.2% of diet), were fed to the broodstock from 3 months before and during the spawning season. Spawning was observed from March to May. Total egg production over the spawning season was highest in fish fed the 0.6% AA diet and lowest in fish fed the 1.2% AA diet. All parameters measured as egg quality (percentage of buoyant eggs, hatching rate, larval survival and normality of larvae.) were highest in fish fed the 0.6% AA diet. AA content in eggs proportionally increased with the dietary AA level. EPA content of polar lipids of eggs had a negative correlation with the AA level in diets whereas the DHA content was independent of dietary AA. The results of this study indicate that a supplement of AA at 0.6 g/100 g diet improved the reproductive performance of Japanese flounder, but a higher level of AA (1.2 g/100 g diet) negatively affected both egg and larval quality due to a potential inhibitory effect on EPA bioconversion. 相似文献