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1.
Motile aeromonad septicaemia caused by Aeromonas sobria is a cause of disease in farmed perch, Perca fluviatilis L., in Switzerland. We have evaluated the potential of a Pseudomonas chlororaphis isolate, obtained from perch intestine, to control A. sobria infection. Inoculation of juvenile perch with P. chlororaphis strain JF3835 prior to infection with A. sobria caused a reduction in A. sobria associated mortalities. Infection of perch with xylE -labelled P. chlororaphis indicated the bacterium is able to transiently colonize juvenile fish and fingerlings. 相似文献
2.
Pathogenic Aeromonas sobria has been identified as a causative agent of ulcerative disease in farmed European perch, Perca fluviatilis L. To study the effect of the normal intestinal bacterial flora of perch against A. sobria, we sampled 193 bacterial isolates from the perch digestive tract. The isolates were identified by sequence analysis of the 16S rRNA gene and their inhibitory potential against A. sobria was evaluated in vitro. Nineteen of the strains isolated showed inhibition and were also tested against other aeromonad and non-aeromonad fish pathogens including Yersinia ruckeri and Vibrio anguillarum. Isolates showing inhibition were primarily Pseudomonas spp.; however, inhibitory Shewanella spp., and Delftia sp. were also identified. A Pseudomonas chlororaphis isolate showed inhibition against all fish pathogens tested. 相似文献
3.
Dominant pathogenic species of mesophilic aeromonads isolated from diseased and healthy fish cultured in Poland 总被引：1，自引：0，他引：1
Kozińska A 《Journal of fish diseases》2007,30(5):293-301
Aeromonas isolates were collected from cultured fish, characterized phenotypically and identified to species using 16S rDNA. The pathogenicity of all isolates was assayed on the basis of haemolytic and proteolytic activity and challenge tests were performed for isolates from healthy fish. A total of 131 Aeromonas isolates were obtained and identified as follows: A. hydrophila (13), A. bestiarum (23), A. salmonicida (motile biogroup) (19), A. caviae (2), A. sobria (18), A. veronii bt. sobria (42), A. jandaei (1), A. encheleia (11) and A. allosaccharophila (2). All isolates of A. hydrophila and A. bestiarum and most isolates of A. salmonicida and A. veronii were classified as pathogenic. Aeromonas hydrophila was isolated only from diseased trout except for one isolate obtained from carp fry. The other potentially pathogenic Aeromonas species were present in diseased as well as healthy fish. The pathogenicity of isolates from healthy fish was correlated with their enzymatic activity and was also tested by challenge experiments. The dominant pathogenic species were A. veronii bt. sobria, A. bestiarum and A. salmonicida in common carp and A. hydrophila in rainbow trout. 相似文献
4.
2017年4月,四川省西昌某养殖场养殖的长丝裂腹鱼暴发一种以体表、内脏器官出血及角膜浑浊为特征的传染病。为探究此次疫病的病因,自病鱼的肝、脾和肾组织中分离到1株病原菌LXP170412,在脑心浸液琼脂上28℃培养48h,形成白色、半透明、表面光滑的圆形菌落,人工感染试验证实了其病原性;通过表型特征特性测定、16SrDNA和gyrB基因序列分析鉴定,分离菌LXP170412为温和气单胞菌。药敏试验结果表明,温和气单胞菌LXP170412对强力霉素、环丙沙星、氧氟沙星、诺氟沙星等敏感,对头孢拉定、新霉素、阿莫西林、庆大霉素、丁胺卡那、恩诺沙星、青霉素、罗红霉素等耐药。试验证实,温和气单胞菌为此次流行病的病原菌,药敏试验结果将为临床用药提供参考依据。 相似文献
5.
实验用鲫平均体重100g，采用免疫物质被动转移试验，探讨体液免疫在鲫感染温和气单胞菌（Aeromonas sobria)中的保护作用。结果表明，无论免疫物质是来自异源还是自源，是由相关的嗜水气单胞菌（A.hydrophia)诱导产生还是由攻毒的温和气单胞直接刺激，均具有良好的免疫保护作用。实验还发现，再次攻毒时，在所有抗体组的鲫存活率为100%时，全血被动转移组的死亡率高达55.6%；免疫前后免疫复合物发生明显变化。说明抗体在保护鲫免疫受温和气单胞菌的致死性感染中发挥决定性的作用。 相似文献
6.
研究从患病蝶尾金鱼(Butterfly)肝脏中分离获得的1株病菌,为金鱼疾病防治提供参考。分离纯化获得1株细菌,编号为DW-1,通过细菌形态观察、理化特性、16S rDNA序列分析等方法进行鉴定。结果表明DW-1菌株为革兰氏阴性短杆菌,可发酵葡萄糖产气,赖氨酸脱羧酶、硝酸盐还原等为阳性;进一步通过PCR方法扩增16S rDNA序列,测得长度为1 385 bp,与维氏气单胞菌(Aeromonas veronii)相似性为99%~100%;构建系统发育树与维氏气单胞菌温和生物变种(A.veronii biovar sobria)模式菌株ATCC9071聚为一支。最终鉴定该菌株为维氏气单胞菌温和生物变种。人工感染试验结果表明,对斑马鱼半数致死量(LD_(50))为1.17×10~6CFU/m L。药敏试验结果显示该菌株对头孢克肟、头孢哌酮、链霉素、恩诺沙星、诺氟沙星、左氟沙星、复方新诺明等23种药物敏感。 相似文献
7.
从患病甲鱼的肝脏中分离到9株细菌,其中7株为温和气单胞菌,2株为豚鼠气单胞菌。两种菌JA—1和JA—2对鲫鱼的半数致死量(LD_(50))各为4.3×10~5和5.5×10~7,在两种菌培养的上清液中均有一种具有溶血活性的毒素。研究表明:甲鱼的爆发性传染病是由温和气单胞菌和豚鼠气单胞菌引起,用两种菌为菌种做成菌苗,结合免疫增效剂免疫家兔制得血清,效价可达1:512,治愈率达100％。 相似文献
8.
黄金鲫温和气单胞菌鉴定及溶血素基因检测 总被引：1，自引：0，他引：1
采用常规的培养特征、理化特性及分子生物学的方法,从患病的黄金鲫体内分离到的优势菌YD-1进行了回感试验、表观生物学、药敏试验及毒力因子检测等系统性研究。结果表明,该菌在盐度1-3%、PH4-9,温度10-30℃的营养肉汤培养基中均能生长。该菌株16S rRNA基因序列（GenBank收录号: KC561935, 长度1446bp）和gyrB基因序列（GenBank收录号: KC561934, 长度1169bp）分别与其他气单胞菌属细菌的基因同源性相思似在97%-99%之间,构建系统发育树确定该菌株为温和气单胞菌(Aeromonas sobria)。人工回感试验可引起健康银鲫死亡。对该菌毒力因子进行检测,可扩增出溶血素基因片段。药敏试验显示：该菌对多粘菌素、头孢呋辛、米诺环素、复达新、菌必治、新霉素等6种药物敏感。 相似文献
9.
Identification and pathogenicity to rainbow trout, Oncorhynchus mykiss (Walbaum), of some aeromonads
Twelve strains of fish pathogenic aeromonads were identified by 16S rRNA sequencing as Aeromonas bestiarum , A. hydrophila , A. hydrophila subsp. dhakensis , A. salmonicida subsp. salmonicida , A. sobria biovar sobria and A. veronii biovar sobria. Following intramuscular injection, A. hydrophila subsp. dhakensis caused dark liquefying, raised furuncle-like lesions in rainbow trout within 48 h. Extracellular products of all cultures contained gelatinase and lecithinase, and most revealed lipase. Congo red absorption and siderophore production was recorded, but not so the suicide phenomenon or slime production. Sodium dodecyl sulphate polyacrylamide gel electrophoresis profile of the outer membrane proteins (OMP) revealed 10–25 bands, of which major bands were seen in the region of 32.5–47.5 and 62–83 kDa. Marked heterogenicity of the OMP and whole cell protein (WCP) profiles within and among the species was observed. Polypeptides of 83–173 kDa were detected in the WCP profile of the cultures, but they were not expressed in OMP fractions. 相似文献
10.
Melanie Rupp Paola Pilo Barbara Müller Ralph Knüsel Beat von Siebenthal Joachim Frey Paul‐Daniel Sindilariu Heike Schmidt‐Posthaus 《Journal of fish diseases》2019,42(5):685-691
In non‐salmonid fish, Aeromonas salmonicidacan cause local infections with severe skin ulcerations, known as atypical furunculosis. In this study, we present a systemic infection by a virulent A. salmonicidain European perch (Perca fluviatilis).This infection was diagnosed in a Swiss warm water recirculation aquaculture system. The isolate of A. salmonicida encodes a type three secretion system (TTSS) most likely located on a plasmid similar to pAsa5/pASvirA, which is known to specify one of the main virulence attributes of the species A. salmonicida. However, the genes specifying the TTSS of the perch isolate show a higher temperature tolerance than strains isolated from cold‐water fish. The function of the TTSS in virulence was verified in a cytotoxicity test using bluegill fry and epithelioma papulosum cyprinid cells. 相似文献
11.
根据NCBI公布的温和气单胞菌(Aeromonas sobria)丝氨酸蛋白酶的基因序列,设计并选取一对能够快速而准确地检测温和气单胞菌的PCR引物,建立PCR快速检测体系,并对患病的鱼组织进行检测。研究结果表明,使用设计的引物能够扩增出与预计大小相符合的131 bp的特异性片段,具有较好的检测特异性,对靶标DNA的检测灵敏度为1.0 pg/20μL,对温和气单胞菌的检测灵敏度为50 cfu/20μL。样品的检测结果与实际发病情况一致,表明本研究成功建立了温和气单胞菌常规PCR检测体系,该体系可以用于温和气单胞菌的诊断和样品的检测。 相似文献
12.
丁鱥溃疡病的病原菌分离、鉴定与人工感染试验 总被引：3，自引：0，他引：3
采用细菌常规分离方法分离丁鱥溃疡病病原，并对所获得的纯培养物进行VITEK-32鉴定和人工感染试验，结果显示：维罗纳气单胞菌温和其变种是丁鱥溃疡病的主要病原菌。 相似文献
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15.
斑点叉尾暴发性败血症病原的分离与鉴定 总被引：4，自引：2，他引：4
从3尾表现出血症状的患病斑点叉尾鱼回(Ictalurus Punctatus)体内各分离出一株病菌,测定结果显示致其急性死亡的致病菌为温和气单胞菌(Aerom onas sobria),慢性死亡的致病菌分别为嗜水气单胞菌(A.hydrophila)和温和气单胞菌(A.sobria)。药敏试验结果表明:3株菌对广谱抗生素如氨苄青霉素、青霉素、先锋霉素Ⅵ和红霉素等已产生耐药性,而对菌必治、头孢肤肟和复达欣等高度敏感;菌株Jh-1和菌株Yh-3对复方新诺明、四环素、氟哌酸和丁胺卡那霉素高度敏感,而菌株Jh-2却低度敏感;环丙沙星和诺氟沙星对3株致病菌均具较好的抑菌、杀菌作用。 相似文献
16.
金鱼致病菌的分离鉴定与药敏特性 总被引：1，自引：0，他引：1
采用常规方法对患病金鱼(Carassius auratus)内脏组织(肝脏、肾脏、性腺)中致病菌进行了分离鉴定与药敏特性研究。结果显示:实验共分离了8株优势菌,经人工回归感染实验证实其中3株细菌(菌株LK-T、PL-R和PK-T)具有致病性。ATB细菌鉴定仪鉴定结果显示,菌株LK-T和菌株PL-R为温和气单胞菌(Aeromonas so-bria),菌株PK-T为豚鼠气单胞菌(Aeromonas punctata subsp.caviae)。这3株致病菌的主要特征为:革兰氏染色阴性,杆状,对奈替米星、头孢呋肟、头孢拉啶、庆大霉素、环丙沙星、新霉素、先锋必等药物均高度敏感。其中庆大霉素、环丙沙星、新霉素等常规渔药可作为防治用药的依据。 相似文献
17.
鳖保宁对中华鳖气单胞菌病的药效学研究 总被引：7，自引：2，他引：7
采用常规方法对中华鳖（Trionyx sinensis)常见病害进行细菌学检查，测定了鳖保宁及相关单味药对中华鳖主要病原菌温和气单胞菌（Aeromonas sobria)和豚鼠气单胞菌（A.caviae)的最低抑菌浓度（MIC）和最低杀菌浓度（MBC），并观察鳖保宁预防鳖病害的效果，结果表明：20例患病鳖中共分离到28株细菌，其中温和气单胞菌和豚鼠气单胞菌分别占32.14%和21.43%;鳖保宁及相关单味药对温和气单胞菌和豚鼠气单胞菌均有不同程度的抑制和杀灭作用。其中鳖保宁的作用最强；鳖保宁预防鳖病害的效果优于对照组。 相似文献
18.
Identification of pathogenic bacteria associated with the occurrence of 'red spot' in sea mullet, Mugil cephalus L., in south-eastern Queensland 总被引：2，自引：0，他引：2
Abstract. An investigation was undertaken to determine the bacterial species associated with the fish disease known as 'red spot' which affected wild fish stocks in south-eastern Queensland. Outbreaks of the disease in the Noosa River were examined with particular reference to the sea mullet, Mugil cephalus. The bacterial flora of the skin of healthy fish as well as those exhibiting early and advanced lesions was determined. In the lower Noosa River estuary, Vibrio anguillarum was the sole organism associated with very early lesions. It was later shown that V. alginolyticus was also associated with V. anguillarum in early lesions, but was considered to be a secondary invader. Aeromonas hydrophila was isolated from advanced lesions on fish taken in freshwater reaches of the river. This organism was also regarded as a secondary invader. 相似文献
19.
采用尼罗罗非鱼上皮细胞培养模型,观察嗜水气单胞菌对鳃上皮、皮肤上皮、肠上皮细胞的粘附率,研究载铜蒙脱石对细菌粘附的阻断作用及其对细菌粘附引起鱼上皮细胞膜生物特性变化的影响。结果显示：嗜水气单胞菌与鱼上皮细胞均有不同程度的粘附作用,粘附率大小顺序为鳃上皮〉皮肤上皮〉肠上皮细胞。载铜蒙脱石均显著降低了嗜水气单胞菌对鳃、皮肤和肠上皮细胞的粘附率（P〈0．05）,而且对不同上皮细胞的粘附阻断率无显著差异。嗜水气单胞菌粘附肠上皮细胞后细胞膜生物特性发生了显著变化,与正常对照组相比,嗜水气单胞菌粘附鱼上皮细胞后,胞浆游离钙和细胞膜磷脂酶A2活性显著上升。载铜蒙脱石可显著降低由于嗜水气单胞菌粘附鱼上皮细胞引起的胞浆游离钙和细胞膜磷脂酶A2活性的升高。结果表明,载铜蒙脱石可有效阻断病原菌粘附,从而防治细菌感染和细菌移位。 相似文献
20.
Antagonistic activity of Aeromonas media strain A199 against Saprolegnia sp., an opportunistic pathogen of the eel,Anguilla australis Richardson 总被引：3，自引：0，他引：3
A bacteriocin-like inhibitory substance (BLIS) produced by Aeromonas media strain A199 inhibited the growth of Saprolegnia sp. in vitro, an opportunistic pathogen isolated from affected eels, Anguilla australis (Richardson). The presence of BLIS in solid media inhibited the growth of the vegetative state of the aquatic mould as well as the germination of cysts. Uninhibited growth was, however, observed in the presence of inactive BLIS, suggesting that the in vitro antagonism derived from the BLIS of A199. In four independent in vivo tank observations of fish affected with saprolegniosis, the daily addition of A199 to tank water contributed to the subsequent swift recovery of affected hosts from invasion by this opportunistic pathogen. 相似文献