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1.
The endemic, anadromous cyprinidChalcalburnus tarichi is the only fish species known to occur in alkaline Lake Van (Eastern Anatolia, Turkey). EightC. tarichi were maintained individually in Lake Van water (17 – 19°C; pH 9.8; 153 mEq·I–1 total alkalinity; 22 total salinity) and tank water samples analyzed for 24 h in 2 to 4 h intervals. At zero time, < 1µM ammonia was present and urea was undetectable in the tank water; at 24 h, total ammonia and urea made up 114±32 and 35±25µM, respectively. Over the experimental period, ammonia-N and urea-N excretion averaged 1041±494 and 607±169moles·kg–1 fish·h–1, respectively. The extent of urea excretion was highly variable between specimens. Uric acid excretion was not detectable.Urea was present at high concentrations in all tissues and plasma (25 – 35moles·g–1·ml–1) of freshly caughtC. tarichi; total ammonia content of the tissues was by a factor of 1.9 (liver) to 3.0 (brain) lower. High arginase activity (2.4±0.2 U·min–1·g–1) was detected in the liver ofC. tarichi but ornithine carbamoylphosphate transferase, a key enzyme of the ornithine-urea-cycle, was absent. Ureagenesis is likely through degradation of arginine and/or uricolysis. High glutamine synthetase activity (11±0.6 U·min–1·g–1) and low ammonia content in brain suggest that, like other teleosts,C. tarichi has an efficient ammonia detoxification in the brain, but in no other tissue.Nitrogenous waste excretion at alkaline pH is discussed. The ability ofC. tarichi to excrete high levels of ammonia at extremely alkaline pH is unique among teleosts studied so far. The mechanism of ammonia excretion under Lake Van conditions remains to be elucidated.  相似文献   

2.
This study presents a simple urease method for analysis of ammonia and urea in freshwater aquaculture systems. Urea is hydrolysed into ammonia using urease followed by analysis of released ammonia using the salicylate‐hypochlorite method. The hydrolysis of urea is performed at room temperature and without addition of a buffer. A number of tests were performed on water samples obtained from a commercial rainbow trout farm to determine the optimal urease concentration and time for complete hydrolysis. One mL of water sample was spiked with 1.3 mL urea at three different concentrations: 50 μg L?1, 100 μg L?1 and 200 μg L?1 urea‐N. In addition, five concentrations of urease were tested, ranging from 0.1 U mL?1 to 4 U mL?1. Samples were hydrolysed for various time periods ranging from 5 to 120 min. A urease concentration of 0.4 UmL?1 and a hydrolysis period of 120 min gave the best results, with 99.6–101% recovery of urea‐N in samples spiked with 100 or 200 μg L?1 urea‐N. The level of accurate quantification of ammonia using the method is 50 μg L?1 NH4+‐N, and the detection level is 5–10 μg L?1 NH4+‐N.  相似文献   

3.
Ammonia distribution and excretion in fish   总被引:6,自引:0,他引:6  
This paper reviews the literature concerning ammonia production, storage and excretion in fish. Ammonia is the end product of protein catabolism and is stored in the body of fish in high concentrations relative to basal excretion rates. Ammonia, if allowed to accumulate, is toxic and is converted to less toxic compounds or excreted. Like other weak acids and bases, ammonia is distributed between tissue compartments in relation to transmembrane pH gradients. NH3 is generally equilibrated between compartments but NH4 + is distributed according to pH. Ammonia is eliminated from the blood upon passage through the gills. The mechanisms of branchial ammonia excretion vary between different species of fish and different environments, and primarily involves NH3 passive diffusion and NH4 +/Na+ exchange. Water chemistry near the gill surface may also be important to ammonia excretion, but a more accurate measurement of the NH3 gradient across the gill epithelium is required before a more detailed analysis of NH3 and NH4 + excretion can be made.  相似文献   

4.
Empirical data on ammonia excretion rates were compiled from several published and unpublished growth studies on post-smolt Atlantic salmon, Salmo salar L. Fish in all studies were fed to satiation with commercially produced high-energy diets (ME = 18–19 MJ kg-1) with a protein content of 40–45%. About 35 ± 3% (mean SE) of the nitrogen supplied to fish was excreted as total ammonia (TAN = NH3-N + NH4+-N). The results of a linear regression analysis of N intake to N excretion demonstrated, however, that TAN excretion rates could be divided into two components: TANexcretion [g N kg fish-1 day-1] = 0.036 + 0.26 Nintake [g N kg fish-1 day-1]. The intercept of the regression equation indicates that the endogenous TAN excretion rates in post-smolts could be estimated as 36 mg TAN kg fish-1 day-1, and about 26% of the nitrogen supplied to the fish was excreted postprandially. This postprandial TAN excretion was lower than that from other salmonid species fed low-energy diets. The daily maximum TAN excretion rate was about 43% higher than daily mean values, which agree with several studies. The ammonia quotient (A.Q.) measured was independent of the nitrogen supplied, and was calculated as 0.112. The outputs from the present model were compared to those from other ammonia excretion models.  相似文献   

5.
The theory of non-ionic diffusion predicts that ammonia will distribute between intracellular and extracellular tissue compartments according to transmembrane pH gradients. The distribution of ammonia and14C-DMO were compared in white muscle and plasma of rainbow trout (Salmo gairdneri) at rest, and following exhaustive exercise. Under both experimental conditions, intracellular ammonia levels far exceeded those predicted by transmembrane pH gradients. Calculated equilibrium potentials for ) were very close to published resting values of membrane potential Em in fish white muscle. We conclude that NH 4 + is permeable across cell membranes and that intracellular ammonia stores are not determined by pH gradients.The term ammonia or Tamm will be used to indicate the total ammonia concentration, while NH 4 + and NH3 will refer to ammonium ion and nonionic ammonia, respectively.  相似文献   

6.
This paper presents the first data on ammonia excretion by fed and starved juvenile common tench. The aim of the study was to determine the amount of ammonia excretion by juvenile common tench Tinca tinca (L.), fed with a commercial feed under intensive rearing conditions. Rearing was conducted for a period of 4 weeks at a water temperature of 27 °C. On the test day, the amounts of ammonia excreted by starved fish (over 12 h: control group) and fish fed with a morning feed dose (3 % of biomass: experimental group) were determined at 1, 3, 5, 7, 9 and 11 h after feeding. Ammonia excretion measurements were taken in 7-day intervals. Despite the increased growth rate in successive weeks of experiment, the amount of ammonia excreted by the fish was always at a similar level within the control (maximum 0.26 mg g?1) and the experimental group (maximum 0.39 mg g?1). This paper presents for the first time reliable amount of ammonia excreted by fed and starved juvenile common tench. The obtained results may also increase the effectiveness of intensive rearing procedures (taking into account stocking density and feeding regime) and allow to design the most effective biofiltration capacity of recirculating aquaculture systems for the commercial production of this species.  相似文献   

7.
The pattern of changes of activity of the urea cycle enzymes and the rate of urea-N excretion were studied in the perfused liver of an Indian air-breathing ureogenic walking catfish, Clarias batrachus. The liver was perfused with different concentrations of NH4Cl for a period of 60 min to determine the role of ammonia for stimulation of hepatic ureogenesis and the threshold level of ammonia loading needed to cause such stimulation. Both the urea-N excretion and the ammonia uptake by the perfused liver were found to be a saturable process. Ammonia accumulated significantly in the liver infused with 1.25 moles g liver –1 min–1 of NH4Cl, followed by a maximum accumulation of about 28.5 moles g wet wt–1 with the infusion of 5.08 moles g liver–1 min–1. The Vmax of the urea-N excretion (0.47 mol g liver–1 min–1) was obtained with the addition of 5.08 moles g liver–1 min–1 of NH4Cl. Both the tissue and the specific activity of the urea cycle enzymes, except ornithine transcarbamylase and arginase, were stimulated significantly with the infusion of either 1.25 or 5.08 moles g liver–1 min–1 of NH4Cl. Maximum stimulation of tissue activity of carbamoyl phosphate synthetase (about 120%) was seen with the infusion of 5.08 mol g liver–1 min–1, and for argininosuccinate synthetase (about 135%), and argininosuccinate lyase (about 50%) with the infusion of 10.81 mol g liver–1 min–1 of NH4Cl. Higher accumulation of ammonia of about 10–15 mol g wet wt–1 from the physiological level in the perfused liver while infusing with NH4Cl was suggested to be one of the major causes of stimulation of ureogenesis. The presence of such physiological adaptive strategy is probably necessary in this unique group of air-breathing walking catfish to survive under hyper-ammonia stress in their normal habitat or while living outside water or while burrowing inside mud.  相似文献   

8.
Yolk-sac larvae and starved larvae of Clarias gariepinus (Burchell 1822), reared at 28°C, were predominantly ammonotelic, but urea excretion contributed about 19±7% to the total nitrogen excretion. Exogenously feeding larvae of C. gariepinus were mainly ammonotelic until 180-205 h post fertilization, but from then on the relative urea excretion stabilized at 44±13%. The contents of total free amino acids (FAA) and ammonia in C. gariepinus peaked around complete yolk absorption. During the first period after hatching 64% of the FAA and 60% of the total ammonia were located in the yolk compartment. The body compartment contained the highest amount of FAA and total ammonia at the end of the yolk-sac period (65 and 77%, respectively). The amount of nitrogen originating from catabolism of amino acids was balanced within 6% by the excreted sum of ammonia and urea. The high degree of ureotelism in C. gariepinus larvae may constitute an adaptive mechanism to a habitat of temporal water shortage.  相似文献   

9.
We examined the ionoregulatory responses to temperature changes in two species of freshwater fish that differ in thermal preferences; the stenothermal, cold-water rainbow trout (Oncorhynchus mykiss) and the more eurythermal, warm-water common shiner (Notropis cornutus). We found that rainbow trout maintained constant plasma Na+ levels over the entire temperature regime (5–20 °C). Upon transfer from 15 °C (holding temperature) to 5 and 10 °C, rainbow trout experienced a significant drop in Na+ uptake (Jin Na), but after two weeks Jin Na had upregulated to warm temperature levels. Further, Na+ efflux (Jout Na) fell significantly at the colder temperatures. As a result, trout at the lowest temperatures were still in ion balance. When trout were exercised to exhaustion both O2 consumption (MO2) and Jout Na rose significantly at all temperatures, but while MO2 continued to be dependent upon temperature, Jout Na was high and constant. In contrast to the trout, common shiners experienced a 20% drop in plasma Na+ at 5 °C. Upon exposure to cold temperatures they experienced a reduced Jin Na, and showed no signs of acclimation during the subsequent two weeks. Likewise Jout Na was constant at all temperatures. These findings raise questions regarding the degree to which fish employ homeostatic mechanisms designed to defend a set- point (i.e., steady-state) osmolarity and ionic composition.  相似文献   

10.
The oxygen consumption (02) and ammonia excretion (N) of juvenile and adult common wolffish was measured in culture tanks in the laboratory. The oxygen consumption and ammonia excretion were affected by temperature (C), fish size and feeding rate. For juveniles (0.5 kg; 7C) the diel oxygen consumption rate varied between 37 and 62 mg O2 kg-1 h-1 and ammonia excretion between 2.3 and 5.7 mg N kg-1 h-1. The corresponding rates for adult fish (6.9 kg; 7C) were 29-44 mg O2 kg-1 h-1 and 1.2–3.1 mg N kg-1 h-1. The weight-specific oxygen consumption (mg O2 kg-1 h-1) was described by the following formulae: O2 (7C) = 0.17* W0.83 and O2 (12C) = 0.39 * W0.73 and the corresponding ammonia excretion (mg N kg-1 h-1) by: N(7C) = 0.024 W0.75 and N(12C) = 0.073 W0.60, where W is fish weight in g.  相似文献   

11.
Oxygen consumption, carbon dioxide excretion and nitrogen excretion from 2 kg Atlantic salmon Salmo salar L. were studied at land-based outdoor tanks, throughout a 10 day period. Fish were fed six feed rations (0 [fasting fish], 0.15, 0.30, 0.45, 0.60 and 0.75% BW day-1) of commercially extruded dry feed at two provision regimes: between 07.00-09.00 h and 19.00–21.00 h (periodic feeding); and between 07.00-21.00 h (continuous feeding). Fish were acclimatized to the feeding regime for 5 days prior to the start of the experiment. From days 5 to 10 of the study, oxygen consumption was measured automatically every 15 min. During day 10, carbon dioxide excretion, ammonia and urea excretion were measured hourly throughout a 24 h period. During the experiment, the water temperature and salinity were 8.5C and 33.5 ppt, respectively. Significant linear relationships between feed ration and metabolic rates were evident. Increased feed ration influenced oxygen consumption, carbon dioxide excretion, ammonia and urea excretion, ammonia quotient and the respiration quotient, proportionally. The two feed provision regimes caused the establishment of different daily rhythms in metabolic excretion. Only small differences in total daily excretion were however recorded for each feed ration. Ammonia and respiratory quotient results clearly showed that fish became more dependent on fat oxidation as an energy source, when feed was restricted.  相似文献   

12.
The growth efficiency of juvenile Atlantic halibut, Hippoglossus hippoglossus (L.), was investigated by comparing wet weight specific growth rates (SGRs) over 28 days with protein synthesis rates measured using a non-invasive stable isotope (15N) tracer technique. A diet containing 15N-labelled protein was fed at a single meal and individual feed intake was measured by X-ray radiography to allow calculation of the amount of N consumed. Excreted ammonia and urea were collected from each fish and the ratio of 15N to 14N was used to calculate rates of protein synthesis. Peak rates of ammonia excretion occurred 12 h post-feeding. Rates of urea excretion did not change after feeding and remained relatively constant over the 48-h measurement period. Urea accounted for 17% of the measured nitrogen excretion and showed no enrichment with N. Mean protein growth rates were 1.31 ± 0.06% day?1, while whole animal fractional rates of protein synthesis were 2.02 ± 0.24% day?1. The mean protein synthesis retention efficiency was 77.41 ± 9.09%, which is higher than that recorded for most other teleosts. This suggests that halibut have a relatively low cost, high growth efficiency growth strategy.  相似文献   

13.
This study examined ammonia, urea, creatinine, protein, nitrite, nitrate, and phosphorus (P) excretion at different water hardness, humic acid, or pH levels in silver catfish (Rhamdia quelen) juveniles. The fish were exposed to different levels of water hardness (4, 24, 50, or 100 mg L?1 CaCO3), humic acid (0, 2.5, or 5.0 mg L?1), or pH (5.0, 6.0, 7.0, 8.0, or 9.0) for 10 days. The overall measured nitrogen excretions were 88.1 % (244–423 μmol kg?1 h?1) for ammonia, 10.9 % (30–52 μmol kg?1 h?1) for creatinine, 0.02 % (0.05–0.08 μmol kg?1 h?1) for protein, 0.001 % (0.002–0.004 μmol kg?1 h?1) for urea, 0.5 % (0.64–3.6 μmol kg?1 h?1) for nitrite, and 0.5 % (0.0–6.9 μmol kg?1 h?1) for nitrate, and these proportions were not affected by water hardness or humic acid levels. The overall P excretion in R. quelen was 0.14–2.97 μmol kg?1 h?1. Ammonia excretion in R. quelen usually was significantly higher in the first 12 h after feeding, and no clear effect of water hardness, humic acid levels, and pH on this daily pattern of ammonia excretion could be observed. Water hardness only affected the ammonia and P excretion of R. quelen juveniles in the initial and fifth days after transfer, respectively. The exposure of this species to humic acid increased ammonia excretion after 10 days of exposure but did not affect P excretion. An increase in pH decreased ammonia and increased creatinine excretion but did not change P excretion in R. quelen. Therefore, when there is any change on humic acid levels or pH in the culture of this species, nitrogenous compounds must be monitored because their excretion rates are variable. On the other hand, P excretion rates determined in the present study are applicable to a wide range of fish culture conditions.  相似文献   

14.
高碱环境下青海湖裸鲤氮废物排泄及相关基因的表达规律   总被引:1,自引:1,他引:0  
采用个体生理学及定量PCR方法,研究了青海湖裸鲤(Gymnocypris przewalskii)在32 mmol/L(CA32)和64 mmol/L(CA64)碳酸盐碱度胁迫下氮废物排泄规律及鳃和肾组织中Rhesus type b glycoproteins(Rhbg)、Rhesus type c2 glycoproteins(Rhcg2)与urea transporter(Ut)基因的表达规律。结果显示,在32 mmol/L碱度胁迫整个过程中及64 mmol/L碱度胁迫初期裸鲤氨氮排泄率显著降低,但在64 mmol/L碱度胁迫8~20 h、24~72 h时氨氮排泄率基本恢复到胁迫前水平,在32 mmol/L碱度胁迫12~16 h、20~24 h及64 mmol/L碱度胁迫16~48 h时尿素氮排泄率显著升高。定量PCR结果显示,Rhbg、Rhcg2、Ut基因在胁迫过程中都有表达上调趋势,其中32 mmol/L碱性环境下鳃组织中Rhbg基因在胁迫12 h时表达明显上调;64 mmol/L碱性环境下鳃组织中Rhcg2基因在胁迫6 h、48 h、72 h表达明显上调,Ut基因在胁迫6 h表达明显上调,肾组织中Rhcg2基因在胁迫6 h表达明显上调。以上结果表明,裸鲤在高碱环境下虽然前期氮废物排泄受到抑制,但后期会通过启动Rh基因高表达恢复氨氮排泄,同时启动Ut高表达来增加尿素氮排泄来进行氮废物排泄。这一特殊氮废物排泄策略有助于裸鲤更好地适应高碱性环境。  相似文献   

15.
Nitrogen excreted by aquatic animals mainly takes the form of metabolic wastes such as feces and ammonia, which is accumulated in the intensive aquaculture system and causes serious environmental contamination. So it is very important to determine the waste excretion characteristics of aquatic animals for the development of practical and nonpolluting land‐based aquaculture. Abalone has a unique feeding habit and feeding regime, different from those of finfish; abalone gnaw feed seaweed to produce feces and ammonia continuously. In this study, the rates of feces production and ammonia excretion of pacific abalone, Haliotis discus hannai, of three shell lengths (3, 5, and 7 cm) were investigated under three different temperature conditions (12, 16, and 20 C). All experiments were performed in triplicate in a semirecirculating aquaculture system. Feces were collected for 5 d, and ammonia concentrations (total ammonia nitrogen [TAN]) in the tank inlet and outlet were monitored every 4‐h interval for 24 h at the fourth day of the feces collection. The regressions for the weight‐specific feces production rate (g feces/kg abalone/d) and the weight‐specific TAN excretion rate (mg TAN/kg abalone/d) in relation to water temperature (T, C) and shell length (L, cm) were weight‐specific feces production rate = exp(1.575 ? 281.2/T2 – 0.142L), r2 = 0.9550, and weight‐specific TAN excretion rate = exp(5.052 ? 277.1/T2 ? 0.136L), r2 = 0.9598. Pacific abalone produced 108.3–111.7 g feces and excreted 3.83 g TAN/kg seaweed ingested (dry weight).  相似文献   

16.

Gilthead sea bream (Sparus aurata) was raised in six individual recirculating aquaculture systems (RAS) whose biofilters’ performance was analyzed. Fish were fed with three different diets (a control diet, a fishmeal-based diet (FM), and a plant meal-based diet (VM)) and with three different feeding strategies (manual feeding to apparent satiation, automatic feeding with restricted ration, and auto-demand feeding). For every combination of diet and feeding strategy, the mean oxygen consumption, ammonia excretion, and ammonia removal rate were determined. Fish fed with the VM diet consumed the most oxygen (20.06?±?1.80 gO2 consumed kg?1 day?1). There were significant differences in ammonia excretion depending on the protein content and protein efficiency of the diet, as well as depending on feeding strategy, which in turn affected ammonia removal rates. Fish fed by auto-demand feeders led to the highest mean ammonia removal rate (0.10 gN-TAN removed m?2 biofiltration area day?1), while not leading to peaks of high ammonia concentration in water, which preserve fish welfare and growth.

  相似文献   

17.
This study determined the digestibility of nitrogen and phosphorus, and the excretion rate of different‐sized groups of milkfish fed a commercial diet, a SEAFDEC formulated diet or lab‐lab (natural food‐based diet). Fish (31.2–263.0 g) were stocked in 12 units of 300‐L fibreglass tanks filled with aerated seawater. The postprandial total ammonia‐nitrogen (TAN) and phosphate (PO4‐P) excretion of fish were estimated from changes in TAN and PO4‐P concentrations in water for 24 h. Digestibility was determined from the nitrogen, phosphorus and Cr2O3 content of the diets, and pooled faeces after the fish had been fed diets marked with chromic oxide. TAN excretion rate (mg TAN kg?1 fish day?1) was significantly lowest (P < 0.05) in medium to very big fish fed the lab‐lab diet (60.8–124.4) and highest in small and medium fish fed the SEAFDEC diet (333.3–331.6) and small fish fed the commercial diet (280.1). Regardless of size, fish fed lab‐lab excreted (mg PO4‐P kg?1 fish day?1) significantly lower PO4‐P (36.2) but did not differ with fish fed the commercial diet (64.8). Excretion rates decreased exponentially as fish weight increased but positively increased with feed ration. Excretion pattern of milkfish revealed two peaks: the first peak occurred 6 h after feeding and the second peak at 18 h for TAN and 21 h for PO4‐P, coinciding with the start of the daylight hours. TAN and PO4‐P excretion accounted for 20.5–34.6% of total N consumed and 18.7–42.6% of P consumed respectively. Approximately 27.9–42.5% of N consumed and 47.2–58.5% of P consumed were lost as faeces. Total nutrient losses were lower using the lab‐lab diet (0.31 g N and 0.14 g P kg?1 fish) compared with the formulated diets (0.47–0.48 g N and 0.17–0.19 g P kg?1 fish); the losses decreased per kg of fish as fish size increased. Results suggest that the diet and size of fish influence wastage of N and P to the environment with greater losses in small fish and when artificial diets are used. Such measurements will provide valuable information for the preparation of N and P budgets for milkfish in grow‐out systems.  相似文献   

18.
In light of recent evidence that carrier-mediated transport of urea occurs in the mammalian kidney, this study examined the renal handling of urea in freshwater rainbow trout (Oncorhynchus mykiss). Fish were fitted with indwelling arterial and urinary bladder catheters for the measurement of plasma and urine composition (urea, Na+, Cl-, glucose, H2O), glomerular filtration rate ([3H]PEG-4000), and urine flow rate, thereby allowing quantification of tubular reabsorption rates. The fractional reabsorption of urea (72%) was greater than that of H2O (50%) but less than that of Na+, Cl-, or glucose (95–100%) and occurred against an apparent concentration gradient, suggesting active reabsorptive transport; [urea] in the urine was only 59% of that in blood plasma. When fish were infused with exogenous urea loads, these patterns remained largely unchanged, and urea reabsorption increased in direct proportion to the filtered urea load. There was no evidence for saturation of the reabsorptive transport mechanism at urea filtration rates up to 4-fold above the normal range, representing urea loading rates that proved toxic. Extra-renal excretion, presumably through the gills, increased markedly, almost keeping pace with urea loading. This evidence suggests that carrier-mediated reabsorptive transport of urea occurs in the kidney and that plasma urea levels are normally subject to tight homeostatic control in freshwater trout.  相似文献   

19.
During gradual air exposure, Amia calva show no reduction in oxygen consumption, no increase in plasma urea levels or in urea excretion. Blood pH remains constant, and plasma total CO2, PCO 2, HCO3 -. total ammonia and NH3 concentrations all rise significantly. Exposure to 923 μmol/l NH4Cl does not elicit an increase in urea production or airbreathing. Aquatic hypoxia without access to air does not cause a reduction in aerobic metabolism, and moderate levels result in death. These results suggest that Amia are incapable of aestivation, due to an inability to detoxify ammonia to urea and reduce metabolism, and die following three to five days of air exposure.  相似文献   

20.
The various components of the nitrogen budget of white steenbras,Lithognathus lithognathus Cuvier (Sparidae), were determined under experimental culture conditions. A simple closed system respirometer was used where experiments were conducted to investigate faecal prodution, nitrogen excretion rates and absorption efficiencies at 16C and 20C. Faecal loss, as a percentage of the ingested nitrogen, amounted to 3.59%. Gross, carbon and nitrogen absorption efficiencies ranged from 8 6 to 9 8 % and were not significantly affected by fish size, ration size or temperature (P < 0.05). Net protein utilization (NPU) and the biological value (BV) ranged from 78 to95%. Ammonia and urea constituted 77.93 and 10.07%, respectively, of the total exogenous nitrogen excretion, the balance being made up of other dissolved organic nitrogen (DON). Endogenous ammonia excretion rates were significantly greater in small fish compared to large fish (P < 0.05). Total non-faecal losses as a percentage of the ingested ration amounted to 21.86%. Fish size and ration size increased nitrogen excretion, but temperature had no effect. Maintenance rations were 7.55 mg g-1 dry weight day-1 (0.76%dry body weight) and 5.99 mgg-1 (0.60% dry body weight) for small and large fish, respectively. The high absorption efficiencies and high nitrogen indicated that white steenbras utilize the commercial trout pellet diet very efficiently.  相似文献   

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