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1.
京郊大蒜病毒病的研究及其鳞茎中病毒的脱除   总被引：8，自引：0，他引：8  
 京郊大蒜病毒病发生普遍,主要症状为条纹花叶、矮化和叶片扭曲畸形。病体细胞中含大量线状病毒粒体和风轮状内含物。线状病毒粒体长度范围为250-1875nm,以长度550-800nm粒体居多。其中长700-800nm的粒体被鉴定为大蒜花叶病毒(GMV),回接脱毒大蒜叶片产生条纹花叶症状。血清学鉴定表明GMV与洋葱黄矮病毒(OYDV)有近缘关系。长度500-600nm的粒体可能为大蒜潜隐病毒(GLV),回接脱毒大蒜不产生花叶症状。下述两种病毒为京郊大蒜的主要病毒。此外,个别标样含烟草花叶病毒,但不是大蒜的主要病毒。对800nm以下线状病毒粒体归属尚待研究。
应用营养茎尖、生殖茎尖和根尖分生组织培养技术,可以脱去上述主要病毒,获得脱毒大蒜。  相似文献   

2.
番茄斑萎病毒Tomato spotted wilt tospovirus(TSWV)是严重危害世界经济作物的一种病毒,寄主范围广泛。我们在研究中发现番茄斑萎病毒能侵染我国大蒜Allium sativum L.。利用摩擦接种法将TSWV接种到健康大蒜上,结果显示:接种14 d后大蒜新生叶片出现褪绿和白色斑点症状。ELISA检测显示大蒜叶片汁液与TSWV的单克隆抗体产生血清学反应,采用TSWV N基因的引物对大蒜叶片总RNA进行RT-PCR,结果扩增出约800 bp的条带,在NCBI上BLAST显示与TSWV YN5576的同源性最高,为98.52%。这些数据表明番茄斑萎病毒系统侵染我国大蒜。  相似文献   

3.
An unusual virus was isolated from a Japanese Cucumis melo cv. Prince melon plant showing mild mottling of the leaves. The virus had a broad experimental host range including at least 19 plant species in five families, with most infected plants showing no symptoms on inoculated and uninoculated systemically infected leaves. The virus particles were spherical, approximately 28 nm in diameter, and the coat protein (CP) had an apparent molecular mass of about 55 kDa. The virus possessed a bi-partite genome with two RNA species, of approximately 8,000 and 4,000 nucleotides. Both genome components for the new virus were sequenced. Amino acid sequence identities in CP between the new virus and previously characterized nepoviruses were found to be low (less than 27%); however, in phylogenetic reconstructions the closest relationship was revealed between the new virus and subgroup A nepoviruses. These results suggest that the new virus represents a novel member of the genus Nepovirus. A new name, Melon mild mottle virus, has been proposed for this new virus.  相似文献   

4.
线茸毒蛾(Dasychira grotei Moore),在福建南平、三明一带为害法国梧桐、重阳木、月季、樟树等植物。1984年9～10月室内饲养线茸毒蛾幼虫时,发现罹病致死,死虫腹足紧贴叶片或培养皿上。同时,在林间、行道树上也发现病死幼虫,呈典型的病毒症状,经鉴定是一种质型多角体病毒(CPV),本病毒在国内外均未见报道,为我们首先分离获得。  相似文献   

5.
Efforts to control viral diseases of grapevine include the production of certified material and development of virus-resistant transgenic grapevines. However, effective antiviral agents, once the viruses have infected the plants, are still lacking. This study shows that a crude garlic extract has significant antiviral activity against grapevine viruses. Replication of grapevine leafroll-associated virus 2 (GLRaV-2) was obviously inhibited in grapevine cv. Cabernet Sauvignon calli treated with diluted (1:100) garlic extract. The relative RNA levels of GLRaV-2 and grapevine fleck virus (GFkV) in cv. Summer Black grapevine in in vitro-grown plantlets 10 days after treatment with diluted (1:100) garlic extract were about 22% and 20%, respectively, of that in controls. The viral RNA accumulation of GLRaV-2, GFkV, grapevine virus A (GVA), grapevine fanleaf virus (GFLV) and grapevine rupestris stem pitting-associated virus (GRSPaV) in field-grown grapevine cv. Centennial Seedless plants sprayed with diluted (1:100) garlic extract were about 31–40%, 26–38%, 18–31%, 17–42% and 15–18%, respectively, of that in controls. Moreover, the garlic extract treatment led to a significant decrease in viral RNA accumulation of GLRaV-3, GLRaV-2, GVA, GFkV, GFLV, GRSPaV and grapevine Pinot Gris virus in pot-grown grapevine cv. Shine Muscat plants, and viral disease symptoms in these plants were obviously attenuated. In addition, this extract significantly induced expression of pathogenesis-related protein genes and stimulated activity of antioxidant enzymes in grapevines. Taken together, these results indicate that the crude garlic extract acts as a significant inhibitor against a broad range of grapevine viruses.  相似文献   

6.
A potyvirus was isolated from Datura stramonium, Lycopersicon esculentum (tomato) and Solanum nigrum in the Yemen. It was transmitted mechanically and by Myzus persicae in a non-persistent manner. Its flexuous rod-shaped particles had a mean length of 719 nm and some of its pinwheel inclusion bodies in infected Nicotiana clevelandii leaves were unusual in that they were dichotomously branched. The virus infected various solanaceous species, but the symptoms it induced were distinct from those of pepper veinal mottle (PVMV) and potato Y viruses. Its particles were purified from N. glutinosa and their coat protein had an atypically high molecular mass a potyvirus of 41·5 kDa. They showed a distant serological relationship to those of PVMV and potato virus V in ISEM decoration tests, but did not react with antisera to particles of any other potyvirus tested. The virus has been tentatively named tomato mild mottle virus.  相似文献   

7.
Mechanical inoculation tests and ELISA with sap from garlic plants used for sanitation by meristem-tip culture revealed four viruses, viz. garlic common latent virus (GCLV) (carlavirus), the garlic strains of leek yellow stripe virus (LYSV-G), onion yellow dwarf virus (OYDV-G) (aphid-borne potyviruses), and onion mite-borne latent virus (OMbLV-G) (taxonomically unassigned virus). The same tests performed on explants grownin vitro showed elimination efficiencies of 100% for LYSV-G, 92% for OYDV-G, 62% for GCLV, and less then 54% for OMbLV-G.Meristem tips excised from garlic cloves and bulbils, 0.15–1.0 mm in size, were tested for regeneration and efficiency of virus elimination after transfer to Murashige and Skoog medium. Successful regeneration into plantlets was obtained with 71% of the meristems from cloves and 72% of those from bulbils, but virus elimination was easiest from cloves: 38% of all explants from cloves and 25% of those from bulbils were virus-free. The efficiency of elimination increased with increasing weight of the cloves, irrespective of the virus. Small tip size seemed to favour virus elimination, but sizes smaller than 0.4 mm led to increasing failure of regeneration.Micropropagation was most successful when cytokinins were omitted from the medium and the garlic shoot was split. Multiplication factors of 3–6 were obtained.  相似文献   

8.
Nearly 5700 plants of 14 cultivated and 8 wildAllium species and varieties from the Netherlands and other parts of the world, were tested for infection with aphid-borne potyviruses by ELISA, electron microscope decoration tests and/or inoculation onto test plants. This resulted in the detection of two known viruses, viz. leek yellow stripe virus (LYSV) and onion yellow dwarf virus (OYDV), and the discovery and characterization of two new viruses, viz. shallot yellow stripe virus (SYSV) and Welsh onion yellow stripe virus (WoYSV), and of six strains of these viruses. ‘Garlic mosaic’, ‘barlic yellow streak’, ‘onion mosaic’, ‘shallot mosaic’, ‘shallot X’, and ‘shallot yellows’ viruses, incompletely described in the literature, are now reidentified as well-known viruses or as strains or mixtures of such viruses. ‘Garlic yellow stripe virus’ is also a complex containing a potyvirus possibly differing from the viruses found in this survey. The symptoms of the potyviruses studied varied widely and ranged from mild to severe chlorotic to yellow striping of leaves, and they are of little diagnostic importance.LYSV was found in vegetatively propagated pearl onion (A. ampeloprasum var.sectivum) from Europe and Asia. It has decreased in leek crops (A. ampeloprasum var.porrum) in the Netherlands since the 1970, apparently due to resistance in new cultivars. OYDV was common in onion (A. cepa var.cepa) from the former USSR and North Africa, and in European cultivars of shallot (A. cepa var.ascalonicum), with the exception of the highly resistant ‘Santé’, but was not detected during this survey in Asian shallot. European samples of ever-ready onion (A. cepa var.perutile), multiplier onion (A. cepa var.aggregatum) and tree onion (A. cepa var.viviparum) contained OYDV. It was also found in sand leek (A. scorodoprasum) from european gene collections. A strain of OYDV from onion and shallot in Morocco and Spain was virulent on onion and shallot cultivars resistant to common OYDV, as reported early for a similar isolate in the USA.Asian shallot appeared generally infected with the new SYSV, similar to OYDV in host range and symptoms but serologically distinct. It was not detected in onion and shallot from Europe or North Africa. A virulent strain of this virus caused striping in sap-inoculated garlic (A. sativum) and Formosan lily (Lilium formosanum). The new WoYSV, infecting Welsh onion in Indonesia and Japan, was earlier described in Japan as OYDV from rakkyo and Welsh onion. It appeared serologically closely related to SYSV and distantly to OYDV, but differed in its host range.Host-specific strains of LYSV and OYDV were detected in garlic, wild garlic (A. longicuspis), an unidentifiedAllium species (suffix-G), and great-headed garlic (A. ampeloprasum var.holmense) (suffix-GhG)., LYSV-G and OYDV-G infected on average 45% and 73%, respectively, of the garlic samples of worldwide origin. Symptoms of isolates of both strains varied in severity, implying the necessity of serological tests for disease diagnosis and health certification. LYSV-GhG was the cause of yellow striping in 93% of the great-headed garlic plants tested, mainly from the Mediterranean area. One sample was also infected with OYDV-GhG.Many samples from vegetatively propagated crops grown from non-certified planting stock contained a few plants free of potyviruses, implying the possibility to obtain healthy (and possibly resistant) selections of such cultivars avoiding meristem-tip culture. Cross-protection of garlic sets by a mild potyvirus isolate seems to be an alternative to the use of vulnerable virus-free sets.Generally, viruses and virus strains could not be transmitted to anyAllium species other than their natural host, except to the highly susceptible crow garlic (A. vineale). This species, and other predominantly vegetatively propagating wildAllium spp. (field garlic,A. oleraceum; ramsons,A. ursinum; sand leek), were found not to be reservoirs of viruses that might infectAllium crops in the netherlands. Streaking in vegetatively propagated wild leeks (A. ampeloprasum and closely related species) originating from the Mediterranean area and Asia was due to an undescribed miteborne virus. The survey confirmed that spread of potyviruses inAllium crops in the Netherlands is from planting sets, and from a neighbouring crop only if of the same species.  相似文献   

9.
Carlavirus isolates from cultivated Allium species represent three viruses   总被引：3，自引：0，他引：3  
From 12 cultivated and mostly vegetatively propagatedAllium species and varieties tested for carlavirus infections, 94 virus isolates were obtained which varied greatly on indicator hosts.Chenopodium amaranticolor, C. quinoa, Celosia argentea var.plumosa Geisha,Nicotiana hesperis accession 67A andN. occidentalis accession P1 proved valuable for detection, isolation and propagation of part of the isolates. The latter three species are new experimental hosts for carlaviruses ofAllium species. Other isolates could only be transmitted toAllium species such as crow garlic (A. vineale) leek (A. ampeloprasum var.porrum) and onion (A. cepa var.cepa). The isolates were grouped into three viruses by differential hosts and host reactions and their reaction with four antisera.Shallot latent virus (SLV) was found in ever-ready onion (A. cepa var.perutile), grey shallot (unidentifiedAllium species), multiplier onion (A. cepa var.aggregatum), pearl onion (A. ampeloprasum var.sectivum), rakkyo (A. chinense), shallot (A. cepa var.ascalonicum), and Welsh onion (A. fistulosum). Virus isolates from garlic and Asian shallot, fully reacting with antiserum to SLV but differing in host reactions from the SLV type-isolate, are now described as garlic strain (SLV-G) and Asian shallot strain of the virus, respectively. The garlic latent virus from garlic described in Japan is now considered identical with SLV-G.A carlavirus almost universal in garlic, and also found in great-headed garlic (A. ampeloprasum var.holmense), in an unidentifiedAllium species, and occasionally in leek, did not react with the antisera to SLV and the Japanese garlic latent virus, and is now described as the new garlic common latent virus (GCLV). It appeared identical to a virus erroneously identified in Germany as garlic latent virus.The new Sint-Jan's-onion latent virus (SjoLV) from Utrechtse Sint-Jan's onion (unidentifiedAllium species) from the Netherlands and similar crops originating from other countries, did not induce reactions in test plants and could only be detected by electron microscope decoration tests. It reacted equally wellwith the antisera to SLV and GCLV. It was also present together with SLV in ever-ready onion, pearl onion, rakkyo, shallot, and Welsh onion. Garlic latent virus reported in Japan from hosts other than garlic should be regarded as SLV, SjoLV, or a mixture of these viruses.The carlaviruses were not detected in wild plants of ramsons (A. ursinum), and of the predominantly vegetatively propagated crow garlic (A. vineale), field garlic (A. oleraceum), and sand leek (A. scorodoprasum), collected in the Netherlands.Severe reactions in the indicator hosts incidentally revealed soil-borne viruses in shallot (the nepovirusesArabis mosaic virus (ArMV) and tomato black ring virus) and crow garlic (ArMV and the tobravirus tobacco rattle virus). Tobacco necrosis virus (necrovirus) was detected in roots of shallot.  相似文献   

10.
ABSTRACT Apple latent spherical virus (ALSV) expressing yellow and cyan fluorescent proteins (ALSV-YFP and ALSV-CFP) was used to investigate the distribution of identical virus populations in coinfected plants. In Chenopodium quinoa plants inoculated with a mixture of ALSV-YFP and ALSV-CFP, fluorescence from YFP and CFP was always distributed separately in both inoculated and upper uninoculated leaves. Inoculation of each ALSV-YFP and ALSV-CFP to different leaves of a C. quinoa plant resulted in the separate distribution of each virus population among different upper leaves. When C. quinoa leaves were first inoculated with ALSV-CFP and then ALSV-YFP was reinoculated into the same leaves at various times after the first inoculation, ALSV-YFP infected only tissues where ALSV-CFP infection had not been established. The spatial separation was also found in Nicotiana benthamiana leaves coinoculated with Bean yellow mosaic virus (BYMV)-YFP and BYMV-CFP. In contrast, both YFP and CFP fluorescence signals were observed in the same tissues of N. benthamiana leaves mixed infected with ALSV-YFP and BYMV-CFP. YFP fluorescence from ALSV-YFP in mixed-infected leaves was brighter and longer than in leaves infected with ALSV-YFP singly.  相似文献   

11.
A potyvirus causing severe mosaic symptoms was isolated from Senna occidentalis (syn. Cassia occidentalis ) in the Yemen Republic and Ethiopia. It was transmitted mechanically and by Myzus persicae in a non-persistent manner. The flexuous, rod-shaped particles had a mean length of 830 nm, and pinwheels and scrolls were observed by electron microscopy of thin sections of infected Nicotiana clevelandii leaves. Its host range was narrow with only a few legume species, Nicotiana clevelandii and N. benthamiana susceptible to experimental infection. This virus was purified from N. clevelandii and the coat protein had a molecular mass of 34-5 kDa. It reacted positively in ELISA with monoclonal antibody 197 that is specific for potyviruses, but was not decorated by antibodies to any other potyvirus tested when examined by electron microscopy. The virus has been tentatively named cassia severe mosaic potyvirus.  相似文献   

12.
A new strain of Apple stem grooving virus (ASGV) has been identified in Actinidia chinensis imported from China. The leaves of these plants exhibited a variety of symptoms including interveinal mottling, chlorotic mosaics and ringspots. Capillovirus-like particles were observed under the electron microscope, and the virus could be mechanically transmitted to a range of herbaceous indicators. The virus was detected using ELISA with antisera raised against ASGV. Sequencing of the virus revealed that it had more than 95% amino acid identity with ASGV in the putative coat and movement proteins. From the morphological, transmission, serological and molecular evidence, it was concluded that the virus is a strain of ASGV. It is not known how this strain of ASGV is transmitted, other than by grafting, nor is it known what effect the virus has on the growth of infected vines. The Actinidia -infecting strain of ASGV does not occur in New Zealand, and infected plants will not be released from quarantine. The detection methods used during the research will assist quarantine and the safe movement of breeding material.  相似文献   

13.
Onion yellow dwarf virus (OYDV) was detected in cloves and aerial bulblets of garlic (Allium sativum) at levels as high as or higher than in leaves of plants grown from tested cloves. It is recommended to test bulblets or a few cloves per bulb before planting to determine if all cloves of a bulb are virus-free. This aids in early detection and allows a more thorough testing of stock than field testing.  相似文献   

14.
While testing several samples of onion and of vegetatively propagated garlic, sand leek and shallot from a number of countries, virus isolates with unusually flexuous particles were obtained by mite (Aceria tulipae) or sap transmissions. No aphid-borne poty-or carlavirus was transmitted by mites, and mite-borne virus isolates could not be transmitted by aphids. The mite-borne isolates did not react with antisera to aphid-borne potyviruses ofAllium spp. or with the Agdia potyvirus group monoclonal. In contrast to the mite-borne onion and garlic mosaic viruses reported in the literature, our mite-borne isolates induced no visible or only very mild symptoms inAllium spp., except isolates from shallot ‘Santé’ which caused diffuse striping. Heavily mite-infested test plants or plant samples showed streaking and malformation due to mite feeding (tangle-top). The mite-borne virus isolates could be classified with test plants and a discriminating antiserum into three groups, representing two viruses and a strain of one of them. They are tentatively named onion mite-borne latent virus (OMbLV), garlic strain of this virus (OMbLV-G), and shallot mite-borne latent virus (SMbLV). Mite transmission, length of virus particles (ca. 700 to 800 nm), and the presence of granular inclusion bodies in infected tissue indicate that the viruses belong to the mite-borne genusRymovirus of the familyPotyviridae. OMbLV from shallot and onion, and OMbLV-G from garlic and sand leek, can be assayed onChenopodium murale but differ in their natural hosts. They are very common. SMbLV, to whichC. murale does not react, was isolated from shallot originating from Asia and Russia.  相似文献   

15.
 在1985年4月蚕豆病毒病的调查中,先后在四川、湖北、江苏和浙江4省的秋播蚕豆试验区内都发现一种由种子带毒的蚕豆染色病毒(BBSV)。所有病株都发生在由叙利亚国际旱地农业研究中心供种的试验区内,约10-40%的小区或品系发病,株发病率在0-18%之间。病害是随种子传入我国的。
病株为系统感染,病株叶片上的症状可以是轻花叶、斑驳、褪色斑或畸形,有的小叶正常而无明显病变。病毒粒体为等径球状体,直径28毫微米。病毒样本能与英g国染色病毒的抗血清发生沉淀反应,但与真花叶病毒的抗血清不发生反应。在病株上未发现能传毒的象甲,也无象甲的为害状,病毒病尚未扩散。在试验区以外的农田里未发现有蚕豆染色病毒存在。  相似文献   

16.
A soilborne disease of lettuce, associated with necrosis and dieback, has been found with increasing frequency in California and Arizona over the last 10 years. An isometric virus, serologically related to Tomato bushy stunt virus (TBSV), was consistently isolated from lettuce plants with these disease symptoms. Back-inoculation to healthy lettuce plants and subsequent reisolation of the virus from symptomatic lettuce leaves suggested that this virus was the causal agent of this disease. A tombusvirus was also associated with a necrosis disease of greenhouse-grown tomatoes in Colorado and New Mexico. Complementary DNA representing the 3' end of viral genomic RNAs recovered from diseased lettuce and tomato plants had identical nucleotide sequences. However, these sequences were divergent (12.2 to 17.1%) from sequences of the previously described strains of TBSV, Petunia asteroid mosaic virus (PAMV), Artichoke mottled crinkle virus, and Carnation Italian ringspot virus. Additional tombusvirus isolates were recovered from diseased lettuce and tomato plants and these were most closely related to the TBSV-cherry strain (synonymous with PAMV) and to Cucumber necrosis virus based on comparison of 3'-end sequences (0.1 to 0.6% and 4.8 to 5.1% divergence, respectively). Western blot analysis revealed that the new tombusvirus isolated from diseased lettuce and tomato plants in the western United States is serologically distinct from previously described tombusvirus species and strains. Based on genomic and serological properties, we propose to classify this virus as a new tombusvirus species and name it Lettuce necrotic stunt virus.  相似文献   

17.
Surveys to identify virus diseases affecting garlic ( Allium sativum ), onion ( Allium cepa ) and Persian leek ( Allium ampeloprasum var. persicum ) were conducted from 1999 to 2002. Surveys covered different regions of Iran (Tehran [different vegetable markets, farmer fields and cultivation areas], Noushahr, Chalous, Roudbar, Sari, Hamadan, Touyserkan, Ghazvin and Jiroft). A total of 2045 (1285 garlic, 525 onion and 230 leek) samples showing symptoms of virus infection were collected and tested by ELISA; and in some cases tests were also confirmed by immunoelectron microscopy (IEM) for the presence of Allium viruses. ELISA results showed that the following viruses were detected: Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV) (genus Potyvirus , family Potyviridae ), Garlic common latent virus (GarCLV), Shallot latent virus (SLV) (genus Carlavirus ), Garlic virus D (GarV-D), Garlic virus B (GarV-B) and Garlic virus C type (GarV-C) (genus Allexivirus ). None of the samples reacted with antibodies to Shallot yellow stripe virus (SYSV) genus Potyvirus , family Potyviridae ), Shallot virus X (ShVX) and Garlic virus A (GarV-A, genus Allexivirus ). GarCLV, SLV, GarV-D, GarV-B and GarV-C are reported for the first time from Allium crops in Iran.  相似文献   

18.
A-蛋白和羊抗兔血清应用于免疫电镜,检测马铃薯X病毒(PVX)粗汁液,灵敏度比诱捕修饰法高;而且加羊抗兔血清后,病毒粒体比诱捕修饰法明显加粗,在电镜放大2500倍时,就能清晰地观察到。用诱捕双修饰法检测PVX,证实在寄主植物的叶、茎和根中均存在,以叶内含量最高。接种在普通烟上第三天,接种叶就能检测到PVX,接种15天后,寄主体内病毒能达到较高浓度,而且高浓度一直可保持2个月以上;PVX在不同寄主中的含量不同,以普通烟中病毒浓度最高,心叶烟、番茄和黄花烟中次之;昆诺阿藜、千日红和大椒中含毒量极抵。用诱捕双修饰法检测马铃薯薯块休眠芽中的病毒获得成功。  相似文献   

19.
ABSTRACT In the late 1990s, commercial garlic fields in California (CA) were devastated by an outbreak of rust caused by Puccinia allii. We compared collections of the pathogen from garlic (Allium sativum) and chives (A. schoenoprasum) in central CA and Oregon (OR) to collections from garlic and leek (A. porrum and A. ampeloprasum) in the Middle East. Teliospores from the CA and OR collections were smaller in length, width, and projected cross-sectional area compared with collections from the Middle East. CA and OR collections had a shortened life cycle, in which pycnia and aecia were not formed. Germinating teliospores produced a two-celled promycelium, resulting in two basidiospores, each initially with two nuclei, indicating that this rust was homothallic. In addition, the morphology of the substomatal vesicles was different between the CA-OR (fusiform) and the Middle Eastern (bulbous) collections. DNA sequence analysis of the nuclear ribosomal internal transcribed spacer region showed that the CA and OR rust collections formed a well-supported cluster distinct from the Middle Eastern and European samples. These results suggest that the rust on garlic and chives in CA and OR is a different species than the rust fungus on garlic and leek in the Middle East.  相似文献   

20.
2019年山东种植的西葫芦上广泛发生病毒病,症状与之前常发症状有差异,发病植株叶片向下卷曲、黄化,植株矮化。为明确引起此次西葫芦病毒病的病原,我们以田间采集的10份西葫芦病叶为材料,进行PCR扩增并测序。测序结果显示扩增片段核苷酸序列与我国广东的中国南瓜曲叶病毒(SLCCNV)南瓜分离物(MW389917.1)一致性最高。根据同源序列设计引物,经PCR扩增获得SLCCNV全长序列,DNA-A 全长为2 730 bp(OM692270.1)、DNA-B 全长为2 711 bp(OM692269.1),经序列比对发现DNA-A序列与已登录的SLCCNV一致性为89.65%~99.42%,其中与我国广东的SLCCNV-GDHY南瓜分离物(MW389917.1)一致性最高,为99.42%;DNA-B序列与已登录的SLCCNV一致性范围为81.82%~97.29%,其中与我国广东的SLCCNV-GDHY南瓜分离物(MW389918.1)一致性最高,为97.29%。因此推测引发山东西葫芦病毒病的病原物是SLCCNV,由于该病毒是在山东西葫芦上首次发现,将其命名为SLCCNV-SD。前人已报道SLCCNV可侵染南瓜、甜瓜、烟草、番茄等作物,但SLCCNV可侵染西葫芦在国内未见报道。  相似文献   

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