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犀牛产气荚膜梭菌的分离鉴定 总被引：1，自引：1，他引：0
从突然死亡的犀牛肠管中，分离到一株套氧菌，经鉴定为致病性Ａ型产气荚膜梭菌。该菌为革兰氏阳性大杆菌，在血平皿套氧培养，产生双环溶血的菌落，用产毒培养基培养，其上清液静脉注射０．２ｍｌ可致死小白鼠。证明该菌是造成犀牛死亡的病原。血清中和试验结果中Ａ型产气荚膜梭菌，该分离物鉴定为致病性的Ａ型产气荚膜梭菌。 相似文献
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多重聚合酶链反应检测环境中产气荚膜杆菌 总被引：12，自引：0，他引：12
产气荚膜杆菌根据产生4种主要毒素（α－β、ε、τ－毒素）可分为5种类型A－E型。在该研究中依据产气荚膜杆菌的5种毒素基因的序列，设计了5对PCR引物，建立了多重PCR方法，该方法可以快速区分5种类型的产气荚膜杆菌。并对68份环境样品（生活污水、生物肥料）进行检测和基因分型，共检出55株产气荚膜杆菌，其中A型产气荚膜杆菌50株，占总数的90％以上，B型、C型、D型只占5％，未检出E型产气荚膜杆菌，所有的分离株没有发现带有肠毒素。结果表明，目前环境中主要存在的菌型为A型菌，其他菌型的产气荚膜杆菌很少，而多数是非致病性产气荚膜杆菌。 相似文献
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非洲狮产气荚膜梭菌病的病原检测 总被引：1，自引：0，他引：1
北京动物园一头非洲狮突然死亡,死前曾有神经症状,死后立即解剖,主要病变为肺出血,十二指肠出血。从肺和肾组织分离到Ａ型产气荚膜梭菌,用该菌液０．０５ｍｌ腹腔注射小白鼠致死,用该菌的肉肝胃酶消化汤５ｈ培养的上清液０．１ｍｌ静脉注射小白鼠６ｈ死亡。产气膜梭菌除对草食动物有较强致病作用外,对大型猫科动物也有致病性。 相似文献
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无菌采取内蒙古通辽市某羊场病死羊肠道内容物、肝脏和肺脏,进行细菌的分离培养。将从十二指肠内分离到的1株疑似致病菌株进行生化试验、小鼠致病性试验,再将其通过魏氏梭菌多重PCR试验、魏氏梭菌ELISA试验及16S rRNA PCR试验进行鉴定。将PCR产物进行测序并进行了16S rRNA基因的进化树分析。结果显示,经细菌生化试验、多重PCR试验、ELISA试验和16S rRNA试验均证实此分离株为A型产气荚膜梭菌;进化树分析显示该菌与序列号为HQ808749.1(美国)的A型产气荚膜梭菌遗传距离最近。结果表明,该羊病例所分离的致病菌为A型产气荚膜梭菌。 相似文献
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产气荚膜梭状芽胞杆菌 (Ｃｌｏｓｔｒｉｄｉｕｍｐｅｒｆｒｉｎｇｅｎｓ)简称产气荚膜梭菌 ,又称魏氏梭菌 (Ｃｌｏｓｔｒｄｉｕｍｗｅｌｃｈｉｉ) ,根据该菌产生的α、β、ε、ι 4种主要毒素 ,将其分为Ａ、Ｂ、Ｃ、Ｄ、Ｅ 5型。1 994年笔者报道了国外由Ａ、Ｂ、Ｃ型菌感染或混合感染所致仔猪坏死性肠炎的猝死症。近年国内也时有由产气荚膜梭菌Ａ、Ｂ、Ｃ、Ｄ、Ｅ五型菌所致多种家畜猝死症的报道。现将产气荚膜梭菌所致疫病、所产毒素及近年国内由产气荚膜梭菌所致家畜猝死症概述如下。1 产气荚膜梭菌所致疫病及所产毒素类型毒素中和试验对… 相似文献
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《中国兽医学报》2020,(1):129-134
产气荚膜梭菌是一种人兽共患病原菌,可通过多种途径感染人类。本研究从迁徙候鸟样品中对产气荚膜梭菌进行分离鉴定,并进行毒力基因检测、分型、生物被膜形成能力鉴定及耐药性测定。结果从549份健康候鸟粪便样品中分离细菌42株,35只死亡候鸟病料样品中分离细菌11株,通过PCR方法共鉴定A型菌48株,E型菌5株,所有菌株均未检出β2毒素、肠毒素和NetB毒素基因,30株经测定能够形成生物膜;分离菌株对黏菌素、杆菌肽、克林霉素和红霉素的耐药率在40%以上,所有菌株均对复方新诺明敏感。结论得出迁徙候鸟携带产气荚膜梭菌的比例较低,但是对部分抗生素的高耐药性值得关注。 相似文献
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《中国兽医杂志》2016,(5)
通过对死于出血性肠炎的圈养鹿的病原菌进行分离鉴定,为研制产气荚膜梭菌β-毒素单价和多价疫苗奠定基础。采集山西省内不同地区鹿场因出血性肠炎而死亡鹿的病料32例,经病原微生物分离培养、生化试验和血清型鉴定,分离得到C型产气荚膜梭菌,并测定分离菌所产毒素对小鼠的最小致死量。PCR扩增C型产气荚膜梭菌β-毒素基因,构建重组质粒p MD18-T-J28-C,进行酶切鉴定和核苷酸序列分析。结果 32株分离菌中有6株是C型产气荚膜梭菌,占18.7%;其余均为A型,占81.3%。筛选出毒力最强的菌株J28-C,最小致死量(MLD)为5.0×105CFU/m L。PCR扩增和核苷酸序列分析表明,经PCR得到了特异性的β毒素基因片段。表明造成山西省鹿出血性肠炎的病原菌为A型和C型产气荚膜梭菌,以A型为主。 相似文献
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Mayor D Korczak BM Christensen H Bisgaard M Frey J Kuhnert P 《Veterinary microbiology》2006,116(1-3):194-201
Strains of [Actinobacillus] rossii, [Pasteurella] mairii and [Pasteurella] aerogenes can be isolated from abortion in swine. The RTX toxin Pax has previously been found only in those [P.] aerogenes strains isolated from abortion. Nothing is known about RTX toxins in field isolates of the other two species. To gain insight into the distribution of selected RTX toxin genes and their association with abortion, PCR screening for the pax, apxII and apxIII operons on 21 [A.] rossii and seven [P.] mairii isolates was done. Since species can be phenotypically misidentified, the study was backed up by a phylogenetic analysis of all strains based on 16S rRNA, rpoB and infB genes. The pax gene was detected in all [P.] mairii but not in [A.] rossii strains. No apx genes were found in [P.] mairii but different gene combinations for apx were detected in [A.] rossii strains. Most of these strains were positive for apxIII, either alone or in combination with apxII. Whereas pax was found to be associated to strains from abortion no such indication could be found with apx in [A.] rossii strains. Phylogenetically [A.] rossii strains formed a heterogeneous cluster separated from Actinobacillus sensu stricto. [P.] mairii strains clustered with [P.] aerogenes but forming a separate branch. The fact that [P.] aerogenes, [P.] mairii and [A.] rossii can phylogenetically clearly be identified and might contain distinct RTX toxin genes allows their proper diagnosis and will further help to investigate their role as pathogens. 相似文献
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Three strains of the Pasteurella aerogenes complex were isolated as sole pathogens from aborted fetuses of a sow aborted at the 12th week of gestation on a farm of 600 sows. Gross pathology showed no characteristic lesions. The isolates were biochemically identical and resembled P. pneumotropica on the basis of their strong indole and urease positivity but they produced gas, were ornithine decarboxylase negative and fermented mannitol but not trehalose. Only a few differences were apparent in biochemical characteristics between the isolated strains and P. aerogenes. They differed from the type strain of P. aerogenes in ornithine decarboxylase activity, indole production and lactose and mannitol fermentation; however, such strains do occur within this heterogeneous species. At the time of abortion the antibody titre of the aborted sow was 1 in 16 when examined with live bacterial suspension and 1 in 128 if boiled antigen was used. Similar strains could not be isolated from the vaginas of aborted sows or pregnant and newly farrowed sows in the same group. The bacteriological, serological and histological findings support the opinion of other workers on the occasional pathogenic nature of P. aerogenes. 相似文献
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Isolation,Identification and Pathogenicity Research of a Strain of Clostridium perfringens from Goat
A pathogenic bacteria was isolated from a dead goat,and it was identified as goat-pathogenic type A Clostridium perfringens by differential culture,biochemical test,molecular biological technology and animal pathogenicity test.Analysis results of toxin genes showed that this bacteria contained both α and β2 toxin genes.Animal pathogenicity test result showed that this bacteria was highly pathogenic to mice,and the same bacteria was isolated from dead mice as which was isolated from the dead goat,so it was certain that type A Clostridium perfringens was the main pathogenic bacteria which caused the goat dead.The results would provide scientific basis for the treatment and prevention of the disease caused by Clostridium perfringens in this goat farm. 相似文献
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H Hauke 《Archiv fuer experimentelle veterinaermedizin》1979,33(5):665-673
Isolated acholeplasma laidlawii strains exhibited highly differentiated behaviours regarding their udder pathogenicity. Twelve of 16 tested strains were pathogenic to udder. Symptoms of acute udder inflammation were caused by all ten A. laidlawii strains isolated from differentiated material of calf, but by only two of six strains isolated from differentiated material of cattle. Intracisternal instillation of both strains from milk and one strain each from udder skin or cervical mucus caused merely temporary disorders of secretion. Ultrasonic extracts of A. laidlawii strains, some of them additionally heated, were intracisternally applied, as well. Udder irritation was caused only by those acholeplasma strains which were udder-patha was assumed to be attributable to a toxin of the polysaccharide type. Pathogenicity to udder was recorded also from one M. alkalescens strain isolated from a nose swab taken of cattle as well as from two A. granularum strains isolated from calf lungs. 相似文献
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Actinobacillus suis-like organisms and evidence of hemolytic strains of Actinobacillus lignieresii in horses 总被引：1，自引：0，他引：1
Thirty-seven local isolates of Actinobacillus suis-like organisms from diseased and clinically normal horses and 1 llama were compared with reference strains of A suis, A lignieresii, A equuli, A capsulatus, A hominis, A (Pasteurella) ureae, and equine A suis-like organisms (ASLO) previously described in literature. Comparison was by cultural characteristics, carbohydrate fermentation, enzyme profiles, and whole-cell protein polyacrylamide gel electrophoresis. Carbohydrate fermentation, determined by API-CH gallery, divided 36 equine ASLO isolates into 6 API-CH biotypes. The llama isolate was an additional distinct biotype. The biochemical comparisons between A suis and ASLO did not reveal remarkable and consistent differences. Enzyme analysis revealed 5 API-ZYM biotypes, one of which included the same strains as one of the API-CH biotypes and consisted in both instances of 4 esculin-negative ASLO cultures and the reference strain of A lignieresii. We conclude that the 4 strains were hemolytic variants of A lignieresii. Protein electrophoresis disclosed 15 banding patterns, 10 of which represented equine ASLO strains. The reference strains of A suis shared the pattern predominant among equine ASLO. Four of the remaining reference strains of Actinobacillus species each had a unique profile, whereas the type strain of A capsulatus and the llama isolate had similar profiles. The groupings of cultures resulting from the different testing methods had little relation to each other and to the anatomic source of the strains except the strains comprising API-CH biotype II, which originated in the equine respiratory tract, and the A lignieressi cluster. 相似文献
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从发病仔猪脏器中分离到一株细菌,进行了初步鉴定。结果表明,该菌为致猪水肿病大肠杆菌,在血平板上呈β溶血,经PCR鉴定该菌株具有产毒素基因,对小鼠毒力强,能致其发病死亡,血清型为O141,是临床多发血清型菌株。将细菌培养物和裂解毒素分别经皮下和尾静脉两种途径接种于体重为16~18 g昆明系小鼠,小鼠腹腔注射细菌培养物后12~48 h死亡,不表现神经症状,剖检水肿病病变特征不典型;小鼠于尾静脉注射裂解毒素后4~48 h,出现典型的眼睑水肿和后躯瘫痪等神经症状,直至死亡。该菌株致小鼠死亡的最小毒素剂量为0.2 mL/只。研究结果表明,小鼠可作为诊断仔猪水肿病模型,也进一步证明试验菌株为致猪水肿病菌株,可作为制备疫苗菌株。 相似文献
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利用细菌培养、生化鉴定、小鼠致病性试验、PCR技术等方法对西藏那曲市多起牦牛猝死病例进行诊断和分析。结果表明,从病死牦牛脏器中分离得到3株产气荚膜梭菌,命名为AD-01、AD-02和BG-01。其中AD-01和AD-02分离株属于A型,BG-01分离株属于C型。通过对分离株16S rRNA进行序列比对并制作进化树分析发现,AD-01和AD-02分离株为A型产气荚膜梭菌,与中国近年来报道的分离菌株遗传距离较远,属于完全独立的一个分支;同时药敏试验结果显示AD-01、AD-02分离株与BG-01分离株的药敏试验结果有一定差异,产生此现象的原因需进一步研究。本试验成功分离到3株牦牛源产气荚膜梭菌,为牦牛产气荚膜梭菌病的预防和控制提供了理论依据。 相似文献
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Objective The objective of this work was to perform a comprehensive phenotypic characterisation of 16 isolates of bacteria previously identified as Actinobacillus equuli.
Design The 16 isolates that had been obtained from Australian animals – 15 from horses and one from a rabbit – were compared with reference strains of A equuli, A capsulatus, Pasteurella caballi and Bisgaard Taxa 9 and 11.
Results The characterisation study demonstrated that only nine of the isolates were A equuli . The other isolates were identified as A capsulatus (the isolate from rabbit), P caballi (one isolate), Bisgaard Taxon 11 (two isolates) and Bisgaard Taxon 9 (one isolate). The final two isolates could not be assigned to any recognised species or taxa.
Conclusion This study has highlighted the importance of a complete characterisation of Actinobacillus -like organisms isolated from horses and rabbits. The study represents the first time that A capsulatus, P caballi and Bisgaard Taxa 9 and 11 have been recognised as being present in Australia. 相似文献
Design The 16 isolates that had been obtained from Australian animals – 15 from horses and one from a rabbit – were compared with reference strains of A equuli, A capsulatus, Pasteurella caballi and Bisgaard Taxa 9 and 11.
Results The characterisation study demonstrated that only nine of the isolates were A equuli . The other isolates were identified as A capsulatus (the isolate from rabbit), P caballi (one isolate), Bisgaard Taxon 11 (two isolates) and Bisgaard Taxon 9 (one isolate). The final two isolates could not be assigned to any recognised species or taxa.
Conclusion This study has highlighted the importance of a complete characterisation of Actinobacillus -like organisms isolated from horses and rabbits. The study represents the first time that A capsulatus, P caballi and Bisgaard Taxa 9 and 11 have been recognised as being present in Australia. 相似文献