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1.
For two consecutive years, determinations were made of the prevalence and abundance of coccidian oocysts from crossbred beef calves raised on Bahia grass pastures in the coastal plain area of Georgia. Of the 534 fecal samples collected during the survey, 461 (86.3%) contained one or more species of coccidian oocysts. The total mean oocysts per gram of feces (OPG) was significantly different (P less than 0.10) between the two years. Thirteen species of Eimeria were found during both years of the survey. Eimeria bovis was the most prevalent species found in the survey; E. ellipsoidalis, however, had the greatest number of OPG. Eimeria bukidnonensis, E. pellita, and E. brasiliensis were the least prevalent species and also had the lowest numbers of OPG. Only one clinical case of coccidiosis was seen. A nine-month-old calf had E. zuernii coccidiosis; it excreted E. zuernii oocysts in several fecal samples before the clinical disease appeared.  相似文献   

2.
Fecal samples (2,019) from cattle in the university farm, Tohoku University, were examined for coccidian oocysts from April 1986 to January 1987, and 19.3% of them was positive for Eimeria spp. Thirteen Eimeria species were identified. Eimeria bovis (25.7%) was the most dominant species, followed by E. auburnensis (17.6%), E. canadensis (14.5%), E. alabamensis (9.7%), E. ellipsoidalis (8.1%), E. zuernii (7.0%), E. bukidnonensis (5.4%), E. brasiliensis (3.9%), E. cylindrica (1.3%), E. illinoiensis (0.4%), and E. pellita (0.2%). Fecal samples positive for coccidial oocysts amounted to 12.9% and 26.7% on average during grazing on pasture and loose housing, respectively.  相似文献   

3.
A field survey was carried out on prevalence of bovine coccidia in 9 prefectures of Japan in the autumn of 1985. A total of 1,015 fecal samples was obtained from dairy and beef cattle more than 2 weeks old. Coccidial oocysts were found in 59.0% of all the samples examined. There were no significant differences in the prevalence between dairy and beef cattle of the same age group. The prevalence was the highest in the animals between 6 and 11 months old and decreased to 25% in those more than 24 months old. In the majority of the samples positive for oocysts, OPG (the number of oocysts per gram of feces) was less than 200. No clinical coccidiosis was found except in a few cases. Eleven Eimeria and one Isospora species were identified. Of these species, E. bovis and E. ellipsoidalis were the most prevalent, followed by E. aubrunensis, E. brasiliensis, and E. cylindrica in all the prefectures. The other species identified were E. canadensis, E. alabamensis, E. zuernii, E. wyomingensis, E. bukidnonesis, E. subspherica, and Isospora sp. in the order of frequency. Isospora sp. will be a pseudoparasite caused by the contamination with feces of passerine birds.  相似文献   

4.
Information on the occurrence and diversity of coccidia infecting cattle in different farming systems in South Africa is lacking. The objective of the study was to determine the levels of infection of coccidia in cattle at three localities and to determine the Eimeria species occurring at each locality. Faecal samples (n=1936) were collected over a 13-month period from a dairy farm (Mallesons), a Bonsmara breeding farm (Kaalplaas) and a Nguni stud farm (Pienaars River). Samples were collected from adults >24 months old and calves <12 months old. The modified McMaster technique was used to determine OPG (oocysts per gram) of faeces. Samples with OPGs > or =2000 were allowed to sporulate in potassium dichromate solution at room temperature. Species identification was done by measurement and morphological characteristic of each species. At Mallesons, 29% of samples were positive, at Kaalplaas 50% and at Pienaars River 52%. The most prevalent species were Eimeria zuernii and E. ellipsoidalis at Mallesons, E. zuernii and E. bovis at Kaalplaas and E. bovis and E. zuernii at Pienaars River, respectively. Adults had very low OPGs. There was a pattern at the three localities in that high OPGs were only recorded in calves, which were also shedding the most pathogenic species.  相似文献   

5.
Coccidia in cows and calves on pasture in north Georgia (U.S.A.)   总被引:2,自引:0,他引:2  
The prevalence and abundance of coccidian oocysts were determined in a herd of beef cows and calves on fescue pastures in the Piedmont area of Georgia during 4-consecutive grazing seasons. Twelve species of Eimeria were found in the feces of the calves and 10 species were found in the feces of the cows. Eimeria bovis was the most prevalent species found in both the calves and cows. It occurred in 72.5% of 1090 samples from the calves and 10.2% of 719 samples from cows. Eimeria bovis also comprised the majority of oocysts present in the fecal samples from the calves and cows. The greatest number of E. bovis in a sample was 45 800 oocysts per gram of feces (OPG) from a calf and 1900 OPG from a cow. No cases of clinical coccidiosis were seen in any of the animals sampled during the survey.  相似文献   

6.
Clinical coccidiosis is associated with high fecal contamination and stress situations, mainly in animals under 1 year of age. Artificially fed dairy calves are one of the categories most prone to suffer from this parasitic disease. The study was carried out in a commercial dairy farm. Feces samples of heifer calves between 2 and 8 weeks old were taken monthly for oocyst counts and Eimeria spp. identification. Of the 862 feces samples analyzed, 48% presented oocysts. When grouping the results of monthly samplings of each age group, it was observed that this percentage increased in the group of calves between 20 and 40 days of life, reaching the peak average of 85% of infection prevalence in the group with between 26 and 30 days of age. The discharge of oocysts observed between 21 and 35 days of age was superior to the rest (p<0.05). This trend appeared every month throughout the whole year. However, during March, April, June, September and November, the curves in the group categories were higher than in the remaining months (p<0.05). Twelve Eimeria species were identified, being E. ellipsoidalis, E. bovis, E. zuernii and E. auburnensis those in highest numbers. E. ellipsoidalis had an important predominance in the opg composition, >75% up to 25 days of life (p<0.05). E. bovis reached peak values in the 26 and 30 days group (p<0.05), remaining without significant variations in the last stage of the artificial milk feeding period (approximately 60 days of life), when the oocyst counts were significantly low. Oocysts of E. auburnensis appeared in great proportion (46%) in the cultures later than the previous species (p<0.05), in calves of the age groups of between 46 and 50 days of age. Oocysts of E. zuernii showed no trend associated with age. The highest prevalence of infection and of oocyst values appeared during the periods with better environmental conditions for sporulation, survival and dispersion of oocysts (spring and autumn), coincident with the highest birth rates and an elevated number of calves in the paddock. Calves developed a process of natural "vaccination" against coccidiosis. This was demonstrated by the decrease in the quantity of animals shedding oocysts and in the number of oocysts eliminated at the end of the artificial milk feeding period.  相似文献   

7.
A longitudinal study was performed to establish estimates of prevalence of selected endoparasites in Swiss cow-calf operations and to investigate the importance of an infection with endoparasites on diarrhea and weight gain until weaning. Three hundred and eighty-six calves raised in 67 larger cow-calf herds were included in the study. Faecal samples were collected during the first three months of life and at weaning. Parasitological analyses were performed according to standard procedures including specifically the detection of Eimeria spp., Cryptosporidium parvum, Strongyloides papillosus and Trichostrongylida. The effects of an infection with endoparasites on weight gain were analyzed with a linear model accounting for effects of farm, breed, sex, calving month and weight at birth. The average prevalences of endoparasites within the first three months of life were: E. bovis 36%, E. zuernii, 19%, C. parvum 16.8%, S. papillosus 22.3% and Trichostrogylida 1.5%. The prevalence of diarrhea within the same time period was 13%. Prevalences at weaning (at the end of the grazing period) were: E. bovis 56.5%, E. zuernii 4.6%, C. parvum 3.7%, S. papillosus 14.4% and Trichostrogylida 84.5%. With the exception of E. zuernii, endoparasites were more frequently observed in healthy calves than in diarrheic calves. Weaning weights were available from 190 calves (33 herds). Statistical analyses of weaning weights revealed no evidence that an infection with helminths and/or protozoa within the first three months of life or at weaning had a negative influence on individual weight gain.  相似文献   

8.
Two experiments were conducted to evaluate lasalocid as a coccidiostat in Holstein calves and to compare lasalocid with monensin and decoquinate. In experiment 1, calves in 3 groups (6 calves/group) were each inoculated with 500,000 sporulated oocysts, 88% of which were Eimeria bovis and 12% were E zuernii. Calves in each group were given lasalocid-medicated feed at 0.50 (group 3), 0.75 (group 4), or 1 mg/kg (group 5) of body weight/day for 45 days. Two control groups (6 calves/group) were also evaluated; calves in control group 2 were inoculated and nontreated, and calves in control group 1 were noninoculated and nontreated. At 0.50, 0.75, or 1 mg/kg/day, lasalocid was equally effective in preventing induced coccidiosis (E bovis and E zuernii) in calves. Compared with inoculated nontreated controls, treated calves had significantly (P less than 0.05) fewer oocysts in feces and had fewer clinical signs of coccidiosis from days 16 to 30 after inoculation. Experiment 2 was conducted to compare the effectiveness of monensin, lasalocid, and decoquinate for the prevention of experimentally induced coccidiosis. Calves (n = 48) were allotted into 4 groups (12 calves/group); each was inoculated orally with 275,000 sporulated oocysts, predominantly E bovis and E zuernii, and each was given nonmedicated feed (group 6) or feed medicated with 33 mg of lasalocid (group 7), decoquinate (group 8), or monensin (group 9)/kg of feed for 46 days. Calves given medicated rations had significantly (P less than 0.05) fewer oocysts in their feces and fewer clinical signs of coccidiosis than did calves given nonmedicated rations.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

9.
Coccidia were detected in the faeces of 556 out of 592 sheep (93.9%) in sheep in Kars province. The prevalence of coccidiosis was significantly higher (p>0.01) in young (97.9%) and immature sheep (96.6%) than in adult sheep (90.2%). Mixed infections were widespread (71.5%). Ten species of Eimeria were recognized. These species (and their prevalences) were: Eimeria ovinoidalis (47.7%), E. bakuensis (E. ovina) (46.6%), E. parva (37.1%), E. granulosa (27.7%), E. ahsata (23.4%), E. pallida (23.2%), E. faurei (15.1%), E. intricata (13.9%), E. crandallis (13.7%) and E. punctata (2.3%). Eimeria punctata is reported in sheep from Turkey for the first time. The mean oocyst count from infected animals was 3451±409 opg (range 50–117 600). The mean was higher (9529±502) in young sheep. Differences in the counts from young, immature and adult sheep were statistically significant (p>0.01). However, no clinical cases of coccidiosis were encountered.  相似文献   

10.
The development of coccidial infections in 21 free-ranging, unweaned beef calves from birth to 8 months was investigated by examining faecal samples for oocysts. Most calves commenced shedding oocysts within a month of birth, and had shed all nine species identified by 3-4 months. Oocysts were shed by three calves as young as 12 and 13 days. The oocysts species shed earliest, and the commonest thereafter, were Eimeria bovis, E. ellipsoidalis and E. zuernii. Although several oocyst counts between 5.0 X 10(3) and 114.0 X 10(3)g-1 of faeces of these potentially pathogenic species were recorded there was no clinical disease. Some calves shed oocysts approximately four times more frequently than others, and individual species counts greater than or equal to 5.0 X 10(3)g-1 were confined to 10 of the calves. Intermittent oocyst shedding continued throughout the study with a similar distribution of oocyst species from all calves.  相似文献   

11.
Anticoccidial efficacy of dietary fat was evaluated in calves with coccidial infection (Eimeria spp., including E. bovis and E. zuernii). Medium-chain triglycerides (MCT)--natural edible fats composed of caprylic (C8), capric (C10), and lauric (C12) acids -- were given orally with milk to 5 calves and with 10% glucose solution to 3 older, weaned calves by using the reticular groove reflex. After 3 to 11 days of MCT feeding, all Eimeria spp. oocysts had disappeared from the feces of all calves. MCT had no adverse effects on appetite or on fecal pH, ammonia, lactic acid, or volatile fatty acid levels. MCT feeding for coccidial control in calves has minimal side-effects and has benefits in terms of residue-free food production.  相似文献   

12.
One- to six-month-old calves, housed in the large-capacity calf house of the Nová Bystrice state farm in the district of Jindrich?v Hradec were studied for the occurrence of coccidia in the period from January 1974 to April 1975. The coccidia found in these animals included nine species of the genus Eimeria: E. zuernii, E. bovis, E. ellipsoidalis, E. subspherica, E. auburnensis. The species E. alabamensis, E. cylindrica, E. wyomingensis and E. bukidnonensis occurred just sporadically. Further, the findings included oocysts of Isopora spp. The oocysts of the coccidia E. wyomingensis and Isopora spp. were found for the first time in Czechoslovakia. The overall extensity of invasion (EI) and intensity of invasion (II) grow with age as follows: (chart: see text) The results suggest that even modern large-capacity calf houses are not free from invasion by coccidia; the calves in the house under study were invaded by ten species. Transition to vegetable feeding is accompanied by a marked rise of the EI of all the species of coccidia found in the animals.  相似文献   

13.
Newborn Holstein male calves were purchased within 3 days after birth and were removed from the local farms to the Dixon Springs Agricultural Research Center. They were hand-fed for 7 weeks and then weaned to a prepared feed. Eight groups, each of 4 calves, were housed in separate pens. In each of 4 pens (pens 2 to 5), 1 calf was inoculated with sporulated oocysts of Eimeria bovis (and was not medicated); 1 calf was inoculated and given feed with added monensin at the dosage level of 10 g/906 kg of feed; and 2 calves were inoculated and given medicated feed with added monensin at the dosage level of 20 g/906 kg or 30 g/906 kg. In the 4 other pens (6 to 9), the calves were inoculated with E zuernii and otherwise were given feed without or with added monensin as in pens 2 through 5. Another group of 5 calves (all kept in 1 pen), served as noninoculated, nonmedicated controls. At 14 days after inoculations with E bovis, the single calves in each of the 4 pens that were given the nonmedicated feed began to show clinical signs of coccidiosis and discharged increasing numbers of oocysts. The other inoculated calves (given monensin) had fewer clinical signs and discharged fewer oocysts in the feces as the level of medication in the feed increased. The calves inoculated with E zuernii developed only moderately severe infections when compared with those inoculated with E bovis. Inoculated (with E bovis) nonmedicated calves had severe reductions in feed consumption and weight, and 3 of 4 died.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

14.
Faecal samples from 136 sheep from four different locations in South Australia were examined to determine the types and numbers of Eimeria spp. present. Coccidian oocysts were detected in 80% of the sheep and 11 different species of Eimeria were identified. The species detected (and their prevalence) were E. crandallis/E. weybridgensis (76%), E. ovina (55%), E. ovinoidalis (54%), E. granulosa (49%), E. parva/E. pallida (44%), E. intricata (37%), E. ahsata (31%), E. faurei (24%), and E. punctata (1%). No major differences were observed in the patterns of infection between the four locations examined. Faecal samples were also collected each month for a year from 48 lambs at two of the locations and oocyst counts were found to decrease markedly in all lambs after 6 months of age but to persist at low levels until the end of sampling at 17 months of age.  相似文献   

15.
Faecal Eimeria oocyst excretion and levels of antibodies to first generation merozoite antigen of E. bovis in sera and colostra were followed in 86 and 70 cow-calf pairs in northern (group EF) and central Germany (group H), respectively, over periods of 3 weeks before to 3 weeks after calving in cows and from birth to an age of 63 days in calves. Oocysts were found in 30 and 7.7% of cows in groups EF and H, respectively. They belonged to 10 (group EF) and four Eimeria spp. (group H) with E. bovis, E. ellipsoidalis, E. auburnensis and E. zuerni as the most frequently occurring species. Prevalence and intensity of oocyst excretion varied with time resulting in peak values around the date of parturition, particularly in the case of E. bovis. Peak values at the time of parturition were also seen in case of strongyle egg excretion. Seven (group H) and nine Eimeria spp. (group EF) were found in the calves. The predominant species E. ellipsoidalis, E. zuerni, E. bovis and E. auburnensis were detected for the first time earlier after birth (3-5 weeks) than the others. The prevalence of Eimeria infections increased to 67.1% (group EF) and 50.1% (group H) 9 weeks after birth. Specific IgM and IgA antibody levels (the latter only determined in group EF) in cow sera remained almost constant throughout the observation period, whereas IgG(1) and IgG(2) levels were reduced at the time of parturition. Levels of specific antibodies in sera and colostra were significantly correlated. Except IgM antibodies, significant inverse correlations were found in cows between intensity of infection with E. bovis and specific serum IgG (group H) and IgG(2) (group EF) antibodies. Antibodies to E. bovis were detected in calves sera only after colostrum intake with significant correlations between levels in calves sera and colostra. Levels decreased, starting within the first week of life (most conspicuously in case of IgM and IgA) until the third week. Subsequently, but except IgG(1) antibody concentrations increased until the end of the observation period. Interrelations between antibody levels and the total amount of E. bovis oocysts excreted by the calves until the ninth week of life varied with the age of the animals. Inverse relationships in the first 3 weeks of life as suggested by negative correlation coefficients could not be proven statistically. Thus, there is no unambiguous proof for immunoprotection of calves against E. bovis via maternal immunity. Considering antibody levels in the 3-9 weeks old calves significant direct correlations with E. bovis oocyst excretion were found in case of IgM, IgG(2) and IgA, reflecting an active immune response of young calves to coccidial infection.  相似文献   

16.
Prevalences and intensities of excretion of faecal stages of Giardia duodenalis, Cryptosporidium parvum, Eimeria spp., Strongyloides papillosus and strongyles were determined in a German upland area in German Angus (GA) and German Simmental (GS) suckler of beef cattle herds covering two winter housing periods and the grazing season between them. Influences of the housing systems applied (maintenance on deep litter with (DL+) and without run-out (DL--), on slatted floor (SF) or by winter run-out yarding (WO)), breed differences and genetic influences by the sire were determined by statistical analyses; levels of IgG antibodies to E. bovis antigen were measured by ELISA. G. duodenalis was observed with a maximum prevalence of 38% in 4 weeks old calves, a cumulative incidence of 58% 9 weeks after birth and with generally low intensities. C. parvum infections were relatively rare with cumulative incidences of 20--25% in week 5 after birth. Highest prevalences were associated with housing system DL-- and a long-lasting calving period. Cumulative incidence of Eimeria spp. was almost 100%. E. bovis predominated by far followed by E. ellipsoidalis/zuernii. Mean maximum intensity of 1000 OpG occurred in week 7 after birth. Up to an age of the calves of 7 weeks >75% of all oocysts belonged to E. bovis. Prevalences and excretion intensities were lowest under the housing conditions SF and WO. Maternal antibodies in calves to E. bovis antigen were directly and inversely correlated with mean OpG values in GA and GS calves, respectively. S. papillosus was common with a cumulative incidence of 53% 9 weeks after birth and occurred independent of the housing system. Mean strongyle egg prevalence was 50% with 50--100 EpG by means throughout the grazing season. Egg excretion intensity in the early months of grazings was correlated with the age of the calves at turnout to pasture. Under the conditions of housing system DL-- GA calves could better control S. papillosus infections than GS calves whereas the latter were generally more effective in controlling the protozoa. Results of ANCOVA suggest significant paternal influences on S. papillosus infections in both breeds and on the protozoal infections in GA calves. The former could be disclosed independent of the housing system whereas environmental factors played an important role in the latter cases.  相似文献   

17.
The faeces of 205 domestic cattle (Bos taurus) from 5 regions of Saudi Arabia were examined for the presence of coccidian parasites. The following species of Eimeria were recovered: Eimeria auburnensis, E. bovis, E. cylindrica, E. ellipsoidalis, E. subspherica, E. wyomingensis and E. zuerni. A total of 34.1% of the individual faecal samples were positive for the presence of coccidial oocysts. Mixed infections of 2-4 species were found in 15.7% of the specimens. E. zuerni and E. bovis occurred most frequently and were generally the most predominant species. The incidence of coccidia-infected cattle was higher in the eastern region.  相似文献   

18.
Sixty-one fecal samples were collected from adult alpacas and crias (ages 10 weeks to 10 years) on two farms in central Maryland. The farms raised both suri (silky-haired) and huacaya (crimpy-haired) breeds. Females and crias were housed together on pasture, whereas older/breeding males were maintained on separate pastures. Samples were subjected to a density gradient centrifugation protocol to concentrate parasites and remove fecal debris and were examined by immuno-fluorescent and differential interference contrast microscopy. Oocysts of Eimeria spp. were noted in 14 fecal samples, 6 on MD-1 and 8 on MD-2. Based on oocyst morphometrics two species of Eimeria were present: E. punoensis (19.2 microm x 16.5 microm) and E. alpacae (23.7 microm x 19.5 microm). Five animals shed exclusively E. punoensis, seven shed exclusively E. alpacae, and two had mixed infections. The Eimeria infections were not associated with obvious clinical signs. To determine the presence of Cryptosporidium and Giardia species and genotypes, DNA was extracted from feces and subjected to PCR utilizing specific primers for the ssu-rRNA gene for both parasites. All PCR positive samples were further analyzed by DNA sequencing to identify the species or genotypes that were present. Assemblage A, G. duodenalis was detected in fecal samples from two alpacas on MD-1 and in one alpaca on MD-2. Assemblage E, G. duodenalis and Cryptosporidium spp. were not detected on either farm. Although the prevalence on these two farms was low, alpacas can harbor zoonotic G. duodenalis, and this should be borne in mind by persons interacting with the animals.  相似文献   

19.
Coccidiosis, often caused by Eimeria zuernii infection, is an important diarrhoeal disease in calves [Fitzgerald, P.R., 1980. The economic impact of coccidiosis in domestic animals. Adv. Vet. Sci. Comp. Med. 24, 121-143]. Infection trials were performed to investigate the effects of experimental E. zuernii coccidiosis on clinical blood chemistry in calves. Three groups of calves were formed: group 1 (n=14) served as uninfected control group, group 2 (n=11) was infected with 150,000 sporulated E. zuernii oocysts per calf, and group 3 (n=16) was infected with 250,000 sporulated E. zuernii oocysts per calf. Measurements throughout the prepatent and the patent period revealed a marked influence of E. zuernii infection on the following parameters: total protein, albumin, urea, bilirubin, creatine kinase, free fatty acid concentration, and cholesterol. Aberrances in these were most pronounced in group 3. No significant and/or distinct changes after infection could be detected in blood glucose concentration. E. zuernii infection impairs intestinal function and induces catabolic metabolism in affected calves. Bilirubin, urea and cholesterol concentration, and creatine kinase activity were particularly affected indicating catabolism of protein and lipids.  相似文献   

20.
The prevalence of Cryptosporidium, Giardia and Eimeria, in healthy, asymptomatic, post-weaned and mature cattle was investigated on three Maryland farms. One farm, a dairy research facility, had 150 multiparous Holstein milking cows; 24 were examined and Cryptosporidium andersoni was detected in three (12.5%) but neither Giardia nor Eimeria was detected. The second farm, a commercial dairy, had 57 multiparous Holstein milking cows and an equal number of heifers. Of 19 cows examined, C. parvum, Giardia duodenalis, and Eimeria bovis and/or E. ellipsoidalis were detected in two (10.5%), two (10.5%) and one (5.26%) cow, respectively. Of 23 heifers examined, C. parvum, Giardia, and E. bovis and E. ellipsoidalis, was detected in two (8.7%), four (17.4%), and five (21.7%), heifers, respectively. The third farm, a beef cattle breeding and genetics research facility, had 180 7- to 9-month old purebred black Angus. Of 118 examined for C. parvum and Giardia, 34 (28.8%) and 44 (37.3%) were positive, respectively, of 97 examined for E. bovis and/or E. ellipsoidalis 32 (33.0%) were positive. These findings, based on a method with a minimum detection level of 100 oocysts of C. parvum/g of feces, which underestimates the number of infected cattle, clearly demonstrate the presence of low level, asymptomatic infections in post-weaned and adult cattle in the United States and indicate the potential role of such cattle as reservoirs of infectious parasites.  相似文献   

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