首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 171 毫秒
1.
Clinical mastitis in dairy cows is commonly treated with intramammary (IMM) antimicrobial agents. Pharmacokinetic data are used to design treatment regimens and determine withholding times. In some pharmacokinetic studies, investigators measure antimicrobial concentrations in foremilk, whereas in others, they use bucket milk or do not specify the milk fraction sampled. Our objective was to compare antimicrobial concentrations in foremilk, bucket milk, and strippings after IMM treatment of six healthy Holsteins. One mammary gland/cow was infused with 200 mg of cephapirin (CEPH) after each of the two milkings, using different milking frequencies and treatment intervals in a randomized crossover design. Treated glands were sampled at the first milking following each infusion. Antimicrobial concentrations in milk were measured using HPLC/MS/MS. CEPH concentration was higher in foremilk (geometric mean 44.2 μg/mL) than in bucket milk (15.7 μg/mL) or strippings (18.5 μg/mL), as it was true for desacetylcephapirin (DAC) (59.5, 23.0, and 30.2 μg/mL, respectively). This finding, which was based on milk samples collected at the first milking after IMM infusion, suggests that pharmacokinetic data based on drug concentrations in foremilk may be misleading. Strippings were more representative of bucket milk than foremilk. The relationship between milk fraction and antimicrobial concentration should be investigated for other IMM antimicrobial agents. Meanwhile, it is essential that pharmacokinetic and residue studies report the fraction of milk that was analyzed.  相似文献   

2.
Variation in milk composition and milk polymorphonuclear neutrophil leukocyte (PMN) characteristics and functions among quarter milk fractions were investigated in order to evaluate the optimal fraction for the determination of local immune response. Five fractions were classified during milking: foremilk (I), cisternal milk (II), main milk (III), strippings (IV) and residual milk (V). Somatic cell count (SCC), fat, protein, lactose, sodium, potassium, chloride, polymorphonuclear leukocyte necrosis, apoptosis and oxidative burst were analysed in each fraction. The logSCC and fat concentration were highest in residual milk (P < 0.05), whereas protein and lactose concentration were highest in the earliest fractions (I, II, III) (P < 0.05). Polymorphonuclear neutrophil leukocyte necrosis was lowest in strippings and residual milk (P < 0.05), and PMN apoptosis was lowest in residual milk (P < 0.001). The highest percentage of PMN with oxidative burst was found in residual milk (P < 0.05), and was associated with the highest mean fluorescence intensity (MFI) (P < 0.05). In conclusion, late fractions have more PMN and more active PMN. Nevertheless, it is suggested that each fraction is appropriate in the study of local immune response of the mammary gland, however the fraction used in the study has to be specified.  相似文献   

3.
Concentrations of progesterone (P4) were determined using enzyme immunoassay kits on plasma and milk samples obtained on the same days from 18 lactating dairy goats. Progesterone profiles documenting anestrus, short estrous cycles, normal estrous cycles, a prolonged follicular phase, and prolonged luteal phases were established. When plasma P4 concentrations were used as an accurate indication of the presence or absence of functional luteal tissue, milk P4 concentrations agreed with plasma determinations in 79.4% of the 465 samples tested. Milk samples could not be used to make a definitive decision because of marginal values in 11.2% of the determinations. Milk P4 concentrations were high when plasma P4 concentrations were low in 6.2% of the paired samples, especially those obtained around the time of estrus when peripheral P4 concentrations were changing rapidly. The remaining 3.2% of milk samples had low milk P4 concentrations when plasma P4 concentrations were high. Composite milk from 8 does in estrus or 8 does in the luteal phase was not consistently different from strippings in butterfat percentage or P4 concentration.  相似文献   

4.
The susceptibility of lactating bovine udder quarters fitted with a polyethylene intramammary device to infection was investigated. Following experimental challenge with Streptococcus agalactiae or Staphylococcus aureus, the incidence of infection was significantly (p less than 0.05) lower in intramammary device-fitted quarters compared to control quarters. In general, total foremilk and strippings milk somatic cell counts for intramammary device-fitted and control quarters were not significantly (p less than 0.05) different. Differential foremilk and strippings milk somatic cell counts were significantly (p less than 0.05) higher in samples from intramammary device-fitted quarters compared to control quarters.  相似文献   

5.
Data from Ontario dairy cattle herds which had had a high average milk gel index for 1978 (cases) and from other herds which had had a low average during the same period (controls) were collected and analyzed using case control techniques. The purpose of the study was to contrast factors of husbandry and management between the two groups and to determine the relative contribution of each of these factors on mastitis (as determined indirectly by the milk gel index) at the herd level.

Control herds had higher average production levels than did case herds, shipping 1807 litres more milk per cow per year. Milk from control herds averaged 0.06 percentage points higher in butterfat, 0.19 percentage points higher in lactose and 0.05 percentage points lower in total protein. However, many factors can influence production, therefore these latter differences, in both shipped milk and composition, can not be attributed solely to differences in the prevalence of mastitis between the two groups.

Control herds were more likely to use teat dip, receive regular veterinary service, use dry cow antibiotic preparations and have knowledge concerning subclinical mastitis than were case herds. Control herds also tended to raise more of their own replacements, have a higher culling rate for reasons of low production and have a more modernized dairy operation. Case herds, on the other hand, were more likely to scrutinize foremilk, use more milking units per operator and wait longer between the start of stimulation and attachment of the milking unit.

The study confirms, under natural field conditions, the importance of integrated mastitis control practices and also reaffirms the relative importance of practices such as the use of teat dips and dry cow antibiotic preparations.

  相似文献   

6.
The aim of this study was to investigate whether the skim milk progesterone concentrations at artificial insemination (AI) and day of rise of post-ovulatory progesterone concentration thereafter affect the conception and embryonic death rates in repeat-breeding cows. Milk samples were obtained from 96 repeat-breeding cows that failed to conceive to three or more AIs. The samples were taken from the cows at the day of AI and three times/week until day 45 post-AI. Skim milk was obtained after centrifugation and used for progesterone assay. The cows with a progesterone concentration more than 0.5 ng/ml at AI showed a significantly higher incidence of late embryonic death than those having a progesterone concentration<0.5 ng/ml at AI (p<0.01). As the progesterone level at insemination rose, conception rate declined. A negative correlation was shown between conception rate and skim milk progesterone level at AI. Of 56 cows showing a rise of progesterone to 1 ng/ml or more within 6 days after AI, 28 cows (50%) conceived. On the contrary, only eight of 39 cows (20.5%) conceived when the progesterone rose up to 1 ng/ml after day 6 post-AI. We concluded that increased progesterone concentration at the time of AI and delayed rise of progesterone post-AI might lead to decrease in fertility in repeat-breeding cows.  相似文献   

7.
No differences in blood samples were found out when the biochemical parameters in arterial and venous blood of dairy cows were compared before and after milking. Negative correlation coefficient (r = -0.3460) approaching the significance level was determined by comparing the values for milk yield on the day of sampling (in ascending phase of lactation) and protein content in venous blood after milking, and significant negative correlation coefficient (r = -0.3813) for daily milk yield and gamma-globulin concentration in venous blood before milking. The relationship between butterfat content on the day of milking and the values of alkaline phosphatase can be characterized by significant up to highly significant negative correlation coefficients in all three blood samples (r = -0.3232 to -0.3908).  相似文献   

8.
Individual quarter samples from some 19 cows on average were investigated monthly over 12 months for determining the udder health status of cows and the glucose concentrations of foremilk and strippings. Foremilk showed a mean 0,1311 mM concentration of glucose which remained fairly stable during the period of investigation and lactation. A fluctuating mean value of 0,2037 mM was determined in strippings in which glucose levels were consistently and appreciably higher than those of foremilk. Foremilk from completely normal quarters and others affected by non-specific cellular reaction, relevant or irrelevant teat canal infection and aseptic or septic subclinical mastitis, showed mean glucose concentrations of 0,1410; 0,1392; 0,1337; 0,1417; 0,1262 and 0,1248 mM, respectively. Strippings from the same quarters showed corresponding values of 0,2056; 0,2861; 0,2100; 0,1733; 0,1661 and 0,1617 mM glucose.  相似文献   

9.
In experiments on goats it was found that the binding of chlorpromazine (Cpz) to the proteins in plasma and milk ranged between 91–99 and 91–97 %, respectively, and was independent of the drug concentration in the samples. The in vitro binding of chlorpromazine in whole milk (96%) was significantly higher (P<0.01) than the protein binding in skim milk (91%) because the drug was concentrated in the butterfat. The concentration of Cpz was always higher in the milk than in the corresponding plasma samples. The renal clearance of Cpz in goats with normal urine pH was very small (0.16 ml min-1) due to the high degree of plasma protein binding and of back diffusion. The mechanisms involved during the renal excretion of Cpz in goats included glomerular filtration, probably active tubular secretion and pH dependent back diffusion.  相似文献   

10.
The purpose of this study was to determine the optimal interval between 2 consecutive milk progesterone samples for the detection of cyclicity in dairy cows. Two hundred and thirty-six postpartum periods were monitored with thrice-weekly whole milk progesterone assay. Cyclicity was determined from elevation of the progesterone level. Animals which had started to cycle by 50 or 60 days post partum were included in the study. The last of the 2 samples was taken at 50 or 60 days post partum, respectively.The lowest percentage of false diagnoses (9.0%) in cows which had started to cycle by 50 days post partum was obtained when the samples were taken at 8 days’ interval. In cows which had started to cycle by 60 days post partum the lowest percentage of false diagnoses (1.1%) was obtained when the samples were taken at 10 days’ interval.  相似文献   

11.
The Feulgen reaction is examined by absorption photometric measurements at 565 nm with correction for varying background absorption at 485 nm. This correction is done to improve the accuracy of measuring.Examinations of the accuracy of analysis for the Feulgen reaction and the direct microscopic cell counts show that the former is of the same order, when the cell count is made at a working factor of 17,000.The standard deviations expressed in percentage of the reaction value are greater the lower the reaction is, whereas the absolute standard deviation is reduced.It has been demonstrated that addition of a few (< 5) µg of DNA per ml milk can give Feulgen reaction. The coefficient of correlation between log added amount of DNA and log Feulgen reaction is 0.98.The coefficient of correlation between log Feulgen reaction and log cell content depends on the accuracy at which both determinations are made. In a determination of cell content at a working factor of 550, and several determinations of the Feulgen reaction the coefficient of correlation (r) is found to be 0.98. A cell content determination at a working factor of 20,000 and a single determination of Feulgen reaction on each milk sample yields r = 0.83.An examination of foremilk samples of the same cell content reveals on an average the highest reaction in mastitis-affected quarters which have been infected within the last 4 weeks. Quarters that have been infected for more than 4 weeks, on an average show the second highest reaction, whereas quarters of physiological cell number show the lowest reaction.The DNA-content in most cases is found to be too high compared with the number of cells counted microscopically. The DNA-content in centrifugated cells corresponds to the one calculated theoretically. The surplus DNA-content can be demonstrated in the cell-free skim milk fraction, probably originating from destroyed cells.The studies performed suggest that determination of the content of 2-deoxyribose in milk by means of the Feulgen reaction is a more correct measure of the cell content in the milk than is the microscopic cell count. Studies are being continued for illustration of these conditions.  相似文献   

12.
【目的】本研究基于试验测定的中国荷斯坦牛血液和乳汁中的孕酮浓度表型，探究了影响中国荷斯坦牛体内孕酮分泌的环境因素、孕酮浓度的变化趋势、孕酮浓度与乳成分的关联程度以及血乳中孕酮浓度的预测方法。【方法】试验于2021年8月在北京、山东两个牧场采集不同胎次、泌乳阶段和妊娠状态的中国荷斯坦泌乳牛的奶样、尾根血样，测定孕酮浓度，最终获得402条乳汁孕酮浓度和298条血液孕酮浓度表型用于数据分析。对孕酮浓度进行数据转化使其近似服从正态分布后，采用固定效应模型探究胎次、泌乳阶段、妊娠状态、牧场等固定效应对奶牛孕酮表型的影响，运用R语言cor函数计算孕酮与各乳成分间的关联，并利用偏最小二乘法和个体及乳成分信息对孕酮浓度进行预测，以建立孕酮浓度表型高通量获取手段。【结果】妊娠状态对转化乳汁孕酮浓度存在极显著影响（P<0.01），胎次、场效应对转化乳汁孕酮浓度均有显著影响（P<0.05），而转化血液孕酮浓度只受到妊娠状态影响（P<0.01）；全乳固体、乳脂率、乳蛋白率、脂蛋比与转化乳血孕酮浓度均存在显著或极显著的正相关关系（r=0.14~0.37，P<0.05；P<0.01）；基于本试验数据，乳成分与个体信息对转化乳血孕酮浓度的预测准确性不高（R2=0.030~0.17），但如果增加血液或乳汁的转化孕酮浓度对乳汁或血液的转化孕酮浓度进行预测，预测效果则有大幅提升（R2=0.40）。【结论】影响泌乳期中国荷斯坦牛转化孕酮浓度的因素除妊娠状态外，可能还包括饲养条件与胎次。此外转化孕酮浓度与乳脂率、乳蛋白率等乳成分呈极显著相关。基于乳成分信息与转化孕酮的关系，获得了对中国荷斯坦牛乳血转化孕酮浓度预测的可用策略，为今后牧场的繁殖辅助管理、奶牛育种新性状研发以及孕酮浓度的高通量获取等提供了新思路。  相似文献   

13.
A field study was conducted to determine the usefulness of milk progesterone determination at the time of breeding to confirm estrus and at 21 days postbreeding to detect open cows. Twenty-seven dairy farmers collaborated in this study by providing milk samples on the day of breeding and 21 days later, together with pregnancy diagnosis data and information on herd reproductive management. Herd size ranged from 15 to 175 cows, the average being 65 milking cows. Six hundred and sixty-seven breeding-day samples and 472, 21-day samples were provided by the farmers. Analysis of milk samples for progesterone by a solid phase radioimmunoassay showed that only 32 (4.8%) of the services were performed when the cow was not in estrus (progesterone > 1 ng/mL). Of the 472, 21-day samples, 337 (71%) showed progesterone levels of > 1 ng/mL, while 135 (29%) showed progesterone levels of < 1 ng/mL. Subsequently, 243 (72%) of the cows with progesterone > 1 ng/mL and eight (6%) of the cows with progesterone < 1 ng/mL were diagnosed pregnant by transrectal palpation, giving a pregnancy rate of 53%. Progesterone concentration on the day of breeding was not associated with season or herd size. However, progesterone concentration at 21 days and pregnancy rate were associated with herd size. These results indicate that fertillzation failure and/or early embryonic mortality, rather than inaccurate detection of estrus, are the major reproductive problems encountered by the dairy farmers in British Columbia. Furthermore, progesterone values at 21 days were closely related to reproductive status and indicate the usefulness of milk progesterone assay for the early detection of open cows.  相似文献   

14.
Antibacterial factors act as innate immune components, which respond as soon as bacteria enter a living organism. To prevent and treat mastitis in cattle, understanding the concentrations of these substances inside the udder is important; however, they remain to be studied. In this investigation, the concentration of lingual antimicrobial peptide (LAP), S100 protein (S100A7), lactoferrin (LF), and immunoglobulin antibody were measured in the different fractions of foremilk. Lactating Holstein cows were examined, and 10 foremilk fractions were obtained from sequential samples up to 150 ml. The LAP concentrations in milk samples increased until 25 ml. The LF concentrations increased up to the 10 ml fraction, then stabilized at low level after the 50 ml fraction. For S100A7, some fractions had significantly higher (p < .05) concentrations than the 5 or 10 ml fractions. The IgA antibody concentration increased up to the 5 ml fraction, then after 50 ml fraction showed relatively low concentrations. This investigation determined the concentration patterns of LAP, LF, S100A7, and IgA antibody secreted in milk inside the udders of healthy lactating cows as baseline data. These distinct concentration patterns might indicate various protective responses.  相似文献   

15.
Vaginal and milk temperatures were measured at each milking in 15 postpartum Friesian cows showing recurrent oestrous cycles for a total of 33 oestruses. A significant increase (P<0.001) in both vaginal and milk temperature of 0.29±0.05°C was shown between the milking prior to oestrus and the milking when oestrus occurred. Cows were divided into 3 groups: showing either a definite temperature rise (27% of cases), a limited temperature rise (60%) or no temperature change (13%) associated with oestrus. Various methods of analysis were investigated to determine the usefulness of the milk temperature data for oestrus prediction. The optimum compromise of high oestrus detection rate (73.3±16%) and lowest percentage of false positives (10.8±3.0%) was based on a temperature rise on the day of oestrus of 0.2°C over the corresponding mean temperature of the preceding three days. These rates suggest the measurement of milk temperature is less useful for oestrus prediction than some earlier reports have indicated.  相似文献   

16.
Intramammary devices (IMD) were abraded with medium-grade emery cloth or were left smooth. One IMD of each type was inserted into a mammary quarter of each of 5 lactating cows. The remaining 2 quarters served as controls. Quarter foremilk, bucket milk, and stripping milk samples were collected for 3 consecutive days at 2 weeks after IMD insertion, and milk somatic cell counts (SCC) were determined. Milk samples also were collected immediately after and 0.5, 1, 2, 4, 6, 8, and 11 hours after milking. All quarters were challenge exposed with 250 colony-forming units of Streptococcus uberis at 2 months after IMD insertion. Foremilk and stripping milk samples were collected for bacteriologic culture and SCC at the next 10 milkings. Mean foremilk, bucket milk, and stripping milk SCC (X 10(6) cells/ml) were 0.18, 0.07, and 0.91, respectively, for quarters with abraded IMD; 0.06, 0.05, and 0.43, respectively, for quarters with smooth IMD; and 0.03, 0.03, and 0.15, respectively, for control quarters. Mean SCC after milking (X 10(6) cells/ml) for the various intervals were 0.70, 1.29, 0.70, 0.97, 1.15, 1.17, 0.77, and 0.85 for quarters with abraded IMD; 0.43, 0.62, 0.61, 0.45, 0.64, 0.60, 0.31, and 0.26 for quarters with smooth IMD; and 0.15, 0.24, 0.15, 0.19, 0.15, 0.15, 0.14 and 0.06 for control quarters. After challenge exposure, 2 of 5 of the quarters with abraded IMD, 4 of 5 of the quarters with smooth IMD, and 8 of 9 control quarters became infected. Results indicated that abraded IMD increased SCC in stripping milk to concentrations that provided 60% protection against challenge exposure with S uberis.  相似文献   

17.
"增奶威"饲喂奶牛的效果试验   总被引：2，自引：0，他引：2  
选择中国荷斯坦泌乳牛饲喂增奶威活性饲料添加剂。结果表明:增奶威可提高产奶量5-5.8%,增加乳脂率0.04%,牛奶中的体细胞数下降57%,胎衣滞留比率降低,投入产出比达到1:3.8。  相似文献   

18.
The content of urea in milk was studied as a parameter of dairy cow nutrition. It is possible to use for the analysis whole milk where the values are lower by 5.96% than in defatted milk (this fact must be borne in mind when the results are interpreted). Protein can be removed from whole milk within 24 hours after sampling provided that it was placed in a refrigerator within four hours from milking to be stored there at 4 degrees C. In such a case the decrease in urea level is not greater than 4%. No significant differences were found between urea concentration in milk collected by stripping and that in bulk milk. It is not recommended to take samples at the end of milking because such milk contains less urea. Urea concentration in the bulk milk samples corresponds to the average urea concentration in the milk samples taken from dairy cows in the stable.  相似文献   

19.
体型外貌性状是奶牛重要的选种指标,是奶牛保持高产、稳产、健康长寿的关键所在。本研究的目的在于探寻上海地区荷斯坦牛体型外貌与产奶性状的相互关系。、随机选取上海市28个奶牛场总计871头第二泌乳期荷斯坦母牛作为研究对象,记录并测定其305d产奶量、乳脂率、乳蛋白率3个产奶性状,体型外貌性状采用9分制评分结果．利用相关分析方法确定体型总分及7个体型外貌性状与各产奶性状之间的关系,结果表明,体型总分与305d产奶量、乳脂率呈极显著正相关（P〈O．01）;体躯容量性状和产奶量呈极显著正相关：肢蹄性状与乳脂率、乳蛋白率呈极显著正相关,与产奶量显著正相关（P〈O．05）;尻部性状与乳脂率呈显著正相关．。最终得出肢蹄、体躯容量、尻部性状是影响荷斯坦牛产奶性状的最主要因素,体型外貌鉴定评分对于筛选高产、优质荷斯坦母牛及选种选配工作具有指导意义。  相似文献   

20.
The skim milk progesterone profile was assessed by radioimmunoassay, without extraction, from the day of insemination (day 0) until the cows were dried off on day 225 of gestation. A total of 418 samples were collected from 154 pregnant Holstein cows. The daily variation in skim milk progesterone was recorded from day 1 until day 45 of pregnancy to detect the commencement of progesterone secretion from the corpus luteum after insemination. Subsequent determinations were made every 2 weeks from day 46 until lactation ceased. On the day of artificial insemination and for the first 2 days after insemination, all the cows had a basal progesterone concentration <0.1 ng/ml. A rise in progesterone (0.2±0.1 ng/ml) was first detected on the third day after insemination. The progesterone values then increased significantly (p<0.001) until day 15.The values then remained nearly constant (2.5–3.5 ng/ml) until day 106 of pregnancy, when they began to decline. Between days 120 and 180 of gestation, progesterone was significantly decreased (2.2–2.9 ng/ml) before it rose again to the previous plateau (3.5–3.9 ng/ml) around day 180. The progesterone concentration then remained at the higher level until the animals were dried off.Abbreviations AI artificial insemination - RIA radioimmunoassay  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号