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1.
开菲尔粒菌相构成的研究   总被引:1,自引:0,他引:1  
开菲尔乳的风味和特性与开菲尔粒的菌相组成密切相关,对来自波兰的开菲尔粒中的微生物菌相进行分离鉴定表明,该开菲尔粒主要由干酪乳杆菌(9株)、乳酸乳球菌乳酸亚种(6株)、肠膜明串珠菌右旋匍聚糖亚种(3株)、酿酒酵母(2株)以及巨大克鲁维氏酵母(5株)构成.  相似文献   

2.
开菲尔是一种由开菲尔粒发酵的功能性酒精性的乳制品.在开菲尔粒发酵培养期间,对外加营养元素进行单因素与正交试验,以研究其对开菲尔多糖产量的影响.结果表明:在无菌灭菌乳中添加3%麦芽糖、4%蔗糖、4%葡萄糖时,开菲尔多糖的产量最高,达到1.92 g/L;在无菌灭菌乳中添加酵母膏0.2%、蛋白胨1%、L-酪氨酸0.004%时,开菲尔多糖的产量最高,达到2.07 g/L;在无菌灭菌乳中添加0.4% KH2PO4、0.08% MgSO4·7H2O、0.02%CaCO3、0.25%NaC1时,开菲尔多糖的产量最高,达到2.01 g/L.  相似文献   

3.
开菲尔粒中微生物菌群及分离鉴定研究进展   总被引:1,自引:0,他引:1  
范佳  李艳 《中国奶牛》2014,(14):40-43
开菲尔粒是发酵开菲尔的传统发酵剂,是由乳酸菌与酵母菌等众多微生物之间的共生作用而形成的粒状结构。因为其微生物菌群组成复杂且分布不均,一直是众多学者的研究重点。本文主要介绍了开菲尔粒中的微生物菌群及其之间的相互关系,并简介了各个菌种分离鉴定方法的研究进展。  相似文献   

4.
开菲尔粒是一个复杂的微生物共生体系,包含很多有益生作用的微生物,其主要功能菌的分离鉴定,对进一步制备复合发酵剂具有重要的意义。因此本文研究了一种开菲尔粒的主要组成菌。通过形态学特征、生理生化分析,初步分离纯化得出:该开菲尔粒样品主要由两株酵母菌、三株乳酸菌以及两株醋酸菌构成。经16SrDNA序列分析进一步确定其种属,得出其分别为Kluyverom ycesm arxianus和Pichiakudriavzevii、Lactobacilluspontis和Lactobacilluskefiri、Acetobacterlovaniensis和Acetobactercibinongensis。  相似文献   

5.
正在世界上很多地方,癌症是主要的健康问题。传统的癌症治疗方法不仅使患者痛苦、费用较高,而且不安全,因此,患者对通过功能食品或营养补充品治疗癌症的需求越来越大。开菲尔是一种传统发酵乳制品,具有显著的抗突变、抗癌特性。约旦研究人员分析发酵条件对开菲尔抗癌特性的影响。通过四唑盐比色法评价标准条件下制备的开菲尔提取物对7株癌细胞的抗癌活性,结果表明,结肠癌细胞和慢性粒细胞性  相似文献   

6.
通过多菌种混合发酵来制造与传统开菲尔饮品类似的含醇酸奶。对发酵条件优化的结果表明,在添加了50g糖和30g功能性蛋白粉的共800g牛奶基料中加入9个单位菌种,在34℃条件下发酵培养17h,所得的产品中酒精度最高,有一定的刹口清新感。  相似文献   

7.
从葡萄、提子、开菲尔粒中分离纯化出5株酵母菌,分别涂布于黄油表面,22℃培养60 d,每隔15 d对样品进行感观评定、酸值测定,60 d后分析各样品的过氧化值、脂肪酸组成,得到黄油的酸败与低碳链脂肪酸有关,两株酵母菌对黄油的酸败有抑制作用,延缓黄油释放出哈败味道.  相似文献   

8.
<正>波兰研究亚麻荠(Camelina sativa,CS),富含不饱和脂肪酸,在反刍动物中可以提高饮食的能量价值,也可增加乳中不饱和脂肪酸的含量(拟发表于2014年7月Small Ruminant Research)。设计基本饮食(对照组)和基本饮食加12g/100gCS浓缩干物质,测定羊乳的脂肪酸组成和羊乳制成的开菲尔。实验特别强调单烯和共轭亚油酸的同分  相似文献   

9.
用开菲尔粒为菌种发酵牛奶,经二次配料,筛选了最佳工艺,制成一种集营养、功能为一体的活性乳饮料。结果表明,以50%酸奶基料,42.7%纯净水,7%白砂糖,0.3%PGA,按照本文所制定的工艺,生产出的开菲尔饮品稳定性好、口感滑爽、风味优良。  相似文献   

10.
该研究揭示了类开菲尔粒发酵乳酸度受发酵条件影响的主次顺序分别为时间、温度、接种量;乙酸、乳酸含量受发酵条件影响的主次顺序分别为温度、时间、接种量;甲酸含量受发酵条件影响的主次顺序分别为温度、接种量、时间。1:10接种、37℃条件下发酵16小时产酸量较高.  相似文献   

11.
Functional foods including kefir have increasingly become as popular as before in the developing world. The health benefits of kefir including the antioxidative effects are still under evaluation. Vitamin E is also a well-known antioxidant. The biologically damaging effects of reactive oxygen species are controlled in vivo by a wide spectrum of antioxidant defence mechanisms. Dietary constituents of antioxidative vitamins and other nutrients may play an important role in protecting the body against oxidative damage. The study was carried out to investigate the protective effect of kefir against oxidative damage of CCl4 in mice, compared with the well-known antioxidant vitamin E. Three-week-old Swiss Albino mice, weighing 22-26 g were used for the experiment. At the end of the microbiological analysis of kefir, the averages of the total mesophilic aerobic colony counts, lactic acid bacteria, lactic streptococci, enterococci, and yeasts were found to be 1.04 x 10(9), 9.87 x 10(8), 4.38 x 10(8), 7.80 x 10(4) and 1.26 x 10(5) CFU/ml, respectively. While both vitamin E and kefir were found to have a protective effect against CCl4-induced damage, kefir was more protective. This may probably be the first study to compare the antioxidative action of kefir and vitamin E in the animal model.  相似文献   

12.
Functional foods including kefir have increasingly become as popular as before in the developing world. The health benefits of kefir including the antioxidative effects are still under evaluation. Vitamin E is also a well‐known antioxidant. The biologically damaging effects of reactive oxygen species are controlled in vivo by a wide spectrum of antioxidant defence mechanisms. Dietary constituents of antioxidative vitamins and other nutrients may play an important role in protecting the body against oxidative damage. The study was carried out to investigate the protective effect of kefir against oxidative damage of CCl4 in mice, compared with the well‐known antioxidant vitamin E. Three‐week‐old Swiss Albino mice, weighing 22–26 g were used for the experiment. At the end of the microbiological analysis of kefir, the averages of the total mesophilic aerobic colony counts, lactic acid bacteria, lactic streptococci, enterococci, and yeasts were found to be 1.04 × 109, 9.87 × 108, 4.38 × 108, 7.80 × 104 and 1.26 × 105 CFU/ml, respectively. While both vitamin E and kefir were found to have a protective effect aganist CCl4‐induced damage, kefir was more protective. This may probably be the first study to compare the antioxidative action of kefir and vitamin E in the animal model.  相似文献   

13.
为研究藏灵菇功能特性,以滴定酸度和感官评价为指标,通过单因素试验对藏灵菇脱脂发酵乳工艺条件进行初步优化,并通过体外实验对藏灵菇脱脂发酵乳抗氧化能力和降胆固醇能力进行测定。结果表明:确定藏灵菇接种量6%、发酵温度37 ℃、发酵时间7.5 h为藏灵菇脱脂发酵乳的最优工艺条件,此条件下制得的脱脂发酵乳酸度适宜、口感细腻、质地均匀,具有独特风味,乳酸菌数为1.48×108 CFU/mL,硬度为178.43 g,高于传统发酵乳,黏附性为-103.52 g·s,且非脂乳固体含量、蛋白质含量和滴定酸度均符合发酵乳标准GB 19302—2010《食品安全国家标准 发酵乳》,品质较好;藏灵菇发酵乳抗氧化能力虽与传统发酵乳差异不显著,但具有较好的降胆固醇能力,藏灵菇脱脂发酵乳胆固醇去除率高达37.98%,藏灵菇全脂发酵乳胆固醇去除率高达40.68%。  相似文献   

14.
In this study we investigated the effect of kefir on the levels of glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO) in the liver, stomach, spleen and colon of mice with colonic aberrant crypts formed by azoxymethane (AOM). Thirty 12 weeks old Swiss Albino mice averaging 31.5 g weight were used as experimental animals. The mice were separated into 3 groups. The first group was the control group, second group was the AOM and third group was the AOM+kefir group. We applied AOM to the second and third groups. Mice were fed ad libitum by laboratory rodent chow during the experiment period. Water was given to the first and second groups and third group received only kefir diluted with water (50%). AOM was injected subcutaneously to the second and third groups for 7 weeks (two times a week, 5 mg/kg). Six weeks after the final AOM treatment the animals were sacrificed and liver, stomach, spleen and colon samples were collected from all the groups. MDA level demonstrated an increase only in stomach for the third group (p < 0.001), while an elevation was observed for all of the four organs for the second group (spleen p < 0.001, liver p < 0.001, colon p < 0.01). GSH level showed an increase in the second group at stomach (p < 0.01) and colon (p < 0.001), while in the third group, a small increase was determined only at the colon (p < 0.05). NO level increased at all of the organs in the second group (spleen, liver, colon p < 0.001, stomach p < 0.05), but only at liver and colon in the third group 3 (p < 0.001). In conclusion these results showed that kefir plays an antioxidant role.  相似文献   

15.
The aim of this study was to investigate effects of kefir, a traditional source of probiotic, on coccidial oocysts excretion and on the performance of dairy goat kids following weaning. Twin kids were randomly allocated to one of two groups at weaning. Kids of the first group received 20 ml of kefir daily for 6 weeks (KEF), while kids in the control group were given a placebo (CON). Individual faecal samples were regularly (n = 18 per kid) taken to quantify the number of coccidial oocysts per gram of faeces (OpG). There were no differences between the groups in terms of body weight development (P > 0.05) and feed consumption. Kids of both groups were not able to consume enough feed to meet their nutrient requirements during the first 3 weeks following weaning. KEF had a lower frequency of OpG positive samples than CON (P = 0.043). Kefir did not affect the maximum oocyst excretion and age of the kids at the highest oocyst excretion (P > 0.05). KEF shed numerically 35% lower coccidial oocysts than the controls, which corresponded to a statistical tendency (P = 0.074) in lowering Log-OpG in comparison to CON. While KEF had a lower frequency of OpG positive samples and tended to shed lower OPG by around one-third, the frequency of diarrhea, level of highest oocyst excretion, and performance of the kids remained unaffected. Therefore, it is concluded that overall effects of kefir do not have a significant impact on sub-clinical infection and performance in weaned kids under relatively high-hygienic farming conditions.  相似文献   

16.
作为一种古老而又新型的发酵奶制品,Kefir的营养特性及其保健功能,愈来愈受到人们的喜爱。本文介绍了Kefir及其发酵剂开菲尔粒,并对Kefir的营养特性、功能特性及其相关制品的研究进展做一综述,对Kefir的研究进行了展望。  相似文献   

17.
Rifaximin is a kind of new drug for the treatment of dairy cow disease which antimicrobial spectrum is wide.It has a strong antibacterial activity and unique antibacterial mechanism.Rifaximin is suit for local administration because it is no mutagenic,abnormal toxicity,carcinogenicity and reproduction toxicity and so on.In this paper,the physical and chemical properties,action mechanism,pharmacokinetic,pharmacology and toxicology properties,clinical application were investigated and reviewed,so as to provide a theoretical basis for the application in prevention and treatment of animal diseases,and make veterinary workers have a comprehensive understanding of rifaximin and guide the clinical application.  相似文献   

18.
利福昔明是一种治疗奶牛疾病的新药,抗菌谱广,抗菌活性强,抗菌机制独特,无致突变、致畸、致癌及生殖毒性等,适合作为局部用药。作者综述了利福昔明的研究现状,并对其理化性质、作用机制、药动学、药理毒理学性质及在临床疾病防治中的应用进行了描述,为利福昔明在动物临床疾病防治应用方面提供理论依据,以便兽医工作者全面了解该药,进而指导其临床应用。  相似文献   

19.
1. This study was conducted to evaluate the effects of dietary β-glucan and kefir (a fermented milk product) on growth performance, blood profiles, relative organ weight and meat quality in broilers.

2. A total of 375 day-of-hatch mixed sex ROSS 308 broilers (BW of 46 ± 0.1 g) were used in a 5-week experiment and randomly allotted to one of the following dietary treatments: (1) NC, basal diet; (2) PC, basal diet + 40 mg/kg of avilamycin; (3) B, NC + 0.1% β-glucan; (4) K, NC + 0.1% kefir; (5) BK, NC + 0.1% β-glucan + 0.1% kefir.

3. During weeks 0–3, broilers in B, K and BK treatments had higher body weight gain (BWG) than those in NC treatment. During weeks 4–5, BK treatment had a higher BWG than NC treatment. Overall, broilers given PC, K and BK diets had higher BWG than those given NC diet. The feed efficiency ratio (FCR) was improved by PC treatment.

4. Relative liver weight was increased by B treatment, whereas the relative weight of breast meat and gizzard was higher in BK group than that in NC group. Broilers given PC, B and BK diets had greater breast meat redness value and reduced drip loss at d 5 and d 7. The cooking loss was also reduced by B and BK treatments compared with NC treatment.

5. In conclusion, the results suggested that inclusion of 0.1% β-glucan and 0.1% kefir, either individually or combined, would improve growth performance and benefit meat quality in broiler chickens.  相似文献   


20.
Alpha-2 adrenergic agonists have some unique properties that could theoretically make them useful in the perianesthetic period: they reduce the MAC of inhaled anesthetics, and they are reversible. They also have properties that may limit their usefulness, such as marked decreases in cardiac output. Their clinical utility awaits further studies.  相似文献   

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