共查询到19条相似文献，搜索用时 234 毫秒
1.
铜中毒对雏鸭某些血液指标的影响 总被引：6，自引：4，他引：6
选用1日龄天府肉鸭210只,随机分为3组,分别喂以对照日粮(Cu12.16mg/kg)、铜中毒日粮(Cu850mg/kg)和铜中毒日粮(Cu1050mg/kg)。试验期7周。以血液病理学方法观察了铜中毒对雏鸭某些血液学指标的影响。结果显示铜中毒组、组雏鸭分别于2周龄和3周龄开始出现临床症状,死亡18只和5只。血液病理学变化表现为,红细胞大小不等、变形变性、坏死溶解;红细胞数量和血红蛋白含量显著降低(P<0.01),血清谷丙转氨酶、谷草转氨酶活性显著升高(P<0.05或P<0.01),血清铜蓝蛋白活性下降。结果表明,铜中毒对雏鸭的生长发育和上述血液指标影响明显。 相似文献
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1日龄天府肉鸭健雏210只随机分为3组,日粮铜含量(mg／kg)分别为12.16(对照组)、580(Ⅰ组)和1050(Ⅱ组)观察七周。实验观察铜含量对雏鸭红细胞免疫功能影响的动态变化。结果,两观察组雏鸭红细胞C3bRR花环率和ICR花环率降低,红细胞免疫功能受损,1~7周龄与对照组比较差异极显著(P<0.01),ICR花环率1~7周龄与对照组比较除2周龄外差异显红细胞变形、变性,大小不等。结果发现C3bRR花环率和ICR花环率的降低与过量铜中毒引起红细胞的病理损害密切相关。 相似文献
3.
铜中毒对雏鸡血液生化指标影响的研究 总被引：5，自引：0，他引：5
1日龄艾维茵肉鸡健雏180只,随机分为3组,分别喂以对照日粮（Cu 11.92 mg/kg）、铜中毒Ⅰ日粮（Cu650 mg/kg）和铜中毒Ⅱ日粮（Cu 850 mg/kg）6周,以血液病理学方法观察铜中毒对雏鸡血液生化指标的影响.铜中毒Ⅱ组、铜中毒Ⅰ组雏鸡分别于2周龄和3周龄开始出现临床症状,分别死亡7只和3只.血液生化指标变化表现为红细胞胞体和胞核变形;红细胞数量和血红蛋白含量显著降低（P＜0.05或P＜0.01）,血清谷草转氨酶、乳酸脱氢酶活性和血清铜含量显著升高（P＜0.05或P＜0.01）,血清铜蓝蛋白活性显著下降（P＜0.01）.结果表明,铜中毒对雏鸡的生长发育和上述血液生化指标影响明显. 相似文献
4.
铜中毒对雏鸡红细胞免疫功能的影响 总被引：3，自引：0，他引：3
本文旨在研究铜中毒对雏鸡红细胞免疫功能的影响。选用1日龄艾维茵肉鸡健雏180只,随机分为3组,分别喂以对照日粮(含Cu 11.97 mg/kg)和铜中毒日粮(铜中毒I组,含Cu 650 mg/kg;铜中毒II组,含Cu850 mg/kg)6周。结果表明:两个铜中毒组雏鸡的RBC-C3bRR花环率和RBC-ICR花环率降低,红细胞免疫功能受损,肉鸡1~6周龄时与对照组比较差异显著(P<0.05或P<0.01),并与红细胞的形态改变相一致。RBC-C3bRR花环率和RBC-ICR花环率的降低与铜中毒引起的红细胞病理损害密切相关。 相似文献
5.
为探讨不同剂量甘氨酸铜替代高硫酸铜(以铜计250 mg/kg饲料)对断奶仔猪生长性能、血液中相关元素含量及理化指标的影响,试验选择96头平均体质量约(8.36±0.14)kg的断奶仔猪,随机分为4组,每组4个重复,每重复6头猪。对照组饲喂基础日粮+250 mg/kg硫酸铜(以铜计);试验1、2和3组在基础日粮中分别添加250、125和62.5 mg/kg甘氨酸铜(以铜计);试验期30 d。结果表明:与对照组相比,各试验组仔猪末质量差异不显著,试验3组日采食量下降了21.3%,料肉比改善了11.9%;血清铜含量试验1、2和3组分别比对照组提高10.43%、22.64%和19.53%;血清锌含量试验1和2组分别下降10.64%和2.67%,试验3组提高25.75%;血清铁含量试验2和3组分别提高34.8%和40.31%;试验1和2组铜蓝蛋白含量分别下降16.75%和21.25%,试验3组提高了17.44%;谷胱甘肽过氧化物酶试验1、2和3组分别下降2.1%、7.42%和8.83%;铜锌超氧化物歧化酶试验1和2组分别提高1.07%和9.47%,试验3组下降0.9%;谷丙转氨酶试验1、2和3组分别提高26.04%、13.87%和41.01%;谷草转氨酶试验1、2和3组分别提高11.43%、15.55%和22.43%。综合考虑,甘氨酸铜的适宜添加量为62.5 mg/kg。 相似文献
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研究日粮铜对育肥猪组织铜沉积和粪铜代谢率的影响,本试验选择来源相同且体重相近(30±1.05)kg健康状况较好的杜长大三元杂交生长猪共24头,随机分为4组,每组3个重复,每个重复2头猪。4个处理组分别饲喂4种不同硫酸铜水平的试验日粮,将最低剂量组的铜添加量设定为10 mg/kg(对照组),试验组分别添加铜含量为45 mg/kg(1组),135 mg/kg(2组),225 mg/kg(3组);添加后测定饲料中铜的实际含量分别为19.09 mg/kg(对照组),53.60 mg/kg(1组),154.00 mg/kg(2组),250 mg/kg(3组)。结果表明:在试验期间,胃、脾、胰脏和淋巴结组.织中铜含量在各个铜饲喂剂量下都处于较低水平,各组饲喂剂量之间差异不显著(P0.05);各处理组间心、肝、肺脏铜含量差异显著(P0.05);胫骨,肋骨、股骨骨质和股骨骨髓的积铜含量差异显著(P0.05);10 mg/kg(对照组)和45 mg/kg(1组)铜的消化率明显高于135 mg/kg(2组)和225 mg/kg(3组)(P0.05),但2,3组间铜的消化率差异极显著(P0.01)。结果表明,日粮中添加45 mg/kg的硫酸铜猪体内组织铜沉积率小,粪铜消化率高。 相似文献
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选用120头体重约35kg三元杂(长白×约克×杜洛克)生长育肥猪进行试验,研究在饲粮中添加高剂量铜对猪组织的影响。将试验猪随机分为5个处理,每个处理3个重复栏,每个重复10头。各处理添加的铜的剂量分别为:对照组8.0mg/kg、处理1组150.0mg/kg、处理2组225.0mg/kg、处理3组300.0mg/kg、处理4组375.0mg/kg。研究结果表明:(1)处理1、2、3、4组猪肝脏显著大于对照组(P<0.05);处理4组也显著大于处理1、2组(P<0.05)。(2)处理2、3、4组猪肝脏、肌肉组织铜含量显著高于对照组(P<0.05);处理4组也显著高于处理1、2、3处理组(P<0.05)。(3)处理1、2、3、4组猪肝脏、肌肉组织细胞线粒体的正常结构受到损坏,有的线粒体内已看不到线粒体嵴。 相似文献
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9.
文章旨在探讨日粮单独或联合补充微量元素铜和硒对肉牛生长性能、肌肉脂肪酸组成及血清抗氧化的影响。试验将平均体重为(389.98±1.67)kg的80只肉牛随机分为4组,每组4个重复,每个重复5头。对照组饲喂基础日粮,T1组在基础日粮中以亚硒酸钠形式添加1.5mg/kg硒,T2组在基础日粮中以硫酸铜的形式添加25mg/kg铜,T3组在基础日粮中以亚硒酸钠形式添加1.5mg/kg硒和以硫酸铜的形式添加25mg/kg铜。饲养试验共进行84d。结果:1.5mg/kg硒组肉牛末重和采食量较对照组分别显著提高了7.36%和38.69%(P<0.05),但料重比较对照组显著降低了17.02%(P<0.05),同时处理组肉牛平均日采食量较对照组分别显著提高15.06%、16.86%和20.08%(P<0.05)。对照组肌肉亚麻油酸含量较铜硒混合组显著提高62.50%(P<0.05)。对照组肉牛血清胆固醇含量也显著高于T1组(P<0.05)。对照组和T3组肌肉胆固醇含量显著高于T1和T2组(P<0.05)。与对照组相比,T2和T3组血清谷胱甘肽过氧化物酶活性分别显著降低46.42%和62.84%(P<0.05)。T1组血清超氧化物歧化酶活性较对照组和T2组分别显著提高10.64%和15.94%(P<0.05),同时对照组血清丙二醛含量较T1和T3组分别显著提高91.45%和73.90%(P<0.05)。结论:日粮补充铜或硒可以改变肉牛脂质代谢,补充硒可以改善肉牛饲料效率、血清抗氧化性能,降低血清和肌肉胆固醇含量。 相似文献
10.
日粮铜和维生素A及其互作效应对肉仔鸡生长性能和血液生理指标的影响 总被引：2，自引：2，他引：0
本试验选用1日龄艾维茵肉仔鸡448只,随机分为8组,每组4个重复,每个重复14只鸡,采用4×2(Cu×VA)完全随机设计,研究了日粮添加不同水平的铜(0、8、150、225 mg/kg)和维生素A(1 500、5 000 IU/kg)对肉仔鸡不同生长阶段(前期0～4周龄和后期5～7周龄)的生长性能及血液生理指标的影响.结果表明:高铜(150、225 mg/kg)显著降低前期体增重和采食量、血红蛋白生成和血沉(P<0.05);高铜有利于白细胞生成 (P<0.05);0 mg/kg铜组显著提高血红蛋白含量、后期红细胞压积和前期血沉(P<0.05);铜的不同添加水平对全期红细胞数目影响不显著(P>0.05).5 000 IU/kg维生素A组获得较好的生长性能、血红蛋白含量、红细胞数目,并显著提高前期红细胞压积和白细胞数目,但显著降低后期红细胞压积(P<0.05).铜和维生素A互作效应对前期体增重及全期料重比影响均显著(P<0.05),对前后期血红蛋白含量、血沉、白细胞计数及后期红细胞压积影响均极显著(P<0.01),且二者间存在互补作用,Cu(8 mg/kg)×VA(5 000 IU/kg)组与Cu(0 mg/kg)×VA(5 000 IU/kg)组对前期肉仔鸡生长性能、红细胞计数、血红蛋白含量、红细胞压积、血沉均较好.铜和维生素A互作效应对全期红细胞数目影响不显著(P>0.05).结果提示,在基础日粮铜水平为16～23 mg/kg时,铜的适宜添加量前期为8 mg/kg,后期为0～8 mg/kg;全期维生素A的添加量为5 000 IU/kg. 相似文献
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为探索高铜对雏鸭胸腺形态的影响,选用1日龄天府肉鸭360只,随机分为6组,分别喂以对照日粮（Cu8mg／kg）和高铜日粮（Cu100mg／kg,高铜Ⅰ组;Cu200mg／kg,高铜Ⅱ组;Cu400mg／kg,高铜Ⅲ组;Cu600mg／kg,高铜Ⅳ组;Cu800mg／kg,高铜V组）6周。结果表明：与对照组比较,高铜Ⅲ组... 相似文献
12.
实验性雏鸭锌中毒症的病理学研究 总被引：7，自引：0，他引：7
1日龄天府肉鸭健雏100只随机分为2组，分别喂以止常对照(Zn100mg／kg日粮)和锌中毒(Zn1300mg／kg日粮)日粮4周，进行系统的病理学研究。中毒鸭13d出现症状，18只死亡。尸检主要见腿部肌肉色白或灰白色，外观似蜡样；肌胃平滑肌色淡灰白；消化道充满黑褐色煤焦油样内容物。光镜下，骨骼肌纤维肿胀变性坏死；心肌和肌胃平滑肌局灶性坏死；胸腺、腔上囊、脾脏淋巴细胞减少。电镜观察，免疫器官淋巴细胞线粒体肿胀、嵴断裂。血液病理学变化主要是碱性磷酸酶活性、血清总蛋白与球蛋白含量以及红细胞总数和血红蛋白含量降低，血清谷丙转氨酶活性和锌含量升高。结果表明，高锌对鸭可产生明显的毒害作用。文中还对发病机理进行了探讨。 相似文献
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180只1日龄艾维茵肉鸡健雏随机分为3组，分别饲喂对照（每千克日粮含铜11．97mg）和铜中毒（每千克日粮舍铜650mg，铜中毒Ⅰ组；每千克日粮含铜850mg，铜中毒Ⅱ组）日粮6周，以流式细胞术和酸性-α-醋酸萘酯酶（ANAE）染色的方法观测外周血T-淋巴细胞的动态变化。2个铜中毒组1～6周龄外周血T-淋巴细胞的ANAE阳性率均显著低于对照组（P〈0．05或P〈0．01），同时2个铜中毒组间比较也呈显著差异（P〈0．05或P〈0．01）。与对照组比较，2个铜中毒组2～6周龄CD4^＋T淋巴细胞数量均显著降低（P〈0．05或P〈0．01），CD8^＋T淋巴细胞数量变化不明显（P〉0．05），CD4^＋／CD8^＋比值降低（P〈0．05）。结果表明，铜中毒可抑制T-淋巴细胞的生成，降低其在外周血中的数量。 相似文献
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选用1日龄健康AA肉鸡120只随机均分成4组,使用硫酸铜作为铜源,饲喂玉米-豆粕型基础日粮,对照组饲料铜含量为11mg/kg,3个试验高铜组饲料铜含量分别为110,220,330mg/kg,试验至60日龄结束,来探讨高铜日粮对肉鸡肝线粒体膜通透性、脂类代谢及肝和肌肉铜含量的影响。结果显示:(1)随着铜浓度的增加和/或饲养日龄的延长,线粒体膜通透性转换孔(Mitochondrial permeability transition pore,MPTP)逐渐扩大(P0.05);(2)血清总胆固醇(T-Ch)含量110,220mg/kg铜添加组低于对照组(P0.05),330mg/kg铜添加组极显著低于对照组(P0.01),而甘油三酯(TG)的含量在各高铜组均极显著低于对照组(P0.01);(3)与对照组相比,肝脏铜含量110,220mg/kg铜添加组有所增加(P0.05),330mg/kg铜添加组极显著增加(P0.01),肌肉中铜含量在各高铜组均极显著高于对照组(P0.01)。这说明高铜可造成肝线粒体不同程度的肿胀和损伤,改变肉鸡脂类的新陈代谢,明显增加肝和肌肉组织的铜含量。 相似文献
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高铜对雏鸡脑组织氧化状态的影响 总被引：1，自引：1，他引：0
1日龄艾维茵肉鸡健雏360羽,随机均分为6组,分别喂以对照日粮(10.89 mg/kg)和高铜日粮(Cu 100 mg/kg,高铜Ⅰ组;Cu 200 mg/kg,高铜Ⅱ组;Cu 400 mg/kg,高铜Ⅲ组;Cu 600 mg/kg,高铜Ⅳ组;Cu 800 mg/kg,高铜Ⅴ组)6周,观察高铜对脑组织氧化状态的影响.高铜Ⅱ、Ⅲ、Ⅳ和Ⅴ组脑组织MDA含量升高,与对照组比较差异显著或极显著(P<0.05或P<0.01);脑组织铜-锌-超氧化物歧化酶(Cu-Zn-SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性,高铜Ⅰ、Ⅱ组显著或极显著高于对照组(P<0.05或P<0.01),高铜Ⅲ、Ⅳ和Ⅴ组较对照组极显著降低(P<0.01);高铜Ⅱ、Ⅲ、Ⅳ和Ⅴ组脑组织羟自由基活性升高,与对照组比较差异显著或极显著(P<0.05或P<0.01).同时,血清的MDA含量及Cu-Zn-SOD和GSH-Px的活性变化与脑组织一致.结果表明,日粮铜水平在400 mg/kg及以上时,脑组织的抗氧化功能受损. 相似文献
16.
One hundred and eight Texel sired lambs were weaned at 37 days old and allocated three days later to one of two treatments, using two pens per treatment, in order to evaluate lamb hepatic copper (Cu) values. In Treatment 1 (T1) the lambs were confined to pens having recycled plastic slatted flooring, while in Treatment 2 (T2) the lambs were confined to pens having straw bedded floors, with fresh straw added on alternate days. The lambs had ad libitum access to a coarse lamb concentrate. The crude protein (g/kg DM), crude fibre (g/kg DM), total zinc (mg/kg) and background levels of copper (Cu) (mg/kg) and molybdenum (Mo) (mg/kg) of the diet were 17.2, 10.9, 152, 9.7 and 0.92, respectively. The lambs were slaughtered when they reached about 39 kg live weight. Following slaughter, the liver was removed and weighed and the caudal lobe frozen for subsequent Cu analysis. The livers of lambs on the plastic floor treatment had higher liver weights (843 vs. 804 g, S.E.M. 11.0; P < 0.05) and higher hepatic copper (396 vs. 315 mg/kg DM, S.E.M. 18.2; P < 0.05) than those from lambs that were bedded with straw. The Cu accumulation per kg of liver DM per week was estimated to be 8.4 and 13.7 mg for lambs on the straw bedding and plastic slated treatments, respectively. It is concluded that when lambs are housed on plastic slatted flooring, relative to straw bedding, there is a higher accumulation of hepatic copper levels likely to result in a greater risk of Cu toxicity when lambs are reared on all concentrate diets. 相似文献
17.
The effects of copper supplementation on the copper status of 40 late-pregnant Aubrac beef cows grazing a copper-deficient pasture and later fed a marginally deficient diet were studied for five months. They were divided into four equal groups; the control group received no copper supplement, groups 1 and 2 received copper as copper sulphate at 10 and 30 mg/kg of diet dry matter (DM), respectively, for five months, and group 3 received 120 mg/kg of diet dry matter for 10 days. Plasma copper concentration and the activity of erythrocyte superoxide dismutase (eSOD) were measured at the beginning of the experiment, in the cows and calves during weeks 1 and 3 after calving, and in the calves before they were turned out to pasture at a mean (sd) age of 51 (26) days. In spite of the low dietary copper content (4.2 mg/kg of DM), the plasma copper concentration of the control cows increased during the winter. All the copper supplements resulted in normal and similar plasma copper concentrations in the cows after calving, but the concentration decreased slightly between weeks 1 and 3 after calving in the group supplemented for 10 days. The treatments did not affect the eSOD of the cows. The calves born to the four groups showed the same patterns of plasma copper and eSOD. Compared with the cows, the calves had low plasma copper concentrations at week 1 and values in the normal range at week 3; their eSOD was high at weeks 1 and 3 but decreased after week 3. 相似文献
18.
本研究旨在探讨铜和维生素A及其互作效应对内仔鸡生长性能及抗氧化功能的影响.试验选用1日龄艾维茵肉仔鸡448只,随机分为8组,每组4个重复,每个重复14只鸡.采用4×2(铜×维生素A)完全随机设计,饲粮铜的添加量分别为0、8、150、225 mg/kg,维生素A的添加量分别为1 500、5 000 IU/kg,分为2个生长阶段,前期为1～4周龄,后期为5～7周龄.结果表明:1)高铜( 150、225mg/kg)抑制了全期肉仔鸡生长性能,提高了全期血清铜蓝蛋白(Cp)和后期血清总超氧化物歧化酶(T-SOD)活性(P＜0.05).2)5 000 IU/kg维生素A组获得较好的生长性能,提高了全期血清T-SOD活性,降低了前期血清Cp活性(P＜0.05).3)铜和维生素A互作效应对前期体增重、血清Cp和T-SOD活性及全期料重比有显著影响(P＜0.05),且二者间存在互补作用.铜(8 mg/kg)×维生素A(5 000 IU/kg)组与铜(0 mg/kg)×维生素A(5 000 IU/kg)组对前期肉仔鸡获得良好生长性能和提高血清T-SOD活性均有促进作用,但对血清Cp活性有显著降低作用(P＜0.05).由此可见,在基础饲粮铜水平为16～23mg/kg时,铜的适宜添加量为前期8 mg/kg,后期0～8 mg/kg;全期维生素A的适宜添加量为5 000 IU/kg. 相似文献
19.
AIM: To determine changes in serum and liver copper concentrations in postnatal, weaner, yearling, and mature deer after grazing pasture topdressed with copper (Cu) at two rates of application of copper sulphate (CuSO4(.)5H2O), and following oral administration of copper oxide (CuO) wire particles to some of the deer. METHODS: In mid-March 2000 (Year 1), 1.1-ha paddocks (two/treatment) of ryegrass/white clover pasture received either 0 (Control), 6 (Low) or 12 (High) kg CuSO4(.)5H2O /ha applied with 250 kg potash superphosphate/ha. They were grazed by 4-month-old red deer hinds (n=11/treatment) from mid-April 2000 until early March 2001. In mid-March 2001 (Year 2), the pastures were topdressed again as for Year 1, and the original hinds, now yearlings which had grazed as a single group between studies, were returned to their respective treatments in mid-April 2001 and remained on the trial until mid-March 2002. They were mated during April/May. The pastures were also grazed by pregnant mature hinds (n=8/treatment) from mid-May 2001. As the Cu status (i.e. liver Cu concentration) of the yearling hinds on the pasture treated with 6 kg CuSO4(.)5H2O/ha was not significantly different from the untreated animals, in late July 2001 the yearling and mature deer on this treatment were treated orally with 10 g CuO wire particles. The mature hinds calved in November and the yearling hinds in December. Pasture samples were collected at about monthly intervals to determine concentrations of Cu and other minerals. In Year 1, liver biopsies and blood samples were collected at 4-6-weekly intervals for determination of Cu concentrations. In Year 2, samples were collected similarly at 6-12-weekly intervals. Liver biopsies and blood were also collected from progeny, along with milk from their dams. Liveweights were determined at 3-7-monthly intervals, as well as data on calving/mortality rates. RESULTS: Pasture Cu concentrations before the application of CuSO4(.)5H2O were 6-9 mg Cu/kg dry matter (DM) and remained at this level in the untreated Control paddocks throughout the study. In Year 1, 28 days after treatment, pasture Cu concentration was 25 and 35 mg Cu/kg DM for the Low and High treatments, respectively; while at the same time for the same treatments in Year 2 it was 20 and 60 mg/kg DM, respectively. A second 60 mg Cu/kg DM peak also occurred on Day 85 in Year 2 with the High treatment. The pasture Cu concentration returned to 6-9 mg/kg DM, and there were no differences between treatments at Days 80 and 150 in Years 1 and 2, respectively. In Years 1 and 2, the Low treatment had no significant effect on the Cu status of the weaner and yearling hinds, respectively, when compared with that of animals grazing the untreated Control pastures. Weaner (Year 1) and yearling (Year 2) deer on the High treatment had significantly higher mean serum and liver Cu concentrations in the late winter and spring period when compared with those on untreated Control pastures. CuO wire particles increased the mean serum Cu concentration at Days 60 and 180, and liver Cu concentration at Day 60, in yearling hinds. A similar effect was observed in mature hinds. Regardless of Cu treatment, the liver Cu concentration of the 1-4-week-old progeny was markedly greater (p<0.001) than that of their dams, and then decreased significantly until weaning in March. In progeny of treated yearling hinds, but not mature hinds, serum and liver Cu concentrations were significantly higher (p=0.013) than progeny of untreated dams. CONCLUSION: Topdressing pastures with CuSO4(.)5H2O at a rate of 12 kg/ha, but not 6 kg/ha, in mid-March was effective in increasing the Cu status of weanling hinds; while pastures topdressed with 12 kg CuSO4(.)5H2O /ha in mid-March and dosing hinds with 10 g CuO in late July were effective in increasing the Cu status of pregnant hinds, and in the case of the yearling hinds, significantly improved the Cu status of their progeny from birth to weaning. 相似文献