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1.
Rathanon KHEMGAEW Mari OMACHI Tomoe TAKESADA Torrung VETCHAPITAK Hiroyuki SATO Takako TANIGUCHI Naoaki MISAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):889
Papillomatous digital dermatitis (PDD) is a polymicrobial infection causing lameness in dairy cattle. Culture-independent analysis has shown that Treponema phagedenis is present consistently and predominantly in the lesions. However, the pathogenesis of PDD, especially the tissue penetration pathway, has not been examined. In the present study, we investigated whether T. phagedenis strains isolated from PDD produce proteolytic enzyme (s) for disruption of the epithelial cell barrier and have the ability to translocate in polarized normal human epidermal keratinocytes (NHEK) in vitro. Ten strains of T. phagedenis isolated from lesions did not show proteolytic activity on modified skim milk agar, although a human strain of T. denticola used as a control showed such activity. The integrity of tight junctions was monitored by measurement of transepithelial electrical resistance (TER). The TER values after inoculation of the T. phagedenis strains examined did not change during the experimental period; however, apical to basolateral translocation of T. phagedenis was confirmed after 24 hr by microscopy and Treponema-specific PCR. We further confirmed that translocation of T. phagedenis was accelerated by co-inoculation with live T. denticola, but not with heat-killed organisms. Furthermore, tight junction ZO-1 protein was not lost intensity after inoculation with T. phagedenis and the organism was observed in NHEK cells using a florescence microscope. These results suggest that T. phagedenis strains may translocate via a transcellular route in vitro and that the invasion is accelerated by other bacteria, such as T. denticola, producing proteolytic activity. 相似文献
2.
Yusuke TANAKA Keisuke SUGANUMA Kenichi WATANABE Yoshiyasu KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1212
Dourine, caused by infection with Trypanosoma equiperdum, is one of the trypanosomiasis in equids. The clinical course of dourine is long-term, ranging from 1–2 months to several years. Since the pathogenesis of dourine has not yet been elucidated, experimental studies using mouse infection models are needed. Although mice are not susceptible to most T. equiperdum strains, some strains can infect mice. Even in such strains, infected mice develop rapidly transient parasitemia and die within 2–8 days. Therefore, mice experimentally infected with these T. equiperdum strains are not suitable for mouse infection models to analysis the pathogenesis of dourine. A sequential method of isolating parasites from dourine-affected horses and adapting them to in vitro cultures using soft agarose media was recently developed. Various T. equiperdum strains adapted to in vitro conditions have been established using this technique. We used one of these strains, the T. equiperdum IVM-t2 strain. In the present study, T. equiperdum IVM-t2 strain inoculated mice developed periodic parasitemia during the experimental period of 60 days. Histopathologically, vaginitis and dermatitis were observed. These findings were comparable to those of dourine-affected horses. Therefore, mice infected with T. equiperdum IVM-t2 strain may be a valuable tool for pathological, immunological, and parasitological in vivo research, and will contribute to investigations on the mechanisms underlying the disease process and the host-parasite relationship. 相似文献
3.
Yamato SAJIKI Satoru KONNAI Reiko NAGATA Satoko KAWAJI Hayato NAKAMURA Sotaro FUJISAWA Tomohiro OKAGAWA Naoya MAEKAWA Yukinari KATO Yasuhiko SUZUKI Shiro MURATA Yasuyuki MORI Kazuhiko OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):162
Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne’s disease. 相似文献
4.
Patrícia M Oba Meredith Q Carroll Celeste Alexander Amy J Somrak Stephanie C J Keating Adrianna M Sage Kelly S Swanson 《Journal of animal science》2021,99(7)
Microbiota plays a prominent role in periodontal disease, but the canine oral microbiota and how dental chews may affect these populations have been poorly studied. We aimed to determine the differences in oral microbiota of adult dogs consuming dental chews compared with control dogs consuming only a diet. Twelve adult female beagle dogs (mean age = 5.31 ± 1.08 yr) were used in a replicated 4 × 4 Latin square design consisting of 28-d periods. Treatments (n = 12/group) included: diet only (CT); diet + Bones & Chews Dental Treats (BC; Chewy, Inc., Dania Beach, FL); diet + Dr. Lyon’s Grain-Free Dental Treats (DL; Dr. Lyon’s, LLC, Dania Beach, FL); and diet + Greenies Dental Treats (GR; Mars Petcare US, Franklin, TN). Each day, one chew was provided 4 h after mealtime. On day 27, breath samples were analyzed for total volatile sulfur compound concentrations using a Halimeter. On day 0 of each period, teeth were cleaned by a veterinary dentist blinded to treatments. Teeth were scored for plaque, calculus, and gingivitis by the same veterinary dentist on day 28 of each period. After scoring, salivary (SAL), subgingival (SUB), and supragingival (SUP) samples were collected for microbiota analysis using Illumina MiSeq. All data were analyzed using SAS (version 9.4) using the Mixed Models procedure, with P < 0.05 considered significant. All dogs consuming chews had lower calculus coverage and thickness, pocket depth and bleeding, plaque thickness, and halitosis compared with CT. In all sites of collection, CT dogs had a higher relative abundance of one or more potentially pathogenic bacteria (Porphyromonas, Anaerovorax, Desulfomicrobium, Tannerella, and Treponema) and lower relative abundance of one or more genera associated with oral health (Neisseria, Corynebacterium, Capnocytophaga, Actinomyces, Lautropia, Bergeyella, and Moraxella) than those fed chews. DL reduced Porphyromonas in SUP and SUB samples. DL and GR reduced Treponema in SUP samples. DL increased Corynebacterium in all sites of collection. BC increased Corynebacterium in SAL samples. DL and GR increased Neisseria in SAL samples. DL increased Actinomyces in the SUB sample. GR increased Actinomyces in SAL samples. Our results suggest that the dental chews tested in this study may aid in reducing periodontal disease risk in dogs by beneficially shifting the microbiota inhabiting plaque and saliva of a dog’s oral cavity. These shifts occurred over a short period of time and were correlated with improved oral health scores. 相似文献
5.
Sabri A. Rahman Kuan H. Khor Siti Khairani-Bejo Seng F. Lau Mazlina Mazlan Azri Roslan Soon H. Goh 《Journal of veterinary diagnostic investigation》2021,33(5):834
Leptospirosis is a serious bacterial disease that affects both humans and animals. A wide range of symptoms have been described in humans; the disease in dogs is commonly associated with kidney and/or liver disease. In Malaysia, information about the common serovars infecting dogs is limited. Therefore, we investigated the occurrences of leptospirosis in 124 pet dogs diagnosed with kidney and/or liver disease. Blood, urine, abdominal effusion, and/or kidney and liver were collected from the dogs. Based on microscopic agglutination testing, 53 of 124 (42.7%) dogs were seropositive for leptospiral exposure. Sera were frequently positive to serovars Bataviae (n = 12), Javanica (n = 10), and Icterohaemorrhagiae (n = 10). Direct detection using PCR showed that 42 of 124 (33.9%) of the whole blood and 36 of 113 (31.9%) urine samples were positive for pathogenic Leptospira spp. By PCR, 2 of 23 (9.1%) kidney and 2 of 23 (9.1%) liver were positive for pathogenic Leptospira spp. Abdominal effusion from 4 dogs were PCR-positive for pathogenic Leptospira spp. The species detected were L. interrogans, L. borgpetersenii, L. kirschneri, and L. kmetyi by partial 16S rRNA sequencing. We further identified and characterized 11 Leptospira spp. isolates from 8 dogs as serovars Bataviae, Javanica, and Australis. The mortality rate of the Leptospira-infected dogs was high (18 of 53; 34%). 相似文献
6.
David O. EHIZIBOLO Joshua KAMANI Peter O. EHIZIBOLO Kinsley O. EGWU Goni I. DOGO Josiah O. SALAMI-SHINABA 《Journal of Equine Science》2012,23(1):1-4
This study was conducted to determine the prevalence and significance of parasites of
horses in northern Nigeria. Blood and faecal samples were randomly collected from 243
horses from different stables in some states of northern Nigeria for laboratory analyses.
Fifty-seven horses (23.5%) were found infected with parasites. The hemoparasites detected,
21 (8.6%), include Theileria equi, Babesia caballi, Trypanosoma vivax and Trypanosoma
evansi. The endoparasites encountered, 29 (11.9%) were Strongylus spp., Strongyloides
spp., Oxyuris equi, Parascaris equorum, Paragonimus spp. and Dicrocoelium spp., 3 (1.2%)
was Eimeria spp. Four horses (1.6%) had mixed infection of hemo- and endoparasites. This
preliminary finding shows that parasitism is a problem in the horse stables examined, and
calls for proper stable hygiene, routine tick control and regular deworming programme. 相似文献
7.
Shotaro KUBO Morihiro TATENO Yasuaki ICHIKAWA Yasuyuki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1275-1279
Tick-borne diseases are often encountered in canine clinical practice. In the present
study, a molecular epidemiological survey of dogs in Japan was conducted to understand the
prevalence and geographical distribution of Babesia spp.,
Hepatozoon spp., Ehrlichia spp. and
Anaplasma spp. Pathogen-derived DNA in blood samples obtained from 722
dogs with a history of exposure to ticks and/or fleas was examined by PCR. The prevalence
of Babesia gibsoni, Babesia odocoilei-like species,
Hepatozoon canis and Ehrlichia
spp./Anaplasma spp. was 2.4% (16/722), 0.1% (1/722), 2.5% (18/722) and
1.5% (11/722), respectively. While B. gibsoni and
Ehrlichia spp./Anaplasma spp. were detected in the
western part of Japan, H. canis was detected in Tohoku area in addition
to western and central parts of Japan. 相似文献
8.
Fitrine EKAWASTI Kazuya KITAGAWA Hiroshi DOMAE April Hari WARDHANA Junki NAGASAWA Tomoyuki SHIBAHARA Masaharu TOKORO Kazumi SASAI Makoto MATSUBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1240
Oocysts of Isospora sp. were detected in the feces of a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) kept at a zoo in Ishikawa, Japan. Phylogenetic analysis placed the sequence in the cluster of Isospora spp. isolated from reptiles. Based on a comparison of morphological data of ten previously reported Isospora species from the Chamaeleonidae family, this isolate was morphologically similar to I. jaracimrmani, which has been considered to be a virulent species. This case study suggests the possibility that species of Isospora might not always cause disease because the animal that shed these oocysts showed no symptoms for more than two months. 相似文献
9.
Francili A. Molossi Bianca S. de Cecco Camila B. Pohl Rogrio B. Borges Luciana Sonne Saulo P. Pavarini David Driemeier 《Journal of veterinary diagnostic investigation》2021,33(4):677
We determined the prevalence of diseases and pathogens associated with mortality in beef cattle in the State of Rio Grande do Sul, Brazil, based on pathology laboratory submissions. Postmortem examinations were conducted on 1,277 beef cattle that died between 2008 and 2018. Information regarding age, time of the year, breed, and regional location were analyzed statistically. Most cattle were from the surrounding region of Porto Alegre, and 78.7% of the analyzed cases had diagnostic value. The diagnostic category with most cases was infectious and/or parasitic diseases (60%), followed by toxic and toxicoinfectious (25%). Most cases occurred in the fall. Major disease conditions identified included hemoprotozoal infection (18.2%), rabies (8.2%), and plant intoxications by Senecio spp. (8.5%) and Pteridium arachnoideum (4.6%). Hemoprotozoal infection occurred at a higher frequency in young cattle, mainly in animals up to 1 y old. Intoxication by Senecio spp. was more frequent in cattle 2–3 y old. 相似文献
10.
Peru Gopal BISWAS Yuma OHARI Uday Kumar MOHANTA Tadashi ITAGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):666
We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high identity with that of A. columbae. However, the ITS1 sequences were available for designing PCR primers distinguishable between Ascaridia galli and Heterakis spp. Bangladesh isolates of A. galli constituted a monophyletic clade along with other geographical isolates in the cytochrome c oxidase subunit I (COI) phylogenetic tree, however, we could not clarify the phylogenetic relationships between A. galli and other Ascaridia spp., because their available sequences in GenBank were very few. The developed PCR method using DNA from A. galli and Heterakis spp. eggs would enable differential diagnosis of the individual infections in the future. 相似文献
11.
Franziska Schott Karolin Hoffmann Eleonora Sarno Patrick D. Bangerter Roger Stephan Gudrun Overesch Michael Haessig Xaver Sidler Robert Graage 《Journal of veterinary diagnostic investigation》2021,33(4):655
The use of oral fluid (OF) to detect zoonotic pathogens in pigs has been only scarcely assessed. We evaluated OF as a potential specimen for detection by culture of methicillin-resistant Staphylococcus aureus (MRSA) and Yersinia enterocolitica, and the detection of antibodies against Salmonella spp. and hepatitis E virus (HEV) using commercial ELISAs. Samples from 33 pig farms were collected at the beginning and end of the fattening period. Results of the OF samples were compared with the results of serum samples and nasal swabs from individual pigs and pen floor fecal samples, using the Cohen kappa (κ) and the McNemar test. For Salmonella spp. antibodies, OF samples were negative, although the corresponding serum samples were positive. The detection of HEV antibodies in sera and OF had agreement at the first sampling, and poor and significant agreement at the second sampling (κ = 0.185, McNemar p = 0.238; κ = 0.088, McNemar p < 0.001). At both sampling times, the detection of MRSA in nasal swabs and OF showed agreement (κ = 0.466, McNemar p = 0.077; κ = 0.603, McNemar p = 1); agreement was seen for the detection of Y. enterocolitica in fecal and OF samples (κ = 0.012, McNemar p = 0.868; κ = 0.082, McNemar p = 0.061, respectively). According to the McNemar test, the use of pen-based OFs is more feasible for the detection of MRSA and Y. enterocolitica by culture than is detection of antibodies by commercial ELISA. 相似文献
12.
Nicole R. Furst James C. Colee Elizabeth W. Howerth Lisa L. Farina 《Journal of veterinary diagnostic investigation》2021,33(4):648
Inclusion bodies (IBs) and multinucleate cells can be associated with viral infections; however, IBs and multinucleate cells have been described in normal tissue and with non-viral disease processes in multiple species. We examined fundic stomach from 50 callitrichids histologically for bi- and multinucleate parietal cells and cytoplasmic IBs in gastric epithelial cells. Callitrichids represented included 6 genera: Saguinus (4 spp.), Leontopithecus (1 sp.), Mico (3 spp.), Cebuella (1 sp.), Callithrix (1 sp.), Callimico (1 sp.), and 13 unspecified marmosets. Gastric epithelial IBs were present in 46 of 47 (98%) of the callitrichids from which the stomach was sufficiently well preserved to identify IBs. Cytoplasmic IBs were identified in gastric surface pit epithelial cells (43 of 44, 98%), mucous neck cells (43 of 44, 98%), parietal cells (43 of 44, 98%), and chief cells (43 of 44, 98%). The IBs were eosinophilic, ovoid, round, elongate, or variably indented, sometimes slightly refractile, and 1–6 × 1–13 µm. IBs were sometimes perinuclear and molded around the nucleus. Electron microscopy of the gastric epithelium of one marmoset indicated that IBs were composed of intermediate filaments. The IBs did not stain with immunohistochemical markers for cytokeratin AE1/AE3 or vimentin. Binucleate parietal cells were found in 49 of 50 (98%) callitrichids, and multinucleate parietal cells were observed in 40 of 49 (82%) callitrichids. Gastric epithelial cytoplasmic IBs and bi- and multinucleate parietal cells are likely a normal finding in callitrichids, and, to our knowledge, have not been reported previously. 相似文献
13.
Viviana Gonzalez-Astudillo Matthew F. Sheley Francisco A. Uzal Mauricio A. Navarro 《Journal of veterinary diagnostic investigation》2021,33(4):721
Cryptosporidiosis is an intestinal protozoal disease of public health importance caused by Cryptosporidium spp. Despite the high synanthropism of raccoons, studies describing the pathology of Cryptosporidium spp. infections in this species are lacking. Therefore, we characterized the pathology of cryptosporidiosis in 2 juvenile raccoons. In addition, we conducted a retrospective search of the database of the California Animal Health and Food Safety laboratory for 1990–2019 and found 6 additional cases of cryptosporidiosis in raccoons. Sequencing of cryptosporidia was performed in one autopsied raccoon, and PCR on formalin-fixed, paraffin-embedded tissues in archived cases. The Cryptosporidium skunk genotype (CSkG), a strain of zoonotic relevance, was detected in 6 of 8 cases (75%). Frequently, cryptosporidiosis was associated with enteritis, eosinophilic infiltrates, villus atrophy or blunting and/or fusion, and crypt abscesses or necrosis. In 7 of the 8 cases, there was confirmed concurrent coinfection with canine distemper virus; 1 case was coinfected with canine parvovirus. Although crypt necrosis is considered a classic lesion of canine parvoviral infection in mesocarnivores and not a hallmark of cryptosporidiosis, results suggest that canine distemper virus is capable of mimicking such lesions in combination with cryptosporidia and immunosuppression. 相似文献
14.
Carlos E. Fidelis Manoela Franke Letícia C. R. de Abreu Tomasz Jagielski Mrcio G. Ribeiro Marcos V. dos Santos Juliano L. Gonalves 《Journal of veterinary diagnostic investigation》2021,33(6):1168
We evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of São Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases. 相似文献
15.
Sara Marques Eliane Silva Júlio Carvalheira Gertrude Thompson 《Research in veterinary science》2010,89(1):5-9
Bovine mastitis associated with Prototheca is considered a rare pathology, but is increasing in prevalence all over the world and therefore becoming more relevant to the dairy industry. The biochemical characterization of 47 Prototheca isolates retrieved from mastitic milk was performed in this study using API 20C Aux and two BBL Crystal Kits, followed by an analysis with InforBio software. The usage of this methodology, allowed the identification of discriminative phenotypic characteristics for the strains tested. The differential-character-finding algorithm used by this software permitted the identification of new phenotypic characteristics to discriminate between Prototheca zopfii, P. blaschkeae and P. wickerhamii, such as, citrate, phosphorycholine and arabinoside. The main objective of this study was to determine new phenotypic characteristics that allowed a better characterization of Prototheca spp. Usage of recent bioinformatic tools improved the analyses of several features that are important for a better characterization of Prototheca spp. 相似文献
16.
Justice Opare ODOI Michiyo SUGIYAMA Yuko KITAMURA Akiko SUDO Tsutomu OMATSU Tetsuo ASAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1191
Wild birds are recognized as disseminators of antimicrobial-resistant (AMR) bacteria into the environment. Here, we isolated AMR indicator bacteria from 198 Great Cormorant cloacal swabs collected in Shiga (n=90), Oita (n=52), Gifu (n=29), and Gunma (n=27) Prefectures, Japan, in 2018 and 2019. In total, 198 Aeromonas spp. and 194 Escherichia spp. were isolated, and their antimicrobial susceptibility was examined. Aeromonas spp. were resistant to colistin (8.6%), nalidixic acid (4%), and other antimicrobials (<2%), with 3.0% positivity for mcr-3. Escherichia spp. showed resistance to colistin (3.1%), ampicillin (2.6%), tetracycline (2.1%), and other antimicrobials (<2%). This study shows the presence of AMR bacteria in Great Cormorants, indicating that these birds potentially disseminate AMR bacteria. 相似文献
17.
Jongsung LEE Sangil SUH Ran CHOI Changbaig HYUN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1677-1680
This study evaluated anesthesia quality, degree of analgesia and cardiorespiratory
parameters after intramuscular (IM) injection of a combination of butorphanol (0.1 mg/kg),
medetomidine (10 µg/kg) and alfaxalone (1.5 mg/kg) in ten healthy adult
Beagle dogs. Rectal temperature (T), heart rate (HR), respiratory rate
(fR), arterial pressure, arterial blood gases and M-mode
echocardiographic left ventricular (LV) indices were measured before drug administration
and every 10 min thereafter until extubation. Mean duration of anesthesia, recovery and
analgesia were 89 ± 17, 6 ± 1 and 80 ± 12 min. HR, fR, partial
pressure of arterial CO2 and O2, arterial pressure, and LV
contractility were significantly altered during anesthesia. IM administration of the drug
combination provided acceptable anesthesia, but produced substantial cardiorespiratory
suppression. 相似文献
18.
Marta Campillo Sionagh H. Smith David L. Gally Tanja Opriessnig 《Journal of veterinary diagnostic investigation》2021,33(4):621
Lawsonia intracellularis is an obligate intracellular bacterium associated with enteric disease in pigs. Clinical signs include weight loss, diarrhea, and, in some cases, sudden death. The hallmark lesion is the thickening of the intestinal mucosa caused by increased epithelial cell replication, known as proliferative enteropathy. The immune response to L. intracellularis is not well defined, and detection of the infection, especially in the early stages, is still a significant challenge. We review here the main approaches used to identify this important but poorly understood pathogen. Detection of L. intracellularis infection as the cause of clinical disease is confounded by the high prevalence of the pathogen in many countries and that several other pathogens can produce similar clinical signs. A single L. intracellularis–specific ELISA and several amplification assays are available commercially to aid detection and surveillance, although histopathology remains the primary way to reach a conclusive diagnosis. There are major gaps in our understanding of L. intracellularis pathogenesis, especially how the host responds to infection and the factors that drive infection toward different clinical outcomes. Knowledge of pathogenesis will increase the predictive value of antemortem tests to guide appropriate interventions, including identification and treatment of subclinically affected pigs in the early stages of disease, given that this important manifestation reduces pig productivity and contributes to the economic burden of L. intracellularis worldwide. 相似文献
19.
Alessandra MMG de Castro Taís F Cruz Vanessa R Salgado Tatiana M Kanashiro Karen L Ferrari Jo?o P Araujo Jr Paulo E Brand?o Leonardo J Richtzenhain 《Acta veterinaria Scandinavica》2012,54(1):29
Background
Porcine circovirus type 2 (PCV2) has been associated with several disease complexes, including reproductive failure. The aim of this study was to identify the subtypes of PCV2 that are associated with reproductive failure in pigs from the State of São Paulo, Brazil and to investigate co-infections with other infectious organisms.Findings
Samples of 168 aborted foetuses or mummified foetuses from five farrow-to-finish swine farms known to be infected with PCV2 and located in the State of São Paulo were tested for PCV2 by polymerase chain reaction (PCR). Positive samples were additionally tested for porcine parvovirus (PPV), Leptospira spp. and Brucella spp. by PCR. PCV2 was detected in 18 of the samples (10.7%). PPV, Brucella spp. and Leptospira spp were found in 2, 10 and 0 cases, respectively. Eleven PCV2 strains were sequenced and determined to be either genotype 2a (n = 1) or 2b (n = 10).Conclusions
The findings indicate that the frequency of PCV2 infections in aborted porcine foetuses from the State of São Paulo is rather low (10.7%) and that co-infection with other pathogens is common and may be involved in PCV2 associated reproductive failure. No repeatable, characteristic amino acid motifs for regions of the PCV2 capsid protein seemed to be associated with abortion in sows. 相似文献20.
Shuhei HIDAKA Mitsutoshi KOBAYASHI Kunihide ANDO Yoshikazu FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):829-835
Lomefloxacin is a broad-spectrum fluoroquinolone antibiotic used for the treatment of
bacterial extraocular disease. This study investigated the efficacy and safety of
lomefloxacin eye drops for bacterial extraocular disease in horses. Lomefloxacin
ophthalmic solution (0.3%) was instilled three times daily for 2–5 days in 65 horses
diagnosed with bacterial extraocular disease based on clinical findings. Clinical
observations and bacteriological examinations were performed at the start of treatment, 2
and 5 days after the start of treatment, and at the discontinuation or termination of
treatment. Of the 65 horses, 64 were positive for bacteria, and 22 bacterial genera and 47
bacterial species were identified. The efficacy of lomefloxacin was evaluated in 63
horses; one horse with a negative culture and another with suspected bacterial
contamination were excluded. Lomefloxacin was considered to be clinically effective in 54
horses. The major bacterial species identified were Staphylococcus
aureus, Streptococcus equi subsp. zooepidemicus,
Acinetobacter lwoffii, Staphylococcus xylosus,
Staphylococcus vitulinus, Enterobacter agglomerans,
Flavimonas oryzihabitans and Staphylococcus sciuri,
with a cumulative disappearance rate of 80% or more at the termination of instillation.
Excluding one horse that did not undergo a bacteriological examination, the remaining 62
horses were assessed for bacteriological outcome. Full or partial bacterial clearance was
detected in 95% or more of the 62 horses. One of the 65 horses reported adverse events
that had no causal relation with the eye drops. Our results showed that lomefloxacin is
safe and effective for the treatment of bacterial extraocular disease in horses. 相似文献