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1.
A study on ectoparasites of small ruminants in three selected agro-ecological sites of Tigray Region, Ethiopia disclosed an overall prevalence of 55.5% and 58% in each examined 750 sheep and goats, respectively. In the sheep population, Melophagus ovinus (19.1%), tick infestations (16%), Damalinia ovis (15.3%), Linognathus africanus (11.5%), and Ctenocephalides felis (9%) were the major ectoparasites. The major ectoparasites identified in goats were tick infestations (29.7%), L. africanus (27.9%), Sarcoptes scabiei var. caprae (12.5%), C. felis (11.1%), and Demodex caprae (6.8%). In sheep, there was a statistically significant difference (P < 0.001) in the prevalence of Damalinia ovis, M. ovinus, L. africanus, and ticks between midland and highland. In goats, the risk of Sarcoptes scabiei var. caprae infestation in midland (odds ratio (OR) = 17.2, P < 0.001) and lowland (OR = 5.2, P < 0.001) was 17.2 times and 5.2 times, respectively, higher than the highland. Favorable climatic conditions, backward level of management, poor level of consciousness and awareness of farmers, and weak animal health extension services are believed to have contributed for widespread distribution and occurrences of ectoparasites. The growing threat of ectoparasites to small ruminant production and the tanning industry needs well-coordinated and urgent control intervention.  相似文献   

2.
Sarcoptes scabiei infestation was diagnosed in 278 sheep from 12 fatling flocks. The sheep presented crusted skin lesions initially appear on the lips or nostrils, the lesion on nostrils also extend towards around the eyes, the supraorbital fossae and in some cases over entire face. The infested male lambs by mating behavior in fatling flocks caused transmitting the infection to fat tail area and scrutum. Histopathological study of lesions demonstrated marked acanthosis, hyperkeratosis, parakeratosis. Tunnels could be observed in the hyperkeratotic stratum corneum and mite segments were located mainly in the stratum corneum and also in the stratum granulosum. Attempts to eliminate S. scabiei var. ovis were made in 3 naturally infested sheep herds, by two dippings with two weeks interval with Amitraz, Cypermetrin and Prompetamphos. The results of this study indicate that acaricidal treatment of S. scabiei var. ovis in 3 naturally infested herds was successful, but a few cases of reinfestation were found in each treated group.  相似文献   

3.
Summary A survey on mange mite infestations in the aetiology of skin lesions in goats in Peninsular Malaysia is described. Skin lesions were observed in 25 (93%) of the 27 goat farms investigated. Mange mites were found in 22 (88%) of these goat herds.Chorioptes texanus was found in 20·7%,Psoroptes cuniculi in 19·3%,Sarcoptes scabiei in 18·6% andDemodex canis var.caprae in 0·4% of the samples, taken from the skin lesions. Thirteen cases of generalised mange were diagnosed, from which 9 were caused byS. scabiei, 2 byP. cuniculi and one byC. texanus. All other cases had more or less localised lesions. No significant differences could be found in incidence and distribution of skin lesions between age classes.
Encuesta Sobre La Importancia De Sarna En La Etiologia De Lesiones De Piel En Caprinos De La Peninsula De Malasia
Resumen Se describe una investigación sobre la importancia de infestaciones por ácaros de la sarna en la etiología de la patología cutánea de cabras de la peninsula de Malasia. Se observaron lesiones cutáneas en 25 (93%) de los rebaños investigados. Se encontraron ácaros de la sarna en 22 (88%) de estos rebaños.Chorioptes texanus se encuentró en 20·7%,Psoroptes cuniculi en 19·3%,Sarcoptes scabiei en 18·6% yDemodex canis var.caprae en 0·4% de los muestros sacados de lesiones cutáneas. Se diagnosticaron trece casos de sarna generalizada, nueve de los cuales con causaS. scabiei, dosP. cuniculi y unoC. texanus. Los otros fueron todos más o menos localizados. No se pudo demostrar ninguna diferencia significativa ni en la incidencia ni en la localización de las lesiones cutáneas entre las varias categorías de edad.
Etude Sur L'importance De La Gale Dans L'ethiologie Des Dermatites (Lesions De La Peau) Chez Les Caprines En Malaisie Peninsulaire
Résumé Une enquête sur l'importance d'infestations à acariens dans l'étiologie d'affections cutanées des chèvres en Malaisie péninsulaire est décrite. Des lésions cutanées ont été observées dans 25 (93%) des 27 élevages de chèvres visités. Des acariens ont été mis en évidence dans 22 (88%) de ces élevages.Chorioptes texanus a été trouvé dans 20,7%,Psoroptes cuniculi dans 19,3%,Sarcoptes scabiei dans 18,6% etDemodex canis var.caprae dans 0,4% des échantillons prévelés des lésions cutanées. Treize cas de gale généralisée ont été observés, dont neuf étaient causés parS. scabiei, deux parP. cuniculi et un parC. texanus. Les autres cas étaient plus ou moins localisés. Il n'y avaient pas de différences significatives dans la prévalence ni dans la distribution des lésions cutanées entre les différentes catégories d'âge des chèvres.
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4.
Worldwide, sarcoptic mange in cats is seldom reported, and then only in sporadic individual cases. We describe an epidemic in a household with a dog and 25 cats. From September 2002, the dog was repeatedly treated with ivermectin for sarcoptic mange. The diagnosis was confirmed by skin scrapings. Fifteen months later, cats from the same household were diagnosed with severe sarcoptic mange. Twenty‐one of the cats were euthanized and necropsies were performed. Skin samples were taken from all cats from different body sites for histology, and skin scrapings were examined for ectoparasites. Samples for bacterial and dermatophyte culture were taken from six cats. Smears for cytology were made from lesions on four cats with severe mange. Sera from 21 cats and the dog were analysed for specific antibodies to Sarcoptesscabiei. Molecular characterizations of six individual mites were done. Large numbers of S.scabiei were isolated from the infected skin of most of the cats. Two‐thirds of the cats showed skin lesions compatible with chronic sarcoptic mange. Macroscopically, internal organs exhibited no obvious pathology. Yeast organisms and coccoid bacteria were found in the smears; penicillinase‐negative Staphylococcus aureus was isolated from all samples and Malassezia pachydermatis was identified from four cats. Sarcoptes scabiei was seen histologically in all cats showing chronic skin lesions. No other ectoparasites were found. All analysed cats had specific antibodies against S. scabiei. Twenty‐one cats tested negatively for FeLV and FIV. The mites had DNA sequences identical to S. scabiei from naturally infected dogs and Swedish wildlife. Funding: Self‐funded.  相似文献   

5.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

6.

A total of 1000 clinically healthy small ruminants comprising 500 sheep and 500 goats from five districts within Riyadh Province in Saudi Arabia were investigated by routine Giemsa staining for hematozoan parasites. Out of these, 100 sheep and 95 goat samples were investigated by PCR using three pairs of hemoprotozoan-specific primers. Based on microscopic examination, 33.2% of sheep and 25.2% of goats were found infected with hemoprotozoan parasites, while PCR detected hematozoan infection in 46% of sheep and 33.7% of goats. Extensive molecular characterization of hematozoan infection using six pairs of species-specific primers revealed the dominance of Theileria ovis, rather than any other species, which is recorded for the first time in small ruminants in Saudi Arabia. Prevalence of T. ovis in sheep and goats was found to be the highest in Riyadh (32, 48%) followed by AL-Kharj (31, 35%), Ad-Dawadimi (31, 33%), AL-Majmaah (15, 27%), and Rumah (17, 23%). The highest parasite prevalence was recorded in the 3 years of age and >?4 years of age ruminants, while the lowest prevalence was recorded in <?1 year of age ruminants. No noticeable differences in parasite prevalence between male or female ruminants were recorded. Partial sequencing of 18S rRNA gene revealed the infection of the studied ruminants with four new isolates of T. ovis. Further characterization of the pathogenicity and the clinical effects of these T. ovis isolates in sheep and goats is highly needed. The current results can be helpful in protecting and improving livestock industry in the countries that depend on a high number of small ruminants.

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7.
Examination of possible transmission of the Irish strain of the sheep scab mite (Psoroptes ovis) of ovine origin between host species involved calves and goats being placed with infected sheep and also directly challenged with live mites. Although mites remained on recipient species for up to a week, they failed to produce disease in either of these species. Calves and goats that had live mites placed on them likewise failed to infect sheep with which they were housed.  相似文献   

8.
A survey aimed at determining the status of ticks in cattle, sheep and goats in Bedelle district (Southwestern Ethiopia) was carried out from November 2007 to April 2008. Out of the total 330 animals examined, 314 (95.2%) were found to be infested (harbouring of at least a single tick). High rates of infestations were recorded across all three host species. Factors like month of collection, age and sex of host species did not show significant association with infestation rates. A total of 5,507 ticks belonging to three genera (i.e. Amblyomma, Boophilus and Rhipicephalus) were collected during the study period of which six species were identified. The species of ticks encountered comprise of Amblyomma cohaerens (44.1%), Amblyomma variegatum (13.8%), Amblyomma lepidum (1.2%), Boophilus decoloratus (24.9%), Rhipicephalus evertsi evertsi (14.3%) and Rhipicephalus lunulatus (1.7%). As about 86.7% (4,772) of the ticks were collected from cattle, it was recognised that this could be an evidence of host preference where cattle are serving as principal host while sheep and goats serve as alternative hosts. The role of ecological factors and little attention paid by livestock owners for treatment of animals against ticks are suggested to result in the abundance of ticks. Acaricide spraying should be strategically applied to control ticks. Better result could be achieved if an emphasis is being put on spraying cattle. Studies on tick-borne diseases, involvement of wildlife species as well as related factors are recommended as they may provide a valuable basis for designing and launching an all-round control programme in the country.  相似文献   

9.
A study was conducted to determine the prevalence, larval burden, and associated gross pathological lesions of Oestrus ovis in sheep and goats slaughtered at Luna export abattoir in Central Oromia from November 2007 to March 2008. For this purpose, a total of heads of 431 goats and 369 sheep were thoroughly examined for the presence of first (L1), second (L2), and third (L3) larval stages according to standard procedures. O. ovis larvae were detected in 349(94.6%) sheep and 381(88.4%) goats. All three larval instars were observed in each study months. Statistically significant variation (χ 2 = 29.2676, df = 6, P < 0.05) was observed in the prevalence of O. ovis among small ruminants of different origins. Likewise, statistically significant (χ 2 = 68.3, df = 4, P < 0.05) difference was recorded in the prevalence of O. ovis in sheep and goats among different study months. The overall monthly prevalence ranged from 77.7% in November to 98.8% in March. The prevalence of O. ovis in small ruminants of less than 1 year of age was significantly (χ 2 = 8, df = 1, P < 0.05) higher than those with greater than 1 year of age. An overall proportion of 33.8%, 40.1%, and 26.1% were recorded for L1, L2 and L3, respectively. Whereas 6.8 monthly mean larval burden per individual infested animal was noticed. Out of the total infested heads in goats, 33.6% had catarrhal discharges, 16.8% purulent exudates, 64.83% rhinitis, 68.77% sinusitis, 14.2% pharyngitis, and 9.2% bloody exudates. Similarly, of the total infested heads of sheep, 18.9% purulent exudates, 80.8% rhinitis, 71.9% sinusitis, 13.5% pharyngitis, and 7.7% bloody exudates gross lesions were recorded.  相似文献   

10.
In this work the clinical evolution and the specific serum IgG and IgE antibody responses in sheep after primary (n = 10) and secondary (n = 4) experimental challenges with the mange mite Sarcoptes scabiei var. ovis were studied. The primary infection was characterized by the development of mange lesions in all sheep, a detection of live S. scabiei mites in 70% skin scrapings taken in week 10 post-challenge (PC), strongly raised and sustained specific IgG levels and a more moderate but continuous rise in specific IgE levels. Seroconversion was detected for IgG and IgE by ELISA in 90% and 60% of the sheep in week 8 PC, respectively. By Western-blotting (WB), ten IgG-reactive bands (36–120 kDa) and four IgE-reactive bands (90–180 kDa) were observed in week 8 PC. Following the secondary challenge the ewes developed a smaller area of mange lesion than that seen following primary challenge and live S. scabiei mites were not detected in skin scrapings collected in week 8 PC, suggesting that sheep had developed immunity to re-infection. Compared to primary infection, the specific IgG secondary antibody levels were transient, but in contrast there was an anamnestic IgE response, resulting in an elicitation of specific serum IgE levels in week 2 PC significantly higher than those demonstrated after primary infection. WB analysis revealed one additional IgG-reactive band (180 kDa) and no additional IgE-reactive bands. Determining the immunodiagnostic or vaccination value of the IgG-reactive antigens and IgE-reactive allergens detected requires further studies.  相似文献   

11.
In Ethiopia, ticks and tick-borne diseases are widely distributed and contribute to important economic losses. Several studies investigated the prevalence and species composition of ticks infesting ruminants; however, data on tick-borne pathogens are still scarce. During the study period from October 2010 to April 2011, a total of 1,246 adult ticks and 264 nymphs were collected from 267 cattle and 45 sheep in Bako District, western Oromia, Ethiopia. The study showed infestation of 228/267 (85.4 %) cattle and 35/45 (77.8 %) sheep with adult ticks. Overall, eight tick species, belonging to three genera (Amblyomma, Rhipicephalus, Hyalomma), were identified and Amblyomma cohaerens (n?=?577), Rhipicephalus evertsi evertsi (n?=?290), Rhipicephalus (Boophilus) decoloratus (n?=?287), and Amblyomma variegatum (n?=?85) were the more prevalent species. A statistically significant host preference in A. cohaerens for cattle and R. evertsi evertsi for sheep was noticed. Molecular detection of piroplasms, performed only for adult ticks of two species of the genus Rhipicephalus (R. evertsi evertsi and R. decoloratus), revealed an overall prevalence of 4 % (8/202) Theileria buffeli/sergenti/orientalis, 0.5 % (1/202) Theileria velifera, and 2 % (4/202) Theileria ovis. The study showed that tick infestation prevalence is considerably high in both cattle and sheep of the area, but with a low intensity of tick burden and a moderate circulation of mildly pathogenic piroplasm species.  相似文献   

12.
Using qPCR, standard PCR and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from Souk Ahras, Algeria.Borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. Anaplasma ovis was screened in (38/73, 52%) Rhipicephalus bursa and (5/22, 22.7%) R. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively.Coxiella burnetii tested positive in R. bursa (4/73, 5.5%) and (7/120, 5.8%) blood of sheep and (2/120, 1.7%) goats. Theileria ovis was detected in (50/147, 34%) R. bursa and (3/22, 13.6%) R. turanicus and in (64/120, 53.3%) blood of sheep and (25/120, 20.8%) goats. Babesia ovis was screened positive in (23/147, 15.6%) R. bursa and (7/48, 14.6%) R. turanicus.Our findings expand knowledge about the repertoire of tick-borne microorganisms present in ectoparasites and/or the blood of small ruminants in Algeria.  相似文献   

13.
A survey was conducted to identify tick species and determine the prevalence of tick infestation in small ruminants of Miesso District, West Harergie Zone. Collection and identification of the ticks were undertaken from November 2007 to April 2008. All visible individual adult ticks were collected from the body of 328 goats and 40 sheep. The prevalence of tick infestation in goats and sheep was found to be 89.9% and 87.5%, respectively. In this study, ten species of ticks which grouped under four genera were identified. The most abundant species found in this study were Boophilus decoloratus (60%), Rhipicephalus pulchellus (25.1%), and Amblyomma gemma (11%). Hyalomma dromedarii was the minor species observed on goats. The difference in the prevalence of tick infestation between sheep and goats was not statistically significant (Χ 2   = 0.22, p = 0.63) but found to be statistically significant between male and females (Χ 2  = 9.8, p = 0.003). Attention should be given to the control and prevention of ticks, since they cause sever damage to the skins of small ruminants and thereby reduce the foreign exchange of the country; they also transmit some diseases which can cause sever loss to the productivity of these animals.  相似文献   

14.
When goats in Eastern Tanzania were screened for skin diseases, Dermatophilus congolensis was isolated from the skin lesions in 8 of 484 animals examined. In one severely affected case, the disease was also characterized by histological studies (Gram stain, Giemsa stain and routine HE studies) and electron microscopy. The histological picture was characterized by hyperkeratosis, parakeratosis, acanthosis, folliculitis and an inflammatory cellular reaction involving the epidermis. Gram stain and Giemsa stain revealed longitudinal and transverse branching filaments in the deeper layers of the epidermis. Ultrastructural studies demonstrated D. congolensis in various morphological forms, ranging from filamentous to tuber-shaped structures, mixed with numerous coccoid bodies of variable size. In some instances, the organisms were geometrically arranged in parallel rows of beading and were present in and among the degenerated epithelial cells. Several host cells showed degenerative changes. Ticks present on the goats were Amblyomma variegatum, Rhipicephalus evertsi, Rhipicephalus pravus and Boophilus sp. The clinical signs, pathological lesions, diagnosis, epidemiology and pathogenesis of the disease are discussed.  相似文献   

15.
Although Mycoplasma ovis (formerly Eperythrozoon ovis) has been described in small ruminants worldwide, data on M. ovis in goats remain scarce. Accordingly, the aims of the present study were to i) determine the prevalence of hemoplasmas in goats, ii) identify the tick species parasitizing the animals, and iii) determine factors associated with infection in five dairy and three beef goat farms from the Paraíba State, northeastern Brazil. Blood samples were obtained from 402 goats. Samples were screened for hemoplasmas using a pan-hemoplasma PCR. The positive samples were confirmed by sequencing. An epidemiological questionnaire was given to each farm owner addressing age, gender, and presence of ticks. A total of 158/402 (39.3%) goats were positive for M. ovis by PCR. Sequencing of PCR positive samples has shown ≥99% identity with multiple M. ovis 16S rDNA sequences deposited in GenBank, including M. ovis isolates from humans. Dairy (OR = 2.15; 95% CI: 1.40–3.32%; P = 0.0004) and anemic goats (OR = 2.33; 95% CI: 1.51–3.71%; P = 0.0001) were more likely to be infected than beef and non-anemic animals, respectively. Amblyomma parvum (49/52, 94.23%) and Rhipicephalus microplus (3/52, 5.77%) were the tick species found parasitizing the animals, with no significant association between the presence of ticks and infection by M. ovis (P = 0.1164). This is the first reportedly molecular detection of M. ovis infection in goats from South America. In conclusion, M. ovis is highly prevalent in goats from northeastern Brazil, mainly in dairy animals.  相似文献   

16.
Despite the importance of small ruminants breeding in developing countries, milk/meat productivity remains unsatisfactory. Infectious diseases, such as leptospirosis, brucellosis, and small ruminant lentiviruses (SRLVs), contribute to this scenario. The objective of the present study was to determine the role of each of these diseases in the productivity of small ruminants breeding in Rio de Janeiro, Brazil. In goats, 343 samples were tested for leptospirosis, 560 for Brucella abortus, and 506 for caprine arthritis-encephalitis (CAE), whereas in sheep, 308 samples were tested for leptospirosis, 319 for B. abortus, 374 for Brucella ovis, and 278 for Maedi-Visna (MV). Regarding leptospirosis, 25.9% of goats and 47.4% sheep were seroreactive, with serovar Hardjo the most prevalent in both species. Anti-B. abortus agglutinins were found in 0.7% of all samples, exclusively in goats. In relation to SRLVs, 8.6% of goats and 3.2% of sheep samples were positive for CAE and MV, respectively. Leptospirosis was the major infectious problem in the small ruminants sampled and may contribute to impaired productivity of these animals.  相似文献   

17.
Summary The heads of 1,489 sheep and 320 goats were examined for larvae ofOestrus ovis at 17 abattoirs in northern Libya in July to November 1988. The prevalence ofO. ovis in sheep was 22·6% and in goats it was 18·4 per cent. Up to 14 and 11 larvae were collected from individual sheep and goats respectively. All larvae were recovered from the nasal passages and frontal sinuses, but only second and third instars were seen.
Miasis Causada PorOestrus Ovis En Ovejas Y Cabras Libias
Resumen Durante los meses de julio a noviembre de 1988, se examinaron las cabezas de 1489 ovejas y 320 cabras para detectar larvas deOestrus ovis, en 17 mataderos en el norte de Libia. La prevalencia deO. ovis en ovejas fue de 22·6% y en cabras de 18·4%. Se localizó hasta un máximo de 14 y 11 larvas en cabezas ovinas y caprinas respectivamente. Todas las larvas fueron recuperadas de las fosas nasales y los senos frontales; sólo se encontraron los estadíos larvarios segundo y tercero.
Myiase A Oestrus Ovis Chez Des Ovins Et Caprins En Libye
Résumé Les têtes de 1 489 moutons et de 320 chèvres ont été examinées dans 17 abattoirs de la Libye du nord, de juillet à novembre 1988, pour rechercher des larves d'Oestrus ovis.La prévalence d'Oestrus ovis chez les moutons était de 22,6 p. 100 et chez les chèvres de 18,4 p. 100. Le nombre maximal de larves trouvées par animal a été de 14 chez les moutons et 11 chez les chèvres. Toutes les larves ont été récoltées des conduits nasaux et des sinus frontaux, mais seuls les deuxièmes et troisièmes stades ont été observés.
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18.
Das  M.  Laha  R.  Devi  P.  Bordoloi  R. K.  Naskar  S. 《Tropical animal health and production》2010,42(5):1009-1011
Skin lesions were collected from 15 adult crossbred pigs maintained at the pig farm of ICAR Research Complex for the North-Eastern Hill Region, Umiam, a hilly region of the state of Meghalaya, India. Skin scrapings of these 15 animals were examined by KOH digestion method. Presence of mites were detected in five animals which were indistinguishable from Sarcoptes scabiei var. suis. Infected pigs were treated with a single dose of Ivermectin at 200 ug/kg body weight, subcutaneously. No mites were recovered from treated pigs after a period of 10 days of post-treatment. The presence of S. scabiei var. suis in pigs from this part of the country has never been reported. This infestation has great significance because it affects the growth rate and feed conversion significantly, specially to the grower and weaned pigs. It can be concluded that S. scabiei var. suis infestation is prevalent in hilly region of Meghalaya. The pig producers are generally unaware about the severity of the problem of mite infestation, so precaution should be taken to prevent their valuable pigs from S. scabiei var. suis infestation.  相似文献   

19.
The Apicomplexan parasite Besnoitia caprae infects wild and domestic goats. Knowledge on Besnoitia caprae specially an optimized animal model is sparse. Experimental infections with tachyzoites of BC-Pars obtained from BALB/c mice were conducted in outbred mice to determine the infectivity and LD50 of Besnoitia caprae. Six groups of five mice were intraperitoneally infected with 12.5 × 103, 25 × 103, 5 × 104, 1 × 105 and 2 × 105 tachyzoites and a control inoculum of DMEM, respectively. Although morbidity and mortality were observed in all groups, two mice in the 12.5 × 103 group showed alopecia and skin lesions on 60 days post-infection (DPI). Histopathological and molecular examination of skins confirmed B. caprae infection. The LD50 was calculated as 25 × 103.2 tachyzoites per mouse. The results indicate that outbred BALB/c mice can be used as a suitable model of besnoitiosis and to screen candidate treatments and to test the efficacy of vaccines for besnoitiosis.  相似文献   

20.
Q fever is a zoonotic infection threatening human health, causing abortions in cattle, sheep and goats. Coxiella burnetii (C. burnetii) also causes serious problems such as low birth weight, infertility. This study is the first exemplary for analysis of Q fever around Black Sea region in Turkey. In the study, a total of 270 aborted fetuses (171 cattle, 79 sheep, 20 goats) and 1069 tick samples were aimed to be searched by PCR method. C. burnetii DNA was detected in 8 (2.96 %) of 270 sheep specimens while it could not be found in cattle and goat specimens. 406 sample pools were created from 1069 tick samples (490 male, 579 female) collected from 254 farm animals (187 cattle, 54 sheep, 13 goats) and 11 of these were stated positive. Tick species determined as C. burnetii positive were Hyalomma marginatum, Hyalomma anoliticum excavatum, Hyalomma detritum and Boophilus annulatus. Agent isolation was carried out within embryonated eggs. Agents were stained with Giemsa and was showed. Sequence analysis was performed for TUR/SAM/coxiella_1 (MN917207) isolate and phylogenetic tree was created. This tree, created in compliance with IS1111 transposon gene, did not form different branches in regard to host affiliation (goat, sheep, tick, human) and geographical distribution. As a result, an important zoonotic agent, C. burnetii was diagnosed in sheep aborted fetuses and the infection was proved to have spread among sheep herds in Black Sea region. Besides, 4 separate tick species found in our region hosted the agent and were found important for infection.  相似文献   

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