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1.
This experiment was conducted to investigate the effect of dietary 1α-hydroxycholecalciferol (1α-OH-D3) in calcium (Ca)- and phosphorous (P)-deficient diets on growth performance, carcass characteristics, tibia related parameters, and immune responses of broiler chickens. A total of 280 one-day-old broiler chickens (Ross 308) were assigned to 20 floor pens and 4 dietary treatments with 5 replicates. Dietary treatments consisted of starter diets (starter diet of treatment A: 1% Ca, 0.73% total phosphorus [tP]; starter diet of treatment B: 0.85% Ca, 0.64% tP + 5 μg/kg of 1α-OH-D3; starter diet of treatment C: 0.85% Ca, 0.59% tP + 5 μg/kg of 1α-OH-D3; starter diet of treatment D: 0.85% Ca, 0.54% tP + 5 μg/kg of 1α-OH-D3), grower diets (grower diet of treatment A: 0.86% Ca, 0.68% tP; grower diet of treatment B: 0.73% Ca, 0.59% tP + 5 μg/kg of 1α-OH-D3; grower diet of treatment C: 0.73% Ca, 0.55% tP + 5 μg/kg of 1α-OH-D3; grower diet of treatment D: 0.73% Ca, 0.50% tP + 5 μg/kg of 1α-OH-D3) and finisher diets (finisher diet of treatment A: 0.81% Ca, 0.64% tP; finisher diet of treatment B: 0.68% Ca, 0.56% tP + 5 μg/kg of 1α-OH-D3; finisher diet of treatment C: 0.68% Ca, 0.52% tP + 5 μg/kg of 1α-OH-D3; finisher diet of treatment D: 0.68% Ca, 0.48% tP + 5 μg/kg of 1α-OH-D3). Results showed that body weight gain (BWG) and feed intake (FI) of broilers in treatment B were similar to those of broilers in treatment A at the end of the trial (P < 0.05). Broilers in treatments C and D had lower BWG and FI than those in treatment A during the whole trial (P < 0.05). Feed conversion ratio, carcass traits and relative weight of lymphoid organs were not affected by dietary treatments (P > 0.05). Dietary treatments had no significant effect on antibody titers against Newcastle and Influenza disease viruses as well as sheep red blood cells. Dietary treatments had no significant effects on tibia ash and tibial dyschondroplasia score. Broilers fed Ca-P deficient diets had lower tibia Ca and P than those in treatment A (P < 0.05). In conclusion, results indicated that broilers fed Ca-P deficient diets supplemented with 5 μg/kg 1α-OH-D3 failed to achieve the same tibia Ca and P values as broilers fed nonphytate phosphorus adequate diets.  相似文献   

2.
The present study aimed to evaluate the effects of manganese methionine hydroxyl analog chelated (Mn-MHAC) as a manganese (Mn) source on growth performance and trace element deposition in broilers. A total of 432 Arbor Acres commercial female broilers were fed a basal corn-soybean diet containing Mn at 25.64 mg/kg diet for 10 d. They were then randomly assigned to 6 groups, including a control group (the basal diet), a Mn sulfate group (the basal diet supplemented with Mn at 100 mg/kg diet), and 4 Mn-MHAC groups (the basal diet supplemented with Mn-MHAC at 25, 50, 75 and 100 mg Mn/kg diet, respectively). The results showed that compared with the control group, groups supplemented with Mn-MHAC had a positive effect on BW (quadratic, P = 0.017) and ADG (quadratic, P = 0.017). Moreover, the Mn-MHAC (50 mg Mn/kg diet) group had significantly greater BW and ADG (P < 0.05) compared with the other Mn-MHAC groups. Trace element deposition results also showed that tibial Mn increased (linear or quadratic, P = 0.002 and 0.009, respectively) in groups fed diets with increased levels of Mn-MHAC. In contrast, Fe deposition decreased both in the heart (linear, P = 0.020) and tibia (P < 0.05). In addition, the Mn-MHAC supplement noticeably lowered serum Mn-SOD activity (linear or quadratic, P = 0.048 and 0.019, respectively). The relative mRNA levels of divalent metal transporter 1 (DMT1) (P = 0.024), ferroportin 1 (FPN1) (P = 0.049), and Cu transporter-1(CTR1) (P < 0.001) in the duodenum, as well as CTR1 in the jejunum and ileum (P = 0.040 and 0.011, respectively) were higher in the Mn-supplemented group than in the control group. Furthermore, the relative mRNA level of DMT1 in the jejunum and ileum of broilers in the Mn-MHAC group (50 mg Mn/kg diet) did not differ from those in the control group, but was lower than those in the Mn sulfate group (P < 0.05). In conclusion, Mn-MHAC dietary supplementation improved the growth performance and trace element deposition in broilers. From this study, we recommend the optimum Mn-MHAC level to meet the Mn requirement of broilers is 50 to 75 mg Mn/kg diet.  相似文献   

3.
Pea starch consists predominantly of C-type of amylopectin chain which is more resistant to digestive enzymes than A-type of starch thus slowly digested in poultry. It was hypothesized that the presence of slowly digested pea starch in broiler diets will increase net energy and the efficiency of energy utilization in broilers. Two experiments were performed to investigate starch digestibility of pea at different incubation times (in vitro study) and the effect of dietary pea on heat increment and net energy in broilers using an open-circuit respiratory calorimetry system (in vivo study). One-day-old Ross 308 male broilers were fed a common starter crumble from d 1 to 10 and standard grower diets thereafter. At d 21, birds were transferred to the chambers each housing 2 birds. Each treatment was replicated 6 times with 2 identical runs of 3 replicates per treatment. A wheat-soybean meal-based diet was used as a control and the treatment diet contained 500 g of pea/kg pea. In vitro study showed that pellet processing increased (P < 0.001) starch digestibility, particularly at shorter times for wheat and a much larger response for pea. Birds offered the pea-based diet had lower (P = 0.002) feed intake, lower (P = 0.020) body weight gain, but a similar (P > 0.05) FCR compared to those offered the wheat-based diet. Net energy (NE) and apparent metabolizable energy (AME) values were higher in the pea-based diet than in the wheat-based diet (P = 0.037 for NE and P = 0.018 for AME). Heat production, respiratory quotient, heat increment of feed, efficiency of utilization of gross energy for AME, and efficiency of utilization of AME for NE did not differ (P > 0.05) between the 2 treatments. There was no effect (P > 0.05) of pea on the total tract digestibilities of dry matter, crude protein and ash, but the total tract digestibility of starch was higher (P = 0.022) in the pea-based diet compared to the wheat-based diet. This study provides insight into the energy metabolism of broilers offered a pea-based diet and indicates that dietary pea supplementation increases dietary AME and NE but has no effect on heat increment of feed and the efficiency of energy utilization in broilers.  相似文献   

4.
The aim of this study was to evaluate the effects of dietary betaine (BET) on growth performance, redox state, and related gene expression in broilers under heat stress (HS). A total of 144 21-day-old male broiler chickens with similar body weights were assigned randomly to three treatments with six replicates (eight chickens per replicate cage). Broilers in the control (CON) group were kept at thermoneutral (TN, 22±1°C) conditions and fed a basal diet until they were 42 days of age. Broilers in the other two groups (defined as HS and HS + BET) were exposed to HS (34±1°C, 8 h/day) and fed the basal diet without or with 1000 mg/kg BET, respectively. Rectal and cockscomb temperature of broilers was increased (P<0.05) in HS and HS + BET groups compared with the CON group, whereas there was no difference between HS and HS + BET groups. Dietary BET supplementation restored (P<0.05) average daily gain (ADG) and average daily feed intake (ADFI) of broilers and reversed (P<0.05) the increase in serum alanine transaminase (ALT) activity and malondialdehyde (MDA) content in the liver tissue of broilers under HS. The HS + BET group had higher (P<0.05) activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the liver tissue and mitochondria than the HS group, and the same pattern was observed for glutathione (GSH) and GSH/glutathione disulphide (GSSG) in the liver tissue. The decreased mRNA levels of GPX1 and uncoupling protein (UCP) in the liver induced by HS were restored by BET supplementation. In conclusion, dietary BET supplementation can alleviate HS-induced hepatic and mitochondrial oxidative damage of broilers by regulating mRNA expressions of GPX1 and UCP.  相似文献   

5.
The effects of dietary supplementation of zinc (Zn) sources and concentrations were investigated on growth performance, absorption into tissues, fecal excretion, nutrient retention, and intestinal morphology in broilers fed a corn-soybean meal basal diet. A total of 525 one-day-old chicks (Ross 308) were assigned based on body weight to seven dietary treatments. There were five replicate pens for each treatment and 15 broilers per replicate pen. The dietary treatments included a basal diet (control, without supplementing Zn), and basal diet supplemented with Zn, as inorganic zinc sulfate (ZnS; 110 mg/kg); organic Zn-methionine (ZnM; 110 mg/kg); hot-melt extruded (HME) 25 zinc sulfate (27.5 mg/kg); HME50 zinc sulfate (55 mg/kg); HME75 zinc sulfate (82.5 mg/kg); or HME100 zinc sulfate (110 mg/kg) for 35 days in two phases (d 1–21, phase I and d 22–35, phase II). Bodyweight and feed efficiency of broiler chicks fed diets supplemented with increasing dietary concentrations of HME-Zn improved linearly during the study period (P<0.05). Compared to the control treatment, the ZnS, ZnM, and HME diets increased Zn concentrations in the serum and liver. Inorganic ZnS supply resulted in the highest Zn concentration in excreta. Increasing supplemented Zn content in diets as HME linearly increased Zn concentration in the excreta, serum, liver, and tibia. Broiler chicks fed diets supplemented with increasing concentrations of HME increased villus height (VH; linear and quadratic) of the jejunum and VH of the ileum (linear). Increasing concentrations of dietary Zn supplied as HME resulted in linearly enhanced dry matter, gross energy, and nitrogen retention of broilers on day 21. These results suggest that dietary HME-Zn at a lower level (55 ppm) shows the same growth performance as common ZnSO4 at 110 ppm.  相似文献   

6.
The study was conducted to test the effects of using yeast culture (Saccharomyces cerevisiae) as feed additive on the growth performance, noxious gas emission, utilization of nutrients, excreta microbial count, and meat quality of broilers. In total, 360 one-day-old Ross 308 broilers with average body weight (BW) of 42.90±1.43 g were randomly selected and allotted to two groups; they were fed either a basal diet (control) or a basal diet supplemented with 1% yeast culture (YC). Each treatment group had 10 replication pens and each replication contained 18 birds. The experiment was divided into 3 phases (1 to 7, 8 to 21, and 22 to 35 days) for growth performance observation. In the 1st phase (1 to 7 days), only the body weight gain (BWG) significantly increased (P<0.05) in birds with the YC diet compared to the control diet. Significant effects on BWG (P<0.05) and feed conversion ratio (FCR) (P<0.05) were seen in birds receiving the YC-supplemented diet in the 3rd phase (22 to 35 days) as compared to the control diet. In addition, during the overall period (1–35 d), BWG was significantly higher (P<0.05) and FCR was reduced (P<0.05). Throughout this experiment, the meat quality, nutrient utilization, noxious gas emission, and bacterial count in the excreta did not vary significantly between the groups. This study proved that a higher dose of YC (Saccharomyces cerevisiae) supplementation could maintain the consistent positive effect on broiler growth but eliminated the speculated outcomes on digestibility, bacterial count, or excreta gas emission.  相似文献   

7.
1. The present experiment was designed to examine arginine (Arg)-sparing effects of guanidinoacetic acid (GAA) on production performance, intestinal morphology and certain blood parameters in broiler chickens.

2. A total of 300 male broiler chicks (Ross 308) were randomly allotted to 5 dietary treatments in a completely randomised design during 1–15 and 15–35 d rearing periods. Experimental treatments consisted of a basal diet with a reduction in Arg content (CON?; starter: 12.1 g/kg and grower: 11.3 g/kg Arg), a CON? diet supplemented with synthetic Arg equal to the required Arg level (CON+; starter: 13.80 g/kg; grower: 12.37 g/kg) and CON? diet supplemented with three levels of GAA (GAA0.6; GAA1.2; and GAA1.8; g/kg).

3. Supplemental GAA at 0.6 and 1.2 g/kg reduced the adverse effects of the CON? diet on weight gain and feed conversion ratio in the starter period, equal to the effect of CON+ diet. During the entire rearing period, such an effect was only seen for broilers fed CON? diet supplemented with 1.2 and 1.8 g/kg GAA (P < 0.05). Proportional weights of liver and abdominal fat were decreased in birds fed diets containing supplemental Arg and 1.8 g/kg GAA compared to the CON? broilers (P < 0.05). Jejunal villus height and villus height to crypt depth ratio were increased in broilers receiving the CON? diet (P < 0.05). Serum concentration of nitric oxide increased when the CON? diet was supplemented with either Arg or 1.8 g/kg GAA (P < 0.05).

4. Dietary inclusion of GAA had an Arg-sparing effect, whereby 1.2 and 1.8 g/kg of supplemental GAA resulted in greater growth performance during the starter and entire rearing periods, respectively. Supplementation with 1.8 g/kg GAA had significant effects on some carcass and physiological parameters.  相似文献   


8.
Beta-glucan has been shown to have a beneficial effect on gastrointestinal health. This experiment was conducted to investigate the effects of β-glucan isolated from Agrobacterium sp. ZX09 on growth performance and intestinal health of weaning pigs. A total of 108 weaned pigs (21 d of age; 6.05 ± 0.36 kg) were randomly divided into 3 groups (6 pens/group; 6 pigs/pen), and the groups were each treated with the following diets: 1) basal diet, 2) basal diet supplemented with 20 mg/kg olaquindox, 3) basal diet supplemented with 200 mg/kg β-glucan, for 21 d. Compared with the control group, pigs fed with 200 mg/kg β-glucan had greaterBW, average daily gain and duodenal villus height to crypt depth ratio (P < 0.05). Olaquindox increased the duodenal or jejunal villus height of pigs compared with β-glucan. Compared with the control group, β-glucan tended to increase the occludin mRNA expression in the jejunum (0.05 < P < 0.10). Beta-glucan enriched the beneficial microbiota in the ileum of pigs (P < 0.05). In conclusion, β-glucan may promote growth performance by improving intestinal health and increasing beneficial microbiota of weaned pigs. The study results will provide valuable theoretical guidance for the utilization of β-glucan in weaned pigs.  相似文献   

9.
The experimental design consisted of 5 dietary treatments including a positive control (PC), a negative control (NC; with a reduction of 88 kcal/kg of AME through the starter and grower 1 phase and a reduction of 132 kcal/kg of AME in the grower 2, finisher, and withdrawal phases compared with the PC), and the NC supplemented with either β-mannanase, nonstarch polysaccharide-degrading enzymes (NSPase; cocktail carbohydrase), or β-mannanase and NSPase intermittently fed. The intermittent treatment included β-mannanase from d 1 to 21 (starter and grower 1 phases) and NSPase from d 22 to 47. Each treatment included 9 replicate pens with 35 male broilers placed per replicate (1,575 total chicks placed). The dietary program consisted of 5 dietary phases, including the starter through d 10, grower 1 through d 21, grower 2 through d 32, finisher through d 40, and withdrawal through d 47. Broilers were weighed and feed consumption determined on days of dietary changes. On d 48, following an 8-h feed withdrawal, 6 broilers from each replicate pen were removed and processed for whole bird and fat pad measurements. The reduction in energy in the NC diet reduced BW and increased mortality rate, and the inclusion of β-mannanase and NSPase separately and intermittently in the NC diet improved growth performance and reduced mortality to levels that were comparable to the PC. The NC yielded the highest cumulative mortality-corrected FCR and all enzyme inclusion treatments reduced FCR to levels comparable to the PC for the duration of the trial. The NC diet yielded the lowest processing yields and the inclusion of β-mannanase and NSPase separately and intermittently increased multiple processing parameters to a level similar to the PC. These data confirm the ability of β-mannanase and NSPase inclusion separately and intermittently to improve performance parameters in reduced-energy broiler diets.  相似文献   

10.
The aim of this study was to determine the effect of dietary Forsythia suspensa extract (FSE) supplementation to lactating sows and nursery pigs on post-weaning performance, antioxidant capacity, immunoglobulins, and intestinal health. Based on backfat, body weight (BW), and parity, 24 gestating sows (Landrace × Yorkshire) with average parity of 3.38 ± 0.61 and BW of 234 ± 6.81 kg were allotted into two dietary treatments (control vs. 100 mg/kg FSE) with 12 sows per treatment from day 107 of gestation to day 21 of lactation. After weaning, based on the initial BW and source litter, 192 nursery pigs (Duroc × [Landrace × Yorkshire], average BW of 6.98 ± 0.32 kg, weaned at day 21) were allotted into four dietary treatments with eight replicate pens per treatment, six pigs per pen for a 4-wk study. The treatments included the following: 1) CC (sows and their piglets both fed control diet); 2) CF (sows fed control diet and their piglets fed FSE diet [containing 100 mg/kg FSE]); 3) FC (sows fed FSE diet and their piglets fed control diet); and 4) FF (sows and their piglets both fed FSE diet). The MIXED procedures of SAS for a split-plot arrangement with sow diet as the whole plot and nursery diet as split plot were used to analyze the data. After weaning, piglets from FSE-fed sows had improved (P < 0.05) average daily gain and feed efficiency, and lower (P < 0.05) diarrhea rate in overall (day 1 to 28) compared with those from sows fed control diet. Piglets from FSE-fed sows also had higher (P < 0.05) contents of immunoglobulin G (IgG), growth hormone, superoxide dismutase (SOD), total antioxidant capacity in serum, villus height in ileum, and villus height to crypt depth ratio in jejunum, as well as lower (P < 0.05) content of malondialdehyde (MDA) in serum and crypt depth in ileum compared with those from sows fed control diet. Piglets fed FSE during nursery had increased (P < 0.05) concentrations of IgG, SOD, and catalase, and decreased (P < 0.05) MDA and tumor nuclear factor-α levels in serum compared with those fed control diet during nursery. Piglets from FC group had increased (P < 0.05) protein expression of occludin in jejunal mucosa and relative abundance of Lactobacillus on genus level in colon compared with those from CC group. In conclusion, for the performance and intestinal health, diets supplemented with FSE during lactation phase seemed more efficient to alleviate weaning stress than the nursery phase. In terms of the antioxidant status and immunoglobulins, FSE supplemented in both phases were efficient for nursery pigs.  相似文献   

11.
A perennial schrub, stevia, and its extracts are used as a natural sweetener and have been shown to possess antimicrobial properties. Stevia contains high levels of sweetening glycosides including stevioside which is thought to possess antimicrobial and antifungal properties. Little is known about the nutritional value of the schrub in livestock. This study determined the potential use of the shrub as a prebiotic animal feed supplement in light of the recent ban on the use of antibiotics in animal feed and the role of its constituent stevioside in the effects of the shrub. Male Cobb broiler chicks were fed a basal broiler diet without antibiotic but with performance enhancing enzyme mix (positive control), a basal diet without antibiotic and enzymes (negative control), or diets in which 2% of the negative control diet was replaced with either dried ground stevia leaves or 130 ppm pure stevioside during 2 week starter and 2 week grower periods. Body weight gains, feed conversion, abdominal fat deposition, plasma hormone and metabolites and caecal short chain fatty acids (SCFA) were measured in the broilers at 2 and 4 weeks of age. There was no significant effect of the treatments on feed intake during the starter period but birds fed diet supplemented with stevia leaves and stevioside consumed more feed (p < 0.05) than those fed the positive control diet during the grower period. Weight gain by birds fed the positive control and stevioside diets was higher (p < 0.05) than those fed other diets only during the starter period. Feed/gain ratio of birds fed the positive control and stevioside diets was superior (p < 0.05) to others. There was no effect of the treatments on nutrient retention and water content of the excreta. Dietary stevia leave and stevioside decreased total concentration of SCFA and changed their profile in the ceca. There was no effect of the treatments on pancreas weight. Dietary stevia reduced blood levels of glucose, triglycerides and triiodothyronine (T3) but had no effect on non-esterified fatty acids. In contrast, stevioside only decreased T3. Both the stevia leaves and stevioside diets significantly increased abdominal fat content. It is concluded that dietary enzyme growth promoters are beneficial to the broilers only during the starter stage and that inclusion of stevia leaves or stevioside has no beneficial effect on the performance of broilers.  相似文献   

12.

Background

This study was designed to evaluate the efficacy of DL-selenomethionine (DL-SeMet) supplementation on growth performance, antioxidant status, plasma selenium (Se) concentration, and immune function of weaning pigs. 216 weaning pigs were randomly allocated to 6 treatments with 6 replicates each according to a complete randomized block design. Each replicate had six pigs. Diet of group one was corn-soybean basal diet without any additional Se supplement. Group 2 was supplemented with 0.3 mg/kg of Se from sodium selenite. Groups 3-6 were supplemented with 0.1, 0.3, 0.5, and 0.7 mg/kg of Se from DL-SeMet, respectively. The trial lasted for 42 days.

Results

Pigs supplemented with 0.3 and 0.7 mg/kg DL-SeMet obtained better feed gain ratio (P < 0.05). The best antioxidant ability (serum, liver, and muscle) was shown in 0.1-0.3 mg/kg DL-SeMet groups (P < 0.05). The plasma Se concentration increased as the dietary DL-SeMet level elevated. The immunity among groups was not affected.

Conclusions

DL-SeMet supplementation in the diet significantly improved the growth performance, antioxidant ability and plasma Se content of weaning pigs. DL-SeMet can replace sodium selenite in the diet of weaning pigs.  相似文献   

13.
This experiment was conducted to investigate the effects of antimicrobial peptide on growth performance and immune function of 817 broiler hybrids. Nine hundred one-day-old 817 broiler hybrids were chosen and randomly divided into 5 groups with 6 replicates per group and 30 broilers per replicate.The broilers in control group were fed with basal diet, broilers in the antibiotic group were fed with basal diet supplemented 150 mg/kg aureomycin,and that in groups Ⅰ,Ⅱ and Ⅲ were fed with basal diet supplemented 100,200 and 300 mg/kg antimicrobial peptide,respectively. The experiment period was 42 days. The results showed that average daily gain of 817 broiler hybrids in groups Ⅱ and Ⅲ was significantly increased (P<0.05),meanwhile the F/G was significantly decreased at 1-21 days old (P<0.05).The immune organ indexes of thymus,bursa of Fabricius and spleen were significantly increased at 21 and 42 days old (P<0.05),and the antibody level against ND and T lymphocyte transformation rate of 42 days old 817 broiler hybrids were improved (P<0.05). It indicated that antimicrobial peptide could increased growth performance and immune function of 817 broiler hybrids as same with aureomycin,and appropriate dosage of antimicrobial peptide was 200 mg/kg in this experiment.  相似文献   

14.
Three experiments (Exp. 1, n = 144 broilers [Ross × Ross]; Exp. 2, n = 118 mallard ducklings [Anas platyrhynchos]; and Exp. 3, n = 75 mature mallard ducks) were conducted to determine the effects of 3 levels of unmilled hybrid rice on growth performance and organ and gastrointestinal tract development. The dietary treatments were 1) corn-soybean meal (basal), 2) basal + 5% hybrid rice, and 3) basal + 10% hybrid rice for Exp. 1 to 3, respectively. One bird from each pen in Exp. 1 (n = 24) and all the birds in Exp. 2 (n = 118) and Exp. 3 (n = 75) were randomly selected and euthanized to determine linear measurements and organ and gastrointestinal tract weight. In Exp. 1 and 2, birds fed 10% rice experienced slower growth (P < 0.05) than birds fed the basal diet. In Exp. 3, the addition of rice did not affect growth performance. Rice addition did not affect organ length or weight (P > 0.05) in Exp. 1. However, birds fed 5% rice had significantly increased (P < 0.05) pancreas, ileum, and jejunum weights in Exp. 2, and 10% rice significantly increased (P < 0.05) liver weight in Exp. 3. The addition of 10% unmilled rice to broiler and duck diets may reduce growth performance.  相似文献   

15.
The present study was to evaluate the effects of dried Allium mongolicum Regel (AMR) powder and its water- and fat-soluble extracts (AWE and AFE) on the growth performance, serum metabolites, immune responses, antioxidant status, and meat quality of lambs. A total of 32 male small-tailed Han lambs (5 months old; initial body weight = 34.8 ± 0.40 kg) were used in a 60-d feeding experiment after a 15-d adaptation period. The lambs were randomly divided into 4 groups (n = 8) and fed a basal diet (control, CON group), the basal diet supplemented with dried AMR powder at 10 g/d per lamb (AMR group), the basal diet supplemented with AWE at 3.4 g/d per lamb (AWE group), or the basal diet supplemented with AFE at 2.8 g/d per lamb (AFE group). Blood samples were collected on d 0, 30, and 60 in the feeding experiment (n = 8). At the end of the experiment, the lambs were sacrificed and the longissimus dorsi muscles collected. Growth performance was not significantly affected by dietary supplementation of AMR, AWE and AFE (P > 0.05). However, significantly lower albumin (P = 0.006), total protein (P = 0.006), globin (P = 0.025), and blood urea nitrogen (P = 0.024) concentrations were observed in AFE group relative to CON and AMR groups. Similarly, a significantly lower lactate dehydrogenase activity (P = 0.018) was observed in AFE group relative to AWE group, but not in other groups (P > 0.05). In addition, significantly increasing trends in glutathione peroxidase (P = 0.06) in AMR, AWE, and AFE groups were observed relative to the control group. Furthermore, significantly lower drip loss (P = 0.011) across the treatment groups and cooking loss (P = 0.048) were observed in the AMR group relative to the control group. Taken together, these results indicate that AMR and its extracts had no significant effect on lamb growth performance, antioxidant status, and immune responses, but could significantly improve meat quality without the occurrence of pathological kidney and liver lesions.  相似文献   

16.
In an experiment with 2,040 Ross 308 broiler chickens, the effect of substituting inorganic with organic minerals in broiler feed on performance was determined. The experiment comprised 2 dietary treatments, with 6 replicates of 170 mixed-sex broiler chickens per pen replicate. Experimental diets consisted of a control treatment, formulated with inorganic Mn, Zn, Fe, and Cu sulfates at levels of 70, 37, 45, and 12 ppm, respectively, and an organic mineral diet supplemented with lower levels of Mn, Zn, Fe (all 10 ppm), and Cu (2.5 ppm) supplied as peptide chelates (Bioplex). Production performance was measured during the 39-d trial period, and mineral excretion was evaluated at 26 d of age. In the starter period (0 to 14 d), FCR tended to improve (P = 0.06) in broilers fed the organic mineral diet. However, no significant differences were observed in any of the productive performance parameters measured during the trial. Significantly lower (P < 0.05) excretion rates were recorded for all minerals in fecal samples taken from broilers receiving the organic mineral diet. Fecal levels of Mn, Zn, Fe, and Cu were 46, 63, 73, and 55%, respectively, compared to the controls.  相似文献   

17.
Prior research indicated that foot ash determinations were as robust as tibia bone ash determinations in reflecting the degree of bone mineralization in chicks at 14 d of age. In the current research, the relative effectiveness of the 2 procedures was evaluated in 21- and 42-day-old broilers while also evaluating a new dietary phytase supplement. In experiment 1, broilers were fed until 21 d of age a negative control diet with 0.24% available phosphorus, a positive control diet with 0.48% available phosphorus, or the negative control diet supplemented with 300, 500, 1,000, or 2,500 phytase units/kg diet. In experiment 2, broilers were fed until 42 d of age negative control diets having 0.275, 0.250, and 0.225 percent available dietary phosphorus in the starter, grower, and finisher periods, respectively, positive control diets having 0.475, 0.450, and 0.425 percent available dietary phosphorus in the starter, grower, and finisher periods, respectively, or the negative control diets supplemented with 500, 1,000, or 2,500 phytase units/kg diet. At 21 and 42 d of age, broilers fed diets supplemented with the 2 highest doses of phytase had foot and tibia ash values equal to those fed the positive control diet and higher than those fed the negative control diet. At 42 d of age, feed conversion and total breast meat yield values for the broilers fed the highest dose of phytase were superior to the values of the birds fed the positive control diet or the diet containing the lowest dose of phytase. The results indicate that adding levels of this new dietary phytase beyond what is necessary for normal bone mineralization enhances feed conversion and that dried foot and tibia bone ash determinations are both reliable in detecting differences in bone mineralization in 21- and 42-day-old broilers.  相似文献   

18.
A study was carried out to investigate the replacement effect of vitamin E with grape polyphenols (GPP) on antioxidant status, immune response, and organs histopathology in broilers from 1- to 35-d age. One-day-old Hubbard broiler chicks (n = 280) were randomly divided into 28 study units of 10 chicks each. A starter control diet was formulated having 22.5% CP, 3,200 kcal/kg ME, and 100 ppm vitamin E50 [50 IU DL-α-tocopheryl acetate (CON)]. Other dietary treatments were obtained by replacing vitamin E on wt:wt ratio, with 25 [low GPP (LGPP)], 50 [medium GPP (MGPP)], and 75 ppm [high GPP (HGPP)] GPP. Likewise, a control finisher diet (20% CP and 3,200 kcal/kg ME) and LGPP, MGPP, and HGPP diets were formulated having the same proportions of GPP and vitamin E as in the respective starter diets. Each dietary treatment was randomly assigned to 7 replicate pens. As a result of this study, by increasing GPP level in place of vitamin E, total phenolic contents in breast and leg muscles increased linearly (P< 0.05) whereas thiobarbituric acid reactive substances decreased quadratically with MGPP showing the lowest value (P< 0.05). Antibody titers against Newcastle disease virus were higher (P< 0.05) in broilers fed the LGPP and HGPP diets than in those fed the control diet. Antibody titers against infectious bursal disease virus were higher (P< 0.05) in broilers fed the HGPP diet than in all of the remaining dietary treatment groups. Histopathological examination of kidney and liver tissues showed no changes in birds fed LGPP and MGPP diets; however, mild effects were observed in some birds fed HGPP diet. Replacement of vitamin E with GPP resulted in lower cost per kg broiler feed. In conclusion, replacement of 75 ppm vitamin E with GPP improved antioxidant status and immune response of broilers at lower feed cost without any significant effect on histopathology of organs.  相似文献   

19.
The aim of present study was to evaluate whether diets supplemented with dihydroartemisinin (DHA) could alleviate intestinal inflammatory injury in weaned piglets with intrauterine growth retardation (IUGR). Twelve normal birth weight (NBW) piglets and 12 piglets with IUGR were fed a basal diet (NBW-CON and IUCR-CON groups), and another 12 piglets with IUGR were fed the basal diet supplemented with DHA at 80 mg/kg (IUGR-DHA group) from 21 to 49 d of age. At 49 d of age, 8 piglets with similar body weight in each group were sacrificed. The jejunal and ileal samples were collected for further analysis. The results showed that IUGR impaired intestinal morphology, increased intestinal inflammatory response, raised enterocyte apoptosis and reduced enterocyte proliferation and activated transmembrane toll-like receptor 4 (TLR4)/nucleotide-binding and oligomerization domain (NOD)/nuclear factor-κB (NF-κB) signaling pathway. Dihydroartemisinin inclusion ameliorated intestinal morphology, indicated by increased villus height, villus height-to-crypt depth ratio, villus surface area and decreased villus width of piglets with IUGR (P < 0.05). Compared with NBW piglets, IUGR piglets supplemented with DHA exhibited higher apoptosis index and caspase-3 expression, and lower proliferation index and proliferating cell nuclear antigen expression in the intestine (P < 0.05). Dihydroartemisinin supplementation attenuated the intestinal inflammation of piglets with IUGR, indicated by increased concentrations of intestinal inflammatory cytokines and lipopolysaccharides (P < 0.05). In addition, DHA supplementation down-regulated the related mRNA expressions of TLR4/NOD/NF-κB signaling pathway and upregulated mRNA expressions of negative regulators of TLR4 and NOD signaling pathway in the intestine of piglets with IUGR (P < 0.05). Piglets in the IUGR-DHA group showed lower protein expressions of TLR4, phosphorylated NF-κB (pNF-κB) inhibitor α, nuclear pNF-κB, and higher protein expression of cytoplasmic pNF-κB in the intestine than those in the IUGR-CON group (P < 0.05). In conclusion, DHA supplementation could improve intestinal morphology, regulate enterocyte proliferation and apoptosis, and alleviate intestinal inflammation through TLR4/NOD/NF-κB signaling pathway in weaned piglets with IUGR.  相似文献   

20.
Abstract

An experiment was conducted using 720 one-day-old Arbor Acres broiler chickens to evaluate the effects of Astragalus membranaceus root powder (AMP), Astragalus polysaccharides (APS) on growth performance, carcass characteristics, and antioxidant enzymes, and metabolites of blood and liver in broiler chickens. The six dietary treatments included a control that was a corn–soybean meal basal diet; basal diet supplemented with 120 mg/kg antibiotic; 350 mg/kg APS; or 5000, 10,000, or 15,000 mg/kg diet of AMP. Supplementation of 10,000 mg/kg AMP increased average daily gain and feed conversion rate of broilers in grower period (22–42 d) and over the entire experiment compared to basic diet. Responses of antioxidant status to dietary APS and AMP varied depending on the age of the broilers. Beneficial effects of AMP on growth and feed utilization of broilers could be obtained by inclusion of AMP at the rate of 10,000 mg/kg diet.  相似文献   

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