共查询到20条相似文献，搜索用时 713 毫秒
1.
2.
抗弓形虫和新孢子虫药物体外筛选方法的研究进展 《畜牧与饲料科学》2023,44(1):122-128
弓形虫和新孢子虫是2种在形态结构及生物学特征方面非常相似的细胞内寄生性原虫,二者引起的疾病对人和动物健康造成了严重危害。开发和筛选高效、低毒的抗弓形虫和新孢子虫药物一直是抗原虫药物研究领域的重要方向之一。药物筛选包括体外筛选和体内筛选,体外筛选的结果是进行体内筛选的依据和基础。对抗弓形虫和新孢子虫药物的体外筛选方法研究进展进行综述,以期为开发更加高效的抗弓形虫和新孢子虫药物体外筛选技术提供参考。 相似文献
3.
4.
隐孢子虫病检测技术研究进展 总被引：2，自引：1，他引：1
隐孢子虫是动物和人腹泻的重要病原,也是人类艾滋病患者的主要致死因素之一.当前,对隐孢子虫病的研究已成为全球寄生虫学研究领域的热点由,于迄今尚无治疗隐孢子虫病感染的特效药物,因此,快速、简便、准确地发现隐孢子虫是控制该病的关键.随着分子生物学技术的应用,隐孢子虫病的检测技术得到了极大的发展.笔者就近年来隐孢子虫病检测技术进行了简要综述. 相似文献
5.
6.
硝唑尼特抗免疫抑制小鼠隐孢子虫活性试验 总被引：1，自引：0，他引：1
研究硝唑尼特抗隐孢子虫的活性,采用地塞米松抑制昆明鼠免疫功能,经口接种感染微小隐孢子虫孢子化卵囊,成功制备出微小隐孢子虫感染的动物模型。将感染微小隐孢子虫的小鼠随机分为感染对照组以及高、中、低剂量3个药物剂量组,连续经口灌服硝唑尼特治疗1周,观察每只小鼠每天排出微小隐孢子虫卵囊的数量,以感染抑制率来评价硝唑尼特抗微小隐孢子虫的活性。结果表明,200 mg/(kg.d)剂量组能显著减少小鼠排出隐孢子虫卵囊的数量,对治疗微小隐孢子虫感染具有较好的疗效。 相似文献
7.
分子生物学技术在隐孢子虫研究中的应用 总被引：3，自引：0，他引：3
隐孢子虫是顶复门原虫，主要寄生于人或动物的消化道上皮并引起腹泻，是寄生虫学、环境微生物学研究热点之一。分子生物学技术给隐孢子虫研究注入了强大动力，本文就其在隐孢子虫检测、分子流行病学调查、分类学以及药物设计等研究领域的应用作一综述。 相似文献
8.
本文综述了国内外关于水牛住肉孢子虫病的病原、生活史、流行病学、临床与病理学、免疫诊断、肉品卫生及防治等方面的研究成果。详细论述了新证实的水牛枯氏住肉孢子虫及另外两种住肉孢子虫的形态学、发育史、致病性等生物学特性和水牛住肉孢子虫病的临床与病理学,五种检测抗原和抗体的免疫学诊断技术与药物防治方法。 相似文献
9.
奶牛贝诺孢子虫病(下称贝病)是由贝氏贝诺孢子虫(Besnoitia besnoiti)引起的一种原虫病,目前,国内外尚无有效的治疗药物。为此,我们应用多种药物进行治疗试验并选出了几种适于现地应用的、初见成效的药物,现报告如下。 相似文献
10.
住肉孢子虫系二宿主寄生物，其所致病性与防治工作同发育史密切相关。近些年来，国外许多学者依据虫体发育史特点，应用免疫、药物平方法在小范围内或通过实验感染进行了防治试验研究，获得了不同程度的效果；有的观察和介绍了温度对中间宿主胴体内住肉孢子虫存活期的影响等。国内仅有报道人作为终末宿主的肠壁型肉孢子虫病的防治试验。家畜住肉孢子虫病的药物防治试验尚未见有报道。作者仅就天然感染和人工感染的绵羊住肉孢子虫病病例作动物模型，进行了药特防治试验。 相似文献
11.
草鱼与青鱼、鳙鱼与团头鲂之间的细胞核移植 总被引：1，自引：0，他引：1
在草鱼与青鱼、鳊鱼与团头鲂之间进行核移植试验,获得了不同属鱼类间的草鱼细胞核和青鱼细胞质配合的核质杂种鱼——草青移核鱼幼鱼,以及不同亚科间的鳙鱼细胞核与团头鲂细胞质配合的核质杂种鱼——鳙团移核鱼,得率分别为0.9％和1.8％。核质杂种鱼的胚胎发育特征大多与受核体鱼相似,而形态特征大多与供核体鱼相似。表明胚胎发育明显受受核体细胞质的影响,而形态性状特征主要受供核体细胞核的影响。 相似文献
12.
Since 1985, 5 cases of feline conjunctival melanoma have been reported in the literature. Information on feline conjunctival melanoma epidemiological features, localizations, macroscopic features and histological features is limited. We are describing the clinical, histopathologic features and outcomes in a cat that presented clinically with a slow developing dark brown mass located under the upper eyelid of the left eye. Pertinent literature is reviewed; and the recognizable clinical features and treatment are discussed. The mass was surgically resected. Despite its size, the lesion was easily separated from underlying tissues, making possible a macroscopic complete resection that left intact the adjacent conjunctiva. The tumour histological examination has showed a pigmented melanoma lacking encapsulation, but presenting a clear zone delimiting the lesion. It was exclusively composed of epithelioid cells, and presented mild cellular anaplasia and weak mitotic activity. These features allowed it to be classified as a quite differentiated melanoma with few signs of potential malignancy. In accordance with these histologic features, no recurrence has been registered 34 months after surgery. Thus, a favorable outcome is now reported for two out of six cases of conjunctival melanoma in the cat. This report also confirms the predilection for this neoplasm to arise from the bulbar conjunctiva. 相似文献
13.
Patricia C Schultheiss 《Journal of veterinary diagnostic investigation》2006,18(4):422-425
Outcomes, signalments, and the relationship of histologic features with the outcome of melanomas located in lip, nail bed, and haired skin of dogs were reviewed. These melanomas were diagnosed as benign or malignant based on histologic features. Melanomas of the lip arose from mucous membrane in most cases. 32 dogs with lip melanomas that had histologic features of malignancy, 22 died because of the tumor within 1 year and 10 were tumor free for at least 1 year following removal. Of 10 dogs with melanomas with benign histologic features on the mucous membrane of the lip, 9 were tumor free for at least 1 year. Of 4 dogs with benign appearing tumors of the haired skin of the lip, 3 were tumor free for at least 1 year. Melanomas with histologic features of malignancy occurred in many locations of haired skin, and 11 of 24 dogs were tumor free for at least 1 year. All nail bed melanomas had histologic features of malignancy and all invaded the third phalanx, but 6 of 14 dogs were tumor free for at least 1 year after amputation of the digit. Among these dogs, the 1-year survival rates for tumors classified as malignant by histologic features were 69% for lip, 46% for haired skin, and 43% for nail bed. However, the clinical outcome of an individual malignant tumor could not be predicted accurately by any specific histologic features. 相似文献
14.
The sow provides a specific environment to her offspring during gestation and lactation. Certain features in the early life of the sow (sow history features) may affect her ability to deliver and feed a healthy litter. In genetic analyses of grow-finish traits, these effects are estimated as common litter or permanent sow effects. The objective of this research was to identify sow history features that affect the growth rate (GR) and feed intake (FI) of her offspring during the grow-finish stage. Data from 17,743 grow-finish pigs, coming from 604 sires and 681 crossbred sows, were recorded between May 2001 and February 2010 at the experimental farm of the Institute for Pig Genetics (Beilen, the Netherlands). The grow-finish stage was divided into 2 phases (phase 1: 26 to 75 kg; phase 2: 75 to 115 kg). The sow history features were birth litter size, birth year and season, birth farm, weaning age, age of transfer to the experimental farm, and age at first insemination. The sow features were added to the basic model one at a time to study their effect on the grow-finish traits of the pigs. Subsequently, significant sow features (P < 0.1) were fitted simultaneously in an animal model. With every extra piglet in the birth litter of the sow, the GR of her offspring decreased by 1 g/d and the FI decreased by 4 g/d. Every extra day to the first insemination increased the GR of grow-finish pigs by 0.1 g/d. The heritability estimates for GR and FI (only in phase 2 of the grow-finish stage) decreased after adding the sow features to the model. No differences were found in estimates of the common litter effects between the basic model and the model with all significant sow features. The estimates of the permanent sow effect changed for FI from 0.03 (basic model) to 0.00 (model with sow features), and for FI in phase 1, the permanent sow effect decreased from 0.03 (basic model) to 0.01 (model with sow features). In conclusion, selected sow features do affect the grow-finish traits of the pigs, but their estimates are small and explain only a small proportion of the differences in the GR and FI of grow-finish pigs. The sow features partially explained the permanent sow effect of FI-related traits and did not explain the common litter effect. Although the sow early life features can affect piglet traits, they do not predict which sows produce better performing offspring in the grow-finish stage. 相似文献
15.
Disease features in horses with induced equine monocytic ehrlichiosis (Potomac horse fever) 总被引：10，自引：0，他引：10
S K Dutta B E Penney A C Myrup M G Robl R M Rice 《American journal of veterinary research》1988,49(10):1747-1751
Fifty-five horses were inoculated IV and/or SC with materials containing Ehrlichia risticii, ie, infected whole blood, buffy coat cells, or cell culture, to study clinical and hematologic features of equine monocytic ehrlichiosis (Potomac horse fever). Major clinical and hematologic features of induced E risticii infection were biphasic increase in rectal temperature with peak increases of 38.9 C and 39.3 C on postinoculation days (PID) 5 and 12, respectively; depression; anorexia; decreased WBC count (maximal decrease of 47% on PID 12); and diarrhea from PID 14 to PID 18. Increased WBC count was an inconsistent feature, with a maximal increase of 51.5% on PID 20. During times of decreased and increased WBC counts, lymphocyte/neutrophil ratios remained fairly constant. However, not all horses had all clinical and hematologic features, and these features were present in different degrees among horses. Increased rectal temperature, depression, anorexia, and decreased WBC count were more consistent features, whereas diarrhea developed in 73% of the horses. Of 55 horses, 39 (71%) had all clinical and hematologic features of the disease (classic disease), whereas 16 (29%) horses did not have greater than or equal to 1 of these features (nonclassic disease). The E risticii titer in the blood (ehrlichemia) was maximum during the peak increase in rectal temperature. In 55 horses, mortality was 9%. Significant differences (P greater than 0.5) in clinical and hematologic features were not detected between horses that survived and those that died of E risticii infection. 相似文献
16.
中国地方猪种遗传资源系统保存策略与方案 总被引：1，自引：0，他引：1
针对中国地方猪种遗传资源保存要以活体保种为主要形式,且面临经济特性选育提高,以及品种数量多的三大特点,为突破以Wright有效含量为核心的保种理论关于避免选择等条件的限制,本文提出了变无目标的随机保种为有目标保种,变避免选择的静态保种为利用选择的动态保种,变各品种互不联系的孤立保种为特性统一分配的系统保种策略,并通过特性系统分配多目标规划数学模型,给出了中国猪种质特性的保存方案。该模型有两个目标,一是使特性安排在表现最突出的品种中保存,二是使品种选择特性之间遗传正相关大,负相关小。 相似文献
17.
假俭草的形态特征及其草坪质量评价 总被引：8，自引：1，他引：7
结合植物生态学与生物统计学的方法,从叶和茎两个方面研究了假俭草的外部形态特征,并对其草坪质量进行了综合评价。结果表明,叶片的形态特征指标基本符合草坪草的要求;假俭草的匍匐茎和直立茎在形态上存在较大差异;假俭草草坪具有优良的景观质量,适合建植各种类型的草坪,尤其适合建植保土草坪。 相似文献
18.
菊苣种质资源的重要形态性状的变异研究 总被引：3，自引：1，他引：2
为揭示不同菊苣(Cichorium intybus L.)种质形态学特征多样性和变异程度,确定优良菊苣种质的表型特点,对菊苣种质资源的14个重要形态学特征进行变异性研究。结果表明:不同菊苣种质资源的形态学特征存在广泛的变异,其中,三级生殖枝数变异幅度最大,为22.25~430.00个,变异系数高达55.64%;其次为花序数量和叶宽,两者变异系数分别为54.85%和45.44%;菊苣形态学特征间存在显著相关性,叶片窄长类型的菊苣种质,株高和生殖枝高度高,生殖枝数多,茎秆粗壮,花序数量多,种子细长且饱满,千粒重较大,结实性好,反之亦然。聚类分析表明,不同菊苣种质资源可划分为2大类,源自不同地区的菊苣材料,形态相似的材料基本聚为一类,与2个对照品种聚为一类的菊苣基本表现为侧根发达,花序数量繁多,植株较高,叶片长而宽,茎秆粗壮,花序长而宽,种子短而宽。 相似文献
19.
Hiraoka H Hisasue M Nagashima N Miyama T Tanimoto T Watanabe M Itamoto K Mizuno T Inokuma H Okuda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):665-668
An eleven-year-old female pug was referred to Yamaguchi University Animal Hospital for evaluation of anemia and thrombocytopenia. The cytological examination of the peripheral blood showed some giant monocytic lineage blast cells. A few granulocytes and platelets had dysplastic features. On day 7, in addition to increasing the monocytic lineage cells, the dysplastic features of the blood had also increased compared to the initial examination. We performed bone marrow aspiration upon her death. The bone marrow revealed dysplastic features in all three hematopoietic cell lines, and an increase in the monocytic cell line. Based on the features of the bone marrow and the peripheral blood, this case was confirmed to be myelodysplastic syndrome--Chronic myelomonocytic leukaemia (MDS-CMML). 相似文献
20.
The microscopic anatomy of the ringed seal lung exhibits unique features and many features similar to those described in other seal species. Unique features include: Trachealis muscle predominately oriented longitudinally; Large veins within the tracheal wall supported by elastic fibers; Goblet cells and pseudostratified epithelium lining the duct system of bronchial glands of the segmental bronchi; Lamina propria of the segmental bronchus heavily invested with elastic fibers clustered into dense longitudinal bundles; and Capillaries and venules covered with squamous epithelium protruding into bronchiolar lumina. Common features include: Cartilage support of the bronchial tree extending distally into respiratory bronchioles; Smooth muscle enhancements in the distal airways producing sphincter like formations; and Lungs extensively supported with interstitial tissue, which divide lungs into lobules. 相似文献