共查询到20条相似文献，搜索用时 300 毫秒
1.
本试验采用兔病毒性出血症灭活疫苗、兔病毒性出血症灭活疫苗和兔病毒性出血症病毒强毒两种不同的免疫程序来免疫家兔．采用血凝及血凝抑制试验检测血清的抗体效价。结果表明：经3次免疫后,疫苗免疫程序组免疫家兔的抗血清平均效价为9log2,未免疫家兔的抗体平均效价为2log2。 相似文献
2.
兔病毒性出血症（RHD）是由兔病毒性出血症病毒引起的一种急性、接触性、败血性传染病，其发病率和死亡率都很高，是兔的烈性传染病之一。该病至今尚无特效的治疗药物和方法，在养殖生产中主要依靠接种脏器组织灭活疫苗进行预防，保护效果可靠。然而笔者在近几年的兔病临床诊断中，发现免疫后兔病毒性出血症病例时有发生，探析免疫失败原因主要有以下几个方面。 相似文献
3.
兔病毒性出血症由兔病毒性出血症病毒引起，以长毛兔最易感染，其一年四季均可发生。2月龄以上的青年兔和成年兔的发病率和死亡率最高（可达95％以上），断奶幼兔对该病毒有一定抵抗力，哺乳期仔兔基本不发病。病毒可通过呼吸道、消化道、皮肤等多种途径引起感染，其潜伏期48～72h。 相似文献
4.
5.
酶联免疫吸附试验及免疫印迹技术检测兔血液滤纸片中兔出血症病毒抗体宋秉生（中国农业科学院中兽医研究所，兰州７３００５０）我国学者于１９８４年首次发现兔病毒性出血症［１］，随后很多国家发现了本病，其致死率很高。自发现本病以来，一直采用血凝抑制试验检测血清... 相似文献
6.
兔病毒性出血症研究概况 总被引：2，自引：0，他引：2
朵红 《青海畜牧兽医杂志》2005,35(3):41-42
本文对兔病毒性出血症病毒的形态及理化特性、兔病毒性出血症病毒的分子生物学、兔病毒性出血症病毒的诊断、兔病毒性出血症病毒的疫苗等方面进行了简要综述。 相似文献
7.
8.
9.
兔病毒性出血症(R H D)是由兔病毒性出血症病毒引起的一种急性、接触性、败血性传染病,其发病率和死亡率都很高,是兔的烈性传染病之一。该病至今尚无特效的治疗药物和方法,在养殖生产中主要依靠接种脏器组织灭活疫苗进行预防,保护效果可靠。然而笔者在近几年的兔病临床诊断中,发现免疫后兔病毒性出血症病例时有发生,探析免疫失败原因主要有以下几个方面。1不按免疫程序进行接种免疫接种是预防该病的有效手段,而良好的免疫程序是有效防止该病发生的关键。笔者发现发生兔病毒性出血症病例的养殖户在免疫接种时存在以下几种情况。11免疫接种… 相似文献
10.
汤少伟 《上海畜牧兽医通讯》2011,(6):76-77
兔病毒性出血症俗称兔瘟,是由兔病毒性出血症病毒引起家兔一种急性、烈性、高度致死性传染病。该病发病率、死亡率极高,一旦暴发,会给兔场带来毁灭性的打击。目前兔病毒性出血症仍以疫苗预防为主,但是养殖户发现,即使免疫注射过疫苗仍有发病死亡的情况发生。笔者根据多年的理论知识积累及实践工作经验,并结合当前养殖实际情况,对兔病毒性出血症免疫失败的原因作一总结,以期对广大养殖户有所帮助。 相似文献
11.
Chen SY Chou CC Liu CI Shien JH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(9):951-958
Rabbit hemorrhagic disease virus (RHDV) induced viral fulminant hepatitis in adult rabbits. We investigated the damage of renal function and electrolyte balance in experimentally infected rabbit by measuring the related serum parameters to elucidate the pathogenesis of RHDV as an index for medical treatment. Nineteen New Zealand White rabbits, ten females and nine males, were each intramuscularly inoculated with 0.5 ml 50% rabbit lethal dose (RLD(50)) rabbit hemorrhagic disease virus. Blood samples were collected at 0 hr post inoculation (HPI) and every 6 hr from 18 HPI repeatedly through 66 HPI. After virus inoculation, serum blood urea nitrogen (BUN), creatinine (CREA) and sodium (Na(+)) were elevated to a highly significant level (p<0.0001), whereas serum potassium (K(+)) was moderately elevated to a significant level (p<0.05). Hypoglycemia developed highly significantly (p<0.0001). Serum chloride ion (Cl(-)) was the only parameter which did not change significantly (p=0.077). No significant sexual difference was observed among these parameters. Renal insufficiency progressed from 36 hr, as indicated by the increases in BUN and CREA; significant changes in electrolytes resulting in the increased osmolality of extracellular fluid that induced flow disturbance which consequently destroy the homeostasis in cells. Therefore, the later impairments in renal function and electrolyte balance might be an important threat for rabbits which might have survived from acute fulminant hepatitis in RHD. 相似文献
12.
Teixeira L Marques RM Aguas AP Ferreira PG 《Veterinary immunology and immunopathology》2012,148(3-4):343-347
Rabbit hemorrhagic disease virus (RHDV) is the etiologic agent of rabbit hemorrhagic disease (RHD), an acute lethal infection that kills 90% of adult rabbits due to severe acute liver inflammation. Interestingly, young rabbits are naturally resistant to RHDV infection. Here, we have compared naturally occurring CD4(+)Foxp3(+) regulatory T cells (Tregs) between young and adult rabbits after infection by RHDV. The number and frequency of Tregs was decreased in the spleen of adult rabbits 24h after the RHDV infection; this was in contrast with the unchanged number and frequency of splenic Tregs found in young rabbits after the same infection. Also, serum levels of IL-10 and TGF-β were enhanced in the infected adult rabbits whereas no alteration was observed in infected young rabbits. However, this increase is accompanied by a burst of pro-inflammatory cytokines, but seems not able to prevent the death of the animals with severe acute liver inflammation in few days after infection. Since Tregs downregulate inflammation, we conclude that their decrease may contribute to the natural susceptibility of adult rabbits to RHDV infection. 相似文献
13.
14.
利用抗兔出血症病毒(RHDV)的特异性单克隆抗体(McAb)建立了ELISA阻断试验用以检测血清抗体,并与血凝抑制(HI)和琼脂扩散试验(ID)进行了比较。结果ELISA阻断试验敏感性超过HI,更远胜于ID,具有灵敏、特异、准确等优点,适于大批血清样品的快速检测。 相似文献
15.
16.
新型兔出血症病毒(RHDV2)在全球范围流行并已在我国出现,给养兔业带来巨大威胁。为了解RHDV2与经典兔出血症病毒(RHDV)的异同,从病原学、流行病学、临床症状、致病机理、诊断与防控等方面,阐述了两种病原的不同及相关研究的最新进展。对比发现:RHDV2与经典RHDV亲缘关系较远,抗原表位也存在明显差异,属于不同的病毒分支;经典RHDV主要感染成年家兔,而RHDV2对不同品种和年龄的兔均易感;经典RHDV感染常表现为急性症状,而新型RHDV感染以亚急性和慢性病症为主。目前针对RHDV2的检测和疫苗研究还相对滞后。鉴于RHDV2与经典RHDV之间免疫交叉性较弱,免疫预防上存在较大空白,下一步应加大RHDV2毒株结构功能研究,加快疫苗开发,做好可疑病例处置和流行病学研究,及时掌握我国疫情动态。本文为我国兔病毒性出血症的预防与控制提供了参考。 相似文献
17.
18.
19.
20.
Wang X Qiu L Hao H Zhang W Fu X Zhang H He S Zhang S Du E Yang Z 《Veterinary immunology and immunopathology》2012,145(1-2):277-282
Vaccine antigens for rabbit hemorrhagic disease virus (RHDV) are currently derived from inactivated RHDV obtained from the livers of experimentally infected rabbits or from several recombinant immunogens. However, the application of these vaccine antigens has been restricted because of biosecurity and immunity characteristics. In the current study, a recombinant adenovirus expressing the RHDV capsid protein (VP60) was constructed and the expression of the recombinant protein was identified through western blot analysis using RHDV-positive rabbit sera. Eighteen rabbits were immunized by injection, direct oral instillation, or using bait. They were challenged with RHDV isolate three weeks after boost immunization. In all cases, the rabbits immunized with the recombinant adenovirus developed RHDV-specific antibodies and cell immune response. The rabbits injected with the recombinant adenovirus were completely protected against RHDV challenge. The adenovirus expression system may provide a strategy for the immunization of rabbits, particularly for the control of RHDV in wild rabbits. 相似文献