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1.
1.?The study was conducted to determine the effects of dietary supplementation of enzyme and oregano essential oil at two levels, alone or together, on performance, digestive enzyme, nutrient digestibility, lipid metabolism and immune response of broilers fed on wheat–soybean meal based diets.

2.?The following dietary treatments were used from d 0 to 21. Diet 1 (control, CONT): a commercial diet containing no enzyme or oregano essential oil, diet 2 (ENZY): supplemented with enzyme, diet 3 (EO250): supplemented with essential oil at 250 mg/kg feed, diet 4 (EO500): supplemented with essential oil at 500 mg/kg feed, diet 5 (ENZY + EO250): supplemented with enzyme and essential oil at 250 mg/kg, and diet 6 (ENZY + EO500): supplemented with enzyme and essential oil at 500 mg/kg.

3.?Birds fed on diets containing ENZY, EO250 and ENZY + EO250 had significantly higher weight gain than those given CONT diet from d 0 to 7. No significant effects on feed intake, feed conversion ratio, mortality, organ weights except for jejunum weight and intestinal lengths was found with either enzyme or essential oil, alone or in combination, over the 21-d growth period. The supplementation of essential oil together with enzyme decreased jejunum weight compared with essential oil alone.

4.?Supplementation with enzyme significantly decreased viscosity and increased dry matter of digesta, but did not alter pH of digesta. There was no effect of essential oil alone at either concentration on viscosity, dry matter or pH of digesta. A significant decrease in viscosity of digesta appeared when essential oil was used with together enzyme.

5.?The supplementation of essential oil at both levels with or without enzyme significantly increased chymotrypsin activity in the digestive system, and improved crude protein digestibility.

6.?The higher concentration of essential oil with and without enzyme significantly increased serum total cholesterol concentrations. No significant effect on immune response was found with either enzyme or essential oil, alone or together.

7.?Enzymes and essential oil had different modes of actions. The supplementation of enzyme with essential oil in diets is likely more effective in view of performance, nutrient digestibility, enzyme activities and immune system.  相似文献   


2.
1. Endo-1,4-β-xylanase is known to improve the nutritive value of wheat-based diets for poultry by degrading dietary arabinoxylans. However, broilers’ response to supplementation of wheat-based diets with exogenous endo-1,4-β-xylanase is not always observed.

2. In this study, 108 different wheat lots were analysed for levels of extract viscosity as well as for endogenous endo-1,4-β-xylanase activity, and the impact of these two variables in animal performance was tested.

3. Results revealed that endogenous endo-1,4-β-xylanase activity and extract viscosity content varied widely among different wheat lots. Thus, a trial was conducted to evaluate the efficacy of exogenous enzyme supplementation in broiler diets using wheats with different levels of extract viscosity and endogenous endo-1,4-β-xylanase activity.

4. The data revealed that exogenous enzyme supplementation was only effective when the wheat present in the diet had high levels of extract viscosity (14.8 cP) with low endogenous endo-1,4-β-xylanase activity (347.0 U/kg). Nevertheless, it is apparent that exogenous microbial xylanases reduce digesta extract viscosity and feed conversion ratio independently of the endogenous properties presented by different wheat lots.

5. The data suggest that extract viscosity and/or endogenous endo-1,4-β-xylanase activity affect the response to enzyme supplementation by poultry fed on wheat-based diets.  相似文献   


3.
1. The influences of genotype, age and sex on droppings digestibility coefficients of a compound food were studied using male and female broiler chickens of three different genotypes at 2, 4 and 6 weeks of age.

2. Because the traditional method of determination of droppings digestibility coefficients of nitrogen may lead to systematic errors in estimating the feeding value of foodstuffs, a method is proposed to determine the ileal digestibility coefficients. The ileal method is compared with the droppings method for a mixed food and for two foodstuffs: wheat and solvent‐extracted soyabean meal.

3. Birds selected for efficient food conversion showed distinctly higher digestibility coefficients for all nutrients than birds selected for high growth potential or birds from a commercial strain.

4. The influence of age on digestibility coefficients was not consistent.

5. Female birds showed digestibility coefficients which were, in general, 3% higher than those of male chickens.

6. Interactions between genotype and sex and between genotype and age for energy metabolisability were the only interactions observed for digestibility measurements.

7. The method of determination influenced the amino acid digestibility coefficients of the mixed food and the relative feeding values of wheat and soyabean meal.

8. It is important to use well defined animals (genotype, sex, age) in evaluating foodstuffs.

9. The preferred method for determination of digestibility coefficients of nitrogen and amino acids is based on ileal sampling, although the differences in amino acid digestibility coefficients were small between methods.  相似文献   


4.
1. Japanese quail eggs from moderately heavier sires showed superior fertility; while fertile eggs from moderately heavier dams hatched slightly better than the eggs from lighter dams.

2. Higher rates of fertility and hatchability of Japanese quail eggs were observed from parents of 10 to 19 weeks of age, with peak fertility and hatchability at 14 and 12 weeks of age, respectively.

3. Sex ratios of 1:2 to 1:5 gave comparable fertility and hatchability results.

4. The hatching performance of quail eggs from cage and deep litter reared breeders was comparable.

5. Fertility and hatchability were directly proportional to the egg weight.

6. Quail egg shell colour, tints and blotches were found to influence hatching performance.

7. Storing quail eggs at 16 ± 2°C and 75 ± 5% relative humidity for more than 4 d reduced hatchability.

8. Hatchability of eggs stored at room temperature was improved if they were sealed in polyethylene bags.

9. Provision of light during the first 14 d of incubation resulted in a photo‐acceleration of about 3.2 h.  相似文献   


5.
1. It has been shown that both the variety of wheat and the environment under which growth took place influenced the quantities of starch and cell wall carbohydrate contents of wheat.

2. The different varieties of wheat investigated contained different proportions of water‐soluble to water‐insoluble hemicelluloses.

3. By comparison of the arabinose to xylose ratios it was concluded that water‐soluble and insoluble arabinoxylans of wheat were branched to the same degree.

4. Starch from six wheat varieties grown in the U.K. was very well digested by adult cockerels and true metabolisable energy values of the wheats were high.

5. Small amounts of cell wall carbohydrates were digested by adult cockerels.  相似文献   


6.
1. Chicks of a light‐bodied strain were deprived of food on alternate days from 15 to 46 d of age.

2. Hyperphagia, amounting to twice the intake of control birds early in the experiment, occurred on the repletion day.

3. Final body weight attained 80% of that of the controls.

4. Body fat concentration decreased at first but returned to that of controls after 3 weeks.

5. A consistent hyperlipaemia was observed after food deprivation or restoration.

6. There was a marked increase in the digestive enzymes found in the small intestine, but not in the pancreas during the repletion day.

7. These responses may be considered as mechanisms to reduce the physiological manifestations of starvation.  相似文献   


7.
1. The study aimed to establish the optimum time after ingestion and optimum sampling site for the development of an ileal amino acid digestibility assay for broiler chickens.

2. To establish the optimal sampling time, 4‐week‐old broiler chickens were given one of 6 protein sources (meat‐and‐bone, soyabean, cottonseed, fish, maize and wheat meals) as the sole source of protein in a test diet. The diets contained chromic oxide as an indigestible marker. The birds were starved for 24 h, fed and subsequently killed for sampling of ileal digesta (terminal 15 cm) at 2, 3, 4, 5 and 6 h after the start of feeding.

3. For the soyabean, fish, wheat and maize meal diets, sampling time had no significant effect on apparent ileal nitrogen digestibility, whereas for the meat‐and‐bone and cottonseed meal diets there was a significant quadratic effect of sampling time. The amount of digesta collected was maximised and the mean apparent ileal nitrogen digestibility had the lowest variation around the 4 h sampling time.

4. To establish the optimum sampling site, 4‐week‐old chickens were given either a meat‐and‐bone, a soyabean or a wheat bran meal‐based diet. The birds were killed 4 h after the start of feeding, and digesta were sampled from 0.10, 0.15, 0.20 or 0.25 cm of terminal ileum.

5. There was no significant effect of sampling site on the apparent ileal digestibility of dietary nitrogen. The terminal 15 cm of ileum was considered a preferred site for sampling ileal digesta from broiler chickens.  相似文献   


8.
1. Genetically fat and lean adult broiler breeder females were fed ad libitum or restricted throughout life. At one year of age, comparisons of blood viscosity and haematology and changes in blood chemistry after exposure to thermal stress were conducted.

2. Whole blood viscosity was more than twice as high, and plasma triglyc‐eride and haemoglobin concentrations were over 30% higher in fat line than in lean line females fed ad libitum or in restricted birds of both genotypes.

3. Mean cell haemoglobin concentration was higher and mean cell volume lower in fat compared with lean line females.

4. Food restriction was associated with lower plasma triglyceride concentrations, lower numbers of heterophils and monocytes and a lower heterophil‐lymphocyte ratio.

5. Fat line birds had a higher blood pCO2 and lower pH than lean line birds.

6. Thermal stress was associated in ad libitum‐fed birds with a rise in blood pH and a decrease in pCO2, and in restricted birds with a decline in blood pH and an increase in pCO2.

7. Blood creatine kinase activity increased in all groups under thermal stress.  相似文献   


9.
1. The plasma viscosity values of normal commercial broiler and layer strains of chicken were measured in relation to age and sex.

2. In both broilers and layers plasma viscosity values decreased at 2 weeks of age; probably reflecting the period of change from maternal plasma protein to the bird's self‐synthesised proteins.

3. There was a progressive rise in plasma viscosity in both strains and sexes with age. The increase was greatest in female layers at 17 weeks of age before lay.

4. There was no overall significant difference in viscosity values between fresh and frozen‐stored plasma samples.  相似文献   


10.
1. The effects of hatch date, location and sex on the body weight and rate of gain in Coturnix coturnix japonica were examined.

2. The data conformed to a mixed model and adjustments were made for the fixed effects sex, hatch date and location. Adjustment improved the precision of the estimates by reducing the error variances significantly.

3. Rearing equal numbers of birds from each family at different locations made the interactions negligible.

4. Weight gain was greater if illumination intensity up to 35 d was less.

5. Females were not heavier on the 1st day but were heavier on the 14th and 35th days than males.

6. Variance components and genetic parameters were estimated.  相似文献   


11.
1. The effects of the dietary inclusion of a Bacillus-based direct-fed microbial (DFM) candidate on digesta viscosity, bacterial translocation, microbiota composition and bone mineralisation were evaluated in broilers consuming rye-based diets.

2. In the present study, control mash rye-based diets (CON) or Bacillus-DFM supplemented diets (TRT) were administered ad libitum to male broilers in three independent experiments.

3. In Experiments 1 and 2 (= 25/group), liver samples were taken to evaluate bacterial translocation, digesta samples were used for viscosity measurements and the intestinal microbial flora was evaluated from different intestinal sections to enumerate total recovered gram-negative bacteria (TGB), lactic acid bacteria (LAB) and anaerobic bacteria (TAB). Additionally, both tibias were removed for assessment of bone quality.

4. In Experiment 3, each experimental group had 8 replicates of 20 chickens (n = 160/group). Weekly, body weight (BW), feed intake (FI) and feed conversion ratio (FCR) were evaluated. At d 28-of-age, samples were taken to determine bacterial translocation, digesta viscosity and bone quality characteristics.

5. In all experiments, consumption of Bacillus-DFM reduced bacterial translocation to the liver and digesta viscosity. Additionally, DFM supplementation improved BW, bone quality measurements and FCR. Moreover, chickens fed on the Bacillus-DFM diet in Experiments 1 and 2 showed a significant reduction in the number of gram-negative and anaerobic bacteria in the duodenal content compared to control.

6. In summary, chickens fed on a rye-based diet without DFM inclusion showed an increase in bacterial translocation and digesta viscosity, accompanied by reduced performance and bone quality variables relative to the Bacillus-DFM candidate group. Hence, incorporation into the feed of a selected DFM ameliorated the adverse anti-nutritional effects related to utilisation of rye-based diets in broilers chickens.  相似文献   


12.
1. Embryos of the domestic fowl (72 h old) have been explanted into shell‐less cultures or ‘surrogate’ eggshells, in order to investigate the possibility of rearing these embryos to hatching.

2. Rocking embryo cultures during the first half of incubation enhanced embryo growth.

3. Embryos explanted into ‘surrogate’ eggshells of either other individuals or other species have been successfully ‘hatched’.

4. A normal chorioallantois is formed in these surrogate eggshells. This enables a functional albumen sac to form and eggshell resorption to be achieved.

5. Embryos grown in ‘surrogate’ eggshells are slightly smaller than controls but otherwise normal.

6. The technique provides opportunities for genetic engineering experiments.  相似文献   


13.
1. The effect of the ligation of the caeca on nitrogen utilisation and nitrogen excretion was examined in conventional chickens fed a diet containing 50 g protein/kg plus urea.

2. Ligation of the caeca significantly improved nitrogen balance and utilisation by up to more than 2 times as much as those of controls (P< 0.05).

3. The treatment significantly decreased uric acid excretion by 77 mg nitrogen/day (P< 0.01) and also total nitrogen excretion (P<0.05): the former decrease almost explained the latter.

4. No effect of the ligation of caeca on urea and ammonia excretion was observed.

5. It is concluded that nitrogen metabolism in chickens is affected by possible changes in caecal fermentation by preventing entry into the caeca of substances from urine and digesta.  相似文献   


14.
1. The paper compares between three genetically different lines in their egg weight and shell thickness response to a 28‐h cycle.

2. The response in shell thickness to ahemeral lighting was more rapid (2.2 d) than for egg weight (4.2 d).

3. Differences between genotypes were evident in both the speed and magnitude of response.

4. Overall, egg weight and shell thickness on the 28‐h cycle were 7% and 8% greater than on 24 h.

5. The allometric increase indicates that the increase in shell weight was on average 14% more than the increase in egg weight.  相似文献   


15.
1. Two hundred and sixty four dwarf broiler breeder hens were subjected to ad libitum or restricted feeding and to four lighting patterns: 15L (bright light):9D (dark), 15L:9d (dim light), 2L:10d: 1L:11d, (0.5L:3.5d) × 6.

2. Breeder hens fed ad libitum and subjected to either conventional or intermittent lighting ingested respectively, 25 g and 14 to 17 g more than hens restricted to 115 g/d.

3. Body weight was greater in hens fed ad libitum irrespective of the lighting pattern and of the amount of food intake.

4. Persistency of egg production was impaired by intermittent lighting.

5. Ad libitum feeding reduced egg fertility. The decrease was larger in breeder hens on intermittent lighting.

6. Hatchability was increased in hens submitted to the symmetrical lighting pattern (0.5L:3.5d).

7. Egg and chick weights were higher in hens fed ad libitum.

8. Shell index and shell breaking strength increased in restricted hens on the intermittent lighting pattern (0.5L:3.5d) × 6.

9. The best performance was obtained in restricted hens on the conventional lighting pattern.  相似文献   


16.
1. Separate balance experiments were conducted to assess the potential of 2 commercial enzyme supplements to improve the nutritive value of dehulled lupin kernels. One supplement (enzyme A) contained primarily xylanase, pen‐tosanase, hemicellulase activities and the other (enzyme B) primarily ß‐glucanase, hemicellulase and pectinase activities.

2. The enzymes were added at 0, 025, 0.50, 0.75 and 1.00 g/kg in diets containing (g/kg) lupins 300, sorghum 543, casein 91, celite (as marker) 20, and vitamins and minerals 46. Control diets, with and without enzyme supplementation contained sorghum and casein at 800 and 134 g/kg, respectively, and no lupins.

3. Growth rates and food conversion ratios (FCR) of birds over 7 days were not affected by lupin inclusion or enzyme supplementation. FCR of broilers fed on the sorghum diet was improved by enzyme A but not by enzyme B.

4. Heal starch digestibilities were slightly lower in birds fed on the lupin control diet (no enzyme) compared to the basal control diet.

5. Enzyme A increased the AME of the lupins from 10.01 MJ/kg DM to 11.65 MJ/kg DM when added at 0.5 g/kg. Higher rates of supplementation did not lead to further increases in AME values.

6. Enzyme A did not improve starch digestion in the diets but insoluble non‐starch polysaccharides concentration in the digesta decreased (50–41–42–71 g/g acid insoluble ash marker) with increasing enzyme supplementation, suggesting that the improvement in AME was the result of increased fermentation of fibre in the hindgut.

7. Enzyme B did not affect the AME of lupins nor the ileal digestibility of nutrients, but caused an increase in the concentrations of soluble non‐starch polysaccharides in the ileal digesta of chickens (19.21.35.77 mg/ml). This was accompanied by an increase in ileal digesta viscosity (11.4.34.2 m.Pa/s).  相似文献   


17.
1. This study investigated when and where body weight and flock‐uniformity should be determined in an aviary system by using automatic weighing systems.

2. An Individual Poultry Weighing System (IPWS) was developed to record time, duration, location and body weight of visits of individual hens to 4 weighing scales.

3. The number of hens that visited the weighing scales per 3 h period varied from less than 10 during the dark‐period to more than 60 during the light‐period.

4. The average number of visits per individual hen was 1–4 and the average number of successful weighings per hen was 0–6 during the light‐period.

5. Body weight showed a diurnal rhythm: the difference between the maximum body weight at night and the minimum body weight in the morning was 63 g.

6. The location of the scales influenced number of visits, number of weighings, mean body weight, flock‐uniformity and duration of visits.

7. Body weight per 3 h period did not differ between hens which were individually recognised and those which were not.

8. Flock‐uniformity was 2–6% higher during the light‐period if it was based on weighings of identified hen visits.

9. The average duration of the visits to the scales in the middle of the feeding tier during the light‐period was 63 s.

10. Of all the hens that visited the scales during a 24 h period, 54% visited them only once.

11. Automatic weighing systems without individual hen recognition can deliver reliable management information on mean body weight and flock‐uniformity in aviary systems if the weighing scales are located on the feeding tier in the middle of the house and if they are used during the light‐period.  相似文献   


18.
1. Fearfulness, shell colour, incidence and degree of shell whitening and the interval between ovipositions were studied in two populations of 30 brown egg laying hens with family histories of a low or a high incidence of egg shell whitening.

2. Hens of the population with the high incidence of whitening appeared to be more fearful than hens of the population with the low incidence of whitening.

3. Brown colouration of the egg shell and the incidence and degree of shell whitening declined as the hens aged.

4. Brown colouration and egg shell whitening were most pronounced on the blunt ends of the eggs.

5. A large part of the variation in egg shell whitening was attributable to the individual (hen) component of variance.

6. Differences in egg shell whitening, between the two populations, were detectable throughout the 26 weeks of the experiment.

7. Oviposition intervals were similar for normal and coated eggs when birds were not exposed to disturbance.

8. Disturbance of hens increased oviposition intervals and the incidence and degree of shell whitening, to a similar extent, in both populations.

9. It is concluded that stress‐related egg retention is not the sole factor responsible for abnormal egg shell whitening. Shell whitening may occur as a consequence of the premature termination of shell pigmentation as well as a consequence of the retardation of oviposition which occurs when hens are disturbed.  相似文献   


19.
The literature concerning crude fibre digestion and caecal function in poultry is briefly reviewed.

Digestibility trials are described, in which cockerels are compared before and after caecectomy; these birds after caecectomy are also compared with normal intact ones of the same age.

The trials showed a reduction in faecal dry matter after caecectomy, indicating that the caeca are active in absorption of water from the digesta. Faecal dry matter content seems to be more characteristic of the bird than of the food it eats.

There was a reduction in the overall digestibility of dry matter in the food after caecectomy, and also in that of crude fibre, though the latter effect is dependent on the food being eaten and on its crude fibre content.

Cellulose digestibility in a given bird was lowered, but the effect is not always evident between one bird and another, indicating that cellulose is digested bacterially in the caecum. No effect was found on pentosan and starch digestion.  相似文献   


20.
1. A choice between a high‐energy, wheat‐based, low protein mixture and a lower‐energy, soya‐based, high protein mixture was offered to growing Japanese quail at ambient temperatures of 20°, 25°, 30° and 35°C.

2. The quail were kept in open‐circuit respiration calorimeters, so that diet selection could be related to energy requirement.

3. Increasing ambient temperature had no significant effect on food intake by weight, but the proportion of the high energy choice decreased and, conversely, the proportion of the lower‐energy but higher‐protein choice increased.

4. Energy intake was therefore negatively correlated with ambient temperature, but protein intake per unit of energy intake increased, allowing the birds to gain weight at about the same rate at all temperatures.

5. Heat production decreased as ambient temperature increased. Respiratory quotient decreased with increasing temperature, which indicates a reduced utilisation of carbohydrate as an energy source.

6. Water intake increased with temperature but there were no overt signs of heat stress and there was no significant change in body temperature.

7. Japanese quail selected a dietary mixture which maintained similar growth rates over a wide range of ambient temperature, by sustaining protein intake but altering energy intake in line with thermoregulatory energy demands.  相似文献   


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