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1.
为研究日粮中添加不同水平的发酵饲料对育肥猪养分消化率及肠道菌群的影响,试验选用560头杜×长×大三元育肥猪[(75.37±2.65)kg],根据体重、性别随机分为7组,每组5个重复,每个重复16头,对照组饲喂育肥猪日粮,试验组分别在育肥猪日粮中添加5%或10%的发酵饲料A、B、C,饲喂21 d后每组选5头育肥猪肛门采集新鲜粪便测定肠道菌群多样性;并在饲喂第29天和第30天,各组每个重复连续收集粪便混合后测定养分消化率。结果表明:日粮中添加发酵饲料对育肥猪粗蛋白质、能量和干物质的消化率没有显著影响(P>0.05),相比对照组,饲喂发酵饲料组的粗蛋白质消化率有所提高(最高组提高2.9%);同时肠道菌群中乳杆菌属丰度有所增加,表明在日粮中添加一定比例的发酵饲料可以改善育肥猪养分消化率和肠道健康。  相似文献   

2.
本文旨在研究乳酸菌固态发酵饲料对生长育肥猪生长性能、肉品质和血液抗氧化能力的影响。试验选择体重和日龄相近的杜×长×大三元杂交生长育肥猪48头,随机分为对照组和试验组,每组6个重复,每个重复4头猪。对照组饲喂基础饲粮,试验组饲喂经乳酸菌发酵的基础饲粮。试验结果表明,乳酸菌发酵饲粮对生长期猪的生长性能无显著影响(P0.05)。乳酸菌发酵饲粮显著增加了育肥猪的平均日增重,降低了料肉比(P0.05),但对平均日采食量无显著影响(P0.05)。乳酸菌发酵饲粮显著提高了生长育肥猪血液的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性和抗氧化能力,显著降低血液丙二醛含量(P0.05)。乳酸菌发酵饲粮显著提高了生长育肥猪肉的亮度和红度(P0.05),改善猪肉颜色,降低了猪肉剪切力(P0.05),对p H无显著影响(P0.05),改善了肉质。因此,养猪生产实践中,可以给生长育肥猪饲喂乳酸菌固态发酵饲料,提高生长性能,改善猪肉品质。  相似文献   

3.
本试验旨在探讨生长育肥猪饲粮中添加玉米脱水酒精糟及其可溶物(DDGS)和复合酶制剂对生长育肥猪生产性能和氮、磷代谢的影响.试验选用200头70日龄杜×长×大三元杂交商品猪,根据体重和性别随机分成4个处理.每个处理5个重复,每个重复10头猪.分2阶段饲养(生长期和育肥期).对照组饲喂玉米-豆粕型饲粮,试验1组、试验2组和试验3组在生长期和育肥期饲料中分别用10%和15%的玉米DDGS替代部分玉米和豆粕,其中试验2组和试验3组添加200 g/t的复合酶制剂,试验3组的饲粮能量水平较前3组降低0.209 MJ/kg.结果显示,饲料中添加玉米DDGS,对生长育肥猪生产性能没有显著影响(P>0.05),粪氮含量显著提高(P<0.05),氮消化率显著降低(P<0.05),而粪磷含量显著降低(P<0.05).添加复合酶制剂在一定程度上提高了生长育肥猪生产性能和磷消化率(P>0.05),能量降低则显著提高了氮消化率(P<0.05).由此可知,适量添加玉米DDGS对生产性能没有负面影响,降低了饲料氮利用率,但提高了磷利用率.而辅以复合酶制剂则可在一定程度上提高动物对玉米DDGS的利用效率,提高生长育肥猪的生产性能和饲料磷消化率.  相似文献   

4.
本试验旨在研究饲粮中添加不同水平转猪生长激素(PGH)基因乳酸菌对断奶仔猪生长性能、免疫指标、粪中微生物及养分消化率的影响。试验共选择160头体重(7.56±0.56)kg的(21±2)日龄断奶仔猪,分为4个组,每个组4个重复,每个重复(栏)10头仔猪。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加0.1%、0.2%、0.3%的转PGH基因乳酸菌,试验期为28d。结果表明:1)与对照组相比,饲粮添加转PGH基因乳酸菌极显著提高断奶仔猪的末重和平均日增重(P0.01),显著降低料重比(P0.05);极显著降低各试验组的腹泻率与腹泻指数(P0.01)。2)与对照组相比,饲粮添加0.1%转PGH基因乳酸菌可显著或极显著提高血清免疫球蛋白A与补体4含量(P0.05或P0.01)。3)与对照组相比,饲粮添加转PGH基因乳酸菌显著降低粪中大肠杆菌数量(P0.05);加0.1%转PGH基因乳酸菌组极显著增加乳酸杆菌数量(P0.01)。4)与对照组相比,饲粮添加0.1%转PGH基因乳酸菌极显著提高干物质与粗蛋白质表观消化率(P0.01),显著提高总磷表观消化率(P0.05)。综合分析,饲粮中添加转PGH基因乳酸菌可提高断奶仔猪的生长性能和养分表观消化率,提高免疫力,调节肠道菌群平衡;添加水平为0.1%时效果最佳。  相似文献   

5.
文章旨在评估日粮添加不同水平的饲用枯草芽孢杆菌对9~24周龄育肥猪生长性能、养分表观消化及粪便特征的影响。将600头9周龄平均初始体重为(22.96±0.09)kg的商品猪随机分为3组,每组5个重复,每个重复40头。对照组饲喂基础日粮,T1和T2组肥猪分别饲喂基础日粮+100和200?mg/kg枯草芽孢杆菌(3×108?CFU/kg),试验共进行15周。结果:与对照组相比,T2组15?w育肥猪体重显著提高4.37%(P<0.05),同时T1和T2组1~15?w育肥猪的平均日增重较对照组分别显著提高3.81%和5.35%(P<0.05),饲料报酬分别显著提高4.44%和4.44%(P<0.05)。T1和T2组育肥猪干物质表观消化率分别较对照组显著提高8.75%和9.37%(P<0.05),同时T1组育肥猪有机物表观消化率较对照组显著提高了6.13%(P<0.05)。日粮添加100和200?mg/kg枯草芽孢杆菌可以显著降低6和15?w育肥猪粪便中氨气浓度和大肠杆菌数量(P<0.05),同时T1和T2组15?w育肥猪粪便中乳酸杆菌数量也显著高于对照组(P<0.05)。结论:日粮添加100~200?mg/kg饲用枯草芽孢杆菌(3×108?CFU/kg)可改善9~24周龄育肥猪的饲料报酬,改善干物质表观消化率,降低粪中氨气浓度和大肠杆菌数量。 [关键词]育肥猪|枯草芽孢杆菌|生长性能|养分消化|粪便特征  相似文献   

6.
试验旨在研究不同品质小麦日粮添加霉菌吸附剂对育肥猪生长性能、养分消化率及肠道相关基因表达的影响。选择86 d健康、体重接近[(38.08±0.56)kg]的杜×长×大商品猪各200头,随机分成4组,每组5个重复,每个重复10头。采用2×(低品质小麦和高品质小麦)2(3000 mg/kg和0 mg/kg霉菌吸附剂)设计试验日粮,试验共进行6周。结果显示:高品质小麦组显著提高了育肥猪平均日采食量(P0.05)和平均日增重(P0.05)。饲喂时间对育肥猪平均日采食量和料重比具有显著影响(P0.05)。育肥猪饲喂低品质小麦较高品质小麦显著降低养分表观消化率(P0.05),而日粮添加霉菌吸附剂较无霉菌吸附剂日粮显著提高养分表观消化率(P0.05)。饲喂高品质小麦日粮的生长猪较低品质小麦日粮显著降低结肠内容物大肠杆菌含量(P0.05),而日粮添加霉菌吸附剂较无霉菌吸附剂日粮显著提高结肠内容物乳酸杆菌含量(P0.05)。低品质小麦组较高品质小麦组添显著提高十二指肠和结肠TNF及十二指肠CLDN2基因m RNA表达水平(P0.05)。结果表明:低品质小麦日粮添加霉菌吸附剂可以提高营养物质的消化率,达到与高品质小麦日粮类似的结果。  相似文献   

7.
选用84头20 kg左右的生长猪,随机分为两个组,每个组3个重复,每重复14头。对照组饲喂添加抗生素200 mg/kg的日粮,试验组饲喂添加0.1%微生态制剂的日粮,试验期为28 d。试验结果表明,与对照组相比较,试验组生长猪的平均日采食量和平均日增重有增加的趋势(P>0.05),平均料肉比降低了0.34(P>0.05),腹泻率降低了4.94%(P<0.01);在提高生长猪日粮养分消化率方面,微生态制剂具有与抗生素相同的作用(P>0.05);粪样中的大肠杆菌数量显著降低(P<0.05),同时粪样中乳酸菌和双歧杆菌数量都有升高的趋势(P>0.05)。  相似文献   

8.
本试验在生长育肥猪日粮中添加酶解蛋白肽T30,研究小肽对生长育肥猪的生长性能、血液生化指标及养分表观消化率的影响,并探讨其在生长育肥猪阶段的最适添加量。采用单因子随机试验设计。选取杜洛克猪×长白猪×大白猪三元商品猪300头,随机分为5组,每组4个重复,每个重复15头。Ⅰ组(对照组)饲喂基础日粮,Ⅱ~Ⅴ组在基础日粮中分别添加1%、2%、3%、4% T30。试验期30 d。结果显示:①Ⅲ组的平均日增重显著高于Ⅰ、Ⅴ组(P<0.05);Ⅲ组平均日采食量显著高于Ⅱ组(P<0.05),与其他组间差异均不显著(P>0.05)。②Ⅴ组总蛋白、球蛋白水平最高,显著高于Ⅱ组(P<0.05),但与其他各组间差异均不显著(P>0.05);Ⅱ组尿素氮水平显著低于其他各组(P<0.05)。③Ⅴ组干物质、粗蛋白质表观消化率最高,T30添加组钙、磷表观消化率均出现不同程度的下降。综上所述,在生长育肥猪阶段酶解蛋白肽的最佳添加量为1.796%,添加酶解蛋白肽可提高生长性能、降低料重比,提高血液总蛋白水平、球蛋白水平。  相似文献   

9.
本试验旨在研究无抗发酵饲料对生长育肥猪生长性能、肠道菌群和养分表观消化率的影响。选择18 kg左右断奶仔猪60头,随机分为2个组,每组3个重复,每个重复10头猪。对照组饲喂含抗生素基础饲粮(每千克含黄霉素5 mg),试验组饲喂80%基础饲粮和20%的无抗发酵饲料组成的试验饲粮。试验期154 d。结果表明:1)试验组的末重、平均日增重和平均日采食量都显著高于对照组(P0.05),而料重比则显著低于对照组(P0.05)。2)试验组中期和后期的乳酸菌数量的对数值显著高于对照组(P0.05),而试验组中期和后期的大肠杆菌和沙门氏菌数量的对数值显著低于对照组(P0.05)。3)在十二指肠肠段,试验组肠道食糜中的乳酸菌和大肠杆菌数量的对数值显著高于对照组(P0.05)。4)在十二指肠肠段,试验组肠道每克和每平方厘米黏膜的乳酸菌数量的对数值显著高于对照组(P0.05);试验组肠道每克黏膜的大肠杆菌数量的对数值显著高于对照组(P0.05)。在空肠肠段,试验组肠道每克和每平方厘米黏膜的乳酸菌数量的对数值显著高于对照组(P0.05)。5)试验组的粗蛋白质和粗纤维的表观消化率显著高于对照组(P0.05)。由此可见,无抗发酵饲料能提高生长育肥猪的生长性能,改善肠道微生物平衡,增强消化能力。  相似文献   

10.
本试验旨在研究水合硅铝酸钠钙(HSCAS)对生长育肥猪生长性能、养分表观消化率及抗氧化能力的影响。选择(48.0±10.0)kg的健康"杜×长×大"生长猪45头,随机分为3个处理,每个处理5个重复,每个重复3头猪。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加0.25%和2.50%HSCAS的饲粮。试验期为70d,分为48~80kg(前期)和81~110kg(后期)2阶段进行。结果表明:1)与对照组相比,添加0.25%HSCAS对猪生长性能、血清尿素氮(UN)含量和抗氧化能力均无显著影响(P>0.05),但显著降低了后期粗蛋白质(CP)和能量的表观消化率(P<0.05);2)与对照组相比,添加2.50%HSCAS显著提高了后期和全期的料重比(P<0.05),显著提高了血清UN含量(P<0.05),显著降低了后期生长育肥猪CP、粗灰分和能量的表观消化率、血清超氧化物歧化酶活性及总抗氧化能力(P<0.05)。由此可知,饲粮中添加0.25%HSCAS对生长育肥猪的生长和健康无明显影响,但添加量达到2.50%可显著降低其生长性能、养分表观消化率和抗氧化能力。  相似文献   

11.
12.
Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size.  相似文献   

13.
In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum.  相似文献   

14.
This study was conducted to measure the concentrations of strontium (Sr), barium (Ba), cadmium (Cd), copper (Cu), zinc (Zn), manganese (Mn), chromium (Cr), antimony (Sb), selenium (Se), and lead (Pb) in canine liver, renal cortex, and renal medulla, and the association of these concentrations with age, gender, and occurrence of chronic kidney disease (CKD). Tissues from 50 dogs were analyzed using inductively coupled plasma mass spectrometry. Cu, Zn, and Mn levels were highest in the liver followed by the renal cortex and renal medulla. The highest Sr, Cd, and Se concentrations were measured in the renal cortex while lower levels were found in the renal medulla and liver. Female dogs had higher tissue concentrations of Sr (liver and renal medulla), Cd (liver), Zn (liver and renal cortex), Cr (liver, renal cortex, and renal medulla), and Pb (liver) than male animals. Except for Mn and Sb, age-dependent variations were observed for all element concentrations in the canine tissues. Hepatic Cd and Cr concentrations were higher in dogs with CKD. In conclusion, the present results provide new knowledge about the storage of specific elements in canine liver and kidneys, and can be considered important reference data for diagnostic methods and further investigations.  相似文献   

15.
《饲料工业》2019,(18):54-58
应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0~1 000 ng/ml范围内线性良好,仪器检出限为0.557 7~5.072 ng/ml,具有良好的精密度,其回收率在88.1%~104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。  相似文献   

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A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays.  相似文献   

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Circular excised skin wounds in the thoracic and metatarsal regions of the dog were studied. A similar sequence of events took place in the two regions although differences did occur due to the different reactions of the tissues which surrounded the wounds. None of the wound cavities became filled with exudate during the early stages of healing. In the thoracic wounds the cavities were largely filled by the swelling and inward movement of adipose tissue. Epithelium first grew on the wound surface in the sector of the wound that was situated in the direction of hair flow. The average time to complete epithelization was similar in both sets of wounds. A zone of alopecia developed around the wounds.  相似文献   

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