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1.
应用布鲁氏菌病虎红平板试验(Rose-Bengal Plate Agglutination Test,RBPT)、衣原体和弓形虫间接血凝试验(Indirect Hemagglutination Assay,IHA),对来自青海省祁连县某藏羊场的53只藏系后备公羊血清进行特异性血清抗体的检测,结果检出衣原体阳性血清3份,阳性率为5.75%;弓形虫阳性血清5份,阳性率9.4%,未检出布氏杆菌。  相似文献   

2.
应用布鲁氏菌病RBPT、衣原体和弓形虫IHA,对来自青海省三角城种羊场发生流产的23只生产母羊血清进行特异性血清抗体的检测,结果检出衣原体阳性血清3份,阳性率为13.04%:未检出布氏杆菌和弓形虫阳性血清。  相似文献   

3.
采用间接血凝(1HA)试验对103份种公羊血清样品,100份种公牛血清样品、200份生产母羊血清样品进行检测,共检出种公羊阳性3份,阳性率2.91%;检出种公牛阳性2份,阳性率2%;检出生产母羊阳性5份,阳性率2.5%。  相似文献   

4.
用布氏杆菌试管凝集试验(SAT),对海晏地区的绵羊进行了布氏杆菌血清抗体的检测,共检397份血清样品,检出阳性血清2份,阳性率为0.50%;其中:成年母羊血清233份,检出阳性1份,阳性率为0.43%;后备母羊血清91份,检出阳性1份血清,阳性率为1.10%;羯羊血清52份,阳性率为0%;种公羊21份,也未检出阳性。  相似文献   

5.
通过虎红平板凝集试验(RBPT)、弓形虫和衣原体间接血凝试验(IHA),对来自青海省互助县某羊场的19份母羊血清进行了布鲁氏菌、弓形虫和衣原体的血清抗体检测.结果检出衣原体阳性血清1份,阳性率为5.3%;未检出布鲁氏菌和弓形虫的阳性血清.流产胎儿瘤胃液,经PCR检测,为衣原体感染.  相似文献   

6.
应用虎红平板凝集试验(Rose-Bengal Plate Agglutination Test,RBPT)、衣原体、弓形虫、绵羊支原体间接血凝试验(Indirect Hemagglutination Assay,IHA),对采自青海省祁连县两个村绵羊的199份血清进行了布氏杆菌、衣原体、弓形虫的血清抗体检测。结果:在199份被检血清中,未检出布氏杆菌和衣原体的血清阳性;检出弓形虫阳性血清9份,阳性率为4.5%;羊传染性胸膜肺炎阳性血清13份,阳性率为6.5%。  相似文献   

7.
青海省海晏县绵羊衣原体病的调查   总被引:1,自引:0,他引:1  
应用衣原体间接血凝试验(IHA)进行了青海省海晏县绵羊衣原体病血清学调查,共检测237份生产母羊血清均检出阳性13份,阳性率为5.49%。其中:哈勒景乡的生产母羊血清77份,检出阳性4份,阳性率为5.19%;金滩乡的生产母羊血清81份,检出阳性6份,阳性率为7.41%;银滩乡的生产母羊血清79份,检出阳性3份,阳性率为3.80%。  相似文献   

8.
应用虎红平板凝集试验和间接血凝试验对采自加定地区部分雪灾后发生流产,且仍然健活的48份绵羊血清样品,进行布氏杆菌、衣原体病和弓形虫特异性抗体检测,结果:未检出布氏杆菌和衣原体;但检出弓形虫病,检出阳性血清2份,阳性率为4.17%。  相似文献   

9.
王敏 《家畜生态》2011,(6):80-81,123
采用布氏杆菌虎红平板凝集试验、弓形虫和衣原体间接血凝试验对360份绒山羊血清进行布氏杆菌病、弓形虫病和衣原体病的血清学调查。结果在绒山羊的血清样品中检出布氏杆菌病阳性血清8份,布氏杆菌病的血清阳性率为2.22%;检出弓形虫15份,弓形虫病血清阳性率为4.17%;检出衣原体23份,衣原体病血清阳性率为6.39%。  相似文献   

10.
应用虎红平板凝集试验(Rose-Bengal Plate Agglutination Test,RBPT)、衣原体、弓形虫和绵羊肺炎支原体间接血凝试验(Indirect Hemagglutination Assay,IHA),对2013年采自青海省同德县部分乡镇的201份绵羊血清样品进行了布鲁氏菌、衣原体、弓形虫和绵羊肺炎支原体的血清学检测。结果:未检出布氏杆菌阳性血清;分别检出衣原体、弓形虫和绵羊肺炎支原体阳性血清2份、4份和7份,其阳性率分别为1.00%、1.99%和3.48%。此外,对于抗体阳性的血清样品采用PCR方法进行了抗原检测,结果表明,青海省同德县地区绵羊中存在衣原体、弓形虫和绵羊肺炎支原体的感染。  相似文献   

11.
应用绵羊正向间接血凝试验对来自青海省三角城种羊场的59份试情公羊的血清样品,进行羊传染性胸膜肺炎的血清学调查。结果检出阳性血清5份,阳性率为8.48%,表明青海省三角城种羊场的羊群中存在传染性胸膜肺炎的感染。  相似文献   

12.
青海改良种绵羊弓形体病的血清学调查   总被引:2,自引:1,他引:2  
应用间接血凝试验(IHA),对采集的160份青海改良种绵羊血清,进行了弓形体病的血清学抗体定性检测。结果检出7份阳性血清,血清学阳性率为4.38%。  相似文献   

13.
AIM: To determine the effect of the age of rams, used at a standardised ram-to-ewe ratio, on the breeding performance and pregnancy rate of ewe hoggets. METHODS: Ewe hoggets ((1/2) Romney x (1/4) Texel x (1/4) Finnish Landrace; n=582) aged 7-8 months were exposed to nine two-tooth, harnessed, vasectomised teaser rams for a period of 17 days (Days 1-17). The ewe hoggets were then weighed and randomly assigned to one of three treatment groups (on Day 18) and joined with either four ram hoggets not used previously (n=194), four two-tooth rams not used previously (n=196), or four mixed-age mature rams (n=192) that had been used previously to mate mature ewes immediately before breeding with the ewe hoggets. The breeding period was 34 days. Ewe hoggets were identified as having been mated during the first 17 days only, during both 17-day periods, during the second 17 days only, or not mated. All ewe hoggets were scanned for pregnancy using ultrasound, 54 days after the end of the breeding period. RESULTS: Liveweights of ewe hoggets did not differ between treatment groups. More ewe hoggets were mated by the mature rams in the first 17 days than by either ram hoggets or two-tooth rams (p<0.05). However, more of the ewe hoggets mated by mature rams returned to service (failed to conceive), and the percentage of ewe hoggets bred in the first 17 days only or pregnant from a service in the first 17 days did not differ significantly between groups. Fewer of the ewe hoggets joined with ram hoggets were diagnosed as pregnant after 34 days of breeding compared with those joined with either two-tooth or mature rams (p<0.05), as more ewe hoggets failed to be mated at all by ram hoggets compared with those joined with mature rams (p<0.05). There was also a tendency for more ewe hoggets joined with ram hoggets not to be mated at all compared with those joined with two-tooth rams (p=0.07). CONCLUSIONS: Two-tooth rams previously unused and mature rams previously used for mating were a better option than unused ram hoggets to maximise the pregnancy rate of ewe hoggets to a 34-day breeding period. However, further work is required as it was unclear why lower conception rates were observed in ewe hoggets joined with mature rams which had been used previously. As a result, the advantage of using mature rams may have been underestimated.  相似文献   

14.
白萨福克羊高原过渡期适应性研究   总被引:1,自引:0,他引:1  
以过渡阶段饲养的27只白萨福克羊为研究对象,分析其生理指标、血液生理生化指标、行为学、生长发育、繁殖性能及抗病性等,研究引入的白萨福克羊在向高原过渡饲养阶段的适应性.结果表明:白萨福克羊的生理指标和血液生理生化指标与我国绵羊相比存在一定差异,在正常波动范围内,红细胞平均容积公母羊之间差异显著(P<0.05),其他19项指标公母羊间差异不显著(P>0.05);公母羔羊初生重分别为(5.33±1.15) kg和(4.88±0.15) kg,初生至3月龄时生长速度较快,3~9月龄时逐步减慢,公母羊成年体重分别为(88.00±6.36)kg和(62.06±6.40) kg;过渡期间白萨福克羊的产羔率为145%,对疾病抵抗力较强.白萨福克羊适应于过渡饲养阶段的环境,为转运至青藏高原地区饲养提供依据.  相似文献   

15.
[目的] 评价杜蒙羊的肉用性能、屠宰性能及肉品质。[方法] 选取60只杜蒙羊,公母各半,于5月龄时利用超声波背膘检测仪对羊只进行活体肉用性能检测,于6月龄时对羊只进行屠宰,测定主要屠宰性能指标以及肉品质和安全性评价指标;对杜蒙羊公羊和母羊的活体肉用性能指标、主要屠宰性能指标、肉品质和安全性评价指标进行统计学分析和比较。[结果] 杜蒙羊活体肉用性能指标检测结果表明,公羊的腰肌厚度和眼肌面积显著(P<0.05)大于母羊,背膘厚度与母羊差异不显著(P>0.05);屠宰后肉用性能指标测定结果表明,公羊的背脂厚和眼肌面积显著(P<0.05)大于母羊。屠宰性能指标测定结果表明,杜蒙羊公羊的胴体重、净肉重、宰前活重均显著(P<0.05)高于母羊,公羊的屠宰率、胴体净肉率高于母羊,但无显著(P>0.05)差异;母羊的大腿骨重、小腿骨重、大腿骨长、小腿骨长均显著(P<0.05)低于公羊,公羊和母羊的4个骨指标两两之间呈正相关,大腿骨重和小腿骨重更接近线性相关。肉品质和安全性评价指标测定结果表明,杜蒙羊公羊的肌内脂肪含量显著(P<0.05)低于母羊;公羊的胴体肌肉水分含量与母羊差异不显著(P>0.05);公羊和母羊肌肉中的铅、铬、汞、砷元素含量均符合《农产品安全质量 无公害畜禽肉安全要求》(GB 18406.3—2001),母羊肌肉中的铬含量和汞含量显著(P<0.05)低于公羊。[结论] 杜蒙羊公羊的活体肉用性能和屠宰性能优于母羊,母羊的肉品质优于公羊。  相似文献   

16.
AIM: To determine the effects of the ratio of vasectomised (teaser) rams to ewe hoggets used prior to breeding on the breeding performance and pregnancy rate of ewe hoggets. METHODS: Romney-composite ewe hoggets (n=1,180), 7-8 months of age, were randomly assigned to one of five treatment groups (Day 1) and either exposed to harnessed teaser rams at a teaser-ram:ewe-hogget ratio of 1:32 (n=193 hoggets), 1:66 (n=199), 1:98 (n=197), or 1:197 (n=395), for 17 days prior to the breeding period, or not exposed to teasers (unteased; n=196). Harnessed entire rams were then introduced for the duration of two oestrous cycles (34 days). Using crayon marks, ewe hoggets were identified as having been mated during the first 17 days only, during the second 17 days only, during both 17-day periods, or not mated. Hoggets were weighed on Days 1 and 18. All ewe hoggets were scanned for pregnancy using ultrasound, 57 days after the end of the breeding period. RESULTS: Hogget groups did not differ in liveweight at Day 1, but at Day 18 hoggets exposed to teasers at a ratio of 1:66 were lighter, and those exposed to teasers at a ratio of 1:32 were heavier than all other groups by up 3.0 kg (p<0.05). Unteased ewe hoggets were less likely to be pregnant to the first 17 days of breeding compared with all teased groups, by at least 16.4% (p<0.05). Over the entire 34-day breeding period, however, only those teased at a ratio of 1:32 were more likely to be pregnant, by 11.9%, than unteased hoggets (p<0.05). Within the teased hogget groups, there were no differences between groups for either the percentage mated in the first 17 days of breeding only, or those pregnant to either the first 17 days of breeding only or the entire breeding period. CONCLUSIONS: The results of this study indicate that teaser-ram:ewe-hogget ratios as high as 1:197 can increase the percentage of ewe hoggets bred in the first 17 days of mating by at least 16.4%. Across the range of ratios used there was little difference in breeding pattern or pregnancy rate, although results might suggest ratios 1:98.  相似文献   

17.
运用ELISA、LAT和IHA对湖北省2006年度规模化猪场猪伪狂犬病(PR)、猪繁殖与呼吸综合征(PRRS)、猪乙型脑炎(JE)、猪细小病毒病(PP)、猪瘟(HC)、猪圆环病毒病(PCV-2)及弓形体病进行了血清学检测。分析调查结果发现PR、PRRS、JE、PP 、HC 5种疾病的免疫抗体平均阳性率分别为84.3%、78.3%、84.7%、58.0%、79.7%。非免PRRSV和PRV野毒的平均感染率分别为55.1%和17.7%,表明PRRSV感染情况比较严重。对58场次发病猪场的541份血清进行PRV、PRRSV、PCV-2混合感染情况的调查发现,规模化猪场中普遍存在二重或三重感染,从而使疾病更加复杂化,给防疫工作带来更大的困难。  相似文献   

18.
[目的]确定羊同期发情腹腔镜输精的最佳时间。[方法]选取繁殖机能正常、营养状况良好的适龄母羊,在试验母羊阴道内放置孕酮海绵栓10~14 d,撤栓时肌肉注射250~330 IU/只孕马血清促性腺激素(PMSG)。撤栓后12~24 h开始试情,早晚各1次。按撤栓后时间及发情时间不同分4组进行腹腔镜输精:1组为撤栓后46~50 h输精(n=138),2组为撤栓后50~54 h输精(n=156),3组为撤栓后54~58 h输精(n=275),4组为羊发情后30~36 h输精(n=226)。输精后第40~45天进行B超检查,计算各组母羊受胎率。[结果]发情后30~36 h输精的母羊(4组)受胎率最高,为97.79%,其次为撤栓后50~54 h输精的母羊(2组),受胎率为97.44%,撤栓后46~50 h输精的母羊(1组)受胎率为91.30%;撤栓后54~58 h输精的母羊(3组)受胎率最低,为89.82%。[结论]采用同期发情配合腹腔镜输精技术进行母羊人工授精,最佳输精时间为撤栓后50~54 h以及母羊发情后30~36 h。  相似文献   

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