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一株强毒大肠杆菌的分离与鉴定 总被引：3，自引：0，他引：3
将剖检见典型大肠杆菌病症状的鸡肝中分离出的细菌通过细菌分离培养及镜检、生化试验、动物接种试验、药敏试验进行鉴定,确诊该菌为大肠杆菌。将其进行动物接种,结果证明,３．０×１０７个细菌能使３日龄的雏鸡在８天内全部死亡,并从病死鸡肝脏中分离出纯大肠杆菌而不见其它杂菌。该菌是一株强毒大肠杆菌。 相似文献
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从重庆武隆某种猪场分裂得到8株疑似大肠杆菌,经染色镜检、糖发酵试验、生化试验、溶血性试验、小白鼠试验,证明分裂株为致病性大肠杆菌,通过药敏实验,筛选出菌必治、先锋噻肟、头孢唑啉、阿米卡星对该猪场所分裂出的致病性大肠杆菌敏感。 相似文献
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试验通过对某地区从60例疑似病死鸡体内分离出55株细菌,经生化鉴定50株属大肠杆菌,并筛选出具有代表性的5个鸡场分离的大肠杆茵做药敏试验,以期了解该地区鸡大肠杆茵耐药性情况,从而为该地区防治鸡的大肠杆菌病提供科学依据．为今后科学合理的防治禽大肠杆菌病积累经验。 相似文献
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为探讨长春地区某仓鼠饲养场中仓鼠急性死亡的原因及应对该突发性疾病,对死亡仓鼠进行实验室检查并分离得到1株细菌,通过对该分离株进行形态学观察、培养特性观察、生化试验、小鼠致病性试验、PCR鉴定和23SrRNA基因同源性分析,判定该分离菌为1株高致病性大肠杆菌。该菌可致仓鼠突发急性致死性大肠杆菌病,死亡率达到100%,死亡小鼠出现胃肠道极度膨胀的典型症状。药敏试验结果表明,该大肠杆菌对多种抗生素敏感,但对四环素类抗生素及复方新诺明(SXT)有很强的耐药性,可用庆大霉素进行治疗。研究结果为探讨急性大肠杆菌病的发病机制及其防治提供了理论基础。 相似文献
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自发病眼镜蛇体内分离的细菌经形态学观察、培养特性及生化试验,鉴定为大肠杆菌。该大肠杆菌做动物试验,可致小白鼠死亡,说明其具有一定的毒力。用临床上常用的14种抗菌素纸片对分离菌株进行药敏试验,筛选出敏感药物用于临床治疗取得良好效果,有效控制了眼镜蛇大肠杆菌病的流行。 相似文献
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鹅大肠杆菌的分离鉴定及药敏试验 总被引：1，自引：0，他引：1
为了对鹅大肠杆菌的临床治疗提供指导并为制备大肠杆菌自家灭活疫苗奠定基础,采集疑似病例的病料,用麦康凯培养基分离细菌,对分离的细菌进行形态学鉴定、生化鉴定、血清型鉴定、致病性试验及药敏试验。结果显示,所分离到的菌落呈粉红色、光滑湿润、边缘整齐;革兰氏染色为阴性小杆菌,两端钝圆,多单个存在;共鉴定出15株大肠杆菌,其中4株O35型,3株O78型,3株O26型,2株O7型,1株O109型,1株O141型,1株O2型;动物试验表明所鉴定的菌株对小鼠和雏鹅均有较高的致死率;体外抑菌试验结果发现强力霉素、阿莫西林对该场的鹅致病性大肠杆菌具有较强的抑制作用。本研究为有效防制鹅大肠杆菌病提供了理论依据,对于控制规模化鹅场大肠杆菌等细菌性疾病具有重要的应用价值。 相似文献
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黑龙江省某规模化奶牛场部分新生犊牛发生了严重腹泻且犊牛病死率较高。为了确诊,无菌采集病料,通过病毒检测、细菌分离、生化试验、细菌和病毒特异性基因PCR扩增、动物致病性试验及药敏试验等方法进行了病毒抗原和微生物检测。结果显示,病毒检测中牛轮状病毒（BRV）呈阳性,PCR扩增得到400 bp的特异性片段;从病料中分离到1株含K99和F41兼性菌毛抗原的高致病性产肠毒素大肠杆菌（ETEC）,该分离菌株对恩诺沙星、头孢唑啉、氯霉素敏感,对其他抗生素有一定的耐药性。流行情况调查结合病原学检查确诊该牛场新生犊牛严重腹泻是由BRV和大肠杆菌混合感染造成的,根据诊断结果进行了针对性地防制,取得了良好的防控效果。 相似文献
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LI Yan-ting SUN Guang-ye ZHANG Lu WANG Shan-shan GAO Guo-qiang HOU Xi-lin 《中国畜牧兽医》2017,44(1):248-253
Newborn calves in a dairy farms suffered from severe diarrhea disease with high mortality in Heilongjiang province.In order to make a definite diagnosis,the pathological samples were collected aseptically and the viral antigens and microorganism were detected by virus detection,bacteria separation,biochemical test,PCR for the specific gene of virus and bacteria,animal pathogenicity test and antimicrobial susceptibility test. The results showed that the virus detection of bovine rotavirus was positive and specific gene fragment of bovine rotavirus was amplified. One E. coli strain with K99 and F41 adhesin antigens which belonged to highly pathogenic enterotoxigenic E. coli was isolated. This strain was sensitive to enrofloxacin,cefamedin and chloramphenicol,however,it showed resistance for other antibiotics. A polyinfection of bovine rotavirus and E. coli were eventually confirmed through epidemic investigation and pathological diagnosis. It had significant preventive effect by taking effective measures according to the diagnosis results. 相似文献
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Hart WS Heuzenroeder MW Barton MD 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2006,53(7):333-340
Experiments to demonstrate the transfer of genes within a natural environment are technically difficult because of the unknown numbers and strains of bacteria present, as well as difficulties designing adequate control experiments. The results of such studies should be viewed within the limits of the experimental design. Most experiments to date have been based on artificial models, which only give approximations of the real-life situation. The current study uses more natural models and provides information about tetracycline resistance as it occurs in wild-type bacteria within the environment of the normal intestinal tract of an animal. Tetracycline sensitive, nalidixic acid resistant Escherichia coli isolates of human origin were administered to mice and chicken animal models. They were monitored for acquisition of tetracycline resistance from indigenous or administered donor E. coli. Five sets of in vivo experiments demonstrated unequivocal transfer of tetracycline resistance to tetracycline sensitive recipients. The addition of tetracycline in the drinking water of the animals increased the probability of transfer between E. coli strains originating from the same animal species. The co-transfer of unselected antibiotic resistance in animal models was also demonstrated. 相似文献
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大肠杆菌毒力因子研究概况 总被引：17，自引：0，他引：17
致病性大肠杆菌的毒力因子主要有黏附素、毒素、外膜蛋白 ( OMP)及铁转运系统等。在大肠杆菌侵袭宿主组织并引起其发病的过程中 ,这些毒力因子相互协调、发挥作用。文章对毒力因子的致病作用、致病机理、免疫原性及其在疾病防制中的应用几个方面的研究概况作了综述 ,为防制大肠杆菌病、研制新型高效的大肠杆菌疫苗提供了理论依据。另外 ,文章还介绍了近年来已相继研制成功的全菌体疫苗、纯化菌毛疫苗、单价或多价基因工程菌毛疫苗以及重组肠毒素双价基因工程苗、OMP亚单位疫苗等。展现了新型疫苗的研制 ,给防制大肠杆菌病带来了新的前景以及大肠杆菌作为工程菌的应用对畜禽其他疾病防制的推动作用 相似文献
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232 isolates of gram-negative, oxidase-negative bacteria, isolated from various samples of different animal species, were tested with help of RAPIDEC coli for the production of beta-glucuronidase, beta-galactosidase and indole. The test correctly identified 164 of 175 E. coli strains (sensitivity 93.7%) and correctly indicated that 57 of 57 isolates of the family Enterobacteriaceae (7 Citrobacter sp., 18 Enterobacter sp., 16 Klebsiella sp., 10 Proteus sp., 2 Providencia sp. and 4 Salmonella sp.) were not E. coli (specificity 100%). 相似文献
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Natural infection with an attaching and effacing Escherichia coli in a diarrheic puppy. 总被引：4，自引：0，他引：4 
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下载免费PDF全文 A Broes R Drolet M Jacques J M Fairbrother W M Johnson 《Canadian journal of veterinary research》1988,52(2):280-282
Enteric infection with an attaching and effacing Escherichia coli was diagnosed in a puppy with protracted diarrhea. Extensive colonization of the small intestinal mucosa was observed by light and scanning electron microscopy and characteristic lesions of bacterial attachment of the brush border of the enterocytes were demonstrated by transmission electron microscopy. The E. coli strain isolated from the small intestine belonged to serotype O49:H10, did not produce any known E. coli enterotoxin or cytotoxin, was not invasive, and was negative for the known fimbrial colonization factors produced by animal and human enterotoxigenic E. coli. A positive immunoperoxidase reaction was obtained on the bacteria attached to the enterocytes with an anti-E. coli O49 antiserum. 相似文献
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Chitrita DebRoy Elisabeth Roberts 《Journal of veterinary diagnostic investigation》2006,18(6):597-600
Several outbreaks of Escherichia coli O157 have been reported in petting zoos, resulting in hospitalization of many children. At present, no standard procedure has been adopted to monitor the presence of enterohemorrhagic E. coli (EHEC) or Shiga-toxin-producing E. coli (STEC) in petting zoo animals. Direct detection of these strains from rectal swabs of animals in petting zoos was developed and obviated the need to culture the organisms. DNA extracted from bacteria in the swabs was tested for the presence of wecA gene specific for E. coli by polymerase chain reaction (PCR). The wecA positive samples were further tested for Shiga-toxin genes stxl and stx2, and the intimin eae by multiplex PCR and for the presence of O157 and H7. Swabs (n=104) from 15 animal species in a petting zoo were tested; 7 goats and 3 cows were found to carry STEC. The method is rapid and convenient for monitoring potentially pathogenic E. coli in petting zoo animals. 相似文献
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Coliform mastitis (CM) is not only a serious economical and animal welfare touching problem in dairy cattle, but also in sows after farrowing. Due to this disease, the essential adequate supply with colostrum for the growth and the health of the piglets is not ensured. Besides other influencing factors, Escherichia (E.) coli is of great importance as a causative agent of this multifactorial disease. In this study, E. coli isolates from milk samples of healthy and CM-affected sows were examined for the presence of virulence genes associated with extraintestinal E. coli strains, enterotoxigenic E. coli and other pathogenic E. coli. The isolated E. coli harbored mainly virulence genes of extraintestinal E. coli strains (especially fimC, ompA, traT, hra, kpsMTII, iroN). The virulence gene spectrum for both samples from CM-affected and healthy sows did not differ significantly. Particular virulence gene profiles of E. coli isolates from diseased sows were not detected. This study provides novel insights into the role of E. coli in association with mastitis in sows since it is the first time E. coli isolates from CM-affected sows' milk were analysed for virulence genes. Because there were no differences in the prevalence of E. coli and their virulence-associated genes between healthy and diseased sows, other causative factors seem to have greater influence on the pathogenesis of porcine CM. 相似文献