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1.
何楚  张小敏  周斌  姜焱 《畜牧与兽医》2012,44(12):92-95
盖他病毒是披膜病毒科甲病毒属成员的一种单链线形RNA病毒。盖他病是由蚊虫传播的一种虫媒传染病,主要侵害马和猪,本病多发生于亚洲,特别是东南亚地区。近年来在我国部分省区蚊虫中分离到盖他病毒,表明该病毒在我国分布较为广泛。目前,关于盖他病毒的研究还较少,很多机理尚未清楚,其危害程度亦不可预知。为了更好地掌握盖他病毒的研究进展,综合查阅相关文献资料,对该病毒的分子生物学特性做了一些简要总结概括,为建立和完善人畜共患病公共卫生体系和早期预警机制提供一些知识贮备。  相似文献   

2.
正猪乙型脑炎是由日本脑炎病毒引起的一种人畜共患传染病,临床症状主要表现为母猪流产、产死胎,公猪表现为睾丸炎。该病毒对温度敏感,56℃30 min即可灭活,在冻干状态或-70℃可存活数年,2%苛性钠、3%来苏儿等常用消毒药均可杀灭和灭活该病毒,病毒对胰酶、乙醚、氯仿等化学试剂亦较为敏感。乙脑病毒是一种有囊膜,含有糖蛋白纤突的单股RNA病毒,仅有一个开放阅读框,经蛋白酶水解后  相似文献   

3.
猪圆环病毒(porclne Circovirus)于1974年在PK-15细胞中发现,当时认为是一种细胞污染物,后证实为一种新的病毒.该病毒是一种环状、无囊膜、单链DNA病毒;在ICTV第6次病毒分类报告中,将猪的圆环病毒 (PCV)、鸡贫血病毒(cAV)、鹦鹉喙羽病毒(PBFDAV)3个病毒归类于圆环病毒科,圆环病毒属成员.  相似文献   

4.
水貂阿留申病病毒分子生物学研究进展   总被引:3,自引:0,他引:3  
水貂阿留申病病毒是一种在水貂中广泛存在的重要病原体.该病毒属阿留申病毒属,主要编码4种蛋白(结构蛋白VP1、VP2和非结构蛋白NS1、NS2).VP1蛋白在协助病毒产生感染性方面起着重要作用;VP2蛋白是该病毒的主要免疫原性抗原,能体外中和病毒;NS1和NS2对病毒在宿主细胞中的复制起重要的调节作用.该病毒的分子生物学诊断技术主要有核酸杂交技术、PCR和基因芯片检测技术.  相似文献   

5.
鸭病毒性肝炎又称鸭肝炎,是由鸭肝炎病毒引起的雏鸭的一种高度致死性传染病.鸭肝炎病毒分为Ⅰ、Ⅱ、Ⅲ三种血清型,各型之间无交叉免疫性.Ⅰ型鸭肝炎病毒属于小RNA病毒,Ⅱ型属于星状病毒,Ⅲ型为与Ⅰ型无关的另一种小RNA病毒.目前发生的鸭肝炎一般由Ⅰ型病毒引起,该病毒生命力较强,在自然环境下可在污染的孵化器和育雏室内存活10周以上,在阴凉处的湿粪中存活1月以上,在4℃冰箱内可存活2年,-20℃下则长达9年.因此,该病毒对鸭群危害较大,发病率较高.现将一例鸭病毒性肝炎的诊治过程报告如下.  相似文献   

6.
犬瘟热是由犬瘟热病毒引起的一种高度接触性传染病,该病毒是副粘病毒科、麻疹病毒属的成员。我们对2003年2~4月份共诊治的犬瘟热及疑似病例55例进行了分析,其发病情况多种多样,较为复杂。现报道如下,供参考。  相似文献   

7.
牛诺如病毒(BNoV)是国内新发的犊牛腹泻病病原,高度变异且传染性极强.牛感染该病毒后发病较为突然,能够引起腹泻呕吐等临床症状,一般情况下诺如病毒较为温和但是在暴发时感染率可达45%以上,甚至引起出血性的死亡,给养牛业造成经济损失.因此,建立一种特异性强、灵敏度高、耗时短的检测方法对准确检测BNoV及其流行病学调查等均具有重要意义.实时荧光定量PCR方法敏感而快捷,操作简便,因此荧光定量PCR技术被广泛应用于兽医学的检测.本研究设计了一对BNoV的特异性引物和探针,建立了TaqMan荧光定量PCR的检测方法,为快速检测和及早诊断BNoV提供有效的技术保障.  相似文献   

8.
猪伪狂犬病病毒(pseudorabies virus,PRV)是猪伪狂犬病的病原.目前,针对该病毒有较多成熟的商品化疫苗,但病毒变异频繁,因而在生猪养殖中伪狂犬病的发生仍然较为普遍.如何清除宿主体内野毒是防控该病的关键所在.应用腺联病毒携载CRISPR/Cas9系统,以小鼠为动物模型,针对PRV的TK基因、gE基因和V...  相似文献   

9.
正细小病毒作为一种宿主广泛的病毒,流行已将近一个世纪。而今,已成为侵染动物最频繁的病毒之一。第一例猫细小病毒(Feline parvovirus virus,FPV)是在20世纪初期由法国学者Verge发现,随后由Bilin和Johnson于1957年成功分离培养出该病毒~([1])。. FPV属于细小病毒科、细小病毒属成员,该属病毒是自然分布极广的一种微小的单股负链  相似文献   

10.
犬细小病毒(Canine Parvovirus,CPV)是1978年由Eugster首先从出血性肠炎患犬的粪便中发现的一种小DNA病毒.1982年,原解放军兽医大学在长春地区首次分离出该病毒,从而证实了我国也有犬细小病毒病存在.犬细小病毒是一种以急性出血性肠炎和非化脓性心肌炎为特征的一种高度接触性传染病.该病从发现至今,世界各地均有该病流行,该病已成为危害犬类的最主要的烈性传染病之一.  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

17.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

18.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

19.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

20.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

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