共查询到20条相似文献，搜索用时 46 毫秒
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Alberti A Robino P Chessa B Rosati S Addis MF Mercier P Mannelli A Cubeddu T Profiti M Bandino E Thiery R Pittau M 《Veterinary microbiology》2008,128(1-2):81-89
This paper reports the identification and characterisation of a 60kDa surface protein antigen (P60) of Mycoplasma capricolum subspecies capricolum (Mcc), and describes its diagnostic application. Genomic localization and presence in P60 of conserved functional domains suggested a structural and functional relationship with the immunodominant antigen P48 of Mycoplasma agalactiae, a basic membrane protein. A rP60-ELISA was developed, and it resulted in a high specificity for Mcc infections after evaluation with 125 goat sera. The comparison with an existent ELISA based on whole Mcc cell lysates showed that the two assays have comparable sensitivities, but the rP60-ELISA has the significant advantage of a greater specificity. Results indicate that P60 is a potential marker of Mcc infection, especially useful in areas where the presence of M. capricolum subspecies capripenumoniae is also reported. 相似文献
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旨在阐明山羊胚性腺的发生规律,这对于山羊的繁殖育种、丰富胚胎学有关研究内容都具有重要意义.该研究对22枚从39日胎龄胎儿至初生奶山羊性腺进行石蜡组织切片,用组织学和免疫组化、免疫荧光方法对山羊胎儿性腺发育的组织学特点,山羊胎儿性腺发育过程中VASA及细胞增殖核抗原(PCNA)的表达规律进行了系统研究.结果表明:奶山羊性腺在39～47日胎龄时已出现精索和白膜,已发生性别分化,并可分辨出生殖细胞、支持细胞及间质细胞,细胞增殖率较高.在大约50日胎龄前生殖细胞可称作性原细胞,细胞为球形、核质比高.在51～59日胎龄,生殖细胞发生剧烈变化:体积增大、核质比降低.此后逐渐由性原细胞发育为前精原细胞,并且在此期间表达VASA的生殖细胞增多.50～90日胎龄,生殖细胞增殖率最高,生殖细胞所占比例也达到峰值.在90日胎龄后生殖细胞增殖率下降,比例下降,间质细胞大量退化.结果表明,山羊胎儿的生殖细胞在50日胎龄后由性原细胞发育为前精原细胞,生殖细胞在50～90日胎龄的增殖率最高,VASA可作为雄性山羊胎儿前精原细胞的标记. 相似文献
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Zarankiewicz T Madej J Galli J Bajzert J Stefaniak T 《Polish journal of veterinary sciences》2012,15(2):373-378
Histophilus somni is an opportunistic pathogen causing respiratory, genitourinary and generalized infections in cattle. An important virulence factor is its ability to produce a biofilm. The aim of this work was to confirm that H. somni Hsp60 (Gro-EL) is a constituent of the biofilm produced by this bacterium in vitro and to check whether or not the presence of a specific antibody within the culture medium can inhibit biofilm production. Biofilm production by H. somni cultured in vitro was confirmed by crystalline violet staining. The presence of Hsp60 in the biofilm was confirmed by using specific antibodies produced in a mouse and goat hyperimmunized with H. somni recombinant Hsp60 (rHsp60). Large complexes of biofilm stained with Hsp60 antibodies were microscopically detected. This indicates that the Hsp60 protein is a common constituent of the biofilm produced by H. somni in vitro. In a second experiment, mouse serum containing anti-H. somni rHsp60 antibodies was added to an H. somni culture. It was found that the presence of anti-rHsp60 antibodies in the culture medium inhibited biofilm production in vitro. Only small biofilm particles were seen in the presence of the specific antibody, whereas in control cultures (without specific antiserum) large biofilm complexes were produced. The results indicate that antibodies specific to Hsp60 may be useful for preventing H. somni biofilm formation in vitro. If this also occurs in vivo, it may be helpful for eradicating H. somni infection in cattle through the elimination of carriers. Further in vivo studies are needed to confirm this idea. 相似文献
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用组织块培养法获得山羊乳腺细胞原代培养物。根据山羊乳腺成纤维细胞与上皮细胞对胰蛋白酶的敏感性不同将二者分离纯化，对细胞生长特性进行了光镜观察。结果如下：细胞可形成闭合型细胞群和开放型细胞群。乳腺上皮细胞与成纤维细胞混生时，细胞之间形成许多腔状结构。纯化的乳腺上皮细胞通过单细胞悬浮后传代，部分细胞形成岛屿状聚集，部分细胞以贴壁的自由单个细胞散在形式存在。上皮细胞增殖可形成圆顶型结构，呈乳头状，称之为乳球体；可产生乳腺上皮细胞并分泌乳汁。山羊乳腺上皮细胞含不同的细胞类型．大多数上皮细胞呈短梭形或多角形。细胞之间紧密相靠。互相衔接。连接成片，呈蜂窝状；细胞核呈圆形或椭圆形．核仁2～4枚；部分细胞呈圆饼状，体积较大；出现部分长形细胞；接触抑制的上皮细胞形态不均一。纯化的山羊乳腺上皮细胞传代至第15代时其生长仍正常，经透射电镜观察发现细胞表面微绒毛极为发达，细胞质中线粒体和粗面内质网丰富，细胞质内有大量脂滴及小泡，表明第15代山羊乳腺上皮细胞增殖活力旺盛。染色体数目分析表明．该细胞系稳定，在离体培养条件下细胞不发生转化。山羊乳腺上皮细胞系的细胞染色体数目为60．染色体组型为2n=60。 相似文献
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基于SNP芯片的云上黑山羊遗传结构分析 总被引：1，自引：0，他引：1
为从分子水平对云上黑山羊遗传结构做出评价,本研究以云上黑山羊核心群母羊108只(来自7个育种核心场、10个群体)、育种素材云岭黑山羊和努比山羊各11和14只、对照组波尔山羊10只为试验动物,用Illumina公司Iselect Goat60k芯片技术在全基因组范围内进行单核苷酸多态性(SNP)分析,并运用GenAlEx 6.5、Cervus 3.0、SNPRelate、Plink 1.07、Mega 4.0进行遗传多样性参数统计计算、主成分分析和系统进化树构建。试验获得143个样本在48 116个SNPs位点上的分型结果,其中波尔山羊10个样本单独聚集,与云上黑山羊、努比山羊、云岭黑山羊存在较大的遗传距离,较小的遗传一致度和较小的基因流,在聚类进化树中显示为一个独立分支,这些结果与波尔山羊实际遗传背景完全一致,显示出它在本研究中的对照组作用,同时证明本研究方法可行、结果可靠;云上黑山羊108个样本具有高度一致性,在聚类系统树中这些样本聚为一个大簇,而努比山羊(14个)和云岭黑山羊(11个)的样本各自聚为一簇;UPGMA进化树显示,云上黑山羊与努比山羊关系较近,与云岭黑山羊关系稍远,这与云上黑山羊育种导血方案结果相一致。云上黑山羊新品种在基因组水平上可与其育种素材明显区分,在基因组层面已具备独立品种特点。 相似文献
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CD81和CCL26基因是影响哺乳动物性腺细胞融合的重要因子,但其在黔北麻羊性腺组织中的表达情况尚不清楚。为研究CD81和CCL26基因在黔北麻羊不同组织中的表达量,本实验以单、多羔黔北麻羊为研究对象,提取下丘脑、垂体、子宫、输卵管、卵巢组织的RNA,并将5种性腺组织RNA逆转录合成第一链cDNA,随后采用q-PCR技术检测CD81、CCL26基因的mRNA在单、多羔黔北麻羊不同性腺组织中的表达水平。结果表明:黔北麻羊性腺组织中CD81、CCL26基因的mRNA均有表达,2种基因均在单、多羔卵巢中的表达量最高;CD81基因在单羔组子宫中的表达量最低;CD81基因在多羔组、CCL26基因在单多羔组下丘脑中的表达量均最低;组间差异表达量分析可知,CD81基因在多羔组子宫的表达量显著高于单羔组;CCL26基因在单羔组卵巢和输卵管的表达量显著高于多羔组,在单羔组子宫的表达量极显著高于多羔组。本实验结果提示,CD81和CCL26基因可能与山羊繁殖能力相关,也为初步揭示山羊繁殖的分子调控机制提供了参考依据。 相似文献
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Erik J Olson Scott R R Haskell Rodney K Frank Howard D Lehmkuhl Lea Ann Hobbs Janet V Warg John G Landgraf Arno Wünschmann 《Journal of veterinary diagnostic investigation》2004,16(5):461-464
A dairy goat operation in Minnesota experienced a sudden, markedly increased mortality among its neonatal goats. Approximately 60 of 130 kids (46%) died. The animals had diarrhea and dyspnea of 1-2 days duration before death. Necropsy of 4 goat kids revealed marked, acute, catarrhal enteritis and fibrinous pleuropneumonia. Mannheimia haemolytica was isolated from the lungs. Basophilic inclusion bodies filling the entire nucleus were present in enterocytes of the ileum of 3 goats. Adenoviral particles were detected in the feces by electron microscopy and adenovirus was subsequently isolated from the intestinal content together with a parvo-like virus (dependovirus). Morphology, physicochemical characteristics, and neutralization tests indicated that the adenovirus resembled ovine adenovirus-2 (OAdV-2). However, the PstI restriction endonuclease pattern produced by the goat adenovirus was distinct from that of OAdV-2. This is the first report of enteritis in goats with an adenovirus antigenically related to OAdV-2 and with a parvo-like dependovirus. 相似文献
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Maria Excelsis M. ORDEN Wilfred E. JAMANDRE Ernesto O. BROWN Edgar A. ORDEN Emilio M. CRUZ Ana Marie P. ALO Edwin C. VILLAR 《Animal Science Journal》2005,76(2):179-185
Preference for goat characteristics was determined from 42 traders in six markets in Pangasinan, an important trading center for goats in Luzon, the Philippines, and analyzed using a hedonic price model to determine whether these characteristics are important price determinants of goats. This information is important to goat raisers to respond to the needs of the local market, and thus to achieve a better price and higher income in goat raising. Meatiness, size, breed, sex and age (in descending order) were the dominant goat characteristics preferred by traders. Meatiness had the largest positive influence on price, with a price premium of US$6.24 (US$1.00 = PhP 53), whereas the age of the animal had the smallest influence at US$3.72. However, size and meatiness are manifestations of good breeds. Therefore, continued efforts to improve breeds in order to produce good quality goats are imperative to cater to market needs. Different characteristics implied different price premiums. Traders offered a higher price premium for better goat characteristics; thus, the improvement of these characteristics could result in higher returns for goat raisers. 相似文献
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山羊生长激素基因部分序列的PCR-SSCP分析 总被引：5，自引：0，他引：5
以鲁北白山羊、波尔山羊和波尔山羊与鲁北白山羊的杂交一代、回交一代共计 2 74个个体为研究材料 ,对山羊生长激素基因 5′调控区 - 4 0 6～ - 1 93片段进行PCR扩增 ,扩增产物经SSCP分析发现存在多态性。结果表明发现波尔山羊及杂交后代以AA型占多数 ,而鲁北白山羊则BB型个体较多 ,基因型频率在品种间存在显著差异 (P <0 .0 5 )。对该片段的两种纯合型克隆测序发现 :AA型在第 6 0位发生了C→T的突变 ,第 2 1 1位发生碱基C的丢失 相似文献
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Yokota S Matsuu A Morita T Tsuka T Hishinuma M Minami S Hikasa Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1355-1357
A 5-day-old hornless goat was referred with dysuria since birth. The scrotum was absent, and a small penis-like structure was seen below the perineal raphe. On the laparotomy, the testicles were found near the inguinal ring- and attached to a uterus-like structure. On histological analysis, the uterus-like structure was blind-end. Germ cells were absent in the testis. The karyotype of this goat was 60, XX and the SRY gene was absent. The goat was homozygous for a DNA deletion responsible for the Polled Intersex Syndrome (PIS). To the authors' knowledge, this is the first report as the clinical case of the PIS-/- goat with urethral atresia. 相似文献
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Although research on dairy goat mammary gland have referred extensively to molecular mechanisms, research on lines of dairy goat mammary epithelial cells (MECs) are still rare. This paper sought to establish an immortal MEC line by stable transfection of human telomerase. MECs from a lactating (45 days post‐parturition) Xinong Saanen dairy goat were cultured purely and subsequently transfected with a plasmid carrying the sequence of human telomerase. Immortalized MECs by human telomerase (hT‐MECs) exhibited a typical cobblestone morphology and activity and expression levels of telomerase resembled that of MCF‐7 cells. hT‐MECs on passage 42 grew vigorously and ‘S’ sigmoid curves of growth were observed. Moreover, hT‐MECs maintained a normal chromosome modal number of 2n = 60, keratin 8 and epithelial membrane antigen (EMA) were evidently expressed, and beta‐casein protein was synthesized and secreted. Beta‐casein expression was enhanced by prolactin (P < 0.05). Lipid droplets were found in hT‐MECs, and messenger RNA levels of PPARG, SREBP, FASN, ACC and SCD in hT‐MECs (passage 40) were similar to MECs (passage 7). In conclusion, the obtained hT‐MEC line retained a normal morphology, growth characteristics, cytogenetics and secretory characteristics as primary MECs. Hence, it can be a representative model cell line, for molecular and functional analysis, of dairy goat MECs for an extended period of time. 相似文献
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针对云南夏秋高温高湿气候特点，按照国家环境监测规范，对山羊舍内环境进行监测，结果表明：双列式羊舍舍内温度、湿度、气流速度、采光效果等物理卫生指标因舍内羊床的方位不同而变化；在每只山羊占有舍内空间10．4m^3的条件下，6月份上述物理卫生指标基本符合山羊生长发育的要求；舍内总悬浮颗粒物0．15mg／m^3；可吸入颗粒物0．059mg／m^3；氨气0．32mg／m^3；硫化氢0．0031mg／m^3；恶臭66，与国家畜禽场空气环境质量标准相比，有害气体浓度较低，适合山羊舍饲要求。 相似文献
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Takeyuki OZAWA Kumiko MUKUDA Masaru FUJITA Jiro NISHITANI 《Animal Science Journal》2009,80(2):212-219
A consumer questionnaire conducted with the purpose of ascertaining the acceptability of goat milk and related products in Japan was carried out on 345 guarantees of Nippon Veterinary and Life Science University in December 2006. 275 effective responses (79%) representing middle class urban households were returned. The results revealed that (1) 30% of respondents have experienced drinking goat milk and only 10% are aware of the current retail situation of goat milk and related products; (2) over 70% of goat milk drinkers raised goats by hand at some point in their past and their first experience drinking goat milk was in infancy; (3) those with experience in drinking goat milk expressed a vague evaluation and minimal understanding of drinking goat milk; (4) respondents who were inexperienced goat milk drinkers expressed a strong desire to taste and a weak desire to purchase goat milk; (5) respondents expressed low recognition regarding retailed goat milk products, but those who had already purchased goat milk products expressed a high evaluation and strong desire to purchase these products again; and (6) recognition of goat milk characteristics is low, but those with high recognition also rate goat milk highly. Goats are perceived as being 'mild and familiar.' It is necessary for those who manage goat husbandry to present goat milk and related product tasting opportunities to consumers. The key point is to make the functional differences between cow and goat milk clear and present the advantages of goat milk at the fore of this promotion. Goat milk should not be promoted merely as a drink that is similar to cow milk, but must be positioned as a functional drink or health food in order to expand the Japanese goat milk market. 相似文献
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Orsel K van Amerongen JJ Zadoks RN van Doorn DC Wensing T 《Tijdschrift voor diergeneeskunde》2000,125(23):709-712
In this study, which was performed on a Dutch dairy goat farm, several aspects of the administration of colostrum to new-born goat kids were examined. Time of colostrum administration and amount and type of colostrum administered were compared. Effectiveness was measured as total serum protein content and gamma globulin fraction. No significant differences in serum gamma globulin titre were observed between kids that received colostrum at 30 or 60-90 minutes post partum, respectively. Titres were significantly lower in kids that received 100 ml of colostrum instead of 150-200 ml. The effect of sheep colostrum replacer or cow colostrum was also examined. Gamma globulin titres were significantly high with goat colostrum than with cow colostrum or sheep colostrum replacer, and titres were higher with cow colostrum than with sheep colostrum replacer. Based on the results of this experiment, the following protocol is suggested for colostrum administration to goat kids: single administration of 150-200 ml of goat colostrum within 90 minutes of birth. Use of cow colostrum is not advised because it may lead to transmission of paratuberculosis. Use of sheep colostrum replacer as a source of passive immunity is not recommended. 相似文献
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Seroprevalence of, and risk factors for, peste des petits ruminants in sheep and goats in Northern Jordan 总被引：1，自引：0，他引：1
Peste des petits ruminants (PPR) is an economically important disease that affect sheep and goat industry in Asia and Africa. In this study, we investigated the seroprevalence, and risk factors, of PPR in sheep and goat flocks from five different governorates (Irbid, Jarash, Ajloun, Mafraq and Zarka) located in Northern Jordan. Serum samples from 929 and 400 sheep and goats, respectively, corresponding to 122 sheep flock and 60 goats flock were collected. Seroprevalence was determined using PPR competitive ELISA. Health status and management information were collected using a semi-structured pre-tested questionnaire. The individual true prevalence of PPR in sheep and goats was 29 and 49%, respectively. The flock level true prevalence of PPR was 60 and 74% in sheep and goats, respectively. In both sheep and goat flocks, large flock size, visiting live animals market and inadequate veterinary services were identified as risk factors for PPR seropositivity. Mixed (sheep and goats) raising was identified as a risk factor for PPR seropositivity in sheep flocks only. 相似文献
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HUA Liu-shuai WANG Jing CHEN Fu-ying XIN Xiao-ling CHU Qiu-xia FENG Ya-jie XU Zhao-xue WANG Er-yao 《中国畜牧兽医》2017,44(9):2566-2572
This study was aimed to constract growth differentiation factor 9 (GDF9) lentiviral vector,which was stably expressed in goat primary fibroblast. The CDS of sheep GDF9 gene was cloned by gene synthesis, the CDS was 1 362 bp, encoding 453 amino acids. After double enzyme digestion and ligation, the GDF9 fragment was sub-cloned into an empty lentiviral vector. The GDF9 lentiviral vector and the packaging plasmids were co-transfected into 293T cells, and the lentivirus with titer of 1×106 TU/mL was obtained. The goat primary fibroblasts were transfected with GDF9 lentivirus, the red fluorescence could be observed in more than 60% of the cells, suggesting the prepared lentivirus had high infection efficiency to goat primary fibroblasts. After puromycin screening, all cells were able to observe red fluorescence. Real-time quantitative PCR analysis showed that the GDF9 expression level was higher than that of control group. The results indicated that the goat primary fibroblasts stably expressing GDF9 were successfully obtained. This results might lay a foundation for the function study of GDF9 and the goat germplasm resources innovation in the future. 相似文献