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1.
The ultrastructure of porcine circovirus was examined in persistently infected porcine kidney (PK)-15 cells. Virus-infected PK-15 cells had large numbers of intracytoplasmic inclusions, and a few cells had intranuclear inclusions. Intracytoplasmic inclusions were dispersed throughout the cytoplasm but were most numerous in the perinuclear cytoplasm. Inclusion were of various sizes, round to oval, and electron dense and were of two general types. Inclusions of the first type were small (0.1-0.5 microm diameter), not surrounded by trilaminar membranes, and granular with indistinct margins that blended with surrounding cytoplasm. Some contained 12+/-2-nm-diameter icosahedral virions in loose aggregates or rarely forming paracrystalline arrays. Small inclusions could be sites of viral assembly or maturation. Intracytoplasmic inclusions of the second type were larger (0.5-5.0 microm diameter) and more numerous and had abrupt margins surrounded by trilaminar membranes. They were more electron dense than small inclusions and were heterogeneous, containing various proportions of aggregated virions, electron-dense crystalline lamellae of 5 nm periodicity, and/or whorls of myelinoid membranes. Virions usually formed paracrystalline arrays and occasionally were loosely aggregated. Larger inclusions were typical of autophagolysosomes. Intranuclear inclusions were not membrane bound and were often associated with reticulated nucleoli or aggregates of heterochromatin. Some inclusions were irregularly shaped aggregates of indistinct, circular 10-12-nm-diameter viruslike particles. Others were 0.1-1.0 microm in diameter, round or ring shaped, dense, and finely granular, with sharply demarcated margins. 相似文献
2.
兔出血症病毒在细胞培养和组织中的形态发生 总被引：4，自引：2，他引：2
在电镜下系统地观察了感染后的细胞培养和组织中兔出血症病毒（ Ｒ Ｈ Ｄ Ｖ）的形态发生。感染早期，在细胞核内可见电子致密颗粒（１５ ｎｍ ）和未成熟的病毒颗粒 （２５ ｎｍ ）。中期，在细胞核和胞浆内出现大量成熟的病毒颗粒（３４ ｎｍ ），并发现部分核内病毒通过扩大的核膜孔、核膜溶解扩大的核孔和乳头状突起的核膜向胞浆释放。感染末期，核染色质消失，核内大量感染病毒清淅可见。最终细胞溶解，病毒颗粒释放至细胞间隙。提示 Ｒ Ｈ Ｄ Ｖ 是在核内复制和装配的，应归属于细小病毒科。本试验结果不排除同时存在另一种小 Ｒ Ｎ Ａ 病毒。 相似文献
3.
Chicken embryos and healthy adult chickens naturally infected with lymphoid leukosis virus were used to investigate viral inclusion bodies in myocardial cells by light and electron microscopies and by immunocytochemical technique. Intracytoplasmic viral matrix inclusion bodies frequently appeared in the myocardium of adult chickens, but not in that of embryos. In light microscopic preparations, inclusions were irregularly distributed, were basophilic, and contained ribonucleic acid. Ultrastructurally, inclusions in myocardial cells were in areas containing numerous interstitial C-type particles. Early inclusions were composed of clusters of ribosomes associated with sarcoplasmic tubules; spherical bodies developed among these ribosomes. Mature inclusions were composed of numerous spherical bodies (50 to 75 nm) with interspersed ribosomes and of ribosomes clustered at the periphery. Inclusions were not membrane-enclosed. Occasionally, spherical bodies were in paracrystalline arrays. Multiple budding occurred on cell membranes adjacent to matrix inclusions. The viral group-specific protein, p27, was demonstrated by the peroxidase-antiperoxidase method and by the protein A-gold method in the spherical bodies, in nucleoids of mature virus particles, and among ribosomes of inclusions. The results indicate that the matrix inclusions were the result of lymphoid leukosis virus infection and were the product of viral protein synthesis on ribosomes. 相似文献
4.
Nakamura K Waseda K Yamamoto Y Yamada M Nakazawa M Hata E Terazaki T Enya A Imada T Imai K 《Avian diseases》2006,50(1):152-156
Cutaneous fowlpox occurring in vaccinated layer hens was investigated pathologically and microbiologically. Anorexia, decrease of egg production, increased mortality, yellow scabs on faces, and alopecia of feathered skins with yellow scabs were observed in affected hens. Histologically, proliferative and necrotic dermatitis with eosinophilic ring-shaped cytoplasmic inclusions (Bollinger bodies) and clumps of gram-positive cocci (Staphylococcus hyicus) were noted in the affected birds. Fowlpox lesions were primarily observed in the feathered skins. Proliferation of feather follicle epidermal cells, with cytoplasmic inclusions and degeneration of the feather, and bacterial clumps in the feather follicles were noted in the affected skins. Ultrastructurally, characteristic fowlpox viral particles were observed in the cytoplasmic inclusions of hyperplastic epidermal cells. Amyloid deposition was observed in the Disse space of the liver, splenic sinus, and lamina propria of the bronchiolar, bronchial, and tracheal areas. Amyloidosis could be one factor inducing the fowlpox infection in vaccinated chickens. 相似文献
5.
Young lambs, inoculated with ovine adenovirus isolate RTS-151, were killed sequentially (to postinoculation day 21), and their morphologic lesions were studied. Lesions were found only in the respiratory tract and were most severe in the terminal airways. Many cells of the respiratory epithelium had severe cytomegaly and karyomegaly with intranuclear inclusions. Desquamation of affected cells was accompanied by hyperplasia of airway and alveolar epithelium and by accumulation of necrotic cellular debris and neutrophils in terminal airways and alveoli. Virus replicated in the nucleus of enlarged epithelial cells in the airways, but not in alveolar epithelium. Intranuclear inclusions representing viral replication progressed from clumps of finely granular material without virions to a granular material with virions and finally to a dense inclusion with numerous virions. 相似文献
6.
Conjunctivitis, tracheitis, and pneumonia associated with herpesvirus infection in green sea turtles 总被引：3，自引：0，他引：3
E R Jacobson J M Gaskin M Roelke E C Greiner J Allen 《Journal of the American Veterinary Medical Association》1986,189(9):1020-1023
Fourteen juvenile (15- to 20-month-old) green sea turtles (Chelonia mydas), representative of a group of sea turtles with clinical signs of respiratory tract disease, were euthanatized and submitted for necropsy. Macroscopically, lesions included periglottal necrosis, tracheitis with intraluminal caseous and laminated necrotic debris, and severe pneumonia. Several turtles had caseous conjunctival exudate covering the eyes. Microscopically, the turtles had fibrinonecrotic inflammation around the glottal opening, tracheitis, and severe bronchopneumonia and interstitial pneumonia. In multifocal areas, periglottal and tracheal epithelial cells adjacent to areas of necrosis had hypertrophic nuclei with amphophilic intranuclear inclusions. A mixed population of primarily gram-negative microorganisms was isolated from the tracheal and glottal lesions. Attempts at viral isolation in cultures of green sea turtle kidney cells resulted in the development of cytopathic effects characterized by giant cell formation and development of intranuclear inclusions. Using electron microscopy, intranuclear viral particles (88 to 99 nm in diameter) were seen in inclusion-containing tracheal and glottal epithelial cells and infected green sea turtle kidney cells; particles were consistently seen enveloping from nuclear membranes, and mature particles (132 to 147 nm) were found in the cytoplasm. On the basis of size, conformation, location, and presence of an envelope, the particles most closely resembled those of herpes-viruses. 相似文献
7.
M Hamada T Oyamada H Yoshikawa T Yoshikawa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(2):345-351
Esophageal papillomas naturally occurring in 35 cattle were examined by light and electron microscopy. The characteristic histological lesions noted were acanthosis and parakeratosis of the mucous membrane with formation of basophilic intranuclear inclusion bodies in cells of the stratum corneum. Electron microscopically, viral particles were observed in the strata spinosum and corneum, and larger aggregations of viral particles appeared to correspond to the histological inclusions. The virus particles had a hexagonal profile, measured 35-40 nm in diameter and were arranged in crystalline arrays in some nuclei. The viral particles had morphologic characteristics indistinguishable from those of other papilloma viruses. The inclusions were stained positively by immunohistochemical methods using anti-bovine papilloma virus serum. 相似文献
8.
jell Andersen 《Acta veterinaria Scandinavica》1978,19(2):229
The ultrastructural features, characterizing the different types of spermatogonia and spermatocytes in the blue fox, have been studied within and near the reproductive season, and also in the summer and autumn.Two distinct types of spermatogonia — A and Β — are described. The A-spermatogonia often have a prominent nucleolus and numerous cytoplasmic organelles including characteristic whorls of AER. Large vacuoles containing electron dense particles are sometimes observed. In the B-spermatogonia the chromatin forms condensed areas of varying size, and the nucleolus is usually absent. The number of cytoplasmic organelles is generally small.Ultrastructural characteristics are further used to distinguish between the different stages in the prophase of the primary spermatocytes. In leptotene the nucleus contains a thread-like chromatin with electron dense peripheral areas. Towards the end of the stage the mitochondria display dilated cristae, and aggregations of a granular material can be observed in the intermitochondrial matrix. Zytogene is characterized by the appearance of syniaptinemal complexes in the nucleus, and of the chromatoid body and piles of annulate lamellae in the juxtanuclear cytoplasm. In pachytene the chromosomes become apparent as aggregations of condensed chromatin associated with the synaptinemal complexes. The Golgi complex is more prominent than in the previous stages, and the number of the other cytoplasmic organelles is increasing. In the last stages of the prophase (diplotene and diakenesis) the chromosomes become still more electron dense, the nucleolus appears as a very prominent structure, and there is a marked vesiculation of the cytoplasm.The secondary spermatocytes have a characteristic nucleus with a somewhat irregular outline and larger peripheral areas of condensed chromatin. In the cytoplasm a double Golgi complex is frequently observed.In the summer and autumn spermatocytes in zygotene seem to represent the most advanced form of spermatogenic cells. 相似文献
9.
Kiatipattanasakul-Banlunara W Tantileartcharoen R Katayama K Suzuki K Lekdumrogsak T Nakayama H Doi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):527-529
Three sulphur-crested cockatoos (Cacatua galerita) were diagnosed as psittacine beak and feather disease (PBFD). Histopathology of the feather pulp and follicles showed intracytoplasmic botryoid clusters or granular inclusion bodies in epithelial cells and macrophages. Electron microscopy revealed multiple cytoplasmic clusters of electron dense viral particles corresponding to the inclusions. PBFD virus (circovirus) DNA-specific product was detected from formalin-fixed paraffin-embedded feathers by nested polymerase chain reaction (PCR) method. 相似文献
10.
A female bearded dragon (Amphibolurus barbatus) died following intermittent periods of inappetance. No significant gross lesions were found at autopsy, but histological examination revealed disordered liver architecture with numerous foci of coagulative necrosis. Eosinophilic intranuclear inclusions were present in many hepatocytes, some epithelial cells of the bile ductules and occasional epithelial cells of renal tubes and glomeruli. Large numbers of viral particles within many nuclei, associated with the intranuclear inclusion were demonstrated by electronmicroscopy. Similar particles, sometimes in paracrystalline arrays, were also seen within membrane-bound vesicles located next to the nuclei and to a lesser degree within the cytoplasm and extracellular spaces. The virus was considered to be an adenovirus on the basis of its size, morphology, site of formation and lack of envelopment. It was considered to he the cause of the hepatitis. 相似文献
11.
鹅胸腺APUD(样)细胞的超微结构 总被引：2，自引：0，他引：2
应用透射电镜在鹅胸腺内发现一种ＡＰＵＤ（样）细胞。这种细胞形态不规则,有突起伸入相邻细胞之间;胞核圆形,电子密度低,核仁明显;胞质内含有粗面内质网、线粒体等丰富的细胞器。其主要特征是含有大量的多分布于胞质一侧的膜包小分泌颗粒,呈圆形或椭圆形,大小不等,直径１１０～５５０ｎｍ,有些颗粒在界膜与内含物之间可见低电子密度的晕轮。根据其超微结构特征,可将颗粒分为３型：Ⅰ型颗粒为中等电子密度;Ⅱ型颗粒为均质状高电子密度;Ⅲ型颗粒在中等电子密度中含有一高电子密度的核芯。作者认为这种细胞属于内分泌细胞,并且很可能是分泌肽类激素的ＡＰＵＤ细胞。 相似文献
12.
Sato K Shibahara T Ishikawa Y Kondo H Kubo M Kadota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(6):627-633
An epizootiological survey with histopathological methods was conducted for porcine circovirus in 220 diseased pigs (1-200 days old) in 49 farms from 1985 to 1999. Histopathological lesions containing PCV antigen were detected mainly in the lymphoid tissues from 42 of 189 diseased pigs (22.2%) in 4 of 45 farms (8.9%) from 1990 to 1999. The rate of positive pigs gradually increased from 1997 onward and PCV infection was found in 50% of diseased pigs in 1999. Histopathologically, the lesions in the lymphoid tissues (including lymph nodes, Peyer's patches, tonsil and spleen) were highly correlated with the presence of numerous spherical basophilic intracytoplasmic inclusion bodies with PCV antigen, and consisted of lymphocellular depletion and infiltration of macrophages. Although most affected cells showed cytoplasmic reactivity for PCV, intranuclear antigen was also seen in the lymphocytes, macrophages and ileal epithelial cells. Ultrastructurally, macrophages and giant cells contained electron-dense, round to ovoid lysosomal bodies, in which there were concentric circle or paracrystalline arrays of small nonenveloped icosahedral viral particles, approximately 15-17 nm in diameter. Other consistent infectious agents were present in 90.5% of cases, and porcine reproductive and respiratory syndrome virus infection was in 52.4% of the cases with PCV. The histopathological findings suggested that PCV induced systemic immunosuppression in the infected pigs and made them more susceptible to infection of the organisms. Because of the presence of PCV antigens in the intestinal epithelium, feces may play a significant role in dissemination of PCV. 相似文献
13.
A female bearded dragon (Atnphibolurus barbutus) died following intermittent periods of inappetance. No significant gross lesions were found at autopsy, but histological examination revealed disordered liver architecture with numerous foci of coagulative necrosis. Eosinophilic intranuclear inclusions were present in many hepatocytes, some epithelial cells of the bile ductules and occasional epithelial cells of renal tubes and glomeruli. Large numbers of viral particles within many nuclei, associated with the intranuclear inclusion were demonstrated by electronmicroscopy. Similar particles, sometimes in paracrystalline arrays, were also seen within membrane-bound vesicles located next to the nuclei and to a lesser degree within the cytoplasm and extracellular spaces. The virus was considered to be an adenovirus on the basis of its size, morphology, site of formation and lack of envelopment. It was considered to he tlie cause of the hepatitis. 相似文献
14.
Acute hepatitis in a piglet experimentally inoculated with tissue homogenates from pigs with postweaning multisystemic wasting syndrome 总被引：3，自引：0，他引：3
Hirai T Nunoya T Ihara T Kusanagi K Kato T Shibuya K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):1041-1045
Five 2 day-old colostrum-deprived piglets were inoculated with tissue homogenates from pigs with postweaning multisystemic wasting syndrome. One of the five piglets developed icterus and died 23 days post-inoculation. Histologic examination revealed acute hepatitis. Porcine circovirus type 2 (PCV-2) antigen and nucleic acid were detected in hepatocytes and phagocytic cells. Ultrastructurally, hepatocytes and phagocytic cells had large numbers of cytoplasmic inclusions, which were composed of electron-dense paracrystalline arrays of small non-enveloped viral particles approximately 17 nm in diameter. Apoptotic hepatocytes were confirmed by the TUNEL method and electron microscopic examination. These findings may indicate that hepatocellular necrosis is associated with replication of PCV-2. Apoptosis of hepatocytes also contributes to the pathogenesis of hepatic lesions in this case. 相似文献
15.
A three week old Holstein calf that died after a period of anorexia and diarrhea had a severe necrotizing ileitis. The lesions which were most severe above Peyer's patches contained numerous filamentous bacteria. Prominent intranuclear inclusion bodies in vascular endothelial cells in both the affected intestine and the mesenteric lymph nodes were accompanied by vasculitis and thrombosis. Adenovirus-like particles were demonstrated ultrastructurally in the inclusions.
This case demonstrates a preferential association of the intestinal necrosis with the gut associated lymphoid tissue. Also, the significance of viral infection of vascular endothelium versus intestinal epithelium in determining the type and severity of injury, is deduced from this and other previously reported cases.
相似文献16.
17.
Ono M Okuda Y Yazawa S Shibata I Tanimura N Kimura K Haritani M Mase M Sato S 《Avian diseases》2001,45(1):268-275
Two outbreaks of gizzard erosion in slaughtered broiler chickens in Japan were examined pathologically and microbiologically. The prevalences of such lesions were 9%-11% and 4%-50% in the affected flocks. Affected chickens had no clinical signs. Group I fowl adenovirus (FAV) serotype 1 was isolated from gizzard lesions. Histologically, gizzard mucosa were necrotic. Intranuclear inclusion bodies were seen in the enlarged nuclei of degenerating epithelial cells of the gizzard. The keratinoid layer in the erosion was edematous and desquamated and contained degenerative cells. Moderate to marked inflammatory cell infiltration was observed in the lamina propria and perivascular connective tissue in the submucosa and muscle layer. Immunohistochemical staining showed evidence of FAV antigens in the intranuclear inclusion bodies within degenerating epithelial cells. Ultrastructurally, numerous viral particles were demonstrated in the inclusions. 相似文献
18.
Electron microscopic findings of cells with inclusion bodies in experimental hemorrhagic enteritis of turkeys. 总被引：1，自引：1，他引：0 
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下载免费PDF全文 The spleen, liver, bone marrow and intestines of two turkeys in which hemorrhagic enteritis of turkeys was experimentally reproduced were examined electron-microscopically. Intranuclear inclusion bodies as described in a previous report were found in all the tissues examined. These occupied most of the area in affected nuclei and were composed of viral particles with morphological characteristics of an adenovirus. The cells with the inclusions were divided into two types of cells, immature and reticular cells. There was some variety in the stage of differentiation of the former cells. As the viral particles developed the cells degenerated and disintegrated. A few particles had been released into the cytoplasm of the degenerated cells but no particles were present in intercellular spaces. 相似文献
19.
Parvovirus-like enteropathy in Missouri turkeys 总被引：1，自引：0，他引：1
A viral enteric disease of young turkeys characterized by stunting of affected birds, diarrhea, and increased mortality is described. Eosinophilic intranuclear inclusion bodies were found in the absorptive epithelial cells of the ileum. Electron microscopy of formalin-fixed tissue revealed that the intestinal inclusions contained numerous loosely packed 15-to 20-nm hexagonal particles. The size, shape, and intranuclear location have been used to tentatively identify these particles as parvoviruses. 相似文献
20.
Homogenized lung tissue was used to experimentally reproduce lethal viral pneumonia in guinea pigs. The resultant lesions corresponded with those of the spontaneous disease. Pneumonia with necrotic bronchiolar epithelium accompanied by basophilic intranuclear inclusion bodies was the primary finding. With transmission electron microscopy, numerous viral particles were found--mainly within the nucleus of pulmonary epithelial cells but also within the cytoplasm and in extracellular space. The appearance of viral particles, in particular their paracrystalline and crystalline deposition within the nucleus, indicates that our experimentally induced pneumonia was caused by an adenovirus. 相似文献