共查询到18条相似文献，搜索用时 156 毫秒
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本文概述了微卫星DNA进行亲子鉴定的基本原理方法，将其用于亲子鉴定的优点以及研究进展，最后对未来的应用进行了展望。 相似文献
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概述了微卫星 DNA用于亲子鉴定的优点及存在的缺陷 ,并对未来的应用前景作了展望 相似文献
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DNA fingerprinting with the probes 33.15 and alpha-globin 3'HVR has been used to resolve three cases of disputed paternity in dogs. For each pedigree it was necessary to establish which bands in the DNA fingerprints of the offspring were of paternal origin, and then establish which putative sire carried all these bands. In the first case, a litter of Rhodesian Ridgebacks, twelve DNA bands were informative in establishing paternity. In the second case, a litter of Afghan hounds, five DNA bands established paternity, Lastly, in a litter of Border collies, five DNA bands established paternity. In each case a single dog only sired the entire litter. 相似文献
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12 cattle microsatellite DNA markers were used for establishing the paternity of 4 Datong Yak calves.The results indicated that paternity can be assigned by use of exclusion method directely when the mother is clearly known and data of all likely sires were obtained. The more the alleles of a locus possessed, the more effectively the paternity can be assigned. Not only the microsatellite DNA marker is a promising tool in establishment of yak detail pedigrees, but also can provide us information of reproductive efficiency of the bull(s) based on the numbers of calves it produced in the herd, which will be useful in speeding up the processes of yak genetics improvement. 相似文献
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Gomez-Raya L Priest K Rauw WM Okomo-Adhiambo M Thain D Bruce B Rink A Torell R Grellman L Narayanan R Beattie CW 《Journal of animal science》2008,86(1):17-24
The feasibility and economic value of DNA paternity identification were investigated and illustrated using Nevada beef cattle operations. A panel of 15 microsatellites was genotyped in 2,196 animals from 8 ranches with a total of 31,571 genotypes. Probabilities of exclusion for each marker within ranch and across ranches were computed. Joint probabilities of exclusion for the 15 microsatellites were also determined, resulting in values over 0.99 for any individual ranch and across ranches. Dropping 1 or 2 microsatellites with the lowest probabilities of exclusion resulted in joint probabilities greater than 0.99 and with marginal reduction compared with the probabilities with 15 microsatellites. Formulas for benefit-cost analysis for a DNA paternity identification program in beef cattle were derived. Genotyping 15 microsatellites with 20 calves per sire resulted in benefits of $1.71 and $2.44 per dollar invested at bull culling rates of 0.20 and 0.30, respectively. The breakpoints for the program to be profitable occurred when the ratio of the price of 1 kg of calf liveweight over the cost of genotyping 1 microsatellite was greater than 1.1 for a bull culling rate of 0.30. Benefit-cost analysis was also derived under incomplete DNA paternity identification using a lower number of DNA markers than necessary to achieve joint probabilities of exclusion of 0.99. Approximately a 20% increase in the benefit-cost ratio was achieved using 10 vs. 12 microsatellites with incomplete paternity identification. The greater the number of bulls in the operation, the lower the benefit-cost ratio of the paternity testing program. Low probabilities of exclusion and a high number of bulls in the beef operation reduced the benefit-cost ratio dramatically. The DNA paternity identification programs are feasible and may be profitable for free-range beef cattle operations. 相似文献
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微卫星DNA在牦牛亲权鉴定中的应用研究 总被引：1，自引：0，他引：1
为了探讨微卫星DNA在牦牛亲权鉴定应用的可行性,选用FAO推荐的17个微卫星座位,检测其在天祝白牦牛群体中的多态性水平,计算这17个微卫星座位用于亲权鉴定的累积排除率。结果表明,17个微卫星座位平均等位基因数为6.235;多态信息含量介于0.191~0.824之间,平均为0.608,为高度多态;一个候选亲本的累积排除概率为0.997,2个候选亲本的累积排除概率为0.999 999 97,各个微卫星座位零等位基因频率均在0.05以下,微卫星DNA可以应用于牦牛的亲权鉴定。 相似文献
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M. M. Binns N. G. Holmes E. Marti N. Bowen 《The Journal of small animal practice》1995,36(11):493-497
Canine microsatellites, comprising one to four base pair repeated sequences, were identified as part of a project to generate a genetic linkage map of the dog. They have been used to assign parentage in a range of doubtful paternity cases, for example, all the pups in a litter from a bitch mated on different days to two different stud dogs were identified by DNA analysis to come from one dog only. DNA typing in another case was able to exclude a male dog as the father of a litter. DNA analysis can be of relevance by allowing pedigree dogs, which would have previously been excluded on the basis of unknown paternity, to be registered with the appropriate kennel club. 相似文献
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A case of disputed paternity in dogs is reported. DNA examinations were carried out from hair samples of the individuals several months after the death of the putative sire. Ten short tandem repeat (STR) loci were analysed by fluorescence-labelled multiplex PCR using ABI PRISM 310 Genetic Analyser. Based on the results the candidate sire was included in the pedigree records as the biological sire. In spite of the genetic homogeneity of pedigree dogs due to inbreeding, canine microsatellites can provide an adequate basis for assigning paternity in pure breeds. 相似文献
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DNA fingerprinting was used to study the population genetics and paternity of the Rasa Aragonesa sheep (Ovis aries). Using a combination of Hae III and the M13 derived probe pV47, the mean number of bands per individual (5.422 +/- 0.309), the background band sharing coefficient (0.347), the mean population frequency of alleles (0.192) and the mean heterozygosity for bands (0.893) were calculated for 45 individuals from eight different farms in Aragon, northern Spain. Therefore, the estimated probability of missing a wrong paternity was 0.126 for unrelated males, and this probability was 0.438 for full-sibs males. In addition, in a field trial of this technique, paternity was assigned for three ewe-lamb pairs with a probability of 97 per cent in two cases and 77 per cent in one case. 相似文献