共查询到20条相似文献，搜索用时 28 毫秒
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K DYNON A VARRASSO N FICORILLI SA HOLLOWAY GH REUBEL F LI CA HARTLEY MJ STUDDERT HE DRUMMER 《Australian veterinary journal》2001,79(10):695-702
OBJECTIVE: To develop rapid (< 8 hour) tests using polymerase chain reaction (PCR) for the diagnosis of equine herpesvirus 3 (EHV3; equine coital exanthema virus), equine gammaherpesviruses 2 (EHV2) and EHV5, equine adenovirus 1 (EAdV1), EAdV2, equine arteritis virus (EAV), equine rhinitis A virus (ERAV; formerly equine rhinovirus 1) DESIGN: Either single round or second round (seminested) PCRs were developed and validated. METHODS: Oligonucleotide primers were designed that were specific for each virus, PCR conditions were defined and the specificity and sensitivity of the assays were determined. The application of the tests was validated using a number of independent virus isolates for most of the viruses studied. The PCRs were applied directly to clinical samples where samples were available. RESULTS: We developed a single round PCR for the diagnosis of EHV3, a seminested PCR for EHV2 and single round PCRs for EHV5, EAdV1, EAdV2 and RT-PCRs for EAV and ERAV. The PCR primer sets for each virus were designed and shown to be highly specific (did not amplify any recognised non-target template) and sensitive (detection of minimal amounts of virus) and, where multiple virus isolates were available all isolates were detected. CONCLUSION: The development and validation of a comprehensive panel of PCR diagnostic tests, predominantly for viruses causing equine respiratory disease, that can be completed within 8 hours from receipt of clinical samples, provides a major advance in the rapid diagnosis or exclusion diagnosis of these endemic equine virus diseases in Australia. 相似文献
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Holmes MA Townsend HG Kohler AK Hussey S Breathnach C Barnett C Holland R Lunn DP 《Veterinary immunology and immunopathology》2006,111(1-2):67-80
Horses are commonly vaccinated to protect against pathogens which are responsible for diseases which are endemic within the general horse population, such as equine influenza virus (EIV) and equine herpesvirus-1 (EHV-1), and against a variety of diseases which are less common but which lead to greater morbidity and mortality, such as eastern equine encephalomyelitis virus (EEE) and tetanus. This study consisted of two trials which investigated the antigenicity of commercially available vaccines licensed in the USA to protect against EIV, EHV-1 respiratory disease, EHV-1 abortion, EEE and tetanus in horses. Trial I was conducted to compare serological responses to vaccines produced by three manufacturers against EIV, EHV-1 (respiratory disease), EEE, and tetanus given as multivalent preparations or as multiple vaccine courses. Trial II compared vaccines from two manufacturers licensed to protect against EHV-1 abortion, and measured EHV-1-specific interferon-gamma (IFN-gamma) mRNA production in addition to serological evidence of antigenicity. In Trial I significant differences were found between the antigenicity of different commercial vaccines that should be considered in product selection. It was difficult to identify vaccines that generate significant immune responses to respiratory viruses. The most dramatic differences in vaccine performance occurred in the case of the tetanus antigen. In Trial II both vaccines generated significant antibody responses and showed evidence of EHV-1-specific IFN-gamma mRNA responses. Overall there were wide variations in vaccine response, and the vaccines with the best responses were not produced by a single manufacturer. Differences in vaccine performance may have resulted from differences in antigen load and adjuvant formulation. 相似文献
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Benbarek H Grülke S Deby-Dupont G Deby C Mathy-Hartert M Caudron I Dessy-Doize C Lamy M Serteyn D 《Equine veterinary journal》2000,32(4):327-333
We studied the interactions of isolated equine neutrophils with endothelial cells in culture, mimicking a situation of acute inflammation. Our main purpose was to demonstrate that the supernatant of activated neutrophils was sufficient to damage endothelial cells. Equine endothelial cells (from carotid arteries) were covered either with increased numbers of equine neutrophils stimulated by phorbol myristate acetate, or with the supernatant collected after an in vitro stimulation of the neutrophils. Cytotoxicity was estimated by the release of preincorporated 51Cr, and by light microscopy observations. To assert the specific role of reactive oxygen species, endothelial cells were treated by the hypoxanthine/xanthine oxidase (X/XOx) system (production of superoxide anion and hydrogen peroxide), and by hypochlorite (product of the activity of myeloperoxidase). A strong cytotoxicity was found with stimulated neutrophils; microscopic observations indicated a loss of 50% of the endothelial cells and morphological alterations in the remaining cells. The supernatant of stimulated neutrophils was cytotoxic, in correlation with the number of neutrophils used to obtain the supernatant, and with the supernatant concentration of myeloperoxidase. The cytotoxicity of the X/XOx system was weak, but was increased by myeloperoxidase. Hypochlorite was highly toxic. We concluded that the supernatant of stimulated neutrophils was sufficient to obtain cytotoxic effects on the endothelium, in the absence of a direct contact between endothelium and neutrophils, and that this cytotoxicity was mainly linked to the activity of myeloperoxidase. From these in vitro results, it can be extrapolated that in pathologies characterised by an important activation of neutrophils, damage can spread to cells and tissues away from the inflammation focus. 相似文献
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Biology of equine piroplasmosis 总被引：11，自引：0，他引：11
A A Holbrook 《Journal of the American Veterinary Medical Association》1969,155(2):453-454
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Pathology of equine phycomycosis 总被引：1，自引：0，他引：1
The pathological findings in three cases of equine phycomycosis in Costa Rica are described. Two cutaneous (Pythium sp) and one nasal lesion (Conidiobolus) were observed. 相似文献
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Transmission of equine sarcoid 总被引：1，自引：0，他引：1
J L Voss 《American journal of veterinary research》1969,30(2):183-191
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Methods of equine castration 总被引：1，自引：0，他引：1
C D Heinze 《Journal of the American Veterinary Medical Association》1966,148(4):428-432
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Rebecca Lyn Pond Michael J. Darre Peter M. Scheifele Dave G. Browning 《Journal of Veterinary Behavior: Clinical Applications and Research》2010,5(1):7-12
Bioacoustics is the study of sound in animals and includes, but is not limited to, animal communication with associated behavior, sound production anatomy and neurophysiology, auditory capacities and auditory mechanisms, and animal welfare. The present research investigates the vocalizations of horses during stressful situations. Stress can be positive or negative. Distress is anything that affects the animal in a negative way, such as in mare and foal separation. Eustress is anything that affects the animal in a positive way, such as morning feeding time in a horse barn. The purpose of the current research is to find spectral differences in the recorded vocalizations of stalled horses that indicate both distress and eustress using the Hidden Markov Model (HMM). Greenwood Function Cepstral Coefficient values suggest that there are spectral differences between vocalizations in a distress and eustress situation. These consistent results indicate that further research to obtain and evaluate vocalizations of horses may provide a productive tool in understanding equine welfare. 相似文献
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