共查询到19条相似文献，搜索用时 140 毫秒
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近年来,通过病毒分离鉴定,发现犬细小病毒性肠炎、猫泛白细胞减少症和水貂、貉出血性肠炎等细小病毒感染症,在我国的军犬、警犬、狮、虎、豹、猫以及水貂、貉等犬科、猫科、鼬科动物中广为存在,危害严重。为能迅速确定诊断,及时采取有效防疫 相似文献
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猫泛白细胞减少症又称为猫瘟热(以下简称猫瘟),是由猫泛白细胞减少症病毒(Feline panleukopenia virus,FPV)引起的猫及猫科动物的一种急性高度接触性传染病.临床表现为发热、呕吐、腹泻、脱水及白细胞严重减少和肠炎.FPV在自然条件下可以感染猫,也可以感染狐、狮、豹和浣熊等珍稀野生动物,给养猫爱好者和野生动物保护造成巨大威胁. 相似文献
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动物园猫瘟热净化的新手段—聚合酶链式反应 总被引：1，自引：0，他引：1
猫泛白细胞减少症病毒 (FPV)又名猫细小病毒、猫传染性肠炎病毒、猫瘟病毒。该病以高热、呕吐、白细胞严重减少和肠炎为特征。 1 957年 (Bilin)病毒首次被分离培养成功。通过对多种动物类似疾病的病原学研究 ,证明 FPV在自然条件下感染猫科和鼬科多种动物 ,如虎、豹、狮子和浣熊 ,但以体型较小的猫科动物 ,包括水貂最为易感。FPV是目前本属病毒中感染范围最宽、致病性最强的一种。由FPV引起的猫泛白细胞减少症 ,可能存在于世界各地。在欧洲和北美已被认为是猫的重要传染病之一。我国张振兴和李刚等人 (1 982— 1 984)报道 ,我国许多省… 相似文献
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猫泛白细胞减少症(又称猫传染性肠炎，俗称猫瘟)是一种高度接触性、急性、致死性、病毒性传染病，其主要特征为高热、呕吐、腹泻，白细胞减少。病原为猫泛白细胞减少症病毒。本病主要感染猫，一岁内幼猫易感，冬春两季多发。除猫外，猫科其他成员：如野猫、虎、豹、猞猁、山猫、及浣熊等均可感染，可经消化道直接或间接接触感染。猫和康复猫是主要传染源。在发病的急性期，跳蚤和吸血昆虫可作为传染媒介。笔者在2003年某动物门诊收治一例，具体诊治情况如下，供同行参考。 相似文献
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从沈阳市动物园猫科动物猞猁等疑似细小病毒性肠炎流行期,采集了三份病料,利用猫肾传代细胞(FK)采田同步接毒法分离到2株细小病毒,经透射电镜、免疫电镜和血清学HA、HI试验证实与猫泛白细胞减少症病毒(FPY)的特性基本相符,将第6代分离物(FKV_6)回归本属动物,人工发病获得成功,从而首次确认本病在我省的存在。 猫科动物病毒性肠炎,又名猫瘟热症,病原为猫泛白细胞减少症病毒(FPV),属细小DNA病毒,本病是对猫科动物危害极大的一种病毒性传染病,除猫以外,FPV可感染虎、狮、豹、猞猁等多种猫科动物,该病原广泛存在自然界中,幼令猫科动物对本病的感染率为70%,死亡率为50—60%甚至高达90%。对沈阳市动物园猫科动物1985年流行本病后进行统计,发病率为59%,致死率为53.8%。我们从发病死亡的三只猞猁肠内容物和脏器中分离到的二株病毒,经免疫电镜,HA、HI试验及生物学试验等方法进行鉴定,证明所分离到的二株病毒均为FPV,现报告如下。 相似文献
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猫泛白细胞减少症病毒(Feline pauleukopinia virus,FPV)同义名为猫细小病毒,猫传染性肠炎病毒,猫瘟病毒[1].以下简称"猫瘟病毒".该病是猫的一种急性/致死性白细胞减少.我国许多省市区均有发生本病流行的的报道.1985年南京农业大学兽医系从病体内分离病毒成功[3].在自然条件下,猫瘟病毒除感染猫外,还侵害虎、豹、狮、熊猫、鼬等动物,发病和死亡率较高[1、6].在疫苗接种方面,对本病能产生一定效果,但由于诸多原因,该病发生和流行仍呈上升势头,迄今国内外尚未见到较理想的有效治疗药物.有鉴于此,我们因地制宜,选用陕西关中富有的特产大型毛驴作为免疫动物[5],研制出用于预防和治疗猫瘟病毒性肠炎的有效药物-抗猫瘟免疫球蛋白(IgG)生物制剂,经过五年反复试验,终于获得成功. 相似文献
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林逢生 《中国预防兽医学报》1988,(6)
引起猫、水貂及犬肠炎的病毒分别为猫泛白细胞减少症病毒、水貂病毒性肠炎病毒及犬细小病毒。这三种病毒均属于细小病毒科、细小病毒属。近年来,在安徽、河南、山东等地连续爆发家猫、云豹等猫科动物由猫泛白细胞减少症病毒引起的猫泛白细胞减少症。在黑龙江、辽宁等省的许多水貂场不断发生由水貂病毒性肠炎病毒引起的水貂病毒性肠炎。由犬细小病毒引起的犬、貂和狐狸的犬细小病毒肠炎,在全国许多地方亦有 相似文献
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桂林老虎猫瘟热病毒的分离鉴定 总被引：5，自引：0，他引：5
我们在作猫细小病毒分子流行病学调查过程中,从桂林送棼的考虑粪便中分离出１株虎细小病毒,并对其进行了系统鉴定,经形态学理化学血清学交叉中和试验、动物感染试验与分子生物学,证明为一株猫瘟热病毒（猫泛白细胞减少症病毒）的强毒。 相似文献
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用PCR法检测东北虎感染猫细小病毒的研究 总被引：3，自引：0，他引：3
根据GenBank中已发表的猫细小病毒基因组中的保守序列 ,利用Goldkey软件设计了一对能扩增 750bp片段大小的引物 ,对某动物园 1只病死东北虎的脾脏样品进行了PCR检测。结果得到了与设计大小完全相符且与标准猫细小病毒扩增产物大小一致的特异核酸带 ,同时对犬瘟热、犬腺病毒、轮状病毒的核酸扩增结果均为阴性。敏感性试验表明 ,此法可检出血凝价为 1 2 8×脾脏匀浆液上清 1 0 - 5倍稀释的模板 ,远高于血凝试验的敏感性 ,为虎、狮、熊猫等野生动物细小病毒病的快速诊断提供了一个有效的方法 相似文献
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虎源猫瘟热病毒VP2蛋白特异性单克隆抗体的制备 总被引：1，自引：1，他引：0
以原核表达的虎源猫瘟热病毒(FPV)VP2蛋白作为免疫抗原,免疫6周龄BALB/c小鼠,用FPV全病毒作为筛选抗原,采用淋巴细胞杂交瘤技术,经过有限稀释法获得了1株可稳定分泌抗虎源FPVVP2蛋白的杂交瘤细胞株3C4。间接ELISA方法测定3C4株单抗腹水效价为1∶12800,亚类鉴定为IgG2a类型,间接免疫荧光试验显示该株单抗能与虎源FPV发生反应,而免疫印迹试验该株单抗未见特异性反应带。 相似文献
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Sassa Y Yamamoto H Mochizuki M Umemura T Horiuchi M Ishiguro N Miyazawa T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):491-494
Feline parvoviruses were isolated from frozen samples of intestines taken from a snow leopard (Uncia uncia) and a serval (Leptailurus serval) that died successively at Sapporo Maruyama Zoo in Hokkaido, Japan. Isolates possessed an antigenic epitope for both the feline panleukopenia virus (FPLV) and mink enteritis virus, identified with a hemagglutination inhibition test. Sequencing analyses of the VP2 region of the isolates revealed that the two isolates were identical and of the FPLV-type. These results suggested that FPLV was introduced from a feral cat which entered the zoo and transmitted the virus inside the zoo. 相似文献
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M Mochizuki H Kawabata W J Sheng 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(2):99-105
Fourteen feline parvovirus (FPV) strains isolated from cats, mink and dogs were comparatively examined on their antigenic and genetic diversities by using monoclonal antibodies against feline panleukopenia virus (FPLV) and restriction enzyme analysis of viral DNA. Mink enteritis virus (MEV) strains recently isolated in the northeastern area of the People's Republic of China were found to possess more similar antigenic and genetic properties to the antigenic variant virus of canine parvovirus (CPV) ("new" antigenic type CPV), than to FPLV strains and MEV Abashiri strain of Japan. A feline isolate detected in normal cat feces was considered to be rather CPV because of its antigenic and genetic characteristics. An early isolate of "new" antigenic type CPV strains showed a similar cleavage pattern to those of "old" antigenic type CPV strains when digested with HinfI. The results including some features above-mentioned suggest the presence of antigenic heterogeneities and genomic polymorphisms among FPV subspecies viruses. 相似文献
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从临床表现有体温升高、呕吐、血样腹泻、脱水等症状的疑似猫泛白细胞减少症感染的病例采取粪样28份。从粪便样品中成功分离获得了7株猫泛白细胞减少症病毒（FPV）：JX-1、JX-2、JX-3、JX-4、JX-5、JX-6和JX-7;应用F81细胞增毒,盲传至3代时在F81细胞上产生细胞病变(脱落、变形、游离等);核酸型鉴定证明,FPV毒株的代谢可被5-IUDR所抑制,其核酸属于DNA型;所分离的病毒培养物能凝集猪的红细胞（凝集效价达26～28）,并能被标准FPV阳性血清所抑制;电镜观察病毒粒子外观呈圆形或六边形,直径20～30 nm;该病毒耐酸、耐热、耐乙醚;动物致病性试验,经口感染分离细胞培养毒1 ml,试验组幼猫第7 d发病,采集病猫粪样做HA试验为阳性反应,做HI试验,其凝集猪红细胞的能力被抑制。 相似文献
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Response of mink, skunk, red fox and raccoon to inoculation with mink virus enteritis, feline panleukopenia and canine parvovirus and prevalence of antibody to parvovirus in wild carnivores in Ontario. 
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下载免费PDF全文 Mink virus enteritis, feline panleukopenia and canine parvovirus-2 were inoculated separately into groups of raccoon, mink, red fox and striped skunk. Raccoons were highly susceptible to mink virus enteritis and feline panleukopenia, with animals developing clinical illness, and several dying within six to ten days of inoculation with lesions typical of parvovirus infection. Both viruses were shed in high titre in the feces of infected raccoons, and high antibody titres were stimulated. Raccoons inoculated with canine parvovirus-2 showed no signs; shedding of virus was sporadic though moderate titres of antibody developed. Mink inoculated with mink virus enteritis and feline panleukopenia developed signs and lesions of early parvovirus infection. No signs or significant lesions followed canine parvovirus-2 inoculation. Shedding of virus was heavy (mink virus enteritis) or sporadic (feline panleukopenia and canine parvovirus-2), though good serological responses were elicited to all three viruses. Red fox showed no signs of infection, shed all three viruses only sporadically, and the serological response was strong only to feline panleukopenia. Skunks developed low antibody titres, but no signs, and did not shed virus. Antibody to parvovirus was found in 79.2% of 144 wild red foxes; 22.3% of 112 wild raccoons; 1.3% of 157 wild skunks and 6/7 coyotes in southern Ontario. The likely significance of these viruses to wild and captive individuals and populations of these carnivores is discussed. 相似文献
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Battilani M Balboni A Ustulin M Giunti M Scagliarini A Prosperi S 《Veterinary research》2011,42(1):43
ABSTRACT: Parvoviruses of carnivores include three closely related autonomous parvoviruses: canine parvovirus (CPV), feline panleukopenia virus (FPV) and mink enteritis virus (MEV). These viruses cause a variety of serious diseases, especially in young patients, since they have a remarkable predilection for replication in rapidly dividing cells. FPV is not the only parvovirus species which infects cats; in addition to MEV, the new variants of canine parvovirus, CPV-2a, 2b and 2c have also penetrated the feline host-range, and they are able to infect and replicate in cats, causing diseases indistinguishable from feline panleukopenia. Furthermore, as cats are susceptible to both CPV-2 and FPV viruses, superinfection and co-infection with multiple parvovirus strains may occur, potentially facilitating recombination and high genetic heterogeneity. In the light of the importance of cats as a potential source of genetic diversity for parvoviruses and, since feline panleukopenia virus has re-emerged as a major cause of mortality in felines, the present study has explored the molecular characteristics of parvovirus strains circulating in cat populations. The most significant findings reported in this study were (a) the detection of mixed infection FPV/CPV with the presence of one parvovirus variant which is a true intermediate between FPV/CPV and (b) the quasispecies cloud size of one CPV sample variant 2c. In conclusion, this study provides new important results about the evolutionary dynamics of CPV infections in cats, showing that CPV has presumably started a new process of readaptation in feline hosts. 相似文献